PLoS One

Wed, 01/13/2021 - 08:01
by Wirittamulla Gamage Maheshika Kumudunie, Lakmini Inoka Wijesooriya, Yasanandana Supunsiri Wijayasinghe Rapidly progressing antibiotic resistance is a great challenge in therapy. In particular, the infections caused by carbapenem-resistant Enterobacteriaceae (CRE) are exceedingly difficult to treat. Carbapenemase production is the predominant mechanism of resistance in CRE. Early and accurate...
Tue, 01/12/2021 - 08:03
by Junenette L. Peters, Melissa J. Perry, Eileen McNeely, Robert O. Wright, Wendy Heiger-Bernays, Jennifer Weuve Elevated red blood cell distribution width (RDW), traditionally an indicator of anemia, has now been recognized as a risk marker for cardiovascular disease incidence and mortality. Experimental and acute exposure studies suggest that cadmium and lead individually affect red blood cell...
Tue, 01/12/2021 - 08:03
by Wei Sun, Yuanhua Wu, Dongxin Tang, Xiaoliang Li, Lei An Although several studies showed adverse neurotoxic effects of melamine on hippocampus (HPC)-dependent learning and reversal learning, the evidence for this mechanism is still unknown. We recently demonstrated that intra-hippocampal melamine injection affected the induction of long-term depression, which is associated with novelty...
Sat, 01/09/2021 - 08:01
by Omar Assafiri, Adelene Ai-Lian Song, Geok Hun Tan, Irwan Hanish, Amalia Mohd Hashim, Khatijah Yusoff Klebsiella pneumoniae are opportunistic bacteria found in the gut. In recent years they have been associated with nosocomial infections. The increased incidence of multiple drug-resistant K. pneumoniae makes it necessary to find new alternatives to treat the disease. In this study, phage...
Fri, 01/08/2021 - 08:01
by Hanae Miyagawa, Hiromichi Hara, Jun Araya, Shunsuke Minagawa, Takanori Numata, Yoshinori Umezawa, Akihiko Asahina, Hidemi Nakagawa, Kazuyoshi Kuwano Objectives Anti-IL-17/23 biologics are increasingly used to treat psoriasis. We aimed to elucidate characteristics of drug-induced interstitial pneumonia (DIIP) caused by anti-IL-17/23 biologics. Methods We retrospectively analyzed the clinical...
Fri, 01/08/2021 - 08:01
by Caroline Robé, Katrin Daehre, Roswitha Merle, Anika Friese, Sebastian Guenther, Uwe Roesler The colonization of broilers with extended-spectrum β-lactamase- (ESBL-) and plasmid-mediated AmpC β-lactamase- (pAmpC-) producing Enterobacteriaceae has been extensively studied. However, only limited data on intervention strategies to reduce the colonization throughout the fattening period are...
Wed, 01/06/2021 - 08:01
by Lena Diaz, Yong Li, Daniel M. Jenkins Constraints related to sample preparation are some of the primary obstacles to widespread deployment of molecular diagnostics for rapid detection of trace quantities (≤103 CFU/mL) of food-borne pathogens. In this research, we report a sample preparation method using a novel handheld electroflotation system to concentrate and recover dilute quantities (102−...
Fri, 01/01/2021 - 08:01
by Ji Yoon Choi, Da-An Huh, Kyong Whan Moon Imbalances in thyroid-stimulating hormone (TSH) levels are associated with metabolic syndrome (MetS), and the underlying mechanism is partly in alignment with that of lead exposure causing MetS. Many studies have reported the association between lead exposure and MetS, but no study has considered the possibility of TSH mediating lead's effect on MetS....
Fri, 01/01/2021 - 08:01
by Adam Wondmieneh, Getnet Gedefaw, Birhan Alemnew, Addisu Getie, Melaku Bimerew, Asmamaw Demis Background Intestinal parasitic infections are major public health problems throughout the world, particularly in people living with HIV/AIDS. People living with HIV/AIDS are vulnerable groups for a variety of diseases, hence they are easily affected by opportunistic and non-opportunistic intestinal...
Wed, 12/30/2020 - 08:01
by Paweł Staniszewski, Maciej Bilek, Wojciech Szwerc, Robert Tomusiak, Paweł Osiak, Ryszard Kocjan, Tadeusz Moskalik The aim of this study was to determine the effect of the age of trees, daily sap volume as well as the term of tapping birch sap collected in the forest environment on the content of selected minerals (zinc, copper and manganese) and heavy metals (lead, nickel, chromium and cadmium...
