Journal of Food Safety

Tue, 10/20/2020 - 22:12
The research showed that ruminant animal is the primary factor contributing to the prevalence of the STEC‐infecting phages on the organic farm in this study. Among environmental factors, high rain precipitation is likely to facilitate phage distribution in the surrounding environment of the farm. Most importantly, the presence of these phages has a negative correlation with their STEC hosts....
Fri, 10/16/2020 - 22:13
Fast detection for heavy metal in vegetables is one of the most important steps to ensure the food safety. A novel method to identify lead pollution levels of lettuce based on hyperspectral image technology was proposed in this study. Firstly, hyperspectral images of lettuce samples cultivated under four lead stress levels (0 mg/L, 50 mg/L, 100 mg/L and 200 mg/L) were collected using...
Tue, 10/13/2020 - 22:11
P. hydropiper ethanolic extract: Chlorogenic acid, ferulic acid, rutin, myricetin andquercetin. P. hydropiper essential oil: dodecanal, caryophyllene, caryophyllene oxide, decanal, α‐caryophyllene, citronellol, heptadecanal, linalool and phytol. Ethanolic extract and essential oil of P. hydropiper exhibited antimicrobial activity against three bacteria Staphylococcus aureus 6538P, Escherichia...
Thu, 10/08/2020 - 22:12
Co‐microencapsulation of lactobacilli cell free and propolis extract inhibited bacterial growth considerably. Abstract Cell free extracts (CFE) obtained from Lactobacillus plantarum FI 8595 and Lactobacillus reuteri ATCC 55730 alone or in combination with propolis ethanolic or water extracts (1%) were microencapsulated with maltodextrin (25%) before the subsequent spray drying process. They were...
Wed, 09/23/2020 - 22:12
Botrytis rot is a serious disease of fruit and vegetables around the globe. We tested and found that frequencies above 5 kHz and mechanical vibrations and acoustic frequencies from cold‐storage devices increase the growth rate of botrytis rot, suggesting that acoustic output from storage units could promote the mold during transport, storage, or within grocery stores. Results open new...
Thu, 09/17/2020 - 22:12
Antimicrobial peptides (AMPs) are powerful microbial inhibitors with high potential in food preservation, despite this, their use as preservatives is very limited and one of the main reasons is the concern about their stability to food processing conditions. This manuscript offers a different view of the application of AMPs in food preservation, addressing the relationship between food processing...
Tue, 09/15/2020 - 22:13
PEF treatment of sesame seeds provides significant reduction on aflatoxin producing fungi, Aspergillus paraciticus, as well as decomposition of aflatoxins with reduced mutagenity with preservation of physical properties and reduced acid number and peroxide value. Abstract Seed processing technologies are essential for seed safety and functionality through protection of physicochemical quality,...
Thu, 09/10/2020 - 22:12
In this study, the rainbow trout was stored for 18 days. Changes in the resistance levels in head, meat and eyes, changes of color and odor in the head and meat, changes of liquid levels in the skin and meat, were measured with Arduino. Study showed that the resistance value on the meat decreased as the storage period increased; and that the color, odor and liquid level measurement sensors could...
Wed, 09/02/2020 - 22:13
Coronaviruses, enveloped non‐segmented positive‐sense RNA viruses, distinguished in the mid‐60s can infect humans and a variety of birds and mammals. The purpose of this study was to review these coronaviruses, especially SARS (because of its very similar gene sequence to the 2019‐nCoV or SARS‐CoV‐2), from the perspective of observing the food plant health principles including observing the...
Thu, 08/27/2020 - 22:13
• Salmonella enterica subsp. enterica serotypes Enteritidis and Typhimurium were identified as the predominant serotypes (prevalence 3.35%) in the poultry (live chicken, 2.5%) and poultry products (carcass meat 4.83% and table eggs 2.15%).• Salmonella isolates produced virulence determinants associated with invasion (invA gene, 100%), toxin production (spvR gene, 77.27% and spvC gene, 22.72%) and...
