Food Analytical Methods

Tue, 10/20/2020 - 02:02
Salting-out assisted liquid-liquid extraction (SALLE) coupled to dispersive liquid-liquid microextraction (DLLME) was developed as an efficient extraction method for purification and preconcentration of bisphenol A and six analogs (B, E, F, S, BADGE, BFDGE) in canned coffee drinks. Ultra-performance liquid chromatography-tandem mass spectrometry was applied for the determination of the analytes....
Sun, 10/18/2020 - 02:01
This work proposes, for the first time, a method based on homogenous liquid-liquid microextraction with switchable hydrophilicity solvent as extraction phase (SHS-HLLME) for the determination of food-packaging contaminants including dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP), benzyl butyl phthalate (BBP), di-n-octyl phthalate (DNOP), 4-octylphenol (4-OP), 4-...
Sun, 10/18/2020 - 02:01
Fruits contain nutrients, minerals, trace elements, vitamins, and phytochemicals that are important in terms of human health. When fruits are consumed in a balanced diet, they have a positive effect with regard to reducing disease risk. As a result of heavy metal contamination of the environment, they can be a potential source of toxic elements so it is important to determine the toxic, essential...
Fri, 10/16/2020 - 02:01
This paper describes a novel strategy based on fluorescence energy transfer (FRET) with green synthesis of carbon quantum dots (CQDs) and gold nanoparticles (GNPs) for the detection of one of the most pathogenic bacteria, Staphylococcus aureus (S. aureus). Novel green CQDs with blue color emission and high quantum yield were synthesized from olive leaves via the hydrothermal method. The...
Fri, 10/16/2020 - 02:01
Melamine adulteration is a fraudulent practice to increase the protein content of food in some areas of the world. In 2008, many babies in China suffered from serious illness and death because of melamine-tainted powdered infant formula. The issue prompted researchers to develop methods on detecting melamine in milk powder. Optical spectroscopy technologies have been proven to be effective in...
Tue, 10/13/2020 - 02:01
Single-stranded DNA aptamers that specifically bind to sulfaquinoxaline (SQX) were selected by a graphene oxide–based SEL EX (GO-SELEX) technique assisted by a non-immobilizing TAMRA-labeled oligonucleotide library. After 8 rounds of selection against SQX, two aptamer candidates (i.e., SBA 1 and SBA 2) were obtained. Furthermore, the two full-length aptamers were truncated to obtain the aptamers...
Mon, 10/12/2020 - 02:02
Alkaline phosphatase (ALP) is an active enzyme present in the raw milk. Due to its unique heat-sensitive properties, it is employed as an indicator to evaluate efficiency of the pasteurization treatment. In the present study, a method comprising of HPLC with fluorescence detector was developed for the quantification of ALP enzyme activity in the pasteurized milk. The method is based on the...
Wed, 09/30/2020 - 02:01
Nowadays, the overuse of antibiotics in cow breeding is a common issue worldwide. Real-time monitoring is critical for antibiotic control of dairy products. Herein, we report a rapid detection method for the duplex detection of two beta-lactam antibiotics (cephalexin and ampicillin) in milk powder using lateral flow (LF) combined with surface-enhanced Raman spectroscopy (SERS). The duplex...
Sat, 09/26/2020 - 02:01
A novel quantitative photothermal-sensing immunochromatographic assay (PT-ICA) with high sensitivity was developed for the detection of bisphenol A (BPA). Gold acid chloride trihydrate (HAuCl4·3H2O) was first reduced by citric acid trisodium salt (C6H5Na3O7) to form Au nanoparticles and adsorbed on the surface of black phosphorus nanosheets to form a black phosphorus-Au (BP-Au) complex. The...
Sun, 09/13/2020 - 02:02
The use of antimicrobial agents in livestock breeding is inevitable for prophylactic and treatment purposes, in order to maintain health and improve production of food products. However, this practice resulted in development of community resistance to antimicrobials due to residues found in livestock products. Milk is a key nutritional product that’s widely consumed for its high nutritional value...
