Campylobacter

Tue, 05/12/2020 - 03:43
Author(s): Akram R. Alaboudi, Ismail M. Malkawi, Tareq M. Osaili, Ehab A. Abu-Basha, Javier Guitian Campylobacter is the world's leading cause of bacterial gastroenteritis, causing nearly 9 million cases of food poisoning in Europe every year. Poultry is considered the main source of Campylobacter infection to humans. The objectives of the study were to determine occurrence of C. jejuni and C....
Fri, 05/08/2020 - 03:48
Author(s): Matthew J. Sylte, Daniel C. Shippy, Bradley L. Bearson, Shawn M.D. Bearson Consumption of contaminated poultry products, including chicken livers, is the main source of human campylobacteriosis and approximately 90% of human cases are caused by Campylobacter jejuni subsp. jejuni (C. jejuni). Recent culinary trends that favor undercooked chicken livers may be responsible for outbreaks....
Fri, 05/08/2020 - 03:40
Aiming at investigating the potential sources of Campylobacter spp. contamination in pig slaughterhouse in eastern China, a total of 2056 samples were collected in pork production chain stretching from the upstream farm to the slaughterhouse, including 54 cloacal swabs from 54 live pigs on farm, 1726 samples from 214 pig carcasses along the eight main steps in the slaughtering line, and 276...
Wed, 05/06/2020 - 03:45
The present study investigated the effects of combined ultrasound (37 kHz, 380 W for 5 min) and peroxyacetic acid (PAA; 50–200 ppm) treatment on the reduction of Salmonella Typhimurium and Campylobacter jejuni on chicken skin. Ultrasound was not sufficient to inactivate S. Typhimurium (0.48 log CFU/g reduction) or C. jejuni (0.25 log CFU/g reduction), whereas PAA significantly (p < .05)...
Tue, 05/05/2020 - 03:43
Background Campylobacter bacteria infect both humans and animals. Sources of human exposure include contaminated food and water, contact with animals and/or their faeces, and contact with infected individuals. The objectives of this study were to: (a) identify environmental conditions associated with the occurrence of Campylobacter in humans in four regions of Ontario, and (b) identify pooled...
Mon, 05/04/2020 - 03:38
Campylobacteriosis is one of the most common bacterial infections worldwide causing economic costs. The high prevalence of Campylobacter spp. in poultry meat is a result of several contamination and cross‐contamination sources through the production chain. Moreover, survival mechanisms, such as biofilm formation, viable but nonculturable state, and antimicrobial resistance, enable its persistence...
Fri, 05/01/2020 - 03:46
Campylobacter species are the leading cause of gastroenteritis worldwide and an emerging threat in developing countries. Here, we report the draft whole-genome sequences of 51 Campylobacter jejuni and 12 Campylobacter coli strains isolated from patients with gastroenteritis in Santiago, Chile.
Wed, 04/29/2020 - 03:46
ABSTRACT Bacterial flagella are rotating nanomachines required for motility. Flagellar gene expression and protein secretion are coordinated for efficient flagellar biogenesis. Polar flagellates, unlike peritrichous bacteria, commonly order flagellar rod and hook gene transcription as a separate step after production of the MS ring, C ring, and flagellar type III secretion system (fT3SS) core...
Tue, 04/28/2020 - 03:35
This study describes the development and use of bacteriophage cocktails to control Campylobacter in broiler chickens, in a commercial setting, in Queensland Australia, following the birds from farm to the processing plant. The components of the bacteriophage cocktails were selected to be effective against the maximum number of Campylobacter jejuni and Campylobacter coli isolates encountered on SE...
Wed, 04/22/2020 - 03:44
by Anniina Jaakkonen, Rauni Kivistö, Maria Aarnio, Jenni Kalekivi, Marjaana Hakkinen Campylobacter jejuni has caused several campylobacteriosis outbreaks via raw milk consumption. This study reports follow-up of a milk-borne campylobacteriosis outbreak that revealed persistent C. jejuni contamination of bulk tank milk for seven months or longer. Only the outbreak-causing strain, representing...
