Campylobacter

Tue, 10/04/2016 - 04:01
Summary Aim“Label Rouge” broiler free-range carcasses have been monitored since 1991, and broiler flocks since 2010, for contamination by the main foodborne zoonotic bacteria. Material and MethodsInitially the monitoring plan mainly focused on the surveillance of Salmonella, and on indicators of the overall microbiological quality of free-range broiler carcasses such as S. aureus and coliforms,...
Sat, 10/01/2016 - 01:14
Islam I. Sabike, Ryoko Uemura, Yumi Kirino, Hirohisa Mekata, Satoshi Sekiguchi, Tamaki Okabayashi, Yoshitaka Goto, Wataru Yamazaki
Fri, 09/30/2016 - 11:00
Our study investigated the antimicrobial action of clove (Syzygium aromaticum) essential oil (EO) on the zoonotic pathogen Campylobacter jejuni. After confirming the clove essential oil's general antibacterial effect, we analyzed the reference strain Campylobacter jejuni NCTC 11168. Phenotypic, proteomic, and transcriptomic methods were used to reveal changes in cell morphology and functions when...
Fri, 09/30/2016 - 11:00
Peptidoglycan (PG) acetylation of Gram-positive bacteria confers lysozyme resistance and contributes to survival in the host. However, the importance of PG acetylation in Gram-negative bacteria has not been fully elucidated. The genes encoding putative PG acetyltransferase A (PatA) and B (PatB) are highly conserved in Campylobacter jejuni, the predominant cause of bacterial diarrhea worldwide. To...
Wed, 09/28/2016 - 04:27
Abstract A semi-quantitative ecometric technique was used to evaluate efficacy of selective culture media. It involved a standardized chronological streaking technique, leading to ever-decreasing number of colonies. The objective was to evaluate the efficacy of this technique (four-quandrant sequential streak [FQS]) with two commonly used Campylobacter broths (Bolton's and Tecra) with whole...
Wed, 09/21/2016 - 20:00
The emerging enteric pathogen Campylobacter concisus is associated with prolonged diarrhea and inflammatory bowel disease. Previous studies have shown that C. concisus strains are very genetically diverse. Nevert...
Tue, 09/20/2016 - 11:46
Infections due to clonal expansion of highly virulent bacterial strains are clear and present threats to human and animal health. Association of genetic changes with disease is now a routine, but identification of causative mutations that enable disease remains difficult. Campylobacter jejuni is an important zoonotic pathogen transmitted to humans...
Sat, 09/17/2016 - 17:23
Publication date: 5 December 2016 , Volume 238 Author(s): Jinsong Feng, Guillaume Lamour, Rui Xue, Mehr Negar Mirvakliki, Savvas G. Hatzikiriakos, Jie Xu, Hongbin Li, Shuo Wang, Xiaonan Lu Campylobacter jejuni is a microaerophilic pathogen and leading cause of human gastroenteritis. The presence of C. jejuni encased in biofilms found in meat and poultry processing...
Thu, 09/08/2016 - 19:11
For a long time Campylobacter was only considered as a commensal microorganism in avian hosts restricted to the ceca, without any pathogenic features. The precise reasons for the symptomless chicken carriers are still unknown, but investigations of the gastrointestinal ecology of broiler chickens may improve our understanding of the microbial interactions with the host. Therefore, the current...
Tue, 09/06/2016 - 00:00
Campylobacter is associated with live broilers and chicken meat products. There is some discussion in the literature about the possibility that Campylobacter prevalence in broilers could be affected by season or weather conditions. The objective of this study was to measure the flock prevalence of Campylobacter by sampling cecal contents from multiple flocks in one commercial slaughter plant over...
Mon, 08/08/2016 - 17:00
Marta Isabel Gracia, Jaime Sánchez, Carlos Millán, Óscar Casabuena, Peter Vesseur, Ángel Martín, Francisco Javier García-Peña, Pedro Medel Two independent trials were carried out to evaluate the effect of feed form, whole wheat (WW) and oat hulls (OH) addition on gastrointestinal (GIT) weight and Campylobacter jejuni colonization in orally infected birds. In Trial 1, there were six treatments...
