Campylobacter

Mon, 01/11/2016 - 06:52
Summary Backyard poultry has become increasingly popular in industrialized countries. In addition to keeping chickens for eggs and meat, owners often treat the birds as pets. However, several pathogenic enteric bacteria have the potential for zoonotic transmission from poultry to humans but very little is known about the occurrence of zoonotic pathogens in backyard flocks. The occurrence and the...
Fri, 01/08/2016 - 16:02
Abstract Polylactide (PLA) is the most mature biobased and biodegradable polymer. Due to its inherent brittleness, the polymer cannot be used as a packaging material without plasticizer. An attempt was made to develop antimicrobial plasticized PLA film by incorporating polyethylene glycol (PEG) and 3 essential oils (EO), namely cinnamon, garlic, and clove by solvent casting method. Physical,...
Thu, 01/07/2016 - 11:01
The objectives of this study were to identify antimicrobial resistance genotypes for Campylobacter and to evaluate the correlation between resistance phenotypes and genotypes using in vitro antimicrobial susceptibility testing and whole-genome sequencing (WGS). A total of 114 Campylobacter species isolates (82 C. coli and 32 C. jejuni) obtained from 2000 to 2013 from humans, retail meats, and...
Fri, 12/18/2015 - 08:28
Summary Although pets provide physiological and psychological benefits to their owners, they are a potential source of zoonotic infections, especially for vulnerable individuals such as immunocompromised patients. During 1 year, we therefore performed a pilot project, which included 32 immunocompromised Chilean children and their family pets (35 dogs and 9 cats) with the aim of detecting,...
Sat, 12/12/2015 - 03:43
Summary Syto9 and probe-based multiplex real-time PCR assays for simultaneous detection of a group of foodborne pathogens (named SYLC group), targeting Salmonella spp. (invA gene), Yersinia enterocolitica (ystA gene), Listeria monocytogenes (hly gene) and Campylobacter spp. (rrna gene), have been developed. The Syto9 assay generates amplicon DNA melting curve with four peaks of 86.5 ± 0.5, 84 ± 0...
Sat, 11/28/2015 - 19:08
Publication date: December 2015 , Volume 119 Author(s): Inés de Cárdenas, José F. Fernández-Garayzábal, María-Luisa de la Cruz, Lucas Domínguez, María Ugarte-Ruiz, Susana Gómez-Barrero This study evaluates an improved scheme for Campylobacter genotyping based on the combination of true and questionable CRISPR (clustered regularly interspaced short palindromic repeats) elements. A...
Wed, 11/25/2015 - 06:54
Summary Campylobacter jejuni is a causative pathogen of human acute bacterial gastroenteritis. Infected poultry products are regarded as a major source for human C. jejuni infection. The flagellar capping protein (FliD) is highly conserved among C. jejuni strains/isolates and is antigenic as analysed by immunoblot. In this study, we used the FliD protein as a probe to survey the prevalence of C....
Tue, 11/24/2015 - 09:29
Abstract AimsTo develop a rapid test for thermotolerant Campylobacter in poultry faeces. Methods and ResultsThe reported method is based on immunomagnetic separation and loop-mediated isothermal DNA amplification (IMS/LAMP). This LAMP assay is specific (demonstrated using 10 Campylobacter strains and 13 non-Campylobacter bacterial species) and sensitive (95 % probability of detecting 22 genome...
Mon, 11/23/2015 - 18:57
Publication date: Available online 11 November 2015 Author(s): Elodie Sifré, Salha Ben Amor, Astrid Ducournau, Pauline Floch, Hubert Chardon, Francis Mégraud, Philippe Lehours Antimicrobial susceptibility testing of Campylobacter isolates is of great importance for treatment options especially in systemic diseases. The European Committee for Antimicrobial and Susceptibility...
Fri, 11/20/2015 - 15:08
The ability of malic acid to reduce Campylobacter jejuni on chicken legs was evaluated. Chicken legs were inoculated with C. jejuni. After dipping legs either in water (control), 1 % or 2% malic acid solution (v/v), they were stored at 4C for 8 days. Changes in C. jejuni, psychrotrophs, Pseudomonas counts and sensorial characteristics (odor, color and overall appearance) were determined after...
Sat, 11/14/2015 - 00:56
Current regulatory guidelines to control poultry-borne pathogens, more specifically Campylobacter jejuni, necessitate the need for novel applications of antimicrobials during poultry processing. The objective of this study was to evaluate and compare the efficacy of peracetic acid (PAA; 100 and 200 ppm) and sodium hypochlorite (SH; 25 and 50 ppm) as antimicrobial interventions to reduce C. jejuni...
