PLoS One

Sat, 07/31/2021 - 08:01
by Hakan Adanacioglu The main purpose of this study is to determine the factors that motivate consumers who shop at farmers’ markets. The data for this study were gathered from questionnaires of 363 consumers from eight farmers’ markets in seven districts of Izmir province, Turkey. To reveal the consumer profile of the farmers’ markets examined in this study, consumer segments were determined...
Fri, 07/30/2021 - 08:01
by Xing Gao, Junping Tian, Zheng Huo, Yanbin Wu, Chenxi Li Heavy metal contamination in soil is an important factor affecting the determination of safe redevelopment methods for industrial and mining land. In this paper, the soil environment of a typical mining city in northern China was taken as the research object, 148 surface soil samples were collected and the contents of heavy metals were...
Thu, 07/29/2021 - 08:02
by Joseph Okotto-Okotto, Weiyu Yu, Emmah Kwoba, Samuel M. Thumbi, Lorna Grace Okotto, Peggy Wanza, Diogo Trajano Gomes da Silva, Jim Wright Water safety planning is an approach to ensure safe drinking-water access through comprehensive risk assessment and water supply management from catchment to consumer. However, its uptake remains low in rural areas. Participatory mapping, the process of map...
Sat, 07/24/2021 - 08:04
by Giulia Vecchiotti, Sabrina Colafarina, Massimo Aloisi, Osvaldo Zarivi, Piero Di Carlo, Anna Poma The potential risks of environmental nanoparticles (NPs), in particular Polystyrene Nanoparticles (PNPs), is an emerging problem; specifically, the interaction of PNPs with intestinal cells has not been characterized so far. The mechanism by which polystyrene particles are transferred to humans has...
Sat, 07/24/2021 - 08:04
by Chi-Chun Huang, Chih-Ming Liang, Ting-I Yang, Jiann-Long Chen, Wei-Kuang Wang Anthropogenic activities accompanied by heavy metal waste threaten the environment. Heavy metal pollution alters the soil microbial community composition, and the microorganisms that adapt to this stress increase in abundance. The remediation process of contaminated soil not only reduces the concentration of heavy...
Sat, 07/24/2021 - 08:04
by Soha El-Shaer, Shaymaa H. Abdel-Rhman, Rasha Barwa, Ramadan Hassan Over the past decades, Escherichia coli (E. coli) have acquired extensive resistance to antibiotics; especially β- lactams. This study aimed to investigate the frequency of Extended-spectrum β-lactamase (ESBL) and carbapenemase producers among E. coli isolates and their correlation with serotypes, phylogenetic background, and...
Thu, 07/22/2021 - 08:11
by Folake Temitope Fadare, Anthony Ifeanyi Okoh Globally, there is an increasing occurrence of multidrug-resistant (MDR) Enterobacteriaceae with extended-spectrum β-lactamases (ESBLs) and/or plasmid-encoded AmpC (pAmpC) β-lactamases in clinical and environmental settings of significant concern. Therefore, we aimed to evaluate the occurrence of ESBL/pAmpC genetic determinants, and some essential...
Wed, 07/21/2021 - 08:02
by Simeon Cadmus, Samson Polycarp Salam, Hezekiah Kehinde Adesokan, Kelechi Akporube, Fiyinfoluwa Ola-Daniel, Emmanuel Jolaoluwa Awosanya Brucellosis and Q fever are neglected zoonoses of global health importance, with unknown true prevalence in occupationally vulnerable settings, partly due to misdiagnosis for other febrile conditions and poor access to primary health care. We examined the...
Sat, 07/17/2021 - 08:01
by Adharsh Rajasekar, Charles K. S. Moy, Stephen Wilkinson, Raju Sekar Microbially Induced Carbonate Precipitation (MICP) is currently viewed as one of the potential prominent processes for field applications towards the prevention of soil erosion, healing cracks in bricks, and groundwater contamination. Typically, the bacteria involved in MICP manipulate their environment leading to calcite...
Thu, 07/15/2021 - 08:01
by Chinwe Juliana Iwu-Jaja, Anelisa Jaca, Ishmael Festus Jaja, Portia Jordan, Phelele Bhengu, Chidozie Declan Iwu, Joseph Okeibunor, Humphrey Karamagi, Prosper Tumusiime, Walter Fuller, Ali Ahmed Yahaya, Charles Wiysonge, Laetitia Gahimbare Introduction Antimicrobial resistance (AMR) constitutes a significant threat to global health and food security, typically associated with high morbidity and...
