Food Analytical Methods

Tue, 07/06/2021 - 02:01
The development of detection method for aflatoxin gene cluster in Aspergillus flavus and Aspergillus parasiticusis is an effective approach to prevent the widespread of aflatoxins, which are considered as extremely, toxic substances. Herein, a hairpin DNA-assisted fluorescence probe switch based on Förster resonant energy transfer (FRET) was designed. In this work, the two terminals of hairpin...
Sat, 06/26/2021 - 02:01
A novel and sensitive chromatography method with modified Quick Polar Pesticide (QuPPe) sample preparation was validated for determination of eight high polar pesticides in cherries by liquid chromatography-triple quadrupole mass spectrometry (LC-MS/MS). The validation data executed the conditions of the European SANTE/12682/2019 Guidance Document. Good linearity (R2 > 0.99) was observed, and...
Sat, 06/26/2021 - 02:01
Herein an electrochemiluminescence (ECL) sensor based on graphene aerogel (GA) for detecting metronidazole (MTZ) was proposed. The ECL behavior of the Ru(bpy)32+-doped silica nanoparticles (SiO2@Ru(bpy)32+ NPs) was investigated with MTZ as the coreactant. Quantitative detection of MTZ was realized as MTZ could effectively enhance the ECL signal of the SiO2@Ru(bpy)32+ NPs. A wide linear range of 2...
Sat, 06/26/2021 - 02:01
Assessing and monitoring the growth and response of bacteria to antibiotics is of crucial importance in research laboratories, as well as in food, environment, medical, and pharmaceutical industrial applications. In this study, Escherichia coli was chosen as the model microorganism to evaluate its response (e.g., growth) to a commercial antibiotic—tetracycline. Thus, the objective of this work...
Fri, 06/25/2021 - 02:01
An efficient sample preparation method, dispersive solid-phase microextraction–dispersive liquid–liquid microextraction, coupled to gas chromatography–flame ionization detector was developed for the extraction, preconcentration, and determination of six pesticides (ametryn, penconazole, oxadiazon, diniconazole, tebuconazole, and fenoxaprop-P-ethyl) in vegetable and fruit juice samples. In this...
Thu, 06/10/2021 - 02:01
The long-term accumulation of pesticide residues brought a huge threat to healthy lives of animals and humans. However, traditional detection methods for pesticide residues required expensive equipment and tedious operation. An electrochemiluminescence (ECL) aptasensor based on nano-antimony tin oxide and titanium dioxide composites (ATO@TiO2) and silver nanoparticles (AgNPs) for rapid detection...
Thu, 06/10/2021 - 02:01
The neomycin residues in milk could pose potential hazard for human health, and now, immunoassays are widely used for on-site screening of neomycin and other food contaminate residues. In this paper, the neomycin B was conjugated with carrier protein as immunogens, and a highly specific antibody was produced. Based on the produced antibody, an enzyme-linked immunosorbent assay (ELISA) and a new...
Wed, 06/09/2021 - 02:01
Ochratoxin A (OTA) is considered as one of the most hazardous mycotoxins commonly occurring in grains. Thus, the monitoring of OTA is extremely significant. Herein, a synergistic strategy to detect OTA in grains based on the combination of DNA tetrahedron-structured aptamer (DTA) and hybridization chain reaction (HCR) was proposed. Briefly, DTA was assembled on the gold electrode. In the presence...
Sun, 06/06/2021 - 02:01
A novel sensitive method for simultaneous determination of 19 antibiotics and 19 anthelmintics in edible fungi using QuEChERS-UHPLC-MS/MS method was established. For every 10.0 g sample, 20 mL of acetonitrile with 2% acetic acid and 1.2 g sodium acetate were used for extraction, followed by 6.0 g anhydrous MgSO4 for dehydration, and 50 mg anhydrous MgSO4+10 mg PSA for purification. The optimized...
