Journal of Food Safety

Tue, 10/08/2019 - 03:21
The aim of this study was to examine the resistome characteristics of Salmonella spp. Contamination found in raw retail beef, pork, and chicken samples collected in Hanoi, Vietnam. Approximately 28% (25/90) of samples tested carried Salmonella bacteria. A total of 13/25 (52%) of these Salmonella bacteria were resistant to at least one antibiotic and 9/25 (36%) of the isolates were found to be...
Tue, 10/08/2019 - 03:21
The current study investigates the effect of Lactobacillus reuteri and Lactobacillus plantarum combined with water extract of garlic on microbial growth, chemical changes, and sensory attributes in ground beef samples at refrigeration condition (+4°C) up to 12 days of storage. in vitro study revealed that garlic extract combined with L. reuteri or L. plantarum caused 2.13 and 2.57 log reduction...
Sat, 10/05/2019 - 03:21
This study investigated the combined effects of different doses of UV‐C light at 260 nm (0–1,800 mWs/cm2) and ethanol (0–70%) on the reduction of Escherichia coli and Bacillus cereus in experimentally contaminated Gwamegi (semidried Pacific saury). After combined treatment of 4,800 mWs/cm2 of UV‐C and 70% ethanol, E. coli and B. cereus were reduced by 3.03 and 2.73 log CFU/g, respectively....
Wed, 09/11/2019 - 09:53
Abstract Detection of Salmonella on commercially processed broiler carcasses is essential to reduce the incidences of human salmonellosis. This study was designed to compare the standard procedure for Salmonella detection with two commercial screening methods. During seven separate visits to two processing plants, eight carcasses were obtained (n = 112). Each carcass was rinsed and the rinsate...
Thu, 09/05/2019 - 05:31
Abstract Sweeping frequency ultrasound washing is a recent technology of applying varying ultrasound frequencies in the decontamination of fresh produce. Ultrasound inactivation of Escherichia coli and Listeria innocua inoculated on fresh‐cut Chinese cabbage was investigated at sweeping frequencies of 28 ± 2 to 68 ± 2 kHz for washing time of 5–40 min. The survival ratio of E. coli and L. innocua...
Mon, 09/02/2019 - 05:45
Abstract The mechanisms of inactivation against Gram‐negative bacteria (GNB) including Pseudomonas aeruginosa, Escherichia coli, and Vibrio parahaemolyticus, and Gram‐positive bacteria (GPB) involving Listeria monocytogenes and Staphylococcus aureus, by dielectric barrier discharge cold atmospheric plasma were elucidated. Both GNB and GPB were exposed to treatment with cold plasma produced from...
Fri, 08/30/2019 - 08:33
Abstract The antibacterial activity and the mechanism of Tetrapleura tetraptera root extract against Escherichia coli and Staphylococcus aureus were investigated. The mechanism of action of T. tetraptera on tested bacterial species was predominantly characterized by the study of transmission electron microscopy, which revealed severe damage on the primary target which affected the cell integrity...
Fri, 08/30/2019 - 08:30
Abstract This research was done on the assumption that the risk for hepatocellular carcinoma (HCC) development that attributed to the consumption of highly contaminated food (HCF) with aflatoxin, may have a major contribution to the total of expected risk. If this hypothesis is true, it is easy to apply an effective and low‐cost intervention approach by excluding HCF and then subjecting it to...
Thu, 08/29/2019 - 15:52
Abstract The present research was carried out to determine the presence of Listeria monocytogenes in the equipment, staff, and products in the chicken shredding facilities operating in Samsun, and to serotype and genotype the isolates by PCR and PFGE analysis, respectively. Of the total of 192 samples tested, 25 were found to be L. monocytogenes positive from which 51 isolates were acquired. In...
Thu, 08/29/2019 - 15:34
Abstract The efficacy of the natural plant‐derived compound, eugenol (EG), as an antimicrobial wash treatment to reduce Campylobacter jejuni in postharvest poultry was investigated. The antimicrobial efficacy of EG was studied as a suspension, emulsion, or nanoemulsion treatment (two trials each). In each trial, chicken skin samples were inoculated with C. jejuni (∼7.2 Log CFU/sample), washed...
