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Laboratory Detection of Toxoplasma in the Environment

Investigators
Dubey, Jitender
Institutions
USDA - Agricultural Research Service
Start date
2002
End date
2005
Objective
To develop test for the detection of Toxplasma gondii in environmental water supplies.

Toxoplasma gondii oocysts will be obtained from feces of experimentally infected cats, purified by sucrose-gradients. Oocysts will be sporulated in 2% sulfuric acid as preservative and then stored at 4 C until used. Known numbers of oocysts will be mixed with water samples and then sensitivity of various methods (bioassay, PCR, monoclonal antibodies) will be tested.

Funding Source
Agricultural Research Service
Project number
1265-32000-066-13R
Accession number
405983
Categories
Toxoplasma