Tue, 06/01/2021 - 02:01
Abstract Vegetables are one of the most important nitrate sources of human diary diet. Establishing of fast and accurate in situ nitrate monitoring approaches that could be used in the plant growth process and vegetable markets is essential. Incorporating the unique feature of N − O asymmetric stretch absorption in the mid-infrared region (1500–1200 cm−1), portable attenuated total reflectance–...
Thu, 05/20/2021 - 02:02
Using a recently developed genetically modified microorganisms (GMM) detection strategy, unexpected contaminations of unauthorized GMM in commercialized microbial fermentation products have been reported. A first-line real-time PCR screening analysis was initially performed to determine the presence of key targets frequently found in genetically modified (GM) bacteria. A second-line real-time PCR...
Fri, 05/14/2021 - 02:01
In this study, a multi-component analytical method for the detection of pesticide residues in paprika by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) was developed and validated. The sample preparation is based on an extraction step with acetonitrile followed by a cleanup step using dispersive solid-phase extraction. Mass...
Tue, 05/11/2021 - 02:01
The tuna fish processing industry produces more than 50% of waste and by-products (scales, head, viscera, etc.) that are usually discarded or used to produce low added value products such as fish meals. However, these waste materials still contain molecules essential for the human diet, such as essential metals, vitamins, omega-3 fatty acids, amino acids, and proteins of high nutritional value....
Thu, 05/06/2021 - 02:01
In recent years, food safety issues have become more serious, which caused extensive study on various methods for detecting food hazards. Gold nanoparticles (AuNPs) have drawn significant attention in nanoscience for the construction of biosensors because of their good biocompatibility, excellent quenching effect, unique surface plasmon resonance (SPR) properties, and high surface-to-volume ratio...
Wed, 05/05/2021 - 02:01
In this paper, the efficient magnetic dummy molecular imprinted polymers were firstly synthesized by dummy template (raffinose). The dummy molecularly imprinted layer was polymerized on the surface of Fe3O4 nanoparticles modified with NH2 group. The micromorphology and chemical composites of prepared polymers were detailed characterized. Their adsorption and desorption conditions including...
Wed, 05/05/2021 - 02:01
3-Monochloropropane-1,2-diol (3-MCPD) monoesters are a form of 3-MCPD esters known as potential food contaminant that may occur in a variety of food samples. A dispersive solid-phase extraction (dSPE) was developed to provide clean-up and pre-concentration in the determination of 1-palmitoyl-3-MCPD and 1-oleoyl-3-MCPD prior to gas chromatography-flame ionization detector analysis (GC-FID)....
Tue, 05/04/2021 - 02:01
A cold-induced-homogeneous liquid–liquid extraction method combined with deep eutectic solvent-based dispersive liquid–liquid microextraction has been done to extract several endocrine disrupting compounds (phenol, p-chlorophenol, p-nitrophenol, octylphenol, methyl paraben, ethyl paraben, butyl paraben, and bisphenol A) and hydroxymethylfurfural from honey samples before their determination by...
Wed, 04/14/2021 - 02:01
Injudicious and ever-increasing use of pesticides has made consumers more aware and critical about quality control and quality assurance in fruits and vegetables. Presence of pesticide residues in food commodities is one of the most indispensable parameters in quality control and quality assurance. To meet out the demand of acceptable or no residues in crops, different agencies viz. Codex...
Sun, 04/11/2021 - 02:01
Abstract Recently, consumption of collagen-based foodstuffs has grown significantly, especially collagen hydrolysate, used as a dietary supplement. Information available on the content of essential and toxic elements in these products, however, is incipient. This study provides a novel assessment of the mineral profile (Al, As, Ca, Cd, Co, Cr, Fe, K, Mg, Mn, Na, P, S, V, and Zn) of collagen...
Wed, 04/07/2021 - 02:01
A simple, rapid, and efficient liquid chromatography tandem mass spectrometry (LC–MS/MS) method, operated in electrospray ionization (ESI) and quadrupole linear ion trap modes, has been developed for the identification and structural characterization of zearalenone (ZEN) in corn oil. Samples (5 g) were extracted with acetonitrile/water (80:20, v/v). After centrifugation and dilution, the extracts...