Wed, 08/28/2019 - 09:24
Abstract Spring water is an important direct drinking water resource. Enterococcus faecalis is a significant opportunistic pathogen from environment that causes human neonatal sepsis and urinary tract infections. Our study investigated the prevalence, virulence genes, antimicrobial resistance, and molecular characterization of E. faecalis from spring water in Guangdong, China. A total of 285...
Tue, 08/27/2019 - 11:13
Abstract This study was aimed to investigate the effects of lactic acid stress with acid adaptation on the survival and expression of virulence‐related genes in Escherichia coli O157:H7 (strains CICC21530, 89, 95, 112, 109, and J42). E. coli were subjected to acid adaption (pH 5.0) for 1–4 hr and then exposed to acid stress (pH 3.5) for 30–120 min. Results showed that exposure to pH 3.5...
Tue, 08/27/2019 - 10:11
Abstract The study investigated the effects of (E)‐2‐hexenal on morphology and function of Penicillium cyclopium spores, and these conidia were treated with (E)‐2‐hexenal at various concentrations. (E)‐2‐hexenal at 8× minimum fungicidal concentration (8× MFC, 2560 μl/L) severely damaged conidia membranes through scanning electron microscopy, which resulted in the changes in membrane permeability...
Tue, 08/27/2019 - 10:11
Abstract The primary aim of this study was to obtain a cost‐effective nanofiber (NCNF) on the surface of aluminum foil. The main aim of the study was to investigate the microbial quality parameters of fish flesh wrapped by aluminum foil coated with NCNF. NCNF having 128.98 nm average diameter and −2.26 mV zeta potential was successfully obtained. NCNF was utilized to delay the rapid increase in...
Wed, 08/21/2019 - 15:39
Abstract Production of aflatoxin by Aspergillus flavus in peanuts is both a health hazard and major problem. This study investigated the ability of a whey protein concentrate (WPC)‐based edible coating containing Williopsis saturnus var. saturnus to prevent growth of A. flavus and aflatoxin production in peanuts. WPC with/without W. saturnus (7 log colony‐forming unit [CFU]/g) or W. saturnus...
Wed, 08/21/2019 - 08:22
Abstract This investigation we evaluated the feasibility of using the insulated isothermal polymerase chain reaction (iiPCR) reagent in a POCKIT system to detect Staphylococcus aureus in foods by comparing its results with those obtained using quantitative PCR (qPCR). The iiPCR/POCKIT system could specifically detect S. aureus and Staphylococcus spp. and differentiate them from 10 other...
Mon, 08/19/2019 - 08:08
Abstract This study focused on significant hazard analysis, the determination and establishment of prerequisite programs, and the role of critical control point (CCP) based on ISO 22000:2018, providing guidelines and experiences related to the incorporation of significant hazard analysis and the use of the CCP decision tree approach to determine and update the possible CCPs of seven primary food...
Mon, 08/19/2019 - 08:08
Abstract Listeria monocytogenes is a foodborne pathogen widely distributed in nature. The aims of this study were to evaluate the occurrence of Listeria spp. in three dairy plants located in Southern Brazil, and to characterize the L. monocytogenes isolates according to serotypes, virulence genes, and PFGE. A total of 212 samples were collected, and 15 were positive for Listeria spp., of which...
Fri, 08/16/2019 - 09:35
Abstract In this study, we examined the effect of ethyl pyruvate on the efficacy of the inactivation of Listeria monocytogenes on the surface of sausage samples by using quantitative PCR (Q‐PCR). Propidium monoazide (PMA) was used for direct quantification of viable and dead cells. The samples inoculated with L. monocytogenes (~3 to 4 log CFU/mL) and uninoculated samples were treated with 0, 42...
Thu, 08/01/2019 - 15:53
Abstract Essential oils (EOs) have long been recognized for their medicinal properties. Application of EOs in food preservation has gained recent interest as natural alternatives to conventional chemicals. Even though the properties of EOs are extensively documented, their effect on several food‐related microorganisms is yet to be explored. In this study, six EOs were extracted by...
