Food Analytical Methods

Wed, 04/14/2021 - 02:01
Injudicious and ever-increasing use of pesticides has made consumers more aware and critical about quality control and quality assurance in fruits and vegetables. Presence of pesticide residues in food commodities is one of the most indispensable parameters in quality control and quality assurance. To meet out the demand of acceptable or no residues in crops, different agencies viz. Codex...
Sun, 04/11/2021 - 02:01
Abstract Recently, consumption of collagen-based foodstuffs has grown significantly, especially collagen hydrolysate, used as a dietary supplement. Information available on the content of essential and toxic elements in these products, however, is incipient. This study provides a novel assessment of the mineral profile (Al, As, Ca, Cd, Co, Cr, Fe, K, Mg, Mn, Na, P, S, V, and Zn) of collagen...
Wed, 04/07/2021 - 02:01
A simple, rapid, and efficient liquid chromatography tandem mass spectrometry (LC–MS/MS) method, operated in electrospray ionization (ESI) and quadrupole linear ion trap modes, has been developed for the identification and structural characterization of zearalenone (ZEN) in corn oil. Samples (5 g) were extracted with acetonitrile/water (80:20, v/v). After centrifugation and dilution, the extracts...
Sun, 03/14/2021 - 03:01
Fumonisins are mycotoxins and corn has been considered the foodstuff presenting the highest contamination levels by these compounds. In recent years, the presence of hidden fumonisins in corn and corn products has aroused the interest of public health authorities. The aim of this study was to optimize and validate a simple and fast analytical method for the determination of free and hidden...
Sat, 03/13/2021 - 02:01
As a novel detection method of CuSO4, terahertz (THz) spectroscopy detection has great application prospects, but the terahertz detection with the tablet method has low sensitivity, and with the metamaterials has complicated manufacture. This work has proposed a high-sensitivity pesticide THz spectroscopic detection method with a novel metal grating. The metal grating was composed of a circular...
Sat, 03/13/2021 - 02:01
A β-cyclodextrin functionalised graphene oxide-grafted silica (β-CD@GO/SiO2) sorbent was synthesised and used as a solid-phase extraction (SPE) sorbent for the extraction of trace polycyclic aromatic hydrocarbons (PAHs) from fried food extract. The prepared β-CD@GO/SiO2 was characterised using Fourier transform infrared spectrometry (FT-IR) and elemental analysis (EA). Compared with graphene...
Thu, 03/11/2021 - 02:01
Food is intricate, and food quality and safety are indispensable elements. With the rising public demands for safe and nutritious food products, a range of innovative, interdisciplinary, and multidimensional concepts have emerged in the last few decades. Nanozymes, apart from their therapeutic efficacies, are gaining increasing interest in safety and quality monitoring in the agri-food sector....
Sun, 03/07/2021 - 02:01
Tomato is well-known to be one of the most cultivated and consumed vegetables worldwide and frequently contain pesticide residues. Therefore, a simple multiresidue method was established and validated to determine 129 pesticides and metabolites in tomato samples using a modified acetate QuEChERS without cleanup for sample preparation and determination by ultrahigh-performance liquid...
Fri, 02/05/2021 - 02:01
A simple, rapid, and accurate method for determination of selenocystine (SeCys2), methyl selenocysteine (MeSeCys), selenourea (SeUr), selenite (Se(IV)), selenotmethionine (SeMet), and selenate (Se(VI)) in grain crops was developed, based on the coupling of ion-pair reversed-phase high-performance liquid chromatography (IP-RP-HPLC) and ultraviolet irradiation hydride generation atomic fluorescence...
Tue, 01/26/2021 - 02:01
Indonesian coconut sugar (n = 109 samples) from different local producers was analyzed over a 2-year period. A mean δ13C value of − 25.6‰ ± 0.4‰ was determined for unadulterated (pure) coconut sugar. Seasonal climatic differences of up to ± 0.4‰ increase the standard deviations of coconut sugar (− 25.6‰ ± 0.8‰). Up to 5% w/v of cane sugar is often added during production for seeding purposes...
