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Studies on Mechanism of Fluoroquinolones Resistant Salmonella spp. Isolated from Animal Feeds (Poultry), Animal Production Environment and the Development of Molecular Methods for Screening the Drug Resistance Genes

Investigators
Khan, Ashraf
Institutions
DHHS/FDA - National Center for Toxicological Research
Start date
2000
End date
2001
Objective

1) To isolate, identify and characterize nalidixic acid and fluoroquinolone-resistant Salmonella spp. from chicken farms (animal feed, feces, manure,litters, and animals) by biochemical and polymerase chain reaction. 2) To determine minimum inhibitory concentration for environmental isolates, development of molecular techniques and its comparison with clinical strains. 3) To determine drug-resistance mechanisms in the environmental isolates and their characterization by molecular techniques. 4) To determine influence of seasons and the frequency of isolation of fluoroquinolone-resistant Salmonella spp.

More information
FY 2000 Accomplishments: 1) Developed multiplex PCR method for the rapid detection of multidrug-resistant Salmonella typhimurium. 2) Developed mismatch amplification mutation assy for the detection of ciprofloxacin-resistant Salmonella spp. 3) Isolated several nalidixic acid (antibiotic)-resistant E. coli from chicken and turkey farms. 4) Characterized fluoroquinolones-resistant Campylobacter spp. From chicken and turkey farms by pulse-field gel electrophoresis. FY2001 Plans: 1) Isolation and molecular characterization of fluoroquinolone-resistant Salmonella spp. And E. coli from chicken and turkey litters. 2) Determine the influence of climatic conditions on the occurrence of quinolone-resistant Salmonella spp. And E. coli.
Funding Source
Nat'l. Center For Toxicological Research
Project number
E0704801
Categories
Salmonella
Antimicrobial Resistance
Commodities
Meat, Poultry, Game