Tue, 07/13/2021 - 08:01
by Madiha Khadim Hussain, Abida Aziz, Hafiza Mamona Allah Ditta, Muhammad Farooq Azhar, Ahmed M. El-Shehawi, Sajjad Hussain, Noman Mehboob, Mubshar Hussain, Shahid Farooq Cadmium (Cd) is a widespread heavy metal, which commonly exert negative impacts on agricultural soils and living organisms. Foliar application of seed water extract of black cumin (Nigella sativa L.) can mitigate the adverse...
Fri, 07/09/2021 - 08:01
by Maria N. Ayala, Denise Y. P. Henriques The ability to switch between different visuomotor maps accurately and efficiently is an invaluable feature to a flexible and adaptive human motor system. This can be examined in dual adaptation paradigms where the motor system is challenged to perform under randomly switching, opposing perturbations. Typically, dual adaptation doesn’t proceed unless each...
Sat, 07/03/2021 - 08:01
by Iftekhar Bin Naser, Tushar Ahmed Shishir, Shah Nayeem Faruque, M. Mozammel Hoque, Anamul Hasan, Shah M. Faruque Prevalence of toxigenic Vibrio cholerae O1 in aquatic reservoirs in Bangladesh apparently increases coinciding with the occurrence of seasonal cholera epidemics. In between epidemics, these bacteria persist in water mostly as dormant cells, known as viable but non-culturable cells (...
Fri, 07/02/2021 - 08:01
by Thanawat Sridapan, Wanida Tangkawsakul, Tavan Janvilisri, Taradon Luangtongkum, Wansika Kiatpathomchai, Surang Chankhamhaengdecha Development of a simple, rapid and specific assay for the simultaneous detection of Campylobacter spp. and Salmonella spp. based on duplex loop-mediated isothermal amplification (d-LAMP), combined with lateral-flow biosensor (LFB) is reported herein. LAMP amplicons...
Fri, 07/02/2021 - 08:01
by Xiaoying Wu, Bei Wu, Yue Li, Xiue Jin, Xiliang Wang Enterococci have the dual characteristics of being opportunistic pathogens and promising probiotics. The isolation from patients of CDC PNS-E2, a newly described Enterococcus species Enterococcus sanguinicola, may pose potential hazards. Enterococcus thailandicus from fermented sausage is a senior subjective synonym of E. sanguinicola. In...
Thu, 07/01/2021 - 08:02
by Ha Thi Nguyen, Thien-Y Vu, Tikam Chand Dakal, Bhanupriya Dhabhai, Xuan Hong Quan Nguyen, Vinay Bharadwaj Tatipamula In the present study, in silico predictions and molecular docking were performed on five clerodane diterpenes (1–5) from Polyalthia longifolia seeds to evaluate their potential as xanthine oxidase (XO) inhibitors. The initial screening was conducted by target prediction using...
Thu, 07/01/2021 - 08:02
by Tae-Hwan Jung, Kyoung-Sik Han An imbalanced dietary intake is associated with alteration of intestinal ecosystem. We investigated the impact of imbalanced diets on colonic microbiota, concentrations of short chain fatty acid in colonic digesta and serum immunoglobulins (Igs) of growing rats. Compared to the control diet, consuming diets high in fat, sucrose, or processed meat, or low in iron,...
Fri, 06/25/2021 - 08:01
by Shaimaa F. Mouftah, José F. Cobo-Díaz, Avelino Álvarez-Ordóñez, Menattallah Elserafy, Nehal A. Saif, Asmaa Sadat, Ayman El-Shibiny, Mohamed Elhadidy Campylobacter species are one of the most common causative agents of gastroenteritis worldwide. Resistance against quinolone and macrolide antimicrobials, the most commonly used therapeutic options, poses a serious risk for campylobacteriosis...
Thu, 06/24/2021 - 08:02
by Lamia M. El-Samad, Saeed El-Ashram, Dalia A. Kheirallah, Karolin K. Abdul-Aziz, Noura A. Toto, El Hassan M. Mokhamer The present research aims to evaluate the impact of industrial processes and anthropogenic activities on the beetle Pimelia latreillei inhabiting the polluted site at Zawya Abd El- Qader, Alexandria, Egypt. Beetles were collected from the vicinity of five factories. The...
Thu, 06/24/2021 - 08:02
by Chee-Hoo Yip, Sobina Mahalingam, Kiew-Lian Wan, Sheila Nathan Prodigiosin, a red linear tripyrrole pigment, has long been recognised for its antimicrobial property. However, the physiological contribution of prodigiosin to the survival of its producing hosts still remains undefined. Hence, the aim of this study was to investigate the biological role of prodigiosin from Serratia marcescens,...