Tue, 08/18/2020 - 22:12
A microfluidic device was applied to enumerate Escherichia coli O157:H7 cells in lettuce and beef samples. E. coli O157:H7 inoculated in lettuce and beef were collected with high efficiency by using filtration or two‐step centrifugation. This method could quantify target microbial cells within 1 hr. Abstract Quality assurance is one of the fundamental ways of preventing infections from foodborne...
Tue, 08/18/2020 - 22:12
Escherichia coli O157:H7 entered into the Viable but Non Culturable (VBNC) state under salinity stress of 10 and 30% during production of salted silver carp. VBNC E. coli O157:H7 induced by NaCl stress during salted fish production continued to express its pathogenic genes of stx1, stx2, eae and hly. Abstract Many bacteria including Escherichia coli can enter into the viable but non‐culturable (...
Tue, 08/18/2020 - 22:12
Vibrio parahaemolyticus is one of the most hazardous pathogens causing seafood‐borne diseases in the southern Fujian coast, China. From June to October 2016, a total of 250 samples were collected from retail markets in the Xiamen, Quanzhou, and Zhangzhou regions. Seventy‐seven V. parahaemolyticus isolates were identified using polymerase chain reaction (PCR). Then, molecular typing was performed...
Tue, 08/18/2020 - 22:12
Aspergillus, Penicillium, Fusarium, and Alternaria species which produce toxic metabolites, create a big problem in terms of the production of reliable human food and animal feeds. This study was conducted to determine the mycotoxin contents of foodstuff collected uniformly in each year of 2017, 2018, and 2019 in Şebinkarahisar, Turkey by enzyme‐linked immunosorbent assay. The highest total...
Tue, 08/18/2020 - 22:12
In this study, the effectiveness of ACP in inactivating Aspergillus flavus inoculated to military rations snack using response surface methodology (RSM) was investigated. Additionally, the effect of this treatment on the total count and yeast–mold count, as well as some quality properties of military rations snack, was examined. RSM was applied to study the voltage effects (5–15 kV), the distance...
Thu, 08/13/2020 - 22:12
A new selective broth, SSEL, was developed for simultaneous enrichment of S. enterica, S. aureus, E. coli O157: H7, and L. monocytogenes, optimizing by single factor experiments and a L9 (34) orthogonal test. Developed SSEL broth not only enriched the target pathogens to 5 log10 CFU/ml after 18 hr incubation at 37°C with 10–100 CFU/mL of inoculation concentration, but also could successfully...
Tue, 08/11/2020 - 22:12
Isochoric freezing, different from isobaric (conventional) freezing, has a superior capability to reduce foodborne pathogens (a) photo and (b) schematic of a prototypical isochoric chamber. Abstract Isochoric freezing, different from isobaric (conventional) freezing, allows for storage below freezing temperatures without significant damage from ice formation. While several types of tissues have...
Mon, 08/10/2020 - 22:12
We have developed a visual on‐site detection of African swine fever by the denaturation bubble‐mediated strand exchange amplification with colorimetric detection strategy. Combined with rapid extraction, the entire detection process takes only 50 min and only requires a portable metal bath. African swine fever virus was successfully detected in eight different tissues and seven types of pig feed...
Fri, 08/07/2020 - 22:12
Freshly harvested Bambara groundnut seed obtained from rural framers in the Ehlanzeni District Municipality of the Mpumalanga province, South Africa does not have detectable levels of mycotoxins. Freshly harvested Bambara groundnut seed were naturally contaminated with mycotoxin producing isolates from the genera Aspergillus and Fusarium. Abstract Freshly harvested Bambara groundnut (BGN) is...
Thu, 08/06/2020 - 22:11
Antifungal effects of smoldering plants are studied in vitro and pistachio nuts. The smoke of the cinnamon plant had an in vitro fungicidal effect in the PDA medium. No aflatoxin was observed in pistachios treated by cinnamon bark smoke. A reliable method is introduced for pistachio farmers to provide safe products. Abstract Aflatoxin (AF) contamination of the Iranian exporting pistachio has...
Wed, 08/05/2020 - 22:11
The image of a probe thermometer as a nudge tool significantly improved compliance with and control of the critical control points. Citrus scent with a short inscription significantly improved the hygiene behavior of the food handlers at all critical stages of food preparation. Pictures and short inscriptions turned out to be the most effective nudge tools to improve the food handlers' hygiene...
