Journal of Separation Science

The Research Publications track research that is published across national and international peer-reviewed journals. The most recent articles are available ahead of print and searchable by Journal Title, Subject, USDA Grant Funding Agencies or FDA Grant Funding Agencies. The research publications are tracked across five subject areas: Bacterial Pathogens, Chemical Contaminants, Natural Toxins, Parasites, and Viruses.

Tue, 08/11/2020 - 22:11
A new analytical method based on capillary LC with diode array detection has been developed for the simultaneous quantification of seven neonicotinoid insecticides commercially available (imidacloprid, thiacloprid, clothianidin, thiamethoxam, acetamiprid, nitenpyram and dinotefuran) in honey samples. The separation was achieved in a Zorbax XDB‐C18 column (150 × 0.5 mm id, 5 μm), with a mobile...
Tue, 08/04/2020 - 22:10
This study ascertained the viability of a portable liquid chromatograph, operating at low μL/min flow, for the analysis of seized drugs at remote sites as well as in laboratory settings. Synthetic cathinones were screened using dual capillary columns in series, C8 and biphenyl, with on‐column ultraviolet detection at 255 nm and 275 nm. The relative retention times of the two columns in series and...
Thu, 07/30/2020 - 22:10
In this work, graphene oxide‐hybridized high internal emulsion polymers with crosslinking and open‐cell structure was prepared and applied for separation and enrichment of estrogens. The prepared graphene oxide‐hybridized high internal emulsion polymer monoliths had hydrophobicity, porosity and stability, which were just obtained by one step in‐situ emulsion polymerization of 2‐ethylhexyl...
Thu, 07/30/2020 - 22:10
A method for separation and determination of 32 fentanyl‐related substances, including seven sets of isomeric fentanyl analogues, was developed using ultra‐high‐performance liquid chromatography coupled with quadrupole‐orbitrap high‐resolution mass spectrometry. The collision energy, chromatographic column and mobile phase were optimized. All compounds were efficiently flushed out of a universal...
Thu, 07/30/2020 - 22:10
An analytical method for the determination of 6 emerging derivatives or metabolites together with 25 common macrolides antibiotics in milk by ultra‐performance liquid chromatography quadrupole/electrostaticfield orbitrap mass spectrometry was established. The samples were purified with optimized Quick, Easy, Cheap, Effective, Rugged, Safe methods. The amounts of primary‐secondary amine, C18 and...
Wed, 07/29/2020 - 22:09
Peptide sequencing is critical to the quality control of peptide drugs and functional studies of active peptides. A combination of peptidase digestion and mass spectrometry technology is common for peptide sequencing. However, such methods often cannot obtain the complete sequence of a peptide due to insufficient amino acid sequence information. Here, we developed a method of generating full...
Fri, 07/24/2020 - 22:10
A magnetic covalent organic framework material was synthesized with a core‐shell structure using a simple solvothermal method. It was prepared with Fe3O4 as the magnetic core, covalent organic framework as the shell, which synthesized from 1,3,5‐triformylphloroglucinol and p‐phenylenediamine by Schiff base reaction. The transmission electron microscope, Fourier transform infrared spectroscopy,...
Thu, 07/23/2020 - 22:10
A sample pretreatment method based on the combination of a three‐phase solvent extraction system and deep eutectic solvent‐based dispersive liquid–liquid microextraction has been introduced for the extraction of four organochlorine pesticides in cocoa samples before their determination by gas chromatography–electron capture detection. A mixture of sodium chloride, acetonitrile, and potassium...
Tue, 07/21/2020 - 22:09
Although an efficient and stable fiber coating is essential for the development of solid‐phase microextraction technique, it remains a challenging prospect. Herein, an inorganic nanocomposite material Ag2S@ZnS was prepared and used as a coating for fibers to detect polycyclic aromatic hydrocarbons in water samples in combination with a GC with flame ionization detector. Compared with a single ZnS...
