Campylobacter

Tue, 02/04/2020 - 04:10
The gut-lymph node axis is a critical player in the symbiotic relationship between gut microbiota and the host. However, little is known about the impact of diet-related bacterial shifts in the gut lumen on bacterial translocation into lymph nodes. Here, we (i) characterized changes in the viable microbiota composition along the ileal digesta-mucosa-lymph node axis and (ii) examined the effect of...
Thu, 01/30/2020 - 03:36
Pathogenic microorganisms use various mechanisms to conserve energy in host tissues and environmental reservoirs. One widespread but often overlooked means of energy conservation is through the consumption or production of molecular hydrogen (H2). Here, we comprehensively review the distribution, biochemistry, and physiology of H2 metabolism in pathogens. Over 200 pathogens and pathobionts carry...
Thu, 01/30/2020 - 03:25
Foodborne infections, mainly those attributable to Campylobacter, are one of the most common causes of intestinal diseases, of bacterial origin in humans. Although the vehicle of transmission is not always identified, the most common vehicles are poultry, poultry products, and contaminated water. In Southern Benin, an excessive use of poultry manure as fertilizer in vegetable farms was noted....
Wed, 01/29/2020 - 03:43
The survey was undertaken to investigate the presence and antimicrobial susceptibility profile of Salmonella spp. in raw and ready-to-eat (RTE) foods, and Campylobacter spp. in the retail raw chicken meat collected in two counties of Transylvania, Romania. A total of 13.1% (51/388) of the examined food samples were found to be Salmonella positive, with a distribution of 14.7% (48/326) in the raw...
Sun, 01/26/2020 - 03:27
The aim of this study was to investigate if HtrA is responsible for differences in adherence and invasion of Campylobacter jejuni towards human and chicken cell lines. Gentamicin protection assays were performed with either human Caco‐2 or chicken 2G4 cells using C. jejuni strain NCTC11168 to compare the adhesion and invasion rates towards these two cell types. The results revealed significant...
Fri, 01/24/2020 - 03:34
The complete genome sequence of Campylobacter jejuni YH003, isolated from retail chicken, was determined using PacBio and Illumina technologies. The assembled genome is 1,743,985 bp (G+C content of 30.3%). Genome annotation revealed several genes encoding virulence and antibiotic resistance factors, including a type VI secretion system, cytolethal distending toxins, and a multidrug efflux system.
Fri, 01/24/2020 - 03:24
Poultry is a major reservoir for the pathogen Campylobacter jejuni. C. jejuni inhabits the poultry gastrointestinal tract as a part of the gut microbiota. The objective of this study was to evaluate both the survival of C. jejuni and the changes in the population dynamics of the cecal microbiome during an in vitro C. jejuni inoculation in the presence or absence of the functional metabolites of...
Wed, 01/22/2020 - 03:28
A newly isolated Campylobacter jejuni bacteriophage CJ01 was partially characterized and might be a potential biocontrol agent to improve food safety. Abstract Campylobacter jejuni is a major foodborne pathogen causing acute bacterial enteritis worldwide. Screening of novel bacteriophages as biocontrol tools to eliminate C. jejuni in food during pre‐ and postharvest is an emerging research field...
Wed, 01/22/2020 - 03:24
This study investigated the antibiotic resistance, virulence profiles, and clonality of Campylobacter jejuni and Campylobacter coli isolated from an intensive poultry farming system in KwaZulu-Natal, South Africa. Following ethical approval, samples were collected over six weeks using the farm-to-fork approach. Campylobacter spp. were identified using culture, confirmed and differentiated to...
Sat, 01/18/2020 - 03:33
by Mohamed Elhadidy, Mohamed Medhat Ali, Ayman El-Shibiny, William G. Miller, Walid F. Elkhatib, Nadine Botteldoorn, Katelijne Dierick The aim of this study is to characterize the antimicrobial resistance of Campylobacter jejuni recovered from diarrheal patients in Belgium, focusing on the genetic diversity of resistant strains and underlying molecular mechanisms of resistance among Campylobacter...
