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Pragmatic Separation of E. Coli from Agricultural Water for Rapid Detection

Investigators
Nugen, Sam
Institutions
Cornell University
Start date
2018
End date
2021
Objective
The overall objective of this research proposal is to enable low-cost phage-based magnetic separation of bacteria from liquid samples by developing a pragmatic means to conjugate phage capsids to magnetic particles. Therefore, the aims of this project will include 1) engineering alkyne-functionalized T7 for conjugation onto azide-functionalized nanoparticles, allowing the magnetized phages to bind and separate E. coli from liquid samples, and 2) a validation study to determine the efficiency of E. coli capture from drinking and irrigation water. The inclusion of the luciferase gene will also allow rapid detection of the separatedE. coli using either dipsticks and a custom reader, or a plate reader available in the Nugen lab.[34-36]
Funding Source
Nat'l. Inst. of Food and Agriculture
Project source
View this project
Project number
NYC-143416
Accession number
1017700
Categories
Escherichia coli
Bacterial Pathogens
Natural Toxins