Campylobacter

Wed, 05/18/2016 - 02:30
Publication date: November 2016 , Volume 69 Author(s): Alexander Rohde, Jens Andre Hammerl, Sascha Al Dahouk The efficient and timely detection of bacterial pathogens remains a major public health concern throughout the world. Fluorescence in situ hybridization (FISH) is a promising tool to detect bacteria since it incorporates the advantages of rapid detection methods with the live/...
Sat, 05/14/2016 - 00:25
Summary This study investigated the ecological distribution of zoonotic bacterial pathogen, Campylobacter, in mixed crop–livestock (MCL) farms compared to conventional farms and their products at pre- and post-harvest levels. A total of 222 Campylobacter isolates were identified. At pre-harvest level, a total of 1287 samples from seven MCL farms, four conventional poultry farms, four organic...
Fri, 05/06/2016 - 10:14
Publication date: November 2016 , Volume 69 Author(s): Xinfeng Han, Dongmei Zhu, Haimei Lai, Hang Zeng, Kang Zhou, Likou Zou, Congming Wu, Guoquan Han, Shuliang Liu A total of 651 samples from broiler cecal samples, carcasses, carcass parts collected at the slaughterhouse level in Sichuan Province of China were examined for the occurrence of Campylobacter jejuni and...
Wed, 05/04/2016 - 22:37
LWT
Publication date: October 2016 Source:LWT - Food Science and Technology, Volume 72 Author(s): Juliane Alves, Elisa Yoko Hirooka, Tereza Cristina Rocha Moreira de Oliveira Conventional methods for the detection of Campylobacter and Salmonella based on culturing are time consuming and laborious. The aim of this study was to develop a multiplex real-time PCR assay with an internal...
Tue, 05/03/2016 - 10:46
Abstract Campylobacter jejuni and Campylobacter coli are zoonotic pathogens once considered asaccharolytic, but are now known to encode pathways for glucose and fucose uptake/metabolism. For C. jejuni, strains with the fuc locus possess a competitive advantage in animal colonization models. We demonstrate that this locus is present in >50% of genome-sequenced strains and is prevalent in...
Wed, 04/27/2016 - 03:20
Wonhee Cha, Rebekah Mosci, Samantha L. Wengert, Pallavi Singh, Duane W. Newton, Hossein Salimnia, Paul Lephart, Walid Khalife, Linda S. Mansfield, James T. Rudrik, Shannon D. Manning
Mon, 04/25/2016 - 11:00
Culture-independent detection tests (CIDTs) for Campylobacter have become an area of intense controversy and confusion among laboratorians in the field of clinical microbiology. To date, the true analytical and clinical performance of stool antigen CIDTs versus truly optimized culture conditions is unknown. In this issue of the Journal of Clinical Microbiology, Fitzgerald and colleagues (C....
Mon, 04/25/2016 - 11:00
The use of culture-independent diagnostic tests (CIDTs), such as stool antigen tests, as standalone tests for the detection of Campylobacter in stool is increasing. We conducted a prospective, multicenter study to evaluate the performance of stool antigen CIDTs compared to culture and PCR for Campylobacter detection. Between July and October 2010, we tested 2,767 stool specimens from patients...
Wed, 04/20/2016 - 22:27
Publication date: October 2016 , Volume 68 Author(s): Ewa Pacholewicz, Sri Aika Sura Barus, Arno Swart, Arie H. Havelaar, Len J.A. Lipman, Pieternel A. Luning Campylobacter remains the most commonly reported zoonotic agent worldwide. Reducing the concentration of Campylobacter on chicken meat is seen as the most efficient strategy to diminish the number of human...
Mon, 04/18/2016 - 11:01
Campylobacter jejuni is the leading cause of bacterial gastroenteritis worldwide. Transmission to humans occurs through consumption of contaminated food or water. The conditions affecting the persistence of C. jejuni in the environment are poorly understood. Some protozoa package and excrete bacteria into multilamellar bodies (MLBs). Packaged bacteria are protected from deleterious conditions,...
Sat, 04/09/2016 - 06:40
Summary Campylobacter spp. are important causes of bacterial zoonosis, most often transmitted by contaminated poultry meat. From an epidemiological and risk assessment perspective, further knowledge should be obtained on Campylobacter prevalence and genotype distribution in primary production. Consequently, 15 Austrian broiler flocks were surveyed in summer for their thermophilic Campylobacter...
Fri, 04/08/2016 - 20:48
Publication date: September 2016 , Volume 58 Author(s): Arthur Hinton The ability of Campylobacter to grow aerobically in media supplemented with fumarate-pyruvate or with dairy, meat, or soy extracts or peptones was examined. Optical densities (OD) of Campylobacter cultured in basal media, media supplemented with fumarate-pyruvate or with 1.0, 2.5, 5.0, or 7.5% beef extract was measured....