Tue, 08/04/2020 - 22:11
This study investigated antimicrobial efficacy of trans‐cinnamaldehyde (TC) and chitosan (CH) along with high pressure processing (HPP) against Cronobacter sakazakii in infant formula. All treatments exhibited varying degrees of C. sakazakii reductions up to undetectable levels. Remarkable deformation and damage in C. sakazakii cells were observed by transmission electron microscopy after the...
Tue, 08/04/2020 - 22:11
Two monoclonal antibodies (mAbs) specific to Salmonella were produced from a mouse immunized with a combination of heat‐killed whole‐cell and formalin‐fixed Salmonella enterica serovar Enteritidis (S. Enteritidis) as the antigens. The mAb SE‐13D bound specifically only to S. Enteritidis, while mAb Sal‐06G bound to various members of the Salmonella serogroups B, C, D, E, and I. Neither mAb...
Tue, 07/28/2020 - 22:11
The developed miniaturized test (s) rapidly detects Escherichia coli/coliforms colorimetrically utilizing specific enzyme substrates and selective agents incorporated in media. High sensitivity and selectivity for target bacteria. A total of 139 milk samples were tested for presence of E.coli/coliforms using developed test (s). Abstract Current investigation was aimed to develop colorimetric...
Sat, 07/25/2020 - 22:09
Lateral‐flow immunochromatographic assay (LFIA) was performed on pastry samples incubated for different time‐periods and the degree of coliform contamination evaluated. Pastries contaminated with ~5.0 log10 (cfu/g) coliforms showed LFIA positivity by 3 hr culture, whereas pastries contaminated with 2.0 log10 (cfu/g) coliforms showed LFIA positivity by 9 hr culture. The degree of coliform...
Wed, 07/15/2020 - 22:12
NaHB4, Te powder, CdCl2 and mercaptopropionic acid under a dark place were synthesized to get CdTe quantum dots (QDs) precursors, the precursors were furtherly reacted with zinc acetate and sulfur acetamide to obtain CdTe/ZnS QDs. The CdTe/ZnS QDs were covalently coupled with streptavidin by EDC•HCL and succinimide in the next step. The CdTe/ZnS QDs and Antibody were linked by avidin on the CdTe/...
Wed, 07/08/2020 - 22:11
Efficacy of N‐Halamine on Foodborne Pathogens Abstract A major concern of the cattle industry is cross‐contamination of meat with pathogens. Cattle are exposed to fecal material, mud, and other contaminants which harbor pathogens that can be shed onto meat and meat processing equipment. Due to increased chances of meat contamination during processing, new antimicrobial formulations for carcass...
Sat, 06/27/2020 - 22:10
The antimicrobial calcium‐alginate‐based films containing the self‐microemulsifying (SME) thyme essential oil (TEO) formulations were fabricated successfully. A Ca‐alginate film containing nano‐emulsified TEO as well as a neat Ca‐alginate film were considered as the controls. The SME films released the TEO completely within 155 min and inhibited the growth of S. aureus and E. coli in in vitro...
Fri, 06/19/2020 - 22:10
Nyjer and flax seeds are important oilseeds that are used for both human and animal consumption. Aspergilluscarbonariusproduced ochratoxin A (OTA) up to 271 μg/kg on ground nyjer seeds incubated with 0.98 awat 20°C, while A. carbonarius produced the highest OTA (146 μg/kg) on ground flax seeds incubated with 0.94 awat 20°C. Overall, ground nyjer seed is better than flax seed to support growth and...
Tue, 06/09/2020 - 22:11
Campylobacter is the most common cause of bacterial gastroenteritis worldwide and the most frequently reported foodborne pathogen in the European Union (EU). The predominant source of infection is poultry. The reduction of Campylobacter spp. in the poultry meat chain in the EU can only be achieved with an integrated meat safety assurance approach. Abstract Campylobacter is the most common cause...
