Research Publications (Food Safety)

Molecular Microbiology, Accepted Article. Campylobacter jejuni is a microaerophilic zoonotic pathogen with an atypical respiratory Complex I that oxidises a flavodoxin (FldA) instead of NADH. FldA is essential for viability and is reduced via pyruvate and 2‐oxoglutarate oxidoreductases (POR/OOR). Here, we show that FldA can also be reduced by FqrB (Cj0559), an NADPH:flavodoxin reductase. An fqrB deletion mutant was viable but displayed a significant growth defect.

It has been well documented that cytolethal distending toxin (CDT) from Helicobacter hepaticus (H. hepaticus), Campylobacter jejuni (C. jejuni) and other Gram-negative intestinal pathogens is linked to the inflammatory bowel disease (IBD). However, the mechanisms underlying the progression of H. hepaticus induced colitis remains unclear. In this study, male B6.129P2-IL10tm1Cgn/J mice were infected by H. hepaticus and ΔCdtB H. hepaticus for 6, 12, 18, and 24 weeks. Histopathology, H.

by Brendha Truccollo, Paul Whyte, Catherine Burgess, Declan Bolton

Author(s): A.Arun Prince Milton, Kasanchi.M. Momin, G.Bhuvana Priya, Samir Das, M. Angappan, Arnab Sen, D.K. Sinha, Sandeep Ghatak

Campylobacter is the leading cause of human bacterial diarrhoeal disease worldwide, with poultry meat products accounting for the majority of human cases. Recent surveys by the Food Standards Agency estimate the Campylobacter prevalence in fresh UK retail chicken to be 41.2%.

Author(s): Juan Wang, Zhi Wang, Jumei Zhang, Yu Ding, Zonghao Ma, Fufeng Jiang, Xiang Nie, Shengjun Tang, Moutong Chen, Shi Wu, Haiyan Zeng, Tao Lei, Xiaojuan Yang, Shuhong Zhang, Qingping Wu

Our study aimed to determine the prevalence of Campylobacter jejuni isolated from raw milk, cheese, and human stool samples in Beni-Suef Governorate, Egypt, and to characterize the antibiotic resistance profile and virulence genes of the isolates. An additional objective was to evaluate the effectiveness of cinnamon oil and Lactobacillus acidophilus La5 for controlling C. jejuni in cheese.

Author(s): Natalie Rauber Kleinubing, Tassiana Ramires, Simone de Fátima Rauber Würfel, Louise Haubert, Leticia Klein Scheik, Frederico Schmitt Kremer, Graciela Volz Lopes, Wladimir Padilha da Silva

Campylobacter jejuni is an important foodborne pathogen causing campylobacteriosis. It can infect humans through the consumption of contaminated chicken products or via the direct handling of animals. Diallyl trisulfide (DATS) is a trisulfide compound from garlic extracts that has a potential antimicrobial effect on foodborne pathogens. This study investigated the antimicrobial activity of DATS on C. jejuni by evaluating the minimal inhibitory concentrations (MICs) of C.

The aim of the present study was to address method-dependent implications during the quantification of viable Campylobacter coli cells on meat over time. Traditional colony counting on selective and non-selective culture media along with an optimized viability real-time PCR utilizing propidium monoazide-quantitative PCR (PMA-qPCR), spheroplast formation and an internal sample process control (ISPC), were comparatively evaluated for monitoring the survival of C.

Author(s): Torrung Vetchapitak, Mohammad Shohel Rana, Satomi Sasaki, Takako Taniguchi, Susumu Sugiyama, Junichiro Soejima, Taradon Luangtongkum, Yuya Yamaguchi, Naoaki Misawa

by Mario A. Monteiro, Yu-Han Chen, Zuchao Ma, Cheryl P. Ewing, Nooraisyah Mohamad Nor, Eman Omari, Ellen Song, Pawel Gabryelski, Patricia Guerry, Frédéric Poly

Author(s): Ali Al-Sakkaf

The sustainable utilization of black soldier fly (BSF) for recycling organic waste into nutrient-rich biomass, such as high-quality protein additive, is gaining momentum, and its microbiota is thought to play important roles in these processes.

by Yohans Hagos, Getachew Gugsa, Nesibu Awol, Meselu Ahmed, Yisehak Tsegaye, Nigus Abebe, Abrha Bsrat

Campylobacter spp. are the leading global cause of bacterial colon infections in humans. Enteropathogens are subjected to several stress conditions in the host colon, food complexes, and the environment. Species of the genus Campylobacter, in collective interactions with certain enteropathogens, can manage and survive such stress conditions. The stress-adaptation mechanisms of Campylobacter spp.

Cyadox has potential use as an antimicrobial agent in animals. However, its pharmacodynamic properties have not been systematically studied yet. In this study, the in vitro antibacterial activities of cyadox were assayed, and the antibacterial efficacy of cyadox against facultative anaerobes was also determined under anaerobic conditions.

Campylobacter jejuni is the leading cause of bacterial foodborne gastroenteritis world wide and represents a major public health concern. Over the past two decades, significant progress in functional genomics, proteomics, enzymatic-based virulence profiling (EBVP), and the cellular biology of C. jejuni have improved our basic understanding of this important pathogen. We review key advances in our understanding of the multitude of emerging virulence factors that influence the outcome of C.

Campylobacter, the most common etiologic agent of zoonotic gastroenteritis in humans, is present in many reservoirs including livestock animals, wildlife, soil, and water. Previously, we reported a novel Campylobacter jejuni strain SCJK02 (MLST ST-8388) from the gut of wild mice (Micromys minutus) using culture-dependent methods. However, due to fastidious growth conditions and the presence of viable but non-culturable Campylobacter spp., it is unclear whether M.

Campylobacter contaminated poultry remains the major cause of foodborne gastroenteritis worldwide, calling for novel antibacterials. We previously developed the concept of Innolysin composed of an endolysin fused to a phage receptor binding protein (RBP) and provided the proof-of-concept that Innolysins exert bactericidal activity against Escherichia coli. Here, we have expanded the Innolysin concept to target Campylobacter jejuni. As no C.

Author(s): Azam Ali Sher, Muhammad Adnan Ashraf, Bahar-E Mustafa, Muhammad Mohsin Raza

Campylobacter species are Gram-negative, motile, and non–spore-forming bacteria with a unique helical shape that changes to filamentous or coccoid as an adaptive response to environmental stresses. The relatively small genome (1.6 Mbp) of Campylobacter with unique cellular and molecular physiology is only understood to a limited extent.

by Delfina F. Hlashwayo, Betuel Sigaúque, Emília V. Noormahomed, Sónia M. S. Afonso, Inácio M. Mandomando, Custódio G. Bila

Introduction

Author(s): Arturo B. Soro, Paul Whyte, Declan J. Bolton, Brijesh K. Tiwari

Author(s): Michael S. Williams, Eric D. Ebel, Epiphanie Nyirabahizi