Future Microbiology

Wed, 09/23/2020 - 08:02
We evaluated the efficacy of three ethanol-based handrubs against murine norovirus in a proposed clinical simulation test (prEN 17430).
Sat, 10/19/2019 - 03:17
Aim: To show that a strain of Aeromonas hydrophila became resistant to carbapenems by interspecies transfer of a plasmid using long-read sequencing. Material & methods: Whole genome sequencing of the four isolates was done using Illumina Hiseq, while the plasmid was reconstructed using the MinION sequencer. The resistome was identified with ResFinder. Results: Whole genome sequencing and long...
Fri, 08/30/2019 - 07:34
Aim: Investigation of the lateral flagellar (Laf) genes transcription by the quorum sensing (QS) regulators AphA and OpaR in Vibrio parahaemolyticus. Materials & methods: Regulation mechanisms were assessed by combined utilization of swarming motility assay, qPCR, LacZ fusion, EMSA and DNase I footprinting. Results: AphA and OpaR oppositely regulate swarming motility and Laf genes. At high...
Fri, 08/02/2019 - 04:26
Aim: Determination of the virulence regulatory network controlled by the ATP-dependent Lon protease in Salmonella enterica serovar Typhimurium. Materials & methods: The effect of Lon on S. Typhimurium virulence genes expression was investigated by RNA sequencing, and virulence-associated phenotypes between the wild-type and lon mutant were compared. Results:SPI-1, SPI-4, SPI-9 and flagellar...
Wed, 07/31/2019 - 22:07
Aim: The aim of this study is to formulate a new single nonselective pre-enrichment medium (ELSS) that can support the concurrent growth of four major foodborne pathogens containing E. coli O157: H7, L. monocytogenes, S. aureus and S. enterica serovar Entertidis to develop a multiplex TaqMan Real-time PCR (mRT-PCR). Methods: The mRT-PCR with a new pre-enrichment was carried out for simultaneous...
Thu, 07/04/2019 - 05:19
Aim: Among the alternative sigma factors of Listeria monocytogenes, σB controls the largest regulon. The aim of this study was to perform a comprehensive review of σB-regulated genes, and the functions they confer. Materials & methods: A systematic search of PubMed and Web of Knowledge was carried out to identify members of the σB regulon based on experimental evidence of σB-dependent...
Mon, 03/11/2019 - 08:50
Vibrio Parahaemolyticus infections caused by the pandemic clone have become a global public health issue. The pandemic clone includes over ten sequence types and 49 serotypes. Several markers such as toxRS/new, orf8 and genomic islands were considered specific for pandemic strains, but subsequent studies later confirmed a lack of specificity. Thus, identifying stable indicators for the pandemic...
Sun, 01/20/2019 - 21:54
Aim: We evaluated the accuracy of a commercial rapid immunochromatographic test (rapid test [RT]) for hepatitis A (HA) diagnosis and epidemiological studies. Materials & methods: The accuracy of a RT was evaluated in laboratory and in field conditions. Predictive modeling estimated the test performance in a hypothetical population. Results: The RT showed sensitivities of 66–86%, and...
Tue, 12/11/2018 - 06:37
Aim: The purpose of current study is to find out relationship between cas9 gene and antimicrobial resistance in Campylobacter jejuni NCTC11168. Materials & methods: The involvement of the cas9 gene in antimicrobial resistance of C. jejuni was determined by assessment of minimum inhibitory concentration, clustered regularly interspaced short palindromic repeats (CRISPR)-cas gene expression in...
Fri, 09/21/2018 - 06:16
Aim: This study aims to evaluate the genetic and population structure of Vibrio parahaemolyticus in the major coastal regions of China. Materials & methods: Multilocus sequence typing was performed. Results: Insertion of large sequence into recA happened in nearly 30 strains, which were untypeable by multilocus sequence typing. A collection of 307 V. parahaemolyticus isolates were typed into...
Mon, 06/18/2018 - 04:55
Prosthetic joint infection diagnosis is often difficult since biofilm-embedded microorganisms attach well to the prosthetic surfaces and resist their detection by conventional methods. DL-dithiothreitol has been described as a valid method for biofilm detachment on orthopedic devices. We report the case of an occasional detection of Listeria monocytogenes in a non immuno-compromised patient with...
Wed, 05/30/2018 - 22:40
Aim: Determination of the effects of BirA on transcription and virulence in enterohemorrhagic Escherichia coli (EHEC) O157:H7. Materials & methods: The effect of BirA on EHEC O157:H7 gene expression and phenotypes was assessed by RNA-seq combined with adherence, quantitative biofilm and survival assays. Results: Many genes associated with virulence, amino acid synthesis and transport, and...
Sun, 12/10/2017 - 21:33
Aim: To screen host proteins that interact with enterohemorrhagic Escherichia coli O157:H7 EspF. Materials & methods: Flow cytometry and high-throughput sequencing were used to screen interacting proteins. Molecular function, biological processes and Kyoto Encyclopedia of Genes and Genomes pathways were studied using the DAVID online tool. Glutathione S-transferase pull down and dot blotting...
Wed, 08/16/2017 - 22:14
, Ahead of Print. Aim: To characterize the roles of VvhA in host's acquired immune response to Vibrio vulnificus infection. Materials & methods: The recombinant VvhA fusion protein was used to immunize mice and the anti-VvhA polyclonal antibody was produced in vivo for prophylactic and therapeutic efficacy assay. The roles of VvhA in T helper (Th) cells differentiation were analyzed by vvhA-...
Thu, 08/10/2017 - 00:07
Aim: Acid is an important environmental condition encountered frequently by Salmonella enterica serovar Typhimurium during its pathogenesis, but the role of small-noncoding RNAs (sRNAs) in response to acid stress is poorly understood. Methods: We used RNA sequencing to explore acid-responsive sRNAs in S. Typhimurium. Results: It identified that 6S RNA encoded by the ssrS was significantly...
Mon, 06/12/2017 - 05:25
The opportunistic intracellular bacterial pathogen Listeria monocytogenes has in 30 years emerged as an exceptional bacterial model system in infection biology. Research on this bacterium has provided considerable insight into how pathogenic bacteria adapt to mammalian hosts, invade eukaryotic cells, move intracellularly, interfere with host cell functions and disseminate within tissues. It also...
Wed, 06/07/2017 - 22:11
Aim: To evaluate the efficacy of attenuated Salmonella Typhimurium (JOL912) as a live bacterial vaccine vector. Materials & methods: The JOL912 engineered to deliver HA1 protein from influenza A/Puerto Rico/8/1934 (H1N1; PR8) virus was coined as JOL1635 and further evaluated for immunogenicity and protective efficacy. Results: The JOL1635 stably harbored the HA1 gene within pMMP65 plasmid...
Fri, 12/16/2016 - 00:44
Enterotoxigenic Escherichia coli (ETEC) are a major cause of traveler's diarrhea and infant mortality in developing countries. Given the rise of antibiotic resistance worldwide, there is an urgent need for the development of new preventive strategies. Among them, a promising approach is the use of probiotics. Although many studies, mostly performed under piglet digestive conditions, have shown...
Thu, 11/10/2016 - 00:27
Aim: Cronobacter sakazakii sequence types 1, 4, 8 and 12 are associated with outbreaks of neonatal meningitis and necrotizing enterocolitis infections. However clonality results in strains which are indistinguishable using conventional methods. This study investigated the use of clustered regularly interspaced short palindromic repeats (CRISPR)–cas loci profiling for epidemiological...