Fri, 06/05/2020 - 03:28
Microbial inspections were conducted at a hospital kitchen in both Vietnam and JapanThe mean general viable bacteria found on cookware and equipment in the Vietnamese hospital kitchen was significantly higher than in the Japanese kitchen.Coliform and E. coli were detected in cooked foods in the Vietnamese, but not the Japanese, hospital kitchen. Abstract This study aimed to assess food safety in...
Thu, 05/28/2020 - 03:29
Antimicrobial effect of oregano and thyme essential oils (1%) in minced pork was assessed after storage at 3°C/7 days in a vacuum. The inhibition of S. Typhimurium depended mainly on the fat content (logarithmic dependence) and to a lesser degree on the pH, whereas pH was not found to be a significant predictor in the case of L. monocytogenes. The results show that only lean minced meat (up to 5...
Thu, 05/28/2020 - 03:29
Royal jelly (RJ) and propolis were able to inhibit the growth of Aspergillus parasiticus with minimum inhibitory concentrations (MICs) of 3,200 and 100 μg/ml, respectively. Combination of RJ and propolis resulted in synergistic interaction with MICs of 200 and 25 μg/ml, respectively, and a fractional inhibitory concentration index of 0.312. RJ and propolis were able to down‐regulate the...
Mon, 05/25/2020 - 03:26
A novel selective enrichment broth (SLB) combined with a loop‐mediated isothermal amplification (LAMP) kit was developed for inspection of Salmonella in ready‐to‐eat fruits and vegetables. A novel selective enrichment broth was developed for the inspection of Salmonella. The recovery of injured Salmonella in SLB was superior to Selenite Cystine Broth. The growth of Salmonella and inhibitory non‐...
Fri, 05/08/2020 - 03:27
The main compounds of LC‐EO were citral and nerol. In the present study, gram‐negative bacteria had greater sensitivity to LC‐EO than gram‐positive bacteria. LC‐EO could lead to damage to the cell wall and membrane, resulting in the leakage of cell contents, such as electrolytes and nucleic acids, and eventual cell death. Abstract Litsea cubeba essential oil (LC‐EO) has been reported as...
Thu, 05/07/2020 - 03:26
PDT is an emerging new antimicrobial technology, which uses a combination of photosensitizers and light. This study, for the first time, demonstrated that ERY (Red No. 3)‐mediated PDT can be an alternative antimicrobial treatment strategy to the conventional treatments used in tomato juice. Abstract The objectives of this study were to evaluate the efficacy of erythrosine B (ERY, Red No. 3)‐...
Wed, 05/06/2020 - 03:28
The present study investigated the effects of combined ultrasound (37 kHz, 380 W for 5 min) and peroxyacetic acid (PAA; 50–200 ppm) treatment on the reduction of Salmonella Typhimurium and Campylobacter jejuni on chicken skin. Ultrasound was not sufficient to inactivate S. Typhimurium (0.48 log CFU/g reduction) or C. jejuni (0.25 log CFU/g reduction), whereas PAA significantly (p < .05)...
Sun, 04/26/2020 - 03:24
S. enteritidis and P. aeruginosa had an inhibitory effect on L. plantarum. L. plantarum had no effects on S. enteritidis and P. aeruginosa. Lotka–Volterra model with six interaction coefficients could quantitatively describe the interaction among Salmonella enteritidis, Lactobacillus plantarum, and Pseudomonas aeruginosa. Abstract This paper was to investigate the interactions among Salmonella...
Fri, 04/24/2020 - 03:25
Cronobacter sakazakii (C. sakazakii) is an opportunistic, neonatal, and food borne pathogen primarily associated with the contamination of powdered infant formula (PIF). The pathogen is reported to overcome the food safety barriers such as increased acidity, heat treatment, and so on. This study evaluates the thermal tolerance of C. sakazakii strains independently at 52, 55, and 58°C in...
Fri, 04/24/2020 - 03:25
Innovation in colorimetric sensors to detect early signs of food spoilage is a growing interest in food safety and quality control. Currently available chemical dyes for sensor applications have concern because of their possible migration into food packaging and food in contact. In this study, a colorimetric indicator was developed using natural butterfly pea (Clitoria ternatea) anthocyanin with...
