Clostridium perfringens

Thu, 03/19/2020 - 03:31
Author(s): R. Shanmugasundaram, A. Markazi, M. Mortada, T.T. Ng, T.J. Applegate, L.R. Bielke, B. Syed, C.M. Pender, S. Curry, G.R. Murugesan, R.K. Selvaraj Studies were conducted to determine the efficacy of synbiotic applications to combat the negative effects of necrotic enteritis (NE). An in vitro study was conducted to test the effect of probiotics species supernatants to decrease Clostridium...
Sun, 03/01/2020 - 03:33
Author(s): Mahmoud Fayez, Ibrahim Elsohaby, Theeb Al-Marri, Kamal Zidan, Ali Aldoweriej, Elham El-Sergany, Ahmed ElmoslemanyThe present study aimed to isolate and genotype C. perfringens from healthy and diarrheic dromedary camels, pastures and herders; and to evaluate and compare antimicrobial susceptibility of the isolates. A total of 262 (56.3%) C. perfringens isolates were recovered from 465...
Sun, 02/23/2020 - 03:35
Author(s): M. Hilliar, C. Keerqin, C.K. Girish, R. Barekatain, S.-B. Wu, R.A. SwickAbstractNecrotic enteritis (NE) is an infection of the gastrointestinal tract and is estimated to cost the global poultry industry billions of dollars annually. A study was conducted to examine whether reducing the crude protein might offset the severity of NE in broilers experimentally challenged with Eimeria spp...
Sat, 02/15/2020 - 03:36
Author(s): Ryunkyoung Oh, Min Jeong Lee, Young-Ok Kim, Bo-Hye Nam, Hee Jeong Kong, Ju-Won Kim, Jung-yeon Park, Jung-Kil Seo, Dong-Gyun KimAbstractWe isolated and purified an antimicrobial peptide (AMP) from the mantle of the hard-shelled mussel, Mytilus coruscus. The peptide was purified through C18 reversed-phase high-performance liquid chromatography, and displayed antibacterial activity. Total...
Wed, 02/12/2020 - 10:49
Author(s): Uraisha Ramlucken, Santosh O. Ramchuran, Ghaneshree Moonsamy, Rajesh Lalloo, Mapitsi S. Thantsha, Christine Jansen van RensburgABSTRACTThere is a necessity for the implementation of in-feed probiotics in the poultry production industry, following strict regulations around the use of antibiotic growth promoters (AGP). Bacillus spp. are becoming an attractive alternative because of their...
Wed, 02/12/2020 - 10:49
Author(s): H.K. Zanu, C. Keerqin, S.K. Kheravii, N.K. Morgan, S-B. Wu, M.R. Bedford, R.A. SwickAbstractThis study investigated the influence of meat and bone meal (MBM), phytase, and antibiotics (AB) on the performance, intestinal pH, ileal digestibility, cecal microbiota, and tibial mineralization in Ross 308 broilers challenged with necrotic enteritis (NE). A total of 672-day-old male Ross 308...
Fri, 02/07/2020 - 03:53
Aims The purpose of this study was to isolate Lactobacillus from gastrointestinal tract of healthy post‐weaning piglets and investigate its synergistic antimicrobial and probiotic effects with ZnO nanoparticles (nZnO). Methods and Results Out of the 128 isolates, L. plantarum BLPL03 was selected based on its excellent acid and bile salt tolerance properties. L. plantarum BLPL03 was sensitive to β...
Fri, 01/31/2020 - 03:29
Salmonella enterica, Listeria monocytogenes, Shigella flexneri, Escherichia coli O157:H7, Vibrio parahaemolyticus, Staphylococcus aureus, Vibrio cholerae, Clostridium botulinum type A, Bacillus cereus, Clostridium perfringens Alpha toxin, and Yersinia enterocolitica are 11 common foodborne pathogens. Traditional bacterial culture methods for detecting pathogens are time‐consuming and labor‐...
Thu, 01/30/2020 - 03:36
Pathogenic microorganisms use various mechanisms to conserve energy in host tissues and environmental reservoirs. One widespread but often overlooked means of energy conservation is through the consumption or production of molecular hydrogen (H2). Here, we comprehensively review the distribution, biochemistry, and physiology of H2 metabolism in pathogens. Over 200 pathogens and pathobionts carry...
