Veterinary Microbiology

Mon, 01/11/2021 - 06:05
Author(s): Jing Zhuang, Xiaoyue Ji, Yue Zhu, Wei Liu, Jing Sun, Xinan Jiao, Xiulong Xu Autophagy plays an important role in restricting the growth of invading intracellular microbes. Salmonella (S) Typhimurium, an intracellular pathogen that causes gastroenteritis and food poisoning in humans, evades autophagic detection by multiple mechanisms. There has been growing interest in developing...
Thu, 11/26/2020 - 06:15
Author(s): Yizhi Tang, Yan Lai, Xingyuan Wang, Changwei Lei, Chao Li, Linghan Kong, Yulong Wang, Hongning Wang The optrA gene encodes an ABC-F protein which confers cross-resistance to oxazolidinones and phenicols, and its transmission has so far been associated with multiple transposable elements, including IS1216E, prophages, ICEs and Tn558. Here, we identified an optrA gene flanked by two...
Thu, 11/26/2020 - 06:15
Author(s): Jiyun Li, Yuqing Zhou, Dawei Yang, Shan Zhang, Zhiliang Sun, Yang Wang, Shaolin Wang, Congming Wu The prevalence and antimicrobial susceptibility of Clostridium perfringens (C. perfringens) in chickens and pigs were investigated in Beijing and Shanxi, China. In total, 322 C. perfringens (chicken n = 60 and pig n = 262) were obtained from 620 feces of chickens (n = 256) and pigs (n = ...
Wed, 11/11/2020 - 06:09
Shiga toxin–producing E. coli (STEC) and enterotoxigenic E. coli (ETEC) are the main agents of swine colibacillosis, an infectious disease which implies important economic losses. We characterized here 186 diarrheagenic E. coli from Spanish industrial pig farms (2005-2017) to know which clones were involved in this syndrome, and the rates of antibiotic resistance. The PCR based on pathotype-...
Thu, 10/01/2020 - 06:15
Author(s): Maria Isabel Nogueira Di Azevedo, Bruno C. Pires, Hugo Libonati, Priscila S. Pinto, Lucas Figueiredo Cardoso Barbosa, Filipe Anibal Carvalho-Costa, Walter Lilenbaum Bovine genital leptospirosis is a chronic disease that causes reproductive disorders such as abortions, stillbirths, and estrus repetition, as well as economic losses. Despite clinical signs related to reproductive failure...
Sun, 09/27/2020 - 06:11
Author(s): Honghong Xian, Yu Yuan, Chao Yin, Zhenyu Wang, Ruoyun Ji, Chao Chu, Xinan Jiao, Qiuchun Li Salmonella Pathogenicity Island 19 (SPI-19) encoded type VI secretion system (T6SS) is a virulence factor present in few serotypes of S. enterica, including S. Dublin, S. Gallinarum and S. Pullorum. Comparative genomic sequence analysis revealed that the gene clusters of SPI-19 showed high...
Thu, 09/10/2020 - 06:14
Author(s): Yinjie Niu, Baihan Liu, Chang Sun, Lili Zhao, Hongyan Chen Duck hepatitis A virus type 1 (DHAV-1) disease causes significant economic losses to the duck industry. Duck enteritis virus (DEV) is frequently used as a viral vector for aquatic poultry vaccination, but no recombinan DEV expressing DHAV-1 VP0 has been developed. In this study, we established a system for rescuing the DEV C-...
Mon, 08/24/2020 - 17:53
Author(s): Shudan Liu, Puxian Fang, Wenting Ke, Jing Wang, Xunlei Wang, Shaobo Xiao, Liurong Fang Porcine deltacoronavirus (PDCoV) is a novel swine enteropathogenic coronavirus that causes watery diarrhea, vomiting and mortality in nursing piglets. Type III interferons (IFN-λs) are the major antiviral cytokines in intestinal epithelial cells, the target cells in vivo for PDCoV. In this study, we...