Tue, 06/22/2021 - 08:02
by Megan Fay, Joelle K. Salazar, Padmini Ramachandran, Diana Stewart Metagenomic analysis of food is becoming more routine and can provide important information pertaining to the shelf life potential and the safety of these products. However, less information is available on the microbiomes associated with low water activity foods. Pine nuts and sesame seeds, and food products which contain these...
Fri, 06/18/2021 - 08:02
by Ho Jae Lim, Eun-Rim Kang, Min Young Park, Bo Kyung Kim, Min Jin Kim, Sunkyung Jung, Kyoung Ho Roh, Nackmoon Sung, Jae-Hyun Yang, Min-Woo Lee, Sun-Hwa Lee, Yong-Jin Yang Classification of clinical symptoms and diagnostic microbiology are essential to effectively employ antimicrobial therapy for lower respiratory tract infections (LRTIs) in a timely manner. Empirical antibiotic treatment without...
Sat, 06/12/2021 - 08:04
by Nuntita Singpanomchai, Yukihiro Akeda, Kazunori Tomono, Aki Tamaru, Pitak Santanirand, Panan Ratthawongjirakul Multidrug-resistant tuberculosis (MDR-TB) poses a serious threat to TB control. Early diagnosis and proper treatment are essential factors to limit the spread of the disease. The existing molecular tests for MDR-TB usually require specific instruments, steady power supply, and routine...
Sat, 06/05/2021 - 08:04
by Jiayou Liu, Jie Zhang, Sun Ha Kim, Hyun-Sook Lee, Enrico Marinoia, Won-Yong Song Brassica rapa is an important leafy vegetable that can potentially accumulate high concentrations of cadmium (Cd), posing a risk to human health. The aim of the present study was to identify cadmium detoxifying molecular mechanisms in B. rapa using a functional cloning strategy. A cDNA library constructed from...
Sat, 05/29/2021 - 08:04
by Coleman T. Turgeon, Karen A. Sanders, Piero Rinaldo, Dane Granger, Heather Hilgart, Dietrich Matern, Elitza S. Theel Background Dried blood spots (DBS) are an established specimen type for clinical testing given their low cost, ease of collection and storage, and convenient shipping capabilities through the postal system. These attributes are complementary to the expansion of SARS-CoV-2...
Thu, 05/27/2021 - 08:04
by Nai-xing Zhang, Yan Guo, Hui Li, Xue-Qin Yang, Chao-xian Gao, Chang-ye Hui Mercury exists naturally and mainly as a man-made pollutant in the environment, where it exerts adverse effects on local ecosystems and living organisms. It is important to develop an appropriate synthetic biological device that recognizes, detects and removes the bioavailable fraction of environmental mercury. Both...
Fri, 05/21/2021 - 08:02
by Walter Souza Santos, Rodrigo Gurgel-Gonçalves, Lourdes Maria Garcez, Fernando Abad-Franch Attalea palms provide primary habitat to Rhodnius spp., vectors of Trypanosoma cruzi. Flying from palms, these blood-sucking bugs often invade houses and can infect people directly or via food contamination. Chagas disease (CD) risk may therefore increase when Attalea palms thrive near houses. For example...
Wed, 05/19/2021 - 08:02
by Kiran A. Faryar, Rachel M. Ancona, Zachary Reau, Sheryl B. Lyss, Robert S. Braun, Todd Rademaker, Ryane K. Sickles, Michael S. Lyons Objective Multiple HIV outbreaks among persons who inject drugs (PWID) have occurred in the US since 2015. Emergency departments (EDs), recognized as essential venues for HIV screening, may play a unique role in identifying undiagnosed HIV among PWID, who...
Fri, 05/14/2021 - 08:02
by Shruti Koulgi, Vinod Jani, Mallikarjunachari Uppuladinne V. N., Uddhavesh Sonavane, Rajendra Joshi Drug repurposing studies targeting inhibition of RNA dependent RNA polymerase (RdRP) of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) have exhibited the potential effect of small molecules. In the present work a detailed interaction study between the phytochemicals from Indian...
Thu, 05/13/2021 - 08:04
by Bezalem Tesfaye Araya, Kirubel Eshetu Ali, Dereje Assefa Geleta, Saba Gebremichael Tekele, Kassu Desta Tulu Background The Abbott RealTime MTB is an assay for the detection of Mycobacterium tuberculosis (MTB) complex DNA from respiratory specimens in combination with the Abbott RealTime RIF/INH assay for the detection of genetic resistance markers for isoniazid (INH) and rifampicin (RIF) from...
