Journal of Food Safety

Wed, 01/20/2021 - 22:08
A total of ciprofloxacin‐resistant Escherichia coli isolates were obtained from 41.7% of bulk tank milk samples in Turkey. Furthermore, all isolates were characterized by PFGE, phenotypic and genotypic antimicrobial resistance profile and virulence gene content. Majority of isolates displayed different banding pattern and resistance to non‐quinolone antibiotics was commonly observed. Abstract The...
Wed, 01/20/2021 - 22:08
Schematic representation of test system for transfer of L. monocytogenes associated with soil particles to spinach leaves in the presence of antimicrobial agents. Abstract Sporadic contamination of produce with Listeria monocytogenes (Lm) is a recurrent problem. Ways for contamination to occur include transfer of the pathogen to produce from processing surfaces or soil particles to which it has...
Sat, 12/19/2020 - 22:08
Impact of COVID‐19 on food safety and security of the food supply chain. Viral icons represent the aggregate accumulation of viral particles in discreet links in the supply chain. The red arrows indicate parts of the food supply chain experience food loss and waste, which ultimately impact food and economic security. Abstract The COVID‐19 pandemic has ushered in a new era of food safety. To date...
Thu, 12/03/2020 - 22:09
The application of bacteriophage P100 to reduce incidence of Listeria sp. in ready‐to‐eat (RTE) food production environments was investigated. Data indicate the application of Listeria specific phage P100 in RTE food production environments can reduce incidence and be considered an additional intervention strategy for controlling this pathogen on NFCS. Abstract Listeria monocytogenes is a...
Thu, 12/03/2020 - 22:09
This study evaluated the effect of lactic acid stress on E. coli O26 biofilm formation at different temperatures. The strain 126Z with strong capacity and strain G13Z2 with medium capacity were selected for biofilm formation experiment in acidic TSB (pH 4.0 and 5.0, adjusted with lactic acid) at 15°C and 25°C, respectively. The effect of temperature on biofilm formation of different capacity...
Tue, 11/24/2020 - 22:10
The incidence of foodborne diseases has continuingly increased over the years and resulted in public health problem globally. Enterohemorrhagic Escherichia coli O157:H7 (E. coli O157:H7) is a human pathogen that causes diarrhea and hemorrhagic colitis. E. coli O157:H7 can be found in various foods. It is important to detect this foodborne pathogen to provide safe food supply. A lot of methods,...
Tue, 11/24/2020 - 22:10
A strip test for the detection of all serotypes of Vibrio cholerae was developed. Detection limit of the bacterium in seafood samples was 107 CFU/ml. The sensitivity could be improved to 1 CFU/ml after pre‐incubation in APW for 24 h. Due to its simplicity and rapidity, the sample could be directly tested at point of care. Abstract A nanogold‐based immunochromatographic strip test (VCG strip test...
Mon, 11/23/2020 - 22:10
This study evaluated the effects of sialic acid (SA) combined with Lactobacillus on the adhesion of S. aureus by three ways: competition, exclusion and displacement. It was found that 260 μg/ml SA combined with L. plantarum had the higher inhibition effect in the competition assays. As shown in the figure, compared with the control group (Figure A), after adding 260 μg/ml SA and L. plantarum Z‐4...
Fri, 11/20/2020 - 22:09
The scattering experiment is shown in Figure 1. The aptamer of Listeria monocytogenes is fixed on the bare electrode surface and its complementary DNA sequence (DNA1) labeled with bipyridyl ruthenium is self‐assembled to the electrode surface through base complementary pairing, which keeps the strongest electrochemical luminescence signal. Subsequently, the addition of the ferrocene‐labeled DNA...
Wed, 11/18/2020 - 22:09
Pathogenic bacterial contamination is a serious problem for the food industry and in public health. Rapid, accurate and affordable testing for pathogenic bacterial strains is desirable. In this study, a competition visual antigen macroarray (CVAM) for rapid detection of Escherichia coli O157:H7 (E. coli O157:H7) has been developed. This array was able to utilize an HRP‐labeled anti‐E. coli O157:...