Thu, 01/16/2020 - 03:33
ABSTRACT Campylobacter jejuni is a leading cause of bacterial diarrhea worldwide and is associated with high rates of mortality and growth stunting in children inhabiting low- to middle-resource countries. To better understand the impact of breastfeeding on Campylobacter infection in infants in sub-Saharan Africa and South Asia, we examined fecal microbial compositions, bacterial isolates, and...
Wed, 01/15/2020 - 03:23
This study investigated occurrence and antimicrobial resistance profiles of Campylobacter spp. isolates in beef cattle on five cow–calf operations in South Africa. A total of 537 fecal samples from adult beef cattle (n = 435) and rectal swabs from calves (n = 102) were screened for Campylobacter jejuni, Campylobacter coli, and Campylobacter upsaliensis by culture and polymerase chain reaction....
Tue, 01/14/2020 - 03:34
Campylobacter jejuni causes acute gastroenteritis world-wide and is transmitted primarily through poultry, in which it is often a commensal member of the intestinal microbiota. Previous RNASeq experiments showed that transcripts from an operon encoding a high affinity phosphate transporter (PstSCAB) of C. jejuni were among the most abundant when grown in chickens. Elevated levels of the pstSCAB...
Tue, 01/14/2020 - 03:23
Campylobacter jejuni is one of the most common zoonotic pathogens worldwide. Although the main sources of human C. jejuni infection are livestock, wildlife can also affect C. jejuni transmission in humans. However, it remains unclear whether wild mice harbor C. jejuni and are involved in the “environment–wildlife–livestock–human” transmission cycle of C. jejuni in humans. Here, we characterized C...
Sat, 01/11/2020 - 03:25
Aim Following previous research on improving the cleaning of crates used to transport broiler chickens from the farm to the abattoir, a demonstration project was undertaken to investigate improvements in crate washing on a commercial scale. Methods and Results The soak tank of a conventional crate washing system was replaced with a high‐performance washer fitted with high‐volume, high‐pressure...
Fri, 01/10/2020 - 03:30
Author(s): Anastasia E. Lytou, Chrissanthi T. Renieri, Agapi I. Doulgeraki, George-John E. Nychas, Efstathios Z. PanagouAbstractThe prevalence of three pathogens in marinated chicken products and the evaluation of their quality by microbiological and sensory analysis were assessed. Eighty (80) samples obtained from several meat retail markets in Greece were analyzed for the presence of...
Fri, 01/10/2020 - 03:23
Campylobacter can enter a viable but non-culturable (VBNC) state to evade various stresses, and this state is undetectable using traditional microbiological culturing techniques. These VBNC bacterial cells retain metabolism and demonstrate pathogenic potential due to their ability to resuscitate under favorable conditions. Rapid and accurate determination of VBNC Campylobacter is critical to...
Thu, 01/09/2020 - 04:03
Author(s): Torrung Vetchapitak, Taisuke Shinki, Satomi Sasaki, Takako Taniguchi, Taradon Luangtongkum, Naoaki MisawaAbstractThe present study was conducted to evaluate the efficacy of a new technique for removal of campylobacters from naturally contaminated free-range and broiler chicken carcasses. The carcasses, obtained from commercial processing plants after evisceration, were immersed in 0.1...
Thu, 01/09/2020 - 03:53
Campylobacter species are known as biofilm‐forming bacteria in food systems. The aim of this study was to evaluate the antimicrobial and antibiofilm effects of cinnamaldehyde against Campylobacter jejuni and Campylobacter coli isolated from chicken meat. The biofilm‐forming C. jejuni and C. coli strains from chicken meat were investigated using minimum inhibitory concentration (MIC) and...
Thu, 01/09/2020 - 03:42
The prevalence of human infections with the zoonotic pathogen Campylobacter jejuni is rising worldwide. Therefore, the identification of compounds with potent anti-pathogenic and anti-inflammatory properties for ...
