Journal of Food Protection

Sat, 06/19/2021 - 04:02
Ubiquity of Listeria monocytogenes in the environment impacts the food industry and presents concerns for frozen food facilities. This study determined the prevalence and numbers of Listeria species and L. monocytogenes on raw produce arriving at frozen food facilities. Raw produce was collected using multi-level blinding protocols to ensure anonymity of participants and avoid traceback. Five raw...
Wed, 06/16/2021 - 04:03
Significant resources including both human and financial capital have been dedicated to developing and delivering food handler training programs to meet government and organizational mandates. Even with the plethora of food safety-oriented training programs, there is scant empirical evidence documenting their effectiveness suggesting the need to rethink the design and delivery of food safety...
Wed, 06/16/2021 - 04:03
Experiments were performed to assess the survival of Salmonella on whole Brazil nuts and pumpkin seeds stored at 8ºC, 23ºC, and 37ºC. Brazil nut kernels and pumpkin seeds were inoculated with bacterial inoculum containing 10.4 Log 10 CFU/ml of Salmonella Enteritidis Phage Type 30 ( S E PT30) and then aseptically dried at room temperature for 24 hrs. After the drying step, levels of Salmonella...
Wed, 06/16/2021 - 04:03
Fresh Milk Bar (FMB), an emerging dairy retail franchise, is used to instantly produce and sell pasteurized milk and other dairy products in China. However, the quality and safety of pasteurized milk in FMB have received little attention. The objective of this study is to investigate the prevalence, antimicrobial resistance, and virulence genes of Escherichia coli (E. coli) , Staphylococcus...
Sat, 06/12/2021 - 04:02
L. monocytogenes causes relatively few outbreaks linked to whole fresh produce but triggers recalls each year in the US. There are limited data on the influence of wet vs. dry methods on pathogen growth on whole produce. A cocktail of five L. monocytogenes strains that included clinical, food, or environmental isolates associated with foodborne outbreaks and recalls was used. Cultures were...
Sat, 06/12/2021 - 04:02
Bottled tea beverages (BTB) are popular for the benefits to human health and convenience. Because Chlorpyrifos (CP) is commonly used as a biomarker for exposure as well as a pesticide in the field, it is important to determine the dynamics of CP dissipation in BTB in order to better perform risk assessments. This study focused on the dynamic behavior of CP for 22 days by fortifying bottled green...
Sat, 06/12/2021 - 04:02
The human noroviruses are major causes of nonbacterial gastroenteritis, and are transmitted by both food and water, as well as via a person-to-person route. Asymptomatic norovirus infection of food handlers may play a role in transmission. The outbreak of noroviruses infections has been recognized in PyeongChang Winter Olympics, starting with security staffs from February 3, 2018. The Ministry of...
Sat, 06/12/2021 - 04:02
The application of plant extracts (PEs) could be a promising option to satisfy consumers’ demand for natural additives to inhibit growth of variable pathogenic bacteria. Thus, the aim of this study was to develop a standardized microdilution method to examine the antimicrobial effects of ten hydrophilic plant extracts against two strains of C. perfringens facing various food-relevant influencing...
Sat, 06/05/2021 - 04:02
Toxoplasma gondii , hepatitis E virus (HEV) and Salmonella are zoonotic foodborne pathogens that may be transmitted to humans through the consumption of raw or undercooked pork.  The aim of this study was to determine the seroprevalence of anti- Toxoplasma gondii , anti-HEV and anti- Salmonella antibodies from healthy pigs at slaughter in Switzerland. In the period of August to September 2020...
Sat, 06/05/2021 - 04:02
This study aimed to investigate the inactivation of foodborne pathogens and the quality characteristics of fresh chicken breasts after Ultraviolet-C light-emitting diode (UVC-LED) treatment. Fresh chicken breasts were separately inoculated with Salmonella Typhimurium, Escherichia coli O157:H7, and Listeria monocytogenes at an initia population of 6.01, 5.80, and 6.22 log 10 CFU/cm 2 ,...
Sat, 06/05/2021 - 04:02
Poultry remains one of the top food commodities responsible for foodborne illness in the U.S., despite poultry industry efforts since the inception of HACCP to reduce the burden of foodborne illness implicating poultry products. The appropriate use of antimicrobial compounds during processing of raw poultry can help minimize this risk. Currently, peroxyacetic acid (PAA) is the most popular...
Sat, 06/05/2021 - 04:02
The International Food Safety Authorities Network (INFOSAN) is a global network of national food safety authorities from 190 countries, managed jointly by the Food and Agriculture Organization of the United Nations (FAO) and the World Health Organization (WHO), which aims to facilitate the rapid exchange of information during food safety incidents. A three-phase study of INFOSAN was launched in...