Thu, 01/30/2020 - 03:32
Author(s): Hani El Kadri, Teyfik Celen, Alaa Alaizoki, Madeleine Smith, Helen OnyeakaAbstractLow-temperature long-time (LTLT) cooking may lead to risk of potential survival of pathogenic bacteria such as Clostridium perfringens in cooked meat. In this study, the effect of LTLT cooking on C. perfringens was investigated at temperatures commonly used by caterers. Brain heart infusion broth (BHIB)...
Sat, 01/25/2020 - 03:30
LWT
Author(s): Padmanabha Reddy Velugoti, Saurabh Kumar, Lalit Kumar Bohra, Vijay K. Juneja, Harshavardhan ThippareddiAbstractInhibition of Clostridium perfringens spore germination and outgrowth by buffered calcium, potassium, and sodium citrates (Ca-Cit, K-Cit, and Na-Cit, respectively) during exponential cooling of non-cured and cured pork was evaluated. Antimicrobials were added to a base...
Fri, 01/24/2020 - 03:34
Clostridium perfringens causes severe gastrointestinal diseases, which include necrotic enteritis (NE) in chickens, a deadly disease worldwide. We report here the draft genome sequence of Clostridium perfringens strain TAMU, which was used in developing an NE chicken challenge model. This C. perfringens TAMU genome sequence will aid in advancing potential intervention strategies to reduce NE...
Sun, 01/19/2020 - 03:33
Author(s): Jackie K. Cheung, Vicki Adams, Danielle D’Souza, Meagan James, Christopher J. Day, Michael P. Jennings, Dena Lyras, Julian I. RoodAbstractClostridium perfringens is the causative agent of human clostridial myonecrosis; the major toxins involved in this disease are α-toxin and perfringolysin O. The RevSR two-component regulatory system has been shown to be involved in regulating...
Sat, 01/18/2020 - 03:28
Author(s): L. Grau-Roma, S. Blatter, C. Wenker, K. Sonja, M. Navarro, F. Uzal, H. PosthausIntroduction: Clostridium perfringens is the cause of necrotic enteritis (NE) in chickens. A similar disease has occasionally been reported in psittacines. We diagnosed several outbreaks of NE in lorikeets (Trichoglossus haematodus).
Tue, 01/14/2020 - 03:23
Clostridium perfringens (CP) is a foodborne pathogen. The bacterium can also inhabit human gut without symptoms of foodborne illness. However, the clinical symptoms of long-term inhabitation have not been known yet. Therefore, the objective of this study was to elucidate the relationship between intestinal CP and other internal organs. Phosphate-buffered saline (PBS) and CP were orally injected...
Sat, 01/11/2020 - 03:23
Clostridium perfringens is an important human and animal pathogen that is the primary causative agent of necrotizing enteritis and enterotoxemia in many types of animals; it causes traumatic gas gangrene in humans and animals and is associated with cases of food poisoning in humans. C. perfringens produces a variety of toxins as well as many enzymes, including three sialidases, NanH, NanI, and...
Fri, 01/10/2020 - 03:30
Author(s): Yaodi Zhu, Jiaye Zhang, Miaoyun Li, Lijun Zhao, Hongrong Ren, Longgnag Yan, Gaiming Zhao, Chaozhi ZhuAbstractThe Gram-positive, anaerobic, spore-forming bacterium, Clostridium perfringens (C. perfringens) causes a variety of diseases in humans and other animals. Spore germination is thought to be the first stage of infection by C. perfringens. AGFK, a mixture of l-asparagine, d-glucose...
Thu, 01/09/2020 - 04:04
Author(s): Simon Packer, Jane Day, Peter Hardman, Julia Cameron, Michael Kennedy, Jonathan Turner, Caroline Willis, Corinne Amar, Bayad Nozad, Maya GobinAbstractIn March 2018, Public Health England was alerted to an outbreak of gastrointestinal illness in persons who had eaten at a restaurant in the South West of England on Mothering Sunday. We aimed to determine the source of infection and...
Wed, 01/08/2020 - 03:37
Author(s): Zhen Jia, Yanhong Liu, Chang-An Hwang, Lihan HuangAbstractClostridium perfringens is a strictly anaerobic pathogen that requires absence of oxygen for its growth in laboratory experiments, which is usually attained by using an anaerobic chamber or anaerobic jars. However, it has been demonstrated that C. perfringens may survive for short periods of times due to its adaptive response to...
