Journal of Applied Microbiology

Thu, 01/23/2020 - 03:16
Aims This study was conducted to early detect the negative culture bacterial pathogens causing subclinical mastitis for the fast diagnosis of the disease and the reduction of some milk transmitted pathogenic bacteria to human consumers. Methods and Results A total of 171 positive California mastitis test (CMT) milk samples collected from asymptomatic dairy cows in Sharkia Governorate, Egypt were...
Sat, 01/18/2020 - 03:15
Aims To study association between number and positions of mutations with MICs of fluoroquinolone non‐susceptible Haemophilus influenzae. Methods and Results More than 40% of 48 H. influenzae isolated from nursing home residents were not susceptible to fluoroquinolone. Amino acid changes in the quinolone resistance determining regions, and correlation with MICs and inhibition zone diameters were...
Sat, 01/11/2020 - 03:15
Aim Following previous research on improving the cleaning of crates used to transport broiler chickens from the farm to the abattoir, a demonstration project was undertaken to investigate improvements in crate washing on a commercial scale. Methods and Results The soak tank of a conventional crate washing system was replaced with a high‐performance washer fitted with high‐volume, high‐pressure...
Sat, 01/04/2020 - 03:17
Aim To effectively make high‐quality silage in hot and humid area. Methods and Results The natural lactic acid bacteria (LAB) strains CZ149, XH358, XH753, and XH761 isolated from corn and Napier grass were screened by the potential of low pH growth and high lactic acid production, and their effect on silage quality, aerobic stability and aflatoxin B1 production of whole‐crop corn were also...
Sun, 12/29/2019 - 03:17
Aims Assessment the quality of fresh whitemouth croaker (Micropogonias furnieri) collected in different seasons the through quantification of histamine‐forming bacteria, determination of histamine content, and characterization of microorganism communities. Methods and Results The histamine content of fresh whitemouth croaker was detected by HPLC‐DAD with a concentration ranging from 258.52 to 604...
Tue, 12/24/2019 - 03:16
Aims This study evaluated the antimicrobial resistance of Salmonella enterica strains from layer poultry farms in central Ecuador isolated during 2017. This geographical area is responsible for around 60% of total domestic egg production, yet, as of 2019, no reports had been published on the phenotypic and genotypic antibiotic resistance patterns of Salmonella in the layer poultry farms of this...
Tue, 12/24/2019 - 03:16
Aim Identification and enumeration of foodborne pathogens in food stuffs are valuable concerns. In the present study, starch‐blood‐egg yolk‐polymyxin B‐trimethoprim‐ceftazidime (SBYPTC) agar was established to isolate and specify the number of B. cereus in food products. Methods and results The effectiveness of the developed medium in selecting for B. cereus from pure cultures and food matrixes...
Wed, 12/18/2019 - 03:27
Aim Infiltration experiments aimed to show the effectivity of coral sand to remove microorganisms in septic tank wastewater treatment system, in South Tarawa, Kiribati. Methods and Results Laboratory experiments evaluated effective microbial removal properties of a packed bed of coral beach sand conditioned with domestic effluent. Bacterial and viral indicators Escherichia coli J6‐2, Enterococci...
Sat, 12/07/2019 - 03:17
Aim The objective of this study was to investigate the biofilm inhibitory activity of Streptomyces‐derived actinomycin D against biofilm formation by Staphylococcus epidermidis. Methods and Results The microtiter plate method and microscopy were used to detect the biofilm formation of S. epidermidis. And an attempt was made to detect the effect of actinomycin D on important biofilm components,...
Fri, 12/06/2019 - 03:16
Consumers’ demand is increasing for safe foods without impairing the phytochemical and sensory quality. In turn, it has increased research interest in the exploration of innovative food processing technologies. Cold plasma technology is getting popularity now days owing to its high efficacy in decontamination of microbes in fruit and fruit based products. As a on‐thermal approach, plasma...
