Journal of Agricultural and Food Chemistry

Fri, 06/18/2021 - 04:02
DNA-based detection methods are highly promising for risk assessment in the food sector, such as tracing the existence of food allergens. However, due to the complexity of food matrices, cumbersome protocols are often needed to isolate the DNA components, which hinder the achievement of rapid and on-site detection. Herein, an instant and multiple DNA extraction method was developed based on the...
Fri, 06/18/2021 - 04:02
This study presents a comprehensive two-dimensional gas chromatography with negative chemical ionization quadrupole time-of-flight mass spectrometry (GC × GC–NCI–QTOF/MS) method, which allows for a precise chromatographic separation of short-chain chlorinated paraffins (SCCPs) and medium-chain chlorinated paraffins (MCCPs). A new reversed-phase column setup was used, which allows for more...
Thu, 06/17/2021 - 04:02
In this study, the rhodamine 6G hydrazide (R6GH) complex was synthesized to develop an “off–on” output platform for fluorescence and visual dual-mode analysis of lead(II) (Pb2+). The prepared R6GH complex using the heat to reflux reaction of rhodamine 6G (R6G) and hydrazine hydrate was characterized through FT-IR, MS, 1H NMR, and 13C NMR and demonstrated to have good fluorescence stability and...
Sun, 06/13/2021 - 04:02
A new microfluidic approach using hybrid magnetoliposomes (h-MLs) containing hydrophobic magnetic nanoparticles (Fe3O4@AuNPs-C12SH) and encapsulated N-acetylcysteine has been developed in this research to determine aminoglycoside antibiotic (AAG) residues in food using o-phthalaldehyde. Four AAGs, kanamycin, streptomycin, gentamicin, and neomycin, have been used as model analytes. The h-MLs have...
Fri, 06/11/2021 - 04:03
Existing methods for the analysis of pesticides in human breast milk involve multiple extraction steps requiring large sample and solvent volumes, which can be a major obstacle in large epidemiologic studies. Here, we developed a simple, low-volume method for extracting organophosphates, pyrethroids, carbamates, atrazine, and imidacloprid from 100 to 200 μL of human breast milk. Multiple...
Fri, 06/11/2021 - 04:03
Pollution related to pesticides has become a global problem due to their low utilization and non-targeting application, and nanotechnology has shown great potential in promoting sustainable agriculture. Nowadays, mesoporous silica-based nanomaterials have garnered immense attention for improving the efficacy and safety of pesticides due to their distinctive advantages of low toxicity, high...
Wed, 06/09/2021 - 04:04
Lanthanum (La), calcium (Ca), and cadmium (Cd) have similar physical and chemical properties because of their similar ionic radius. Although the interactions between La, Ca, and Cd have been frequently reported in plants, few studies have investigated the interactive effects of La and Ca on the growth and Cd accumulation in plants. Therefore, we investigated the interactive effects of La and Ca...
Tue, 06/08/2021 - 04:03
Sixteen metabolites, including seven C7-alkylated salicylaldehyde derivatives (1–7) and nine prenylated indole alkaloids (8–16), three of which are new, namely, asperglaucins A and B (1 and 2) and neoechinulin F (8), were separated from the endolichenic fungus Aspergillus chevalieri SQ-8. Asperglaucin A (1) represents an unusual phthalide-like derivative with a benzo[c]thiophen-1(3H)-one scaffold...
Sun, 06/06/2021 - 04:03
Here, we prepared a series of novel osthole-type ester derivatives modified in the lactone ring of osthole, which is isolated from Cnidium monnieri. The positions of H-3 and H-4 of the representative compound 4z were determined by a 1H–1H COSY spectrum. By opening the lactone ring of osthole, the double bonds at the C-3 and C-4 positions of diol 3 and esters 4a–4z, 4a′, and 4b′ were still...