Fri, 08/05/2016 - 02:00
Summary Using data collected from a cross-sectional study of 25 farms (eight beef, eight swine and nine dairy) in 2010, we assessed clustering of molecular subtypes of C. jejuni based on a Campylobacter-specific 40 gene comparative genomic fingerprinting assay (CGF40) subtypes, using unweighted pair-group method with arithmetic mean (UPGMA) analysis, and multiple correspondence analysis. Exact...
Mon, 08/01/2016 - 11:20
Despite the importance of lipooligosaccharides (LOS) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in C. coli. In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species. Furthermore, we paired C. coli genomic information and...
Wed, 07/27/2016 - 08:25
Summary The objectives of this study were to (i) compare the carriage of Campylobacter and antimicrobial-resistant Campylobacter among livestock and mammalian wildlife on Ontario farms, and (ii) investigate the potential sharing of Campylobacter subtypes between livestock and wildlife. Using data collected from a cross-sectional study of 25 farms in 2010, we assessed associations, using mixed...
Fri, 07/22/2016 - 17:33
The turkey industry relies totally on artificial insemination to continue and improve production. If something compromises the insemination process, such as contaminated semen, it becomes a detrimental loss to the industry. Bacteria have been found in broiler breeder males to reduce sperm motility. The Sperm Quality Index (SQI) is a quick method to determine avian sperm motility using the sperm...
Thu, 07/21/2016 - 11:58
The PrePex circumcision device causes ischemic necrosis of the foreskin, raising concerns of anaerobic overgrowth. We compared the subpreputial microbiome of 2 men 7 days after PrePex device placement to that of 145 uncircumcised men in Rakai, Uganda, using 16S ribosomal (rRNA) RNA gene–based quantitative polymerase chain reaction analysis and sequencing. PrePex users had higher absolute...
Wed, 07/20/2016 - 17:00
Greta Gölz, Thomas Alter, Stefan Bereswill, Markus M. Heimesaat Background Only limited information is available about the immunopathogenic properties of Arcobacter infection in vivo. Therefore, we performed a meta-analysis of published data in murine infection models to compare the pathogenic potential of Arcobacter butzleri with Campylobacter jejuni and commensal Escherichia coli as pathogenic...
Tue, 07/19/2016 - 10:02
Publication date: 3 October 2016 , Volume 234 Author(s): T. Seliwiorstow, L. De Zutter, K. Houf, N. Botteldoorn, J. Baré, I. Van Damme The performance of different isolation methods was evaluated for the detection of Campylobacter from naturally contaminated raw poultry meat. Therefore, fresh and frozen poultry meat samples were analysed using the standard procedure (ISO 10272-1:...
Fri, 07/15/2016 - 11:01
Campylobacter spp. are the leading cause of bacterial gastroenteritis worldwide, and water is increasingly seen as a risk factor in transmission. Here we describe a most-probable-number (MPN)–quantitative PCR (qPCR) assay in which water samples are centrifuged and aliquoted into microtiter plates and the bacteria are enumerated by qPCR. We observed that commonly used Campylobacter molecular...
Fri, 07/15/2016 - 11:01
Campylobacter jejuni is a foodborne pathogen that often leads to human infections through the consumption of contaminated poultry. Wild birds may play a role in the transmission of C. jejuni by acting as reservoir hosts. Despite ample evidence that wild birds harbor C. jejuni, few studies have addressed the role of host ecology in transmission to domestic animals or humans. We tested the...
Fri, 07/15/2016 - 03:20
Xueqing Du, Nan Wang, Fangzhe Ren, Hong Tang, Xinan Jiao, Jinlin Huang
Fri, 07/15/2016 - 00:00
Cytolethal distending toxin (CDT) is found in Gram-negative bacteria, especially in certain Proteobacteria such as the Pasteurellaceae family, including Haemophilus ducreyi and Aggregatibacter (Actinobacillus) actinomycetemcomitans, in the Enterobacteriaceae family and the Campylobacterales order, including the Campylobacter and Helicobacter species. In vitro and in vivo studies have clearly...
Thu, 07/14/2016 - 03:20
Tara Grinnage-Pulley, Yang Mu, Lei Dai, Qijing Zhang
Fri, 07/08/2016 - 17:00
Evgeny A. Idelevich, Karsten Becker, Janne Schmitz, Dennis Knaack, Georg Peters, Robin Köck Results of disk diffusion antimicrobial susceptibility testing depend on individual visual reading of inhibition zone diameters. Therefore, automated reading using camera systems might represent a useful tool for standardization. In this study, the ADAGIO automated system (Bio-Rad) was evaluated for...