Thu, 04/07/2016 - 23:58
Limitations in quality bedding material have resulted in the growing need to re-use litter during broiler farming in some countries, which can be of concern from a food-safety perspective. The aim of this study was to compare the Campylobacter levels in ceca and litter across three litter treatments under commercial farming conditions. The litter treatments were (a) the use of new litter after...
Thu, 04/07/2016 - 23:58
The housing of laying hens is important for social, industrial, and regulatory aspects. Many studies have compared hen housing systems on the research farm, but few have fully examined commercial housing systems and management strategies. The current study compared hens housed in commercial cage-free aviary, conventional cage, and enriched colony cage systems. Environmental and eggshell pool...
Thu, 04/07/2016 - 23:58
The efficacy of the commercial competitive exclusion product Broilact against Campylobacter jejuni was evaluated in broiler chickens in a 5-week pilot-scale study. Newly-hatched broiler chicks were brought from a commercial hatchery. After arrival 50 seeder chicks were challenged orally with approximately 103 cfu of C. jejuni, wing marked, and placed back in a delivery box and moved to a separate...
Mon, 04/04/2016 - 11:01
The genetic diversity of Campylobacter jejuni and Campylobacter coli isolates from commercial broiler farms was examined by multilocus sequence typing (MLST), with an assessment of the impact of the sample type and laboratory method on the genotypes of Campylobacter isolated. A total of 645 C. jejuni and 106 C. coli isolates were obtained from 32 flocks and 17 farms, with 47 sequence types (STs)...
Mon, 04/04/2016 - 11:01
Foodborne illnesses associated with pathogenic bacteria are a global public health and economic challenge. The diversity of microorganisms (pathogenic and nonpathogenic) that exists within the food and meat industries complicates efforts to understand pathogen ecology. Further, little is known about the interaction of pathogens within the microbiome throughout the meat production chain. Here, a...
Thu, 03/31/2016 - 01:11
Abstract The study was conducted to determine the level of Salmonella, Escherichia coli, Enterobacteriaceae and aerobic colony counts (ACCs) contamination in beef carcass, equipment, slaughtermen hands and water at selected stages in abattoirs. Cattle carcasses were sampled at four sites (rump, neck, flank and brisket) from a low throughput (LTA) (n = 240) and high throughput (HTA) (n = 384)...
Tue, 03/29/2016 - 17:00
Abstract Bactericidal activity of neutral electrolyzed water (NEW), quaternary ammonium (QUAT), and lactic acid-based solutions was investigated using a manual spraying technique against Salmonella Typhimurium, Escherichia coli O157:H7, Campylobacter jejuni, Listeria monocytogenes and Staphylococcus aureus that were inoculated onto the surface of scarred polypropylene and wooden food cutting...
Tue, 03/29/2016 - 12:15
Although it is known that diverse bacterial flagellar motors produce different torques, the mechanism underlying torque variation is unknown. To understand this difference better, we combined genetic analyses with electron cryo-tomography subtomogram averaging to determine in situ structures of flagellar motors that produce different torques, from Campylobacter and Vibrio species....
Mon, 03/28/2016 - 17:05
The purpose of the present study was to evaluate a technique involving the use of commercially available FTA classic card (Whatman) for transporting and detection of DNA to use in PCR analysis and genetic sequencing of Campylobacter jejuni of poultry origin. Fifty isolates of Campylobacter jejuni were obtained from broiler carcasses in Rio Grande do Sul, Brazil. Antimicrobial susceptibility...
Mon, 03/28/2016 - 17:05
A total of 636 day-of-hatch Ross 308 broilers chicks were used in 4 independent trials carried out to screen the effect of 12 feed additives on reducing cecal colonization of Campylobacter in broilers. The tested additives were probiotics based on B. subtilis and S. cerevisae, a garlic extract, a blend of herbal substances and essential oils, two different combinations of essential oils and...
Fri, 03/25/2016 - 11:01
Diarrheal disease is a complex syndrome that remains a leading cause of global childhood morbidity and mortality. The diagnosis of enteric pathogens in a timely and precise manner is important for making treatment decisions and informing public health policy, but accurate diagnosis is a major challenge in industrializing countries. Multiplex molecular diagnostic techniques may represent a...
Thu, 03/24/2016 - 00:57
Publication date: September 2016 , Volume 67 Author(s): Nelly Denis, Helen Zhang, Alexandre Leroux, Roger Trudel, Henri Bietlot In recent years, fresh fruits and vegetables have been linked to numerous foodborne illness outbreaks in different regions of the world, including in Canada. In light of rising concerns over the microbial safety of these commodities, the Canadian Food...
