PLoS Pathogens

Thu, 12/17/2020 - 08:03
by Stéphane Pont, Nathan Fraikin, Yvan Caspar, Laurence Van Melderen, Ina Attrée, François Cretin Bacterial bloodstream infections (BSI) are a major health concern and can cause up to 40% mortality. Pseudomonas aeruginosa BSI is often of nosocomial origin and is associated with a particularly poor prognosis. The mechanism of bacterial persistence in blood is still largely unknown. Here, we...
Wed, 12/09/2020 - 08:01
by Isabelle Leduc, Kristie L. Connolly, Afrin Begum, Knashka Underwood, Stephen Darnell, William M. Shafer, Jacqueline T. Balthazar, Andrew N. Macintyre, Gregory D. Sempowski, Joseph A. Duncan, Marguerite B. Little, Nazia Rahman, Eric C. Garges, Ann E. Jerse There is a pressing need for a gonorrhea vaccine due to the high disease burden associated with gonococcal infections globally and the rapid...
Wed, 12/09/2020 - 08:01
by Jamie L. Dombach, Joaquin L. J. Quintana, Toni A. Nagy, Chun Wan, Amy L. Crooks, Haijia Yu, Chih-Chia Su, Edward W. Yu, Jingshi Shen, Corrella S. Detweiler Infections caused by Gram-negative bacteria are difficult to fight because these pathogens exclude or expel many clinical antibiotics and host defense molecules. However, mammals have evolved a substantial immune arsenal that weakens...
Wed, 10/28/2020 - 08:02
by Mike Khan, Jerome S. Harms, Yiping Liu, Jens Eickhoff, Jin Wen Tan, Tony Hu, Fengwei Cai, Erika Guimaraes, Sergio Costa Oliveira, Richard Dahl, Yong Cheng, Delia Gutman, Glen N. Barber, Gary A. Splitter, Judith A. Smith Brucellosis, caused by a number of Brucella species, remains the most prevalent zoonotic disease worldwide. Brucella establish chronic infections within host macrophages...
Thu, 10/22/2020 - 08:02
by Mark Achtman, John Wain, François-Xavier Weill, Satheesh Nair, Zhemin Zhou, Vartul Sangal, Mary G. Krauland, James L. Hale, Heather Harbottle, Alexandra Uesbeck, Gordon Dougan, Lee H. Harrison, Sylvain Brisse, the S. enterica MLST study group
Tue, 10/20/2020 - 08:02
by Rujapak Sutiwisesak, Nathan D. Hicks, Shayla Boyce, Kenan C. Murphy, Kadamba Papavinasasundaram, Stephen M. Carpenter, Julie Boucau, Neelambari Joshi, Sylvie Le Gall, Sarah M. Fortune, Christopher M. Sassetti, Samuel M. Behar CD8 T cells provide limited protection against Mycobacterium tuberculosis (Mtb) infection in the mouse model. As Mtb causes chronic infection in mice and humans, we...
Sat, 10/17/2020 - 08:01
by Heba Barnawi, Nader Masri, Natasha Hussain, Bushra Al-Lawati, Evita Mayasari, Aleksandra Gulbicka, Adrian J. Jervis, Min-Hsuan Huang, Jennifer S. Cavet, Dennis Linton RNA thermometers (RNATs) trigger bacterial virulence factor expression in response to the temperature shift on entering a warm-blooded host. At lower temperatures these secondary structures sequester ribosome-binding sites (RBSs...
Tue, 10/13/2020 - 08:03
by Caroline Peron-Cane, José-Carlos Fernandez, Julien Leblanc, Laure Wingertsmann, Arnaud Gautier, Nicolas Desprat, Alice Lebreton Real-time imaging of bacterial virulence factor dynamics is hampered by the limited number of fluorescent tools suitable for tagging secreted effectors. Here, we demonstrated that the fluorogenic reporter FAST could be used to tag secreted proteins, and we implemented...
Tue, 10/13/2020 - 08:03
by Zhenyu Zhang, Haili Zhang, Ling Xu, Xing Guo, Wenfei Wang, Yujie Ji, Chaohui Lin, Yujie Wang, Xiaojun Wang The influenza B virus (IBV) causes seasonal influenza and has accounted for an increasing proportion of influenza outbreaks. IBV mainly causes human infections and has not been found to spread in poultry. The replication mechanism and the determinants of interspecies transmission of IBV...
