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Prevalence, Persistence and Control of Non-O157 Shiga Toxin Producing Escherichia coli

Investigators
Warriner, Keith
Institutions
University of Guelph
Start date
2011
End date
2012
Objective
1. To perform a comparative study on detection techniques for the Top Six STEC serotypes.

2. Determine the prevalence of the Top Six serotypes and stx2 carrying E. coli in representative beef processing facilities within Ontario.

3. Determine the carriage of the Top Six and stx2 carrying E. coli in retail ground beef sold within Ontario.

4. Determine the inactivation kinetics of the Top Six serotypes when exposed to acid, thermal and high hydrostatic pressure.

More information
Expected Benefits:
Non-O157 STEC encompasses over 200 serotypes with six being implicated in fatal cases of HUS. This has led to FSIS to class the Top Six serotypes as adulterants. As a consequence, there is a need to determine the prevalence of the Top Six in Ontario beef processing and carriage in ground beef at retail. In addition, there is a need to advise industry on the relative performance of diagnostic tests to screen for the Top Six non-O157 E. coli STEC. In addition, it is important to verify that interventions for E. coli O157 are capable of controlling the pathogens.
Funding Source
Ontario Min. of Agriculture Food and Rural Affairs
Project number
SF6096
Categories
Escherichia coli
Bacterial Pathogens