Wed, 03/23/2016 - 02:40
Abstract AimTo elucidate the distribution and circulation dynamics of Campylobacter and Salmonella in Japanese chicken broiler flocks. Methods and ResultsA two-year investigation of the distribution of Campylobacter and Salmonella was conducted in 25 broiler flocks at 9 farms in Japan from 2013 to 2014. Campylobacter and Salmonella tested positive in 11 (44.0%) and 24 (96.0%) broiler flocks,...
Tue, 03/22/2016 - 02:45
e-First Articles. Campylobacter spp. are a substantial cause of gastroenteritis worldwide. Human infection can result from ingestion of contaminated food or water from a variety of sources, including the consumption of fresh produce that is contaminated with the pathogen via the use of contaminated irrigation water. Using molecular methods, we investigated the occurrence of Campylobacter in the...
Mon, 03/21/2016 - 12:52
Abstract AimsTo investigate the erythromycin resistance patterns and mechanism for Campylobacter isolates in China. Methods and ResultsThe minimum inhibitory concentrations of erythromycin on 858 Chinese Campylobacter isolates were analysed. PCR and DNA sequencing were used to identify mutations in the 23S rRNA and the presence of the ermB gene in the 158 erythromycin resistance isolates (18.4...
Mon, 03/21/2016 - 12:03
Publication date: Available online 21 March 2016 Author(s): Natacha Laprade, Michel Cloutier, David R. Lapen, Edward Topp, Graham Wilkes, Richard Villemur, Izhar U.H. Khan Campylobacter species are one of the leading causes of bacterial gastroenteritis in humans worldwide. This twofold study was sought to: i) develop and optimize four single-tube multiplex PCR (mPCR) assays...
Mon, 03/21/2016 - 02:26
Abstract To determine if Campylobacter isolation method influenced antimicrobial susceptibility results, the minimum inhibitory concentrations (MIC) of nine antimicrobials were compared for 291 pairs of Campylobacter isolates recovered from chicken carcass rinse samples using direct plating and an enrichment method. Among the isolates 64.1% were C. jejuni, 35.7% were C. coli, and 0.2% were C....
Sun, 03/20/2016 - 18:03
Publication date: Available online 20 March 2016 Author(s): Sara Kovanen, Rauni Kivistö, Ann-Katrin Llarena, Ji Zhang, Ulla-Maija Kärkkäinen, Tamara Tuuminen, Jaakko Uksila, Marjaana Hakkinen, Mirko Rossi, Marja-Liisa Hänninen Campylobacter jejuni is the leading cause of bacterial gastroenteritis and chicken is considered a major reservoir and source of human...
Fri, 03/18/2016 - 18:01
Publication date: Available online 18 March 2016 Author(s): Tomasz Seliwiorstow, Julie Baré, Dirk Berkvens, Inge Van Damme, Mieke Uyttendaele, Lieven De Zutter Campylobacter carcass contamination was quantified across the slaughter line during processing of Campylobacter positive batches. These quantitative data were combined together with information describing slaughterhouse...
Fri, 03/18/2016 - 11:50
Publication date: Available online 18 March 2016 Author(s): Alexandra Duarte, Tomasz Seliwiorstow, William G. Miller, Lieven De Zutter, Mieke Uyttendaele, Katelijne Dierick, Nadine Botteldoorn The aim of this study was to compare different typing methods, individually and combined, for use in the monitoring of Campylobacter in food. C. jejuni (n=94) and C. coli (n=52) isolated...
Fri, 03/04/2016 - 23:01
While horizontal transmission is a route clearly linked to the spread of Campylobacter at the farm level, few studies support the transmission of Campylobacter spp. from breeder flocks to their offspring. Thus, the present study was carried out to investigate the possibility of vertical transmission. Breeders were monitored from the time of housing day-old chicks, then throughout the laying...
Sun, 02/28/2016 - 13:29
Publication date: Available online 27 February 2016 Author(s): Ratthaphol Charlermroj, Manlika Makornwattana, Irene R. Grant, Christopher T. Elliott, Nitsara Karoonuthaisiri This study rigorously evaluated a previously developed immunobead array method to simultaneously detect three important foodborne pathogens, Campylobacter jejuni, Listeria monocytogenes, and Salmonella spp.,...
Thu, 02/25/2016 - 17:31
Poultry processing plants in the United States were surveyed on their current Campylobacter and Salmonella control practices. Following surveys, data were collected to develop a baseline for prevalence rates of Salmonella and Campylobacter; then changes in practices were implemented and evaluated for improvements in pathogen control. Surveys were sent to the plant Quality Assurance managers to...
Thu, 02/25/2016 - 17:31
Poultry meat is the major source of human campylobacteriosis, the most frequently reported zoonosis in the EU. The prevalence of Campylobacter colonization in European broiler flocks is 71%. Despite considerable efforts, there is still no effective strategy available to prevent or reduce Campylobacter colonization in broilers. This study tested a wide variety of feed additives to reduce...
