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Validation of a highly discriminatory molecular subtyping tool for Salmonella Enteritidis based on a single nucleotide polymorphism-polymerase chain reaction (SNP-PCR)

Investigators
Ogunremi, Dele
Institutions
Canadian Food Inspection Agency
Start date
2014
End date
2016
Objective

The goals of the proposals are:

  1. To demonstrate use of the newly developed molecular subtyping test to show epidemiological relationship among isolates of SE.
  2. To carry out a validation of the test procedure by applying it to a large number of SE isolates (n = 1,000) and develop quantitative parameters of test performance.
  3. To carry out inter-laboratory test validation
  4. To construct a "Canadian map of SE" using molecular subtyping tool to trace, establish relatedness and identify transmission pathways of isolates found in various sources such as retail poultry products, live poultry, poultry production environment, hatchery, animal feed, wildlife, and humans).

In summary, the successful completion of the proposal should provide a validated tool that can be used to accurately and reliably monitor, trace and establish relationships or a lack thereof, among isolates of Salmonella Enteritidis. This tool will improve the control of SE.

More information

Current mitigation strategies to deal with sources of foodborne Salmonella Enteritidis (SE) outbreaks, e.g., food recall, rely on molecular subtyping methods such as pulsed-field gel electrophoresis and phage typing. Both tests show low discriminatory power and sometimes poor reproducibility mainly because of the unprecedented, high genetic relatedness among SE isolates. A previous OMAFRA-funded project (FS090507/FS6080) on whole genome analysis led to the identification of single nucleotide polymorphism among SE isolates, and has resulted in the development of a tool previously unavailable: an inexpensive, rapid, robust and highly discriminatory test for SE, designated as SE-SNP-PCR. The new objectives are (a) to use the SE-SNP-PCR to provide laboratory evidence of relatedness among SE isolates deemed to have epidemiological relationships (b) validate the test, and (c) carry out interlaboratory testing to verify test reproducibility. A validated SE-SNP-PCR will be available to laboratories and food safety managers tasked with SE control.

Funding Source
Ontario Min. of Agriculture Food and Rural Affairs
Project source
View this project
Project number
FS2013-1857
Categories
Salmonella
Parasites
Natural Toxins
Viruses and Prions
Bacterial Pathogens
Chemical Contaminants