Campylobacter

Sat, 09/05/2020 - 14:08
The symptoms of infectious diarrheal disease are mediated by a combination of a pathogen’s virulence factors and the host immune system. Campylobacter jejuni is the leading bacterial cause of diarrhea worldwide due to its near-ubiquitous zoonotic association with poultry. One of the outstanding questions is to what extent the bacteria are responsible for the diarrheal symptoms via intestinal cell...
Fri, 08/28/2020 - 06:11
Author(s): Wageha A. Awad, Daniel Ruhnau, Claudia Hess, Michael Hess In recent years several studies emphasize on the deleterious effects of Campylobacter jejuni on the chicken intestine. In this context it was shown that Campylobacter jejuni, contrary to the general belief, has a negative influence on the gut barrier in chickens. More precisely, we demonstrated that C. jejuni affects gut...
Tue, 08/25/2020 - 10:05
ABSTRACT The crust is the outermost spore layer of most Bacillus strains devoid of an exosporium. This outermost layer, composed of both proteins and carbohydrates, plays a major role in the adhesion and spreading of spores into the environment. Recent studies have identified several crust proteins and have provided insights about their organization at the spore surface. However, although...
Mon, 08/24/2020 - 18:12
Aims: This study aimed to describe the demographic and geographic patterns of campylobacteriosis in Denmark, Finland, Norway and Sweden during 2000–2015. Methods: All Campylobacter infections notified to national authorities in the four countries during the study period were included. Background data for each notification consisted of patient age, sex, geographical location, presumed origin of...
Sat, 08/15/2020 - 12:00
Background Campylobacter infection is associated with impaired growth of children, even in the absence of symptoms. To examine the underlying mechanisms, we evaluated associations between Campylobacter infection, linear growth, and fecal microbial community features in a prospective birth cohort of 271 children with a high burden of diarrhea and stunting in the Amazonian lowlands of Peru. Methods...
Thu, 08/13/2020 - 16:00
Rapid developments in the field of whole genome sequencing (WGS) make in silico antimicrobial resistance (AMR) a target within reach. Campylobacter jejuni is a leading cause of foodborne infections in Israel with increasing rates of resistance. We applied WGS analysis to study the prevalence and genetic basis of AMR in 263 C. jejuni human and veterinary representative isolates retrieved from a...
Sat, 08/08/2020 - 04:02
The prevalence was determined of Campylobacter jejuni and C. coli on three age classes (lamb, hogget and mutton) of ovine carcass trim post-dressing and pre-chill. Sampling of hogget carcasses was undertaken six months before sampling of lamb and mutton carcasses. A total of 120 trim samples was collected from 11 processing plants across New Zealand (NZ). All samples were enriched and screened...
Tue, 08/04/2020 - 16:00
Campylobacteriosis is one of the most common causes of bacterial gastroenteritis. However, the clinical course of the illness varies in symptoms and severity. The aim of this study was to characterize Campylobacter jejuni (34 isolates) and C. coli (9 isolates) from persons with diarrheal and non-diarrheal stools at the time of examination and fecal sampling, in Poland by using whole-genome...
Tue, 08/04/2020 - 14:07
The aim of this study was to evaluate the influence of Lactobacillus reuteri B1/1 on the local intestinal immune response in the cecum of chickens experimentally infected by C. jejuni CCM6189. Day-old chicken were divided into four groups: control (C), L. reuteri B1/1 (LR), C. jejuni CCM6189 (CJ), and combined L. reuteri B1/1 + C. jejuni CCM6189 (LRCJ). Cecal samples were collected during...
Sun, 08/02/2020 - 06:13
LWT
Author(s): Wentao Jiang, KaWang Li, Yu-Chun Chiu, Carly Waldman, Cangliang Shen This study aimed to evaluate the thermal inactivation of Campylobacter jejuni in moisture enhanced reconstructed non-intact chicken patties with various pump rates and double pan-broiled at different temperatures. Fresh 1.5-kg coarse-ground chicken breast, inoculated with C. jejuni (3-strain mixture), were moisture...