Wed, 06/02/2021 - 04:04
Citrinin (CIT) is a scarcely studied mycotoxin within foodstuffs, so the biomonitoring of this toxin and its metabolite dihydrocitrinone (DH-CIT) in biological samples represents the main alternative to estimate the exposure. Hence, this study aimed to evaluate the presence of CIT and DH-CIT in 300 urine samples from Italian individuals in order to assess the exposure. Quantification was...
Mon, 05/31/2021 - 04:02
A dietary pesticide residue causes underestimated influences on body health. In this work, experimental mice were exposed to commonly used pesticides that cause insulin resistance, inflammation, and non-alcoholic fatty liver diseases. Alterations in intestinal flora were detected in the exposure groups. The abundance of the flora causing high endotoxin production was intensively increased and led...
Tue, 05/25/2021 - 04:03
An immunoassay is mostly employed for the direct detection of food contaminants, and a molecular assay for targeting nucleic acids employs amplification techniques for distinguishing genes. The integration of an immunoassay with nucleic acid amplification techniques inherits the direct and rapid performance of an immunoassay and the ultrasensitive merit of a molecular assay. Enthusiastic...
Tue, 05/25/2021 - 04:03
SARS-CoV-2, the coronavirus strain that initiated the COVID-19 pandemic, and its subsequent variants present challenges to vaccine development and treatment. As the coronavirus evades the host innate immune response at the initial stage of infection, the disease can have a long nonsymptomatic period. The uridylate-specific endoribonuclease Nsp15 processes the viral genome for replication and...
Mon, 05/24/2021 - 04:03
Cadmium (Cd) accumulation in agricultural soils is an increasingly serious problem, as plants absorb Cd, which inhibits their growth and development. Nonetheless, the molecular mechanisms underlying Cd detoxification and accumulation in wheat (Triticum aestivum L.) are unclear. Here, we isolated the U-box E3 ligase TaPUB1 from wheat and reported the functional characterization of TaPUB1 in Cd...
Sat, 05/22/2021 - 04:03
Given the relevance of organophosphorus pesticides (OPs) with food safety, it is highly urgent to develop sensitive and reliable sensors for OPs. However, most of the OP sensors are developed based on colorimetric and fluorescent techniques, which are limited to severe interference of color and fluorescence from pigments and organic acids in agricultural crops. Herein, we develop an inorganic...
Wed, 05/19/2021 - 04:05
Exploring novel p-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27, HPPD) inhibitors has become one of the most promising research directions in herbicide innovation. On the basis of our tremendous interest in exploiting more powerful HPPD inhibitors, we designed a family of benzyl-containing triketone-aminopyridines via a structure-based drug design (SBDD) strategy and then synthesized them....
Mon, 05/17/2021 - 04:02
Bioglycosylation is an efficient strategy to improve biological activities and physicochemical properties of natural compounds to develop structural modifications of drugs. In this study, an N555 residue was identified as a candidate for site-directed mutagenesis through sequence alignment with GTF180ΔN. Caffeic acid phenethyl ester (CAPE) was used as an acceptor substrate. Two generated mutants...
Tue, 05/04/2021 - 04:02
Emerging and fugitive contaminants (EFCs) can be introduced into the food chain through plants, particularly crop plants, and have threatened food safety and human health. The method for determination of volatile EFCs in plant tissues remains challenging. A new rapid, simple, precise, and accurate freeze–thaw–equilibration followed by head space (HS)–solid-phase microextraction (SPME) and gas...
Fri, 04/23/2021 - 04:02
According to known studies, numerous mycotoxins have been found simultaneously in foods and have a certain expansion toxicity, so the simultaneous detection of multiple mycotoxins is absolutely critical. In this article, multifunctional magnetic g-C3N4/Fe3O4 nanocomposites have been fabricated to employ as modified QuEChERS adsorbents. In addition, they were also used in conjunction with...
Fri, 04/23/2021 - 04:02
Macadamia nut contains important food allergens that potentially cause allergic reactions with severe adverse effects in infants and adults. Reliable and accurate detection of macadamia is critical to avoid allergic reactions. However, knowledge on macadamia allergen is scarce and a reliable detection method has not been reported, yet. In this study, an unbiased immunization and selection...