Sun, 04/19/2020 - 03:46
Campylobacter spp. have been recognized as major foodborne pathogens worldwide. An increasing frequency of antibiotic-resistant pathogens, including Campylobacter spp., have been identified to transmit from food products to humans and cause severe threats to public health. To better mitigate the antibiotic resistance crisis, rapid detection methods are required to provide timely antimicrobial...
Wed, 04/15/2020 - 03:25
Sequencing studies have shown that optimal vaginal microbiota (VMB) are lactobacilli-dominated and that anaerobes associated with bacterial vaginosis (BV-anaerobes) are commonly present. However, they overlooked a less prevalent but more pathogenic group of vaginal bacteria: the pathobionts that cause maternal and neonatal infections and pelvic inflammatory disease. We conducted an individual...
Tue, 04/14/2020 - 03:33
LWT
Author(s): Ye Ji Song, Hwan Hee Yu, Yeon Jin Kim, Na-Kyoung Lee, Hyun-Dong Paik Papain is a cysteine protease isolated from Carica papaya that has been used for various purpose in the food industry. The aim of this study was to investigate the effects of papain on Staphylococcus aureus (S. aureus) and Campylobacter jejuni (C. jejuni) biofilms. Papain did not affect the viability of cells, even at...
Sat, 04/11/2020 - 03:26
Campylobacter jejuni (C. jejuni) is one of the most common bacteria responsible for human gastroenteritis worldwide. The mode of human transmission is foodborne infections due to consumption of contaminated food,...
Fri, 04/10/2020 - 03:29
Campylobacter jejuni, a foodborne pathogen, is one of the most common bacterial causes of gastroenteritis in the world. Undercooked poultry, raw (unpasteurized) dairy products, untreated water, and contaminated produce are the most common sources associated with infection. C. jejuni establishes a niche in the gut by adhering to and invading epithelial cells, which results in diarrhea with blood...
Thu, 04/09/2020 - 03:30
Microbiological surveillance of the food chain plays a critical role in improving our understanding of the distribution and circulation of food-borne pathogens along the farm to fork continuum toward the development of interventions to reduce the burden of illness. The application of molecular subtyping to bacterial isolates collected through surveillance has led to the identification of strains...
Thu, 04/02/2020 - 03:33
In a structured survey of all major chicken-meat producers in Australia, we investigated the antimicrobial resistance (AMR) and genomic characteristics of Campylobacter jejuni (n = 108) and C. coli (n = 96) from cecal samples of chickens at slaughter (n = 200). The majority of the C. jejuni (63%) and C. coli (86.5%) samples were susceptible to all antimicrobials. Fluoroquinolone resistance was...
Fri, 03/27/2020 - 03:42
Campylobacter spp. are one of the most important food-borne pathogens, which are quite susceptible to environmental or technological stressors compared to other zoonotic bacteria. This might be due to the lack of many stress response mechanisms described in other bacteria. Nevertheless, Campylobacter is able to survive in the environment and food products. Although some aspects of the heat stress...
Tue, 03/24/2020 - 04:04
by Anna Marta Banaś, Katarzyna Marta Bocian-Ostrzycka, Maciej Plichta, Stanisław Dunin-Horkawicz, Jan Ludwiczak, Jagoda Płaczkiewicz, Elżbieta Katarzyna Jagusztyn-Krynicka Posttranslational generation of disulfide bonds catalyzed by bacterial Dsb (disulfide bond) enzymes is essential for the oxidative folding of many proteins. Although we now have a good understanding of the Escherichia coli...
Tue, 03/24/2020 - 03:43
FlhF is a key protein required for complete flagellar synthesis, and its deletion results in the complete absence of a flagella and thus motility in Campylobacter jejuni. However, the specific mechanism still remains unknown. In this study, RNA-Seq, EMSAs, ChIP-qPCR and β-Galactosidase assays were performed to elucidate the novel interactions between FlhF and genes involved in flagellar synthesis...