Sat, 08/01/2020 - 06:19
Author(s): Jasmine Kataria, Sasikala Vaddu, Estefania Novoa Rama, Gaganpreet Sidhu, Harshavardhan Thippareddi, Manpreet Singh Peracetic acid (PAA) is commonly used as an antimicrobial during poultry processing to reduce the pathogen load on poultry and poultry products. However, limited research is available on the effects of pH on the efficacy of PAA against Salmonella and Campylobacter....
Fri, 07/31/2020 - 08:02
by Rhiannon L. Wallace, Dieter M. Bulach, Amy V. Jennison, Mary Valcanis, Angus McLure, James J. Smith, Trudy Graham, Themy Saputra, Simon Firestone, Sally Symes, Natasha Waters, Anastasia Stylianopoulos, Martyn D. Kirk, Kathryn Glass Australian rates of campylobacteriosis are among the highest in developed countries, yet only limited work has been done to characterize Campylobacter spp. in...
Thu, 07/30/2020 - 22:14
ABSTRACT Aims This study evaluated the performance of a commercial molecular detection method (mericon Campylobacter triple kit real‐time/quantitative (q)PCR) and a selective plating medium (R&F Campylobacter jejuni/Campylobacter coli Chromogenic Plating Medium (CCPM)) against a culture‐based reference method (ISO 10272‐1:2017 detection procedure B) for the detection of Campylobacter from raw...
Thu, 07/30/2020 - 04:02
Herein, a high-affinity single-stranded DNA aptamer (59 nt) against Campylobacter jejuni, defined as CJA1, was obtained using the whole-bacterium-based systemic evolution of ligands by exponential enrichment procedure. CJA1 was analyzed with a stable secondary structure and low dissociation constant (Kd) value of 1.37 ± 0.28 nM. The potential use of CJA1 was exemplified by the construction of a...
Thu, 07/23/2020 - 10:02
Campylobacter jejuni is the leading cause of bacterial foodborne disease worldwide. Here, we report the complete annotated genomes and plasmid sequences of 17 Campylobacter jejuni strains isolated from patients with gastroenteritis in Santiago, Chile.
Wed, 07/22/2020 - 08:02
Vertebrates, from zebra fish to humans, have an innate immune recognition of many bacterial flagellins. This involves a conserved eight-amino acid epitope in flagellin recognized by the Toll-like receptor 5 (TLR5). Several important human pathogens, such as Helicobacter pylori and Campylobacter jejuni, have escaped TLR5 activation by mutations in this...
Wed, 07/22/2020 - 00:00
Campylobacter species are among the major bacteria implicated in human gastrointestinal infections and are majorly found in faeces of domestic animals, sewage discharges and agricultural runoff. These pathogens have been implicated in diseases outbreaks through consumption of contaminated milk and water in some parts of the globe and reports on this is very scanty in the Eastern Cape Province....
Sat, 07/18/2020 - 08:02
The objective of this study was to compare the prevalence and antimicrobial resistance (AMR) rates of Campylobacter spp. isolated from conventional and organic turkey meat sold at retail in Germany. Samples of conventional (N = 527) and organic (N = 245) fresh turkey meat without skin were collected at retail markets throughout Germany and tested for Campylobacter spp.. Campylobacter isolates...
Sat, 07/11/2020 - 04:02
In Japan, strategies for ensuring food safety have been developed without reliable scientific evidence on the relation between foodborne infections and foods. The aim of this research was to provide information on the proportions of foodborne diseases caused by seven major causative pathogens ( Campylobacte r spp., Salmonella spp., EHEC, V. parahaemolyticus , Clostridium perfringens ,...
Fri, 07/10/2020 - 08:02
by Mihkel Mäesaar, Triin Tedersoo, Kadrin Meremäe, Mati Roasto The reservoir and source of human campylobacteriosis is primarily considered to be poultry, but also other such as ruminants, pets and environmental sources are related with infection burden. Multilocus sequence typing is often used for Campylobacter epidemiological studies to determine potential sources of human infections. The...
