Cronobacter

Fri, 10/09/2020 - 22:16
ABSTRACT Aims The objective of this study was to evaluate the cytotoxic activity of Cronobacter strains isolated from foods (n=50) and clinical samples (n=6) in Brazil and genotype selected strains (n=18) using multi‐locus sequence typing (MLST). Methods and Results The cytotoxic activity of C. sakazakii (n=29), C. dublinensis (n=13), C. malonaticus (n=6), C. turicensis (n=6), and C. muytjensii (...
Fri, 09/25/2020 - 16:00
Cronobacter species are opportunistic pathogens capable of causing life-threatening infections in humans, with serious complications arising in neonates, infants, immuno-compromised individuals, and elderly adults. The genus is comprised of seven species: Cronobacter sakazakii, Cronobacter malonaticus, Cronobacter turicensis, Cronobacter muytjensii, Cronobacter dublinensis, Cronobacter...
Fri, 09/18/2020 - 06:15
Author(s): Heather Craven, Catherine McAuley, Murray Hannah, Lesley Duffy, Narelle Fegan, Stephen Forsythe The emergence of Cronobacter as an important potential pathogen for new born children and its occurrence in powdered infant formulae has generated a need to develop new management practices for this food group. This includes reduction of the prevalence of Cronobacter in manufacturing...
Sun, 09/13/2020 - 02:05
The study reports protective role of potential probiotic cultures against infection by biofilm forming Cronobacter sakazakii in Caenorhabditis elegans model system. Among the fifteen indigenous potential probiotics, the cell free supernatant of Lactobacillus gastricus BTM7 possessed highest antimicrobial action and biofilm inhibition against C. sakazakii. The competitive exclusion assays revealed...
Wed, 09/09/2020 - 06:14
Author(s): Chengsi Li, Haiyan Zeng, Jumei Zhang, Dandan Luo, Moutong Chen, Tao Lei, Xiaojuan Yang, Haoming Wu, Shuzhen Cai, Yingwang Ye, Yu Ding, Juan Wang, Qingping Wu Cronobacter species (Cronobacter spp.) are important foodborne pathogens that can infect and cause serious life-threatening diseases in infants and immunocompromised elderly. This study aimed to acquire data on Cronobacter spp....
Tue, 09/08/2020 - 06:29
Author(s): Gabriela Guimarães Carvalho, Aline Parolin Calarga, Josie Roberta Teodoro, Murilo Mariz Queiroz, Carlos A. Astudillo-Trujillo, Carlos Emilio Levy, Marcelo Brocchi, Dirce Yorika Kabuki Cronobacter spp. are opportunistic pathogens that cause serious infections, especially in infants, elderly, and immunocompromised people. Dehydrated infant foods are the main vehicle associated with...
Thu, 08/27/2020 - 08:05
The purpose of this study was to elucidate the antibacterial activity and possible mechanism of action of Amaranthus tricolor crude extract (ATCE) against Cronobacter sakazakii isolated from powdered infant formula (PIF). The antibacterial activity of ATCE was assessed by measuring the diameter of inhibition zone (DIZ), minimum inhibitory concentration (MIC), and minimum bactericidal...
Wed, 08/26/2020 - 06:17
Author(s): Chunxia Li, Xiaodong Sun, Bing Niu, Yuan Jiang, Jielin Yang, Qin Chen Cronobacter spp. is a conditioned pathogen found mainly in milk powder; it can seriously harm infant health. We investigated biofilm formation in eight strains of Cronobacter spp. and found that DSM 18707 had the strongest capacity to form biofilm. Biofilm-related genes bcsA, bcsG, flgJ, and flhE were selected for...
Fri, 08/07/2020 - 06:13
Author(s): Jingfeng Zhang, Li Wang, Lei Shi, Xun Chen, Chuxin Chen, Zicheng Hong, Yong Cao, Lichao Zhao In a viable but nonculturable (VBNC) state, bacteria are no longer culturable on standard laboratory media, but still, remain a pathogenic potential and present possible health risks. In this study, we investigated ampicillin's ability, which is commonly used in dairy cattle disease treatment,...
Tue, 08/04/2020 - 22:18
This study investigated antimicrobial efficacy of trans‐cinnamaldehyde (TC) and chitosan (CH) along with high pressure processing (HPP) against Cronobacter sakazakii in infant formula. All treatments exhibited varying degrees of C. sakazakii reductions up to undetectable levels. Remarkable deformation and damage in C. sakazakii cells were observed by transmission electron microscopy after the...