Sat, 06/05/2021 - 04:02
In recent years, science and technology have developed to a considerable level. However, food contamination by food-borne pathogens is still widespread in many countries around the world, and food safety is a major global public health issue. Therefore, novel preservatives that can guarantee safer food are high in demand. Contrary to artificial food preservatives, tea polyphenols (TP) are getting...
Sat, 06/05/2021 - 04:02
Study was carried out to evaluate the incidence of mycobiota and contamination of AFB 1  in sesame seeds from rainfed and irrigated zones of the Punjab, Pakistan. For this purpose, 100 sesame seed samples were collected directly from the fields of major sesame-producing areas of the rainfed and irrigated zone. Samples were subjected to the Agar Plate Method for the isolation of mycobiota and thin...
Sat, 06/05/2021 - 04:02
The first step in quantitative microbial risk assessment (QMRA) is to determine distribution of pathogen contamination among servings of the food at some point in the farm-to-table chain. In the present study, distribution of Salmonella contamination among servings of chicken liver for use in QMRA was determined at meal preparation. A combination of five methods: 1) whole sample enrichment; 2)...
Sat, 05/29/2021 - 04:02
Environmental and health advocates are increasingly promoting food donations to reduce landfilled food waste and feed hungry people. Share tables are locations where students can put unwanted school food or beverage items, allowing their uneaten food items to be “shared” with other students and providing food donation opportunities for the 4.9 billion lunches served annually in the U.S. National...
Sat, 05/29/2021 - 04:02
Salmonella is a common cause of foodborne illness in the U.S. and often is attributed to chicken products. Previous studies have associated Salmonella contamination with meat processing facility characteristics such as the number of establishment employees (i.e., HACCP size). An evaluation of risk factors for Salmonella contamination in U.S. poultry has not been performed since implementation of...
Sat, 05/29/2021 - 04:02
The U.S. is one of the largest catfish producers in the world. Louisiana is the leading producer of wild-caught catfish. Historically, the FDA inspected all seafood products; however, in 2008, congress moved the Siluriformes Order fish inspection to the USDA/FSIS. Full enforcement of the rule began on September 1, 2017. This study assessed the impact of USDA Siluriformes fish regulation on small...
Sat, 05/29/2021 - 04:02
Listeria monocytogenes is a pathogenic bacterium associated with RTE meat products sold at the retail level. The objective of this research was to determine the prevalence of Listeria monocytogenes in RTE meat products sold at retail in Costa Rica and to study the factors associated with the levels of contamination; analyzed factors include hygienic practices within stores (cutting techniques,...
Sat, 05/29/2021 - 04:02
Plasma-activated water (PAW) is a new sanitizer, which has received great attention for application in food industries. This research aimed to evaluate the effect of PAW on the inactivation of Shewanella putrefaciens and quality attributes of Yellow River carp ( Cyprinus carpio ) fillets. The carp fillet samples were immersed in sterile deionized water (SDW) or PAW120 (plasma discharge on the...
Sat, 05/29/2021 - 04:02
Listeriosis, a foodborne illness caused by Listeria monocytogenes , has relatively low incidence, but a substantial mortality rate, particularly in immunocompromised populations. Because of the known risk of L. monocytogenes and other pathogens in produce, immunocompromised individuals are often placed on neutropenic diets that exclude fresh produce. Therefore, this study aimed to evaluate...
Fri, 05/21/2021 - 04:02
This study was conducted to compare the efficacy of two sanitizing agents (chlorine and PAA) in reducing (both spoilage and pathogenic) microorganisms and in reducing disinfection by-products ( DBPs) in the washing stage of three types of minimally processed vegetables: Iceberg lettuce, carrots and baby leaves. These fresh-cut products are consumed uncooked and, hence, a proper sanitation is...
Fri, 05/21/2021 - 04:02
Little is known about the prevalence of antimicrobial resistant (AMR) bacteria in veal meat in the United States.  We estimated the prevalence of bacterial contamination and AMR in various veal meats collected during the 2018 U.S. National Antimicrobial Resistance Monitoring System (NARMS) survey of retail outlets in nine states and compared to the frequency of AMR bacteria from other cattle...
Fri, 05/21/2021 - 04:02
Traditional microbiological testing methods are slow, and many molecular-based techniques rely on culture-based enrichment to overcome low limits of detection. Recent advancements in sequencing technologies may make it possible to utilize machine learning (ML) to identify patterns in microbiome data to potentially predict the presence or absence of pathogens. In this study, 299 poultry rinsate...
Fri, 05/14/2021 - 04:02
Histamine is a biogenic amine and a food safety hazard, and it is the only biogenic amine regulated by statute or HACCP Guidance. This paper reviews the regulations for histamine levels in fish in countries around the world, including maximum limits or levels and sampling procedures in different fish preparations. The maximum histamine levels, sampling plans, and fish products are listed. The...