Fri, 07/10/2020 - 04:02
As one of the main causes of bacterial diarrhea and a major risk factor for triggering Guillain–Barré autoimmune syndrome, campylobacteriosis, that is, Campylobacter spp. infections, represents a major health issue worldwide. There is thus a pressing need for developing an effective and broad-coverage campylobacteriosis vaccine. Campylobacter jejuni, an encapsulated, multidrug resistant Gram-...
Thu, 07/09/2020 - 08:02
Campylobacter is well recognized as the leading cause of bacterial foodborne diarrheal disease worldwide with a very low outbreak reported in China. In May 2019, we investigated an outbreak of Campylobacter jejuni infections among students in a junior high school in Eastern China. Cases were interviewed to identify a common source of contamination. As cases were identified in the same school...
Thu, 07/09/2020 - 06:13
Author(s): C. Marin, S. Sevilla-Navarro, R. Lonjedo, P. Catalá-Gregori, M.A. Ferrús, S. Vega, A. Jiménez-Belenguer Thermophilic Campylobacter species are recognised as a major cause of acute bacterial diarrhoea in humans, being broiler meat the most common source of human infection. Antibiotic therapy is usually necessary for severe or prolonged infections, especially in immunocompromised...
Thu, 07/09/2020 - 00:00
Tea Tree oil (TTO) is well known for its numerous good properties but might be also irritating or toxic when used topically or ingested, thus limiting the number of possible applications in Humans. The aim of the study was to characterize the antimicrobial spectrum as well as the toxicity of Titroleane™, a new anti-infective agent obtained from TTO but cleared of its toxic monoterpenes...
Wed, 07/08/2020 - 04:02
Results from monitoring of the microbiological quality of 2,721 samples of ready-to-eat cooked chicken collected between 2013 to 2017 in England were reviewed: 70% of samples were from retail, catering or manufacture and 30% were imported and collected at English ports. Samples were tested for a range of bacterial pathogens and indicator organisms. Six samples (<1%) had unsatisfactory levels...
Sun, 07/05/2020 - 06:12
Author(s): Madeleine Moazzami, Lise-Lotte Fernström, Ingrid Hansson It is critical to maintain low levels of microbes in the whole food production chain. Due to high speed of slaughter, lack of time, and structural characteristics of crates, sufficient cleaning and disinfection of crates used for transporting chickens to abattoirs is a challenge. Inadequately cleaned transport crates for broiler...
Fri, 07/03/2020 - 08:02
by Eli J. Cohen, Daisuke Nakane, Yoshiki Kabata, David R. Hendrixson, Takayuki Nishizaka, Morgan Beeby Campylobacter jejuni rotates a flagellum at each pole to swim through the viscous mucosa of its hosts’ gastrointestinal tracts. Despite their importance for host colonization, however, how C. jejuni coordinates rotation of these two opposing flagella is unclear. As well as their polar placement...
Fri, 07/03/2020 - 06:10
Author(s): B.R. Wagle, A.M. Donoghue, S. Shrestha, I. Upadhyaya, K. Arsi, A. Gupta, R. Liyanage, N.C. Rath, D.J. Donoghue, A. Upadhyay Campylobacter jejuni is a major foodborne pathogen that causes gastroenteritis in humans. Chickens act as the reservoir host for C. jejuni, wherein the pathogen asymptomatically colonizes the ceca leading to contamination of carcasses during slaughter. The major...
Thu, 07/02/2020 - 06:14
Author(s): Ihab Habib, John Coles, Mark Fallows, Stan Goodchild Quantitative Microbiological Risk Assessment (QMRA) is a methodology used to organize and analyze scientific information to both estimate the probability and severity of an adverse event as well as prioritize efforts to reduce the risk of foodborne pathogens. No QMRA efforts have been applied to Campylobacter in the Australian...
