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Methods for the Detection of Enteroaggregative Escherichia coli

Institutions
Campden BRI
Health Protection Agency
HPA Colindale
Start date
2002
End date
2004
Objective
This research project aims to develop a detection method for Enteroaggregative E. coli that can be used to screen clinical, food and environmental samples without the need to refer them to a specialist laboratory.

The project aims to develop a multiplex PCR method able to detect EAEC strains from patient faecal material and food samples.

More information
The Enteroaggregative E. coli (EAEC) are a diverse group of the enterovirulent E. coli and cause Infectious Intestinal Disease (IID) in humans.

A Department of Health funded study of IID in England (the IID Study) demonstrated that EAggEC are a significant cause of IID in humans. EAEC were isolated from 5.1% of cases presenting to GPs and 2.9% of cases in the community.

Our knowledge of the pathways by which EAEC are transmitted to humans is limited by the lack of widely available methods for detecting these bacteria.

Detection of EAEC in clinical or food samples is currently limited to specialist laboratories that conduct cell adhesion assays or use gene probes to detect the aggregative adhesion phenotype.

Work was needed to develop a detection method for EAEC that can be used to screen clinical, food and environmental samples without the need to refer them to a specialist laboratory for testing.

Find more about this project and other FSA food safety-related projects at the Food Standards Agency Research webpage.

Funding Source
Food Standards Agency
Project number
B14002
Categories
Escherichia coli