Thu, 01/21/2021 - 02:01
The aim of this study was to develop an analytical method, which separates selected chlorinated polycyclic aromatic hydrocarbons (Cl-PAHs) from fat, and fat-free or vegetable matrices. The method contains extraction-, cleanup-, and quantification steps. Integration of automated analysis actions, as in extraction and cleanup, should enhance the reproducibility, precision, and efficiency of the...
Thu, 01/21/2021 - 02:01
In the present work, a new and efficient sample treatment method based on the combination of stirring-dependent magnetic dispersive solid phase microextraction and ferrofluid-based dispersive liquid–liquid microextraction has been developed for the extraction of some pyrethroid pesticides (bifenthrin, phenothrin, tetramethrin, cyhalothrin, permethrin, and cypermethrin) from fruit juices prior to...
Thu, 01/21/2021 - 02:01
The illicit burn practices on farms promote food contamination that is harmful and must be controlled by improved analytical methods. Polycyclic aromatic hydrocarbons (PAHs), which have high toxic and carcinogenic potential, can contaminate foods, mainly through fire exposure. Considering the toxicity of PAHs, development of sensitive, simple, and solvent-free methods to analyze PAHs in foods is...
Wed, 01/13/2021 - 02:01
A simple methodology for the simultaneous determination of 67 pesticides, 25 veterinary drugs, and aflatoxin M1 in raw milk was developed and validated. After evaluating different combinations of sample preparation protocols, an extraction with acetonitrile:water (2:1) with QuEChERS citrate buffer salts and a combination of RP-C18 and anhydrous magnesium sulfate for the dispersive clean-up, using...
Sat, 01/09/2021 - 02:01
Hemp is one of the most complete plants for industrial and consumer purposes. In the food industry, the main hemp product is the seed oil, which contains a wide spectrum of compounds such as amino acids and fatty acids that are essential for human health. Given its growing consumption, systematic studies were undertaken for the quantification of toxic elements (As, Cd, and Pb) in hemp products,...
Fri, 01/08/2021 - 02:01
Minerals such as calcium, magnesium, and potassium which are available in the Ajwa dates are known to play vital roles in the regulation of blood pressure due to their interaction with the arterial resistance. The purpose of this study was to investigate the level of various ions in these date seeds and their correlation to the cardiovascular parameters. The powder was subjected to the electron...
Thu, 01/07/2021 - 02:01
Vegetables are potential vehicles for the transmission of parasites. Helminth eggs have been detected in produce in various countries around the world, with high prevalence rates. However, there is no standard validated method or ISO to the isolation, concentration, and identification of helminth eggs in salad vegetables, which makes comparison difficult since each study utilized different...
Wed, 01/06/2021 - 02:01
To detect enrofloxacin (ENR) and ciprofloxacin (CIP) residues in fishery foods, a computer simulation was used to predict and analyze the binding sites existing aptamer specific to ENR and CIP. A new aptamer (SEQ.3) that can recognize ENR and CIP was screened and evaluated, and the dissociation constants (Kd) of SEQ.3 from ENR and CIP were determined to be 210.955 nM and 887.49 nM which are...
Wed, 01/06/2021 - 02:01
In this study, a real-time loop-mediated isothermal amplification (Rti-LAMP) combined with immunomagnetic beads separation (IMS) and ethidium bromide monoazide (EMA) treatment was developed for the rapid and ultrasensitive detection of viable Escherichia coli (E. coli) O157:H7 in milk without enrichment. Polyclonal antibodies against E. coli O157:H7 flagellum and specific immunomagnetic beads (...
Tue, 01/05/2021 - 02:01
Tiamulin is a semi-synthetic derivative of the natural antibiotic pleuromutilin and is widely used as a veterinary drug for swine. Herein, we report the development of a sensitive and reliable method for determining 8α-hydroxymutilin as a marker residue for tiamulin in swine tissue using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The method consists of sample extraction with...