Mon, 11/09/2020 - 22:13
A novel, label‐free, bidirectional lateral flow immunoassay strip that detected E. coli O157:H7 in food was developed, and it has good specificity, sensitivity, stability, and practicality. The low‐cost and low‐difficulty development of the strip rapid detection technology, which requires neither nano‐labeling materials nor heavily relies on antibodies, may be easier to realize commercialization...
Fri, 11/06/2020 - 22:12
Ohmic heating has long been used to inactivate pathogens in food products. Several research investigations on the use of ohmic heating technology in the inactivation of microbial hazards in food products are discussed in this review. These studies are discussed under the following sub‐headings: (a) inactivation of microbiological hazards, (b) in combination treatments with other sanitizing...
Wed, 10/28/2020 - 22:11
Self‐reported knowledge and practices of hospital food handlers’ reached satisfactory levels, however foodservices' hygienic‐sanitary conditions and microbiological analysis of food handling surfaces were inadequate. The results showed a need for motivating hospital food handlers to practice the safe handling knowledge that they have demonstrated in theory. Abstract The aim of the study was to...
Wed, 10/28/2020 - 22:11
Rapidity, sensitivity, and selectivity of the methods have been compared. Regulations of detection methods have been covered. Limitations and further studies have been suggested. Abstract The rapid, sensitive, and selective detection of foodborne pathogens is important to ensure food safety. Culture medium‐based methods for bacteria detection have long been used since Robert Koch's first finding...
Tue, 10/20/2020 - 22:12
The research showed that ruminant animal is the primary factor contributing to the prevalence of the STEC‐infecting phages on the organic farm in this study. Among environmental factors, high rain precipitation is likely to facilitate phage distribution in the surrounding environment of the farm. Most importantly, the presence of these phages has a negative correlation with their STEC hosts....
Fri, 10/16/2020 - 22:13
Fast detection for heavy metal in vegetables is one of the most important steps to ensure the food safety. A novel method to identify lead pollution levels of lettuce based on hyperspectral image technology was proposed in this study. Firstly, hyperspectral images of lettuce samples cultivated under four lead stress levels (0 mg/L, 50 mg/L, 100 mg/L and 200 mg/L) were collected using...
Tue, 10/13/2020 - 22:11
P. hydropiper ethanolic extract: Chlorogenic acid, ferulic acid, rutin, myricetin andquercetin. P. hydropiper essential oil: dodecanal, caryophyllene, caryophyllene oxide, decanal, α‐caryophyllene, citronellol, heptadecanal, linalool and phytol. Ethanolic extract and essential oil of P. hydropiper exhibited antimicrobial activity against three bacteria Staphylococcus aureus 6538P, Escherichia...
Thu, 10/08/2020 - 22:12
Co‐microencapsulation of lactobacilli cell free and propolis extract inhibited bacterial growth considerably. Abstract Cell free extracts (CFE) obtained from Lactobacillus plantarum FI 8595 and Lactobacillus reuteri ATCC 55730 alone or in combination with propolis ethanolic or water extracts (1%) were microencapsulated with maltodextrin (25%) before the subsequent spray drying process. They were...
Wed, 09/23/2020 - 22:12
Botrytis rot is a serious disease of fruit and vegetables around the globe. We tested and found that frequencies above 5 kHz and mechanical vibrations and acoustic frequencies from cold‐storage devices increase the growth rate of botrytis rot, suggesting that acoustic output from storage units could promote the mold during transport, storage, or within grocery stores. Results open new...
Thu, 09/17/2020 - 22:12
Antimicrobial peptides (AMPs) are powerful microbial inhibitors with high potential in food preservation, despite this, their use as preservatives is very limited and one of the main reasons is the concern about their stability to food processing conditions. This manuscript offers a different view of the application of AMPs in food preservation, addressing the relationship between food processing...
Tue, 09/15/2020 - 22:13
PEF treatment of sesame seeds provides significant reduction on aflatoxin producing fungi, Aspergillus paraciticus, as well as decomposition of aflatoxins with reduced mutagenity with preservation of physical properties and reduced acid number and peroxide value. Abstract Seed processing technologies are essential for seed safety and functionality through protection of physicochemical quality,...