Thu, 12/24/2020 - 08:03
by Jianhui Zhen, Gang Liang, Ruichun Chen, Wenshen Jia Acetamiprid (ACE) is a kind of broad-spectrum pesticide that has potential health risk to human beings. Aptamers (Ap-DNA (1)) have a great potential as analytical tools for pesticide detection. In this work, a label-free electrochemical sensing assay for ACE determination is presented by electrochemical impedance spectroscopy (EIS). And the...
Thu, 12/24/2020 - 08:03
by Rafika Indah Paramita, Erni Juwita Nelwan, Fadilah Fadilah, Editha Renesteen, Nelly Puspandari, Linda Erlina Escherichia coli are one of the commonest bacteria causing bloodstream infection (BSI). The aim of the research was to identify the serotypes, MLST (Multi Locus Sequence Type), virulence genes, and antimicrobial resistance of E. coli isolated from bloodstream infection hospitalized...
Wed, 12/23/2020 - 08:01
by Diana Ibañez-Peinado, Maria Ubeda-Manzanaro, Antonio Martínez, Dolores Rodrigo The antimicrobial capability of chitosan from Tenebrio molitor as compared with chitosan from crustacean (Penaeus monodon) on different pathogenic microorganisms of concern in food safety was studied. The antimicrobial effect was tested at pH 5 and pH 6.2 and at two different initial concentrations (103 or 106 CFU/...
Tue, 12/22/2020 - 08:03
by Thorben Detering, Katharina Mundry, Ralf G. Berger Traditional smoke flavours bear the risk of containing a multitude of contaminating carcinogenic side-products. Enzymatic decarboxylation of ferulic acid released from agro-industrial side-streams by ferulic acid esterases (FAE) enables the sustainable generation of pure, food grade 4-vinylguaiacol (4-VG), the impact compound of smoke flavour...
Sat, 12/19/2020 - 08:01
by Sean Paz, Christopher Mauer, Anastasia Ritchie, Janet D. Robishaw, Massimo Caputi Widespread testing is required to limit the current public health crisis caused by the COVID-19 pandemic. Multiple tests protocols have been authorized by the food and drugs administration (FDA) under an emergency use authorization (EUA). The majority of these protocols are based on the gold-standard RT-qPCR test...
Sat, 12/12/2020 - 08:01
by Octavio Mesa-Varona, Heike Kaspar, Mirjam Grobbel, Bernd-Alois Tenhagen Antimicrobial resistance (AMR) is a global threat in humans and animals, and antimicrobial usage (AMU) has been identified as a main trigger of AMR. The purpose of this work was to compare data on AMR in clinical and non-clinical isolates of Escherichia coli in German broilers and turkeys between 2014 and 2017. Furthermore...
Sat, 12/12/2020 - 08:01
by Cong Li, Yu Liu, Jing Tian, Yanshu Zhu, Jinjuan Fan Sucrose metabolism contributes to the growth and development of plants and helps plants cope with abiotic stresses, including stress from Cd. Many of these processes are not well-defined, including the mechanism underlying the response of sucrose metabolism to Cd stress. In this study, we investigated how sucrose metabolism in maize varieties...
Fri, 12/11/2020 - 08:01
by Andreas Kern, Diane Shanahan, Roland Buesen, Dominik Geiger Mannanase 19287 enzyme is an engineered β-mannanase that can be added to diets for animals raised for human consumption to hydrolyze β-mannans. Established toxicological analyses were conducted with the enzyme preparation to ensure the safety of this product for the intended use. The mannanase 19287 preparation was produced with...
Fri, 12/11/2020 - 08:01
by Yonghong Mao, Yang Zhao, Yong Zhang, Hao Yang Native intact N-glycopeptide analysis can provide access to the comprehensive characteristics of N-glycan occupancy, including N-glycosites, N-glycan compositions, and N-glycoproteins for complex samples. The sample pre-processing method used for the analysis of intact N-glycopeptides usually depends on the enrichment of low abundance N-...
Thu, 12/10/2020 - 08:01
by Sonja Fiedler, Hannah Wünnemann, Isabel Hofmann, Natalie Theobalt, Annette Feuchtinger, Axel Walch, Julia Schwaiger, Rüdiger Wanke, Andreas Blutke Rainbow trout (Oncorhynchus mykiss) are frequently used as experimental animals in ecotoxicological studies, in which they are experimentally exposed to defined concentrations of test substances, such as heavy metals, pesticides, or pharmaceuticals...
Thu, 12/10/2020 - 08:01
by Peng Zhang, Qian Zhang, Hongwei Zhao, Yuanxin Li Aim This study aimed to explore factors may affect the length of hospital stay after laparoscopic appendectomy. Methods The data of 636 patients undergoing laparoscopic appendectomy between July 2016 and July 2019 in Beijing Tsinghua Changgung Hospital were retrospectively analyzed. The patients were divided into group A (hospital stay ≤3 days,...