Wed, 07/06/2016 - 22:07
Publication date: January 2017 , Volume 71 Author(s): Se-Hun Kim, Changwon Park, Eun-Jung Lee, Woo-Suk Bang, Yun-Ji Kim, Joo-Sung Kim Campylobacter is a well-known bacterial agent that causes foodborne gastroenteritis. Its biofilm-forming ability is known to be important for its survival in harsh conditions. Poultry is a main reservoir of Campylobacter. In this study, we...
Thu, 06/30/2016 - 11:01
Campylobacteriosis is one of the most important foodborne diseases worldwide and a significant health burden in New Zealand. Campylobacter jejuni is the predominant species worldwide, accounting for approximately 90% of human cases, followed by Campylobacter coli. Most studies in New Zealand have focused on C. jejuni; hence, the impact of C. coli strains on human health is not well understood....
Thu, 06/30/2016 - 11:01
Animals are important reservoirs of zoonotic enteropathogens, and transmission to humans occurs more frequently in low- and middle-income countries (LMICs), where small-scale livestock production is common. In this study, we investigated the presence of zoonotic enteropathogens in stool samples from 64 asymptomatic children and 203 domestic animals of 62 households in a semirural community in...
Thu, 06/30/2016 - 03:20
Vicky Bronnec, Hana Turoňová, Agnès Bouju, Stéphane Cruveiller, Ramila Rodrigues, Katerina Demnerova, Odile Tresse, Nabila Haddad, Monique Zagorec
Wed, 06/29/2016 - 04:50
Abstract Bacterial flagella assembly is tightly regulated to ensure a timely and sequential production of the various flagellum constituents. In the pathogen Campylobacter jejuni the hierarchy in flagella biosynthesis is largely determined at the transcriptional level through the activity of the alternative sigma factors sigma54 and sigma28. Here we report that C. jejuni flagellin levels are also...
Thu, 06/23/2016 - 05:15
Summary In May 2012, an outbreak of campylobacteriosis occurred in southern Sweden at a wedding reception affecting 44 persons. A total of 17 cases were notified (13 were culture positive for Campylobacter spp.). Epidemiological investigation suspected chicken liver pâté as the source of infection. The liver pâté had been deliberately undercooked, lightly fried to keep the right texture and mixed...
Mon, 06/20/2016 - 17:00
Stefan Bereswill, Marie E. Alutis, Ursula Grundmann, André Fischer, Ulf B. Göbel, Markus M. Heimesaat Background Human Campylobacter jejuni infections are progressively rising worldwide. Information about the molecular mechanisms underlying campylobacteriosis, however, are limited. In the present study we investigated whether cytokines such as IL-23, IL-22 and IL-18, which share pivotal functions...
Mon, 06/20/2016 - 00:00
Abstract Gut microbiota is important to human health. Specific dietary glycans promote favorable microbiota growth and inhibit pathobionts. Dietary glycans most relevant to adults and weaned infants are derived from plants or lactose; human milk oligosaccharides (HMOS) are most relevant to breastfed infants. Their efficacy in supporting bacterial growth is compared to determine...
Thu, 06/16/2016 - 14:01
Publication date: Available online 15 June 2016 Author(s): Krunoslav Bojanić, Anne Camilla Midwinter, Jonathan Craig Marshall, Lynn Elizabeth Rogers, Patrick Jon Biggs, Els Acke Campylobacter enteritis in humans is primarily associated with C. jejuni/coli infection. The impact of other Campylobacter spp. is likely to be underestimated due to the bias of culture methods towards...
Thu, 06/16/2016 - 13:59
Publication date: 16 August 2016 , Volume 231 Author(s): Hamzah M. Al-Qadiri, Murad A. Al-Holy, Setareh Ghorban Shiroodi, Mahmoudreza Ovissipour, Byju N. Govindan, Nivin Al-Alami, Shyam S. Sablani, Barbara Rasco The effect of acidic electrolyzed water (AEW) on inactivating Escherichia coli O104:H4, Listeria monocytogenes, Aeromonas hydrophila, Vibrio parahaemolyticus and...