Tue, 07/14/2020 - 22:11
In order to enhance the extraction performance, a mesoporous silica was modified with ordered mesoporous carbon for solid‐phase microextraction. Three stainless steel wires coated with the mesoporous material were placed in a polyetheretherketone tube for getting an extraction tube. The tube was coupled to high performance liquid chromatography with diode array detector, and the online analysis...
Fri, 07/10/2020 - 22:10
Levetiracetam is an antiepileptic drug for the treatment of psychiatric patients. In this study, a selective, straightforward, and rapid online heart‐cutting LC method was developed for the therapeutic drug monitoring of levetiracetam. This method allows for the determination of levetiracetam in human plasma without complex sample preparation. The mobile phases consisted of 30 mM aq. ortho‐...
Fri, 07/10/2020 - 22:10
Drug resistance is one of the main problems of cancer treatment. For this reason, combination therapy is commonly used for years. The combination of a chemotherapeutic, carboplatin, and the epigenetic drug decitabine is a new approach to modulate drug resistance. Nanoparticulate systems can overcome the drawbacks associated with the drug combinations. An analytical method that can detect and...
Tue, 07/07/2020 - 22:09
Emerging drugs usually mimic the effects of traditional drugs, but are not always controlled due to the chemically altered structures. Positional isomers of emerging drugs are challenging to analyze because they challenge the separation and detection techniques commonly employed by forensic laboratories. The utility of silica hydride (SiH) stationary phases for the ultra high performance liquid...
Mon, 07/06/2020 - 22:10
This research is an application of fiber‐in‐tube solid phase microextraction followed by HPLC‐UV for extraction and determination of trace amounts of bisphenol A. Nano magnetic Fe3O4 was formed on the surface of polypropylene porous hollow fibers to increase the surface area and then it was coated with polystyrene. The introduction of polystyrene improves the surface hydrophobicity and is an...
Mon, 07/06/2020 - 22:10
CE is a modern separation technique characterized by many benefits, which qualify it also for enzyme assays and the study of enzyme kinetics during drug development. Homogenous or heterogenous approaches can be followed for the enzymatic incubation. In this study, an immobilization procedure of aldehyde oxidase on magnetic particles was developed considering their integration with CE. A number of...
Fri, 07/03/2020 - 22:10
Detecting pesticide residues in human serum is a challenging process. This study developed and validated a method for the extraction and recovery of residues of multiple classes of pesticides from serum using one reagent. Salt‐assisted acetonitrile extraction and HPLC with quadrupole time of flight MS/MS were used to quantitate 34 pesticides classified in nine groups of chemicals in human serum...
Fri, 07/03/2020 - 22:10
The study aimed to establish a automated versatile sample preconcentration method based on the modified immunomagnetic beads, which was utilized to enrich for aflatoxin B1 from the matrices. The critical main parameters affecting the extraction efficiency, such as usage amount of immunomagnetic beads, reaction time, elution time, blending way was investigated. Under the optimized conditions, the...
Wed, 06/24/2020 - 22:09
Bilobetin, a natural compound extracted from Ginkgo biloba, has various pharmacological activities such as anti‐oxidation, anti‐cancer, anti‐bacterial, anti‐fungal, anti‐inflammatory, anti‐viral, and promoting osteoblast differentiation. However, few studies have been conducted and there are no reports on its metabolites owing to its low content in nature. In addition, it has been reported to...
Wed, 06/17/2020 - 22:10
A novel, facile, and robust strategy was proposed to increase the pore size and mechanical strength of cryogels. By mixing the monomers of acrylamide and 2‐hydroxyethyl methacrylate as the precursor, a monolithic copolymer cryogel with large interconnected pores and thick pore walls was prepared. Hydrogen bonding between the two monomers contributed to the entanglement and aggregation of the...
Mon, 06/15/2020 - 22:09
Roukou Wuwei pills is one of the most commonly used Mongolian medicinal prescriptions and historically used for the treatment of depression. This research aimed to illustrate the metabolic characteristic of Roukou Wuwei pills in vivo. To address this objective, an ultra high performance liquid chromatography with quadrupole time‐of‐flight mass spectrometry method based metabonomics approach was...