Tue, 06/30/2020 - 10:03
ABSTRACT The most direct route by which microbes might assimilate sulfur would be by importing cysteine. However, alone among the amino acids, cysteine does not have well-characterized importers. We determined that Escherichia coli can rapidly import cysteine, but in our experiments, it did so primarily through the LIV ATP-driven system that is dedicated to branched-chain amino acids. The...
Fri, 06/26/2020 - 14:06
Mechanisms of host–pathogen interactions resulting in immunopathological responses upon human Campylobacter jejuni infection are not completely understood, but the recent availability of murine infection models mimicking key features of campylobacteriosis helps solving this dilemma. During a screen for proteases expressed by C. jejuni, we identified a peptidase of the M24 family as a potential...
Wed, 06/24/2020 - 10:02
Florfenicol belongs to a class of phenicol antimicrobials widely used as feed additives and for the treatment of respiratory infections. In recent years, increasing resistance to florfenicol has been reported in Campylobacter spp., the leading foodborne enteric pathogens causing diarrheal diseases worldwide. Here, we reported the identification of fexA, a novel mobile florfenicol resistance gene...
Tue, 06/23/2020 - 16:00
Campylobacter is a zoonotic pathogen that causes foodborne diarrheal illness globally. To better understand health risks in Southeastern China, Campylobacter spp. were surveyed in humans and representative poultry products over 3 years. One hundred and ninety-five representative isolates (n = 148, Campylobacter jejuni; n = 45, Campylobacter coli; n = 2 Campylobacter hyointestinalis) were examined...
Tue, 06/23/2020 - 10:02
Small noncoding RNAs (ncRNAs) are involved in many important physiological functions in pathogenic microorganisms. Previous studies have identified the presence of noncoding RNAs in the major zoonotic pathogen Campylobacter jejuni; however, few have been functionally characterized to date. CjNC110 is a conserved ncRNA in C. jejuni, located downstream of the luxS gene, which is responsible for the...
Mon, 06/22/2020 - 22:17
This study was carried out to investigate the frequency and genetic diversity of pestiviruses in abortion cases in cattle and small ruminants in Turkey. During January 2012 and December 2017, a total of 2029 aborted foetuses (553 bovine foetuses, 1388 sheep foetuses and 88 goat foetuses) were collected from different regions of Turkey. Real‐time RT‐PCR (RRT‐PCR) assays were used to detect...
Fri, 06/19/2020 - 16:00
Campylobacter jejuni, the most frequent cause of food-borne bacterial gastroenteritis, is a fastidious organism when grown in the laboratory. Oxygen is required for growth, despite the presence of the metabolic mechanism for anaerobic respiration. Amino acid auxotrophies are variably reported and energy metabolism can occur through several electron donor/acceptor combinations. Overall, the...
Thu, 06/18/2020 - 10:02
The microbiota plays a vital role in maintaining gut health and influences the overall performance of chickens. Most gut microbiota-related studies have been performed in broilers, which have different microbial communities compared to those of layers. The normal gut microbiota of laying chickens is dominated by Proteobacteria, Firmicutes, Bacteroidetes, Fusobacteria, and Actinobacteria at the...
Wed, 06/17/2020 - 16:00
Antimicrobial use in agricultural animals is known to be associated with increases in antimicrobial resistance. Most prior studies have utilized culture and susceptibility testing of select organisms to document these phenomena. In this study we aimed to detect 66 antimicrobial resistance (AMR) genes for 10 antimicrobial agent classes directly in swine fecal samples using our previously developed...
Wed, 06/17/2020 - 16:00
Campylobacter fetus is commonly associated with venereal disease and abortions in cattle and sheep, and can also cause intestinal or systemic infections in humans that are immunocompromised, elderly, or exposed to infected livestock. It is also believed that C. fetus infection can result from the consumption or handling of contaminated food products, but C. fetus is rarely detected in food since...