Mon, 08/24/2020 - 17:53
Author(s): Bai Wei, Ke Shang, Se-Yeoun Cha, Jun-Feng Zhang, Min Kang, Hyung-Kwan Jang Salmonella is a type of zoonotic bacteria that represents an economic and public health concern worldwide. Difficulties in sample collection from migratory birds mean little is known about their importance as a reservoir of Salmonella. The present study evaluated the prevalence and potential risk of Salmonella...
Fri, 08/07/2020 - 06:09
Author(s): Suyun Liang, Ming Xie, Jing Tang, Minghang Wang, Dabing Zhang, Shuisheng Hou Duck hepatitis A virus genotypes 3 (DHAV-3) has become the most prevalent pathogen of duck viral hepatitis (DVH) in Asian duck industry in recent years. Previous studies on the pathogenic mechanism of DHAV-3 mainly focused on examine host gene expression levels. However, the study about host protein expression...
Fri, 07/17/2020 - 06:07
Author(s): Luis Figueras, Luis Miguel Ferrer, José María González, Juan Pedro Bueso, Juan José Ramos, Inés Rubira, Erika Burian, Delia Lacasta Salmonella enterica subsp. diarizonae serotype 61: k: 1,5, (7) (SED) is a microorganism well adapted to sheep; however, it has also been described producing chronic proliferative rhinitis (CPR) in ovine. CPR causes a proliferative inflammation of the...
Tue, 07/14/2020 - 06:09
Author(s): Suvi Joutsen, Per Johansson, Riikka Laukkanen-Ninios, Johanna Björkroth, Maria Fredriksson-Ahomaa Yersinia enterocolitica is the most common Yersinia species causing foodborne infections in humans. Pathogenic strains carry the chromosomal ail gene, which is essential for bacterial attachment to and invasion into host cells and for serum resistance. This gene is commonly amplified in...
Wed, 06/24/2020 - 06:07
Author(s): Kaituo Liu, Ruyi Gao, Xiaoquan Wang, Wenwen Han, Zhuxing Ji, Huafen Zheng, Min Gu, Jiao Hu, Xiaowen Liu, Shunlin Hu, Sujuan Chen, Song Gao, Daxin Peng, Xin-an Jiao, Xiufan Liu Pigeons were previously thought to be resistant to H5 viruses and to play a minimal role in spreading these viruses. In this study, we evaluated the pathogenicity of two clade 2.3.4.4 H5N6 viruses in pigeons and...
Tue, 06/23/2020 - 06:09
Author(s): Julia Sabio y García, María M. Bigi, Laura I. Klepp, Federico C. Blanco, Fabiana Bigi Members of the Mycobacterium tuberculosis complex (MTBC) are responsible for tuberculosis in several mammals. In this complex, Mycobacterium tuberculosis and Mycobacterium bovis, which are closely related, show host preference for humans and cattle, respectively. Although human and bovine tuberculosis...
Mon, 06/22/2020 - 06:07
Author(s): Apostolos S. Angelidis, Dimitrios Komodromos, Rebecca Giannakou, Georgios Arsenos, Athanasios I. Gelasakis, Maria Kyritsi, George Filioussis, Christos Hadjichristodoulou, Pavlina Torounidou, Anna Papa, Daniel Sergelidis The presence of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in raw milk is a challenge for veterinarians and public health professionals. In this...
Fri, 06/12/2020 - 06:06
Author(s): Mingjun Sun, Mengda Liu, Xiyue Zhang, Ge Zhang, Liangquan Zhu, Jiabo Ding, Zhicheng Zhang, Shixiong Sun, Shufang Sun, Weixing Shao, Peipei Zhang, Jiandong Zhang, Xiangxiang Sun, Xuezheng Fan, Rong Wei Brucellosis is one of the major zoonotic diseases in the world. In China, understanding on its causative agent Brucella is still limited. Recently, we isolated a Brucella strain XZ19-1...
Thu, 06/04/2020 - 03:33
Author(s): Zuxian Chen, Zhenyu Wang, Xiya Zhao, Yun Guan, Qian Xue, Jinrong Li, Zhiting Liu, Bingbing Zhao, Zhuoliang He, Jianni Huang, Ming Liao, Yafen Song, Peirong Jiao The H5N6 highly pathogenic avian influenza virus (HPAIV) has been circulating in China since 2013. In this report, we describe our recent chicken experimental studies investigating the pathogenicity and transmission of four...
