Cronobacter

Sat, 02/08/2020 - 03:25
Cronobacter spp. can cause systemic infections, such as meningitis, sepsis, and necrotizing enterocolitis, in immunocompromised patients, especially neonates. Although some virulence factors have been reported previously, the pathogenesis of Cronobacter remains unclear. In this study, we compared genome sequences from different Cronobacter species, sequence types, and sources, with the virulence...
Fri, 02/07/2020 - 03:47
Cronobacter spp. are important opportunistic foodborne pathogens in powdered infant formula that cause many serious diseases in neonates and infants. In this study, a novel assay based on dual signal amplification strategy was developed by coupling asymmetric tailing PCR (AT-PCR) with rolling circle amplification (RCA) for the detection of Cronobacter spp. in milk. The tailing single-stranded DNA...
Thu, 02/06/2020 - 03:34
Author(s): Haiyan Zeng, Chengsi Li, Na Ling, Jumei Zhang, Moutong Chen, Tao Lei, Shi Wu, Xiaojuan Yang, Dandan Luo, Yu Ding, Juan Wang, Shuhong Zhang, Qingping WuAbstractCronobacter spp. are important foodborne pathogens that infections occur in all age groups, especially cause serious life-threatening diseases in infants. This study aimed to acquire data on Cronobacter spp. contamination of meat...
Sat, 02/01/2020 - 03:42
Cronobacter sakazakii is a food-borne pathogen carried in milk powder that can cause severe bacteremia, enterocolitis, and meningitis in newborns, which can lead to death of newborns. Preventing infection by this pathogen is significant to the health of newborns. Since infants and young children are the main target group of C. sakazakii, it is considered that maternal immunity can enhance the...
Fri, 01/24/2020 - 03:31
Author(s): Yi Li, Leyi Zhang, Yuqin Hu, Chengji Hong, Airong Xie, Yuejin Wu, Zhihui, Shangguan, Lingling Mei, Biao Zhou, Yanjun Zhang, Lei FangAbstractPathogenic Cronobacter species are responsible for life-threatening illness in neonates. A ten-year comprehensive survey was conducted to examine the population structure and antimicrobial resistant patterns of Cronobacter isolates from food (n=78...
Mon, 01/13/2020 - 03:28
LWT
Author(s): Yifang Cao, Ailian Zhou, Donggen Zhou, Xinglong Xiao, Yigang Yu, Xiaofeng LiAbstractResponse mechanism of Cronobacter sakazakii under stress of combination of citral and carvacrol was investigated by transcriptome sequencing. Twenty-five significantly differentially expressed genes were identified to be closely related to the ability of C. sakazakii tolerating the stress after analysis...
Sun, 01/12/2020 - 03:30
Author(s): Song Zhang, Jian Xiong, Wenyong Lou, Zhengxiang Ning, Denghui Zhang, Jiguo YangAbstractCronobacter sakazakii (C. sakazakii) is an opportunistic foodborne pathogen in infant formula. This study was designed to explore the inhibitory effect of TGML on C. sakazakii in reconstituted infant formula (RIF). Firstly, the growth curve of C. sakazakii in RIF treated by TGML and the effect of...
Tue, 12/31/2019 - 04:08
Author(s): Ailian Zhou, Yifang Cao, Donggen Zhou, Shuangfang Hu, Wanjing Tan, Xinglong Xiao, Yigang Yu, Xiaofeng LiAbstractCronobacter sakazakii is a common foodborne pathogen that can tolerate various stress conditions. Acidic environment is a common stress condition encountered by bacteria in food processing and gastrointestinal digestion, including both inorganic and organic acids. In order to...
Sun, 12/29/2019 - 03:33
Author(s): Saidou Harouna, Indira Franco, Juan J. Carramiñana, Arturo Blázquez, Inés Abad, María D. Pérez, Miguel Calvo, Lourdes SánchezAbstractBovine lactoferrin (bLF) is an iron-binding glycoprotein used in functional and therapeutic products due to its biological properties, the most important being its antimicrobial activity. In this study, hydrolysates of bovine lactoferrin (bLFH) obtained...
Sun, 12/29/2019 - 03:26
Cronobacter sakazakii is an emerging opportunistic bacterial pathogen known to cause neonatal and pediatric infections, including meningitis, necrotizing enterocolitis, and bacteremia. Multiple disease outbreaks ...