Wed, 06/10/2020 - 22:11
In this work, a simple, fast, sensitive, and environmentally friendly method was developed for preconcentration and quantitative measurement of bisphenol A in water samples using GC‐MS instrument. The preconcentration approach, namely biosorption‐based dispersive liquid‐liquid microextraction with extractant removal by magnetic nanoparticles was performed based on the formation of microdroplet of...
Sun, 06/07/2020 - 22:10
A fast, green, sensitive, and accurate analytical method using HPLC couple with fluorescence detection, was established and validated for the simultaneous determination of amlodipine besylate and celecoxib in their recently approved fixed‐dose combination tablets (1:20). Separation of the two drugs was achieved on C18 reversed phase column (Thermo ODS Hypersil,4.6 × 250 mm, particle size 5μm)...
Sun, 06/07/2020 - 22:10
A mesoporous silica was functionalized by carbon nanotubes to enhance the extraction performance. The mesoporous material was coated on stainless steel wires, and three wires were inserted inside of a polyetheretherketone tube for in‐tube solid‐phase microextraction. The tube was coupled to high performance liquid chromatography with diode array detection to obtain online analytical system, then...
Sat, 05/30/2020 - 03:30
Graphene oxide has received extensive attention because of its unique properties and potential applications. In this study, magnetic nitrogen‐doped graphene was prepared by one‐step hydrothermal reaction using urea as the dopant and reductant, and ferroferric oxide nanoparticles were in‐situ deposited on the surface of the nanohybrids. The magnetic nitrogen‐doped graphene was characterized using...
Sun, 05/17/2020 - 03:25
In this work, various types of metal‐organic frameworks were synthesized, and their affinities towards buprenorphine were evaluated using dispersive solid‐phase extraction. The extracted buprenorphine was determined by ultra‐performance liquid chromatography‐ultraviolet detection system. The highest extraction recovery was observed by employing zeolitic imidazole framework‐67. Then, a facile and...
Mon, 05/11/2020 - 03:27
A new sol‐gel hybrid methyltrimethoxysilane‐chloropropyltriethoxysilane was prepared as sorbent for solid phase extraction. The extraction efficiency of the prepared sol‐gel hybrid methyltrimethoxysilane‐chloropropyltriethoxysilane were assessed by using three selected organophosphorus pesticides namely chlorpyrifos, profenofos and malathion. Gas chromatography‐mass spectrometry was used for...
Fri, 04/24/2020 - 03:24
In this study, the new and efficient three‐dimensional network porous aromatic frameworks materials called as Silica‐PAFs‐a, Florisil‐PAFs‐a, Silica‐PAFs‐b and Florisil‐PAFs‐b were first synthesized. The properties of materials were analyzed by five characterization methods. And they were used as adsorbents in pipette‐tip solid‐phase extraction for the effective determination of carbendazim and...
Mon, 04/13/2020 - 03:18
A novel solid‐phase microextraction fiber was synthesized by coating of stainless steel wire with polyoxomolybdate368/polyaniline as a sorbent aimed at extraction of amitriptyline, nortriptyline and doxepin as antidepressant drugs from urine and blood samples. The polyoxomolybdate368/polyaniline composite coating was carried out using electropolymerization process under constant potential. This...
Sun, 04/12/2020 - 03:18
Monitoring biological samples at trace levels of chemicals from anthropogenic actions such as pesticides, pharmaceuticals and hormones has become a very important subject. This work describes a method for the determination of 8 compounds of different chemical classes in human urine samples. Dispersive liquid‐liquid microextraction based on magnetic ionic liquids was used as the sample preparation...
Thu, 04/09/2020 - 03:20
Kang‐Ai injection, which is composed of Astragali Radix, Ginseng Radix, et Rhizoma, and kushenin, is extensively used in China as an adjuvant therapy for many types of cancer and chronic hepatitis B. In the present study, 47 herbal compounds (11 alkaloids, 8 astragalosides, and 28 ginsenosides), were detected in Kang‐Ai injection by ultra‐high‐performance liquid chromatography coupled to...