Fri, 01/03/2020 - 04:13
Campylobacter jejuni is the main cause of bacterial foodborne disease in humans, who are exposed mostly by consumption of contaminated poultry products. C. jejuni strain NADC 20827 was isolated from the feces of turkeys naturally colonized with Campylobacter spp. We present the complete annotated genome and plasmid sequences of strain NADC 20827.
Fri, 01/03/2020 - 04:13
Campylobacter jejuni is a major foodborne pathogen that plays an important role in spreading drug resistance. We report the draft genome sequences of two multidrug-resistant C. jejuni isolates which contained similar mutations in the CmeR box. This will improve the understanding of C. jejuni antimicrobial resistance and genetic characteristics.
Fri, 01/03/2020 - 04:13
Campylobacter jejuni is a foodborne pathogen and an important contributor to gastroenteritis in humans. C. jejuni readily forms biofilms which may play a role in the transmission of the pathogen from animals to humans. Herein, we present RNA sequencing data investigating differential gene expression in biofilm and planktonic C. jejuni. These data provide insight into pathways which may be...
Wed, 12/25/2019 - 03:32
Author(s): Trond Møretrø, Lydia Martens, Paula Teixeira, Vânia B. Ferreira, Rui Maia, Tove Maugesten, Solveig LangsrudAbstractCleaning is a method at the disposal of domestic cooks for curtailing the dispersal of foodborne pathogens in the process of preparing food. The observation of visible dirt/soil ‘in the wrong place’ operates as one of the stimuli for action. This paper makes a...
Sat, 12/21/2019 - 04:03
Author(s): Eric D. Ebel, Michael S. Williams, Denise M. AmannAbstractWhen interpreting the status of poultry processing establishments subject to performance standards testing, USDA-FSIS employs a round-up rule when fewer than the standard 52 samples per establishment per year are collected. This rule is to ensure that an establishment with fewer than 52 samples is held to a performance standard...
Thu, 12/19/2019 - 03:27
Campylobacter jejuni is the one of the leading cause of bacterial food borne gastroenteritis. PglB, a glycosyltransferase, plays a crucial role of mediating glycosylation of numerous periplasmic proteins. It catalyzes N-glycosylation at the sequon D/E-X1-N-X2-S/T in its substrate proteins. Here we report that the PglB itself is a glycoprotein which self-glycosylates at N534 site in its DYNQS...
Wed, 12/18/2019 - 04:04
Author(s): Noritoshi Hatanaka, Sharda Prasad Awasthi, Hisataka Goda, Hiroyuki Kawata, Yuzuru Uchino, Takahiro Kubo, Shigeru Aoki, Atsushi Hinenoya, Shinji YamasakiAbstractFoodborne disease caused by campylobacters is one of the major global problems for food safety. Infection source of Campylobacter to human is mainly through contaminated meat particularly chicken. Contamination of meat with...
Wed, 12/18/2019 - 03:43
Campylobacter jejuni is a zoonotic pathogen that infects the human gut through the food chain mainly by consumption of undercooked chicken meat, raw chicken cross-contaminated ready-to-eat food or by raw milk. In...
Tue, 12/17/2019 - 03:33
Author(s): Patrizia Casagrande Proietti, Gabriella Guelfi, Sara Bellucci, Silvio De Luca, Settimio Di Gregorio, Camillo Pieramati, Maria Pia FranciosiniAbstractThe objectives of this work were to evaluate β-lactamase-mediated β-lactam resistance in Campylobacter coli and Campylobacter jejuni isolates obtained from broiler chickens, expression of the blaOXA-61 gene in relation to β-lactamase...
Tue, 12/17/2019 - 03:25
The role of the Type VI secretion system (T6SS) in Campylobacter jejuni is poorly understood despite an increasing prevalence of the T6SS in recent C. jejuni isolates in humans and chickens. The T6SS is a contractile secretion machinery capable of delivering effectors that can play a role in host colonization and niche establishment. During host colonization, C. jejuni is exposed to oxidative...
