Fish & Shellfish Immunology

Thu, 11/07/2019 - 03:24
Author(s): Hui Yang, Haoran Xiong, Kaihang Mi, Yingying Zhang, Xiaojun Zhang, Guohong ChenAbstractDue to the aquatic animal pathogens are numerous and specific, the pathogen invasion mechanisms are more complicated. The cell surface receptors play vital roles to understand these mechanisms. Syndecan is a cell surface protein and could function as a receptor involved bacteria and virus infections...
Wed, 11/06/2019 - 03:24
Author(s): Zhuoxing Zhang, Keke Han, Xiaoling Dai, Ruidong Zhang, Xueying Cao, Chao Zhang, Kaiqiang Wang, Xin Huang, Qian RenAbstractTwo lipopolysaccharides (LPS) and β-1, 3-glucan binding protein (LGBP), designated as PcLGBP isoform1 and PcLGBP isoform2, respectively, were identified from Procambarus clarkii in this study. The full-length cDNA of PcLGBP isoform1 was 1308 bp containing an open...
Tue, 11/05/2019 - 03:25
Author(s): Hui Li, Guilan Di, Yi Zhang, Rongrong Xue, Jing Zhang, Junping LiangAbstractCadmium (Cd) is a nonessential metal that is a contaminant in aquatic ecosystems. Cd can accumulate in aquatic animals, leading to detrimental effects in tissues, and Cd exposure can induce immunotoxicity in fish. MicroRNAs (miRNAs) play critical roles in immune responses, yet the participation of miRNAs in Cd-...
Sun, 11/03/2019 - 02:24
Author(s): Ying Huang, Qian RenAbstractKruppel-like factors (KLFs) belong to a family of zinc finger-containing transcription factors that are widely present in eukaryotes. In the present study, a novel KLF from the giant river prawn Macrobrachium rosenbergii (designated as MrKLF) was successfully cloned and characterized. The full-length cDNA of MrKLF was 1799 bp with an open reading frame of...
Sun, 10/27/2019 - 03:32
Author(s): Qiying Wang, Hao Li, Kaimin Zhou, Xiang Qin, Qun Wang, Weiwei LiAbstractThe Rab family is the most significant subfamily of small GTP-binding proteins. These proteins have widespread intracellular localization and play an important role in many biological processes. Rab7 plays a crucial role in the innate immune system of crustaceans. In the present study, we cloned and characterized...
Sun, 10/27/2019 - 03:32
Author(s): Jinzhong Niu, Yu Huang, Xinchao Liu, Guoling Luo, Jufen Tang, Bei Wang, Yishan Lu, Jia Cai, Jichang JianAbstractGalectin-3 is a kind of β-galactoside-binding lectin involved in host defense against pathogen infection. However, the immune functions of fish galectin-3 remain poorly understood. In this study, the roles of a fish galectin-3 (OnGal-3) from Nile tilapia (Oreochromis...
Sat, 10/26/2019 - 03:31
Author(s): Pan Wu, Yonghe Gu, Rou Zhao, Yaxin Liu, Yanling Wang, Guozhong Lv, Zhenghai Li, Yajing BaoAbstractThe improvement of resistance diseases, antioxidant and nonspecific immunity responses for Darkbarbel catfish by dietary residual pomegranate was investigated. The number of pathogenic bacteria and cumulative mortality of Darkbarbel catfish under 15–45% groups were decreased compared with...
Sun, 10/20/2019 - 03:29
Author(s): Dewei Cheng, Hongxing Liu, Hongkuan Zhang, Tan Kar Soon, Ting Ye, Shengkang Li, Hongyu Ma, Huaiping ZhengAbstractHeat shock proteins (HSPs) are a family of conserved proteins that enhance stress resistance and protect cells from external damage. In the present study, the full-length HSP70 cDNA from the noble scallop Chlamys nobilis (designated CnHSP70) was first cloned and...