Mon, 04/06/2020 - 03:17
In the present work, a novel database of drug compounds and a rapid screening method based on ultra‐high performance liquid chromatography coupled to high resolution orbitrap mass spectrometry were developed and applied in the screening and identification of targeted and non‐targeted antihypertensive adulterants in dietary supplements and herbal medicines. The established screening database...
Fri, 04/03/2020 - 03:17
A solid phase microextraction with carbon nanospheres coated fiber coupled with gas chromatographic detection was established for the determination of eight polycyclic aromatic hydrocarbons (naphthalene, biphenyl, acenaphthene, fluorine, phenanthrene, anthracene, fluoranthene and pyrene) in water and soil samples. The experimental parameters (extraction temperature, extraction time, stirring rate...
Wed, 04/01/2020 - 03:17
Amphiphilic diblock copolymers consisting of a hydrophobic core containing a polymerized ionic liquid and an outer shell composed of poly(N‐isoprolylacrylamide) were investigated by CE and asymmetrical flow field flow fractionation. The polymerized ionic liquid comprised poly(2‐(1‐butylimidazolium‐3‐yl)ethyl methacrylate tetrafluoroborate) with a constant block length (n = 24), while the length...
Tue, 03/31/2020 - 03:18
ABSTRACT In this research, a new ultrasonic‐assisted dispersive micro solid‐phase extraction method based on N‐doped mesoporous carbon sorbent followed by high‐performance liquid chromatography equipped with diode array detector for trace measurement of 1‐hydroxypyrene as a metabolite of exposure to polycyclic aromatic hydrocarbons was optimized. Herein, the hard template method was used for the...
Tue, 03/31/2020 - 03:18
A novel ecofriendly, cost and time saving high performance thin layer chromatographic method was developed and validated for simultaneous determination of metoclopramide, ergotamine, caffeine and paracetamol in bulk and pharmaceutical formulation. The separation was carried out on silica gel plates, using ethyl acetate: ethanol: ammonia (9: 1: 0.1, by volume) as a developing system. Ultra violet...
Mon, 03/23/2020 - 03:19
Saxitoxin, which is one of the most typical paralytic shellfish poisoning toxins, ranks the highest intoxication rate of marine biological poisoning cases globally. Efficient clean‐up and extraction of saxitoxin from complex biological matrices are imperative for the analysis and concentration monitoring of the toxin when correlative poisoning cases happen. Herein, L‐cysteine modified magnetic...
Wed, 03/18/2020 - 03:18
Two HPLC‐MS/MS methods were developed and validated for the quantification of edaravone (method A) or taurine (method B) in human plasma. After protein precipitation, separations were achieved on an Ultimate XB‐C8 (2.1 × 50 mm, 3.0 μm) column for edaravone and a ZORBAX SB‐Aq column (2.1 × 100 mm, 3.5 μm) for taurine, respectively. The detection used electrospray ionization source via multiple...
Mon, 03/16/2020 - 03:17
ABSTRACT Pesticides have posed significant threats to aquatic ecosystems, yet little is known about their transformation products. The challenge is to simultaneously analyze various pesticides and transformation products in water as they have distinct physicochemical properties. A mix‐mode solid phase extraction method was established to simultaneously analyze current‐use pesticides and their...
Mon, 03/16/2020 - 03:17
Anabolic androgenic steroids are widely abused substances in sports doping. Their detection present limitations regarding the use of soft ion sources such as electrospray or atmospheric pressure chemical ionization by LC‐MS/MS. In the current study, a novel derivatization method was developed for the ionization enhancement of selected anabolic androgenic steroids. The proposed method aims at the...
Sun, 03/08/2020 - 04:17
In this study, a green mode of solidification of floating organic droplet–based dispersive liquid–liquid microextraction has been developed for the extraction of sixteen polycyclic aromatic hydrocarbons from honey samples before their determination by gas chromatography–mass spectrometry. In this method, an appropriate volume of menthol:decanoic acid deep eutectic solvent (as an extraction...
