Clostridium perfringens

Thu, 10/15/2020 - 06:23
Author(s): Dongryeoul Bae, Jeong-Woo Lee, Jong-Pyo Chae, Jae-Won Kim, Jong-Su Eun, Kyung-Woo Lee, Kun-Ho Seo High necrotic enteritis (NE) incidence and mortality rates in poultry can be caused by Clostridium perfringens (CP) co-infected with Eimeria spp., a causative agent of coccidiosis. Banning of prophylactic use of antibiotics in feed has been accompanied by increased NE outbreaks, resulting...
Thu, 10/15/2020 - 00:01
Clostridium perfringens poses a serious threat to small ruminants by causing moderate to severe enterotoxaemia. Due to its ability to produce a wide arsenal of toxins, it is ranked among the most prevalent and important pathogens in livestock. This study focused on the molecular characterization of different Clostridium perfringens types along with their antimicrobial resistance profile. An...
Fri, 10/09/2020 - 06:15
Author(s): Lucilla Iacumin, Giuseppe Comi Three hundred Clostridium strains were isolated from spoiled wurstels and were identified by traditional and molecular methods as Clostridium perfringens. The phenotypic characteristics of the strains were studied. All the strains produced acetic and butyric acids and enterotoxin. C. perfringens grew in the spoiled wurstels because it was present in raw...
Fri, 10/09/2020 - 00:00
Clostridium (C.) perfringens is the causative agent of several diseases and enteric infections in animals and humans. The pathogenicity of the bacterium is largely mediated by the production of a wide range of toxins. Individual C. perfringens strains produce only subsets of this toxin repertoire, which permits the classification in seven toxinotypes (A–G). In addition, a variety of...
Thu, 10/08/2020 - 06:23
Author(s): Xinheng Zhang, Qiqi Zhao, Xiaotong Ci, Sheng Chen, Zi Xie, Hongxin Li, Huanmin Zhang, Feng Chen, Qingmei Xie The gole of the study was testing the effects of chlorogenic acid (CA) supplementation on small intestine healthiness, growth performance, oxidative stress, inflammatory response and blood biochemical indices in SPF chickens after infection with Clostridium perfringens (CP) type...
Sat, 09/26/2020 - 06:23
Author(s): Jingjing Sun, Cheng Zhang, Bingkun Zhang This study evaluates the effects of Zinc amino acid complex on broiler’s intestinal permeability and integrity challenged with Clostridium perfringens (C. perfringens). A total of 180 Arbor Acres one-day-old male broilers were assigned to six treatments in a completely randomized 2 × 3 factorial design. The experiment investigated the...
Thu, 09/03/2020 - 22:16
ABSTRACT Aims This study aimed to determine the toxin genotypes, virulence determinants, and antibiogram of Clostridium perfringens isolated from poultry, animals, and humans. Biofilm formation and the efficacy of disinfectants on C. perfringens biofilms were studied. Methods and Results Thirty C. perfringens isolates (20 clinical and 10 from chicken carcasses) were genotyped by PCR and all...
Mon, 08/24/2020 - 17:38
Timely and accurate methods for detecting Clostridium perfringens-associated diseases (CPAD) are crucial to improve patient care. A number of studies have evaluated the accuracy of nucleic acid amplification tests (NAAT) in detecting CPAD, but decisive results about their effectiveness have not been reported. We conducted a meta-analysis to evaluate the diagnostic performance of NAAT for...
Sun, 08/16/2020 - 06:12
Author(s): Baikui Wang, Altaf Hussain, Yuanhao Zhou, Zihan Zeng, Qi Wang, Peng Zou, Li Gong, Pengwei Zhao, Weifen Li Macrophages are professional phagocytic cells that play a critical role in initiating immune responses by presenting antigen and phagocytic clearance. The macrophages can be targeted for immunomodulation by beneficial microbes, such as probiotics. The aim of this study is to...
Sat, 08/08/2020 - 22:14
Aim The aim of the present study was to examine the vaccine immune response in ewes supplemented with Bacillus toyonensis BCT‐7112T during a period of 5‐day supplementation before vaccination against a recombinant Clostridium perfringens epsilon toxin (rETX). Methods and Results Ewes were vaccinated with 200 µg of rETX adjuvanted with 10% aluminum hydroxide. The treat group was orally...