Wed, 06/17/2020 - 16:00
Raw milk is a frequent vehicle for transmission of thermophilic Campylobacter, leading to reported outbreaks. Milk is a challenging food matrix for pathogen detection, due to its high protein and lipid content. Limited detection of Campylobacter colony-forming unit (CFU) in raw milk might underestimate the pathogen’s infectious potential. We optimized a viability real-time PCR (qPCR) for...
Tue, 06/09/2020 - 22:16
Campylobacter is the most common cause of bacterial gastroenteritis worldwide and the most frequently reported foodborne pathogen in the European Union (EU). The predominant source of infection is poultry. The reduction of Campylobacter spp. in the poultry meat chain in the EU can only be achieved with an integrated meat safety assurance approach. Abstract Campylobacter is the most common cause...
Tue, 06/09/2020 - 00:00
Campylobacteriosis is the leading cause of human bacterial gastroenteritis, very often associated with poultry consumption. Thermophilic Campylobacter (Campylobacter jejuni and Campylobacter coli) isolates (n = 158) recovered from broiler neck skin and caecal contents in Ireland over a one-year period, resistant to at least one of three clinically relevant antimicrobial classes, were screened for...
Wed, 06/03/2020 - 03:52
It is important for the poultry industry to maximize product safety and quality by understanding the connection between bacterial diversity on chicken carcasses throughout poultry processing to the end of shelf life and the impact of the local processing environment. Enumeration of total aerobic bacteria, Campylobacter and Pseudomonas, and 16S rRNA gene amplicon sequencing were used to evaluate...
Sat, 05/30/2020 - 03:47
Understanding consumers’ perceptions toward chicken meat safety and quality could provide valuable information to public health educators since it is the most consumed meat. This study explores perceptions of a group of South African consumers on the safety and quality of chicken meat based on intrinsic and extrinsic attributes and identifies related safety risks. Data were collected through a...
Wed, 05/27/2020 - 03:48
Campylobacter jejuni is one of the leading causes of bacterial gastroenteritis worldwide. In the United States, New Hampshire was one of the 18 states that reported cases in the 2016 to 2018 multistate outbreak of multidrug-resistant C. jejuni. Here, we aimed to elucidate the baseline diversity of the wider New Hampshire C. jejuni population during the outbreak. We used genome sequences of 52...
Wed, 05/27/2020 - 03:36
Campylobacter jejuni monitors intestinal metabolites produced by the host and microbiota to initiate intestinal colonization of avian and animal hosts for commensalism and infection of humans for diarrheal disease. We previously discovered that C. jejuni has the capacity to spatially discern different intestinal regions by sensing lactate and the short-chain...
Fri, 05/22/2020 - 03:47
Bacteria of the genus Campylobacter are major foodborne pathogens that have become increasingly resistant to clinically important antimicrobial agents (1). In China, Campylobacter jejuni is the main cause of human Campylobacter infections, whereas Campylobacter coli isolates exhibit a distinctly higher multidrug resistance rate. Fluoroquinolones and macrolides are the most frequently used...
Fri, 05/22/2020 - 03:39
Aim To investigate factors influencing Campylobacter spp. colonization of broiler chickens. Methods and Results Campylobacters were isolated from caeca from 319 flocks of two different breeds (199 Cobb and 120 Hubbard), reared as standard (199), Freedom Food/corn fed (57), free‐range (47) or organic (16). The standard category exclusively used Cobb birds slaughtered at 38‐41 days. The Freedom...
Wed, 05/20/2020 - 03:35
Modern agriculture has dramatically changed the distribution of animal species on Earth. Changes to host ecology have a major impact on the microbiota, potentially increasing the risk of zoonotic pathogens being transmitted to humans, but the impact of intensive livestock production on host-associated bacteria has rarely been studied. Here, we...
Tue, 05/19/2020 - 03:36
Lipooligosaccharide (LOS) is an integral component of the Campylobacter cell membrane with a structure of core oligosaccharides forming inner and outer core regions and a lipid A moiety. The gene content of the LOS core biosynthesis cluster exhibits extensive sequence variation, which leads to the production of variable cell surface LOS structures in Campylobacter. Some LOS outer core molecules...

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