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Microbial Diversity Analysis

Investigators
Rooney, Alejandro (Alex)
Institutions
USDA - Agricultural Research Service
Start date
2006
End date
2007
Objective
The overall objective for this project is to develop new methods for the analysis of genetic diversity between closely related species of bacteria and between distinct strains within those species in order to improve methods of detection and surveillance.

These developments will enhance existing technologies designed to rapidly detect and identify microorganisms of importance to public health and safety. The results from this project will be generally applicable to a wide range of microorganisms of importance in agriculture.

More information
Approach: Multilocus DNA sequence databases will be developed for select bacterial species. These data will be combined with data from complete genome sequences and analyzed in order to provide an evolutionary framework for the rapid detection, identification and surveillance of these species. This evolutionary framework, along with information concerning the geographic distribution of strains and the substrates from which they were isolated, will be used to develop DNA sequence-based subtyping technologies that will enhance the detection, surveillance and source-tracking efforts applied to these microorganisms.
Funding Source
Agricultural Research Service
Project number
3620-42000-037-02R
Accession number
411171
Categories
Risk Assessment, Management, and Communication
Chemical Contaminants
Microbiological Standards and Guidelines