Wed, 12/09/2020 - 08:01
by Ehtesham Iqbal, Risha Govind, Alvin Romero, Olubanke Dzahini, Matthew Broadbent, Robert Stewart, Tanya Smith, Chi-Hun Kim, Nomi Werbeloff, James H. MacCabe, Richard J. B. Dobson, Zina M. Ibrahim Objective Mining the data contained within Electronic Health Records (EHRs) can potentially generate a greater understanding of medication effects in the real world, complementing what we know from...
Tue, 12/08/2020 - 08:02
by Vicky L. M. N. Soomers, Winette T. A. van der Graaf, Shane Zaidi, Suzanne E. J. Kaal, Andrew J. Hayes, Bart H. W. B. Schreuder, Robin L. Jones, Ingrid M. E. Desar, Olga Husson Introduction Sarcomas are rare tumours. Early diagnosis is challenging, but important for local control and potentially survival and quality of life(QoL). We investigated (1)the route to diagnosis (RtD) experienced by...
Sat, 12/05/2020 - 08:02
by Sandip Patil, Amrita Rao, Preety Pathak, Swarali Kurle, Arati Mane, Amit Nirmalkar, A. K. Singhal, Vinita Verma, Mukesh Kumar Singh, D. C. S. Reddy, Ashwini Shete, Manjula Singh, Raman Gangakhedkar, Samiran Panda The integrated counseling and testing center (ICTC) located in the district hospital, Unnao in the northern state of Uttar Pradesh (UP), India witnessed an increased detection of HIV...
Fri, 12/04/2020 - 08:01
by Yitian Gao, Renjie Li, Wenqing Yang, Mei Zhou, Lei Wang, Chengbang Ma, Xinping Xi, Tianbao Chen, Chris Shaw, Di Wu Bradykinin-related peptides (BRPs) family is one of the most significant myotropic peptide families derived from frog skin secretions. Here, a novel BRP callitide was isolated and identified from the red-eyed leaf frog, Agalychnis callidryas, with atypical primary structure...
Tue, 12/01/2020 - 08:02
by Sutanu Nandi, Piyali Ganguli, Ram Rup Sarkar Essential gene prediction helps to find minimal genes indispensable for the survival of any organism. Machine learning (ML) algorithms have been useful for the prediction of gene essentiality. However, currently available ML pipelines perform poorly for organisms with limited experimental data. The objective is the development of a new ML pipeline...
Tue, 12/01/2020 - 08:02
by Biplob Kumar Modak, Partha Gorai, Devendra Kumar Pandey, Abhijit Dey, Tabarak Malik Introduction To explore the ethnobiological wisdom of the tribals of three western districts of West Bengal, India against poisonous and non-poisonous bites and stings, a quantitative approach was adopted. These age-old yet unexplored knowledge can be utilized in finding lead-molecules against poisonous and non...
Tue, 12/01/2020 - 08:02
by Yohannes Kebede, Zewdie Birhanu, Diriba Fufa, Yimenu Yitayih, Jemal Abafita, Ashenafi Belay, Abera Jote, Argaw Ambelu Background The endeavor to tackle the spread of COVID-19 effectively remains futile without the right grasp of perceptions and beliefs presiding in the community. Therefore, this study aimed to assess myths, beliefs, perceptions, and information gaps about COVID-19 in Ethiopia...
Wed, 11/25/2020 - 08:02
by Anna V. Skorynina, Emma G. Piligrimova, Olesya A. Kazantseva, Vladislav A. Kulyabin, Svetlana D. Baicher, Natalya A. Ryabova, Andrey M. Shadrin Several bacterial species belonging to the Bacillus cereus group are known to be causative agents of food poisoning and severe human diseases. Bacteriophages and their lytic enzymes called endolysins have been widely shown to provide for a supplemental...
Wed, 11/25/2020 - 08:02
by Mahmood Reza Ghorbani, Navid Ghanavati, Timoor Babaenejad, Ahad Nazarpour, Khoshnaz Payandeh The potential hazard to human health from exposure to heavy metals in surface soil was assessed using 66 soil samples collected from Ahvaz oil field. To this end, the contents of heavy metals were measured by the inductively coupled plasma spectroscopy (ICP-OES). Mean levels of As, Cd, Co, Cr, Cu, Ni,...