Sat, 03/21/2020 - 03:26
Many bacterial pathogens display glycosylated surface structures that contribute to virulence, and targeting these structures is a viable strategy for pathogen control. The foodborne pathogen Campylobacter jejuni expresses a vast diversity of flagellar glycans, and flagellar glycosylation is essential for its virulence. Little is known about why C. jejuni encodes such a diverse set of flagellar...
Thu, 03/19/2020 - 03:33
Campylobacteriosis is the leading foodborne bacterial diarrheal illness in many countries, with up to 80% of human cases attributed to the avian reservoir. The only control strategies currently available are stringent on-farm biosecurity and carcass treatments. Heritable differences in the resistance of chicken lines to Campylobacter colonization have been reported and resistance-associated...
Sun, 03/15/2020 - 03:57
Author(s): S. Kumar, M. Singh, D.E. Cosby, N.A. Cox, H. Thippareddi Poultry processors use antimicrobials to reduce the risk of pathogens on poultry and poultry products. Efficacy of selective and nonselective plating media to enumerate injured Salmonella (selective media—brilliant green sulfa agar and Petrifilm Enterobacteriaceae Plate Count; nonselective media—tryptic soy agar and Petrifilm...
Tue, 03/10/2020 - 03:25
Campylobacter jejuni is a major foodborne pathogen worldwide. As it forms biofilms, it can become a persistent contaminant in the food and pharmaceutical industries. In this study, it was demonstrated that C. jejuni could make more biofilm in aerobic conditions than in microaerobic conditions, and only 13.9% C. jejuni entered coccus (a VBNC state) under microaerobic conditions; however, the rate...
Fri, 03/06/2020 - 03:46
Campylobacter jejuni is a widespread zoonotic pathogen and the leading bacterial cause of foodborne gastroenteritis in humans. Previous infection studies showed disruption of intercellular contacts, induction of epithelial apoptosis, and immune activation, all three contributing to intestinal barrier dysfunction leading to diarrhea. The present study aims to determine the impact of subepithelial...
Thu, 03/05/2020 - 03:27
Antimicrobial resistance (AMR) is a major public health threat worldwide. The main objective of this study was to compare AMR in Campylobacter from broiler chickens raised on Canadian farms and their products in different geographical regions of Canada. To do this, antimicrobial susceptibility results from isolates of Campylobacter recovered from a national microbiological baseline study...
Wed, 03/04/2020 - 04:07
Helical cell shape appears throughout the bacterial phylogenetic tree. Recent exciting work characterizing cell shape mutants in a number of curved and helical Proteobacteria is beginning to suggest possible mechanisms and provide tools to assess functional significance. We focus here on Caulobacter crescentus, Vibrio cholerae, Helicobacter pylori, and Campylobacter jejuni, organisms from three...
Wed, 03/04/2020 - 01:19
The economic burden of foodborne illness has been estimated to be as high as $90 billion annually.  For policy purposes, it is often important to understand not just the overall cost of illness, but also the costs associated with individual products or groups of products. In this study, I estimate the cost of foodborne illnesses from 29 pathogens associated with non-game meat and poultry products...
Wed, 03/04/2020 - 01:19
Campylobacter is one of the most commonly reported foodborne pathogens in the U.S. Because poultry is considered a major source of Campylobacter infections in humans, reducing Campylobacter contamination in poultry products is likely the most important and effective public health strategy to reduce the burden of campylobacteriosis in humans. A comprehensive on-line survey was conducted of key...
Wed, 03/04/2020 - 01:19
The burden of foodborne illness linked to the consumption of contaminated broiler meat is high in the United States. With the increase in popularity of alternative poultry rearing and production systems, it is important to identify the difference in food safety risks presented by alternative systems when compared to conventional methods. While many studies have been conducted surveying foodborne...
