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Nanoparticle-based Fluorescent Ibosensor for Rapid Detection of Listeria Monocytogenes in Foods

Investigators
Paoli, George
Institutions
University of Arkansas
USDA - Agricultural Research Service
Start date
2006
End date
2007
Objective
The goal of the proposed project is to develop an optical biosensor, based on innovative nanoparticle bioseparator and quantum dots biolabels, for detection of L. monocytogenes in food products including poultry, meat and vegetables. It is expected that the proposed biosensor will be able to detect one cell of viable L. monocytogenes in food samples in less than two hours.
More information
APPROACH:
1. Develop a novel nanoparticle bioseparator by coupling magnetic nanoparticles with anti-Listeria antibodies for high-efficiency separation of target pathogens from food matrix.
2. Develop novel nanoparticle biolabels by coupling semiconductor quantum dots (QDs) with anti-L. monocytogenes antibodies for very sensitive labeling of target pathogens with fluorescence.
3. Integrating the nanoparticle bioseparator, QDs biolabels, and fluorescent detector into a biosensing system for rapid screening of L. monocytogenes in foods.
Funding Source
Agricultural Research Service
Project number
1935-42000-058-03S
Accession number
411073
Categories
Listeria
Commodities
Meat, Poultry, Game