Thu, 04/22/2021 - 04:02
The non-toxic immunoassay for mycotoxins is being paid more attention due to its advantages of higher safety and cost savings by using anti-idiotype antibodies to substitute toxins. In this study, with tenuazonic acid (TeA), a kind of highly toxic Alternaria mycotoxin as the target, an enhanced non-toxic immunoassay was developed based on the ferritin-displayed anti-idiotypic nanobody-...
Sat, 04/17/2021 - 04:02
Acrylamide, a food processing contaminant with demonstrated genotoxicity, carcinogenicity, and reproductive toxicity, is largely present in numerous prominent and commonly consumed food products that are produced by thermal processing methods. Food regulatory bodies such as the U.S. Food and Drug Administration (U.S. FDA) and European Union Commission regulations have disseminated various...
Sat, 04/17/2021 - 04:02
Currently, there are more than 1000 varieties of synthetic sulfonamides universally used as antibiotics causing severe results of potential carcinogenicity and drug resistance for human health due to excessive residue of animal-derived food. A facile and novel approach for untargeted screening of sulfonamides (SAs) and metabolites was proposed based on magnetic solid-phase extraction–ultrahigh-...
Sat, 04/17/2021 - 04:02
Plant bacterial diseases can potentially damage agricultural products around the world, and few effective bactericides can manage these infections. Herein, to sequentially explore highly effective antibacterial alternatives, 1,2,3-triazole-tailored carbazoles were rationally fabricated. These compounds could suppress the growth of three main intractable pathogens including Xanthomonas oryzae pv...
Wed, 04/14/2021 - 04:02
A multiclass analytical method to determine pesticides and mycotoxins in beer-related matrices based on the dilute and shoot approach was optimized and validated according to the European guidelines SANTE/12682/2019 and EC/401/2006. Extraction used acidified acetonitrile at 1% (v/v) acetic acid, followed by horizontal shaking homogenization, centrifugation, freeze-out step for cleanup, another...
Mon, 04/12/2021 - 04:02
An omnipresent pathogenic bacterium, Pseudomonas aeruginosa (P. aeruginosa PAO1), is easy to contaminate environmental water or foods, causing daily food spoilage and infections. The biofilm-forming ability and bacterial resistance of P. aeruginosa PAO1 make it difficult to be eradicated by traditional bacteriostatic agents. In this work, we designed and synthesized a pyridinium-based pillar[5]...
Sat, 04/10/2021 - 04:02
Natural products are one of the resources for discovering novel fungicidal leads. As a natural fungicide, osthole was used as a coumarin-based lead compound for the development of novel fungicides. Here, a series of 3,4-dichloroisothiazole-containing 7-hydroxycoumarins were rationally designed, synthesized, and characterized by introducing a bioactive substructure, 3,4-dichloroisothiazole, into...
Thu, 04/01/2021 - 04:02
The current approaches remain insufficient for measuring chicken egg spoilage or present analytical limitations. This study aimed to complement the existing analyses and identify novel markers using liquid chromatography-high resolution mass spectrometry-based foodomics strategies. In the discovery set, comparative untargeted metabolomics was utilized to identify marker candidates in microbially...
Thu, 04/01/2021 - 04:02
An increasing number of studies have suggested that PAH contamination in dairy products demands high concern. This study established an efficient determination method for the European Union 15 + 1 PAHs and four PAH derivatives in dairy samples using a QuEChERS method coupled with GC-QqQ-MS. The optimized method obtained a recovery of 63.38–109.17% with a precision of 3.82–15.62%, and the limit of...
Wed, 03/31/2021 - 04:02
The carbon–silicon switch strategy has become a key technique for structural optimization of drugs to widen the chemical space, increase drug activity against targeted proteins, and generate novel and patentable lead compounds. Flubeneteram, targeting succinate dehydrogenase (SDH), is a promising fungicide candidate recently developed in China. We describe the synthesis of novel SDH inhibitors...