Sun, 03/14/2021 - 03:01
Fumonisins are mycotoxins and corn has been considered the foodstuff presenting the highest contamination levels by these compounds. In recent years, the presence of hidden fumonisins in corn and corn products has aroused the interest of public health authorities. The aim of this study was to optimize and validate a simple and fast analytical method for the determination of free and hidden...
Sat, 03/13/2021 - 02:01
As a novel detection method of CuSO4, terahertz (THz) spectroscopy detection has great application prospects, but the terahertz detection with the tablet method has low sensitivity, and with the metamaterials has complicated manufacture. This work has proposed a high-sensitivity pesticide THz spectroscopic detection method with a novel metal grating. The metal grating was composed of a circular...
Sat, 03/13/2021 - 02:01
A β-cyclodextrin functionalised graphene oxide-grafted silica (β-CD@GO/SiO2) sorbent was synthesised and used as a solid-phase extraction (SPE) sorbent for the extraction of trace polycyclic aromatic hydrocarbons (PAHs) from fried food extract. The prepared β-CD@GO/SiO2 was characterised using Fourier transform infrared spectrometry (FT-IR) and elemental analysis (EA). Compared with graphene...
Thu, 03/11/2021 - 02:01
Food is intricate, and food quality and safety are indispensable elements. With the rising public demands for safe and nutritious food products, a range of innovative, interdisciplinary, and multidimensional concepts have emerged in the last few decades. Nanozymes, apart from their therapeutic efficacies, are gaining increasing interest in safety and quality monitoring in the agri-food sector....
Sun, 03/07/2021 - 02:01
Tomato is well-known to be one of the most cultivated and consumed vegetables worldwide and frequently contain pesticide residues. Therefore, a simple multiresidue method was established and validated to determine 129 pesticides and metabolites in tomato samples using a modified acetate QuEChERS without cleanup for sample preparation and determination by ultrahigh-performance liquid...
Fri, 02/05/2021 - 02:01
A simple, rapid, and accurate method for determination of selenocystine (SeCys2), methyl selenocysteine (MeSeCys), selenourea (SeUr), selenite (Se(IV)), selenotmethionine (SeMet), and selenate (Se(VI)) in grain crops was developed, based on the coupling of ion-pair reversed-phase high-performance liquid chromatography (IP-RP-HPLC) and ultraviolet irradiation hydride generation atomic fluorescence...
Tue, 01/26/2021 - 02:01
Indonesian coconut sugar (n = 109 samples) from different local producers was analyzed over a 2-year period. A mean δ13C value of − 25.6‰ ± 0.4‰ was determined for unadulterated (pure) coconut sugar. Seasonal climatic differences of up to ± 0.4‰ increase the standard deviations of coconut sugar (− 25.6‰ ± 0.8‰). Up to 5% w/v of cane sugar is often added during production for seeding purposes...
Thu, 01/21/2021 - 02:01
The aim of this study was to develop an analytical method, which separates selected chlorinated polycyclic aromatic hydrocarbons (Cl-PAHs) from fat, and fat-free or vegetable matrices. The method contains extraction-, cleanup-, and quantification steps. Integration of automated analysis actions, as in extraction and cleanup, should enhance the reproducibility, precision, and efficiency of the...
Thu, 01/21/2021 - 02:01
In the present work, a new and efficient sample treatment method based on the combination of stirring-dependent magnetic dispersive solid phase microextraction and ferrofluid-based dispersive liquid–liquid microextraction has been developed for the extraction of some pyrethroid pesticides (bifenthrin, phenothrin, tetramethrin, cyhalothrin, permethrin, and cypermethrin) from fruit juices prior to...
Thu, 01/21/2021 - 02:01
The illicit burn practices on farms promote food contamination that is harmful and must be controlled by improved analytical methods. Polycyclic aromatic hydrocarbons (PAHs), which have high toxic and carcinogenic potential, can contaminate foods, mainly through fire exposure. Considering the toxicity of PAHs, development of sensitive, simple, and solvent-free methods to analyze PAHs in foods is...