Thu, 07/25/2019 - 14:09
Abstract The objective of the present study was to conduct a survey to evaluate the postharvest handling of peanuts at farm level and to screen and identify the strains of Aspergillus and Penicillium species from peanut kernels in central Myanmar. A total of 640 samples from the stores of farmers, collectors, and wholesalers were collected and seeded for growth, isolation, and characterization...
Thu, 07/25/2019 - 08:48
Abstract The main safety issue of cereal grains is mycotoxin contamination. There is no published information about mycotoxin contamination of organic wheat in the United States. This study quantified the contents of deoxynivalenol (DON) and ochratoxin A (OTA), two frequently occurring mycotoxins, in organic wheat samples collected from six counties in North Carolina by enzyme‐linked...
Mon, 07/22/2019 - 08:24
Abstract The aim of this study was to assess the microbiological quality and safety of typical dry fermented sausages produced in Apulia region, Southern Italy, classified as “Traditional Agri‐Food Products” (T.A.P.). A total of 106 samples were collected and analyzed. Two samples of Soppressata di maiale nero dei Monti Dauni (1.89%) were positive to Listeria monocytogenes. Nevertheless, the...
Tue, 07/16/2019 - 08:57
Abstract In this research, effects of chitosan coating of brown trout (Salmo trutta fario) fillets containing inulin were determined. Chitosan enriched by vegetable oils (0% Chitosan‐control, 2% acetic acid +Chitosan, 2% sage oil+Chitosan, and 2% mint oil+Chitosan) on shelf life was investigated. In the experiment, fish fillets from the control and the experimental groups were subjected to...
Sun, 07/14/2019 - 13:23
Abstract The present study tested the antibacterial activity, expressed as minimum bactericidal concentration (MBC), of zinc oxide nanoparticles (ZnO‐NPs), copper oxide nanoparticles (CuO‐NPs) and their combination with or without rosemary, clove or cinnamon extract against Escherichia coli O157:H7 and Listeria monocytogenes. The NPs were characterized by scanning electron microscopy. The sizes...
Tue, 07/09/2019 - 14:30
Abstract In the present work, physicochemical, sensory characteristics and viability of Bifidobacterium bifidum and Lactobacillus casei of probiotic yogurt using dill (Anethum graveolens) essential oil (DEO) (50 and 100 ppm) were investigated at 4°C for 21 days. Alterations in amount of fat and protein of yogurt treatments during storage time were not significant. The viability of B. bifidum and...
Tue, 07/09/2019 - 05:40
Abstract Today, antibiotic resistance is one of the most crucial problems so that researchers have begun to search for alternatives to antibiotics. Among these alternatives, phage therapy and phytotherapy became prominent. The present study aims to put forward a different approach by combining these two methods. For this purpose, Staphylococcus aureus phages were isolated from commercial...
Wed, 07/03/2019 - 13:08
Abstract A real‐time loop‐mediated isothermal amplification (Rti‐LAMP) DNA assay was developed for rapid and sensitive detection of viable Escherichia coli O157:H7 in milk by combining with ethidium monoazide bromide (EMA) and bentonite coated activated carbon (BCAC) treatment without enrichment. The assay involved inoculating 25 mL portions of milk with various numbers of E. coli O157:H7. Then...
Wed, 07/03/2019 - 13:04
Abstract In this study, we investigated the antibacterial activity of hexahydro‐β‐acids/methyl‐β‐cyclodextrin inclusion complex (HBA/M‐β‐CD) and researched its antibacterial mechanism against Staphylococcus aureus and Enterobacter sakazakii. The result showed that bacteria had different sensitivities to HBA/M‐β‐CD with diameters of inhibition zones ranging from 7.8 ± 0.5 to 14.8 ± 0.7 mm and...
Tue, 07/02/2019 - 12:33
Abstract The study aimed at investigating the occurrence and molecular characterization of zoonotic Anisakis larvae in imported, retail‐marketed smoked herring (Clupea harengus) and frozen mackerel (Scomber scombrus) in Egypt. A total of 154 fishes (84 ready‐to‐eat smoked herring and 70 frozen mackerel) were collected. The prevalence of Anisakis larvae Type I in the whole specimen of smoked...