Wed, 12/04/2019 - 03:17
Aim The present investigation was aimed at isolating and identifying bacterial strains from cured vanilla beans. Additionally, the study focused on evaluating bacterial processes pertaining to the aromatic compounds production (ACP). Methods and Results Three bacteria were isolated from Vanilla planifolia beans, previously subjected to the curing process. According to morphological, biochemical...
Sun, 12/01/2019 - 03:16
Aim To develop a novel in vitro urethra model and use it to determine if insertion of an intermittent urinary catheter (IC) displaces pathogenic bacteria from the urethral meatus along the urethra. Methods Displacement of microbial growth after catheter insertion was assessed using a novel in vitro urethra model. The in vitro urethra model utilised chromogenic agar and was inoculated with...
Thu, 11/14/2019 - 03:16
Aims The identification and differentiation of antibiotic resistant bacteria by MALDI‐TOF‐MS profiling remains a challenge due to the difficulty in detecting unique protein biomarkers associated with this trait. To expand the detectable proteome in antibiotic‐resistant bacteria, we describe a method implementing offline LC protein separation/fractionation prior to MALDI‐ToF‐MS and top‐down MALDI‐...
Thu, 11/14/2019 - 03:16
Aims To prevent the spread of brucellosis, a simple and rapid vertical flow technology for the detection of antibodies targeting brucellosis was developed. Methods and Results In this study, Brucella spp. lipopolysaccharide (LPS) was purified and used to detect brucellosis antibodies. Sheep IgG was used as a negative control. Colloidal gold‐labeled recombinant staphylococcus aureus protein A was...
Wed, 11/13/2019 - 03:16
Aims Current culture‐based methods for detection and determination of Campylobacter levels on processed chickens takes at least two days. Here, we sought to develop a new complete, low‐cost and rapid (approximately 2·5 hours) detection system requiring minimal operator input. Methods and Results We observed a strong correlation between culture‐based cell counts and our ability to detect either...
Wed, 11/13/2019 - 03:16
Aims To investigate the prevalence and characteristics of Salmonella in pork on sale in Wuhan, Central China. Methods and Results A total of 4744 pork samples were collected from retail or wholesale markets in each season from 2016 to 2018. The samples showed an overall Salmonella prevalence of 19·54% (927/4744), among which the samples collected in 2017 (21·67%, 428/1975) possessed a...
Sat, 10/26/2019 - 03:21
Shiga toxin‐producing Escherichia coli (STEC) is a zoonotic pathotype associated with human gastrointestinal disease that may progress to severe complications. Ruminants, especially cattle, are the main reservoirs of STEC from which they can contaminate the environment and foods of animal or vegetable origin. Besides Shiga toxin, other virulence factors are involved in STEC virulence. O157:H7...
Sat, 10/26/2019 - 03:21
Aims To develop a pcsB‐based Loop‐mediated isothermal amplification (LAMP) method for detection of Streptococcus agalactiae (GBS) in milk, tilapia, and vaginal swabs. Methods and results The sensitivity of the LAMP method using real‐time turbidity monitoring was 1 pg of template within 1 h at 64°C, 100‐fold higher than conventional PCR. The sensitivity of visual detection dropped an order of...
Tue, 10/15/2019 - 03:22
Aims The object of this study was to identify potential health concerns of the Aeromons spp. isolated from marketed Pacific abalone (Haliotis discus hannai) with respect to their virulence and antimicrobial resistance patterns. Methods and Results We identified 29 strains of aeromonads consisting of 5 species; A. hydrophila (n=9), A. enteropelogenes (n=14), A. veronii (n=3), A. salmonicida (n=2)...
Tue, 10/15/2019 - 03:22
Aims This study was aimed at revealing the composition of microbiota in leaves, roots and rhizosphere soil of wheat plants that are resistant or susceptible to stripe rust, one of the most widely destructive leaf diseases in wheat production. Methods and Results A total of 36 wheat plants that showed resistant or susceptible reactions to stripe rust were sampled. Three compartments of each plant...
