Campylobacter

Fri, 06/05/2015 - 01:24
Publication date: Available online 4 June 2015 Author(s): S.U. Wetzels , E. Mann , B.U. Metzler-Zebeli , M. Wagner , F. Klevenhusen , Q. Zebeli , S. Schmitz-Esser Ecological balance in the rumen is highly sensitive to concentrate-rich diets. Yet the effects of these feeding practices on the caprine bacterial epimural microbiome (CBEM), a microbial community with putative important...
Fri, 06/05/2015 - 01:24
Campylobacter jejuni, the leading bacterial cause of human gastroenteritis in the United States, displays significant strain diversity due to horizontal gene transfer. Conjugation is an important horizontal gene transfer mechanism contributing to the evolution of bacterial pathogenesis and antimicrobial resistance. It has been observed that heat shock could increase transformation efficiency in...
Thu, 05/28/2015 - 01:27
An observational study examining prevalence of Campylobacter and Salmonella in environmental samples and biosecurity interventions to mitigate these 2 pathogens was performed for 3 consecutive flocks on a commercial broiler farm in northeast Georgia. Monitoring was performed first on a baseline flock for which no interventions or treatments were undertaken. The same monitoring was performed for 2...
Thu, 05/28/2015 - 01:27
In-house litter composting has been reintroduced to the industry and shown to reduce bacteria by as much as 2 orders of magnitude. Other industries have demonstrated that pathogens can recolonize a waste-residual when microbial competition has been reduced or inhibited following composting. Poultry growers, in the process of shifting to in-house composting for pathogen control, should be aware of...
Thu, 05/28/2015 - 01:27
Campylobacter is a natural member of the gut microflora in many commercial broilers and as such can become a contaminant on edible surfaces during processing. Culturing gut contents or feces can be a means to determine flock status prior to live-haul. The wide variety of non-Campylobacter background bacteria in these complex samples contaminates growth media and can make it very difficult to...
Sat, 05/23/2015 - 01:21
Publication date: 3 August 2015 , Volume 206 Author(s): Evy Lahou , Xiang Wang , Elien De Boeck , Elien Verguldt , Annemie Geeraerd , Frank Devlieghere , Mieke Uyttendaele In order to evaluate the effect of simulated home pan frying of raw meat and meat preparations of different animal species on the thermal inactivation of pathogens, the heat resistance (D-value) of three strains of...
Sun, 05/17/2015 - 03:20
Publication date: 16 July 2015 , Volume 205 Author(s): Ewa Pacholewicz , Arno Swart , Maarten Schipper , Betty G.M. Gortemaker , Jaap A. Wagenaar , Arie H. Havelaar , Len J.A. Lipman The causes of differences in Campylobacter and Escherichia coli concentrations on broiler chicken carcasses after chilling between slaughterhouses are not fully identified. Therefore, it is a challenge for...
Fri, 05/15/2015 - 03:20
We report a case of Campylobacter volucris bacteremia in an immunocompromised patient with polycythemia vera and alcoholic liver cirrhosis. To our knowledge, this is the first case report in which this organism has been isolated from a human clinical specimen.
Wed, 05/06/2015 - 01:16
Publication date: Available online 4 May 2015 Author(s): Petra Griekspoor , Eva Olsson Engvall , Britt Åkerlind , Björn Olsen , Jonas Waldenström Campylobacter jejuni is an important food-borne pathogen, with a global distribution. It can colonize numerous host species, including both domestic and wild animals, but is particularly associated with birds (poultry and wild birds). For...
Wed, 05/06/2015 - 01:16
Campylobacter jejuni is a highly diverse species of bacteria commonly associated with infectious intestinal disease of humans and zoonotic carriage in poultry, cattle, pigs, and other animals. The species contains a large number of distinct clonal complexes that vary from host generalist lineages commonly found in poultry, livestock, and human disease cases to host-adapted specialized lineages...
Mon, 05/04/2015 - 01:22
Publication date: September 2015 , Volume 50 Author(s): Igor Gruntar , Majda Biasizzo , Darja Kušar , Mateja Pate , Matjaž Ocepek Six slaughter batches deriving from six typical industrial broiler flocks were examined for the presence, quantity and genetic characteristics of contaminating Campylobacter jejuni ( C. jejuni ) during various stages of slaughtering and carcass processing. To...
Mon, 05/04/2015 - 01:22
Publication date: Available online 1 May 2015 Author(s): Mathilde H. Josefsen , Sandra C. Andersen , Julia Christensen , Jeffrey Hoorfar The efficiency of ten widely applied DNA extraction protocols was evaluated for suitability for diagnostic metagenomics. The protocols were selected based on a thorough literature study. Chicken fecal samples inoculated with about 1 × 103 and 1 × 106...
Mon, 05/04/2015 - 01:22
Aims In the present study, we conducted a quantitative microbial risk assessment forecasting the exposure to Campylobacter jejuni contaminated surfaces during preparation of chicken fillets and how using a disinfectant wipe intervention to clean a contaminated work area decreases the risk of infection following the preparation of raw chicken fillet in a domestic kitchen. Methods and Results...
Thu, 04/23/2015 - 01:22
Understanding of the colonization process of epithelial bacteria attached to the rumen tissue during rumen development is very limited. Ruminal epithelial bacterial colonization is of great significance for the relationship between the microbiota and the host and can influence the early development and health of the host. MiSeq sequencing of 16S rRNA genes and quantitative real-time PCR (qPCR)...
Sat, 04/18/2015 - 01:21
Aim To examine the occurrence and quantity of potential pathogens and an indicator of microbial contamination in the sediments of municipal drinking water storage tanks (MDWSTs), given the absence of such data across the United States. Methods and Results Sediment samples (87 MDWST) from eighteen locations across ten states of the U.S. were collected and assayed by qPCR for a range of potential...