Thu, 09/10/2020 - 22:12
In this study, the rainbow trout was stored for 18 days. Changes in the resistance levels in head, meat and eyes, changes of color and odor in the head and meat, changes of liquid levels in the skin and meat, were measured with Arduino. Study showed that the resistance value on the meat decreased as the storage period increased; and that the color, odor and liquid level measurement sensors could...
Wed, 09/02/2020 - 22:13
Coronaviruses, enveloped non‐segmented positive‐sense RNA viruses, distinguished in the mid‐60s can infect humans and a variety of birds and mammals. The purpose of this study was to review these coronaviruses, especially SARS (because of its very similar gene sequence to the 2019‐nCoV or SARS‐CoV‐2), from the perspective of observing the food plant health principles including observing the...
Thu, 08/27/2020 - 22:13
• Salmonella enterica subsp. enterica serotypes Enteritidis and Typhimurium were identified as the predominant serotypes (prevalence 3.35%) in the poultry (live chicken, 2.5%) and poultry products (carcass meat 4.83% and table eggs 2.15%).• Salmonella isolates produced virulence determinants associated with invasion (invA gene, 100%), toxin production (spvR gene, 77.27% and spvC gene, 22.72%) and...
Tue, 08/18/2020 - 22:12
A microfluidic device was applied to enumerate Escherichia coli O157:H7 cells in lettuce and beef samples. E. coli O157:H7 inoculated in lettuce and beef were collected with high efficiency by using filtration or two‐step centrifugation. This method could quantify target microbial cells within 1 hr. Abstract Quality assurance is one of the fundamental ways of preventing infections from foodborne...
Tue, 08/18/2020 - 22:12
Escherichia coli O157:H7 entered into the Viable but Non Culturable (VBNC) state under salinity stress of 10 and 30% during production of salted silver carp. VBNC E. coli O157:H7 induced by NaCl stress during salted fish production continued to express its pathogenic genes of stx1, stx2, eae and hly. Abstract Many bacteria including Escherichia coli can enter into the viable but non‐culturable (...
Tue, 08/18/2020 - 22:12
Vibrio parahaemolyticus is one of the most hazardous pathogens causing seafood‐borne diseases in the southern Fujian coast, China. From June to October 2016, a total of 250 samples were collected from retail markets in the Xiamen, Quanzhou, and Zhangzhou regions. Seventy‐seven V. parahaemolyticus isolates were identified using polymerase chain reaction (PCR). Then, molecular typing was performed...
Tue, 08/18/2020 - 22:12
Aspergillus, Penicillium, Fusarium, and Alternaria species which produce toxic metabolites, create a big problem in terms of the production of reliable human food and animal feeds. This study was conducted to determine the mycotoxin contents of foodstuff collected uniformly in each year of 2017, 2018, and 2019 in Şebinkarahisar, Turkey by enzyme‐linked immunosorbent assay. The highest total...
Tue, 08/18/2020 - 22:12
In this study, the effectiveness of ACP in inactivating Aspergillus flavus inoculated to military rations snack using response surface methodology (RSM) was investigated. Additionally, the effect of this treatment on the total count and yeast–mold count, as well as some quality properties of military rations snack, was examined. RSM was applied to study the voltage effects (5–15 kV), the distance...
Thu, 08/13/2020 - 22:12
A new selective broth, SSEL, was developed for simultaneous enrichment of S. enterica, S. aureus, E. coli O157: H7, and L. monocytogenes, optimizing by single factor experiments and a L9 (34) orthogonal test. Developed SSEL broth not only enriched the target pathogens to 5 log10 CFU/ml after 18 hr incubation at 37°C with 10–100 CFU/mL of inoculation concentration, but also could successfully...
Tue, 08/11/2020 - 22:12
Isochoric freezing, different from isobaric (conventional) freezing, has a superior capability to reduce foodborne pathogens (a) photo and (b) schematic of a prototypical isochoric chamber. Abstract Isochoric freezing, different from isobaric (conventional) freezing, allows for storage below freezing temperatures without significant damage from ice formation. While several types of tissues have...