Sat, 10/03/2020 - 08:02
by Li-hui He, Hao Wang, Yang Liu, Mei Kang, Tao Li, Chang-cheng Li, Ai-ping Tong, Yi-bo Zhu, Ying-jie Song, Stephen J. Savarino, Michael G. Prouty, Di Xia, Rui Bao Colonization factor CFA/I defines the major adhesive fimbriae of enterotoxigenic Escherichia coli and mediates bacterial attachment to host intestinal epithelial cells. The CFA/I fimbria consists of a tip-localized minor adhesive...
Tue, 09/29/2020 - 08:02
by Anubama Rajan, Matthew J. Robertson, Hannah E. Carter, Nina M. Poole, Justin R. Clark, Sabrina I. Green, Zachary K. Criss, Boyang Zhao, Umesh Karandikar, Yikun Xing, Mar Margalef-Català, Nikhil Jain, Reid L. Wilson, Fan Bai, Joseph M. Hyser, Joseph Petrosino, Noah F. Shroyer, Sarah E. Blutt, Cristian Coarfa, Xuezheng Song, BV Venkataram Prasad, Manuel R. Amieva, Jane Grande-Allen, Mary K....
Thu, 09/24/2020 - 08:03
by Vanessa Knittel, Pooja Sadana, Stephanie Seekircher, Anne-Sophie Stolle, Britta Körner, Marcel Volk, Cy M. Jeffries, Dmitri I. Svergun, Ann Kathrin Heroven, Andrea Scrima, Petra Dersch Type VI secretion systems (T6SSs) are complex macromolecular injection machines which are widespread in Gram-negative bacteria. They are involved in host-cell interactions and pathogenesis, required to eliminate...
Tue, 09/01/2020 - 08:03
by Erin A. Mack, Massimiliano S. Tagliamonte, Yu-Ping Xiao, Samantha Quesada, David R. Allred The tick-borne apicomplexan parasite, Babesia bovis, a highly persistent bovine pathogen, expresses VESA1 proteins on the infected erythrocyte surface to mediate cytoadhesion. The cytoadhesion ligand, VESA1, which protects the parasite from splenic passage, is itself protected from a host immune response...
Tue, 09/01/2020 - 08:03
by Yuen-Yan Chang, Virginie Stévenin, Magalie Duchateau, Quentin Giai Gianetto, Veronique Hourdel, Cristina Dias Rodrigues, Mariette Matondo, Norbert Reiling, Jost Enninga Shigella flexneri invades host cells by entering within a bacteria-containing vacuole (BCV). In order to establish its niche in the host cytosol, the bacterium ruptures its BCV. Contacts between S. flexneri BCV and infection-...
Thu, 08/27/2020 - 08:04
by Laura Belmont-Monroy, Waleska Saitz-Rojas, Jorge Soria-Bustos, Abigail S. Mickey, Nicholas E. Sherman, Benjamin C. Orsburn, Fernando Ruiz-Perez, Araceli E. Santiago Enteroaggregative Escherichia coli (EAEC) is a diarrheagenic pathotype associated with traveler’s diarrhea, foodborne outbreaks and sporadic diarrhea in industrialized and developing countries. Regulation of virulence in EAEC is...
Thu, 08/27/2020 - 08:04
by Junji Chida, Hideyuki Hara, Keiji Uchiyama, Etsuhisa Takahashi, Hironori Miyata, Hidetaka Kosako, Yukiko Tomioka, Toshihiro Ito, Hiroyuki Horiuchi, Haruo Matsuda, Hiroshi Kido, Suehiro Sakaguchi The cellular prion protein, PrPC, is a glycosylphosphatidylinositol anchored-membrane glycoprotein expressed most abundantly in neuronal and to a lesser extent in non-neuronal cells. Its conformational...
Wed, 08/26/2020 - 08:02
by Carmen Schwechheimer, Kassidy Hebert, Sarvind Tripathi, Praveen K. Singh, Kyle A. Floyd, Elise R. Brown, Monique E. Porcella, Jacqueline Osorio, Joseph T. M. Kiblen, Fernando A. Pagliai, Knut Drescher, Seth M. Rubin, Fitnat H. Yildiz Production of an extracellular matrix is essential for biofilm formation, as this matrix both secures and protects the cells it encases. Mechanisms underlying...