Wed, 03/04/2020 - 01:18
Campylobacter is one of the most commonly reported foodborne pathogens in the U.S. Because poultry is considered a major source of Campylobacter infections in humans, reducing Campylobacter contamination in poultry products is likely the most important and effective public health strategy to reduce the burden of campylobacteriosis in humans. A comprehensive on-line survey was conducted of key...
Wed, 03/04/2020 - 01:18
The burden of foodborne illness linked to the consumption of contaminated broiler meat is high in the United States. With the increase in popularity of alternative poultry rearing and production systems, it is important to identify the difference in food safety risks presented by alternative systems when compared to conventional methods. While many studies have been conducted surveying foodborne...
Tue, 03/03/2020 - 03:37
Increasing evidence exists for the role that cattle play in the epidemiology of campylobacteriosis. In this study, the prevalence and distribution of Campylobacter jejuni were longitudinally examined at the subspecies level in the beef cattle production continuum. Animals were subdivided into two groups: those that were not administered antibiotics and those that were administered the...
Tue, 03/03/2020 - 03:25
Campylobacter jejuni and Campylobacter coli are major food-borne pathogens that cause bacterial gastroenteritis in humans, and poultry is considered as their most important reservoir. Macrolides, such as erythromycin, are the first-line choice for treatment of campylobacteriosis. In this study, of the 143 Campylobacter isolates recovered from poultry in central China during 2015–2017, 25.2% were...
Wed, 02/26/2020 - 03:36
ABSTRACT Bacterial flagella are reversible rotary motors that rotate external filaments for bacterial propulsion. Some flagellar motors have diversified by recruiting additional components that influence torque and rotation, but little is known about the possible diversification and evolution of core motor components. The mechanistic core of flagella is the cytoplasmic C ring, which functions as...
Fri, 02/21/2020 - 03:51
Campylobacter jejuni is a major cause of bacterial gastroenteritis worldwide. In this study, we report the comparative genomic and functional characteristics of C. jejuni YH002 recently isolated from retail beef liver. Whole-genome sequencing and annotation of the strain revealed novel genetic features, including an integrated intact phage element, multiple antimicrobial resistance (AMR) genes,...
Thu, 02/20/2020 - 03:37
Author(s): Haejin Hwang, Richard E. Isaacson, Randall S. SingerAbstractCampylobacteriosis caused by C. jejuni is a serious yet common foodborne disease in the U.S. The prevalence of fluoroquinolone-resistant C. jejuni from poultry has continued to increase despite the withdrawal of fluoroquinolone use in the U.S. poultry industry in 2005. To date, no clear selective pressures that explain this...
Thu, 02/20/2020 - 03:36
Author(s): G. Rasschaert, L. De Zutter, L. Herman, M. HeyndrickxAbstractCampylobacter is one of the most important causative agents of foodborne illnesses worldwide. The poultry reservoir is the main source of campylobacteriosis. Within the broiler production chain, campylobacters can only multiply in the chicken’s intestinal tract. Intervention at farm level to reduce Campylobacter is thus...
Wed, 02/19/2020 - 03:43
by Mona Alzheimer, Sarah L. Svensson, Fabian König, Matthias Schweinlin, Marco Metzger, Heike Walles, Cynthia M. Sharma The Gram-negative Epsilonproteobacterium Campylobacter jejuni is currently the most prevalent bacterial foodborne pathogen. Like for many other human pathogens, infection studies with C. jejuni mainly employ artificial animal or cell culture models that can be limited in their...
Wed, 02/19/2020 - 03:43
In 2006, New Zealand had the highest notification rate of campylobacteriosis in the world, and poultry was considered the leading source of campylobacteriosis. Implementation of food safety interventions by the poultry industry led to a decrease in the campylobacteriosis notification rate. The aim is to examine the impact of targeted food safety interventions implemented by the New Zealand...