Sat, 03/27/2021 - 04:02
Bisphenol A (BPA) is a well-recognized endocrine disruptor, and considering its adverse effects its use in infant bottles has been banned in many countries. Growing concern on the use of BPA has led to its replacement with its analogues in numerous applications. Present is the first report determining the occurrence of seven bisphenols (BPs: BPA, BPAF, BPC, BPE, BPFL, BPS, and BPZ) in Indian...
Sat, 03/20/2021 - 04:02
Both overproduced reactive oxygen species/reactive nitrogen species and hyperglycemic conditions accompany a significant increase in protein glycation and nitration that contribute to the initiation and progression of diabetic complications and neuronal disorders. In this study, 19 compounds, including steroidal saponins, alkaloids, cerebroside, phenolic compounds, sterols, and nucleosides, were...
Thu, 03/18/2021 - 04:02
Bacillus cereus is a Gram-positive endospore-forming foodborne pathogen that causes lethal food poisoning and significant economic losses, usually through biofilm- and endospore-induced recurrent cross- and postprocessing contamination. Due to the lack of critical inhibitory targets and control strategies, B. cereus biofilm contamination is a problem that urgently needs a solution. In this study...
Wed, 03/17/2021 - 04:02
Food authenticity and safety are major public concerns due to the increasing number of food fraud cases. Meat fraud is an economically motivated practice of covertly replacing one type of meat with a cheaper alternative raising health, safety, and ethical concerns for consumers. In this study, we implement the MasSpec Pen technology for rapid and direct meat analysis and authentication. The...
Sun, 03/14/2021 - 05:02
Oxathiapiprolin is a novel chiral piperidine thiazole isooxazoline fungicide that contains a pair of enantiomers. An effective analytical method was established for the enantioselective detection of oxathiapiprolin in fruit, vegetable, and soil samples using ultraperformance liquid chromatography-tandem triple quadrupole mass spectrometry. The optimal enantioseparation was achieved on a Chiralpak...
Fri, 03/12/2021 - 04:02
Pyrrolizidine alkaloids (PAs) are a type of natural phytotoxin that contaminate food and feed and become an environmental health risk to humans and livestock. PAs exert toxicity that requires metabolic activation by cytochrome P450 (CYP) 3A, and case reports showed that fetuses are quite susceptible to PAs toxicity. The aim of this study was to explore the characteristics of developmental...
Thu, 03/11/2021 - 04:02
Plant diseases result in 20–40% of agricultural loss every year worldwide. Timely detection of plant diseases can effectively prevent the development and spread of diseases and ensure the agricultural yield. High-throughput and rapid methods are in great demand. This review investigates the advanced application of Raman spectroscopy (RS) and surface-enhanced Raman spectroscopy (SERS) in the...
Wed, 03/10/2021 - 04:02
Traditional fresh produce washing systems mainly rely on mechanical forces and usage of chlorine bleach solutions to aid in removal and sanitization of microorganisms attached on surfaces of fresh produce during washing processes. Frequent outbreaks of foodborne diseases from ready-to-eat produce indicate insufficient sanitization of the washing processes. Herein, we present a scalable...
Tue, 03/09/2021 - 04:02
Nutraceuticals have been gradually accepted as food ingredients that can offer health benefits and provide protection against several diseases. It is widely accepted due to potential nutritional benefits, safety, and therapeutic effects. Most nutraceuticals are vulnerable to the changes in the external environment, which leads to poor physical and chemical stability and absorption. Several...
Wed, 03/03/2021 - 04:02
Although the colorimetric methods can easily meet the demands of point-of-care and ease-of-use for antibiotic detection, they still face many challenges in the accuracy and stability of assay. Herein, a facile and stable colorimetric aptasensor is first developed for kanamycin residue detection based on the aptamer-enhanced peroxidase-mimicking activity of layered WS2 nanosheets. The...
