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Mechanistic Evaluation of the Induction of Lymphoproliferation and Apoptosis Inhibition by Probiotic Bacteria in Mice Infected with Salmonella Enterica

Investigators
Wagner, Robert
Institutions
DHHS/FDA - National Center for Toxicological Research
Start date
2011
End date
2012
Objective
1) Orally challenge defined human microbiota-associated (HMA) BALB/c mice and probiotic-bacteria-treated HMA BALB/c mice with Salmonella enterica and isolate intestinal mucosal-associated lymphoid tissues (MALT) , including Peyer's patches, lamina propria, and mesenteric lymph nodes.

2) Use pathway-focused gene-expression profiles generated from real-time RT-PCR expression arrays to compare signal transduction in MALT from HMA mice treated with or without probiotic bacteria and orally challenged with S. enterica.

3) Develop immunohistochemical (IHC) and in situ hybridization (ISH) conditions to detect the expression of the signal pathway molecules implicated in activation and apoptosis inhibition in mucosal T cells and accessory cells in tissue sections of Peyer's patches, lamina propria, and mesenteric-lymph nodes.

4) Conduct IHC and ISH studies on tissue sections for detection of molecules involved in the regulation of lymphocyte activation and programmed cell-death pathways induced by bacterial-surface antigens.

5) Compare the probiotic-treated and untreated mice for expression of dendritic cell, macrophage, and IEC-derived cytokines.

More information
Responsible Division: Microbiology
Funding Source
Food and Drug Administration
Project source
View this project
Project number
E0727601
Categories
Chemical Contaminants
Parasites
Natural Toxins
Viruses and Prions
Bacterial Pathogens
Salmonella