Thu, 04/23/2020 - 03:24
Graphical Abstract Sodium hypochlorite (NaClO) combined with ultrasonic cleaning (USC) significantly reduced the TVC of crab materials. Antibacterial treatments combined with natural chemicals can be utilized commercially to maintain the quality and safety of marinated raw crab paste during storage at −20°C. Abstract The effects of antibacterial treatments and several natural chemicals on the...
Thu, 04/23/2020 - 03:24
Prolonged exposure of foodborne pathogens (Salmonella Enteritidis [SE], Escherichia coli O157:H7 [EC], and Listeria monocytogenes [LM]) to acid, salt, and cold stress alters their antibiotic resistance. However, the effect of acid, salt, and cold stress on bacterial antibiotic resistance depend on both the bacterial species and the specific antibiotic. Abstract Bacteria with antibiotic‐resistant...
Thu, 04/23/2020 - 03:24
The study was conducted to characterize Staphylococcus aureus strains from swabs and drips of dressed chicken carcasses sold at outlets in six townships in the informal market in Gauteng province, South Africa, using molecular and phenotypic methods. Seven genes (6 toxins and 1 antimicrobial resistance) comprising staphylococcal enterotoxin A (SEA), B (SEB), C (SEC), D (SED), exfoliative toxin A...
Thu, 04/09/2020 - 03:21
High oleic peanuts were inoculated with Enterococcus faecium and roasted in a convection oven at 190°C. There was a 6 log CFU/g reduction at 480 s. D‐values for high oleic peanut butter at 110, 120, and 125°C were 438.9, 165.1, and 80.6 s, respectively. The z‐value was calculated to be 20.8°C. High oleic peanut butter was inoculated with E. faecium and agitated in a heated mixer for 21.5 min. E....
Tue, 04/07/2020 - 03:32
This study aimed at evaluating the physicochemical and microbiological quality, besides the occurrence of aflatoxins M1 and B1, in samples (229) of the whole, semi‐skimmed and skimmed milk processed by the ultra‐high‐temperature system, in the three Brazilian southern states (Rio Grande do Sul, Santa Catarina and Paraná). After incubation at 37°C for 7 days, it was found, a sample that had...
Tue, 04/07/2020 - 03:32
Fresh‐cut apples were dip‐coated in antimicrobial/antibrowning solutions. The developed solutions had the dual effectiveness of both controlling browning and reducing pathogenic bacterial populations, which maintained the fresh‐like appearance and texture of apple slices for 35 days and reduced populations of Salmonella to undetectable levels (<1 log) without growth throughout the storage at 4...
Mon, 04/06/2020 - 03:17
This study was aimed to evaluate the efficiency of the organic chlorine and acetic acid solutions on the inactivation of adhered cells of Escherichia coli, Cronobacter sakazakii and Klebsiella pneumoniae to lettuce. Besides, the growth and inactivation of K. pneumoniae adhered to lettuce was modeled. According to the findings, the use of chlorine solution (170 mg/ml of total residual chlorine)...
Mon, 04/06/2020 - 03:17
Viable but nonculturable (VBNC) cells were able to recover at 25°C, but their recovery extent was affected by the types of prestressed treatment and strain. Cold‐stressed, and tetracycline‐resistant Vibrio cells were more rapidly converted to VBNC state than nonstressed cells. V. cholerae has a higher risk than V. parahaemolyticus and V. vulnificus in frozen seafood. Abstract We compared the fate...
Thu, 04/02/2020 - 03:17
The real‐time recombinase polymerase amplification (RPA) could complete the reaction at 35°C in 20 min, which is much faster and cheaper than real‐time polymerase chain reaction. Salmonella enterica serovars, Salmonella enteritidis and Salmonella typhimurium, were detected simultaneously by multiplex real‐time RPA method for the first time. The LOD of S. enteritidis and S. typhimurium without...
Fri, 03/27/2020 - 03:30
Gaseous chlorine dioxide (ClO2) is a promising sanitizer for frozen products because of its efficacy under nonthermal and waterless conditions. Our pilot‐scale treatment of blueberries demonstrated that a significant reduction of 1.5 log CFU/g can be achieved against coliforms after ClO2 exposure. Our findings demonstrate a cost‐effective procedure that could be adapted to commercial processing....

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