An official website of the United States government.

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Molecular Basis of Salmonella Virulence

Institutions
Institute for Animal Health
Start date
2001
End date
2004
Objective
The Group has recently identified and characterised and currently is working on TTSS-1 secreted effector proteins (termed Sops for Salmonella outer proteins or Pips for Pathogenicity-island-encoded proteins) which are key virulence factors required for Salmonella invasion, induction of fluid secretion and inflammatory responses.

Our objective is to understand how different Salmonella Sop and Pip effector proteins function inside host cells to elicit proinflammatory responses that lead to intestinal inflammation. We speculate that some Sops and Pips may be needed for the induction of signals leading to the attraction of PMN from the peripheral blood into the lamina propria, whereas, others may be involved in orchestrating the migration of PMNs across the epithelial monolayers.

We will construct and use genetically defined Salmonella mutants in association with our biologically relevant model systems to examine the importance of effector proteins in enteropathogenicity. To enable further functional analyses, we will assemble eukaryotic expression plasmids encoding Sop and Pip effector proteins and assess effects mediated by their expression in cultured cells. We will localize effector proteins to specific cellular organelles and analyze the targeting signals and subsequent cellular responses. The rationale for this is based on the knowledge that different effector proteins (Yops) of Yersinia are targeted to different cellular compartments.

Finally, we will assess the relevance of the data obtained in vitro by performing experimental Salmonella infections using the relevant animal model system and monitoring the responses elicited by such infections.

Funding Source
Biotechnology and Biological Sciences Research Council
Project number
BBSEI00000858
Categories
Salmonella
Viruses and Prions
Sanitation and Quality Standards