Sun, 01/03/2021 - 02:01
Pollution of foods with chloropropanol fatty acid esters is a growing international food safety issue, especially contamination with 3-monochloropropane-1, 2-diol (3-MCPD) esters. Traditional GC/MS method using phenylboronic acid (PBA) as a derivatization reagent has been proven to be high selective. However, the disadvantage of this method is that excessive amounts of phenylboronic acid causes...
Sun, 01/03/2021 - 02:01
In recent years, there was increasing concerns on food safety issues associated with fluorescent whitening agents from package materials. Here, we reported the development of a facile and reliable method suitable for high-throughput quantification and identification of two common fluorescent whitening agents (FWA 184 and FWA 367) in cereal products (wheat and rice flour), platformed by high-...
Tue, 11/24/2020 - 02:01
Polyaniline-modified magnetic halloysite nanotube composites (MHNTs@PANI) were evaluated for the first time as an efficient adsorbent for the magnetic micro-solid-phase extraction of polycyclic aromatic hydrocarbons (PAHs) from beer samples prior to gas chromatography-mass spectrometric detection. MHNTs@PANI were prepared from naturally available material halloysite by coating magnetic HNTs with...
Tue, 11/24/2020 - 02:01
The combination of ultra-high-performance liquid chromatography (UHPLC) with time-of-flight mass spectrometry (ToF-MS) is one of the most powerful analytical tools in terms of selectivity, sensitivity and speed. However, due to the gap on regulation and guidelines concerning the validation criteria for the use of ToF-MS methodology not only for screening but also for confirmatory and...
Wed, 11/18/2020 - 02:01
Tortoise shell is not only an armor, but also an important structure for storing nutrients for tortoise. The shell from one tortoise strain, Chinemys reevesii (CR), has long been used as a traditional Chinese medicine (TCM). Nowadays, massive CR shells are also processed into healthy food in Asian countries. Despite large-scale culture of CR in nowadays, there still exist massive counterfeit CR...
Fri, 11/13/2020 - 02:01
Macrolides are antimicrobials widely used in dairy farming, classified as critically important for human and animal health, and a priority for actions against antimicrobial resistance. Infant formula is used as a substitute for breast milk and should be safe to support child growth and development. In this context, the aim of this study was to validate an analytical method for macrolide analysis...
Wed, 11/11/2020 - 02:01
Vibrio cholerae is an important food-borne pathogenic bacterium that causes human disease, resulting in economic losses worldwide. Rapid diagnosis of cholera infection is important to avoid potential spread of disease. In this study, recombinase-aided amplification (RAA) primers labeled with biotin and fluorescein isothiocyanate (FITC) at 5′ end were prepared for the ctxA pathogenic gene of V....
Mon, 11/09/2020 - 02:01
A magnetic dispersive solid-phase microextraction procedure (MDμ-SPE) was developed to extract organophosphorus pesticides (OPP), including ethion, chlorpyrifos, diazinon, malathion, and fenitrothion, from vegetable samples before their analysis with gas chromatography/mass spectroscopy (GC/MS). Magnetic graphene@Fe3O4@SiO2@TiO2 nanocomposites were synthesized as a unique sorbent using the sol-...
Tue, 10/27/2020 - 02:01
Bovine β-casein is a highly polymorphic protein, with A1 and A2 representing the most frequent variants identified in Western cattle breeds. Depending on the presence of histidine or proline at position 67 of the sequence, β-casein variants can be grouped in two families: those within the A1 family (A1-like), possessing histidine, and those within the A2 family (A2-like), with proline. Upon...
Sat, 10/24/2020 - 02:02
In this paper, using molybdenum disulfide doped with gold nanoclusters (Au NCs@MoS2) as the substrate, an aflatoxin aptamer sensor was constructed utilizing iron porphyrin organic porous material modified with streptavidin and loaded with gold nano-bipyramids (SA-Au NBPs@ porous aromatic framework (PAF)-40-Fe) as a label. The catalyzed reduction current of hydrogen peroxide (H2O2) by Au NBPs@PAF-...