Fri, 11/13/2015 - 16:35
Abstract In this study, we investigated the distribution, phenotypic and molecular typing characters of Campylobacter jejuni in domestic fowl, and livestock populations in East China, to provide some reference for researches on its molecular epidemiology. A total of 1250 samples were collected from different animal sources, and C. jejuni strains were then isolated and tested for antibiotic...
Fri, 11/06/2015 - 02:11
Abstract Campylobacteriosis is the most prevalent bacterial foodborne gastroenteritis affecting humans in the European Union, and ranks second in the United States only behind salmonellosis. In Europe, there are about nine million cases of campylobacteriosis every year, making the disease a major public health issue. Human cases are mainly caused by the zoonotic pathogen Campylobacter jejuni. The...
Wed, 11/04/2015 - 09:16
Abstract AimsThis study investigated waterborne opportunistic pathogens (OPs) including potential hosts, and evaluated the use of Legionella spp. for indicating microbial water quality for OPs within a full-scale operating drinking water distribution system (DWDS). Methods and ResultsTo investigate the occurrence of specific microbial pathogens within a major city DWDS we examined large volume (...
Sat, 10/24/2015 - 02:27
The popularity of food produced from animals kept under an organic regimen has increased in recent years. In Germany, turkey meat consumption has increased. Despite several studies assessing the susceptibility of campylobacters to various antibiotics in poultry, no sufficient data exists regarding the antimicrobial resistance of campylobacters in organic-reared turkeys. This study provides...
Fri, 10/16/2015 - 05:11
Summary Campylobacter jejuni, the leading cause of human bacterial gastroenteritis, requires low environmental oxygen and high carbon dioxide for optimum growth, but the molecular basis for the carbon dioxide requirement is unclear. One factor may be inefficient conversion of gaseous CO2 to bicarbonate, the required substrate of various carboxylases. Two putative carbonic anhydrases (CA's) are...
Wed, 10/14/2015 - 20:00
In 1991, 1999 and 2006, randomly selected individuals from the Danish Central Personal Register provided a serum sample. From individuals aged 30 years and above, 500 samples from each year were analysed for Campylobacter IgG, IgA and IgM antibodies using a direct ELISA method. We applied a seroincidence calculator available from the European Centre for Disease Prevention and Control to perform...
Fri, 10/09/2015 - 04:11
Publication date: March 2016 , Volume 61 Author(s): Andreia Duarte, Ângelo Luís, Mónica Oleastro, Fernanda C. Domingues Foodborne diseases remain common around the world with Campylobacteriosis being the most commonly reported zoonosis in the European Union in 2013. Campylobacter jejuni and Campylobacter coli are the main species associated with human illness. Furthermore,...
Thu, 10/01/2015 - 03:20
Volume 61, Issue 10, Page 701-721, October 2015. Campylobacter species, particularly thermophilic campylobacters, have emerged as a leading cause of human foodborne gastroenteritis worldwide, with Campylobacter jejuni, Campylobacter coli, and Campylobacter lari responsible for the majority of human infections. Although most cases of campylobacteriosis are self-limiting, campylobacteriosis...
Tue, 09/29/2015 - 01:23
Flagellation in polar flagellates is one of the rare biosynthetic processes known to be numerically regulated in bacteria. Polar flagellates must spatially and numerically regulate flagellar biogenesis to create flagellation patterns for each species that are ideal for motility. FlhG ATPases numerically regulate polar flagellar biogenesis, yet FlhG orthologs are diverse in motif composition. We...
Mon, 09/28/2015 - 03:29
Canada geese (Branta canadensis) faeces have been shown to contain pathogenic protozoa and bacteria in numerous studies over the past 15 years. Further, increases in both the Canada geese populations and their ideal habitat requirements in the United States (US) translate to a greater presence of these human pathogens in public areas, such as recreational freshwater beaches. Combining these...
Mon, 09/28/2015 - 01:16
Publication date: February 2016 , Volume 53, Part B Author(s): Ar’Quette Grant, Fawzy Hashem, Salina Parveen Salmonella and Campylobacter are major causes of foodborne related illness and are traditionally associated with consuming undercooked poultry and/or consuming products that have been cross contaminated with raw poultry. Many of the isolated Salmonella and Campylobacter that can...