Mon, 08/24/2020 - 20:40
by Dannon Baker, Marius van den Beek, Daniel Blankenberg, Dave Bouvier, John Chilton, Nate Coraor, Frederik Coppens, Ignacio Eguinoa, Simon Gladman, Björn Grüning, Nicholas Keener, Delphine Larivière, Andrew Lonie, Sergei Kosakovsky Pond, Wolfgang Maier, Anton Nekrutenko, James Taylor, Steven Weaver The current state of much of the Wuhan pneumonia virus (severe acute respiratory syndrome...
Mon, 08/24/2020 - 20:40
by Jared D. Honeycutt, Nicolas Wenner, Yan Li, Susan M. Brewer, Liliana M. Massis, Sky W. Brubaker, Phoom Chairatana, Siân V. Owen, Rocío Canals, Jay C. D. Hinton, Denise M. Monack The various sub-species of Salmonella enterica cause a range of disease in human hosts. The human-adapted Salmonella enterica serovar Typhi enters the gastrointestinal tract and invades systemic sites to cause enteric...
Fri, 08/14/2020 - 08:01
by Rachel S. Resop, Rémi Fromentin, Daniel Newman, Hawley Rigsby, Larisa Dubrovsky, Michael Bukrinsky, Nicolas Chomont, Alberto Bosque Antiretroviral drugs that target various stages of the Human Immunodeficiency Virus (HIV) life cycle have been effective in curbing the AIDS epidemic. However, drug resistance, off-target effects of antiretroviral therapy (ART), and varying efficacy in prevention...
Wed, 08/12/2020 - 08:02
by Claire Scott, Jayakanth Kankanala, Toshana L. Foster, Daniel H. Goldhill, Peng Bao, Katie Simmons, Marieke Pingen, Matthew Bentham, Elizabeth Atkins, Eleni Loundras, Ruth Elderfield, Jolyon K. Claridge, Joseph Thompson, Peter R. Stilwell, Ranjitha Tathineni, Clive S. McKimmie, Paul Targett-Adams, Jason R. Schnell, Graham P. Cook, Stephen Evans, Wendy S. Barclay, Richard Foster, Stephen Griffin...
Sat, 07/25/2020 - 08:01
by Laetitia Canini, Barbara Holzer, Sophie Morgan, Johanneke Dinie Hemmink, Becky Clark, sLoLa Dynamics Consortium , Mark E. J. Woolhouse, Elma Tchilian, Bryan Charleston Influenza is a major cause of mortality and morbidity worldwide. Despite numerous studies of the pathogenesis of influenza in humans and animal models the dynamics of infection and transmission in individual hosts remain poorly...
Tue, 07/21/2020 - 08:01
by Elizabeth M. Adamowicz, Michaela Muza, Jeremy M. Chacón, William R. Harcombe With antibiotic resistance rates on the rise, it is critical to understand how microbial species interactions influence the evolution of resistance. In obligate mutualisms, the survival of any one species (regardless of its intrinsic resistance) is contingent on the resistance of its cross-feeding partners. This sets...
Sat, 07/18/2020 - 08:01
by Weihao Hong, Wenzhao Cheng, Tingjin Zheng, Nan Jiang, Ruian Xu Establishing latent infection but retaining the capability to reactivate in certain circumstance is an ingenious tactic for retroviruses to persist in vivo while evading host immune surveillance. Many evidences indicate that Human T-cell leukemia virus type 1 (HTLV-1) is not completely silent in vivo. However, signals that trigger...
Tue, 07/14/2020 - 08:01
by Alexander Kehl, Vera Göser, Tatjana Reuter, Viktoria Liss, Maximilian Franke, Christopher John, Christian P. Richter, Jörg Deiwick, Michael Hensel The intracellular lifestyle of Salmonella enterica is characterized by the formation of a replication-permissive membrane-bound niche, the Salmonella-containing vacuole (SCV). As a further consequence of the massive remodeling of the host cell...