Tue, 01/07/2020 - 03:26
Epsilon toxin (ETX), produced by Clostridium perfringens types B and D, causes serious neurological disorders in animals. ETX can bind to the white matter of the brain and the oligodendrocytes, which are the cells forming the myelin sheath around neuron axons in the white matter of the central nervous system. After binding to oligodendrocytes, ETX causes demyelination in rat cerebellar slices. We...
Wed, 12/25/2019 - 03:34
ABSTRACT Clostridium perfringens type F strains cause gastrointestinal disease when they produce a pore-forming toxin named C. perfringens enterotoxin (CPE). In human enterocyte-like Caco-2 cells, low CPE concentrations cause caspase-3-dependent apoptosis, while high CPE concentrations cause necrosis. Since necrosis or apoptosis sometimes involves receptor-interacting serine/threonine-protein...
Fri, 11/22/2019 - 03:26
ABSTRACT Poultry meats can become contaminated with pathogenic bacteria through digesta leakage during processing. Reducing the bacteria load in digesta of market-aged broilers prior to processing reduces the incidence of fecal contamination at the processing plant. A lysozyme product was incorporated in a maltodextrin-based feed offered during the pre-shipping feed withdrawal period to reduce...
Fri, 11/22/2019 - 03:26
ABSTRACT Necrotic enteritis (NE) is a pervasive enteric disease responsible for large scale economic losses within the global poultry industry. The etiologic agent of NE is Clostridium perfringens (CP), an opportunistic pathogen that utilizes numerous extracellular toxins and glycoside hydrolases (GH) as key virulence and nutrient acquisition factors. Notably, some GH, mucinases, degrade...
Fri, 11/22/2019 - 03:26
ABSTRACT Subclinical necrotic enteritis (NE) is primarily caused by the gram-positive bacterium, Clostridium perfringens (Cp). The trend towards removal of in-feed antimicrobials and subsequent increased emergence of infection in poultry has resulted in a wide interest in better understanding of the mechanism behind this disease. The virulence of NE, to a large extent, depends on the virulence...
Fri, 11/15/2019 - 03:28
This study aimed to assess the microbial contamination associated with the traditional processing of fresh pork into grilled pork in Benin. Sixty meat samples (fresh and processed pork) were randomly collected from different processing/selling sites, and the main foodborne microorganisms were sought. About 16.7% of samples exceeded the acceptable limit of <7.0 Log10 CFU g−1 recommended by the...
Sat, 11/09/2019 - 03:37
by Jennifer R. Linden, Claudia Flores, Eric F. Schmidt, Francisco A. Uzal, Adam O. Michel, Marissa Valenzuela, Sebastian Dobrow, Timothy K. Vartanian Clostridium perfringens epsilon toxin (ETX) is responsible for causing the economically devastating disease, enterotoxaemia, in livestock. It is well accepted that ETX causes blood brain barrier (BBB) permeability, however the mechanisms involved in...
Sat, 11/09/2019 - 03:25
Clostridium perfringens is a bacterial pathogen that causes necrotic enteritis in poultry and livestock, and is a source of food poisoning and gas gangrene in humans. As the agriculture industry eliminates the use of antibiotics in animal feed, alternatives to antibiotics will be needed. Bacteriophage endolysins are enzymes used by the virus to burst their bacterial host, releasing bacteriophage...
Wed, 11/06/2019 - 03:26
SUMMARY In a global context of increased antibiotic resistance, feed additives with enhanced antimicrobial properties are a useful and increasingly needed strategy. Organic acids (OA) and botanical molecules such as nature identical compounds (NIC) have been shown to be effective against bacterial infections based on their antimicrobial activity. The aim of this study was to evaluate whether the...
Tue, 11/05/2019 - 03:37
Author(s): Yaodi Zhu, Yangyang Ma, Jiaye Zhang, Miaoyun Li, Longgang Yan, Gaiming Zhao, Yanxia Liu, Yanyan ZhangAbstractIn this study, the effects of spices essential oils on the growth of C. perfringens were investigated. The in vitro inhibitory activities and minimum inhibitory concentrations (MIC) of different essential oils on C. perfringens were determined using the Oxford cup and two-fold...
Sat, 10/26/2019 - 03:34
Clostridium perfringens (C. perfringens) is Gram-positive anaerobic, spore-forming rod-shaped bacterial pathogen that is widely distributed in nature. This bacterium is known as the causative agent of a foodborne illness and of gas gangrene. While the major virulence factors are the α-toxin and perfringolysin O (PFO) produced by type A strains of C. perfringens, the precise mechanisms of how...