Sat, 09/26/2015 - 01:20
A study was conducted to evaluate a high pressure spray (HPS) with water as an alternative to trimming to remove gastrointestinal contamination on poultry carcasses and improve microbiological quality. The study was conducted under commercial conditions in 5 slaughter plants with one plant presenting approximately 5% of carcasses with visible gastrointestinal contamination (VGC), and the others...
Sat, 09/26/2015 - 01:20
This experiment was conducted to evaluate the effects of feeding dietary fiber on cecal short-chain fatty acid (SCFA) concentration and cecal microbiota of broiler and laying-hen chicks. The lower fiber diet was based on corn-soybean meal (SBM) and the higher fiber diet was formulated using corn-SBM-dried distillers grains with solubles (DDGS) and wheat bran to contain 60.0 g/kg of both DDGS and...
Sat, 09/26/2015 - 01:20
This study investigated the hypothesis that dietary fats rich in lauric (C12) and myristic acid (C14) increase broiler performance and that the underlying mechanism involves antimicrobial effects on gut bacteria and changes in gut morphology. One hundred eighty 1-day-old Cobb-500 broilers were allotted to 3 groups. All groups received a basal diet consisting of maize, wheat, soybean meal, and a...
Fri, 09/25/2015 - 02:16
Within the European activities for the ‘Monitoring and Collection of Information on Zoonoses’, annually EFSA publishes a European report, including information related to the prevalence of Campylobacter spp. in Germany. Spatial epidemiology becomes here a fundamental tool for the generation of these reports, including the representation of prevalence as an essential element. Until now, choropleth...
Wed, 09/23/2015 - 03:20
Sensing potential nitrogen-containing respiratory substrates such as nitrate, nitrite, hydroxylamine, nitric oxide (NO) or nitrous oxide (N2O) in the environment and subsequent up-regulation of corresponding catabolic enzymes is essential for many microbial cells. The molecular mechanisms of such adaptive responses are, however, highly diverse in different species. Here, induction of periplasmic...
Wed, 09/23/2015 - 01:23
Banya Banowary, Van Tuan Dang, Subir Sarker, Joanne H. Connolly, Jeremy Chenu, Peter Groves, Michelle Ayton, Shane Raidal, Aruna Devi, Thiru Vanniasinkam, Seyed A. Ghorashi Campylobacter spp. are important causes of bacterial gastroenteritis in humans in developed countries. Among Campylobacter spp. Campylobacter jejuni (C. jejuni) and C. coli are the most common causes of human infection. In...
Thu, 09/17/2015 - 01:23
Multicellular biofilms are an ancient bacterial adaptation that offers a protective environment for survival in hostile habitats. In microaerophilic organisms like Campylobacter, biofilms play a key role in transmission to humans as the bacteria are exposed to atmospheric oxygen concentrations when leaving the reservoir host gut. Genetic determinants of biofilm formation differ between species,...
Sun, 09/06/2015 - 01:22
Publication date: 23 December 2015 , Volume 215 Author(s): Ewa Pacholewicz, Apostolos Liakopoulos, Arno Swart, Betty Gortemaker, Cindy Dierikx, Arie Havelaar, Heike Schmitt Whilst broilers are recognised as a reservoir of extended-spectrum-β-lactamase (ESBL)- and AmpC-β-lactamase (AmpC)-producing Escherichia coli , there is currently limited knowledge on the effect of...
Fri, 09/04/2015 - 01:23
Publication date: Available online 3 September 2015 Author(s): Chen Zhang, Peihua Niu, Yanying Hong, Ji Wang, Jingyun Zhang, Xuejun Ma Objectives We aim to develop a multiplex real-time PCR assay to detect the most common pathogens causing community outbreaks of diarrhea. Methods Four reaction systems of fluorescence dye-based real-time PCR assay were performed to amplify genes...
Tue, 08/25/2015 - 01:23
Campylobacter jejuni (C. jejuni) is a leading cause of human bacterial gastroenteritis worldwide. Previous research has shown that circadian rhythm plays a critical role in host response to C. jejuni colonization. The CLOCK gene is one of the core genes regulating circadian rhythms and shows significant expression on 7 d post-C. jejuni inoculation. The objective of this study was to investigate...
Tue, 08/25/2015 - 01:23
Marinades containing polyphosphates have been previously implicated in the enhanced survival of Campylobacter spp. in poultry product exudates. The enhanced Campylobacter survival has been attributed primarily to the ability of some polyphosphates to change the pH of the exudate to one more amenable to Campylobacter. In this study a ground poultry product contaminated with a 6 strain...
