Food and Agricultural Immunology

Tue, 08/04/2020 - 14:04
The aim of this study was to evaluate the influence of Lactobacillus reuteri B1/1 on the local intestinal immune response in the cecum of chickens experimentally infected by C. jejuni CCM6189. Day-old chicken were divided into four groups: control (C), L. reuteri B1/1 (LR), C. jejuni CCM6189 (CJ), and combined L. reuteri B1/1 + C. jejuni CCM6189 (LRCJ). Cecal samples were collected during...
Tue, 08/04/2020 - 14:04
Aflatoxin M1 (AFM1) in milk is a problem of great concern. Current methods of detection require large instruments and need specific test sites. Therefore, it is necessary to establish a fast, convenient, and accurate detection method for AFM1. We established a system based on fluorescent microspheres containing a Eu3+ chelate named AFM1-POCT. These components comprised the AFM1-POCT kits. After...
Thu, 07/30/2020 - 14:04
Lead (Pb) is a toxic metal that can accumulate in the body and negatively impact human health. In this study, we prepared a highly sensitive and specific monoclonal antibody, 2C3, that can recognize Pb(II) with an IC50 and linear range of 0.5 ng/mL and 0.08–1.86 ng/mL, respectively. Additionally, based on this antibody we developed a fluorescent test strip, which can be used for both qualitative...
Tue, 07/28/2020 - 14:04
The establishment of nucleic acid amplification technology and functional nucleic acid aptamers promote the development of detection techniques. In particular, the detection displayed promise for broader application, as well as improved specificity ad higher sensitivity when combined with immunoassays (IA). This review introduced the novel immuno-nucleic acid (INA) cooperative detection...
Tue, 07/28/2020 - 14:04
Ochratoxin A (OTA) is a highly toxic potential contaminant of most agricultural products, and OTA is difficult to remove in general food production processes. In this study, we immobilized polyvinyl pyrrolidone-coated carboxypeptidase A (CPA) into zeolitic imidazolate framework material to improve the ability for OTA degradation. In this method, carboxypeptidase A was dispersed well in the...
Tue, 07/28/2020 - 14:04
Maximum permissible levels of mycotoxins in baby food may be 1% of those in ordinary food. Therefore, highly sensitive methods of mycotoxin control are in demand. To detect such low amounts, expensive instrumental methods are commonly used. Advantages of immunochromatographic analyses are their low cost and simple sample preparation; however, their sensitivity needs to be increased to contend...
Tue, 07/28/2020 - 14:04
In this study, a high sensitivity and specificity monoclonal antibody (mAb) was developed based on the hydroxy and carboxy-termini of ochratoxin A (OTA). A fluorescent microsphere immunochromatographic test strip (FM-ICTS) was assembled for the rapid detection the OTA in rice flour. Some relevant parameters were optimized including the best concentration of coating antigen and optimal diluent...
Fri, 04/03/2020 - 03:19
In this study, we produced a monoclonal antibody (mAb) 2F7 against cadmium (Cd) with IC50 of 0.20 ng/mL. The cross-reactivity of mAb 2F7 with other eight metals was < 1%. A fluorescent immunoassay strip was developed for the determination of Cd in rice. The cut-off value by visual inspection of the test strip was 50 μg/kg, and the quantitative detection range determined by the strip reader was...
Tue, 02/11/2020 - 03:19
Food contamination with antibiotic residues has become a worldwide problem due to the heavy and improper use of antibiotics. The development of fast, high-throughput, easy, and economical screening methods for the detection of antibiotic residues is an important requirement as an alternative to the traditional assays. Immunoassays are widespread promising methods that can achieve the conditions...
Wed, 01/08/2020 - 03:21
It is long-cherished to develop rapid approaches for the identification and quantification of pesticide and its metabolites. Carbofuran (CBF) and its metabolite 3-hydroxy-carbofuran (3-OH-CBF) are highly toxic to humans and non-target organisms, which makes it mandatory for the detection of their residues in foods and the environment in many countries. Herein, a colloidal gold-strip assay based...
Tue, 12/03/2019 - 03:35
Acrylamide (AA) is an important product of the Maillard reaction. Studies have demonstrated that AA caused oxidative stress damage in liver, which in turn triggered an inflammatory response, and the activation of NOD-like receptor protein-3 (NLRP3) inflammasome played a key factor in regulating inflammation. Therefore, we hypothesized NLRP3 inflammasome activation was involved in AA-induced...
Sun, 09/22/2019 - 03:18
A rapid, ultra-sensitive and simple fluorescence immunoassay (FLISA) on CdSe/ZnS Quantum dots (QDs) for recognition pesticide triazophos have been established. In this assay, the immunoassay uses (CdSe/ZnS) Quantum dots (QDs) as a probe that carries monoclonal antibody (mAb). The free triazophos and OVA-haptens were competed for binding the mAb on the surface of the QDs. Therefore, the...
Sun, 09/22/2019 - 03:18
The hapten 1 which was formed by the reaction of 2-chloro-4,6-diamine-1,3,5-triazine (CAAT) with 6-aminocaproic acid, conjugated with keyhole limpet haemocyanin to prepare the immunogen. And coating antigen was prepared by the coupling of ovalbumin and hapten 2 which was synthesized by the reaction of CAAT and 3-mercapto propionic acid. Based on immunological and cell fusion technology, the anti-...
Sun, 09/22/2019 - 03:18
In this study, we used 2-(diethexyphosphoryl)acetic acid as a common template molecule and Fe3O4@SiO2 as support material to prepare a superparamagnetic core/shell molecular imprinting polymer (MIP), Fe3O4@SiO2@MIP, which has multiple recognition sites and increased adsorption capacity. Then, a biomimetic fluorescence immunoassay method was developed for the simultaneous determination of methyl...
Sun, 09/22/2019 - 03:18
Bayberries are important sources of phytochemicals. In this paper, the active substances of bayberry extract (BE) were investigated, and based on this, the inhibitory effects of BE on the six tested food-borne pathogens were futher evaluated. Finally, the main antibacterial components in the extracts were identified by UV-Vis absorption spectroscopy, UPLC-UV spectrum and UPLC-ESI-MS. The results...