Sat, 07/25/2020 - 22:15
Lateral‐flow immunochromatographic assay (LFIA) was performed on pastry samples incubated for different time‐periods and the degree of coliform contamination evaluated. Pastries contaminated with ~5.0 log10 (cfu/g) coliforms showed LFIA positivity by 3 hr culture, whereas pastries contaminated with 2.0 log10 (cfu/g) coliforms showed LFIA positivity by 9 hr culture. The degree of coliform...
Sun, 06/28/2020 - 06:11
Author(s): Dongjie Chen, Justin R. Wiertzema, Peng Peng, Yanling Cheng, Yunpu Wang, Juer Liu, Yiwei Ma, Wes Mosher, Myungwoo Kang, Min Min, Paul Chen, David J. Baumler, Chi Chen, Laurence Lee, Zata Vickers, Joellen Feirtag, Roger Ruan The outbreaks of Cronobacter sakazakii, Salmonella spp, and Bacillus cereus in powdered foods have been increasing in worldwide. However, an effective method to...
Wed, 06/17/2020 - 16:00
Cronobacter sakazakii is an opportunistic Gram-negative pathogen that could cause meningitis and necrotizing enterocolitis. Several Gram-negative bacteria use the PmrA/PmrB system to sense and adapt to environmental change by resistance to cationic antimicrobial peptides of host immune systems. The PmrA/PmrB two-component system regulates several genes to modify LPS structure in the bacterial...
Wed, 06/10/2020 - 22:17
Two medicinal plants, African Locust Bean (Parkia biglobosa) and Lonicera japonica, traditionally used in West Africa and China were investigated against four spoilage microorganisms: Bacillus subtilis, Cronobacter dublinensis, Pantoea agglomerans, and Bacillus sp., isolated from mulberry fruit. The aqueous extract of the P. biglobosa (ALB) was potent with average inhibitory zones against four...
Tue, 06/09/2020 - 16:00
Cronobacter sakazakii is foodborne pathogen that causes serious illnesses such as necrotizing enterocolitis, meningitis and septicemia in infants. However, the virulence determinants and mechanisms of pathogenicity of these species remain unclear. In this study, multilocus sequence typing (MLST) was performed on 34 C. sakazakii strains and two strains with the same sequence type (ST) but distinct...
Sun, 06/07/2020 - 06:11
Author(s): Rajni Chauhan, Niharika Singh, Gaurav Kumar Pal, Gunjan Goel Cronobacter sakazakii is an emerging foodborne pathogen, causing life-threatening infections in newborns and premature infants. Cronobacter spp. can survive under difficult processing conditions thereby contaminate the Powdered Infant Formula (PIF) during the manufacture process. Infantile infections are associated with the...
Thu, 06/04/2020 - 03:38
Due to the lack of electricity and thermostatic instruments in certain settings for convenient detection of Cronobacter species in powdered infant formula (PIF), a novel investigation was conducted to establish an electricity-free visual detection system for rapid detection of Cronobacter species in PIF. This system included a portable electricity-free heater that could use the exothermic...
Tue, 05/19/2020 - 03:49
The U.S. Food and Drug Administration (FDA) conducted a sampling assignment in 2014 to ascertain the prevalence of Cronobacter spp. and Salmonella in the processing environment of facilities manufacturing milk powder.Cronobacter was detected in the environment of 38 (69%) of 55 facilities. The average prevalence of Cronobacter in 5,671 subsamples (i.e., swabs/sponges from different facility...
Tue, 05/12/2020 - 03:43
Author(s): Tae Jin Cho, Hye Won Kim, Changhoon Yoo, Sung Woo Kim, Min Suk Rhee Microfluidic labchips have achieved much advancement in the molecular diagnosis of foodborne pathogens. Whereas difficulties in the flow control during the transportation of liquid fluids can occur and should be overcome. Manipulations of reaction temperature and the complex procedures from sample pre-treatment to...