Sun, 09/13/2020 - 02:01
Snack samples like potato crisp, fried potato, popcorn, and Cheetos have been known as carbohydrate- and protein-rich food. These tasty foods have high potential to contain acrylamide. Genotoxic and carcinogenic effect of acrylamide on human health cause to specific focus for measuring of acrylamide in food samples (Esposito et al. Food Chem Toxicol 108:249–256, 2017). In the present research,...
Sat, 09/12/2020 - 02:02
A double signal enhanced surface plasmon resonance (SPR) biosensor system was developed, which involved graphene-based magnetic dispersion solid-phase extraction (MDSPE) and carboxylated graphene oxide (GO-COOH) materials as amplifying elements in order to achieve real-time determinations of estradiol (E2), diethylstilboestrol (DES), and bisphenol A (BPA) via an indirect competition method. In...
Thu, 09/10/2020 - 02:02
In the food supply chain, quality control has a very important role in maintaining customer confidence. In the EU, food safety aspects are strictly regulated; however, composition requirements and standard control methods are generally undefined. The rapidly increasing wild boar population has a growing market share in venison or game meat production. Several methods have been described for...
Wed, 09/09/2020 - 02:02
A dispersive liquid–liquid micro-extraction method was combined with high-performance liquid chromatography to rapidly and efficiently separate and detect organophosphorus pesticide residues in coarse cereals. In order to select the best conditions, the extraction conditions, including the type and volume of the extraction agent, the type and volume of the dispersant, extraction time, pH, and the...
Fri, 09/04/2020 - 02:01
Yeast extract (YE) is tremendously used as a flavor enhancer in foods because of its composition. However, different types of YE have different aromatic profiles. This study focuses on the thermal treatment of glutathione-enriched yeast extract (GSH-YE) at different temperatures with the addition of Maillard precursors to investigate any change in its volatile organic profile. Headspace gas...
Fri, 09/04/2020 - 02:01
Chloramphenicol is a broad-spectrum antibiotic that is administered to cattle, fish, poultry, and honeybees among other animals. Consumption of food from such sources leads to non-regenerative anaemia, aplastic anaemia, and bone marrow suppression in humans. It is therefore a pressing necessity to detect chloramphenicol in food items through a convenient, accurate, and rapid method. The proposed...
Fri, 09/04/2020 - 02:01
The novelty of this research work lies in a simple, fast, and plausible analysis of acrylamide in food samples accomplished using an iOS gadgets–based digital imaging colorimeter (iOS gadgets–based DIC). This method is based on fluorescence color measurements obtained from digital images of derivatized acrylamide solution extracted from snack, seasoning, and refreshment food samples (fried banana...
Tue, 09/01/2020 - 02:01
In this study, mAb-5A7 and mAb-1D7, two highly specific monoclonal antibodies against tylosin (TYL), were prepared by using hapten TYL-carboxymethoxylamine hemihydrochloride (TYL-CMO), and they showed IC50 values of 13.84 ng/mL and 13.01 ng/mL and LODs of 1.21 and 0.98 ng/mL, respectively, in indirect competitive enzyme-linked immunosorbent assay. An immunochromatographic assay based on quantum...
Sat, 08/29/2020 - 02:02
Ochratoxins are possible human carcinogens. The aim of this study is to develop a rapid and sensitive competitive immunofluorescent analysis for determination of ochratoxin A (OTA) on the base of immobilized polyclonal antibody against ochratoxin and immobilized F(ab′)2 fragment on magnetic nanoparticles (MNPs). F(ab′)2 fragment of anti-OTA antibody was obtained by pepsin hydrolysis of polyclonal...
Thu, 08/06/2020 - 02:01
Fusarium-damaged kernels (FDK) contain a wide spectrum of mycotoxins, affecting the quality and safety of wheat used as food and feed. At present, traditional methods to detect FDK are time-consuming and laborious. Therefore, we propose herein a new spectral classification index (NSCI) method that can provide simple and low-cost FDK detection by analysing spectra in the wavelength range 350–2500 ...