Sat, 10/12/2019 - 03:19
Aim To study Weissella cibaria and W. confusa strains, Lactic Acid Bacteria (LAB) members, naturally present in food products, but not yet included in Qualified Presumption of Safety (QPS) list of European Food Safety Authority (EFSA). Methods and Results We carried out a comparative genome analysis of 23 sequenced W. cibaria and seven W. confusa genomes, in parallel with a physiological and...
Sat, 10/12/2019 - 03:19
Aim To evaluate antimicrobial effect of paeoniflorin against carbapenem‐resistant Klebsiella pneumoniae (CRKP). Methods and Results Minimum inhibitory concentration (MIC) of paeoniflorin against CRKP was determined by agar dilution method. Changes in intracellular ATP concentration, intracellular pH (pHin), cell membrane potential and membrane integrity were investigated to assess the influence...
Mon, 09/30/2019 - 17:28
Lactic acid bacteria (LAB) are widely distributed in nature and, due to their beneficial effects on the host, are used as probiotics. This review describes the applications of LAB in animal production systems such as beekeeping, poultry, swine and bovine production, particularly as probiotics used to improve health, enhance growth and reproductive performance. Given the importance of honeybees in...
Sat, 09/28/2019 - 03:14
Aims To study the antimicrobial agents of the Bacillus velezensis strain HC6 and assess the application potential of Bacillus velezensis HC6 in maize. Methods and Results We applied a dual culture technique to test the antimicrobial activity of B. velezensis HC6 against bacteria and fungi of common contaminated crops. B. velezensis HC6 showed antagonistic action on pathogenic fungi, including...
Sat, 09/28/2019 - 03:14
Aims This study investigated the efficacy of Hydrogen Peroxide Vapour (HPV) at inactivating Hazard Group 3 bacteria that have been presented dried from their growth medium to present a realistic challenge. Methods and Results HPV technology (Bioquell) was used to decontaminate a class III microbiological safety cabinet containing biological indicators (BIs) made by drying standard working...
Mon, 09/23/2019 - 03:15
ABSTRACT Aim:  To identify a DNA sequence specific to a bacterium found in poultry litter that was indicative of faecal contamination by poultry sources. Methods and Results:  Faecally‐contaminated poultry litter and soils were used as source material for development of a quantitative polymerase chain reaction (qPCR) method targeting the 16S rRNA gene of a Brevibacterium sp. The identified...
Mon, 09/23/2019 - 03:15
Acinetobacter baumannii causes several nosocomial infections and poses major threat when it is multidrug resistant. Even pan drug‐resistant strains have been reported in some countries. The intensive care unit (ICU) mortality rate ranged from 45.6% to 60.9% and it is as high as 84.3% when ventilator‐associated pneumonia was caused by XDR (extensively drug resistant) A. baumannii. Acinetobacter...
Mon, 09/23/2019 - 03:15
Actinomycetes are a rich source for secondary metabolites with a diverse array of biological activities. Among the various genera of actinomycetes, the genus Saccharopolyspora has long been recognized as a potential source for antibiotics and other therapeutic leads that belong to diverse classes of natural products. Members of the genus Saccharopolyspora have been widely reported from several...
Mon, 09/23/2019 - 03:15
Aims Suilysin (SLY), a crucial virulence‐related factor, has multiple cytotoxicities that are regarded as playing a key role in several diseases induced by Streptococcus suis. The aim of this study was to identify an effective inhibitor of SLY and to evaluate the potential inhibitory effect of the inhibitor against S. suis virulence. Methods and Results Antibacterial activity experiments and...
Mon, 09/23/2019 - 03:15
Aim The increase in the number of fungal infections worldwide, coupled with the limitations of current antifungal chemotherapy, demand the development of safe and effective new antifungals. Here, we presented the synthesis of a novel acridone (M14) and its antifungal properties against Candida and dermatophytes species. Methods and Results A series of 17 acridones was designed, synthesized and...