Sun, 10/20/2019 - 03:29
Author(s): Ehsan Ahmadifar, Mohsen Shahriari Moghadam, Mahmoud A.O. Dawood, Seyed Hossein HoseinifarAbstractThis study investigates the possible effects of using Lactobacillus fermentum (LF) and/or ferulic acid (FA) in common carp (Cyprinus carpio) on some immunological parameters as well as resistance against Aeromonas hydrophila. Four diets were prepared including control diet and three diets...
Sun, 10/20/2019 - 03:29
Author(s): Ramasamy Harikrishnan, Gunapathy Devi, Bilal Ahmad Paray, Mohammad K. Al-Sadoon, Seyed Hossein Hoseinifar, Elumalai GokulAbstractAnthracenedione is a derivative of anthraquinone aromatic organic natural pigments found in senna, aloe latex, rhubarb, cascara, lichens, and fungi having broad range of bioactivity, including anti-cancer, anti-inflammatory, anti-microbial, anti-fungal, anti-...
Tue, 10/15/2019 - 03:32
Author(s): Elumalai Preetham, Abdul Salam Rubeena, Baskaralingam Vaseeharan, Mukesh Kumar Chaurasia, Jesu Arockiaraj, Rolf Erik OlsenAbstractThe study is carried out to understand the antimicrobial and immunological response of a potential immune molecule lectin, MmLec isolated from haemolymph of Speckled shrimp, Metapenaeus monoceros. MmLec was purified using mannose coupled Sepharose CL-4B...
Sun, 10/13/2019 - 03:29
Author(s): Xiaogang Du, Dong Li, Yunkun Li, Jiayu Wu, Anqi Huang, Guixian Bu, Fengyan Meng, Fanli Kong, Xiaohan Cao, Xingfa Han, Xiaofu Pan, Wei fan, Shiyong Yang, Xianyin ZengAbstractMammal Toll-like receptor 5 (TLR5) can directly recognize bacterial flagellin and therefore to initiate the inflammatory signaling cascades and trigger body immune system to clear the “non-self” substances. In...
Sun, 10/13/2019 - 03:29
Author(s): Shijuan Shan, Rongrong Liu, Hanxiao Feng, Yonghuan Zhang, Fumiao Zhang, Cui Lv, Guiwen YangAbstractp65 is an important subunit of the transcription factor NF-κB in the regulation of immune response. In the present study, the p65 cDNA was identified from common carp (Cyprinus carpio L.) (named Ccp65). Phylogenetic analysis revealed that Ccp65 located in the same clade as piscine p65 and...
Sun, 10/13/2019 - 03:29
Author(s): Yu-Lei Chen, Keke Han, Xin Huang, Zhuoxing Zhang, Xihe Wan, Qian RenAbstractThe caspase is an essential module in the Drosophila immune deficiency (IMD) pathway, which plays a crucial role in countering pathogen infection. In this study, a gene named PcCaspase-3C was found in Procambarus clarkia with a full-length of 4684 bp, including a 1572 bp opening reading frame, which encoded a...
Sun, 10/13/2019 - 03:29
Author(s): Zhi-qiang Du, Bo Li, Xiu-li Shen, Kai Wang, Jie Du, Xiao-dong Yu, Jian-jun YuanAbstractThe main advantage of antimicrobial peptides (AMPs) used as the effectors in the innate immunity system of invertebrates is that the high specificity is not indispensable. And they play important roles in the systemic defenses against microbial invasion. In this study, a new full-length cDNA of the...
Sun, 10/13/2019 - 03:29
Author(s): Lingsheng Bao, Yuanhua Chen, Honghui Li, Jianshe Zhang, Ping Wu, Ke Ye, Honglian Ai, Wuying ChuAbstractGinkgo biloba leaf is widely used in traditional medicine in China. The present study aimed to illustrate the effects of dietary Ginkgo biloba leaf extract (GBLE) on growth performance and immune responses in common carp infected by Aeromonas hydrophila. Six different diets either not...