Fri, 02/19/2016 - 11:01
The gut microbiota plays important roles in its host. However, how each microbiota member contributes to the behavior of the whole population is not known. In this study, we therefore determined protein expression in the cecal microbiota in chickens of selected ages and in 7-day-old chickens inoculated with different cecal extracts on the day of hatching. Campylobacter, Helicobacter,...
Fri, 02/19/2016 - 11:01
Pathogen identification and microbial source tracking (MST) to identify sources of fecal pollution improve evaluation of water quality. They contribute to improved assessment of human health risks and remediation of pollution sources. An MST microarray was used to simultaneously detect genes for multiple pathogens and indicators of fecal pollution in freshwater, marine water, sewage-contaminated...
Fri, 02/19/2016 - 08:59
Abstract AimsUsing Bayesian methods that do not require the definition of a gold standard, the diagnostic sensitivity and specificity of a real-time polymerase chain reaction (PCR) assay are compared to those of an enriched culture assay for detection of Campylobacter in enriched comminuted chicken samples. Methods and ResultsFood Safety and Inspection Service comminuted chicken samples were...
Wed, 02/10/2016 - 19:00
Clinical isolates of Campylobacter spp. and Salmonella spp. are notifiable in Switzerland. In 1995, Campylobacter replaced Salmonella as the most frequently reported food-borne pathogen. We analysed notification data (1988-2013) for these two bacterial, gastrointestinal pathogens of public health importance in Switzerland. Notification rates were calculated using data for the average resident...
Fri, 02/05/2016 - 04:09
Publication date: Available online 4 February 2016 Author(s): Judit K. Kovács, Györgyi Horváth, Monika Kerényi, Béla Kocsis, Levente Emődy, György Schneider Direct bioautography is a useful method to identify antimicrobial compounds with potential therapeutic importance. Because of technical limitations till now, it has been applied only for aerobic bacteria. In this work we...
Mon, 02/01/2016 - 17:00
Mateusz Rawski, Bartosz Kierończyk, Jakub Długosz, Sylwester Świątkiewicz, Damian Józefiak Probiotics are widely used in nutrition, and their mode of action is intensively studied in mammals and birds; however, it is almost unknown in reptiles. In the present study, Trachemys scripta scripta and Sternotherus odoratus were used to assess the effects of dietary probiotics on chelonian...
Thu, 01/28/2016 - 09:46
Publication date: Available online 28 January 2016 Author(s): Jung-Whan Chon, Young-Ji Kim, Hong-Seok Kim, Dong-Hyeon Kim, Kwang-Young Song, Kidon Sung, Kun-Ho Seo Although cefoperazone is the most commonly used antibiotic in Campylobacter-selective media, the distribution of cefoperazone-resistant bacteria such as extended-spectrum beta-lactamase (ESBL)-producing Escherichia...
Sat, 01/23/2016 - 09:28
Publication date: 16 March 2016 , Volume 221 Author(s): Catherine M. Burgess, Andrea Gianotti, Nadia Gruzdev, John Holah, Susanne Knøchel, Angelika Lehner, Edyta Margas, Stephan Schmitz Esser, Shlomo Sela (Saldinger), Odile Tresse In combination with other strategies, hyperosmolarity and desiccation are frequently used by the food processing industry as a means to prevent...
Wed, 01/20/2016 - 10:31
Abstract AimThe aim of this study was to apply the most sensitive molecular techniques in combination with culture based methods to characterise broiler farms in terms of the timeline (‘appearance’ and ‘pattern’) of Campylobacter contamination prior and post detection in the birds. Methods and ResultsFaecal and environmental samples were collected from 3 broiler farms (2 flocks per farm). RT-PCR...
Tue, 01/19/2016 - 09:12
Publication date: 18 January 2016 , Volume 217 Author(s): Augustine Mpofu, Anita R. Linnemann, Martinus J.R. Nout, Marcel H. Zwietering, Eddy J. Smid, Heidy M.W. den Besten Mutandabota is a dairy product consumed as a major source of proteins and micronutrients in Southern Africa. In this study the microbial safety of traditional and a variant of mutandabota fermented with the...
Tue, 01/19/2016 - 09:12
Publication date: 2 March 2016 , Volume 220 Author(s): Leonard Koolman, Paul Whyte, Catherine Burgess, Declan Bolton Studies were undertaken to investigate the effect of oxidative stress conditions (exposure to hydrogen peroxide, H2O2) on [1] the expression of 14 Campylobacter jejuni virulence-associated genes associated with motility and/or invasion (flaA, flaB, flhA, flhB, ciaB,...

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