Wed, 05/12/2021 - 08:02
by Mohamad Malik Al-adil Baharudin, Mohamad Syazwan Ngalimat, Fairolniza Mohd Shariff, Zetty Norhana Balia Yusof, Murni Karim, Syarul Nataqain Baharum, Suriana Sabri Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) have reached epidemic proportions globally. Therefore, there is an urgent need for a continuous supply of antibiotics to combat the problem. In this study,...
Fri, 05/07/2021 - 08:03
by Pavarist Bussabong, Tirawat Rairat, Niti Chuchird, Arunothai Keetanon, Putsucha Phansawat, Kanokwan Cherdkeattipol, Phongchate Pichitkul, Waraporn Kraitavin Isoquinoline alkaloids (IQs) from Macleaya cordata are promising natural products for enhancing the growth performance and overall health condition of farmed animals. The present study aimed to investigate the effects of two formulas of IQ...
Thu, 05/06/2021 - 08:02
by Tegan N. Clarke, Megan A. Schilling, Luca A. Melendez, Sandra D. Isidean, Chad K. Porter, Frédéric M. Poly Introduction While Campylobacter jejuni is a leading foodborne bacterial pathogen worldwide, it poses a particular risk to susceptible populations in low- and middle-income countries (LMICs). A capsule-conjugate vaccine approach has been proposed as a potential solution, but little...
Tue, 05/04/2021 - 08:02
by Xiaoping Wang, Fengzhi He, Limin Zhang, Ang Yu Two typical brominated flame retardants (BFRs), namely, tetrabromobisphenol A (TBBPA) and hexabromocyclododecane (HBCD), were persistent organic pollutants widely detected in various environmental media. This study aimed to successfully synthesize micro-nano-structured magnetite particles (MNMPs) with surface modification by citric acid molecules...
Tue, 05/04/2021 - 08:02
by Yaraymi Ortiz, Alam García-Heredia, Angel Merino-Mascorro, Santos García, Luisa Solís-Soto, Norma Heredia Adherence of bacteria to the human intestinal mucosa can facilitate their internalization and the development of pathological processes. Escherichia coli O104:H4 is considered a hybrid strain (enteroaggregative hemorrhagic E. coli [EAHEC]), sharing virulence factors found in...
Fri, 04/30/2021 - 08:02
by Ahmed Gaballa, Sriya Sunil, Etienne Doll, Sarah I. Murphy, Tyler Bechtel, Veronica Guariglia-Oropeza, Martin Wiedmann The foodborne pathogen Listeria monocytogenes is able to survive across a wide range of intra- and extra-host environments by appropriately modulating gene expression patterns in response to different stimuli. Positive Regulatory Factor A (PrfA) is the major transcriptional...
Wed, 04/28/2021 - 08:02
by Diana Luise, Mathilde Le Sciellour, Arnaud Buchet, Rémi Resmond, Charlène Clement, Marie-Noelle Rossignol, Deborah Jardet, Olivier Zemb, Catherine Belloc, Elodie Merlot This study describes the fecal microbiota from piglets reared in different living environments during the weaning transition, and presents the characteristics of microbiota associated with good growth of piglets after weaning....
Wed, 04/28/2021 - 08:02
by Annette Fagerlund, Sileshi Gizachew Wubshet, Trond Møretrø, Gesine Schmidt, Grethe Iren Borge, Solveig Langsrud The frequency of foodborne outbreaks epidemiologically associated with Listeria monocytogenes in fresh produce has increased in recent years. Although L. monocytogenes may be transferred from the environment to vegetables during farming, contamination of food products most commonly...
Tue, 04/27/2021 - 08:02
by Paolo Salazar-Mendoza, Ivan Peralta-Aragón, Ladislao Romero-Rivas, Jordano Salamanca, Cesar Rodriguez-Saona Lower elevations are generally thought to contain a greater abundance and diversity of insect communities and their natural enemies than higher elevations. It is less clear, however, how changes in seasons influence this pattern. We conducted a 2-year study (2013‒2014) in guava orchards...
Tue, 04/27/2021 - 08:02
by Rafael Vessecchi Amorim Zafalon, Mariana Pamplona Perini, Thiago Henrique Annibale Vendramini, Vivian Pedrinelli, Mariana Fragoso Rentas, Isabela Benelli Morilha, Lucas Ben Fiuza Henríquez, Renata Maria Consentino Conti, Marcio Antonio Brunetto Given the importance of using the vitamin-mineral supplements to guarantee the minimum nutritional recommendations for pets in homemade foods, and...