Sat, 05/09/2020 - 03:46
Author(s): Hong Qin, Xiuquan Shi, Liang Yu, Ke Li, Jianwu Wang, Jihua Chen, Fei Yang, Haiyan Xu, Huilan Xu A sodium dodecyl sulphate (SDS)-propidium monoazide (PMA)-multiplex real-time PCR (mRT-PCR) assay for accurate and rapid detection of viable Listeria monocytogenes, Cronobacter sakazakii, Staphylococcus aureus and Salmonella spp. was established. The SDS and PMA pretreatments were used to...
Sun, 05/03/2020 - 03:39
Aim This research aimed to monitor pharmaceutical water system by sampling water from all treatment stages, identify bacterial isolates from each phase and determine the most suitable methods to control them. Methods and results Water samples were collected and examined from pharmaceutical water system in a pharmaceutical factory in Giza, Egypt during twelve months, once per month, (from December...
Fri, 04/24/2020 - 03:39
Cronobacter sakazakii (C. sakazakii) is an opportunistic, neonatal, and food borne pathogen primarily associated with the contamination of powdered infant formula (PIF). The pathogen is reported to overcome the food safety barriers such as increased acidity, heat treatment, and so on. This study evaluates the thermal tolerance of C. sakazakii strains independently at 52, 55, and 58°C in...
Thu, 04/16/2020 - 03:27
J. Strysko et al. Invasive Cronobacter infections among infants are associated with severe neurologic disabilities and death. Early Cronobacter reports typically featured hospitalized and preterm infants and recognized contaminated powdered infant formula (PIF) as a transmission vehicle. To clarify recent epidemiology, we reviewed all cases of bloodstream infection or meningitis among infants...
Wed, 04/08/2020 - 03:33
Necrotizing enterocolitis (NEC) is a serious inflammatory intestinal disorder with a high mortality rate, which occurs most commonly in newborn infants. Cronobacter sakazakii, a common contaminant in infant formula, is associated with NEC. However, its role in NEC pathogenesis is unknown, and there are still no effective treatments for NEC. Currently, natural bioactive products have been...
Mon, 04/06/2020 - 03:28
This study was aimed to evaluate the efficiency of the organic chlorine and acetic acid solutions on the inactivation of adhered cells of Escherichia coli, Cronobacter sakazakii and Klebsiella pneumoniae to lettuce. Besides, the growth and inactivation of K. pneumoniae adhered to lettuce was modeled. According to the findings, the use of chlorine solution (170 mg/ml of total residual chlorine)...
Fri, 03/27/2020 - 04:00
Author(s): Yue Zhang, Yongkang Xie, Juming Tang, Shaojin Wang, Lu Wang, Guangfei Zhu, Xingyi Li, Yanhong Liu A 27.12 MHz thermostatic radio frequency (RF) system was employed to inactivate Cronobacter sakazakii in powdered infant formula milk. A proportional-integral-derivative controller achieved constant material temperature during the holding period. Hot air and similar dielectric material...
Wed, 03/11/2020 - 03:26
Salinity stress is a major challenge to agricultural productivity and global food security in light of a dramatic increase of human population and climate change. Plant growth promoting bacteria can be used as an additional solution to traditional crop breeding and genetic engineering. In the present work, the induction of plant salt tolerance by the desert plant endophyte Cronobacter sp. JZ38...
Tue, 03/10/2020 - 03:25
Background and AimCronobacter sakazakii (C. sakazakii) has attracted considerable attention as an emerging neonatal pathogen and has been associated with outbreaks of life-threatening septicemia, necrotizing enterocolitis, and meningitis in neonates and infants globally. No data about the role of C. sakazakii as a cause of neonatal sepsis in North Africa is availale. Herein, we aimed to study the...
Thu, 02/27/2020 - 03:46
In this study, we established a rapid, simple, and sensitive method for visual and point-of-care detection of Salmonella spp., Cronobacter spp., and Staphylococcus aureus in powdered infant formula (PIF) based on multiplex loop-mediated isothermal amplification (mLAMP) combined with lateral flow dipstick (LFD). Three different species-specific target genes, siiA of Salmonella spp., internal...
Sat, 02/08/2020 - 03:25
Cronobacter spp. can cause systemic infections, such as meningitis, sepsis, and necrotizing enterocolitis, in immunocompromised patients, especially neonates. Although some virulence factors have been reported previously, the pathogenesis of Cronobacter remains unclear. In this study, we compared genome sequences from different Cronobacter species, sequence types, and sources, with the virulence...
