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Investigation of Immunoregulation in Reducing Foodborne Pathogen Colonization in Poultry

Investigators
Swaggerty, Christina; Kogut, Michael; He, Haiqi; Genovese, Kenneth
Institutions
USDA - Agricultural Research Service
Start date
2016
End date
2021
Objective
Objective 1: Define the differential host-pathogen interactions between Salmonella and chicken and poultry mucosal immune systems using genomic technologies. Sub-objective 1.A. Screen two lines of chickens and turkeys to identify individual sires and dams that have inherently higher and lower levels of key pro-inflammatory cytokines/chemokines (IL-6, CXCLi2, and CCLi1) and perform specific matings to produce a high and low line of chickens/turkeys and evaluate this novel selection method for increased resistance against Salmonella enterica serovar Enteritidis. Sub-objective 1.B. Evaluate the mucosal immune response and gut microbiome in differentially selected immune lines of chickens and turkeys. Objective 2: Determine the relationship between foodborne pathogens and the mucosal innate immune response focusing on epigenetic reprogramming of host immune genes in persistent infections. Objective 3: Develop new vaccination strategies based on innate immunity to reduce Salmonella contamination in broiler chickens and turkeys. Objective 4: Develop strategies to reduce foodborne pathogens by targeting host immune-metabolic signaling pathways affected by Salmonella and Campylobacter virulence factors. Sub-objective 4.A. Characterize the immune-metabolic pathways through which Salmonella and Campylobacter infection induce a local "tolerogenic" environment in the intestine that controls T regulatory cell development and mediates long-term persistent infection. Sub-objective 4.B. Characterize the immune-metabolic signaling pathways in the ceca of chickens and turkeys treated with various immune modulators that protect birds against Salmonella and Campylobacter infections. Objective 5: Analyze and characterize both host and Salmonella proteins that are modulated in expression during infection using quantitative proteomics. Sub-objective 5.A. Construct a Salmonella proteomic array to identify common Salmonella-specific antigen targets using immune sera from chickens and turkeys infected with different serovars of Salmonella. Sub-objective 5.B. Develop a high-throughput assay to screen small molecules for their ability to inhibit virulence factors produced by various serovars of Salmonella enterica.
Funding Source
Agricultural Research Service
Project source
View this project
Project number
3091-32000-034-00D
Accession number
430194
Categories
Campylobacter
Bacterial Pathogens
Commodities
Meat, Poultry, Game