Tue, 07/02/2019 - 12:23
Abstract In this study, it was aimed to determinate the prevalence of extended spectrum β‐lactamases (ESBL) producing Enterobacteriaceae and genes responsible for resistance formation in cheeses that were sold in local markets in Samsun. A total of 150 cheese samples were collected from the local markets in Samsun center and were used as material. One hundred and forty‐eight ESBL positive...
Thu, 06/27/2019 - 05:08
Abstract This study was focused on determining survival and growth characteristics of Listeria monocytogenes and Staphylococcus aureus on conventional (16.7 mg of sodium/g) and low‐sodium (10.7 mg of sodium/g) bacon. The two types of bacon were inoculated with the either L. monocytogenes or S. aureus stored at 4, 12, or 25°C for up to 7 days. Populations of L. monocytogenes and S. aureus did not...
Tue, 06/25/2019 - 08:43
Abstract Sensory analysis is performed in USFDA testing laboratories to determine the decomposition status of seafood samples. Current alternatives are not always directly comparable to sensory testing. In this study, a novel method was explored using LC‐HRMS with untargeted analysis and a statistical model driven by sensory results via a script written in the R programming language. This allows...
Tue, 06/25/2019 - 08:35
Abstract Salmonella‐contaminated raw food is an important source of human food poisoning. Traditional methods for detecting Salmonella in meat involve many time‐consuming and laborious steps, preventing timely and effective control of the pathogen. In this study, a multiplex polymerase chain reaction (PCR) assay that targets serovar‐specific genes such as A7P63_0910 (Salmonella Indiana), sdfI (...
Mon, 06/24/2019 - 08:33
Abstract The purpose of this study was to assess the risk of zearalenone (ZEA) in corn meal produced and commercialized in State of Parana, Brazil. The extraction method carried out with a Romer Mycosep 226 column was found to be efficient due to addition of both diatomaceous earth and centrifuging during the cleaning step. From the total of 84 analyzed samples, 78.6% (n = 66) were found to be...
Mon, 06/24/2019 - 07:45
Abstract Rolling circle amplification (RCA) has been widely used to enhance detection signals as its long single‐stranded RCA products can provide multiple binding sites for signal probes for sensitive detections. In the current study, we employ atomic force microscopy (AFM) to monitor the RCA products during the course of the RCA process over time. Subsequently, the results of the RCA obtained...
Wed, 06/19/2019 - 05:29
Abstract This study aimed to evaluate the ability of the Lactobacillus rhamnosus strain LC‐4 to bind aflatoxin M1 (AFM1) in phosphate‐buffered saline (PBS) and yogurt. Bacterial cells were subjected to heat, acid, and alkali treatments. The AFM1‐binding rate of acid‐treated bacteria was of 78.63 ± 0.52%. The binding of L. rhamnosus LC‐4 to AFM1 was partially reversible and the binding of AFM1...
Mon, 06/17/2019 - 13:44
Abstract The detoxification ability of Lactobacillus acidophilus and prebiotics was evaluated by a Plackett‐Burman Design to examine the reduction of concentration and bioaccessibility of aflatoxin B1 (AFB1) and M1 (AFM1) in artificially contaminated whole cow's milk. Six variables were evaluated: AFB1 (3.25–6.0 μg L−1) or AFM1 (1.0–2.0 μg L−1) concentration; incubation time (0–6 h); and inulin,...
Tue, 06/11/2019 - 04:54
Abstract This study aimed to functionalize the stainless steel surface with silver and to evaluate its antimicrobial capacity. For this purpose, stainless steel coupons coated or not with silver films were exposed to the presence of Escherichia coli and Staphylococcus aureus. The results of bacterial adhesion on the silver functionalized coupons showed lower counts of adhered S. aureus cells...
Tue, 06/11/2019 - 04:54
Abstract Concerns over the safety of nitrite and its role in the formation of nitrosamines have aroused considerable interest in developing alternative antimicrobial agents to replace nitrite. Plant‐derived antimicrobial agents could serve against food spoilage and pathogens. For the first time in this study, three semiempirical kinetic models, that is, exponential, homographic, and n‐order...