Fri, 07/24/2020 - 02:01
Pentachlorophenol (PCP), commonly used as a wood preservative, pesticide, and fungicide, is the most toxic of all chlorophenols and can accumulate in living organisms. It is of great significance to construct a sensitive method for the detection of PCP in real samples. In this study, a simple, novel, and sensitive electrochemical sensor for pentachlorophenol (PCP) was constructed by modifying...
Fri, 07/17/2020 - 02:01
Whey protein-based supplements are one of the most consumed products to improve performance, and they are, generally, recognized as being safe. However, recent studies indicated different types of adulterations in these products, including the presence of diuretics and stimulants, allowing the possibility of contamination by other substances with pharmacological activities, such as anabolic...
Mon, 07/13/2020 - 02:01
Bentonite-coated activated carbon (BCAC) as an effective adsorbent can absorb soluble impurities in food to improve the detection sensitivity of Rti-LAMP assay. In the present study, BCAC was optimally prepared and characterized by SEM-EDS, FTIR, Raman spectrum, and pHPZC. The adsorptive kinetics and capacities of BCAC for four representatives, including indigo carmine (IC), tryptophan, thiamine...
Fri, 07/10/2020 - 02:01
Peptide nucleic acids (PNAs) are DNA/RNA analogs in which sugar-phosphate backbone is replaced by N-2-aminoethylglycine repeating units. Since PNA contains a neutral skeleton, there is no electrostatic repulsion, resulting in significant stability of its hybrid structure with complementary oligonucleotides. At present, PNA has taken the place of DNA probe in many studies. There are several...
Thu, 07/09/2020 - 02:01
Recently, surface-enhanced Raman spectroscopy (SERS) has attracted extensive concern of domestic and international researchers owing to the enormous signal amplification and unparalleled detection capability. However, developing the multifunctional SERS substrate materials through one facile way still remains a great challenge today. Herein, a novel dual functional sponge-based three-dimensional...
Wed, 07/08/2020 - 02:01
Unexpected contaminations of unauthorized genetically modified microorganisms (GMM) harbouring antimicrobial resistance (AMR) genes in food and feed enzymes, additives and flavourings commercialized on the European market have recently alerted the competent authorities regarding the food and feed safety. At the control level, we have therefore proposed a PCR-based strategy as first line screening...
Sun, 07/05/2020 - 02:01
Vibrio parahaemolyticus (V. parahaemolyticus) is one of the most common and widely distributed food-borne bacteria. Thus, it is necessary to establish a rapid, specific, and sensitive detection approach for V. parahaemolyticus. The strategy of combining immunomagnetic separation method (IMS) and polymerase chain reaction (PCR) measure was established successfully for V. parahaemolyticus detection...
Sat, 07/04/2020 - 02:01
QuEChERS combined with ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) was developed to analyze multi-class mycotoxins including aflatoxins, ochratoxins, and Alternaria toxins in apples and tomatoes. Using apples and tomatoes as the research models, the method performance had been evaluated, showing great convenience, high sensitivity, and good reliability. The...
Wed, 07/01/2020 - 02:01
In this study, a combination of modified quick easy cheap effective rugged and safe extraction (QuEChERS) and dispersive liquid–liquid microextraction (DLLME) has been proposed for the extraction and preconcentration of multiclass pesticide residues from yogurt samples prior to their determination by fast gas chromatography–mass spectrometry. Factors influencing DLLME efficiency, including type...
Sun, 06/28/2020 - 02:01
In this work an HPLC/hydrophilic interaction liquid chromatography (HILIC) system for intact glucosinolates was optimized using a weak anion exchange resin to remove carboxylic phenolics that absorbed at the same wavelength (229 nm) as glucosinolates, by selectively protonating contaminating phenolics with pKas from 2.98 to 4 while leaving the second OH of the sulfate of the thioglycosidic...