Sat, 10/05/2019 - 03:27
Author(s): Gao Xiaolong, Ke Caihuan, Zhang Mo, Li Xian, Wu Fucun, Liu YingAbstractThe effects of a diet containing the probiotic Bacillus amyloliquefaciens on the survival and growth of Haliotis discus hannai were evaluated by measuring growth and hematological parameters and the expression levels of nonspecific immune genes. In addition, the abalone's response to Vibrio parahaemolyticus...
Sat, 10/05/2019 - 03:27
Author(s): Dan-Dan Chen, Ji-Hong Li, Yuan-Yuan Yao, Yong-An ZhangAbstractAeromonas hydrophila is a pathogen that causes high mortality in the grass carp. The complement system, as a frontline defence of innate immunity, plays an important role in the immune response against pathogens. However, the immunity evasion mechanism of A. hydrophila against the complement system of grass carp remains...
Thu, 10/03/2019 - 03:28
Author(s): Pan Wu, Yuqiao Hu, Yanling Wang, Yuan Wu, Ning Li, Yuying Dong, Ying ZhangAbstractThe employment of traditional bait and medicament in freshwater aquaculture causes the frequent occurrence of environmental pollution and disease. Effluent collected after Rhodopseudomonas capsulatus-mediated wastewater treatment could be re-utilized as microbial feeds, medicament, and aquaculture water...
Mon, 09/30/2019 - 03:21
Author(s): Yuan Luo, Yun-Ni Zhang, Han Zhang, Hong-Bo Lv, Mei-Ling Zhang, Li-Qiao Chen, Zhen-Yu DuAbstractPeroxisome proliferator-activated receptor α (PPARα) plays critical physiological roles in energy metabolism, antioxidation and immunity of mammals, however, these functions have not been fully understood in fish. In the present study, Nile tilapia (Oreochromis niloticus) were fed with...
Sun, 09/29/2019 - 03:21
Author(s): Yu-Xuan Zhang, Jin-Xing Wang, Xian-Wei WangAbstractWhey acidic protein domain (WAPD) is a usual motif in crustaceans, and is found mainly in the immune-related proteins. In the present study, a protein containing three tandem WAPDs was identified in red swamp crayfish Procambarus clarkii and designated as PcTWD. This is the first report of a protein of such domain architecture in...
Sun, 09/29/2019 - 03:21
Author(s): Yulong Gong, Fan Yang, Junpeng Hu, Cui Liu, Haokun Liu, Dong Han, Junyan Jin, Yunxia Yang, Xiaoming Zhu, Jianhua Yi, Shouqi XieAbstractA 56-day growth trial was conducted to investigate the effects of dietary yeast hydrolysate on the growth performance, antioxidation, immune response and resistance against Aeromonas hydrophila in largemouth bass. Four experimental diets were prepared...
Sun, 09/29/2019 - 03:21
Author(s): Chao Peng, Dongchang Xie, Chao Zhao, Haidong Xu, Sigang Fan, Lulu Yan, Pengfei Wang, Lihua QiuAbstractAkirin, which are members of the NF-κB signaling pathway, play critical roles in regulating the expression of antimicrobial peptides. In the present study, the Akirin gene from Penaeus monodon was identified from a transcriptome database and designated as PmAkirin. The complete...
Sun, 09/29/2019 - 03:21
Author(s): Hengwei Deng, Wenfeng Zhang, Jingjing Li, Jinling Li, Lei Hu, Wenyan Yan, Shanshan Liu, Jianguo He, Shaoping WengAbstractA STAT gene from Scylla paramamosain, named SpSTAT, was cloned and characterized. The full length of SpSTAT mRNA contains a 5′untranslated region (UTR) of 238 bp, an open reading frame (ORF) of 2388 bp and a 3′ UTR of 326 bp. The SpSTAT protein contains four...
