Journal of Separation Science

Wed, 11/27/2019 - 03:18
This report presents the first ultra‐high performance supercritical fluid chromatography diode array detector based assay for simultaneous determination of iridoid glucosides, flavonoid glucuronides, and phenylpropanoid glycosides in Verbena officinalis (Verbenaceae) extracts. Separation of the key metabolites was achieved in less than 7 min on an Acquity UPC2 Torus Diol column using a mobile...
Sat, 11/23/2019 - 03:17
In this study, a modified quick, easy, cheap, effective, rugged, and safe method combined with ultra high performance liquid chromatography‐tandem mass spectrometry was developed for the multiclass determination of 28 plant growth regulators in various fruits. Different extraction solvents and adsorbents, including primary secondary amine, octadecylsilyl, graphitized carbon black, and zirconia‐...
Sun, 11/17/2019 - 03:16
ABSTRACT Organophosphate esters used as flame retardants and plasticizers are ubiquitous contaminants in surface waters. Many studies indicate that these compounds are neurotoxicants, endocrine disruptors and may affect reproduction and development of aquatic organisms. Thus, analytical methods that allow accurate quantification of these contaminants at environmentally relevant concentrations are...
Thu, 11/14/2019 - 03:17
Three types of molecularly imprinted solid phase microextraction fibers were fabricated through sol‐gel method using diazinon, parathion‐methyl and isocarbophos as templates, respectively, and assembled together to construct a multifiber for analysis of organophosphorus pesticides in complex matrices. The multifiber provided large extraction capacity and high imprinting factor up to 3.89. In...
Wed, 11/13/2019 - 03:17
A simple, rapid, capillary zone electrophoresis method was developed and validated for the analysis the two of novel aminoalkanol derivatives (I) and (II) of 1,7‐diethyl‐8,9‐diphenyl‐4‐azatricyclo[5.2.1.02,6]dec‐8‐ene‐3,5,10‐trione, which were found in earlier studies as potential anticancer drugs. Samples were analyzed to demonstrate the specificity and stability indicating ability of the...
Thu, 11/07/2019 - 03:18
The efficiency of a molecularly imprinted polymer as a selective packing material for solid‐phase extraction of imatinib mesylate sorption was investigated. Molecularly imprinted polymer was prepared using N, N'‐methylenebisacrylamide, as a cross‐linker agent, N‐vinylcaprolactam as a thermo‐sensitive monomer, 1‐vinyl‐2‐pyrrolidone and methyl methacrylate as functional monomers,...
Thu, 11/07/2019 - 03:18
Despite substantial developments of extraction and separation techniques, isolation of natural products from natural sources is still a challenging task. Undoubtedly hybrid methods like LC‐NMR or LC‐MS made on‐line structure elucidation possible and provided impressive examples of natural product identification without prior isolation, however, in many cases the necessity to get the purified...
Tue, 11/05/2019 - 03:19
The first dispersive liquid liquid microextraction scheme followed by liquid chromatography‐post column derivatization for the determination of the antiviral drug – rimantadine – in urine samples is demonstrated. The effect of the type and volume of organic extraction solvent, type and volume of disperser solvent, sample pH, ionic strength, extraction time and centrifugation speed on the...
Sat, 11/02/2019 - 03:18
The triazine‐based organic polymers@SiO2 nanospheres were prepared and applied as an extraction coating onto stainless steel wires and the wires were filled into polyetheretherketone tube for in‐tube solid‐phase microextraction. Taking polycyclic aromatic hydrocarbons as targets, main factors affecting extraction performance of the tube were investigated through coupling to high performance...
Fri, 11/01/2019 - 03:18
Determination of methamphetamine is of great importance in the clinical and forensic laboratories because there are low dosages of drugs in the biological media and social problems created due to the methamphetamine consumption. Polymeric carbon based‐nano composites are reasonable candidates for dispersive solid phase extraction method due to facial and affordable synthesis process and high...
Wed, 10/23/2019 - 03:24
In reversed‐phase LC, tetracycline antibiotics yield broad and asymmetrical peaks, as a result of their ionic interaction with the anionic free silanol groups and metal ion present in the silica‐based stationary phases (commonly derivatized with C18 groups). These frequently encountered difficulties were absolutely eliminated based on dual effect when methanesulfonic acid was employed as an...
Tue, 10/22/2019 - 03:21
In this study, an effective speed‐regulated directly suspended droplet microextraction method was developed to condense pesticide residues from teas through dispersive solid‐phase extraction prior to analysis by GC–MS/MS. The extractant was intentionally dispersed into the sample solution in the form of globules through high‐speed agitation. This procedure increases the contact area between the...