Tue, 10/15/2019 - 03:34
Besides the major nutrients, phosphorus (P) is an essential mineral for both the host animal and the porcine gut microbiota. Different strategies including phytase supplementation and more recently lactic acid (LA) are used to enhance the P availability from cereals in pig diets; however, their impact on the gut microbiota has been rarely related to fecal shedding of opportunistic pathogens. The...
Tue, 10/15/2019 - 03:34
Both nosocomial pathogens, such as Streptococcus pneumoniae and Haemophilus influenzae and food-borne pathogens, such as Bacillus cereus and Clostridium perfringens are known to be detrimental to human and animal health. The effectiveness of currently used treatments for these pathogens becomes limited as resistant strains emerge. Therefore, new methods for eliminating bacterial pathogens must be...
Fri, 10/11/2019 - 03:29
Necrotic enteritis (NE) causes huge economic losses to the poultry industry. Probiotics are used as potential alternatives to antibiotics to prevent NE. It is known that Clostridium butyricum can act as a probiotic that can prevent infection. However, whether or not it exerts a beneficial effect on NE in chickens remains elusive. Therefore, we investigated the impact of C. butyricum on immune...
Thu, 10/10/2019 - 03:29
Clostridium perfringens enterotoxin (CPE) contributes to diarrhea and an often-lethal enterotoxemia. CPE action starts when it binds to claudin receptors, forming a small complex (90 kDa). Six small complexes then oligomerize to create prepores, followed by insertion of beta-hairpins from CPE to form beta-barrel pores named CH-1 or CH-2. Of the ~27 members of the human claudin protein family,...
Wed, 10/02/2019 - 03:30
Clostridium perfringens type A causes gas gangrene characterized by myonecrosis and development of an effective therapy for treating affected patients is of clinical importance. It was recently reported that the expression of granulocyte colony-stimulating factor (G-CSF) is greatly up-regulated by C. perfringens infection. However, the role of G-CSF in C. perfringens-mediated myonecrosis is still...
Wed, 10/02/2019 - 03:30
Clostridium is a broad genus of anaerobic, spore-forming, rod-shaped, Gram-positive bacteria that can be found in different environments all around the world. The genus includes human and animal pathogens that produce potent exotoxins that cause rapid and potentially fatal diseases responsible for countless human casualties and billion-dollar annual loss to the agricultural sector. Diseases...
Tue, 10/01/2019 - 03:39
Author(s): A. Schoster, T. Kunz, M. Lauper, C. Graubner, S. Schmitt, J.S. WeeseAbstractOvergrowth of enteric clostridia in dysbiosis in horses with colic is presumed but scarcely investigated. The objective was to provide prevalence data of Clostridium difficile and Clostridium perfringens in horses with and without gastrointestinal disease in Switzerland, and investigate microbiota differences...
Fri, 09/20/2019 - 03:30
Clostridium perfringens toxinotype D, toxinotype E, and gastroenteritis-linked BEC/CPILE-positive strains have never been reported in healthy children. We isolated, whole-genome sequenced and bioinformatically characterised three C. perfringens isolates&mdash;type D (IQ1), type E (IQ2) and BEC/CPILE-positive (IQ3), recovered from the stools of three healthy two-year-olds, which were further...
Thu, 09/19/2019 - 00:00
Toxins, Vol. 11, Pages 543: Genomic Analysis of Clostridium perfringens BEC/CPILE-Positive, Toxinotype D and E Strains Isolated from Healthy Children Toxins doi: 10.3390/toxins11090543 Authors: Raymond Kiu Kathleen Sim Alex Shaw Emma Cornwell Derek Pickard J. Simon Kroll Lindsay J. Hall Clostridium perfringens toxinotype D, toxinotype E, and gastroenteritis-linked BEC/CPILE-...
Tue, 09/17/2019 - 10:58
Abstract Clostridium perfringens is the main cause of sudden death in dogs and currently there is no vaccine to prevent it. In this study, a canine C. perfringens type A strain was used to prepare a vaccine. C. perfringens was inactivated by formaldehyde and adjuvants were added. The safety and immunological characteristics of the inactivated C. perfringens vaccine were evaluated in mice and dogs...