Tue, 07/14/2020 - 08:01
by Jonathan S. Nguyen-Van-Tam, Ben Killingley, Joanne Enstone, Michael Hewitt, Jovan Pantelic, Michael L. Grantham, P. Jacob Bueno de Mesquita, Robert Lambkin-Williams, Anthony Gilbert, Alexander Mann, John Forni, Catherine J. Noakes, Min Z. Levine, LaShondra Berman, Stephen Lindstrom, Simon Cauchemez, Werner Bischoff, Raymond Tellier, Donald K. Milton, for the EMIT Consortium Uncertainty about...
Fri, 07/10/2020 - 08:01
by Amanda L. Miller, J. Alex Pasternak, Nicole J. Medeiros, Lauren K. Nicastro, Sarah A. Tursi, Elizabeth G. Hansen, Ryan Krochak, Akosiererem S. Sokaribo, Keith D. MacKenzie, Melissa B. Palmer, Dakoda J. Herman, Nikole L. Watson, Yi Zhang, Heather L. Wilson, R. Paul Wilson, Aaron P. White, Çagla Tükel Reactive arthritis, an autoimmune disorder, occurs following gastrointestinal infection with...
Wed, 07/08/2020 - 08:01
by Brittney N. Nguyen, Alfredo Chávez-Arroyo, Mandy I. Cheng, Maria Krasilnikov, Alexander Louie, Daniel A. Portnoy Listeria monocytogenes is a facultative intracellular bacterial pathogen that escapes from phagosomes and induces a robust adaptive immune response in mice, while mutants unable to escape phagosomes fail to induce a robust adaptive immune response and suppress the immunity to...
Tue, 07/07/2020 - 08:01
by Sarah K. Wilson, Justine Heckendorn, Bruno Martorelli Di Genova, Lindsey L. Koch, Peggy J. Rooney, Naomi Morrissette, Maryse Lebrun, Laura J. Knoll Toxoplasma gondii is an obligate intracellular parasite that can invade any nucleated cell of any warm-blooded animal. In a previous screen to identify virulence determinants, disruption of gene TgME49_305140 generated a T. gondii mutant that could...
Tue, 07/07/2020 - 08:01
by Minglu Gao, Anthony E. Glenn, Xi Gu, Trevor R. Mitchell, Timothy Satterlee, Mary V. Duke, Brian E. Scheffler, Scott E. Gold Sarocladium zeae is a fungal endophyte of maize and can be found co-inhabiting a single seed with Fusarium verticillioides, a major mycotoxigenic food safety threat. S. zeae produces pyrrocidines A and B that inhibit the growth of F. verticillioides and may limit its...
Fri, 07/03/2020 - 08:01
by Eli J. Cohen, Daisuke Nakane, Yoshiki Kabata, David R. Hendrixson, Takayuki Nishizaka, Morgan Beeby Campylobacter jejuni rotates a flagellum at each pole to swim through the viscous mucosa of its hosts’ gastrointestinal tracts. Despite their importance for host colonization, however, how C. jejuni coordinates rotation of these two opposing flagella is unclear. As well as their polar placement...
Fri, 07/03/2020 - 08:01
by Chihong Song, Reiko Takai-Todaka, Motohiro Miki, Kei Haga, Akira Fujimoto, Ryoka Ishiyama, Kazuki Oikawa, Masaru Yokoyama, Naoyuki Miyazaki, Kenji Iwasaki, Kosuke Murakami, Kazuhiko Katayama, Kazuyoshi Murata Norovirus is the major cause of epidemic nonbacterial gastroenteritis worldwide. Lack of structural information on infection and replication mechanisms hampers the development of...
Fri, 06/12/2020 - 08:01
by Savannah J. Taylor, Sebastian E. Winter Infection with nontyphoidal Salmonella strains, such as Salmonella enterica serovar Typhimurium (S. Typhimurium) commonly causes foodborne bacterial gastroenteritis. Studies on the pathogenesis of S. Typhimurium infection often focus on bona fide virulence factors. S. Typhimurium produces two distinct type III secretion systems that allow for the...
Wed, 05/27/2020 - 03:34
by Rafael S. Aquino, Atsuko Hayashida, Pyong Woo Park Heparan sulfate proteoglycans (HSPGs) are at the forefront of host-microbe interactions. Molecular and cell-based studies suggest that HSPG-pathogen interactions promote pathogenesis by facilitating microbial attachment and invasion of host cells. However, the specific identity of HSPGs, precise mechanisms by which HSPGs promote pathogenesis,...