Wed, 01/13/2021 - 02:01
A simple methodology for the simultaneous determination of 67 pesticides, 25 veterinary drugs, and aflatoxin M1 in raw milk was developed and validated. After evaluating different combinations of sample preparation protocols, an extraction with acetonitrile:water (2:1) with QuEChERS citrate buffer salts and a combination of RP-C18 and anhydrous magnesium sulfate for the dispersive clean-up, using...
Sat, 01/09/2021 - 02:01
Hemp is one of the most complete plants for industrial and consumer purposes. In the food industry, the main hemp product is the seed oil, which contains a wide spectrum of compounds such as amino acids and fatty acids that are essential for human health. Given its growing consumption, systematic studies were undertaken for the quantification of toxic elements (As, Cd, and Pb) in hemp products,...
Fri, 01/08/2021 - 02:01
Minerals such as calcium, magnesium, and potassium which are available in the Ajwa dates are known to play vital roles in the regulation of blood pressure due to their interaction with the arterial resistance. The purpose of this study was to investigate the level of various ions in these date seeds and their correlation to the cardiovascular parameters. The powder was subjected to the electron...
Thu, 01/07/2021 - 02:01
Vegetables are potential vehicles for the transmission of parasites. Helminth eggs have been detected in produce in various countries around the world, with high prevalence rates. However, there is no standard validated method or ISO to the isolation, concentration, and identification of helminth eggs in salad vegetables, which makes comparison difficult since each study utilized different...
Wed, 01/06/2021 - 02:01
To detect enrofloxacin (ENR) and ciprofloxacin (CIP) residues in fishery foods, a computer simulation was used to predict and analyze the binding sites existing aptamer specific to ENR and CIP. A new aptamer (SEQ.3) that can recognize ENR and CIP was screened and evaluated, and the dissociation constants (Kd) of SEQ.3 from ENR and CIP were determined to be 210.955 nM and 887.49 nM which are...
Wed, 01/06/2021 - 02:01
In this study, a real-time loop-mediated isothermal amplification (Rti-LAMP) combined with immunomagnetic beads separation (IMS) and ethidium bromide monoazide (EMA) treatment was developed for the rapid and ultrasensitive detection of viable Escherichia coli (E. coli) O157:H7 in milk without enrichment. Polyclonal antibodies against E. coli O157:H7 flagellum and specific immunomagnetic beads (...
Tue, 01/05/2021 - 02:01
Tiamulin is a semi-synthetic derivative of the natural antibiotic pleuromutilin and is widely used as a veterinary drug for swine. Herein, we report the development of a sensitive and reliable method for determining 8α-hydroxymutilin as a marker residue for tiamulin in swine tissue using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The method consists of sample extraction with...
Sun, 01/03/2021 - 02:01
Pollution of foods with chloropropanol fatty acid esters is a growing international food safety issue, especially contamination with 3-monochloropropane-1, 2-diol (3-MCPD) esters. Traditional GC/MS method using phenylboronic acid (PBA) as a derivatization reagent has been proven to be high selective. However, the disadvantage of this method is that excessive amounts of phenylboronic acid causes...
Sun, 01/03/2021 - 02:01
In recent years, there was increasing concerns on food safety issues associated with fluorescent whitening agents from package materials. Here, we reported the development of a facile and reliable method suitable for high-throughput quantification and identification of two common fluorescent whitening agents (FWA 184 and FWA 367) in cereal products (wheat and rice flour), platformed by high-...
Tue, 11/24/2020 - 02:01
Polyaniline-modified magnetic halloysite nanotube composites (MHNTs@PANI) were evaluated for the first time as an efficient adsorbent for the magnetic micro-solid-phase extraction of polycyclic aromatic hydrocarbons (PAHs) from beer samples prior to gas chromatography-mass spectrometric detection. MHNTs@PANI were prepared from naturally available material halloysite by coating magnetic HNTs with...