Sat, 08/22/2015 - 01:27
Aims The aim of this study was to cost-effectively improve detection of foodborne pathogens in PCR inhibitory samples through the use of alternative DNA polymerases. Methods and Results Commercially available polymerases (n=16) and PCR master mixes (n=4) were screened on DNA purified from bacterial cells in two validated real-time PCR assays for Campylobacter and Salmonella. The five best...
Tue, 08/18/2015 - 01:33
Campylobacter is one of the leading causes of foodborne illness worldwide and is associated with consumption of poultry products. One potential strategy to reduce Campylobacter colonization in poultry is the use of probiotics. In an attempt to reduce Campylobacter in poultry, we isolated 117 GRAS (generally recognized as safe) bacterial isolates from the ceca of healthy birds and evaluated their...
Tue, 08/18/2015 - 01:33
Campylobacter occur in fresh retail poultry products as a result of their colonization of the gastro-intestinal tract of chickens during growth. Feed additives could be used for suppression of Campylobacter levels in the chickens prior to slaughter. To address this opportunity, feed manufacturers are targeting natural antimicrobials from plant material as new forms of consumer-accepted feed...
Mon, 08/17/2015 - 01:24
Campylobacter have been found in lymphoid tissue of poultry, but the location in these tissues has not been determined. The objective of this study was to determine if Campylobacter are on the outside or on the inside of the spleen. For external (ES) spleen sampling 2 days post-inoculation, each spleen was rinsed with 3 mL of Bolton's enrichment broth (BEB). For internal (IS) spleen sampling,...
Wed, 08/12/2015 - 01:21
Campylobacters are a leading cause of gastrointestinal morbidity worldwide and the majority of human infections are triggered by eating foods contaminated with Campylobacter jejuni or Campylobacter coli. Campylobacters are equally notorious for their ability to mimic human glycoconjugate structures and for their capacity to synthesize both N- and O-linked glycoproteins. These species were once...
Wed, 08/12/2015 - 01:21
Thermophilic Campylobacter species colonize the intestine of agricultural and domestic animals commensally, but cause severe gastroenteritis in humans. In contrast to other enteropathogenic bacteria, Campylobacter have been considered to be non-glycolytic, a metabolic property originally used for their taxonomic classification. Contrary to this dogma, we demonstrate that several Campylobacter...
Mon, 08/10/2015 - 03:20
Four trials were carried out at a broiler processing plant to examine the effectiveness of spraying lactic acid solutions for reducing the numbers of Campylobacter on carcasses. The carcasses were naturally contaminated and treated after the inside–outside washer and before the air chiller. Carcasses were treated by spraying in a tunnel or with one of two hand-held sprayers. Carcasses were...
Sat, 08/08/2015 - 01:19
Publication date: 30 September 2015 , Volume 179, Issues 3–4 Author(s): Tim R. Crawshaw, Jeremy I. Chanter, Stuart C.L. Young, Shaun Cawthraw, Adrian M. Whatmore, Mark S. Koylass, Ana B. Vidal, Francisco J. Salguero, Richard M Irvine The condition known as spotty liver disease or spotty liver syndrome can cause significant mortality in free range laying hen flocks. It has...
Thu, 08/06/2015 - 01:22
Campylobacter jejuni, one of the most common causes of gastroenteritis worldwide, is transmitted to humans through poultry. We previously reported that Lactobacillus gasseri SBT2055 (LG2055) reduced C. jejuni infection in human epithelial cells in vitro and inhibited pathogen colonization of chickens in vivo. This suggested that the LG2055 adhesion and/or co-aggregation phenotype mediated by cell...
Mon, 08/03/2015 - 03:20
Trivalent organoarsenic compounds are far more toxic than either pentavalent organoarsenicals or inorganic arsenite. Many microbes methylate inorganic arsenite (As(III)) to more toxic and carcinogenic methylarsenite (MAs(III)). Additionally, monosodium methylarsenate (MSMA or MAs(V)) has been used widely as an herbicide and is reduced by microbial communities to MAs(III). Roxarsone (3-nitro-4-...
Sun, 08/02/2015 - 01:25
Publication date: December 2015 , Volume 52 Author(s): Sabrina Macé, Nabila Haddad, Monique Zagorec, Odile Tresse Campylobacter is the leading cause of bacterial enteritis in the world. For this reason, this pathogen is widely studied. As a microaerophilic and capnophilic microorganism, this foodborne pathogen requires an atmosphere with reduced oxygen (O 2 ) and elevated carbon...

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