Wed, 10/09/2019 - 03:21
In this study, we have developed a method to assess adenosine 5ʹ‐triphosphate by adsorptive extraction using surface adenosine 5'‐triphosphate‐imprinted polymer over polystyrene nanoparticles (412 ± 16 nm) for selective recognition/separation from urine. Molecularly imprinted polymer was synthesized by emulsion copolymerization reaction using adenosine 5'‐triphosphate as a template, functional...
Wed, 10/09/2019 - 03:21
A kind of molecularly imprinted polymers modified with biocompatible medium were prepared by suspension polymerization. The obtained hybrid materials were used as the adsorbents for the solid‐phase extraction of aflatoxins B1 in real samples. A structural analog of the target, 6‐methyl‐4‐phenylchroman‐2‐one was used as the pseudo‐template, owing to their lower toxicity and cheaper price compared...
Tue, 10/08/2019 - 03:21
Molecular imprinted polymers prepared by Pickering emulsion method with lignin as stable particle were used as dispersants in matrix solid phase dispersion, followed by ultra performance liquid chromatography‐tandem mass spectrometry analysis to research the degradation behavior of matrine in soil. The molecular imprinted polymers have regularly morphology and uniformly particles. Moreover, the...
Fri, 10/04/2019 - 03:20
ABSTRACT In this study, quick, easy, cheap, effective, rugged and safe combined with dispersive liquid‐liquid microextraction is developed for extraction of ten pesticides in complex sample matrices of water and milk. In this regard, effective factors of proposed extraction technique combined with gas chromatography‐flame ionization detector were designed, modeled and optimized using central...
Thu, 09/26/2019 - 03:16
Silk fibers were carbonized to develop a biomass carbon material as an adsorbent for solid‐phase microextraction. The surface structure of the carbonized silk fibers was characterized by scanning electron microscope, and the graphitization degree was determined by Raman spectrometer. After the carbonization under high temperature, the orderliness and structural regularity of carbon atoms on silk...
Mon, 09/23/2019 - 03:16
A novel and porous solid phase microextraction fiber was prepared by quick and simple galvanic displacement reaction and applied to the determination of some polycyclic aromatic hydrocarbons in sunflower oil. The parameters affecting the porosity and thickness of the fiber, and parameters affecting the extraction efficiency including the extraction time, temperature and ionic strength were...
Mon, 09/23/2019 - 03:16
The biomonitoring of hydroxy polycyclic aromatic hydrocarbons in urine, as a direct way to access multiple exposures to polycyclic aromatic hydrocarbons, has raised great concerns due to their increasing hazardous health effects on humans. Solid‐phase extraction is an effective and useful technique to preconcentrate trace analytes from biological samples. Here, we report a novel solid‐phase...
Mon, 09/23/2019 - 03:16
Ion chromatography coupled with mass spectrometry is an established technique for determination of ionic analytes, however, sophisticated buffer removal equipment is required to eliminate inorganic compounds from the eluate before introduction into the ion source of mass spectrometer. A standard high‐performance liquid chromatography coupled with tandem mass spectrometry setup using an ion...
Mon, 09/23/2019 - 03:16
The control of enantiomeric purity and determination of individual enantiomeric drug molecules remains the subject of importance for clinical, analytical, and regulatory purposes and to facilitate an accurate evaluation of the risks posed by them to human health. A large number of pharmaceuticals are marketed and administered as racemates. Etodolac is among such nonsteroidal anti‐inflammatory...
Mon, 09/23/2019 - 03:16
The present paper deals with the multivariate optimization of an extraction‐purification strategy for the determination of phytoestrogens (daidzein, genistein, coumestrol, formononetin, and biochanin A) in soy‐based meat substitutes by high performance liquid chromatography with tandem mass spectrometry. For a reliable quantitation of these new concerning compounds in such a complex matrix,...
Mon, 09/23/2019 - 03:16
A systematic Quality by Design approach was employed for developing an isocratic reversed‐phase liquid chromatographictechniquefor the estimation of ropinirole hydrochloride in bulk drug and pharmaceutical formulations. LiChrospher RP 18‐5 Endcapped column (25 cm × 4.6 mm i.d.) at ambient temperature (25±2°C) was used for the chromatographic separation of the drug. The screening of factors...
Mon, 09/23/2019 - 03:16
The harmful health effects caused by phthalic acid esters have been supported from the increasing scientific evidence, developing the efficient methodologies to monitor the levels of phthalic acid esters in various foods become especially important from the aspects of human exposure assessment and their migration mechanistic understanding. In this study, quantitative structure‐retention...