Thu, 10/22/2020 - 02:01
The microbiological contamination of food causes concern for public health due to the pathogenic action of microorganisms and the increasing of antimicrobial resistance observed in bacterial strains. This work considers the use of fluorescence spectroscopy to study the effects of the presence of microorganisms in the chicken’s meat and therefore, how the detection of this presence can help on...
Tue, 10/20/2020 - 02:02
Salting-out assisted liquid-liquid extraction (SALLE) coupled to dispersive liquid-liquid microextraction (DLLME) was developed as an efficient extraction method for purification and preconcentration of bisphenol A and six analogs (B, E, F, S, BADGE, BFDGE) in canned coffee drinks. Ultra-performance liquid chromatography-tandem mass spectrometry was applied for the determination of the analytes....
Sun, 10/18/2020 - 02:01
This work proposes, for the first time, a method based on homogenous liquid-liquid microextraction with switchable hydrophilicity solvent as extraction phase (SHS-HLLME) for the determination of food-packaging contaminants including dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP), benzyl butyl phthalate (BBP), di-n-octyl phthalate (DNOP), 4-octylphenol (4-OP), 4-...
Sun, 10/18/2020 - 02:01
Fruits contain nutrients, minerals, trace elements, vitamins, and phytochemicals that are important in terms of human health. When fruits are consumed in a balanced diet, they have a positive effect with regard to reducing disease risk. As a result of heavy metal contamination of the environment, they can be a potential source of toxic elements so it is important to determine the toxic, essential...
Fri, 10/16/2020 - 02:01
This paper describes a novel strategy based on fluorescence energy transfer (FRET) with green synthesis of carbon quantum dots (CQDs) and gold nanoparticles (GNPs) for the detection of one of the most pathogenic bacteria, Staphylococcus aureus (S. aureus). Novel green CQDs with blue color emission and high quantum yield were synthesized from olive leaves via the hydrothermal method. The...
Fri, 10/16/2020 - 02:01
Melamine adulteration is a fraudulent practice to increase the protein content of food in some areas of the world. In 2008, many babies in China suffered from serious illness and death because of melamine-tainted powdered infant formula. The issue prompted researchers to develop methods on detecting melamine in milk powder. Optical spectroscopy technologies have been proven to be effective in...
Tue, 10/13/2020 - 02:01
Single-stranded DNA aptamers that specifically bind to sulfaquinoxaline (SQX) were selected by a graphene oxide–based SEL EX (GO-SELEX) technique assisted by a non-immobilizing TAMRA-labeled oligonucleotide library. After 8 rounds of selection against SQX, two aptamer candidates (i.e., SBA 1 and SBA 2) were obtained. Furthermore, the two full-length aptamers were truncated to obtain the aptamers...
Mon, 10/12/2020 - 02:02
Alkaline phosphatase (ALP) is an active enzyme present in the raw milk. Due to its unique heat-sensitive properties, it is employed as an indicator to evaluate efficiency of the pasteurization treatment. In the present study, a method comprising of HPLC with fluorescence detector was developed for the quantification of ALP enzyme activity in the pasteurized milk. The method is based on the...
Wed, 09/30/2020 - 02:01
Nowadays, the overuse of antibiotics in cow breeding is a common issue worldwide. Real-time monitoring is critical for antibiotic control of dairy products. Herein, we report a rapid detection method for the duplex detection of two beta-lactam antibiotics (cephalexin and ampicillin) in milk powder using lateral flow (LF) combined with surface-enhanced Raman spectroscopy (SERS). The duplex...
Sat, 09/26/2020 - 02:01
A novel quantitative photothermal-sensing immunochromatographic assay (PT-ICA) with high sensitivity was developed for the detection of bisphenol A (BPA). Gold acid chloride trihydrate (HAuCl4·3H2O) was first reduced by citric acid trisodium salt (C6H5Na3O7) to form Au nanoparticles and adsorbed on the surface of black phosphorus nanosheets to form a black phosphorus-Au (BP-Au) complex. The...