Tue, 12/24/2019 - 03:28
Enzymatic treatment is capable of removing the biofilm matrix so that the subsequent disinfection can be more effective. Abstract Bacterial biofilms are highly difficult to control, hence significant economic resources have been allocated to develop strategies to eradicate them. This study evaluated the effect of an enzymatic treatment to be used as a cleaning product to control the presence of...
Fri, 11/22/2019 - 03:36
Author(s): Paula Vasconcelos Costa, Luiza Vasconcellos, Ingrid Camelo da Silva, Valéria de Mello Medeiros, Stephen James Forsythe, Marcelo Luiz Lima BrandãoAbstractThe aim of this study was to evaluate the microbiological quality of 45 samples of corn-based farinaceous foods commercialized in Brazil. The bacteriological analysis performed were: detection of Salmonella and Cronobacter, and...
Fri, 11/15/2019 - 03:36
Cronobacter sakazakii is an emerging pathogen that causes meningitis, bacteremia, sepsis, and necrotizing enterocolitis in premature infants. Strain Cr268 was isolated from imported powdered infant formula in 2009 during routine microbial examination according to ISO-22964 ("Microbiology of the food chain—horizontal method for the detection of Cronobacter spp."). Isolate Cr268 was confirmed...
Thu, 11/07/2019 - 03:29
The microbial population of kefir made from Korean kefir grain was profiled via next‐generation sequencing technology at the species level. Interestingly, Acetobacter fabarum was the second most predominent bacteria in kefir. We isolated Acetobacter fabarum DH1801 strain and revealed its potent and broad‐spectrum antimicrobial activity against pathogenic bacteria. This could contribute to the...
Tue, 10/22/2019 - 03:39
Blue light (BL) exerts an antimicrobial effect on pathogenic bacteria. It has been hypothesized that its bactericidal activity depends upon the generation of reactive oxygen species (such as anion superoxides) and the resultant cellular damage. However, some aspects of this hypothesis needed to be tested and investigated. Thus, the work conducted herein examined the molecular impact of BL...
Sun, 10/20/2019 - 03:42
Author(s): Ömer Akineden, Tobias Wittwer, Katrin Geister, Madeleine Plötz, Ewald UsleberAbstractA novel nucleic acid lateral flow immunoassay (NALFIA) system for Cronobacter (C.) sakazakii and C. malonaticus is based on amplification of the rpoB gene in the presence of a heterobifunctional, Cronobacter-specific DNA probe. Partial degradation of this probe during amplification is then detected by...
Sat, 10/19/2019 - 03:42
Author(s): Yifei Chen, Qiwu Wen, Shan Chen, Du Guo, Yunfeng Xu, Sen Liang, Xiaodong Xia, Baowei Yang, Chao ShiAbstractCronobacter sakazakii is a foodborne bacterial pathogen with resistance to a wide range of stress conditions. Here, we investigated the effects of thymoquinone (TQ) on the environmental and antibiotic stress tolerance of C. sakazakii. After determining the subinhibitory...
Fri, 10/18/2019 - 03:32
Cronobacter infections of infants are commonly regarded as due to the ingestion of contaminated feed. The aim of this study was to determine the occurrence of Cronobacter, total coliforms and Escherichia coli in different brands of natural mineral waters as sold in 20L returnable bottles in Rio de Janeiro, Brazil. The quantification of total coliforms and E. coli was performed by Most Probable...
Tue, 10/15/2019 - 03:37
Aims This study was aimed at revealing the composition of microbiota in leaves, roots and rhizosphere soil of wheat plants that are resistant or susceptible to stripe rust, one of the most widely destructive leaf diseases in wheat production. Methods and Results A total of 36 wheat plants that showed resistant or susceptible reactions to stripe rust were sampled. Three compartments of each plant...
Wed, 10/09/2019 - 03:38
Author(s): Zhibin Liu, Zhiyao Wang, Jinyuan Sun, Li NiAbstractGutian Hong Qu glutinous rice wine (GHQW) is a representative alcoholic beverage in China. During its traditional brewing process, multiple strains are involved. However, the roles of these microorganisms, particularly their contributions to aroma formation, are poorly understood. Therefore, in this study, the dynamics of volatile...
