Bacillus cereus

Fri, 07/03/2020 - 16:00
Bacterial microorganisms which are latent in in vitro cultures can limit the efficiency of in vitro methods for the conservation of genetic resources. In this study we screened 2,373 accessions from the in vitro sweetpotato germplasm collection of the International Potato Center in Lima, Peru for bacteria associated with plantlets in tissue culture through a combination of morphological methods...
Fri, 07/03/2020 - 16:00
Bacillus cereus is one of the most important foodborne pathogenic microorganisms, which can lead to gastrointestinal and non-gastrointestinal diseases. However, the potential risk of B. cereus in aquatic products in China has not been comprehensively evaluated yet. In this study, a total of 860 aquatic samples from three types of retail aquatic products were collected from 39 major cities in...
Thu, 07/02/2020 - 08:02
by Jiajun Li, Wanqiang Li, Zhenhua Wang, Abdul Khalique, Junrui Wang, Miao Yang, Xueqin Ni, Dong Zeng, Dongmei Zhang, Yan Zeng, Qihui Luo, Bo Jing, Kangcheng Pan To explore the molecular mechanism of the effect of Bacillus cereus PAS38 on the immunity of broilers, sixty 7-day-old broilers were divided into two groups with three replicates. The control group was fed with basal diet, and the...
Wed, 07/01/2020 - 22:16
Eudragit RS100 nanoparticles containing Baccharis dracunculifolia DC essential oil (N‐EO) was developed by the nanoprecipitation method. N‐EO presented an average diameter of 151.6 nm, zeta potential (ζ) of +51.7 mV, and encapsulation efficiency of 99.4%. The antibacterial activity of N‐EO and free essential oil (EO) was evaluated in BHI broth against Staphylococcus aureus, Bacillus cereus,...
Tue, 06/23/2020 - 22:14
The objective of this study was to evaluate the possible synergistic interactions on antibacterial and antioxidant efficacy of two plant essential oils (EOs) Eucalyptus caesia Benth and Dracocephalum multicaule Montbr & Auch in vitro and in pear slices. Antibacterial combination effect was evaluated against seven important pathogenic bacteria (Bacillus cereus, Listeria monocytogenes,...
Mon, 06/22/2020 - 16:00
Incidences of heat stress due to the changing global climate can negatively affect the growth and yield of temperature-sensitive crops such as soybean variety, Pungsannamul. Increased temperatures decrease cro...
Sun, 06/21/2020 - 22:11
BACKGROUND The development of brewer's spent grain protein films with potential active packaging properties was investigated. Films were prepared by casting protein dispersions at different pHs (2, 8, 11), plasticizers (polyethylene glycol –PEG– or glycerol), and levels (0–0.25 g g−1) of PEG. Mechanical, water‐barrier and solubility, optical, antioxidant (reducing power, ABTS•+ and lipidic...
Tue, 06/16/2020 - 06:11
LWT
Author(s): Zekun Jin, Li Li, Yin Zheng, Peipei An This study aimed to apply garlic essential oil (GEO) to control the Bacillus cereus content in white sufu, a traditional Chinese fermented soybean curd. The effect of GEO on the Bacillus cereus count, bacterial composition, and the metabolic changes during the fermentation process were investigated. After treatment with GEO, B. cereus was reduced...
Fri, 06/12/2020 - 22:15
Bacterial toxins are food safety hazards causing about 10% of all reported foodborne outbreaks in Europe. Pertinent to Gram‐positive pathogens, the most relevant toxins are emetic toxin and diarrheal enterotoxins of Bacillus cereus, neurotoxins of Clostridium botulinum, enterotoxin of Clostridium perfringens, and a family of enterotoxins produced by Staphylococcus aureus and some other...
Sat, 06/06/2020 - 04:03
High moisture slurries used in production of table spreads may permit growth of  Staphylococcus aureus and  Bacillus cereus  and subsequent production of heat stable enterotoxin. Compliance with the Food Safety Modernization Act (FSMA), specifically 21 CFR Part 117, Subpart B and Section 117.8 (c)(2) and (c)(3) requires a hazard analysis to determine if preventive controls are needed. In this...