Tue, 07/28/2020 - 22:16
This study investigated the effects of dietary Bifidobacterium bifidum (BFD) and mannan‐oligosaccharide (MOS), as a synbiotic, on the production performance, gut microbiology, serum biochemistry, antioxidant profile, and health indices of broiler chicken. Six dietary treatments were T1 (negative control), T2 (positive control‐20 mg antibiotic BMD/kg diet; BMD: bacitracin methylene disalicylate),...
Sat, 07/25/2020 - 06:12
LWT
Author(s): Zahra H. Mohammad, Larry Payton, Sujata A. Sirsat This study aimed to investigate the efficacy of sushi rice acidification on the growth of B. cereus and C. prefringens. The white sushi rice was cooked in a rice cooker, and the pH was adjusted with rice vinegar to 4.2 ± 0.05 or 4.6 ± 0.05 to compare pH recommended by the U.S. Food Code and lower pH used by sushi retails. The rice was...
Tue, 07/21/2020 - 22:14
In this work, the fermented chili pastes of normal samples (NS) and abnormal samples (ANS, aerogenic samples) were analyzed. The gas produced in the ANS was carbon dioxide. The kinds of bacteria genus were the same in both samples. However, concentrations of Bacillus and Clostridium in ANS were higher than those in NS. This finding indicated they might be the potential bacteria for gas production...
Sun, 07/19/2020 - 06:13
Author(s): Yanhua Wang Clostridium perfringens is an important human and animal pathogen that is the primary causative agent of necrotizing enteritis and enterotoxemia in many types of animals. C. perfringens produces a variety of toxins, including NetF which may plays a crucial role in the pathogenesis of foal and canine necrotizing enteritis. In this study, we used several bioinformatics...
Tue, 07/14/2020 - 16:00
Gas gangrene, caused mainly by the anaerobic bacterium Clostridium perfringens (C. perfringens), causes death within 48 h of onset. Limited therapeutic strategies are available, and it is associated with extremely high mortality. Both C. perfringens alpha toxin (CPA) and perfringolysin O (PFO) are important virulence factors in the development of gas gangrene, suggesting that they are therapeutic...
Sat, 07/11/2020 - 04:02
In Japan, strategies for ensuring food safety have been developed without reliable scientific evidence on the relation between foodborne infections and foods. The aim of this research was to provide information on the proportions of foodborne diseases caused by seven major causative pathogens ( Campylobacte r spp., Salmonella spp., EHEC, V. parahaemolyticus , Clostridium perfringens ,...
Thu, 07/09/2020 - 06:13
Author(s): Li Xiu, Yu Liu, Wei Wu, Suo Chen, Zhaobing Zhong, Hairong Wang Clostridium perfringens is an important zoonotic microorganism, the current study was undertaken to investigate prevalence, serotype distribution, antibiotic resistance and genetic diversity of C. perfringens isolates from four duck farms in Shandong, China. In total, 424 samples of cloacal swabs and environment were...
Fri, 06/19/2020 - 04:03
The Florida Complaint and Outbreak Reporting System (FL-CORS) database is used by the Florida Department of Health’s Food and Waterborne Disease Program (FWDP) as one of the tools to detect foodborne disease outbreaks. We present a descriptive and spatial network analysis of the FL-CORS data collected during 2015-2018. We also quantified foodborne disease outbreaks (FBO) which were investigated...
Thu, 06/18/2020 - 10:02
ABSTRACT Clostridium perfringens type A is involved in gas gangrene in humans and animals. Following a traumatic injury, rapid bacterial proliferation and exotoxin production result in severe myonecrosis. C. perfringens alpha toxin (CPA) and perfringolysin (PFO) are the main virulence factors responsible for the disease. Recent in vitro studies have identified an Agr-like quorum-sensing (QS)...
Fri, 06/12/2020 - 22:15
Bacterial toxins are food safety hazards causing about 10% of all reported foodborne outbreaks in Europe. Pertinent to Gram‐positive pathogens, the most relevant toxins are emetic toxin and diarrheal enterotoxins of Bacillus cereus, neurotoxins of Clostridium botulinum, enterotoxin of Clostridium perfringens, and a family of enterotoxins produced by Staphylococcus aureus and some other...