Tue, 11/24/2020 - 02:01
The combination of ultra-high-performance liquid chromatography (UHPLC) with time-of-flight mass spectrometry (ToF-MS) is one of the most powerful analytical tools in terms of selectivity, sensitivity and speed. However, due to the gap on regulation and guidelines concerning the validation criteria for the use of ToF-MS methodology not only for screening but also for confirmatory and...
Wed, 11/18/2020 - 02:01
Tortoise shell is not only an armor, but also an important structure for storing nutrients for tortoise. The shell from one tortoise strain, Chinemys reevesii (CR), has long been used as a traditional Chinese medicine (TCM). Nowadays, massive CR shells are also processed into healthy food in Asian countries. Despite large-scale culture of CR in nowadays, there still exist massive counterfeit CR...
Fri, 11/13/2020 - 02:01
Macrolides are antimicrobials widely used in dairy farming, classified as critically important for human and animal health, and a priority for actions against antimicrobial resistance. Infant formula is used as a substitute for breast milk and should be safe to support child growth and development. In this context, the aim of this study was to validate an analytical method for macrolide analysis...
Wed, 11/11/2020 - 02:01
Vibrio cholerae is an important food-borne pathogenic bacterium that causes human disease, resulting in economic losses worldwide. Rapid diagnosis of cholera infection is important to avoid potential spread of disease. In this study, recombinase-aided amplification (RAA) primers labeled with biotin and fluorescein isothiocyanate (FITC) at 5′ end were prepared for the ctxA pathogenic gene of V....
Mon, 11/09/2020 - 02:01
A magnetic dispersive solid-phase microextraction procedure (MDμ-SPE) was developed to extract organophosphorus pesticides (OPP), including ethion, chlorpyrifos, diazinon, malathion, and fenitrothion, from vegetable samples before their analysis with gas chromatography/mass spectroscopy (GC/MS). Magnetic graphene@Fe3O4@SiO2@TiO2 nanocomposites were synthesized as a unique sorbent using the sol-...
Tue, 10/27/2020 - 02:01
Bovine β-casein is a highly polymorphic protein, with A1 and A2 representing the most frequent variants identified in Western cattle breeds. Depending on the presence of histidine or proline at position 67 of the sequence, β-casein variants can be grouped in two families: those within the A1 family (A1-like), possessing histidine, and those within the A2 family (A2-like), with proline. Upon...
Sat, 10/24/2020 - 02:02
In this paper, using molybdenum disulfide doped with gold nanoclusters (Au NCs@MoS2) as the substrate, an aflatoxin aptamer sensor was constructed utilizing iron porphyrin organic porous material modified with streptavidin and loaded with gold nano-bipyramids (SA-Au NBPs@ porous aromatic framework (PAF)-40-Fe) as a label. The catalyzed reduction current of hydrogen peroxide (H2O2) by Au NBPs@PAF-...
Thu, 10/22/2020 - 02:01
The microbiological contamination of food causes concern for public health due to the pathogenic action of microorganisms and the increasing of antimicrobial resistance observed in bacterial strains. This work considers the use of fluorescence spectroscopy to study the effects of the presence of microorganisms in the chicken’s meat and therefore, how the detection of this presence can help on...
Tue, 10/20/2020 - 02:02
Salting-out assisted liquid-liquid extraction (SALLE) coupled to dispersive liquid-liquid microextraction (DLLME) was developed as an efficient extraction method for purification and preconcentration of bisphenol A and six analogs (B, E, F, S, BADGE, BFDGE) in canned coffee drinks. Ultra-performance liquid chromatography-tandem mass spectrometry was applied for the determination of the analytes....
Sun, 10/18/2020 - 02:01
This work proposes, for the first time, a method based on homogenous liquid-liquid microextraction with switchable hydrophilicity solvent as extraction phase (SHS-HLLME) for the determination of food-packaging contaminants including dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP), benzyl butyl phthalate (BBP), di-n-octyl phthalate (DNOP), 4-octylphenol (4-OP), 4-...

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