Tue, 09/24/2019 - 03:38
Author(s): Du Guo, Shuo Wang, Jiahui Li, Fangting Bai, Yanpeng Yang, Yunfeng Xu, Sen Liang, Xiaodong Xia, Xin Wang, Chao ShiAbstractCoenzyme Q0 (CoQ0) has demonstrated antitumor, anti-inflammatory, and anti-angiogenic activities. Cronobacter sakazakii is an opportunistic foodborne pathogen associated with high mortality in neonates. In this study, the antimicrobial activity and possible...
Thu, 09/19/2019 - 14:29
Available online 16 September 2019 Author(s): Yawen Lin, Jeyamkondan Subbiah, Long Chen, Tushar Verma, Yanhong LiuAbstractThis study validated the radio frequency assisted traditional thermal processing (RF assisted-TTP) for pasteurization of low-moisture foods. Powdered infant formula milk (PIFM) with water activity of 0.22 ± 0.01 was selected as a low-moisture food model. The cold spot during...
Wed, 09/11/2019 - 00:00
The traditional gold nanoparticle (AuNP) growth-based plasmonic ELISA (pELISA) strictly and directly controlled by reducing reagents can achieve high sensitivity, but it remains fragile toward the surrounding environment. This work developed a sandwich pELISA for Cronobacter detection in powdered infant formula samples by mediating AuNP growth through DNA. In this assay, DNA adsorbed on the...
Wed, 09/04/2019 - 03:53
The purpose of the study was to determine if pomegranate (Punica granatum L.) peel contains polyphenolic compounds with antibacterial activity against Cronobacter sakazakii. Minimum inhibitory and bactericidal concentrations of a methanolic extract were found to increase significantly (P < .05) with decreasing temperature (37, 21, 10 °C) and pH (6.0, 5.0, 4.0). Chemical analysis by high...
Tue, 08/27/2019 - 20:00
Cronobacter strains harboring CRISPR-Cas systems are important foodborne pathogens that cause serious neonatal infections. CRISPR typing is a new molecular subtyping method to track the sources of pathogenic bacterial outbreaks and shows a promise in typing Cronobacter, however, this molecular typing procedure using routine PCR method has not been established. Therefore, the purpose of this study...
Tue, 08/20/2019 - 20:00
ABSTRACT The aim of this study was to investigate the prevalence and genotypic characteristics of Cronobacter isolated from powdered infant formula (PIF) manufacturing facilities and identify the potential source of contamination. A total of 42 Cronobacter isolates (5%) were detected in 835 environmental samples collected during the surveillance study. These isolates included C. sakazakii (n=37...
Wed, 07/17/2019 - 01:03
LWT
Publication date: Available online 15 July 2019Source: LWTAuthor(s): Francesca Rita Dinardo, Fabio Minervini, Maria De Angelis, Marco Gobbetti, Michael G. GänzleAbstractThis study aimed to assess, in model sourdough fermentations, the relevance of physico-chemical features of flour for the interactions between Enterobacteriaceae and lactobacilli, and for the interactions within the latter...
Mon, 07/15/2019 - 01:05
Available online 13 July 2019 Author(s): Xiuqin Lou, Tao Liu, Wei Zhang, Hua Yu, Haoqiu Wang, Shujuan Song, Qi Chen, Zhiguo FangAbstractCronobacter was positive in cereals at a relatively high rate. In the present study, we investigated the occurrence and characteristics of this pathogen systematically in diverse cereals. All sampled food (N = 467) contained Cronobacter with a high positive rate...
Thu, 07/11/2019 - 00:00
Abstract The effect of the cell-free culture supernatants (CFCSs) from different Lacobacillus spp. on growth ability of Cronobacter sakazakii ATCC 29544 was investigated by time-killing studies. The antimicrobial effect was evaluated using crude and 2.5 × concentrated CFCSs. Most of the CFCSs showed a dose-dependent antimicrobial activity, with the greatest C. sakazakii growth...
Wed, 07/03/2019 - 13:04
Abstract In this study, we investigated the antibacterial activity of hexahydro‐β‐acids/methyl‐β‐cyclodextrin inclusion complex (HBA/M‐β‐CD) and researched its antibacterial mechanism against Staphylococcus aureus and Enterobacter sakazakii. The result showed that bacteria had different sensitivities to HBA/M‐β‐CD with diameters of inhibition zones ranging from 7.8 ± 0.5 to 14.8 ± 0.7 mm and...