Thu, 05/21/2020 - 03:43
To identify the free‐living amoeba (FLA) and amoeba resistant bacteria (ARB) accumulated in zebra mussels and in the water in which they are found, mussels were collected at two locations in the Ebro river basin (North East Spain). Free‐living amoebas and bacteria were isolated from mussel extracts and from natural water. PCR techniques were used to identify the FLAs and endosymbiont bacteria (...
Fri, 05/15/2020 - 03:46
Here, we report the complete genome sequence of Clostridium perfringens 2016TE7641_69, isolated from the intestine of a turkey reared in a conventional poultry flock located in central Italy, where animals were showing enteric disorders suggesting subclinical necrotic enteritis.
Fri, 05/08/2020 - 03:40
This study utilized a chicken model of chronic physiological stress mediated by corticosterone (CORT) administration to ascertain how various host metrics are altered upon challenge with Clostridium perfringens. ...
Fri, 05/01/2020 - 03:35
Epsilon-toxin produced by Clostridium perfringens significantly contributes to the pathogeneses of enterotoxemia in ruminants and multiple sclerosis in humans. Epsilon-toxin forms a heptameric oligomer in the host cell membrane, promoting cell disruption. Here, we investigate the effect of epsilon-toxin on epithelial barrier functions. Epsilon-toxin impairs the barrier integrity of Madin-Darby...
Wed, 04/08/2020 - 03:32
Author(s): Gunaranjan Paturi, Christine A. Butts, Duncan Hedderley, Halina Stoklosinski, Sheridan Martell, Hannah Dinnan, Elizabeth A. Carpenter The influence of goat and cow milk powders individually and in combination with prebiotics (inulin and oligofructose) on gut microbial populations, organic acid concentrations and gut morphology were studied in newly weaned rats fed for 21 days. The...
Wed, 04/08/2020 - 03:32
Author(s): Gunaranjan Paturi, Christine A. Butts, Duncan Hedderley, Halina Stoklosinski, Sheridan Martell, Hannah Dinnan, Elizabeth A. Carpenter The influence of goat and cow milk powders individually and in combination with prebiotics (inulin and oligofructose) on gut microbial populations, organic acid concentrations and gut morphology were studied in newly weaned rats fed for 21 days. The...
Wed, 04/08/2020 - 03:32
Author(s): Gunaranjan Paturi, Christine A. Butts, Duncan Hedderley, Halina Stoklosinski, Sheridan Martell, Hannah Dinnan, Elizabeth A. Carpenter The influence of goat and cow milk powders individually and in combination with prebiotics (inulin and oligofructose) on gut microbial populations, organic acid concentrations and gut morphology were studied in newly weaned rats fed for 21 days. The...
Fri, 04/03/2020 - 03:28
Despite the long-term interest in solventogenic clostridia-based ABE (acetone-butanol-ethanol) fermentation, clostridial butanol tolerance and its underlying mechanism remain poorly understood, which is a major obstacle hindering further improvements of this important fermentative process. In this study, a two-component system (TCS), BtrK/BtrR, was identified and demonstrated to positively...
Thu, 03/19/2020 - 03:31
Author(s): R. Shanmugasundaram, A. Markazi, M. Mortada, T.T. Ng, T.J. Applegate, L.R. Bielke, B. Syed, C.M. Pender, S. Curry, G.R. Murugesan, R.K. Selvaraj Studies were conducted to determine the efficacy of synbiotic applications to combat the negative effects of necrotic enteritis (NE). An in vitro study was conducted to test the effect of probiotics species supernatants to decrease Clostridium...
Sun, 03/01/2020 - 03:33
Author(s): Mahmoud Fayez, Ibrahim Elsohaby, Theeb Al-Marri, Kamal Zidan, Ali Aldoweriej, Elham El-Sergany, Ahmed ElmoslemanyThe present study aimed to isolate and genotype C. perfringens from healthy and diarrheic dromedary camels, pastures and herders; and to evaluate and compare antimicrobial susceptibility of the isolates. A total of 262 (56.3%) C. perfringens isolates were recovered from 465...
Sun, 02/23/2020 - 03:35
Author(s): M. Hilliar, C. Keerqin, C.K. Girish, R. Barekatain, S.-B. Wu, R.A. SwickAbstractNecrotic enteritis (NE) is an infection of the gastrointestinal tract and is estimated to cost the global poultry industry billions of dollars annually. A study was conducted to examine whether reducing the crude protein might offset the severity of NE in broilers experimentally challenged with Eimeria spp...