Wed, 06/03/2020 - 03:52
ABSTRACT Consumer demand for "fresh food" with no chemical preservatives has prompted researchers to pay more attention to natural antimicrobial peptides such as bacteriocins. Nisin is currently the most widely used food biopreservative among the bacteriocins; however, its applications are restricted due to its low stability at neutral and alkaline pH values. Circular bacteriocins have potent...
Fri, 05/29/2020 - 03:41
The present study was conducted to administer a commercial multi‐strain probiotic (MP), Yilibao (Bacillus velezensis:Bacillus cereus:Lactobacillus casei = 2:2:1), in Nile tilapia, Oreochromis niloticus. In terms of aerobic Bacillus spp. counts, we produced five diets containing 0, 0.34, 1.68, 3.36 and 6.72 g/kg of MP dry product (Control, T1, T2, T3, T4). Seven hundred and fifty tilapia juveniles...
Sun, 05/24/2020 - 03:37
The occurrence of multidrug-resistant pathogenic bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), multidrug-resistant Acinetobacter baumannii (MDRAB), extended-spectrum β-lactamase (ESBL) Escherichia coli, and Pseudomonas aeruginosa, has become a serious problem in animals and public. The objective of this study was to identify and isolate lactic acid bacterial (LAB) strains...
Sun, 05/24/2020 - 03:36
Background Human-use probiotics have recently been associated with clinical infections and antibiotic resistance transfer, raising public concern over their safety. However, despite their extensive application in aquaculture and animal husbandry, the safety of animal-use probiotics remains poorly described. Methods We evaluated the safety of 92 animal-use probiotics from China. The pattern of...
Fri, 05/22/2020 - 03:36
Streptomyces is the most frequently described genus of Actinomycetes, a producer of biologically active secondary metabolites. Indeed, the Streptomyces species produces about 70% of antibiotics and 60% of antifungal molecules used in agriculture. Our study was carried out with the goal of isolating and identifying antimicrobial secondary metabolites from Streptomyces misionensisV16R3Y1 isolated...
Thu, 05/21/2020 - 03:44
Bacillus cereus spores pose a challenge to the food industry owing to their ability to germinate in food during storage and produce toxins. The objective of the current study was to investigate the effect of pulsed electric field (PEF) at low electric field strength (i.e., 7.3, 8.1, 8.8 and 9.4 kV/cm) and moderate temperatures (65, 70, 75 and 80°C) on B. cereus spores. PEF treatment at 9.4 kV/cm...
Wed, 05/20/2020 - 03:47
Bacillus cereus sensu lato comprises Gram-positive spore-forming bacteria producing toxins associated with foodborne diseases. Three pore-forming enterotoxins, nonhemolytic enterotoxin (Nhe), hemolysin BL (Hbl), and cytotoxin K (CytK), are considered the primary factors in B. cereus sensu lato diarrhea. The aim of this study was to determine the potential risk of enterotoxicity among soil B....
Tue, 05/19/2020 - 03:40
Aims The objective of this study was to evaluate the antimicrobial effects of Radio frequency (RF) heating and the combination treatment of RF heating with ultraviolet (UV) radiation against foodborne pathogens in roasted grain powder (RGP). Methods and Results Foodborne pathogens inoculated on RGP were subjected to RF heating or RF‐UV combination treatments. After 120 s of RF heating, 4.68, 3.89...
Thu, 04/30/2020 - 03:43
Some biological activities of dihydroquercetin (DHQ), including antioxidative, antibacterial and anticancer activities, and the oxidative stability of butter oil supplemented with DHQ were evaluated. The DPPH* radical‐scavenging activity was high for DHQ, with an IC50 of 63.83 ± 3.11 μg/ml, compared to that of Trolox, which had an IC50 of 117.02 ± 5.19 μg/ml, over the concentrations studied (10–...
Thu, 04/16/2020 - 03:29
BACKGROUND Jiuqu are vital saccharifying and fermenting agents for Chinese fermented foods. Natural ventilation during Jiuqu fermentation causes changes in temperature, oxygen and moisture content, resulting in mass and heat gradients from the outer to inner areas of Jiuqu blocks. In the present study, microbiota stratification in Jiuqu was investigated by single molecule real‐time sequencing and...