Wed, 03/04/2020 - 03:30
This paper describes the synthesis and application of a magnetic amino‐functionalized hollow silica‐titania microsphere as a new sorbent for magnetic dispersive micro‐solid phase extraction of selected pesticides in coffee bean samples. The sorbent was fully characterized by Fourier transforms infrared spectroscopy, field emission scanning electron microscopy, transition electron microscopy,...
Wed, 03/04/2020 - 03:30
An ultra‐performance liquid chromatography‐tandem mass spectrometric method has been developed for the highly sensitive and selective determination of regulated aflatoxins. The extraction of aflatoxins from baby food matrices were performed using liquid–liquid extraction procedure followed by immunoaffinity column cleanup. The higher sensitivity for the determination of target aflatoxins was...
Sat, 02/22/2020 - 03:18
Polyethylene glycols are synthetic polymers composed of repeating oxyethylene subunits, which have been known for non‐toxic, non‐immunogenic, non‐antigenic, good solubility in water and therefore approved for pharmaceutical applications. Recently, attachment or amalgamation of polyethylene glycols to therapeutic small molecules, peptides, proteins or nanoparticles has become a mature technology...
Fri, 02/14/2020 - 03:17
Polychlorinated biphenyls belong to a class of hazardous and environmental pollutants. GC separation and experimental relative retention time evaluation of these compounds on a poly (94% methyl/5% phenyl) silicone based capillary non‐bonded and cross‐linked column are time‐consuming and expensive. In this study, relative retention times were estimated using two‐dimensional images of molecules...
Wed, 02/12/2020 - 03:17
Sulfonamides in environmental water, food, and feed are a major concern for both aquatic ecosystems and public health, because they may lead to the health risk of drug resistance. Thus, numerous sensitive detection and rapid removal methodologies have been established. This review summarizes the sample preparation techniques and instrumental methods used for sensitive detection of sulfonamides....
Wed, 01/29/2020 - 03:30
A new version of magnetic solid phase extraction performed in a narrow bore tube has been proposed for the extraction and preconcentration of different pesticides from various vegetable and fruit juices followed by gas chromatography. A few mg of C8@SiO2@Fe3O4 nanoparticles are added into an aqueous sample solution placed in a narrow bore tube. The sorbent particles move down through the tube...
Mon, 01/27/2020 - 03:18
In this paper, porous sandwich structure Fe3O4 nanoparticles coated by polyhedral oligomeric silsesquioxanes and β‐cyclodextrin were prepared by surface polymerization using as the magnetic solid phase extraction adsorbent for the extraction and determination of carbaryl and carbofuran. The Fe3O4 nanoparticles coated with polyhedral oligomeric silsesquioxanes and β‐cyclodextrin were characterized...
Sat, 01/25/2020 - 03:17
A rapid and effective method was developed for the analysis of 39 pesticide residues in porcine meat with dual‐layer multi‐plug filtration cleanup and gas chromatography‐tandem mass spectrometry detection. The cleanup process was performed with columns packed with two layers, namely multi‐walled carbon nanotubes, C18 and anhydrous magnesium sulfate (MgSO4) as top layer, while mixture of florisil...
Thu, 01/16/2020 - 03:16
Despite aflatoxins contamination in feedstuff is a well‐known problem, and hence these residues are controlled in poultry products, there are scarce information regarding the presence of these toxic substances in aquaculture fish, facilities which use several feedstuff for fish breeding. A simple, rapid, and sensitive method has been therefore developed for aflatoxins (B1, B2, G1, and G2)...
Sat, 01/11/2020 - 03:16
This work provides a simple and rapid method for synthesis uniform simetryn imprinted nanoparticles, which can be used to pretreat the tested samples before detecting. A series of computational approach were employed for design simetryn‐imprinted polymer. Based on the conclusion of theoretical calculation, the simetryn imprinted nanoparticles were synthesized using simetryn as template,...

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