Sat, 02/15/2020 - 03:36
Author(s): Ryunkyoung Oh, Min Jeong Lee, Young-Ok Kim, Bo-Hye Nam, Hee Jeong Kong, Ju-Won Kim, Jung-yeon Park, Jung-Kil Seo, Dong-Gyun KimAbstractWe isolated and purified an antimicrobial peptide (AMP) from the mantle of the hard-shelled mussel, Mytilus coruscus. The peptide was purified through C18 reversed-phase high-performance liquid chromatography, and displayed antibacterial activity. Total...
Wed, 02/12/2020 - 10:49
Author(s): Uraisha Ramlucken, Santosh O. Ramchuran, Ghaneshree Moonsamy, Rajesh Lalloo, Mapitsi S. Thantsha, Christine Jansen van RensburgABSTRACTThere is a necessity for the implementation of in-feed probiotics in the poultry production industry, following strict regulations around the use of antibiotic growth promoters (AGP). Bacillus spp. are becoming an attractive alternative because of their...
Wed, 02/12/2020 - 10:49
Author(s): H.K. Zanu, C. Keerqin, S.K. Kheravii, N.K. Morgan, S-B. Wu, M.R. Bedford, R.A. SwickAbstractThis study investigated the influence of meat and bone meal (MBM), phytase, and antibiotics (AB) on the performance, intestinal pH, ileal digestibility, cecal microbiota, and tibial mineralization in Ross 308 broilers challenged with necrotic enteritis (NE). A total of 672-day-old male Ross 308...
Fri, 02/07/2020 - 03:53
Aims The purpose of this study was to isolate Lactobacillus from gastrointestinal tract of healthy post‐weaning piglets and investigate its synergistic antimicrobial and probiotic effects with ZnO nanoparticles (nZnO). Methods and Results Out of the 128 isolates, L. plantarum BLPL03 was selected based on its excellent acid and bile salt tolerance properties. L. plantarum BLPL03 was sensitive to β...
Fri, 01/31/2020 - 03:29
Salmonella enterica, Listeria monocytogenes, Shigella flexneri, Escherichia coli O157:H7, Vibrio parahaemolyticus, Staphylococcus aureus, Vibrio cholerae, Clostridium botulinum type A, Bacillus cereus, Clostridium perfringens Alpha toxin, and Yersinia enterocolitica are 11 common foodborne pathogens. Traditional bacterial culture methods for detecting pathogens are time‐consuming and labor‐...
Thu, 01/30/2020 - 03:36
Pathogenic microorganisms use various mechanisms to conserve energy in host tissues and environmental reservoirs. One widespread but often overlooked means of energy conservation is through the consumption or production of molecular hydrogen (H2). Here, we comprehensively review the distribution, biochemistry, and physiology of H2 metabolism in pathogens. Over 200 pathogens and pathobionts carry...
Thu, 01/30/2020 - 03:32
Author(s): Hani El Kadri, Teyfik Celen, Alaa Alaizoki, Madeleine Smith, Helen OnyeakaAbstractLow-temperature long-time (LTLT) cooking may lead to risk of potential survival of pathogenic bacteria such as Clostridium perfringens in cooked meat. In this study, the effect of LTLT cooking on C. perfringens was investigated at temperatures commonly used by caterers. Brain heart infusion broth (BHIB)...
Sat, 01/25/2020 - 03:30
LWT
Author(s): Padmanabha Reddy Velugoti, Saurabh Kumar, Lalit Kumar Bohra, Vijay K. Juneja, Harshavardhan ThippareddiAbstractInhibition of Clostridium perfringens spore germination and outgrowth by buffered calcium, potassium, and sodium citrates (Ca-Cit, K-Cit, and Na-Cit, respectively) during exponential cooling of non-cured and cured pork was evaluated. Antimicrobials were added to a base...
Fri, 01/24/2020 - 03:34
Clostridium perfringens causes severe gastrointestinal diseases, which include necrotic enteritis (NE) in chickens, a deadly disease worldwide. We report here the draft genome sequence of Clostridium perfringens strain TAMU, which was used in developing an NE chicken challenge model. This C. perfringens TAMU genome sequence will aid in advancing potential intervention strategies to reduce NE...