Thu, 06/04/2020 - 03:33
Author(s): Rui-hua Zhang, Pei-yao Li, Ming-Ju Xu, Cun-Lian Wang, Chun-Hong Li, Jing-ping Gao, Xue-jing Wang, Tong Xu, Hong-liang Zhang, Rui-hong Zhang, Shu-Fei Tian H9N2 avian influenza viruses (AIVs) can cross species barriers and expand from birds tomammals and humans. It usually leads to economic loss for breeding farms and poses a serious threat to human health.This study investigated the...
Thu, 05/21/2020 - 03:34
Author(s): Bao-Yang Ruan, Yun Yao, Shuai-Yong Wang, Xiao-Qian Gong, Xiao-Min Liu, Qi Wang, Ling-Xue Yu, Shi-Qiang Zhu, Juan Wang, Tong-Ling Shan, Yan-Jun Zhou, Wu Tong, Hao Zheng, Guo-Xin Li, Fei Gao, Ning Kong, Hai Yu, Guang-Zhi Tong The classical swine (CS) H1N1 swine influenza virus (SIVs) emerged in humans as a reassortant virus that caused the H1N1 influenza virus pandemic in 2009, and the...
Thu, 05/21/2020 - 03:34
Author(s): Weiwei Wang, Jingzhen Liang, Mengya Shi, Guo Chen, Yu Huang, Yan Zhang, Zengzhi Zhao, Min Wang, Min Li, Meilan Mo, Tianchao Wei, Teng Huang, Xiumiao He, Ping Wei Duck spleen necrosis disease (DSND) is an emerging infectious disease that causes significant economic loss in the duck industry. In 2018, a duck reovirus (named DRV/GX-Y7) and Salmonella indiana were both isolated from the...
Mon, 05/18/2020 - 03:31
Author(s): Duc Kieu Minh, Yaovi M. Gildas Hounmanou, Huong Bui Thi Mai, John E. Olsen, Anders Dalsgaard Vietnam is the world’s fifth largest producer of pork meat. Salmonella is frequently found at farm level; however, risk factors for Salmonella infection in pigs have not been thoroughly investigated in the production system. In the current study, 123 commercial feed samples were obtained from...
Fri, 05/01/2020 - 03:30
Author(s): Lucía P. Alvarez, Nazaret Ruiz-Villalobos, Marcela Suárez-Esquivel, Nicholas R. Thomson, Romanela Marcellino, Eunice Víquez-Ruiz, Carlos A. Robles, Caterina Guzmán-Verri Brucellosis in rams is caused by Brucella ovis or Brucella melitensis and it is considered one of the most important infectious diseases of males in sheep-raising countries. Molecular characterization of Brucella spp....
Fri, 03/27/2020 - 03:36
Author(s): Mingxing Tian, Yi Yin, Zhengmin Lian, Zichen Li, Meiying Song, Hai Hu, Xiang Guan, Chan Ding, Shaohui Wang, Tao Li, Jingjing Qi, Shengqing Yu Brucella is a facultative intracellular bacterium, dividing into smooth- and rough-type Brucella. Smooth-type Brucella can dissociate into rough mutants with cytotoxicity for macrophages during infection, which is critical for Brucella egress and...
Sun, 03/22/2020 - 03:20
Author(s): Min-Che Tung, Hsin-Yu Lu, Yu-Kang Chang, Wei-Ru Huang, Tsai-Ling Liao, Hung-Yi Wu, Ching-Dong Chang, Hueng-Chuen Fan, Brent L. Nielsen, Hung-Jen Liu In the present study, we have generated several H5N2 HA recombinant baculoviruses for production of a HA subunit vaccine against the lethal H5N2 avian influenza virus (AIV). The effective display of functional HA on the cell membrane and...