Wed, 04/08/2020 - 03:32
Author(s): Anuj H. Chheda , Madhavi R. Vernekar An indigenously isolated Bacillus cereus isolated from soil was found to be capable of producing ε-poly-l-lysine (ε-PL), a homo-poly-amino acid of l-lysine which is used as a safe food preservative. The production of the ε-PL was optimized by identifying the most significant components of the medium which affect its production under shake-flask...
Wed, 04/08/2020 - 03:32
In this study, antagonistic effects of the chitinolytic enzyme producing isolates of Pseudomonas fluorescens PB27, Bacillus cereus B1 and Bacillus thuringiensis K1 against the aflatoxigenic fungus Aspergillus flavus were investigated. Germination of the spores of A. flavus was inhibited by up to nearly 20% after co-incubation with P. fluorescens PB27 for 72h, whereas the fungal spores were less...
Tue, 04/07/2020 - 03:49
Pepper bacterial wilt is caused by the bacterial pathogen, Ralstonia solanacearum. It is the most destructive disease of many Solanaceous crops such as potatoes, tobacco, pepper, tomatoes, and eggplant and is a significant source of crop loss worldwide. Physical, cultural and chemical controls have been employed to combat this destructive disease. However, none of these strategies has been able...
Thu, 04/02/2020 - 03:34
In dairy plants, clean-in-place (CIP) apparatus cannot be disassembled and, therefore, it is not easy to clean the inner surface of their pipes. In this study, the inhibitory effects of chemical agents on biofilms formed by three foodborne pathogens, Bacillus cereus , Escherichia coli , and Staphylococcus aureus , were evaluated in a dairy CIP system. First, the experiment was conducted on a...
Thu, 04/02/2020 - 03:33
This study examined the microbicidal activity of 222-nm UV radiation (UV222), which is potentially a safer alternative to the 254-nm UV radiation (UV254) that is often used for surface decontamination. Spores and/or growing and stationary-phase cells of Bacillus cereus, Bacillus subtilis, Bacillus thuringiensis, Staphylococcus aureus, and Clostridioides difficile and a herpesvirus were all killed...
Wed, 04/01/2020 - 03:27
Anti‐digestive enzyme, antioxidant, anti‐proliferative and antibacterial activities of the extract from Moringa oleifera at different extraction temperatures. Summary In an ultrasonic bath and for 30 min in the presence of water as extraction solvent in a ratio of 1:10 (g/mL), effect of extraction temperature on extraction of caffeoylquinic acids (CQAs) from Moringa oleifera leaves (MOL) was...
Thu, 03/26/2020 - 03:34
Rapid identification from positive blood cultures is standard of care (SOC) in many clinical microbiology laboratories. The GenMark Dx ePlex Blood Culture Identification Gram-Positive (BCID-GP) Panel is a multiplex nucleic acid amplification assay based on competitive DNA hybridization and electrochemical detection using eSensor technology. This multicenter study compared the investigational-use-...
Sat, 03/14/2020 - 03:30
Chlamydia psittaci is an important zoonotic pathogen and its oral route of infection plays an important role in the transmission and persistence. Bacillus cereus (B. cereus) strain, a common contaminant of animal feed and feedstuffs, can cause severe diarrhoea and malnutrition in poultry. In our previous study, a B. cereus strain (Dawu C), isolated from the haemorrhagic lungs of infected chickens...
Fri, 03/06/2020 - 03:53
Inactivation of Bacillales and Clostridiales spores is of interest, since some cause food spoilage and human diseases. A recent publication (Schlievert et al. 2018) reported that glycerol monolaurate (GML) in a non‐aqueous gel (GMLg) effectively killed spores of Bacillus subtilis, Bacillus cereus, and Clostridioides difficile, and Bacillus anthracis spores somewhat We now show that: 1) the B....
Wed, 03/04/2020 - 01:19
The microbiological quality of ready-to-eat (RTE) foods from school cafeterias in Chongqing, China, was evaluated in comparison to a guideline published by a provincial health commission. Based on their preparation processes and potential risks, the RTE foods were divided into 5 types: food type 1, general cooked and hot-held foods; food type 2, cooked meats; food type 3, heated aquatic products...
Sun, 03/01/2020 - 03:35
Author(s): J. Rocha-Pimienta, S. Martillanes, R. Ramírez, J. Garcia-Parra, J. Delgado-AdamezThe effect of high hydrostatic pressure (HHP) on pathogen microorganisms is influenced by an important number of variables like treatment conditions, microorganism type and food matrix, among others. This technology could be an alternative to pasteurization in Human Milk Banks. In this study, a response...