Fri, 02/07/2020 - 03:47
Cronobacter spp. are important opportunistic foodborne pathogens in powdered infant formula that cause many serious diseases in neonates and infants. In this study, a novel assay based on dual signal amplification strategy was developed by coupling asymmetric tailing PCR (AT-PCR) with rolling circle amplification (RCA) for the detection of Cronobacter spp. in milk. The tailing single-stranded DNA...
Thu, 02/06/2020 - 03:34
Author(s): Haiyan Zeng, Chengsi Li, Na Ling, Jumei Zhang, Moutong Chen, Tao Lei, Shi Wu, Xiaojuan Yang, Dandan Luo, Yu Ding, Juan Wang, Shuhong Zhang, Qingping WuAbstractCronobacter spp. are important foodborne pathogens that infections occur in all age groups, especially cause serious life-threatening diseases in infants. This study aimed to acquire data on Cronobacter spp. contamination of meat...
Sat, 02/01/2020 - 03:42
Cronobacter sakazakii is a food-borne pathogen carried in milk powder that can cause severe bacteremia, enterocolitis, and meningitis in newborns, which can lead to death of newborns. Preventing infection by this pathogen is significant to the health of newborns. Since infants and young children are the main target group of C. sakazakii, it is considered that maternal immunity can enhance the...
Fri, 01/24/2020 - 03:31
Author(s): Yi Li, Leyi Zhang, Yuqin Hu, Chengji Hong, Airong Xie, Yuejin Wu, Zhihui, Shangguan, Lingling Mei, Biao Zhou, Yanjun Zhang, Lei FangAbstractPathogenic Cronobacter species are responsible for life-threatening illness in neonates. A ten-year comprehensive survey was conducted to examine the population structure and antimicrobial resistant patterns of Cronobacter isolates from food (n=78...
Mon, 01/13/2020 - 03:28
LWT
Author(s): Yifang Cao, Ailian Zhou, Donggen Zhou, Xinglong Xiao, Yigang Yu, Xiaofeng LiAbstractResponse mechanism of Cronobacter sakazakii under stress of combination of citral and carvacrol was investigated by transcriptome sequencing. Twenty-five significantly differentially expressed genes were identified to be closely related to the ability of C. sakazakii tolerating the stress after analysis...
Sun, 01/12/2020 - 03:30
Author(s): Song Zhang, Jian Xiong, Wenyong Lou, Zhengxiang Ning, Denghui Zhang, Jiguo YangAbstractCronobacter sakazakii (C. sakazakii) is an opportunistic foodborne pathogen in infant formula. This study was designed to explore the inhibitory effect of TGML on C. sakazakii in reconstituted infant formula (RIF). Firstly, the growth curve of C. sakazakii in RIF treated by TGML and the effect of...
Tue, 12/31/2019 - 04:08
Author(s): Ailian Zhou, Yifang Cao, Donggen Zhou, Shuangfang Hu, Wanjing Tan, Xinglong Xiao, Yigang Yu, Xiaofeng LiAbstractCronobacter sakazakii is a common foodborne pathogen that can tolerate various stress conditions. Acidic environment is a common stress condition encountered by bacteria in food processing and gastrointestinal digestion, including both inorganic and organic acids. In order to...
Sun, 12/29/2019 - 03:33
Author(s): Saidou Harouna, Indira Franco, Juan J. Carramiñana, Arturo Blázquez, Inés Abad, María D. Pérez, Miguel Calvo, Lourdes SánchezAbstractBovine lactoferrin (bLF) is an iron-binding glycoprotein used in functional and therapeutic products due to its biological properties, the most important being its antimicrobial activity. In this study, hydrolysates of bovine lactoferrin (bLFH) obtained...
Sun, 12/29/2019 - 03:26
Cronobacter sakazakii is an emerging opportunistic bacterial pathogen known to cause neonatal and pediatric infections, including meningitis, necrotizing enterocolitis, and bacteremia. Multiple disease outbreaks ...
Tue, 12/24/2019 - 03:28
Enzymatic treatment is capable of removing the biofilm matrix so that the subsequent disinfection can be more effective. Abstract Bacterial biofilms are highly difficult to control, hence significant economic resources have been allocated to develop strategies to eradicate them. This study evaluated the effect of an enzymatic treatment to be used as a cleaning product to control the presence of...
