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Validation of Testing Methods for the Detection and Quantification of Escherichia Coli 0157:H7, Salmonella SPP

Institutions
USDA - Agricultural Research Service
Start date
2012
End date
2013
Objective
The objective of this study is to evaluate TMECC and EPA pathogen detection methods in order to accurately determine the presence of populations of E. coli O157:H7, Salmonella, non-pathogenic E. coli, and fecal coliforms in ‘point of sale’ composts collected from commercial operations across the United States. Correlations between the presence of pathogens and several physico-chemical parameters (moisture, water activity, electrical conductivity, soluble carbon, pH, and carbon:nitrogen ratios) will be investigated.
More information
Approach: ARS will acquire the basic knowledge about the stresses that pathogenic E. coli or Salmonella strains undergo in compost and their prevalence in various types of compost. The carbon:nitrogen ratio will evaluated in commercial formulations. Based on these findings, compost formulations using specific C:N ratios will then be formulated to determine survival and inhibition of E. coli and Salmonella.
Funding Source
Center for Produce Safety
Project source
View this project
Project number
2012-224
Categories
Escherichia coli
Predictive Microbiology
Salmonella