Sun, 09/13/2020 - 02:02
The use of antimicrobial agents in livestock breeding is inevitable for prophylactic and treatment purposes, in order to maintain health and improve production of food products. However, this practice resulted in development of community resistance to antimicrobials due to residues found in livestock products. Milk is a key nutritional product that’s widely consumed for its high nutritional value...
Sun, 09/13/2020 - 02:01
Snack samples like potato crisp, fried potato, popcorn, and Cheetos have been known as carbohydrate- and protein-rich food. These tasty foods have high potential to contain acrylamide. Genotoxic and carcinogenic effect of acrylamide on human health cause to specific focus for measuring of acrylamide in food samples (Esposito et al. Food Chem Toxicol 108:249–256, 2017). In the present research,...
Sat, 09/12/2020 - 02:02
A double signal enhanced surface plasmon resonance (SPR) biosensor system was developed, which involved graphene-based magnetic dispersion solid-phase extraction (MDSPE) and carboxylated graphene oxide (GO-COOH) materials as amplifying elements in order to achieve real-time determinations of estradiol (E2), diethylstilboestrol (DES), and bisphenol A (BPA) via an indirect competition method. In...
Thu, 09/10/2020 - 02:02
In the food supply chain, quality control has a very important role in maintaining customer confidence. In the EU, food safety aspects are strictly regulated; however, composition requirements and standard control methods are generally undefined. The rapidly increasing wild boar population has a growing market share in venison or game meat production. Several methods have been described for...
Wed, 09/09/2020 - 02:02
A dispersive liquid–liquid micro-extraction method was combined with high-performance liquid chromatography to rapidly and efficiently separate and detect organophosphorus pesticide residues in coarse cereals. In order to select the best conditions, the extraction conditions, including the type and volume of the extraction agent, the type and volume of the dispersant, extraction time, pH, and the...
Fri, 09/04/2020 - 02:01
Yeast extract (YE) is tremendously used as a flavor enhancer in foods because of its composition. However, different types of YE have different aromatic profiles. This study focuses on the thermal treatment of glutathione-enriched yeast extract (GSH-YE) at different temperatures with the addition of Maillard precursors to investigate any change in its volatile organic profile. Headspace gas...
Fri, 09/04/2020 - 02:01
Chloramphenicol is a broad-spectrum antibiotic that is administered to cattle, fish, poultry, and honeybees among other animals. Consumption of food from such sources leads to non-regenerative anaemia, aplastic anaemia, and bone marrow suppression in humans. It is therefore a pressing necessity to detect chloramphenicol in food items through a convenient, accurate, and rapid method. The proposed...
Fri, 09/04/2020 - 02:01
The novelty of this research work lies in a simple, fast, and plausible analysis of acrylamide in food samples accomplished using an iOS gadgets–based digital imaging colorimeter (iOS gadgets–based DIC). This method is based on fluorescence color measurements obtained from digital images of derivatized acrylamide solution extracted from snack, seasoning, and refreshment food samples (fried banana...
Tue, 09/01/2020 - 02:01
In this study, mAb-5A7 and mAb-1D7, two highly specific monoclonal antibodies against tylosin (TYL), were prepared by using hapten TYL-carboxymethoxylamine hemihydrochloride (TYL-CMO), and they showed IC50 values of 13.84 ng/mL and 13.01 ng/mL and LODs of 1.21 and 0.98 ng/mL, respectively, in indirect competitive enzyme-linked immunosorbent assay. An immunochromatographic assay based on quantum...
Sat, 08/29/2020 - 02:02
Ochratoxins are possible human carcinogens. The aim of this study is to develop a rapid and sensitive competitive immunofluorescent analysis for determination of ochratoxin A (OTA) on the base of immobilized polyclonal antibody against ochratoxin and immobilized F(ab′)2 fragment on magnetic nanoparticles (MNPs). F(ab′)2 fragment of anti-OTA antibody was obtained by pepsin hydrolysis of polyclonal...

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