Fri, 11/22/2019 - 03:36
Author(s): Paula Vasconcelos Costa, Luiza Vasconcellos, Ingrid Camelo da Silva, Valéria de Mello Medeiros, Stephen James Forsythe, Marcelo Luiz Lima BrandãoAbstractThe aim of this study was to evaluate the microbiological quality of 45 samples of corn-based farinaceous foods commercialized in Brazil. The bacteriological analysis performed were: detection of Salmonella and Cronobacter, and...
Fri, 11/15/2019 - 03:36
Cronobacter sakazakii is an emerging pathogen that causes meningitis, bacteremia, sepsis, and necrotizing enterocolitis in premature infants. Strain Cr268 was isolated from imported powdered infant formula in 2009 during routine microbial examination according to ISO-22964 ("Microbiology of the food chain—horizontal method for the detection of Cronobacter spp."). Isolate Cr268 was confirmed...
Thu, 11/07/2019 - 03:29
The microbial population of kefir made from Korean kefir grain was profiled via next‐generation sequencing technology at the species level. Interestingly, Acetobacter fabarum was the second most predominent bacteria in kefir. We isolated Acetobacter fabarum DH1801 strain and revealed its potent and broad‐spectrum antimicrobial activity against pathogenic bacteria. This could contribute to the...
Tue, 10/22/2019 - 03:39
Blue light (BL) exerts an antimicrobial effect on pathogenic bacteria. It has been hypothesized that its bactericidal activity depends upon the generation of reactive oxygen species (such as anion superoxides) and the resultant cellular damage. However, some aspects of this hypothesis needed to be tested and investigated. Thus, the work conducted herein examined the molecular impact of BL...
Sun, 10/20/2019 - 03:42
Author(s): Ömer Akineden, Tobias Wittwer, Katrin Geister, Madeleine Plötz, Ewald UsleberAbstractA novel nucleic acid lateral flow immunoassay (NALFIA) system for Cronobacter (C.) sakazakii and C. malonaticus is based on amplification of the rpoB gene in the presence of a heterobifunctional, Cronobacter-specific DNA probe. Partial degradation of this probe during amplification is then detected by...
Sat, 10/19/2019 - 03:42
Author(s): Yifei Chen, Qiwu Wen, Shan Chen, Du Guo, Yunfeng Xu, Sen Liang, Xiaodong Xia, Baowei Yang, Chao ShiAbstractCronobacter sakazakii is a foodborne bacterial pathogen with resistance to a wide range of stress conditions. Here, we investigated the effects of thymoquinone (TQ) on the environmental and antibiotic stress tolerance of C. sakazakii. After determining the subinhibitory...
Fri, 10/18/2019 - 03:32
Cronobacter infections of infants are commonly regarded as due to the ingestion of contaminated feed. The aim of this study was to determine the occurrence of Cronobacter, total coliforms and Escherichia coli in different brands of natural mineral waters as sold in 20L returnable bottles in Rio de Janeiro, Brazil. The quantification of total coliforms and E. coli was performed by Most Probable...
Tue, 10/15/2019 - 03:37
Aims This study was aimed at revealing the composition of microbiota in leaves, roots and rhizosphere soil of wheat plants that are resistant or susceptible to stripe rust, one of the most widely destructive leaf diseases in wheat production. Methods and Results A total of 36 wheat plants that showed resistant or susceptible reactions to stripe rust were sampled. Three compartments of each plant...
Wed, 10/09/2019 - 03:38
Author(s): Zhibin Liu, Zhiyao Wang, Jinyuan Sun, Li NiAbstractGutian Hong Qu glutinous rice wine (GHQW) is a representative alcoholic beverage in China. During its traditional brewing process, multiple strains are involved. However, the roles of these microorganisms, particularly their contributions to aroma formation, are poorly understood. Therefore, in this study, the dynamics of volatile...
Tue, 09/24/2019 - 03:38
Author(s): Du Guo, Shuo Wang, Jiahui Li, Fangting Bai, Yanpeng Yang, Yunfeng Xu, Sen Liang, Xiaodong Xia, Xin Wang, Chao ShiAbstractCoenzyme Q0 (CoQ0) has demonstrated antitumor, anti-inflammatory, and anti-angiogenic activities. Cronobacter sakazakii is an opportunistic foodborne pathogen associated with high mortality in neonates. In this study, the antimicrobial activity and possible...

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