Tue, 06/14/2016 - 17:25
Abstract Campylobacter jejuni is an important pathogen of significant public health importance. This pathogen is associated with human infection and has been isolated from mammals and birds. Ninety-two cloacal C. jejuni isolates were identified from 35 layer farms in Northern Jordan. Antimicrobial susceptibility was determined using minimal inhibitory concentration (MIC) and disc diffusion...
Mon, 06/13/2016 - 07:20
Abstract Toxin-antitoxin (TA) systems are widely distributed in bacteria and play an important role in maintaining plasmid stability. The leading foodborne pathogen, Campylobacter jejuni, can carry multiple plasmids associated with antibiotic resistance or virulence. Previously a virulence plasmid named pVir was identified in C. jejuni 81-176 and IA3902, but determining the role of pVir in...
Mon, 06/13/2016 - 03:20
Elisabetta Di Giannatale, Giuliano Garofolo, Alessandra Alessiani, Guido Di Donato, Luca Candeloro, Walter Vencia, Lucia Decastelli, Francesca Marotta
Sun, 06/12/2016 - 11:18
Publication date: December 2016 , Volume 70 Author(s): Dean Burfoot, Jeremy Hall, Keith Nicholson, Kirsty Holmes, Cedric Hanson, Simon Handley, Elizabeth Mulvey These studies examined the effect of rapid surface cooling on the numbers of Campylobacter on chicken carcasses. In two trials, chicken skins were immersed in liquid nitrogen. Campylobacter numbers were reduced by 1...
Wed, 06/01/2016 - 03:20
Christian Penny, Beau Grothendick, Lin Zhang, Connie M. Borror, Duane Barbano, Angela J. Cornelius, Brent J. Gilpin, Clifton K. Fagerquist, William J. Zaragoza, Michele T. Jay-Russell, Albert J. Lastovica, Catherine Ragimbeau, Henry-Michel Cauchie, Todd R. Sandrin
Tue, 05/31/2016 - 11:00
Bacterial community composition and longitudinal fluctuations were monitored in a riverine system during and after Superstorm Sandy to better characterize inter- and intracommunity responses associated with the disturbance associated with a 100-year storm event. High-throughput sequencing of the 16S rRNA gene was used to assess microbial community structure within water samples from Muddy Creek...
Sat, 05/28/2016 - 04:55
Summary Campylobacter jejuni is one of the major causative pathogens of outbreaks or sporadic cases of diarrhoeal diseases worldwide. In this study, we compared the phenotypic and genetic characteristics of C. jejuni isolates of human and food-producing animal origins in Korea and examined the genetic relatedness between these two groups of isolates. Regardless of isolation source, all C. jejuni...
Sat, 05/28/2016 - 04:33
Publication date: December 2016 , Volume 70 Author(s): Nematollah Jonaidi-Jafari, Faham Khamesipour, Reza Ranjbar, Roohollah Kheiri Campylobacter is one of the most important food borne pathogens that cause bacterial gastroenteritis worldwide. The most commonly isolated species in humans are Campylobacter jejuni (C. jejuni) and Campylobacter coli. The emergence of antimicrobial...
Tue, 05/24/2016 - 12:13
To acquire essential Fe(III), bacteria produce and secrete siderophores with high affinity and selectivity for Fe(III) to mediate its uptake into the cell. Here, we show that the periplasmic binding protein CeuE of Campylobacter jejuni, which was previously thought to bind the Fe(III) complex of the hexadentate siderophore enterobactin (Kd...
Sat, 05/21/2016 - 04:23
Publication date: November 2016 , Volume 69 Author(s): Tara Battersby, P. Whyte, D. Bolton The objective of this study was to test the hypothesis that farm staff are the primary vector of Campylobacter transmission into broiler flocks. On 3 different farms and 5 different flocks (3 flocks on farm 1 and 1 flock on each of farms 2 and 3) a small section of the broiler house (3 × 2 m (farm...
Sat, 05/21/2016 - 03:00
Abstract AimsTo determine the genetic diversity of Campylobacter jejuni and Campylobacter coli isolates from Nigeria and to identify the association between multilocus sequence types and hosts (poultry, cattle and humans). Methods and ResultsIsolates were identified using multiplex PCR assays. Multilocus sequence typing (MLST) was used to determine the genetic diversity of 36 Campylobacter jejuni...

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