Sun, 09/29/2019 - 03:21
Author(s): Qian Feng, Yueqian Huang, Defu Yao, Chunhua Zhu, Shengkang Li, Hongyu Ma, Jude Juventus Aweya, Yueling ZhangAbstractProtein kinase CK2 (CK2) is a ubiquitous serine/threonine kinase with multiple cellular functions in vertebrates including apoptosis, differentiation, proliferation, survival, tumorigenesis, signal transduction, immune regulation and inflammation. In the current study,...
Sun, 09/29/2019 - 03:21
Author(s): Xiaoxu Liu, Weiye Li, Lihua Jiang, Zhenming Lü, Minhai Liu, Li Gong, Bingjian Liu, Liqin Liu, Xiaolong YinAbstractLong non-coding RNA refers to an RNA transcript of a non-coding protein with a sequence length greater than 200 bp. More and more reports indicated that lncRNA was involved in the regulation of gene expression as a signalling molecule, an inducing molecule, a leader...
Sun, 09/29/2019 - 03:21
Author(s): S.L. Edirisinghe, S.H.S. Dananjaya, Chamilani Nikapitiya, T.D. Liyanage, Kyoung-Ah Lee, Chulhong Oh, Do-Hyung Kang, Mahanama De ZoysaAbstractIn this study, we demonstrate the enhanced disease resistance and positive immunomodulation of novel pectin isolated from Spirulina maxima (SmP) in zebrafish model. Zebrafish larvae exposed to SmP had significantly (p < 0.05) higher cumulative...
Sun, 09/29/2019 - 03:21
Author(s): Ruixia Wang, Xiucai Hu, Aijun Lü, Rongrong Liu, Jingfeng Sun, Yeong Yik Sung, Yajiao SongAbstractSkin plays an important role in the innate immune responses of fish, particularly towards bacterial infection. To understand the molecular mechanism of mucosal immunity of fish during bacterial challenge, a de novo transcriptome assembly of crucian carp Carassius auratus skin upon Aeromonas...
Tue, 09/24/2019 - 03:27
Author(s): Doaa M. MokhtarAbstractThe changes in the ovarian stroma of the fish during their spawning season become it an excellent biological model for studies on cellular and vascular elements due to the intense tissue remodeling in fish occur naturally throughout this critical period. The present study aims to investigate the cellular and vascular components of the ovarian stroma of Redbelly...
Mon, 09/23/2019 - 03:21
Author(s): Dinglong Yang, Yijing Han, Lizhu Chen, Ruiwen Cao, Qing Wang, Zhijun Dong, Hui Liu, Xiaoli Zhang, Qianqian Zhang, Jianmin ZhaoAbstractBactericidal permeability-increasing protein (BPI) is an antimicrobial protein with potent endotoxin-neutralising activity and plays a crucial role in innate immunity against bacterial infection. In the present study, a bpi (designed as rpbpi) was...
Mon, 09/23/2019 - 03:21
Author(s): Xianyun Ren, Yunbin Zhang, Ping Liu, Jian LiAbstractThis study aimed to use isobaric tags (IBTs) to investigate the immune response of the hepatopancreas of Marsupenaeus japonicas infected with Vibrio parahaemolyticus or white spot syndrome virus (WSSV). Liquid chromatography-tandem mass spectrometry and protein sequencing identified 1005 proteins. Among them, 109 proteins were...
Sun, 09/22/2019 - 03:21
Author(s): Jing Li, Zhi-Bin Wu, Zhao Zhang, Ji-Wei Zha, Shen-Ye Qu, Xiao-Zhou Qi, Gao-Xue Wang, Fei LingAbstractNowadays, there is no suitable treatment for vibriosis in groupers. So an eco-efficient and environmentally friendly treatment is necessary for the grouper industry. Probiotic-feeding has been a promising strategy to control the bacterial pathogens in aquaculture. A new Bacillus...