Sat, 04/24/2021 - 08:03
by Hongke Wu, Ming Wang, Jay D. Raman, Alicia C. McDonald Exposures to heavy metals have been linked to prostate cancer risk. The relationship of these exposures with serum prostate-specific antigen (PSA), a marker used for prostate cancer screening, is unknown. We examined whether total urinary arsenic, urinary dimethylarsonic acid, blood cadmium, blood lead, and total blood mercury levels are...
Sat, 04/17/2021 - 08:02
by Fenghua Zhu, Beibei Zhang, Lianqin Zhu Current methods for detection of mycotoxin in feed are time-consuming and tedious. An up-converting phosphor technology-based lateral flow (UPT-LF) assay system is a new emerging technique for analytes detection. The aim of this study was to compare the performance of UPT-LF, an enzyme-linked immunosorbent assay (ELISA) and high-performance liquid...
Thu, 04/15/2021 - 08:05
by Ashraf Mohabati Mobarez, Mohammad Khalili, Ehsan Mostafavi, Saber Esmaeili Background Coxiella burnetii is the causative agent of Q fever which is a highly infectious zoonotic disease. C. burnetii has become one of the most important causes of abortion in livestock, which can lead to widespread abortions in these animals. There are very limited studies on the prevalence of C. burnetii...
Wed, 04/14/2021 - 08:02
by Takashi Aoyama, Tomoko Kudo The Japanese Ministry of Health requires large-scale cooking facilities to use sodium hypochlorite aqueous solution (HYP) on food preparation tools, equipment, and facilities to prevent secondary contamination. This study aimed to compare the disinfecting effect of HYP and surfactant using adenosine triphosphate (ATP) swab testing on large-scale equipment and...
Tue, 04/13/2021 - 08:04
by Hiroko Enomoto, James Yeatts, Liliana Carbajal, B. Radha Krishnan, Jay P. Madan, Sandeep Laumas, Anthony T. Blikslager, Kristen M. Messenger There is no FDA approved therapy for the treatment of celiac disease (CeD), aside from avoidance of dietary gluten. Larazotide acetate (LA) is a first in class oral peptide developed as a tight junction regulator, which is a lead candidate for management...
Wed, 04/07/2021 - 08:02
by Emily Smith, Elizabeth Miller, Jeannette Munoz Aguayo, Cristian Flores Figueroa, Jill Nezworski, Marissa Studniski, Ben Wileman, Timothy Johnson Pasteurella multocida is a bacterial pathogen with the ability to infect a multitude of hosts including humans, companion animals, livestock, and wildlife. This study used bioinformatic approaches to explore the genomic diversity of 656 P. multocida...
Wed, 04/07/2021 - 08:02
by Clifford G. Clark, Ashley K. Kearney, Lorelee Tschetter, James Robertson, Frank Pollari, Stephen Parker, Gitanjali Arya, Kim Ziebell, Roger Johnson, John Nash, Celine Nadon Monophasic Salmonella 4,[5]:12:i:- are a major public health problem because they are one of the top five Salmonella serotypes isolated from clinical cases globally and because they can carry resistance to multiple...
Wed, 04/07/2021 - 08:02
by Goran Bozinovic, Damian Shea, Zuying Feng, David Hinton, Tim Sit, Marjorie F. Oleksiak Polycyclic aromatic hydrocarbons (PAHs) are among the most widespread natural and anthropogenic pollutants, and some PAHs are proven developmental toxicants. We chemically characterized clean and heavily polluted sites and exposed fish embryos to PAH polluted sediment extracts during four critical...
Wed, 04/07/2021 - 08:02
by Andrew Kanach, Theresa Bottorff, Min Zhao, Jun Wang, George T. C. Chiu, Bruce Applegate Due to the nascency of bacteriophage-based pathogen detection technologies, several practical hurdles stand in the way between providing promising proof-of-concept data and development of robust detection platforms. One such hurdle, and the focus of this work, is the development of methods for transitioning...
Sat, 03/27/2021 - 08:02
by Jun Wu, Ying Zhang, Zhongming Lv, Ping Yu, Weiqing Shi Aloe vera has been widely used in health and nutritional supplements in Chinese herbal medicine. Furthermore, Aloe vera production has been an emerging industry for making cosmetics and functional food. However, the reported adverse effects raised questions as to whether Aloe vera and its products were safe enough to be used in medicine...

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