Sun, 01/19/2020 - 03:33
Author(s): Jackie K. Cheung, Vicki Adams, Danielle D’Souza, Meagan James, Christopher J. Day, Michael P. Jennings, Dena Lyras, Julian I. RoodAbstractClostridium perfringens is the causative agent of human clostridial myonecrosis; the major toxins involved in this disease are α-toxin and perfringolysin O. The RevSR two-component regulatory system has been shown to be involved in regulating...
Sat, 01/18/2020 - 03:28
Author(s): L. Grau-Roma, S. Blatter, C. Wenker, K. Sonja, M. Navarro, F. Uzal, H. PosthausIntroduction: Clostridium perfringens is the cause of necrotic enteritis (NE) in chickens. A similar disease has occasionally been reported in psittacines. We diagnosed several outbreaks of NE in lorikeets (Trichoglossus haematodus).
Tue, 01/14/2020 - 03:23
Clostridium perfringens (CP) is a foodborne pathogen. The bacterium can also inhabit human gut without symptoms of foodborne illness. However, the clinical symptoms of long-term inhabitation have not been known yet. Therefore, the objective of this study was to elucidate the relationship between intestinal CP and other internal organs. Phosphate-buffered saline (PBS) and CP were orally injected...
Sat, 01/11/2020 - 03:23
Clostridium perfringens is an important human and animal pathogen that is the primary causative agent of necrotizing enteritis and enterotoxemia in many types of animals; it causes traumatic gas gangrene in humans and animals and is associated with cases of food poisoning in humans. C. perfringens produces a variety of toxins as well as many enzymes, including three sialidases, NanH, NanI, and...
Fri, 01/10/2020 - 03:30
Author(s): Yaodi Zhu, Jiaye Zhang, Miaoyun Li, Lijun Zhao, Hongrong Ren, Longgnag Yan, Gaiming Zhao, Chaozhi ZhuAbstractThe Gram-positive, anaerobic, spore-forming bacterium, Clostridium perfringens (C. perfringens) causes a variety of diseases in humans and other animals. Spore germination is thought to be the first stage of infection by C. perfringens. AGFK, a mixture of l-asparagine, d-glucose...
Thu, 01/09/2020 - 04:04
Author(s): Simon Packer, Jane Day, Peter Hardman, Julia Cameron, Michael Kennedy, Jonathan Turner, Caroline Willis, Corinne Amar, Bayad Nozad, Maya GobinAbstractIn March 2018, Public Health England was alerted to an outbreak of gastrointestinal illness in persons who had eaten at a restaurant in the South West of England on Mothering Sunday. We aimed to determine the source of infection and...
Wed, 01/08/2020 - 03:37
Author(s): Zhen Jia, Yanhong Liu, Chang-An Hwang, Lihan HuangAbstractClostridium perfringens is a strictly anaerobic pathogen that requires absence of oxygen for its growth in laboratory experiments, which is usually attained by using an anaerobic chamber or anaerobic jars. However, it has been demonstrated that C. perfringens may survive for short periods of times due to its adaptive response to...
Tue, 01/07/2020 - 03:26
Epsilon toxin (ETX), produced by Clostridium perfringens types B and D, causes serious neurological disorders in animals. ETX can bind to the white matter of the brain and the oligodendrocytes, which are the cells forming the myelin sheath around neuron axons in the white matter of the central nervous system. After binding to oligodendrocytes, ETX causes demyelination in rat cerebellar slices. We...
Wed, 12/25/2019 - 03:34
ABSTRACT Clostridium perfringens type F strains cause gastrointestinal disease when they produce a pore-forming toxin named C. perfringens enterotoxin (CPE). In human enterocyte-like Caco-2 cells, low CPE concentrations cause caspase-3-dependent apoptosis, while high CPE concentrations cause necrosis. Since necrosis or apoptosis sometimes involves receptor-interacting serine/threonine-protein...
Fri, 11/22/2019 - 03:26
ABSTRACT Poultry meats can become contaminated with pathogenic bacteria through digesta leakage during processing. Reducing the bacteria load in digesta of market-aged broilers prior to processing reduces the incidence of fecal contamination at the processing plant. A lysozyme product was incorporated in a maltodextrin-based feed offered during the pre-shipping feed withdrawal period to reduce...

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