Fri, 02/28/2020 - 03:27
Microorganisms in dairy industries can form monospecies, dual-species, or multispecies biofilms, showing cooperative or competitive behaviors, which might contribute to the reduction of efficiency of cleaning and sanitization processes and eventually turn into a potential source of contamination. This study proposes to evaluate the behavior of Listeria monocytogenes in monospecies biofilms,...
Thu, 02/27/2020 - 03:46
This study was conducted to reveal the prevalence, molecular characterization, and antibiotic susceptibility of Bacillus cereus isolated from dairy products including powdered infant formula, raw milk, pasteurized milk, ultra-high-temperature milk, and cheese. Five hundred samples collected from 5 provinces in China were analyzed in overall experiments. Multilocus sequence typing, distribution of...
Wed, 02/26/2020 - 03:35
ABSTRACT The Bacillus cereus group comprises numerous closely related species, including bioterrorism agent B. anthracis, foodborne pathogen B. cereus, and biopesticide B. thuringiensis. Differentiating organisms capable of causing illness or death from those used in industry is essential for risk assessment and outbreak preparedness. However, current species definitions facilitate species-...
Sat, 02/15/2020 - 03:36
Author(s): Ryunkyoung Oh, Min Jeong Lee, Young-Ok Kim, Bo-Hye Nam, Hee Jeong Kong, Ju-Won Kim, Jung-yeon Park, Jung-Kil Seo, Dong-Gyun KimAbstractWe isolated and purified an antimicrobial peptide (AMP) from the mantle of the hard-shelled mussel, Mytilus coruscus. The peptide was purified through C18 reversed-phase high-performance liquid chromatography, and displayed antibacterial activity. Total...
Thu, 02/13/2020 - 03:45
Aims The aim of this study was to develop a novel selective agar for the specific isolation and detection of Bacillus anthracis. Methods and Results Based on published data on antibiotic resistance and susceptibility of B. anthracis and other closely related species of the Bacillus cereus sensu lato group, a new selective agar formulation termed CEFOMA (Bacillus CEreus sensu lato group specific...
Wed, 02/12/2020 - 10:50
Author(s): Shengqi Rao, Guangwei Xu, Xiangning Lu, Ruyi Zhang, Lu Gao, Qingyan Wang, Zhenquan Yang, Xinan JiaoAbstractCarvacrol is an effective natural antimicrobial and antioxidant agent; however, its poor aqueous solubility and high volatility limit its application in food systems. Ovalbumin can encapsulate hydrophobic molecules, thereby improving aqueous solubility and reducing volatility. The...
Tue, 02/11/2020 - 03:27
The study determined incidence, enterotoxigenecity and antimicrobial susceptibility profiles of Bacillus cereus isolated from ready-to-eat (RTE) milk products (n = 80), RTE meat products (n = 40), beverages (n = 40) and water samples (n = 60, from food preparing and serving outlets/restaurants) collected from eight different tourist places of Himachal Pradesh. 11.4% (25/220) samples were...
Mon, 02/03/2020 - 03:34
Author(s): Reza Roohi, Seyed Mohammad Bagher HashemiAbstractThe coupled 3D and transient form of heat and Maxwell's equations were solved to determine the temperature distribution within a carrot slice in the microwave cavity during pasteurization as a clean and energy saving process. To precisely simulate the turning of the sample, the dissipated thermal energy was applied to the carrot as a...
Mon, 02/03/2020 - 03:32
Author(s): Man-Seok Choi, Eun Bi Jeon, Ji Yoon Kim, Eun Ha Choi, Junsup Lim, Jinsung Choi, Shin Young ParkAbstractThis study investigated the effects of dielectric barrier discharge (DBD) plasma against Staphylococcus aureus and Bacillus cereus (initial inoculum of 8-9 log10CFU/mL) on the surface of dried blackmouth angler, and the physicochemical [pH, Hunter colors, thiobarbituric acid reactive...