Wed, 03/04/2020 - 03:37
Author(s): Amal Senevirathne, Chamith Hewawaduge, John Hwa Lee Salmonella Enteritidis (SE) is one of the most common culprits of foodborne disease in humans due to its horizontal transmission from infected animals to humans. The development of a safe vaccine against Salmonella would be important for both farm animals and humans concerning disease containment. The SE ghosts carrying FliC were...
Thu, 02/20/2020 - 03:23
Author(s): Haejin Hwang, Richard E. Isaacson, Randall S. SingerAbstractCampylobacteriosis caused by C. jejuni is a serious yet common foodborne disease in the U.S. The prevalence of fluoroquinolone-resistant C. jejuni from poultry has continued to increase despite the withdrawal of fluoroquinolone use in the U.S. poultry industry in 2005. To date, no clear selective pressures that explain this...
Sat, 02/15/2020 - 03:22
Author(s): Sankar Renu, Ninoshkaly Feliciano-Ruiz, Shristi Ghimire, Yi Han, Jennifer Schrock, Santosh Dhakal, Veerupaxagouda Patil, Steven Krakowka, Gourapura J. RenukaradhyaAbstractTo improve the innate and adaptive immune responses elicited by an inactivated swine influenza virus antigen (KAg)-loaded chitosan nanoparticles (CS NPs-KAg), we used the adjuvant, poly(I:C). The formulated CS NPs-KAg...
Wed, 02/12/2020 - 10:46
Author(s): Natalia Mazur-Panasiuk, Grzegorz WoźniakowskiAbstractAfrican swine fever virus can be transmitted through direct contact with infected animals and their excretions, or indirect contact with contaminated fomites. Risk assessment of the disease spreading requires quantitative knowledge about time and conditions needed for its inactivation in various material of pig origin. In this study...
Mon, 02/10/2020 - 03:38
Author(s): Priyangi A Alwis, Puthayalai Treerat, Lan Gong, Deanna Deveson Lucas, Elizabeth M Allwood, Mark Prescott, Rodney J Devenish, Ben Adler, John D BoyceAbstractTwo-component signal transduction systems (TCSTS) are abundant among prokaryotes and regulate important functions, including drug resistance and virulence. The Gram-negative bacterium Burkholderia pseudomallei, which causes the...
Mon, 01/27/2020 - 03:22
Author(s): Mar Costa-Hurtado, Emili Barba-Vidal, Jaime Maldonado, Virginia AragonAbstractAntimicrobials have been commonly used to control bacterial diseases in farm animals. The efficacy of these drugs deterred the development of other control measures, such as vaccines, which are currently getting more attention due to the increased concern about antimicrobial resistance. Glässer’s disease is...
Sun, 01/26/2020 - 03:21
Author(s): Johannes Hamel, Yanchao Zhang, Nicole Wente, Volker KrömkerAbstractNon-S. aureus staphylococci (NAS) are the most frequently isolated pathogens from bovine milk and can cause intramammary infections (IMI). They can also be found in teat canals, on bovine skin and in cows’ environment, which may lead to unnoticed contamination of milk samples. The aim of this study was to investigate...
Sun, 01/26/2020 - 03:21
Author(s): Elisabetta Razzuoli, Walter Vencia, Paola Modesto, Giulia Franzoni, Silvia Dei Giudici, Erica Parisi, Angelo Ferrari, Massimo AmadoriAbstractGut is often subject to infection by different pathogens like Y. enterocolitica. To date, biotypes (BTs) 1A have been considered as non-pathogenic, because they do not express plasmid of virulence pYV; however, BTs 1A strains present other...
Tue, 01/14/2020 - 03:20
Author(s): Alisha Wehdnesday Bernardo Reyes, Son Hai Vu, Tran Xuan Ngoc Huy, WonGi Min, Hu Jang Lee, Hong Hee Chang, John Hwa Lee, Suk KimAbstractBrucella as a stealthy intracellular pathogen avoids activation of innate immune response. Here we investigated the contribution of an adenosine receptor, Adora2b, during Brucella infection in professional phagocyte RAW 264.7 cells and in a murine model...