Tue, 03/02/2021 - 04:01
Nowadays, a lot of pesticides have been used in the agriculture field due to the global demand for food production. Carbofuran (CF) is the most commonly used carbamate compound that is responsible for the highest toxicity to humans compared to any other pesticide used in agricultural settings. Thus, rapid, portable, and low-cost sensors are needed for the detection of CF in the environment and...
Tue, 03/02/2021 - 04:01
Development of simple, sensitive, and reliable fluorescence sensors for monitoring the residue, distribution, and variation of organophosphorus pesticides (OPs) in agricultural crops is highly urgent but remains challenging, which is ascribed to deprivation of an ideal fluorophore and ingenious detection strategy. Herein, we report the fabrication of cadmium telluride quantum dots (CdTe QDs) with...
Tue, 03/02/2021 - 04:01
Pesticide residues are a food safety concern. A good detection method is critical for rapid and accurate determination of pesticide metabolites in crops and studying metabolism. The pretreatment methods have mainly been ultrasonic extraction–solid-phase extraction and QuEChERS, while detection methods have been radio-chromatography, nuclear magnetic resonance, and mass spectrometry. This...
Fri, 02/26/2021 - 04:02
Legume proteins are widely used as food ingredients, but only some (soybean, lupin, and peanut) must be declared under consumer safety regulations to protect allergy sufferers. It is not yet mandatory to declare pea proteins as allergens even though they are predicted to be allergenic based on cross-reactivity in sensitized people. The processing of legume proteins can modify their allergenic...
Sun, 02/21/2021 - 04:02
Triglyceride (TG) is a class of neutral lipids, which functions as an energy storage depot and is important for cellular growth, metabolism, and function. The composition and content of TG molecular species are crucial factors for nutritional aspects in food chemistry and are directly associated with several diseases, including atherosclerosis, diabetes, obesity, stroke, etc. As a result of the...
Sat, 02/20/2021 - 04:02
Staphylococcal enterotoxins (SEs) are responsible for frequent food poisoning outbreaks worldwide. Specific identification of SEs is crucial for confirmation of food poisoning, tracking of the incriminated foods or food ingredients, and removal from the food chain. Here, we report on a new food testing protocol addressing the challenge of low abundance of SEs in contaminated food and high...
Tue, 02/16/2021 - 04:02
Prions cause transmissible and inevitably fatal neurological diseases in agriculturally important animals, including bovine spongiform encephalopathy in domestic cattle, scrapie in sheep and goats, and chronic wasting disease in cervids. Because animals are largely asymptomatic throughout the course of the disease, early detection of prion disease is important. Hamsters were peripherally (ip)...
Mon, 02/15/2021 - 04:02
Surface-enhanced Raman scattering (SERS) with fast and intuitive property has been extensively utilized in the field of food safety. Here, we demonstrated a novel noble metal–polymer hybrid film as a SERS substrate for food fungicide analysis. Benefiting from its transparency and flexibility, poly(dimethylsiloxane) (PDMS) film was chosen as a versatile supporting matrix to grow gold nanobushes (...
Sat, 02/13/2021 - 04:02
In this study, a rapid and reliable method based on ultrahigh-performance liquid chromatography coupled with Q Exactive HF-X mass spectrometry (UHPLC-QE/MS) was established for the simultaneous quantification and validation of acrylamide, 5-hydroxymethylfurfural, and 14 heterocyclic aromatic amines in thermally processed foods. With the optimization of the pretreatment method, all 16 hazardous...
Sat, 02/13/2021 - 04:02
Four novel chimeric lysins (P361, P362, P371, and P372), which were the fusion of Salmonella phage lysins and novel antimicrobial peptide LeuA-P, were obtained using bioinformatics analysis and in silico design. The recombinant chimeric lysins were expressed in E. coli BL21(DE3) strain and showed highly specific inhibition against Salmonella. The minimal inhibitory concentrations (MICs) of P362...

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