Tue, 12/17/2019 - 03:25
Foodborne infections, mainly those attributable to Campylobacter, are one of the most common causes of intestinal diseases, of bacterial origin in humans. Although the vehicle of transmission is not always identified, the most common vehicles are poultry, poultry products, and contaminated water. In Southern Benin, an excessive use of poultry manure as fertilizer in vegetable farms was noted....
Sat, 12/14/2019 - 03:33
Author(s): Jong Min Yang, Gi Eob Kim, Kyeong Rok Kim, Chang Sup KimAbstractThe successful enzymatic synthesis of various ganglioside-related oligosaccharides requires many available glycan-processing enzymes. However, the number of available glycan-processing enzymes remains limited. In this study, the full-length CgtA43456 (β-(1→4)-N-acetylgalactosaminyltransferase) and CgtB11168 (β-(1→3)-...
Sat, 12/07/2019 - 03:38
by Elisabetta Di Giannatale, Paolo Calistri, Guido Di Donato, Lucia Decastelli, Elisa Goffredo, Daniela Adriano, Maria Emanuela Mancini, Annamaria Galleggiante, Diana Neri, Salvatore Antoci, Cristina Marfoglia, Francesca Marotta, Roberta Nuvoloni, Giacomo Migliorati Campylobacter species are common foodborne pathogens associated with cases of human gastroenteritis worldwide. A detailed...
Fri, 12/06/2019 - 03:38
by Raies A. Mir, Robert G. Schaut, Heather K. Allen, Torey Looft, Crystal L. Loving, Indira T. Kudva, Vijay K. Sharma Vaccination-induced Escherichia coli O157:H7-specific immune responses have been shown to reduce E. coli O157:H7 shedding in cattle. Although E. coli O157:H7 colonization is correlated with perturbations in intestinal microbial diversity, it is not yet known whether vaccination...
Thu, 12/05/2019 - 04:17
by Julian E. Grass, Sunkyung Kim, Jennifer Y. Huang, Stephanie M. Morrison, Andre E. McCullough, Christy Bennett, Cindy R. Friedman, Anna Bowen Gastrointestinal illnesses are the most frequently diagnosed conditions among returning U.S. travelers. Although most episodes of travelers’ diarrhea do not require antibiotic therapy, fluoroquinolones (a type of quinolone antibiotic) are recommended for...
Thu, 11/28/2019 - 04:13
Campylobacter jejuni, a leading cause of gastroenteritis worldwide, has been frequently isolated from recreational rivers and streams in New Zealand, yet the public health significance of this is unknown. This study uses molecular tools to improve our understanding of the epidemiology and sources of Campylobacter in recreational waterways, with a view to preventing human infection....
Sat, 11/23/2019 - 03:36
Author(s): Els Vossen, Sophie Goethals, Jo De Vrieze, Nico Boon, Thomas van Hecke, Stefaan De SmetAbstractPigs were fed either red and processed meat or chicken meat within either a prudent or a Western dietary pattern for four weeks (2 × 2 full factorial design). The colon microbial community and volatile organic compounds were assessed (either quantified or based on their presence). Results...
Fri, 11/22/2019 - 03:26
Salmonella and Campylobacter are leading human foodborne pathogens commonly associated with poultry and poultry products, and several methods to control these pathogens have been applied to poultry production. This study was conducted to evaluate the effect of CALSPORIN, (CSP), a direct-fed microbial (DFM), and yeast cell wall (Saccharomyces cervisiae, IMW50, a mannanoligosaccharide (MOS)-based...
Fri, 11/22/2019 - 03:26
ABSTRACT Dietary prebiotics are thought to be potentially important alternatives to antibiotic growth promoters in poultry production because of their beneficial performance and health effects. The administration of dietary prebiotics has been demonstrated to improve animal health, growth performance, and microbial food safety in poultry production. In this study, we evaluated the effects of...
Tue, 11/19/2019 - 03:38
Campylobacter jejuni is a leading cause of foodborne illnesses worldwide. Its porA gene encodes the major outer membrane protein (MOMP) that is abundantly expressed and has important physiological functions, including a key role in systemic infection and abortion induction in pregnant animals. Despite the importance of porA in C. jejuni pathogenesis, mechanisms modulating its expression levels...