Wed, 01/08/2020 - 03:23
Author(s): João Anes, Scott V. Nguyen, Athmanya K. Eshwar, Evonne McCabe, Guerrino Macori, Daniel Hurley, Angelika Lehner, Séamus FanningAbstractAntimicrobial resistance reported in bacteria of animal origin is considered a major challenge to veterinary public health. In this study, the genotypic and phenotypic characterisation of twelve Escherichia coli isolates of bovine origin is reported....
Sat, 01/04/2020 - 03:22
Author(s): Qi Su, Zhihui Zhang, Yawen Zhang, Zhizhong Cui, Shuang Chang, Peng ZhaoAbstractSince 2016, severe outbreaks of hepatic rupture hemorrhage syndrome (HRHS) associated with infections of tentative novel avian hepatitis E virus (HEV) have emerged in chickens in China, causing increased mortality and decreased laying rate in adult hens and disturbing the hatching and breeding of chicks. To...
Thu, 01/02/2020 - 03:22
Author(s): Dieudonné Tialla, Aurélie Sausy, Assana Cissé, Tani Sagna, Abdoul Kader Ilboudo, Georges Anicet Ouédraogo, Judith M. Hübschen, Zékiba Tarnagda, Chantal J. SnoeckAbstractDespite improvement of human and avian influenza surveillance, swine influenza surveillance in sub-Saharan Africa is scarce and pandemic preparedness is still deemed inadequate, including in Burkina Faso. This cross-...
Mon, 12/30/2019 - 03:22
Author(s): Sonsiray Álvarez-Narváez, Catherine M. Logue, Nicolle Lima Barbieri, Londa J. Berghaus, Steeve GiguèreAbstractBackgroundRhodococcus equi (R. equi) infections are endemic in many horse facilities in the United States resulting significant economic loses annually. Currently, there is no commercial vaccine available and the emergence of isolates that are resistant to the current treatment...
Sun, 12/29/2019 - 03:23
Author(s): Wenjuan He, Shizhen Ma, Lei Lei, Junjia He, Xing Li, Jin Tao, Xueyang Wang, Shikai Song, Yongqiang Wang, Yang Wang, Jianzhong Shen, Chang Cai, Congming WuAbstractThis study investigated the continuous monthly prevalence of bovine clinical mastitis (CM) and the distribution of causative pathogens among 36,619 CM milk samples from large dairy farms across seven Chinese provinces from...
Sat, 12/28/2019 - 03:21
Author(s): Bisvanath Patra, Manjit Panigrahi, Sheikh Firdous Ahmad, Satyabrata Dandapat, Pushpendra Kumar, Bharat BhushanAbstractIn the present study, we analyzed the immune response of calves to Brucella abortus strain 19 vaccine (S19) and its association with MHC class I (BoLA-A) alleles (exons 2-3 and 4-5). Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used...
Tue, 12/17/2019 - 03:22
Author(s): Patrizia Casagrande Proietti, Gabriella Guelfi, Sara Bellucci, Silvio De Luca, Settimio Di Gregorio, Camillo Pieramati, Maria Pia FranciosiniAbstractThe objectives of this work were to evaluate β-lactamase-mediated β-lactam resistance in Campylobacter coli and Campylobacter jejuni isolates obtained from broiler chickens, expression of the blaOXA-61 gene in relation to β-lactamase...
Thu, 12/05/2019 - 03:40
Author(s): Hang Yin, Zhijie Li, Jikai Zhang, Jiapei Huang, Hongtao Kang, Jin Tian, Liandong QuAbstractSince 2011, to control the spread of pseudorabies (PR), US7/US8/UL23-deleted recombinant PRV (rPRV) vaccines based on current variants have been developed. The vaccines can provide effective immune protection to pigs, but fur-bearing animals, such as dogs, foxes, and minks, are increasingly...
Mon, 12/02/2019 - 03:22
Author(s): Chang-Wei Lei, Yu Zhang, Zhuang-Zhuang Kang, Ling-Han Kong, Yi-Zhi Tang, An-Yun Zhang, Xin Yang, Hong-Ning WangAbstractHuman salmonellosis caused by the consumption of eggs and chicken meat contaminated with Salmonella Enteritidis has become a continuing public health concern worldwide. In this study we adopted whole genome sequencing (WGS) to determine the genetic relationship and...
