Enzyme and Microbial Technology

The Research Publications track research that is published across national and international peer-reviewed journals. The most recent articles are available ahead of print and searchable by Journal Title, Subject, USDA Grant Funding Agencies or FDA Grant Funding Agencies. The research publications are tracked across five subject areas: Bacterial Pathogens, Chemical Contaminants, Natural Toxins, Parasites, and Viruses.

Sun, 06/28/2020 - 06:06
Author(s): Heelak Choi, Sung-Jin Han, Jong-In Won Elastin-like polypeptides (ELPs) are stimulus–responsive protein-based biopolymers that exhibit phase transition behavior. By joining them to synthetic phytochelatin (EC), EC–ELP fusion proteins with temperature sensitivity and metal-binding functionality were generated to remove heavy metal ions biologically. Three different EC domains (EC10,...
Sun, 06/07/2020 - 06:05
Author(s): Yaping Gao, Yun He, Igor Ivanov, Xuerui Yang, Hui Tian, Xing Liu Halophilic organisms are found widely in environments where the salt concentration is higher than 0.2 M. Halophilic proteins isolated from these organisms maintain structural integrity and function under high salt stress, whereas their non-halophilic homologs tend to aggregate and collapse. Here we report for the first...
Mon, 02/10/2020 - 03:35
Author(s): Jing Yang, Dexin Xu, Wenchen Yu, Ruobing Hao, Jie WeiAbstractaurJ3M(GenBank:EU697915.1), a 579 bp gene, whose deduced product (192 amino acid) was found to have amino acid sequence homology with some bacterial regulatory proteins. Computer-assisted analysis showed that AurJ3M is PAS-LuxR family regulatory protein, it combines a PAS domain with a helix–turn–helix (HTH) motif of the LuxR...
Mon, 02/10/2020 - 03:35
Author(s): Mei Cheng, Yingying Zhu, Ziwei Chen, Cuie Guang, Wenli Zhang, Wanmeng MuABSTRACTInulin is a widespread polysaccharide, and it can be bio-transferred to α-D-fructofuranose-β-D-fructofuranose 1,2′:2,1′-dianhydride (DFA I), a potential low-calorie sweetener, via inulin fructotransferase (IFTase, EC 4.2.2.17). In this study, a novel DFA I-forming IFTase was identified and characterized...
Wed, 01/22/2020 - 03:20
Author(s): Haikun Zhang, Jingyi Xie, Yanyu Sun, Ailing Zheng, Xiaoke HuAbstractAntimony trisulfide (Sb2S3) is industrially important for processes ranging from a semiconductor dopant through batteries to a flame retardant. Approaches for fabricating Sb2S3 nanostructures or thin films are by chemical or physicochemical methods, while there have been no report focused on the biological synthesis of...
Thu, 01/16/2020 - 03:19
Author(s): Karla Ilić Đurđić, Raluca Ostafe, Aleksandra Đurđević Đelmaš, Nikolina Popović, Stefan Schillberg, Rainer Fischer, Radivoje ProdanovićAbstractAzo dyes are toxic and carcinogenic synthetic pigments that accumulate as pollutants in aquatic bodies near textile factories. The pigments are structurally diverse, and bioremediation is mostly limited to single dye compounds or related groups....
Sat, 12/14/2019 - 03:21
Author(s): Jong Min Yang, Gi Eob Kim, Kyeong Rok Kim, Chang Sup KimAbstractThe successful enzymatic synthesis of various ganglioside-related oligosaccharides requires many available glycan-processing enzymes. However, the number of available glycan-processing enzymes remains limited. In this study, the full-length CgtA43456 (β-(1→4)-N-acetylgalactosaminyltransferase) and CgtB11168 (β-(1→3)-...
Fri, 11/01/2019 - 03:21
Author(s): Hong-Rae Kim, Byoung-Gi An, Young Wook Chang, Min-Jung Kang, Jae-Gwan Park, Jae-Chul PyunAbstractHighly sensitive in situ-synthesized cadmium sulfide (CdS) nanowires (NWs) for the detection of chemiluminescence in immunoassays with a photoresist (PR) layer to stabilize the CdS NWs before and after coating with a parylene film were developed. The thickness of the PR layer was controlled...
Mon, 10/21/2019 - 03:25
Author(s): Lorena Cardoso Cintra, Isabelle Cristine da Costa, Izadora Cristina Moreira de Oliveira, Amanda Gregorim Fernantes, Syd Pereira Faria, Rosália Santos Amorin Jesuíno, Maria Cristina Ravanal, Jaime Eyzaguirre, Luiz Pereira Ramos, Fabrícia Paula de Faria, Cirano José UlhoaAbstractTo increase the efficiency of enzyme cocktails in deconstructing cellulose and hemicelluloses present in the...
Thu, 10/17/2019 - 03:25
Author(s): Sunirmal Sheet, Yesupatham Sathishkumar, Kuntal Ghosh, Mi-Sook Choi, Kwan Seob Shim, Yang Soo LeeAbstractThe present study investigated the influence of low-shear modeled microgravity (LSMMG) conditions on Listeria monocytogenes stress response (heat, cold, and acid), membrane fatty acid composition, and virulence potential as well as stress-/virulence-associated gene expression. The...
Tue, 09/24/2019 - 03:23
Author(s): A.S. Kharkova, V.A. Arlyapov, A.D. Turovskaya, V.I. Shvets, A.N. ReshetilovAbstractA mediator biosensor based on Paracoccus yeei bacteria for assaying the toxicity of perfumery and cosmetics samples was developed. An approach to selecting an electron-transport mediator based on the heterogeneous electron transfer constants for investigated mediators (ks) and the mediator–biomaterial...
Tue, 09/24/2019 - 03:23
Author(s): Sha Xu, Yu Zhang, Youran Li, Xiaole Xia, Jingwen Zhou, Guiyang ShiAbstractL-tyrosine is an amino acid that has been widely used in the food, agriculture and pharmaceutical industries. In order to screen a tyrosine phenol-lyase (TPL) with excellent catalytic performance for L-tyrosine production, TPL genes from Citrobacter freundii (CfTPL), Erwinia herbicola (EhTPL) and Rhodobacter...