Mon, 09/23/2019 - 03:15
Aims To assess the bacterial diversity in the French egg processing industry and to explore the adhesion and spoilage potential of selected bacteria. Methods and Results Sterile stainless steel chips were suspended for 2 months inside the pipelines of seven egg processing companies, before and after the pasteurizer, at warm and cold seasons. After exposure, the bacterial diversity was assessed by...
Mon, 09/23/2019 - 03:15
Aims To investigate the effects of ohmic heating (OH) and water bath heating (WB) on the membrane permeability, membrane structure, intracellular organization and leakage of intracellular substances of Escherichia coli O157:H7 at the same inactivation level and at a heating temperature of 72°C. Methods and Results Flow cytometry analysis indicated that membrane permeability of E. coli O157:H7 by...
Mon, 09/23/2019 - 03:15
The production of livestock and poultry faces major challenges to meet the global demand for meat and dairy products and eggs due to a steady increase in the world’s population and the ban of antibiotics in animal production. This ban has forced animal nutritionists to seek for natural alternatives to antibiotics. In this context, the yeast Saccharomyces cerevisiae has received considerable...
Mon, 09/23/2019 - 03:15
Aims Predicting bacterial resistance provides valuable information that can assist in clinical decisions. With recent advances in whole genome sequencing technology, the detection of antibiotic resistance (AR) proteins directly from genomic data is becoming feasible. AR genes/proteins can be identified using best‐hit methods that work by comparing candidate sequences with known AR genes in public...
Mon, 09/23/2019 - 03:15
Aims To determine the seasonal occurrence and diversity of norovirus (NoV) and human adenovirus (HAdV) in groundwater from sinkholes, and brackish water used for recreational activities in the karst aquifer of the Yucatan Peninsula, Mexico. Methods and results Hollow fibre ultrafiltration was used to concentrate viruses and standard plaque assay methods were used to enumerate somatic and F+...
Mon, 09/23/2019 - 03:15
Aims The aim of this study was to evaluate the microbiological quality of commercially prepared RTE sushi by enumerating aerobic mesophilic bacteria (AMB) and thermotolerant coliforms (TC) and detecting Escherichia coli and Salmonella spp. An isolate was identified as E. coli O157:H7 which was evaluated for its virulence and antimicrobial resistance profiling as well as its ability to form...
Wed, 09/11/2019 - 12:54
Abstract Aims The aim of this study was to develop a rapid detection and differentiation method for pathogenic Listeria species in stone fruits. Methods and Results We utilized activated charcoal enrichment media (ACM) to induce overexpression and hypersecretion of pathogenic Listeria virulence proteins which can subsequently be detected via immunoblot analysis. Plum and nectarine slices...
Wed, 09/11/2019 - 05:03
Abstract Aims To evaluate antibacterial properties of Methylsulfonylmethane (MSM) on Escherichia coli (MDRE21) and Salmonella enterica serovar Kinshasa (SK132). Methods and Results Bacterial proliferation analysis was measured spectrophotometrically during log phase growth with 0, 3, 5, 7, 10, 12 and 16% MSM. To assess the mechanism of inhibition, cultures were grown overnight with 0–16% MSM...
Wed, 08/28/2019 - 10:24
Abstract Aims Given the significance of Salmonella enterica to both human and animal health, and a recent global dissemination of Salmonella 4,[5],12:i:‐, changes in the prevalent serovars and antimicrobial resistance in clinical isolates of Salmonella from cattle and pigs were investigated in Japan. Methods and Results The serovars and antimicrobial susceptibilities of 1,605 Salmonella...
Wed, 08/28/2019 - 08:10
Abstract In ruminants, high fermentation capacity is necessary to develop more efficient ruminant production systems. Greater level of production depends on the ability of the microbial ecosystem to convert organic matter into precursors of milk and meat. This has led to increased interest by animal nutritionists, biochemists and microbiologists in evaluating different strategies to manipulate...