Sun, 09/22/2019 - 03:21
Author(s): Fan-Bin Zhan, Kianann Tan, Xiaoran Song, Jiongying Yu, Wei-Min WangAbstractToll-like receptors (TLRs) are a category of pattern recognition receptors (PRRs), which recognize pathogen associated molecular patterns (PAMPs) and participate in the immune responses. We identified tlr5a, tlr5b, tlr9 and tlr21 from the genome of blunt snout bream (Megalobrama amblycephala). All four tlrs were...
Sun, 09/22/2019 - 03:21
Author(s): Mariana Maluli Marinho de Mello, Camila de Fátima Pereira de Faria, Fábio Sabbadin Zanuzzo, Elisabeth Criscuolo UrbinatiAbstractIn this study, we show that β-glucan can modulate cortisol release in fish. We simulated a common situation in aquaculture: the transport of fish followed by contact with an opportunistic pathogen and observed what effect glucan had on the immune and stress...
Sun, 09/22/2019 - 03:21
Author(s): Phennapa Promthale, Pattira Pongtippatee, Boonsirm Withyachumnarnkul, Kanokpan WongprasertAbstractFishmeal is the main source of protein in the shrimp feed industry and is normally derived from trash fish. As such, the production of fishmeal has an adverse effect on the marine environment by taking away small and juvenile fish, leading to depletion of marine species. There is a need...
Sun, 09/22/2019 - 03:21
Author(s): Jiquan Zhang, Yujie Liu, Yongzhao Zhou, Wenzheng Wang, Naike Su, Yuying SunAbstractTrehalose, a nonreducing disaccharide, is present in a wide variety of organisms and plays a key role in many organisms under different stress conditions. In the study, the full-length cDNA sequence encoding trehalose-6-phosphate synthase (EcTPS) was obtained from Exopalaemon carinicauda. The complete...
Sun, 09/22/2019 - 03:21
Author(s): Mengting Luo, Linwei Yang, Zi-ang Wang, Hongliang Zuo, Shaoping Weng, Jianguo He, Xiaopeng XuAbstractC-type lectins (CTLs) are a group of lectins with at least one carbohydrate recognition domain (CRD), the binding of which to carbohydrates requires the presence of calcium ions. CTLs generally function as pattern recognition receptors (PRRs), essentially participating in innate...
Sun, 09/22/2019 - 03:21
Author(s): Yuanxia ChengAbstractThis study aims to investigate the effects of Rhodiola rosea polysaccharide (RRP) on the growth performance and nonspecific immunity of red swamp crayfish Procambarus clarkia. RRP was prepared by hot water extraction and partly characterised by high-performance liquid chromatography and sugar composition analyses. Three diets supplemented with three different...
Sun, 09/22/2019 - 03:21
Author(s): Jianning Zhang, Yifei Zhang, Linbao Zhang, Qianyu Wei, Xiaoli Liu, Dinglong YangAbstractIn the present study, a sialic acid-binding lectin was cloned and characterized from Manila clam Ruditapes philippinarum (designed as RpSabl). The open reading frame of RpSabl encoded a polypeptide of 162 amino acids with a calculated molecular mass of 17.7 kDa. Analysis of the conserved domain...
Sun, 09/22/2019 - 03:21
Author(s): Jiajia Yu, Hongxia Wang, Xin Yue, Baozhong LiuAbstractMeretrix petechialis is an important commercial aquaculture species in China. During the clam culture period, mass mortality events often occurred due to the Vibrio infection. In this paper, M. petechialis were challenged with Vibrio parahaemolyticus immersion to simulate a natural infection, and the infection process were divided...
Sun, 09/22/2019 - 03:21
Author(s): Pan Wu, Wentao Mo, Yanling Wang, Yuan Wu, Ying Zhang, Zhaobo Chen, Ning LiAbstractThe employment of traditional bait and medicament in freshwater aquaculture causes the frequent occurrence of environmental pollution and disease. Effluent collected after Rubrivivax gelatinosus-mediated wastewater treatment could be re-utilized as microbial feeds, medicament, and aquaculture water to...