Wed, 06/24/2020 - 02:01
Animal feed is a potential route for contaminants like mycotoxins to enter into the human food chain. Hence, close monitoring is fundamental and should be performed with adequate analytical methods. In this study, a commercial ELISA kit for aflatoxin B1 detection in feed corn samples, RIDASCREEN® Aflatoxin B1 30/15, was assessed by the evaluation of some performance parameters. Basically, the...
Sun, 06/21/2020 - 02:01
Photothermal effect of nanomaterial has been applied to the establishment of rapid detection method such as test strip. Previously, the detector for the photothermal test strip method mainly was a bulky thermal imaging camera or an infrared-sensing temperature sensor of which the detection data obtained is unstable due to its susceptibility to environmental changes. Herein, a resistive...
Thu, 06/18/2020 - 02:01
A selective and reliable method was developed and validated for the determination of 14 lipophilic pesticides in raw propolis by gas chromatography–tandem mass spectrometry (GC-MS/MS) analysis. A test portion of milled sample was extracted with n-hexane followed by back extraction with acetonitrile and cleanup using EMR-Lipid and Florisil column adsorption. Matrix-matched standards were used to...
Wed, 06/17/2020 - 02:01
Due to high toxicity to mammals and hazard to the ecosystem, parathion has been banned or limited for use in many countries. It is essential to ensure food safety by parathion suspecting. On-site testing, as a critical component of the food chain safe system, plays a major role in the protection of food safety and human health. In this study, based on monoclonal antibody (Mab), a rapid and...
Thu, 05/28/2020 - 03:25
In this study, a specific monoclonal antibody (mAb) against Alternaria mycotoxin tenuazonic acid (TeA) was prepared and a sensitive indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for the detection of TeA was developed. Tenuazonic acid coupled with carboxymethoxylamine hemihydrochloride (TeA-CMO) was conjugated to keyhole limpet hemocyanin (KLH) as an immunogen for Balb/c mice...
Mon, 05/25/2020 - 03:22
A new method for the quantification of four aflatoxins (AFB1, AFB2, AFG1, and AFG2) in baby food samples is described herein. In this method, the extraction/cleanup step was performed by using multi-walled carbon nanotubes as a sorbent material in a dispersive solid phase procedure. In sequence, these mycotoxins were quantified via liquid chromatographic coupled with tandem mass spectrometry. The...
Wed, 05/20/2020 - 03:22
Pesticide residues are detected in food items globally at levels that may cause health problems. Paper-based analytical devices have emerged as an alternative low-cost and field-based tools for determining target analytes including the pesticide residues. However, the paper devices with colorimetric detection system are limited by lower sensitivity due to their inability to record wavelength...
Tue, 05/12/2020 - 03:22
We developed a self-priming compartmentalization (SPC) micro-device made of polymethyl methacrylate (PMMA) and integrated a loop-mediated isothermal amplification (LAMP) system for performing multiplex visual detection. The approach had a high throughput of identification of selectable marker gene (SMG) (phosphomannose isomerase (PMI) gene, hygromycin B phosphotransferase (HPT) gene, noglycoside...
Sun, 05/10/2020 - 03:23
In order to monitor and respond rapidly to outbreaks of food-borne pathogens, establishing rapid and sensitive detection methods is becoming increasingly urgent. In this work, through the synthesis of copper-based metal-organic framework nanoparticles (Cu-MOF NPs) and functionalized with aptamers, we developed a colorimetric protocol to detect Escherichia coli. In a typical experimental procedure...
Sun, 05/03/2020 - 03:22
Loop-mediated isothermal amplification (LAMP) had been employed as a powerful tool to facilitate genetic tests for various food pathogens, as it is easy to perform. Recently, various methods of detecting the LAMP amplicon were developed. In this study, we improved two LAMP assays by combining LAMP with chromatographic flow dipstick (LFD) assays for Salmonella (targeting phoP and invA,...