Mon, 12/02/2019 - 03:22
Author(s): Mayada Gwida, Amal Awad, Maged El-Ashker, Helmut Hotzel, Stefan Monecke, Ralf Ehricht, Elke Müller, Annett Reißig, Stefanie A. Barth, Christian Berens, Sascha D. BraunAbstractThe objective of our study was to provide a molecular analysis using DNA-microarray based assays of commensal E. coli populations from apparently healthy livestock and their attendants to assess the virulence...
Mon, 12/02/2019 - 03:22
Author(s): Maged El-Ashker, Mayada Gwida, Stefan Monecke, Fatma El-Gohary, Ralf Ehricht, Mona Elsayed, Akinduti Paul, Mohamed El-Fateh, Sven MaurischatAbstractMethicillin-resistant S. aureus from cows with mastitis has received a growing interest worldwide. The present study aimed to provide a detailed description of the resistance and virulence traits of isolates from bovine mastitis samples.A...
Mon, 12/02/2019 - 03:22
Author(s): Doris Aper, Janna Frömbling, Murat Bağcıoğlu, Monika Ehling-Schulz, Isabel Hennig-PaukaAbstractMost outbreaks of disease due to infection with Actinobacillus (A.) pleuropneumoniae are caused by pigs already pre-colonised in tonsillar tissue, where the pathogen is protected from exposure to antibiotic substances administered for treatment. As it has been shown recently under...
Fri, 11/29/2019 - 03:17
Author(s): SeEun Choe, Sok Song, Gyu-Nam Park, Jihye Shin, Ki-Sun Kim, Ra MiCha, Bang-Hun Hyun, Bong-Kyun Park, Dong-Jun AnABSTRACTThe hepatitis E virus (HEV) is known to have 4 genotypes but only one serotype. Genotype 1 and 2 infect humans only and genotype 3 and 4 infect humans, pigs and other animal species. Pig and wild boar are also known as reservoirs of HEV infection. Of the 2,736 wild...
Thu, 11/28/2019 - 03:37
Author(s): Clarissa Silveira Luiz Vaz, Daiane Voss-Rech, Luana Alves, Arlei Coldebella, Liana Brentano, Iara Maria TrevisolAbstractSalmonella Enteritidis remains a leading cause of human foodborne disease, mostly associated with the consumption of contaminated poultry products. To more strategically implement a phage therapy scheme for S. Enteritidis control in broilers, a cocktail containing...
Thu, 11/28/2019 - 03:37
Author(s): Antonise M. Jaguezeski, Aleksandro S. da Silva, Teane M.A. Gomes, Nathieli B. Bottari, Thalisson F. Lopes, Renan A. Cechin, Vera M. Morsch, Maria R.C. Schetinger, Janice L. Giongo, Rodrigo de A. VaucherAbstractThe cholinergic, purinergic and oxidative stress systems were related to nervous system damage in some pathologies, as well as being involved in pro-inflammatory and anti-...
Sun, 11/24/2019 - 03:37
Author(s): Jiahui Liu, Zihe Li, Yanlei Cui, Haiyan Yang, Hu Shan, Chuanmei ZhangAbstractWe evaluated the phenotype and genotype of a fatal influenza/canine distemper virus coinfection found in farmed mink in China. We identified a novel subtype H1N1 influenza virus strain from the lungs of infected mink designated A/Mink/Shandong/1121/2017 (H1N1). The results of phylogenetic analysis of 8 gene...
Fri, 11/15/2019 - 03:21
Author(s): Zifeng Han, Li Li, Thomas Willer, Wolfgang Baumgärtner, Silke RautenschleinAbstractCampylobacter jejuni (C. jejuni) is a predominant cause of foodborne illness in humans, while its colonization in chickens is usually asymptomatic. Antibiotics are not routinely used to treat chickens against C. jejuni, but in the face of other bacterial diseases, C. jejuni may be exposed to antibiotics...

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