Wed, 05/27/2020 - 03:34
by Roberta M. O’Connor, Felix J. Nepveux V, Jaypee Abenoja, Gregory Bowden, Patricia Reis, Josiah Beaushaw, Rachel M. Bone Relat, Iwona Driskell, Fernanda Gimenez, Michael W. Riggs, Deborah A. Schaefer, Eric W. Schmidt, Zhenjian Lin, Daniel L. Distel, Jon Clardy, Timothy R. Ramadhar, David R. Allred, Heather M. Fritz, Pradipsinh Rathod, Laura Chery, John White Apicomplexan parasites cause severe...
Tue, 05/12/2020 - 03:32
by Lamyaa Shaban, Giang T. Nguyen, Benjamin D. Mecsas-Faxon, Kenneth D. Swanson, Shumin Tan, Joan Mecsas Yersinia suppress neutrophil responses by using a type 3 secretion system (T3SS) to inject 6–7 Yersinia effector proteins (Yops) effectors into their cytoplasm. YopH is a tyrosine phosphatase that causes dephosphorylation of the adaptor protein SKAP2, among other targets in neutrophils. SKAP2...
Tue, 05/05/2020 - 03:34
by Qinghe Li, Fei Wang, Qiao Wang, Na Zhang, Jumei Zheng, Maiqing Zheng, Ranran Liu, Huanxian Cui, Jie Wen, Guiping Zhao As a canonical adaptor for the Toll-like receptor (TLR) family, myeloid differentiation primary response protein 88 (MyD88) has crucial roles in host defense against infection by microbial pathogens, and its dysregulation might induce autoimmune diseases. Here, we demonstrate...
Tue, 05/05/2020 - 03:34
by Stefan A. Fattinger, Desirée Böck, Maria Letizia Di Martino, Sabrina Deuring, Pilar Samperio Ventayol, Viktor Ek, Markus Furter, Saskia Kreibich, Francesco Bosia, Anna A. Müller-Hauser, Bidong D. Nguyen, Manfred Rohde, Martin Pilhofer, Wolf-Dietrich Hardt, Mikael E. Sellin Salmonella enterica serovar Typhimurium (S.Tm) infections of cultured cell lines have given rise to the ruffle model for...
Fri, 04/17/2020 - 03:24
by Julia María Coronas-Serna, Arthur Louche, María Rodríguez-Escudero, Morgane Roussin, Paul R. C. Imbert, Isabel Rodríguez-Escudero, Laurent Terradot, María Molina, Jean-Pierre Gorvel, Víctor J. Cid, Suzana P. Salcedo Brucella species are facultative intracellular Gram-negative bacteria relevant to animal and human health. Their ability to establish an intracellular niche and subvert host cell...
Thu, 04/16/2020 - 03:25
by Muhammad Fahim Raza, Yichen Wang, Zhaohui Cai, Shuai Bai, Zhichao Yao, Umar Anwar Awan, Zhenyu Zhang, Weiwei Zheng, Hongyu Zhang Gut symbiotic bacteria have a substantial impact on host physiology and ecology. However, the contribution of gut microbes to host fitness during long-term low-temperature stress is still unclear. This study examined the role of gut microbiota in host low-temperature...
Thu, 04/16/2020 - 03:25
by Leo Yi Yang Lee, Jie Zhou, Rebecca Frise, Daniel H. Goldhill, Paulina Koszalka, Edin J. Mifsud, Kaoru Baba, Takahiro Noda, Yoshinori Ando, Kenji Sato, Aoe-Ishikawa Yuki, Takao Shishido, Takeki Uehara, Steffen Wildum, Elke Zwanziger, Neil Collinson, Klaus Kuhlbusch, Barry Clinch, Aeron C. Hurt, Wendy S. Barclay Influenza viruses cause seasonal outbreaks and pose a continuous pandemic threat....