Sun, 05/26/2019 - 11:56
Abstract The bacteriological quality and safety of ready‐to‐eat (RTE) foods in Barbados were assessed in two separate studies conducted between 2014 and 2016. In Study 1, 206 food samples were processed for Salmonella spp. by conventional bacteriological methods. Polymerase chain reaction (PCR) and serotyping were used for confirmation. In Study 2, 120 food samples were processed for the total...
Thu, 05/23/2019 - 12:34
Abstract Listeria monocytogenes (L. monocytogenes) is a major concern in human food safety. Here, the development of a reverse transcription‐loop‐mediated isothermal amplification (RT‐LAMP) method, targeting the hly A gene of L. monocytogenes, to rapidly detect this important pathogen in food is described. The RT‐LAMP conditions were optimized and the following parameters established: 60 min,...
Thu, 05/23/2019 - 11:24
Abstract The abattoir sector plays a crucial role in controlling foodborne hazards, so insight in the incentives that motivate abattoirs to implement control measures is of great relevance to food safety risk managers and policy makers. Therefore, a cross‐sectional telephone survey was conducted to assess the attitudes and opinions of abattoirs in Belgium and the Netherlands toward preventive...
Wed, 05/22/2019 - 13:00
Abstract Listeria monocytogenes is a major foodborne pathogen that may contaminate liquid egg yolk (LEY). A background microbiota, purified and identified as Enterococcus faecium with a 99.0% probability, was found in pasteurized unsalted LEY. This study was conducted to investigate the thermal resistance of L. monocytogenes and the background microbiota in unsalted and 10% salted LEY at...
Tue, 05/21/2019 - 10:18
Abstract Escherichia coli O157:H7 (E coli O157:H7) is a highly virulent bacterial strain that is present in food, and as few as 10 cells can cause severe diseases. Rapid and sensitive bacterial detection is urgently required. In this study, we report a flow cytometry (FCM)‐based method for detecting E coli O157:H7 at the single‐cell level. Single E coli O157:H7 cells were obtained by a flow...
Fri, 05/17/2019 - 15:35
Abstract Pesticide residues in produce are not commonly monitored in developing countries. This study aims to determine whether washing and peeling has an effect on removing pesticide residues from guava fruits. Seventy‐five guava samples were purchased from Thai markets, and analyzed for 67 pesticides. Eighteen pesticides were detected in the guava samples. The incidence of pesticide detection...
Fri, 05/17/2019 - 15:30
The goal of this study was to evaluate factors causing low recovery of Campylobacter during regulatory sampling following acidified sodium chlorite (ASC) treatment of broiler carcasses and subsequent carry‐over into neutralizing buffered peptone water (nBPW) rinses. Solution alkalinity was shown to positively correlate with the presence of un‐reduced chlorite anion in the recovery broth,...
Fri, 05/17/2019 - 15:28
Abstract Adulteration of butter can occur when milk fat is replaced with cheaper oils due to the economic advantage of such substitution. With potential health risks on consumers, the issue raises alarming public health concerns. The adulteration of the Egyptian buffalo butter (EBB) with vegetable oils (VOs) was detected by conventional and advanced techniques. EBB was mixed with VOs at different...
Fri, 05/10/2019 - 05:05
Abstract Large yellow croaker (Larimichthys crocea) has rich protein content and high economic value, but has a short shelf life. Lipopeptides extracted from marine Bacillus megaterium were applied to the preservation of large yellow croaker and effectively increased the shelf life of large yellow croaker. The results showed that the total viable count (the total number of colonies), total...
Fri, 05/10/2019 - 05:04
Abstract Biofilms of Lactobacillus plantarum and Listeria innocua were grown under single‐ and mixed‐culture conditions in a bioreactor with either half‐strength brain heart infusion (BHI) medium or BHI medium with sodium dichloroisocyanurate (150 ppm) for 24 hr. A quantitative approach using plate counts and fluorescence in situ hybridization (FISH) was applied, representing the culturable and...

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