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Understanding Pycnidiospore Development, Mycotoxin Production, and The Primary Inoculum of Stenocarpella Maydis

Investigators
Woloshuk, Charles
Institutions
Purdue University
Start date
2016
End date
2021
Objective
The goal of the proposed research is to increase the knowledge base on the under-researched pathogen S. maydis. The host-pathogen interaction appears to be distinctive, notably the vital role of pycnidia in the disease cycle and the infection process of a seed pathogen. Development of a molecular system to study S. maydis will provide novel information that will contribute towards our understanding of pathogenesis, mycotoxin production, host resistance mechanisms, and fungicide efficacy.Objective 1. Generate and characterize mutants defective in diplodiatoxin biosynthesis and pycnidial development. We will use REMI (Restriction Enzyme Mediated Integration) as well as specific gene disruptions to generate mutants of S. maydis that can be used to better understand toxin production and reproductive aspects of this fungus.Objective 2. Profile gene expression in distinct stages of pycnidial development. We will use next generation sequencing technology to generate data that can be used for gene discovery.
Funding Source
Nat'l. Inst. of Food and Agriculture
Project source
View this project
Project number
IND011264
Accession number
1010718
Categories
Chemical Contaminants
Mycotoxins
Pesticide Residues