Mon, 09/23/2019 - 03:16
We herein presented a mesoporous cellular foam solid‐phase microextraction coating that showed highly sensitive recognition for weakly polarity polychlorinated biphenyls in water samples. The mesoporous cellular foam coater fiber was for the first time prepared by a simple sol‐gel method. The main experimental parameters including extraction temperature, extraction time, desorption time, stirring...
Mon, 09/23/2019 - 03:16
Steroid hormones as endocrine disrupting compounds can interfere with the functioning of hormonal systems of organisms and thus affect the health and reproduction of humans and wildlife. Unfortunately, these types of harmful endocrine disrupting compounds have been found in a variety of environmental samples at very low concentrations. Therefore, a simple, fast, and efficient method for...
Mon, 09/23/2019 - 03:16
Xiaojin Capsule, a classic traditional Chinese medicine formula, has been used to treat mammary cancer, thyroid nodules, and hyperplasia of the mammary glands. However, its systematic chemical information remained unclear, which hindered the interpretation of the pharmacology and the mechanism of action of this drug. In this research, an ultra high performance liquid chromatography coupled with a...
Mon, 09/23/2019 - 03:16
ABSTRACT The ripened seeds of Strychnos nux‐vomica L. have been extensively used as herbal medicines in Asian countries. Dihydroindole‐type alkaloids are not only the active constituents but also the toxicants in Strychnos. However, the simultaneous determination of these alkaloids in both crude and processed Semen Strychni is still lacking. The present study represents the first quantitation and...
Mon, 09/23/2019 - 03:16
Here, a two‐stage column separation scheme is developed for the concomitant isolation of Sr, Pb, Nd, and Hf from geological samples. The first column, which consists of three resin layers (AG50W‐X8 ion exchange resin + Ln specific resin + Sr specific resin), separates the high field strength element + rare earth element, Sr and Pb from the matrices. Subsequently, Nd and Hf are further separated...
Mon, 09/23/2019 - 03:16
Low potency corticosteroid betamethasone valerate and vitamin‐A tazarotene are used in combination for effective treatment of psoriasis. There is no robust HPLC analytical technique available for simultaneous estimation of betamethasone valerate and tazarotene in conventional and nanocarriers based formulations. The simple, accurate, robust isocratic HPLC method was developed for simultaneous...
Mon, 09/23/2019 - 03:16
This research deals with development of a stability‐indicating HPLC method for simultaneous determination of amprolium hydrochloride and ethopabate. To the best of our knowledge, no comprehensive stability‐indicating method has been reported for analysis of this mixture. Separation was achieved using Kromasil cyano column with gradient elution of the mobile phase composed of sodium hexane...
Mon, 09/23/2019 - 03:16
Driven by the significant need for characterization of the chemical speciation of arsenic in food, this work developed a method for rapid determination of four common arsenic species, namely, arsenite, arsenate, monomethyl arsenic acid, and dimethyl arsenic acid, in vegetables using microwave‐assisted extraction, followed by detection with high‐performance liquid chromatography hyphenated to...
Mon, 09/23/2019 - 03:16
The aim of this work was to develop an efficient method for the selective extraction and analysis of fenoxycarb, a carbamate pesticide, in mussel samples using a molecularly imprinted solid‐phase extraction device. The optimization of molecularly imprinted polymer synthesis was performed using the experimental design under the response surface methodology approach. A fast rebinding study and...
Mon, 09/23/2019 - 03:16
Nano‐molybdenum trioxide was prepared from nano‐molybdenum disulfide by simple firing in muffle furnace. Nano‐molybdenum trioxide was used as the extraction coating on the stainless steel wire. Four wires were filled in a polyetheretherketone tube to get an extraction tube. The tube was connected to the six‐port valve of a high performance liquid chromatograph, and the online analysis system was...
Mon, 09/23/2019 - 03:16
Alarelin, a gonadotropin‐releasing hormone analogue, is widely used in China for the treatment of endometriosis and uterine leiomyoma. In order to investigate its pharmacokinetic behavior and support the preclinical application of new formulations, we have developed a novel and highly selective bioanalytical method to determine alarelin in rat plasma based on liquid chromatography tandem mass...
Mon, 09/23/2019 - 03:16
A high‐throughput method based on ultrasonic‐assisted extraction, 96‐well plate thin‐film microextraction was established to determinate 18 antibiotics in animal feed. In this method, the extraction was implemented by ultrasonic‐assisted extraction for 30 min with disodium ethylenediaminetetraacetic acid‐McIlvaine buffer (pH 5) containing 6% sodium chloride w/v, purified by thin‐film...