Sun, 08/25/2019 - 13:54
Abstract Aims This study evaluated the efficacy of essential oil from Origanum vulgare L. (oregano; OVEO) and Rosmarinus officinalis L. (rosemary; ROEO) to inactivate sessile cells of Salmonella enterica serovar Enteritidis 86 (SE86) in young and mature biofilms formed on stainless steel. Methods and Results Ultrastructural alterations and damage in different physiological functions caused by...
Tue, 08/20/2019 - 12:53
Abstract Aims An extensive source investigation was conducted on a dairy farm with neurolisteriosis and subclinical mastitis cases to identify infection source and potential transmission routes of Listeria monocytogenes. Methods and Results A total of 36 L. monocytogenes isolates were obtained from animal clinical cases (neurolisteriosis and udder infection) and the farm environment (silage,...
Mon, 08/19/2019 - 13:53
Abstract The production of livestock and poultry faces major challenges to meet the global demand for meat and dairy products and eggs due to a steady increase in the world's population and the ban of antibiotics in animal production. This ban has forced animal nutritionists to seek for natural alternatives to antibiotics. In this context, the yeast Saccharomyces cerevisiae has received...
Tue, 08/13/2019 - 14:54
Abstract Aims To investigate the effects of ohmic heating (OH) and water bath heating (WB) on the membrane permeability, membrane structure, intracellular organization, and leakage of intracellular substances of E. coli O157:H7 at the same inactivation level and heating temperature of 72 °C. Methods and Results Flow cytometry (FCM) analysis indicated that membrane permeability of E. coli O157:...
Fri, 08/09/2019 - 15:58
Abstract Aims First, two inactivation models were compared for different phenotypic profiles of Escherichia coli O26 using ultraviolet‐C light (UV‐C) and thermal treatment (T), by means of Central Composite Rotatable Design of Experiment (CCRD). Second, we aimed to evaluate the subsequent survival and persistence of cells in simulated gastric fluid (SGF). Methods and Results Two strains of E....
Wed, 08/07/2019 - 05:22
Abstract Aims We developed a strategy for localized delivery of the LFCA (lactoferricinlactoferrampin), which is actively synthesized in situ by Lactococcus lactis (pAMJ399‐LFCA/LLMG1363), and explored the possibility of using pAMJ399‐LFCA/LLMG1363 as an alternative additive diet to antibiotics. Methods and Results The antimicrobial activities of the LFCA derived from pAMJ399‐LFCA/LLMG1363...
Wed, 08/07/2019 - 05:22
Abstract Aims Manila clam (Ruditapes philippinarum) is one of the most popular seafood in Korea, owing to their unique taste and nutritional value. This study aimed to disclose the antibiotic and heavy metal resistance characteristics of Aeromonas spp. isolated from marketed Manila clam in Korea. Methods and results A total of 36 Aeromonas spp. strains were isolated and subjected to two tests...
Wed, 08/07/2019 - 05:22
Abstract Aim Study is focused on the influence of cadmium addition to growth media on production yield, their size and molecular mass of exopolysaccharides (EPS) synthesized by three rhizosphere bacteria strains. Inhibition of bacterial growth by increasing concentrations of Cd2+ was also analysed. Methods and Results The highest impact of Cd2+ was noticed on the growth of Arthrobacter sp. and...
Tue, 08/06/2019 - 13:29
Abstract Aims This work aimed to evaluate the antimicrobial activity of pure (ZnO) and doped (ZnMgO) zinc oxide nanoparticles on bacterial pathogens and Saccharomyces cerevisiae to confirm their applicability as an alternative to antibiotics and to estimate their biocompatibility. Methods and Results Microbial growth inhibition on agar plates, microbial viability and adaptation tests in broth...
Thu, 08/01/2019 - 12:18
Abstract Aim The molecular typing and the susceptibility of Staphylococcus aureus strains of swine origin to antibiotics, oregano (Origanum vulgare L.) essential oil (EO) and Chilean blackberry maqui (Aristotelia chilensis (Molina) Stuntz) extract were determined. Methods and Results Twenty S. aureus strains of swine origin were subjected to molecular typing, of which six strains were selected...

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