Sun, 05/03/2020 - 03:22
In this work, we present a magnetic bead–based immunosensorss for the determination of Escherichia coli O26 in milk. The assay uses commercial Dynabeads® for the capture of the target bacteria from 6-h enrichment broth and horseradish peroxidase–labelled polyclonal antibodies and detection of the peroxidase activity by chronoamperometry. Bovine seroalbumin was used as blocking reagent for...
Sun, 04/19/2020 - 03:20
This preliminary study describes the use of high resolution and accuracy mass spectrometry techniques combined with new generation chemical software products for detecting and identifying contaminants in food commodities. As a first step, the extracts of routine target analysis samples (obtained in our official laboratory responsible for food residues control) were acquired and processed with...
Sun, 04/19/2020 - 03:20
In this paper, the technique of continuous sample drop flow microextraction (CSDF-ME) is developed by the addition of a narrow-necked conical vessel. In the developed technique, an organic solvent denser than water is used for the extraction of organophosphorus pesticides (OPPs) from fruit juice and river water, followed by analysis with GC-MS. Eight milliliters of the sample solution is pumped...
Sun, 04/19/2020 - 03:20
Avermectin (AVM) has been widely used in agriculture and animal husbandry for the treatment of broad spectrum parasitic diseases, but it has a harmful effect on human body through food chain. In this paper, on the basis of anti-AVM specific antibody, expanded mesoporous silica-encapsulated gold nanoparticles (EMSN-AuNPs)-based enzyme-linked immunosorbent assay (ELISA) for the detection of AVM was...
Tue, 03/31/2020 - 03:16
Twenty-two mycotoxins were investigated in bread and biscuits samples (n = 115) purchased from Tunisia. Deoxynivalenol (DON), enniatin B (ENB), ENB1, and zearalenone (ZEA) occurred in the samples with ENB detected in all the samples. Whole bread was the most contaminated matrix showing the highest amounts of DON (25.2 ng/g), ENB (10.7 ng/g), and ENB1 (5.4 ng/g). However, biscuits showed a maximum...
Tue, 03/31/2020 - 03:16
In the present study, an elevated temperature homogeneous liquid extraction coupled with ionic liquid‑based dispersive liquid–liquid microextraction was developed to extract five carbamate pesticides (pirimicarb, carbaryl, methiocarb, aldicarb, and carbofuran) from packed fruit juice samples. The method consisted of a two-step procedure in which cyclohexylamine was used in order to extract the...
Tue, 03/31/2020 - 03:16
The non-destructive method for detection of fungal contamination in peach fruit using hyperspectral imaging was evaluated. Growth characteristics of three major spoilage fungi in peach fruit during decay were estimated. Three quantitative prediction models were then constructed to forecast the microbial content from the HSI datasets. The prediction of fungal contamination on the fruit was...
Tue, 03/31/2020 - 03:16
Quinolone is a family of widely prescript antibiotics in veterinary medicine. Their excessive use can be causative of quinolone residues in foodstuffs of animal origin. Therefore, a magneto immunofluorescence assay in microwell plate has been developed and validated to screen quinolone in bovine milk. The assay is based on an indirect competitive method where the quinolone in the milk sample...
Thu, 03/26/2020 - 03:16
Reliable and sensitive detection of Staphylococcal enterotoxin B (SEB) is important to prevent potential human health risk from foods contaminated by SEB. Here, a triple-channel assay was developed for ultrasensitive detection of SEB. Based on the optical characteristics of tetramers composed of gold nanoparticles (AuNPs) and upconversion nanoparticles (UCNPs), the SEB amounts in food products...
Tue, 03/24/2020 - 03:25
Due to the legislative shortcomings, Atlantic salmon often shares the common name of San Wen Yu (Chinese ideogram 三文鱼) with other salmonids in China, therefore concealing the differences with the rest salmonids in price, nutrition, and even food safety aspect, and also paving the way for species adulteration. To help prevent this episode, the present study aimed to develop novel loop-mediated...

Pages