Fri, 05/14/2021 - 04:02
Food labeling is one approach to encourage safe, healthy, and sustainable dietary practices. Consumer buy and pay preferences to specially labeled food products (e.g., USDA Organic, Raised Without Antibiotics, and Locally Raised) may promote the adoption of associated production practices by food producers. Thus, it is important to understand how consumer buy and pay preferences for specially...
Fri, 05/14/2021 - 04:02
New Zealand (NZ) has a high rate of reported campylobacteriosis cases. Cross-contamination in home kitchens during poultry handling is considered to be the main factor in campylobacteriosis transmission. The main aim of this study was to measure NZ consumers' food safety awareness and self-reported food safety practices associated with handling raw poultry. This study will contribute to the...
Fri, 05/07/2021 - 04:02
In the present study, we aimed to investigate the presence, seasonal distribution and biomolecular characterization of Vibrio parahaemolyticus and Vibrio vulnificus in samples of bivalve molluscs ( Mytilus galloprovincialis, Crassostrea gigas, Ruditapes decussatus, Venus verrucosa and Solen vagina ) and gastropod molluscs (Murex brandaris) harvested and marketed in Sardinia (Italy) between 2017...
Fri, 05/07/2021 - 04:02
Edible insects are a novel food in most countries; their popularity is growing because of their high protein/low fat content, ease of cultivation, and small environmental impact. To our knowledge, this is the first report that addresses both microbiological and chemical hazards in edible insects. Samples were collected from retail stores or purchase through e-commerce. A total of 51 samples of...
Fri, 05/07/2021 - 04:02
This study isolated Bacillus cereus from soft soybean curds and developed a dynamic model to describe the kinetic behavior of B. cereus isolates during transfer and storage. B. cereus were first isolated from soft soybean curds. A mixture of B. cereus isolates was then inoculated in soft soybean curd and the bacterial cell counts were enumerated during storage at 10-30°C. The B. cereus cell...
Fri, 05/07/2021 - 04:02
Essential foods of a daily meal have been reported to comprise of numerous kinds of biogenic amines (BAs) at different levels. BAs have a variety of toxicological impacts on human health, and they have been connected to multiple outbreaks of foodborne disease. They also are known to cause cancer based on their ability to react with nitrite salts resulting in the production of a carcinogenic...
Tue, 04/27/2021 - 04:02
Kefir is an acidic-alcoholic fermented milk that can provide probiotic benefits such as intestinal microecological balance regulation, antibacterial activity, and anti-inflammatory activity. In this study, Lactobacillus fermentum grx08 isolated from longevous people was used to further improve the health properties of kefir. L. fermentum grx08 and kefir grains obtained from Xinjiang, China were...
Tue, 04/27/2021 - 04:02
Science-based guidance employed at eight small and very small state and federally inspected ready-to-eat (RTE) meat and poultry processors across Michigan was assessed. Data was collected to determine the current level of sanitary control methods used for reducing Listeria in the processing environment and compared interactions with the facility microbial results. A checklist was created to...
Wed, 04/21/2021 - 04:02
Plastics provide tremendous societal benefits and are an indispensable part of our lives. However, fragmented plastics or those intentionally manufactured in small sizes (microplastics or nanoplastics) are of concern because they can infiltrate soils and enter the human food chain through trophic transfer. The pathophysiological impacts of micro/nanoplastics in humans are not characterized but...
Wed, 04/21/2021 - 04:02
Recent revisions to USDA FSIS compliance and safe harbor guidelines for ready-to-eat meat and poultry products addressed process humidity requirements. Given the lack of prior data for impingement-cooked products, this project aimed to evaluate the impact of process humidity on Salmonella lethality at the product core and surface, and compliance of the results with USDA FSIS lethality performance...
Wed, 04/21/2021 - 04:02
Black yeasts are a functional group that has caused spoilage in cold-filled and hot-filled beverages as well as other water activity (a w )-controlled food products. We established quantitative thermoresistance parameters for the inactivation of 12 Aureobasidium and Exophiala isolates through isothermal experiments and a challenge study. Culture age (2-day vs. 28-day) variably affected the...
Wed, 04/21/2021 - 04:02
In this study, we compared the efficiency of culture-based methods with or without membrane filtration, real-time PCR and digital droplet PCR (ddPCR) for the detection of Campylobacter in fresh produce. Alfalfa sprouts, clover sprouts, coleslaw, and lettuce salad spiked with Campylobacter jejuni ( C. jejuni ) were enriched in Bolton broth for 48 h and enrichment cultures were either directly...
Thu, 04/15/2021 - 04:02
Growth on solid media as sessile cells is believed to increase the desiccation tolerance of Salmonella enterica . However, the reasons behind increased resistance have not been well explored. In addition, the same effect has not been examined for other foodborne pathogens such as pathogenic Escherichia coli or Listeria monocytogenes . The purpose of this research was two-fold: first, to determine...