Tue, 04/14/2020 - 03:25
by Camille Rey, Yuen-Yan Chang, Patricia Latour-Lambert, Hugo Varet, Caroline Proux, Rachel Legendre, Jean-Yves Coppée, Jost Enninga Microfold (M) cell host-pathogen interaction studies would benefit from the visual analysis of dynamic cellular and microbial interplays. We adapted a human in vitro M cell model to physiological bacterial infections, expression of fluorescent localization reporters...
Tue, 04/14/2020 - 03:25
by Shauna M. Crowley, Xiao Han, Joannie M. Allaire, Martin Stahl, Isabella Rauch, Leigh A. Knodler, Bruce A. Vallance We investigated the role of the inflammasome effector caspases-1 and -11 during Salmonella enterica serovar Typhimurium infection of murine intestinal epithelial cells (IECs). Salmonella burdens were significantly greater in the intestines of caspase-1/11 deficient (Casp1/11−/−),...
Tue, 04/07/2020 - 03:42
by Vincent R. Graziano, Forrest C. Walker, Elizabeth A. Kennedy, Jin Wei, Khalil Ettayebi, Madison S. Strine, Renata B. Filler, Ebrahim Hassan, Leon L. Hsieh, Arthur S. Kim, Abimbola O. Kolawole, Christiane E. Wobus, Lisa C. Lindesmith, Ralph S. Baric, Mary K. Estes, Robert C. Orchard, Megan T. Baldridge, Craig B. Wilen Murine norovirus (MNoV) is an important model of human norovirus (HNoV) and...
Fri, 04/03/2020 - 03:23
by Raquel Tarancón, Jorge Domínguez-Andrés, Santiago Uranga, Anaísa V. Ferreira, Laszlo A. Groh, Mirian Domenech, Fernando González-Camacho, Niels P. Riksen, Nacho Aguilo, José Yuste, Carlos Martín, Mihai G. Netea Among infectious diseases, tuberculosis is the leading cause of death worldwide, and represents a serious threat, especially in developing countries. The protective effects of Bacillus...
Fri, 03/13/2020 - 03:23
by Yuxin Zhou, X. Edward Zhou, Yuanping Gong, Yuanye Zhu, Xiaoman Cao, Joseph S. Brunzelle, H. Eric Xu, Mingguo Zhou, Karsten Melcher, Feng Zhang Fusarium is a genus of filamentous fungi that includes species that cause devastating diseases in major staple crops, such as wheat, maize, rice, and barley, resulting in severe yield losses and mycotoxin contamination of infected grains. Phenamacril is...
Tue, 03/10/2020 - 03:23
by Saori Oku, Toru Takeshita, Toshiko Futatsuki, Shinya Kageyama, Mikari Asakawa, Yasuo Mori, Toshihiro Miyamoto, Jun Hata, Toshiharu Ninomiya, Haruhiko Kashiwazaki, Yoshihisa Yamashita Disruption of the intestinal microbiota caused by intensive chemotherapy, irradiation and antibiotics can result in development of severe gut graft-versus-host disease and infectious complications, leading to...
Tue, 02/25/2020 - 03:27
by Michele Lunelli, Antje Kamprad, Jörg Bürger, Thorsten Mielke, Christian M. T. Spahn, Michael Kolbe The Type III Secretion Systems (T3SS) needle complex is a conserved syringe-shaped protein translocation nanomachine with a mass of about 3.5 MDa essential for the survival and virulence of many Gram-negative bacterial pathogens. This system is composed of a membrane-embedded basal body and an...
Tue, 02/25/2020 - 03:27
by Lenka Cernikova, Carmen Faso, Adrian B. Hehl Phosphorylated derivatives of phosphatidylinositol (PIPs), are key membrane lipid residues involved in clathrin-mediated endocytosis (CME). CME relies on PI(4,5)P2 to mark endocytic sites at the plasma membrane (PM) associated to clathrin-coated vesicle (CCV) formation. The highly diverged parasitic protist Giardia lamblia presents disordered and...
Sat, 02/22/2020 - 03:26
by Haili Zhang, Hongxin Li, Wenqiang Wang, Yujie Wang, Guan-Zhu Han, Hualan Chen, Xiaojun Wang Both the replication and transcription of the influenza virus are catalyzed by the viral polymerase complex. The polymerases of most avian influenza A viruses have poor performance in mammalian cells, which is considered to be one of the important species barriers. Pigs have been long considered as...

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