Fri, 04/17/2015 - 03:20
Diarrhea due to enteric bacterial pathogens causes significant morbidity and mortality in the United States and worldwide. However, bacterial pathogens may be infrequently identified. Currently, culture and enzyme immunoassays (EIAs) are the primary methods used by clinical laboratories to detect enteric bacterial pathogens. We conducted a multicenter evaluation of the BD Max enteric bacterial...
Tue, 04/07/2015 - 01:28
Publication date: October 2015 , Volume 56 Author(s): Anthony Pavic , Julian M. Cox , Jeremy W. Chenu In Australia, increased consumer demand for chicken meat and socioeconomic pressures have forced poultry operations to extend plant operating time with delayed spin-chiller emptying and cleaning to maximise water use efficiency. There are concerns that these measures may compromise the...
Thu, 04/02/2015 - 01:21
The microaerophilic food-borne pathogen Campylobacter jejuni uses complex cytochrome-rich respiratory chains for growth and host colonization. Cytochrome c biogenesis requires haem ligation to reduced apocytochrome cysteines, catalysed by the cytochrome c synthase, CcsBA. While ccsBA could not be deleted, we showed that the thiol reductase DsbD and the CcsX homologue Cj1207 are involved in, but...
Fri, 01/30/2015 - 01:18
We encountered a rare case of severe fatal infection in a 70-year-old woman due to Campylobacter upsaliensis, identified by PCR amplification and sequencing analysis of the 16S rRNA gene using DNA extracted from the isolates. To our knowledge, fatal sepsis due to this organism has never been described to date.
Fri, 01/30/2015 - 01:18
An unusual increase in the number of Campylobacter concisus isolates found in stool cultures provoked an outbreak investigation at Bern University Hospital. No epidemiological links were found between the cases, and the Campylobacter isolates were clonally unrelated. A change in culture conditions to a hydrogen-rich atmosphere enhancing growth of C. concisus was deemed responsible for this pseudo...
Fri, 01/30/2015 - 00:08
The antimicrobial effect of spice-based marinades against Campylobacter jejuni on inoculated fresh broiler wings was investigatedExperiments were carried out with 1 strain of C. jejuni and 6 marinades. Four experimental marinades were composed for the study and contained spices (thyme, rosemary, basil, marjoram, and so on) and different combination of bioactive compounds. Two marinades were...
Wed, 01/21/2015 - 01:16
Publication date: June 2015 , Volume 48 Author(s): Lesley L. Duffy , Patrick J. Blackall , Rowland N. Cobbold , Narelle Fegan Poultry are considered a major source for campylobacteriosis in humans. A total of 1866 Campylobacter spp. isolates collected through the poultry processing chain were typed using flaA -restriction fragment length polymorphism to measure the impact of processing on...
Wed, 01/21/2015 - 01:16
Publication date: May 2015 , Volume 47 Author(s): Yiping He , Sue Reed , Arun K. Bhunia , Andrew Gehring , Ly-Huong Nguyen , Peter L. Irwin Campylobacter jejuni and Campylobacter coli are the two important species responsible for most of the Campylobacter infections in humans. Reliable isolation and detection of Campylobacter spp. from food samples are challenging due to the...
Wed, 01/21/2015 - 01:16
Publication date: June 2015 , Volume 48 Author(s): Declan J. Bolton Despite over 30 years of research, campylobacteriosis is the most prevalent foodborne bacterial infection in many countries including in the European Union and the United States of America. However, relatively little is known about the virulence factors in Campylobacter or how an apparently fragile organism can survive in...
Wed, 01/21/2015 - 01:16
Publication date: 2 March 2015 , Volume 196 Author(s): Marie-Josée J. Mangen , Martijn Bouwknegt , Ingrid H.M. Friesema , Juanita A. Haagsma , Laetitia M. Kortbeek , Luqman Tariq , Margaret Wilson , Wilfrid van Pelt , Arie H. Havelaar To inform risk management decisions on control and prevention of food-related disease, both the disease burden expressed in Disability Adjusted Life...
Wed, 01/21/2015 - 01:16
Aim To determine the presence of the T6SS in Campylobacter jejuni from diverse sources. Methods and Results The recently identified type VI secretion system (T6SS) is a bacterial injection machinery that plays a role in virulence, symbiosis, bacterial interactions and environmental stress responses. This system has been recently discovered in the major enteric pathogen Campylobacter jejuni. In...
Wed, 01/21/2015 - 01:16
Catalase enzymes detoxify H2O2 by the dismutation of H2O2 into O2 and H2O through the use of hemin cofactors. While the structure and biochemical properties of catalase enzymes have been well characterized over many decades of research, it remained unclear how catalases acquire hemin. We have previously reported that Cj1386 is essential for ensuring proper hemin content in Campylobacter jejuni...
Wed, 01/21/2015 - 01:16
Campylobacter jejuni is a leading cause of food-borne gastroenteritis in humans. It lives commensally in the gastrointestinal tract of animals, and tolerates variable conditions during transit/colonization of susceptible hosts. The C. jejuni CprRS two-component system contains an essential response regulator (CprR), and deletion of the cprS sensor kinase enhances biofilms. We sought to identify...
Wed, 01/21/2015 - 01:16
Yannick Rossez, Eliza B. Wolfson, Ashleigh Holmes, David L. Gally, Nicola J. Holden The flagellum organelle is an intricate multiprotein assembly best known for its rotational propulsion of bacteria. However, recent studies have expanded our knowledge of other functions in pathogenic contexts, particularly adherence and immune modulation, e.g., for Salmonella enterica, Campylobacter jejuni,...

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