Mon, 09/16/2019 - 01:05
Available online 14 September 2019 Author(s): Victoria Stadler Tasca Ribeiro, Letícia Kraft, Paula Hansen Suss, Juliette Cieslinski, Luciana Cristina Wollmann, Felipe Francisco TuonAbstractAlthough reports of infections caused by anaerobes after tissue transplantation are uncommon, contamination of allografts may result in substantial complications. Anaerobic incubation and testing of organ...
Wed, 09/11/2019 - 01:05
Available online 9 September 2019 Author(s): Prabhat K. Talukdar, Mahfuzur R. SarkerAbstractClostridium perfringens SM101 genome encode three serine proteases (CspA, CspB, and CspC), and genetic evidence indicates that CspB is required for processing of pro-SleC into active SleC, an enzyme essential for degradation of the peptidoglycan cortex during spore germination. In this study, the...
Wed, 09/11/2019 - 00:00
Toxins, Vol. 11, Pages 525: Vaccine Production to Protect Animals Against Pathogenic Clostridia Toxins doi: 10.3390/toxins11090525 Authors: Zaragoza Orellana Moonen Moutafis Marcellin Clostridium is a broad genus of anaerobic, spore-forming, rod-shaped, Gram-positive bacteria that can be found in different environments all around the world. The genus includes human and animal...
Tue, 09/03/2019 - 12:01
The human pathogenic bacterium Clostridium perfringens secretes an enterotoxin (CpE) that targets claudins through its C-terminal receptor-binding domain (cCpE). Isoform-specific binding by CpE causes dissociation of claudins and tight junctions (TJs), resulting in cytotoxicity and breakdown of the gut epithelial barrier. Here, we present crystal structures of human claudin-9 (hCLDN-9)...
Fri, 08/30/2019 - 00:00
Toxins, Vol. 11, Pages 509: Granulocyte Colony-Stimulating Factor Does Not Influence Clostridium Perfringens α-Toxin-Induced Myonecrosis in Mice Toxins doi: 10.3390/toxins11090509 Authors: Masaya Takehara Yuuta Sonobe Hiroto Bandou Keiko Kobayashi Masahiro Nagahama Clostridium perfringens type A causes gas gangrene characterized by myonecrosis and development of an effective...
Thu, 08/22/2019 - 01:03
Available online 14 August 2019 Author(s): E. Gkogka, M.W. Reij, L.G.M. Gorris, M.H. ZwieteringAbstractTo assess the risk of C. perfringens toxico-infections related to the consumption of Cornish pasties in the United Kingdom, a stochastic risk assessment model was created. Exposure to the pathogen was modelled as the result of contamination of different ingredients (i.e. beef and pepper),...
Tue, 08/13/2019 - 00:00
Toxins, Vol. 11, Pages 472: Clostridium botulinum and Clostridium perfringens Occurrence in Kazakh Honey Samples Toxins doi: 10.3390/toxins11080472 Authors: Balgabay Maikanov Raikhan Mustafina Laura Auteleyeva Jan Wiśniewski Krzysztof Anusz Tomasz Grenda Krzysztof Kwiatek Magdalena Goldsztejn Magdalena Grabczak The aim of this study was to assess occurrence of Clostridium...
Fri, 08/02/2019 - 01:03
Available online 1 August 2019 Author(s): Lihan Huang, Changcheng LiAbstractThe objective of this study was to determine the kinetic parameters and apply Markov Chain Monte Carlo (MCMC) simulation to predict the growth of Clostridium perfringens from spores in ground chicken meat during dynamic cooling. Inoculated samples were exposed to various cooling conditions to observe dynamic growth. A...
Tue, 07/23/2019 - 11:00
Gas gangrene, or clostridial myonecrosis, is usually caused by Clostridium perfringens and may occur spontaneously in association with diabetes mellitus, peripheral vascular disease, or some malignancies but more often after contamination of a deep surgical or traumatic lesion. If not controlled, clostridial myonecrosis results in multiorgan failure, shock, and death, but very little is known...
Wed, 07/17/2019 - 00:00
Toxins, Vol. 11, Pages 419: Perfringolysin O-Induced Plasma Membrane Pores Trigger Actomyosin Remodeling and Endoplasmic Reticulum Redistribution Toxins doi: 10.3390/toxins11070419 Authors: Cláudia Brito Francisco S. Mesquita Christopher K. E. Bleck James R. Sellers Didier Cabanes Sandra Sousa Clostridium perfringens produces an arsenal of toxins that act together to cause...

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