An official website of the United States government.

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Optical Biosensor Detection of Food Pathogens Based on Direct Measurement of Antibody/Antigen Binding (1999-02902)

Investigators
Brown, Christopher; Goldberg, Bennett; Pivarnik, Lori; Letcher, Steven; Rand, Arthur
Institutions
University of Rhode Island
Start date
1999
End date
2001
Objective
The demands from consumers for fresh, less processed food has increased the need to ensure microbial safety of these products. This project provides the opportunity for a multi-disciplinary effort to create specific biosensors for rapid andearly detection of pathogen contamination.
More information
These devices have the potential for specifically selecting food pathogens fromamong the total microbial load within minutes and measuring the concentration as real-time analysis on site. The goal ofthis project is to develop new prototype biosensor devices that will consist of either a fiber optic fluorometer and magneticfocusing system or a new evanescent thin-film wave guide for the transducers. One advance will be the creation of an immunosensing element which consists of unique new complexes of antibodies attached to solvatochromic fluorescent dyes. This new dye/antibody complex will be designed to detect cell attachment directly when immobilized on magnetic microspheres or thin-film wave guides. The coupling of direct measurement antibodies with laser excitation will provide the basis for rapid one-step, sensitive, and easy-to-use biosensors for detection of food pathogens. The ability for rapid early detection of Salmonella will enhance the safety and quality of U.S. food products.
Project number
99-35201-8435
Categories
Prevention and Control
Bacterial Pathogens
Salmonella
Predictive Microbiology