Sun, 09/22/2019 - 03:21
Author(s): Ronald Lulijwa, Andrea C. Alfaro, Fabrice Merien, Mark Burdass, Leonie Venter, Tim YoungAbstractWe investigated cellular functional and targeted immune cytokine responses of farmed Chinook salmon (Oncorhynchus tshawytscha) peripheral blood mononuclear cells (PBMCs) in vitro to LPS from Escherichia coli (E. coli) serotypes O111: B4 and O55: B5, and a phorbol ester phorbol 12-myristate...
Sun, 09/22/2019 - 03:21
Author(s): Werasan Kewcharoen, Prapansak SrisapoomeAbstractAcute hepatopancreatic necrosis disease (AHPND), a serious disease caused by some Vibrio spp., impacts the Pacific white shrimp industry worldwide, especially in Thailand. To effectively overcome this problem, efficacious probiotic candidates were isolated from shrimp farms near coastal areas. The isolated Bacillus probiotics were...
Sun, 09/22/2019 - 03:21
Author(s): Lizhu Tao, Xiaoyan Xu, Yuan Fang, Anqi Wang, Fenglin Zhou, Yubang Shen, Jiale LiAbstractGrass carp septicemia is a systemic inflammatory response that develops following a bacterial infection. The hyperinflammatory state develops could lead to septic shock and lethality. There is increasing evidence that microRNAs are involved in the regulation of the inflammatory response. In the...
Sun, 09/22/2019 - 03:21
Author(s): Jianning Zhang, Yifei Zhang, Lizhu Chen, Jianmin Yang, Qianyu Wei, Bowen Yang, Xiaoli Liu, Dinglong YangAbstractIn the study, two c-type lectins were identified and characterized from the manila clam Venerupis philippinarum (designed as VpClec-1 and VpClec-2, respectively). Multiple alignments and phylogenetic analysis strongly suggested that they were new members of the c-type lectin...
Sun, 09/22/2019 - 03:21
Author(s): Ming Li, Chunxia Ma, Peng Zhu, Yanhao Yang, Aiyingi Lei, Xiaohan Chen, Wanwen Liang, Ming Chen, Jianha Xiong, Chaozheng LiAbstractCrustin is an antimicrobial peptide (AMP) that plays a key role in the innate immunity of crustaceans. This study cloned a new crustin from Pacific white shrimp Litopenaeus vannamei, which we designated as LvCrustinB, using rapid amplification of cDNA ends (...
Sun, 09/22/2019 - 03:21
Author(s): Crystal Guluarte, Martha Reyes-Becerril, Daniel Gonzalez-Silvera, Alberto Cuesta, Carlos Angulo, María Ángeles EstebanAbstractThe aim of this study was to analyze the probiotic potential, fatty acid composition and immunostimulant activities of Kluyveromyces lactis M3 isolated from a hypersaline sediment. For this purpose, K. lactis M3 resistance to different pH, salinities and bile,...
Sun, 09/22/2019 - 03:21
Author(s): Man Zhang, Zhenguang Wang, Conggang Wang, Xiaowan Ma, Guangping Cheng, Ying QiaoAbstractNk-lysin is an effector protein of cytotoxic T lymphocytes and natural killer cells. It is known to possess anti-bacterial, anti-fungal, anti-viral, and anti-tumor activity. Here we describe five Nk-lysin genes (PbNkla, PbNklb, PbNklc, PbNkld, and PbNkle) from Pseudocrossocheilus bamaensis, a rare...
Sun, 09/22/2019 - 03:21
Author(s): Shuang Zhang, Chaozheng Li, Wei Wang, Chenggui Wang, Chengbo Sun, Siuming Chan, Lili ShiAbstractProtein inhibitor of activated STAT (PIAS) plays a critical role in the feedback modulation of the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway as a negative regulator in mammals and Drosophila, but the function of PIAS in crustaceans is still...