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A Novel Genome-Wide Evaluation of Mutants in Salmonella Enterica Serovar Typhimurium: Application to Drug and Vaccine Development

Institutions
University of Oxford
University of Newcastle upon Tyne
University of Cambridge
Start date
2003
End date
2006
Objective
  1. To generate mutant libraries using two novel genome- wide technologies (TMDH and ODM) developed by Arrow Therapeutics.
  2. To identify genes important for bacterial survival, and thereby possible candidates for anti-microbial drug discovery. Attenuated mutants will be evaluated as candidate lives vaccines. The use of S. typhimurium allows the evaluation of survival of mutants in a real infection in an animal host, and thus defines unequivocally the minimum gene set required for virulence and persistence during infection.
  3. To select genes identified by TMDH and ODM for recombinant expression and characterisation. This approach utilises a set of proprietary bioinformatics tools along with protein purification, crystallisation methodologies, and structural determination to evaluate rapidly proteins for the drug discovery process.
Funding Source
Biotechnology and Biological Sciences Research Council
Project number
APG19114/APG19115/APG19116
Categories
Bacterial Pathogens