Sat, 02/01/2020 - 04:00
Author(s): Hendrik Frentzel, Katharina Juraschek, Natalie Pauly, Ylanna Kelner-Burgos, Heidi Wichmann-SchauerAbstractMembers of the Bacillus cereus group are common contaminants of vegetables. One potential source of contamination is the application of B. thuringiensis based biopesticides. Although evidence of the presence of biopesticidal strains on food products is scarce, this information is...
Fri, 01/31/2020 - 03:29
Salmonella enterica, Listeria monocytogenes, Shigella flexneri, Escherichia coli O157:H7, Vibrio parahaemolyticus, Staphylococcus aureus, Vibrio cholerae, Clostridium botulinum type A, Bacillus cereus, Clostridium perfringens Alpha toxin, and Yersinia enterocolitica are 11 common foodborne pathogens. Traditional bacterial culture methods for detecting pathogens are time‐consuming and labor‐...
Thu, 01/23/2020 - 03:27
Aims This study was conducted to early detect the negative culture bacterial pathogens causing subclinical mastitis for the fast diagnosis of the disease and the reduction of some milk transmitted pathogenic bacteria to human consumers. Methods and Results A total of 171 positive California mastitis test (CMT) milk samples collected from asymptomatic dairy cows in Sharkia Governorate, Egypt were...
Wed, 01/15/2020 - 03:33
Author(s): A. Panigrahi, R.R. Das, M.R. Sivakumar, A. Saravanan, C. Saranya, N.S. Sudheer, K.P. Kumaraguru Vasagam, P. Mahalakshmi, S. Kannappan, G. GopikrishnaAbstractEffect of bio-augmentation of Bacillus spp in biofloc on growth, survival and immunity in Indian white shrimp Penaeus indicus was evaluated. Nine Bacillus strains were isolated and screened individually as well as in the form of a...
Wed, 01/15/2020 - 03:24
Bacillus cereus is widely distributed in different food products and can cause a variety of symptoms associated with food poisoning. Since ready-to-eat (RTE) foods are not commonly sterilized by heat treatment before consumption, B. cereus contamination may cause severe food safety problems. In this study, we investigated the prevalence of B. cereus in RTE food samples from different regions of...
Wed, 12/25/2019 - 03:33
Author(s): Jennifer A. Sánchez-Chica, Margarita M. Correa, Angel E. Aceves-Diez, Laura M. Castañeda-SandovalAbstractBacillus cereus is a major foodborne pathogen with the potential of causing emetic and diarrhoeal food poisoning. This study reports on the development of a sensitive and reliable method that involves direct DNA extraction from powdered milk and infant formula and a multiplex PCR...
Tue, 12/24/2019 - 03:35
by Jiajun Li, Wanqiang Li, Jianzhen Li, Zhenhua Wang, Dan Xiao, Yufei Wang, Xueqin Ni, Dong Zeng, Dongmei Zhang, Bo Jing, Lei Liu, Qihui Luo, Kangcheng Pan The aim of this study was to construct the spleen differential genes library of broilers fed with probiotic Bacillus cereus PAS38 by suppression subtractive hybridization (SSH) and screen the immune-related genes. Sixty seven-day-old broilers...
Tue, 12/24/2019 - 03:34
Quorum Sensing (QS) are mechanisms of synthesis and detection of signaling molecules to regulate gene expression and coordinate behaviors in bacterial populations. In Bacillus subtilis (Bs), multiple paralog Rap-Phr QS systems (receptor-signaling peptide) are highly redundant and multifunctional, interconnecting the regulation of differentiation processes such as sporulation and competence....
Tue, 12/24/2019 - 03:26
Aim Identification and enumeration of foodborne pathogens in food stuffs are valuable concerns. In the present study, starch‐blood‐egg yolk‐polymyxin B‐trimethoprim‐ceftazidime (SBYPTC) agar was established to isolate and specify the number of B. cereus in food products. Methods and results The effectiveness of the developed medium in selecting for B. cereus from pure cultures and food matrixes...
Sun, 12/15/2019 - 03:31
LWT
Author(s): Vijay K. Juneja, Marangeli Osoria, C.-A. Hwang, Abhinav Mishra, T. Matthew TaylorAbstractThe heat resistance of a four-stain cocktail of Bacillus cereus was determined in rice. Bags containing inoculated rice samples with hot water were submerged in a temperature-controlled water bath and held at 90.5, 95, or 99 °C for predetermined lengths of time. Surviving spore population were...

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