Mon, 08/10/2020 - 22:12
We have developed a visual on‐site detection of African swine fever by the denaturation bubble‐mediated strand exchange amplification with colorimetric detection strategy. Combined with rapid extraction, the entire detection process takes only 50 min and only requires a portable metal bath. African swine fever virus was successfully detected in eight different tissues and seven types of pig feed...
Fri, 08/07/2020 - 22:12
Freshly harvested Bambara groundnut seed obtained from rural framers in the Ehlanzeni District Municipality of the Mpumalanga province, South Africa does not have detectable levels of mycotoxins. Freshly harvested Bambara groundnut seed were naturally contaminated with mycotoxin producing isolates from the genera Aspergillus and Fusarium. Abstract Freshly harvested Bambara groundnut (BGN) is...
Thu, 08/06/2020 - 22:11
Antifungal effects of smoldering plants are studied in vitro and pistachio nuts. The smoke of the cinnamon plant had an in vitro fungicidal effect in the PDA medium. No aflatoxin was observed in pistachios treated by cinnamon bark smoke. A reliable method is introduced for pistachio farmers to provide safe products. Abstract Aflatoxin (AF) contamination of the Iranian exporting pistachio has...
Wed, 08/05/2020 - 22:11
The image of a probe thermometer as a nudge tool significantly improved compliance with and control of the critical control points. Citrus scent with a short inscription significantly improved the hygiene behavior of the food handlers at all critical stages of food preparation. Pictures and short inscriptions turned out to be the most effective nudge tools to improve the food handlers' hygiene...
Tue, 08/04/2020 - 22:11
This study investigated antimicrobial efficacy of trans‐cinnamaldehyde (TC) and chitosan (CH) along with high pressure processing (HPP) against Cronobacter sakazakii in infant formula. All treatments exhibited varying degrees of C. sakazakii reductions up to undetectable levels. Remarkable deformation and damage in C. sakazakii cells were observed by transmission electron microscopy after the...
Tue, 08/04/2020 - 22:11
Two monoclonal antibodies (mAbs) specific to Salmonella were produced from a mouse immunized with a combination of heat‐killed whole‐cell and formalin‐fixed Salmonella enterica serovar Enteritidis (S. Enteritidis) as the antigens. The mAb SE‐13D bound specifically only to S. Enteritidis, while mAb Sal‐06G bound to various members of the Salmonella serogroups B, C, D, E, and I. Neither mAb...
Tue, 07/28/2020 - 22:11
The developed miniaturized test (s) rapidly detects Escherichia coli/coliforms colorimetrically utilizing specific enzyme substrates and selective agents incorporated in media. High sensitivity and selectivity for target bacteria. A total of 139 milk samples were tested for presence of E.coli/coliforms using developed test (s). Abstract Current investigation was aimed to develop colorimetric...
Sat, 07/25/2020 - 22:09
Lateral‐flow immunochromatographic assay (LFIA) was performed on pastry samples incubated for different time‐periods and the degree of coliform contamination evaluated. Pastries contaminated with ~5.0 log10 (cfu/g) coliforms showed LFIA positivity by 3 hr culture, whereas pastries contaminated with 2.0 log10 (cfu/g) coliforms showed LFIA positivity by 9 hr culture. The degree of coliform...
Wed, 07/15/2020 - 22:12
NaHB4, Te powder, CdCl2 and mercaptopropionic acid under a dark place were synthesized to get CdTe quantum dots (QDs) precursors, the precursors were furtherly reacted with zinc acetate and sulfur acetamide to obtain CdTe/ZnS QDs. The CdTe/ZnS QDs were covalently coupled with streptavidin by EDC•HCL and succinimide in the next step. The CdTe/ZnS QDs and Antibody were linked by avidin on the CdTe/...