Wed, 11/25/2020 - 08:02
by José M. Di Diego, Bence Patocskai, Hector Barajas-Martinez, Virág Borbáth, Michael J. Ackerman, Alexander Burashnikov, Jérôme Clatot, Gui-Rong Li, Victoria M. Robinson, Dan Hu, Charles Antzelevitch Background J wave syndromes (JWS), including Brugada (BrS) and early repolarization syndromes (ERS), are associated with increased risk for life-threatening ventricular arrhythmias. Pharmacologic...
Tue, 11/24/2020 - 08:02
by David Yu, Karolina Ininbergs, Karolina Hedman, Christian G. Giske, Kristoffer Strålin, Volkan Özenci Purpose In the management of COVID-19, knowledge is lacking on the frequency of secondary bacterial infections and on how empirical antibiotic therapy should be used. In the present study, we aimed to compare blood culture (BC) results of a COVID-19 patient cohort with two cohorts of patients...
Tue, 11/24/2020 - 08:02
by Takako Kato-Minoura, Yutaro Ogiwara, Takashi Yamano, Hideya Fukuzawa, Ritsu Kamiya The single-cell green alga Chlamydomonas reinhardtii possesses two α-tubulin genes (tua1 and tua2) and two β-tubulin genes (tub1 and tub2), with the two genes in each pair encoding identical amino acid sequences. Here, we screened an insertional library to establish eight disruptants with defective tua2, tub1,...
Fri, 11/20/2020 - 08:01
by Fiona Sylvies, Lucy Nyirenda, Alden Blair, Kimberly Baltzell Background The misdiagnosis of non-malarial fever in sub-Saharan Africa has contributed to the significant burden of pediatric pneumonia and the inappropriate use of antibiotics in this region. This study aims to assess the impact of 1) portable pulse oximeters and 2) Integrated Management of Childhood Illness (IMCI) continued...
Fri, 11/20/2020 - 08:01
by Nishi H. Patel, Vanessa Meier-Stephenson, Meaza Genetu, Debasu Damtie, Ebba Abate, Shitaye Alemu, Yetework Aleka, Guido Van Marle, Kevin Fonseca, Carla S. Coffin, Tekalign Deressa Background Occult hepatitis B (OHB) is a major concern in HIV infected patients as it associates with a high risk of HBV reactivation and disease progression. However, data on the prevalence of OHB among HIV positive...
Fri, 11/20/2020 - 08:01
by Marta Gabryszewska, Barbara Gworek Polychlorinated biphenyls (PCBs) have been withdrawn from the market due to their toxicity, bioaccumulation capacity, and persistence. PCBs have been observed to potentially form in combustion processes under appropriate conditions and in the presence of precursors containing chlorine. The study covered a municipal waste incineration plant and an industrial...
Wed, 11/18/2020 - 08:02
by Shilpi Misra, Corina E. van Middelaar, Kieran Jordan, John Upton, Amy J. Quinn, Imke J. M. de Boer, Keelin O’Driscoll Pork is one of the most globally eaten meats and the pig production chain contributes significantly to the water footprint of livestock production. However, very little knowledge is available about the on-farm factors that influence freshwater use in the pig production chain....
Wed, 11/18/2020 - 08:02
by Akira Sakamaki, Kunihiko Yokoyama, Kyutaro Koyama, Shinichi Morita, Hiroyuki Abe, Kenya Kamimura, Masaaki Takamura, Shuji Terai In alcoholic liver cirrhosis (LC) patients, obesity has become a problem that progresses into liver dysfunction. Herein, we investigated the relationship between the prognosis of steatohepatitis and body weight, along with fat accumulation in patients with alcoholic...
Tue, 11/17/2020 - 08:02
by Olanrewaju J. Ogunrinu, Keri N. Norman, Javier Vinasco, Gizem Levent, Sara D. Lawhon, Virginia R. Fajt, Victoria V. Volkova, Tara Gaire, Toni L. Poole, Kenneth J. Genovese, Thomas E. Wittum, H. Morgan Scott Though carbapenems are not licensed for use in food animals in the U.S., carbapenem resistance among Enterobacteriaceae has been identified in farm animals and their environments. The...
Sat, 11/14/2020 - 08:02
by Victoria Brusa, Magdalena Costa, Nora L. Padola, Analía Etcheverría, Fernando Sampedro, Pablo S. Fernandez, Gerardo A. Leotta, Marcelo L. Signorini We developed a quantitative microbiological risk assessment (QMRA) of haemolytic uremic syndrome (HUS) associated with Shiga toxin-producing Escherichia coli (STEC)-contaminated beef (intact beef cuts, ground beef and commercial hamburgers) in...