Sun, 11/17/2019 - 03:24
The aim of this study was to investigate the prevalence and distribution of Campylobacter species in a variety of fresh and frozen meat and offal products collected from retail outlets in New South Wales (NSW), Queensland (Qld), and Victoria (Vic). A total of 1,490 chicken, beef, lamb, and pork samples were collected from Australian supermarkets and butcher shops over a 2-year sampling period (...
Fri, 11/15/2019 - 03:32
Author(s): Zifeng Han, Li Li, Thomas Willer, Wolfgang Baumgärtner, Silke RautenschleinAbstractCampylobacter jejuni (C. jejuni) is a predominant cause of foodborne illness in humans, while its colonization in chickens is usually asymptomatic. Antibiotics are not routinely used to treat chickens against C. jejuni, but in the face of other bacterial diseases, C. jejuni may be exposed to antibiotics...
Thu, 11/14/2019 - 03:36
ABSTRACT O-linked protein glycosylation is a conserved feature of the Burkholderia genus. The addition of the trisaccharide β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc to membrane exported proteins in Burkholderia cenocepacia is required for bacterial fitness and resistance to environmental stress. However, the underlying causes of the defects observed in the absence of glycosylation are...
Thu, 11/14/2019 - 03:25
Campylobacter jejuni is the leading cause of human foodborne enteritis worldwide. Poultry products are regarded as the main source of human campylobacteriosis. Strategies are being developed to reduce colonization of poultry by Campylobacter. The membrane transport protein CjaA was reported to stimulate mucosal immune responses, which can reduce the C. jejuni load in chickens. In this study, oral...
Mon, 11/11/2019 - 03:26
A matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) method were established to detect Campylobacter jejuni and Listeria monocytogenes from a chicken farm and samples from commercial live chickens. Suspected isolates were pretreated and crystallized by matrix solutions consisting of ethanol, acetonitrile, and water with different proportions. The effect of...
Sun, 11/10/2019 - 03:26
Aims Current culture‐based methods for detection and determination of Campylobacter levels on processed chickens takes at least two days. Here, we sought to develop a new complete, low‐cost and rapid (approximately 2·5 hours) detection system requiring minimal operator input. Methods and Results We observed a strong correlation between culture‐based cell counts and our ability to detect either...
Fri, 11/08/2019 - 03:39
by Francesca Marotta, Giuliano Garofolo, Lisa Di Marcantonio, Gabriella Di Serafino, Diana Neri, Romina Romantini, Lorena Sacchini, Alessandra Alessiani, Guido Di Donato, Roberta Nuvoloni, Anna Janowicz, Elisabetta Di Giannatale
Thu, 11/07/2019 - 03:29
This study investigated the occurrence of shiga toxin‐producing Escherichia coli (STEC), thermotolerant Campylobacter spp. and Salmonella spp. in Swedish dairy milk. A total of 302 inline milk filters were analyzed. Salmonella was not isolated from any filters. Polymerase chain reaction screening detected thermotolerant Campylobacter in 30.5% of the milk filters analyzed and it was isolated from...
Wed, 11/06/2019 - 03:26
SUMMARY Microbiological safety and quality of fresh chicken meat are important concerns to industry. The objective of this study was to investigate the effects of in-package, non-thermal high voltage dielectric barrier discharge (HVDBD) treatment on microbial quality, safety, and color of fresh chicken breast meat (pectoralis major). Boneless skinless chicken breast meat was collected from a...
Wed, 11/06/2019 - 03:26
SUMMARY Salmonella and Campylobacter are the two pathogens commonly associated with raw poultry meat, as poultry products are frequent vehicles of these bacteria. The objective of the current research was to determine the optimal contact time for 6 antimicrobial treatments, including water, 0.003% chlorine, 0.07% peracetic acid (PAA), 0.1% PAA, 0.35% cetylpyridinium chloride (CPC), and 0.6% CPC...

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