Wed, 07/08/2020 - 22:11
Efficacy of N‐Halamine on Foodborne Pathogens Abstract A major concern of the cattle industry is cross‐contamination of meat with pathogens. Cattle are exposed to fecal material, mud, and other contaminants which harbor pathogens that can be shed onto meat and meat processing equipment. Due to increased chances of meat contamination during processing, new antimicrobial formulations for carcass...
Sat, 06/27/2020 - 22:10
The antimicrobial calcium‐alginate‐based films containing the self‐microemulsifying (SME) thyme essential oil (TEO) formulations were fabricated successfully. A Ca‐alginate film containing nano‐emulsified TEO as well as a neat Ca‐alginate film were considered as the controls. The SME films released the TEO completely within 155 min and inhibited the growth of S. aureus and E. coli in in vitro...
Fri, 06/19/2020 - 22:10
Nyjer and flax seeds are important oilseeds that are used for both human and animal consumption. Aspergilluscarbonariusproduced ochratoxin A (OTA) up to 271 μg/kg on ground nyjer seeds incubated with 0.98 awat 20°C, while A. carbonarius produced the highest OTA (146 μg/kg) on ground flax seeds incubated with 0.94 awat 20°C. Overall, ground nyjer seed is better than flax seed to support growth and...
Tue, 06/09/2020 - 22:11
Campylobacter is the most common cause of bacterial gastroenteritis worldwide and the most frequently reported foodborne pathogen in the European Union (EU). The predominant source of infection is poultry. The reduction of Campylobacter spp. in the poultry meat chain in the EU can only be achieved with an integrated meat safety assurance approach. Abstract Campylobacter is the most common cause...
Fri, 06/05/2020 - 03:28
Microbial inspections were conducted at a hospital kitchen in both Vietnam and JapanThe mean general viable bacteria found on cookware and equipment in the Vietnamese hospital kitchen was significantly higher than in the Japanese kitchen.Coliform and E. coli were detected in cooked foods in the Vietnamese, but not the Japanese, hospital kitchen. Abstract This study aimed to assess food safety in...
Thu, 05/28/2020 - 03:29
Antimicrobial effect of oregano and thyme essential oils (1%) in minced pork was assessed after storage at 3°C/7 days in a vacuum. The inhibition of S. Typhimurium depended mainly on the fat content (logarithmic dependence) and to a lesser degree on the pH, whereas pH was not found to be a significant predictor in the case of L. monocytogenes. The results show that only lean minced meat (up to 5...
Thu, 05/28/2020 - 03:29
Royal jelly (RJ) and propolis were able to inhibit the growth of Aspergillus parasiticus with minimum inhibitory concentrations (MICs) of 3,200 and 100 μg/ml, respectively. Combination of RJ and propolis resulted in synergistic interaction with MICs of 200 and 25 μg/ml, respectively, and a fractional inhibitory concentration index of 0.312. RJ and propolis were able to down‐regulate the...
Mon, 05/25/2020 - 03:26
A novel selective enrichment broth (SLB) combined with a loop‐mediated isothermal amplification (LAMP) kit was developed for inspection of Salmonella in ready‐to‐eat fruits and vegetables. A novel selective enrichment broth was developed for the inspection of Salmonella. The recovery of injured Salmonella in SLB was superior to Selenite Cystine Broth. The growth of Salmonella and inhibitory non‐...
Fri, 05/08/2020 - 03:27
The main compounds of LC‐EO were citral and nerol. In the present study, gram‐negative bacteria had greater sensitivity to LC‐EO than gram‐positive bacteria. LC‐EO could lead to damage to the cell wall and membrane, resulting in the leakage of cell contents, such as electrolytes and nucleic acids, and eventual cell death. Abstract Litsea cubeba essential oil (LC‐EO) has been reported as...
Thu, 05/07/2020 - 03:26
PDT is an emerging new antimicrobial technology, which uses a combination of photosensitizers and light. This study, for the first time, demonstrated that ERY (Red No. 3)‐mediated PDT can be an alternative antimicrobial treatment strategy to the conventional treatments used in tomato juice. Abstract The objectives of this study were to evaluate the efficacy of erythrosine B (ERY, Red No. 3)‐...

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