Thu, 11/12/2020 - 08:02
by Casper C. Nyamukanza, Allan Sebata Broad- and fine-leaved woody species respond to seasonal changes from wet to dry season differently. For example, broad-leaved species shed their leaves earlier, while fine-leaved species, especially acacias retain green foliage well into the dry season. These differences are expected to result in variation in selection of broad- and fine-leaved woody species...
Thu, 11/05/2020 - 08:02
by Ngo Ngoc Quang Minh, Pham Van Toi, Le Minh Qui, Le Binh Bao Tinh, Nguyen Thi Ngoc, Le Thi Ngoc Kim, Nguyen Hanh Uyen, Vu Thi Ty Hang, Nguyen Thi Thuy Chinh B’Krong, Nguyen Thi Tham, Thai Dang Khoa, Huynh Duy Khuong, Pham Quynh Vi, Nguyen Ngoc Hong Phuc, Le Thi Minh Vien, Thomas Pouplin, Doan Van Khanh, Pham Nguyen Phuong, Phung Khanh Lam, Heiman F. L. Wertheim, James I. Campbell, Stephen Baker...
Wed, 11/04/2020 - 08:02
by Jessie Pinchoff, Carrie W. Mills, Deborah Balk Sub-Saharan Africa is experiencing rapid urban growth. Cities enable greater access to health services and improved water and sanitation infrastructure, leading to some improvements in health. However, urban settings may also be associated with more sedentary, stressful lifestyles and consumption of less nutritious food. C-reactive protein (CRP)...
Tue, 11/03/2020 - 08:02
by Siqi Liu, Luyao Wang, Chao Guo The core of urbanization is land use change, resulting in the urban sprawl and urban population explosion. The problem of land resources shortage and ecological environment destruction has become increasingly prominent. Land use change and human activities can directly lead to urban soil pollution. This study analyzed the concentration of Cd, Cr, Cu, Ni, Pb, Zn,...
Sat, 10/31/2020 - 08:02
by Ho-Wah Au, Man-Wah Tsang, Yu Wai Chen, Pui-Kin So, Kwok-Yin Wong, Yun-Chung Leung β-Lactam antibiotic detection has significant implications in food safety control, environmental monitoring and pharmacokinetics study. Here, we report the development of two BADAN-conjugated β-lactamases, E166Cb and E166Cb/N170Q, as sensitive biosensors for β-lactam antibiotic detection. These biosensors were...
Sat, 10/31/2020 - 08:02
by Reiko Abe, Hiroko Takagi, Kyoko Fujimoto, Katsuaki Sugiura The use of antimicrobial agents in food-producing animals may lead to the emergence and spread of antimicrobial resistance in bacteria of animal origin. The use of antimicrobial agents in pigs in 2018 in Japan was evaluated in terms of the weight of active ingredient and number of defined daily doses (DDD), using annual sales data of...
Sat, 10/31/2020 - 08:02
by Katinka Bekő, Zsuzsa Kreizinger, Cécile Yvon, Orsolya Saller, Salvatore Catania, Anneke Feberwee, Miklós Gyuranecz Mycoplasma synoviae infection occurs worldwide, leading to considerable economic losses in the chicken and turkey industry due to infectious synovitis, respiratory diseases and eggshell apex abnormalities. Control programs against M. synoviae infection are based on eradication,...
Wed, 10/28/2020 - 08:02
by Agnieszka Zimmermann, Agata Flis, Aleksandra Gaworska–Krzemińska, Marsha N. Cohen Introduction Nurses play a significant role in ensuring the safety and quality of drugs. Our aim was to assess significant factors in nurses’ participation in ensuring pharmacotherapy safety by reporting adverse drug reactions (ADR) and detecting substandard drugs (SD). Materials and methods The study was a cross...
Tue, 10/27/2020 - 08:02
by Deepti Pranay Samarth, Young Min Kwon Many epidemiological studies provide us with the evidence of horizontal gene transfer (HGT) contributing to the bacterial genomic diversity that benefits the bacterial populations with increased ability to adapt to the dynamic environments. Campylobacter jejuni, a major cause of acute enteritis in the U.S., often linked with severe post-infection...
Tue, 10/27/2020 - 08:02
by Mohammed Abdus Salam, Shujit Chandra Paul, Rabiatul Adawiyah M. Mohamad Zain, Snahasish Bhowmik, Mithun Rani Nath, Sadia Afrin Siddiqua, Tutun Das Aka, Muhammad Anwar Iqbal, Wan Rashidah Kadir, Rozita Binti Ahamad, Md. Abdul Khaleque, Aweng Eh Rak, Mohamad Faiz Mohd Amin The rapid growth of industrial and agricultural activities in Malaysia are leading to the impairment of most of the rivers...

Pages