Thu, 04/15/2021 - 04:02
The aim of the present study was to evaluate the contamination levels of some classes of persistent organic pollutants (POPs) in free-range hen eggs and to estimate the related human dietary exposure in a Site of National Interest (SNI), characterized by a serious state of environmental pollution (Bussi sul Tirino area, central Italy). For these purposes, 17 samples of free-range hen eggs...
Thu, 04/15/2021 - 04:02
Pseudomonas fluorescens is a specific spoilage microorganism of refrigerated marine fish, which possesses strong adaptability to low temperature. Cold shock proteins (CSPs) play an important role in bacterial cold adaptation. In this study, the CSP genes were obtained from the genome of P. fluorescens PF08 by search the conserved domain of CSPs through HMMER software, and their physicochemical...
Sat, 04/10/2021 - 04:02
Leafy greens contaminated with Shiga toxin-producing Escherichia coli (STEC) have continued to cause foodborne illness outbreaks in recent years and present a threat to public health. An important component of foodborne illness outbreak investigations is determining the source of the outbreak vehicle through traceback investigations. The Food and Drug Administration is home to traceback...
Sat, 04/10/2021 - 04:02
The growth and hemolysin production of two V.   alginolyticus  strains (HY9901 and ATCC17749T) at 30 °C in briny tilapia, shrimp, scallop, oyster, pork, chicken, freshwater fish and egg fried rice were investigated. Bacterial counts were enumerated by plate counting. Hemolysin production was evaluated by blood agar and hemolytic titer tests. The two V.   alginolyticus  strains displayed similar...
Fri, 04/02/2021 - 04:02
Hydrogen peroxide (H 2 O 2 ) has long been studied as an aqueous sanitizer to enhance microbial safety of fresh produce. Recently, we demonstrated that cold plasma-activated H 2 O 2 aerosols, hereafter referred to as ionized hydrogen peroxide (iHP), reduced populations of Salmonella, Listeria, and E. coli by up to 5.5 logs on surfaces of various produce items. However, the amount and fate of H 2...
Fri, 04/02/2021 - 04:02
Contamination with potentially pathogenic microorganisms may occur at all stages of the food chain. We conducted a representative cross-sectional survey of 1008 consumers aged 16 years and older in Germany via an online panel; the aim was to assess the perception of consumers regarding the influence of aspects of meat production on the safety and quality of meat. Over 70 % of the participants...
Fri, 04/02/2021 - 04:02
Here, a Bacillus strain, UTK D1-0055, is described that was isolated from a laboratory environment. It was of interest as it demonstrated antilisterial activity. The genome was sequenced, which identified the strain as Bacillus altitudinis, and a high-quality complete annotated genome was produced. Additionally, the taxonomy of this and related species was evaluated, including B. aerophilus, B....
Fri, 04/02/2021 - 04:02
Bacillus cereus, which causes foodborne disease, is detected using selective media. However, competing flora is the most common factor preventing the correct enumeration of B. cereus on selective agars. In this study, we aimed to improve the selectivity of mannitol-yolk-polymyxin B agar (MYPA) and its modified version containing trimethoprim (mMYPA) developed in our previous study by...
Fri, 04/02/2021 - 04:02
Recently, ready-to-eat vegetable salads have gained popularity worldwide. However, the microbial safety of ready-to-eat salads is a health concern, primarily due to Salmonella enteritidis contamination during the growing, harvesting, processing, and handling of produce. This study aimed to develop a bacteriophage-based strategy to control S. enteritidis growth in mixed-ingredient salads. A lytic...
Fri, 04/02/2021 - 04:02
Dried parsley is regularly contaminated with foodborne pathogens, especially Salmonella (S.) spp. Application of contaminated ingredients in ready-to-eat dishes without further thermal treatment represents a considerable health risk. This study examines the suitability of pulsed light as a novel decontamination method of Salmonella spp. in dried parsley, the impact on selected quality parameters...
Sat, 03/27/2021 - 04:02
This study investigated the effects of in-feed encapsulated cinnamaldehyde (CIN) and citral (CIT) alone or in combination (CIN+CIT) on antimicrobial resistance (AMR) phenotypes and genotypes of E. coli isolated from feces of 6-, 16-, 23- and 27-days old broiler chickens. The five dietary treatments including the basal diet (control; NC), the basal diet supplemented with 55 ppm bacitracin (BAC),...
Sat, 03/27/2021 - 04:02
The increase in fat-related diseases and chemical hazards produced during the frying process poses a major threat to human health. Coating has been used as a practical method to reduce the oil content and chemical hazard of fried foods. Methyl cellulose (MC) and soy protein isolate (SPI) were used as coating materials to pretreat Chinese fried dough cake (CFDC) before frying. The results showed...

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