Wed, 02/19/2020 - 03:33
In the 1980s, I found that the chromosomal β‐lactamase of Klebsiella pneumoniae LEN‐1 showed a very high similarity to the R‐plasmid‐mediated penicillinase TEM‐1 on amino acid sequence level, and this strongly suggested the TEM‐1 originated from the chromosomal penicillinases of K. pneumoniae or related bacteria. Moreover, the chromosomal K1 β‐lactamase (KOXY) of Klebsiella oxytoca was found to...
Sun, 02/16/2020 - 03:57
Author(s): Jasmien Vandeputte, An Martel, Gunther Antonissen, Marc Verlinden, Lieven De Zutter, Marc Heyndrickx, Freddy Haesebrouck, Frank Pasmans, An GarmynAbstractOral administration of antibodies is a promising strategy against various infectious diseases. Previously, it was demonstrated that passive immunization by providing hyperimmune egg yolk through the feed reduces Campylobacter jejuni...
Sun, 02/16/2020 - 03:42
Food safety problems pose a great threat to the health of consumers with the greatest burden in developing countries. Street-vended foods play a key role in providing many urban dwellers with cheap, nutritious, and accessible food, but when prepared in an unhygienic and unregulated environment, they could contribute to increased food safety burden. The study investigated the microbiological...
Fri, 02/14/2020 - 03:37
Four wild-type Campylobacter jejuni strains isolated from the cecal contents of broiler chickens were sequenced. The average genome size was 1,622,170 bp, with 1,667 to 1,761 coding sequences and 47 to 51 RNAs. Multiple genes encoding motility, intestinal colonization, toxin production, stress tolerance, and multidrug resistance were present in all the strains.
Fri, 02/14/2020 - 03:35
Author(s): Kinga Wieczorek, Łukasz Bocian, Jacek OsekAbstractThe aim of the present study was to assess the prevalence of Campylobacter on chicken carcasses tested in Poland during 2014–2018 and to evaluate the antimicrobial susceptibility of the recovered isolates. The results were compared to the previous similar investigations performed in 2009–2013 [Wieczorek K. & Osek J. (2015). A five-...
Thu, 02/13/2020 - 04:06
Intensive poultry production due to public demand raises the risk of contamination, creating potential foodborne hazards to consumers. The prevalence and microbial load of the pathogens Campylobacter, Salmonella, Staphylococcus aureus, and Escherichia coli was determined by standard methods at the farm level. After disinfection, swab samples collected from wall crevices, drinkers, and vents were...
Wed, 02/12/2020 - 10:49
Author(s): Shaban Rahimi, Sophia Kathariou, Oscar Fletcher, Jesse L. GrimesAbstractSalmonella and Campylobacter are considered major public health burdens worldwide, and poultry are known to be one of the main reservoirs for these zoonotic pathogens. This study was conducted to evaluate the effect of a commercial probiotic or direct-fed microbial (DFM) Calsporin (CSP), and prebiotic or mannan...
Wed, 02/12/2020 - 03:24
Campylobacter jejuni (C. jejuni) has been assigned as an important food-borne pathogen for human health but many pathogenicity factors of C. jejuni and human host cell responses related to the infection have not ...
Sat, 02/08/2020 - 03:24
The in vitro resistance of selected red/orange complex periodontal pathogens to tinidazole was compared with four other antibiotics. Subgingival biofilm samples from 88 adults with severe periodontitis were anaerobically incubated on enriched Brucella blood agar with and without supplementation with tinidazole (16 mg/L), metronidazole (16 mg/L), amoxicillin (8 mg/L), doxycycline (4 mg/L), or...
Thu, 02/06/2020 - 03:26
K. G. Joensen et al. In industrialized countries, the leading cause of bacterial gastroenteritis is Campylobacter jejuni. However, outbreaks are rarely reported, which may reflect limitations of surveillance, for which molecular typing is not routinely performed. To determine the frequency of genetic clusters among patients and to find links to concurrent isolates from poultry meat, broiler...

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