Mon, 07/01/2019 - 20:00
Cronobacter sakazakii is an emerging foodborne pathogen, which is linked to life-threatening infections causing septicemia, meningitis and necrotizing enterocolitis. These infections have been epidemiologically connected to ingestion of contaminated reconstituted powder infant formula. Even at low water activity C. sakazakii can survive for a long time; it is capable of protective biofilm...
Sun, 06/30/2019 - 01:12
Available online 29 June 2019 Author(s): D. Lindsay, S. Laing, K.I. Fouhy, L. Souhoka, A.K. Beaven, T.K. Soboleva, P.K. MalakarAbstractCronobacter spp. are opportunistic pathogens that must be controlled in infant powder manufacturing plants. This study evaluated the spread of Cronobacter cells via contact surfaces within a dairy manufacturing environment. Transfer rates of Cronobacter spp. were...
Fri, 05/31/2019 - 01:12
Available online 27 May 2019 Author(s): Dongjie Chen, Yanling Cheng, Peng Peng, Juer Liu, Yunpu Wang, Yiwei Ma, Erik Anderson, Chi Chen, Paul Chen, Roger RuanAbstractCronobacter sakazakii and Salmonella spp. are foodborne pathogens associated with low moisture foods. An intense pulsed light (IPL) system is being developed as an alternative novel method to pasteurize powdered food. The aim of the...
Tue, 05/28/2019 - 03:00
Journal of Food Protection, Volume 82, Issue 6, Page 1082-1088, June 2019. Salmonella and Cronobacter are two bacteria of concern in powdered food ingredients with low water activity, due to their ability to remain viable for long periods of time. There is great interest in studying the survival of these bacteria in powdered foods, but discrepancies have been reported between broth-grown and lawn...
Thu, 05/16/2019 - 11:01
Mahewu is a fermented cereal beverage produced in Zimbabwe. This study determined the composition and origin of mahewu microbiota. The microbiota of mahewu samples consisted of 3 to 7 dominant strains of lactobacilli and two strains of yeasts. Enterobacteriaceae were not detected. Candida glabrata was present in high cell counts from samples collected in summer but not from samples collected in...
Thu, 05/02/2019 - 00:00
Cronobacter turicensis is a food-borne pathogen found in dairy products. It has been reported to cause bacteremia and enteritis in immunocompromised people, especially infants. Cronobacter turicensis has been isolated from various food sources, and contaminated powdered infant formula was found to be the most common source of infection among infants. Although some gene targets are used for the...
Tue, 04/30/2019 - 20:00
Random Mutagenesis Applied to Reveal Factors Involved in Oxidative Tolerance and Biofilm Formation in Foodborne Cronobacter malonaticus Maofeng Zhang1,2†, Xiyan Zhang1†, Liaowang Tong1†, Dexin Ou1, Yaping Wang1, Jumei Zhang2, Qingping Wu2* and Yingwang Ye1,2* 1School of Food Science and Engineering, Hefei University of Technology, Hefei, China 2State Key Laboratory of...
Sat, 04/06/2019 - 01:12
Publication date: 30 August 2019 , Volume 290Author(s): Dongjie Chen, Peng Peng, Nan Zhou, Yanling Cheng, Min Min, Yiwei Ma, Qingqing Mao, Paul Chen, Chi Chen, Roger RuanAbstractCronobacter sakazakii can cause life-threatening infections in neonates. Exposure to contaminated powdered food, especially milk powder, is a major route for C. sakazakii infection. Cold atmospheric plasma (CAP) is well...
Sun, 03/03/2019 - 01:14
LWT
Publication date: Available online 28 February 2019Source: LWTAuthor(s): Yunzhe Zhang, Qian Yang, Cong Li, Yaowu Yuan, Wei ZhangAbstractCronobacter species are opportunistic foodborne pathogens involved in neonatal infections from powdered infant formula (PIF). In this study, a novel saltatory rolling circle amplification (SRCA) method has been developed for detecting Cronobacter spp. in PIF. 16...
Tue, 02/26/2019 - 01:14
Chengsi Li, Haiyan Zeng, Jumei Zhang, Wenjing He, Na Ling, Moutong Chen, Shi Wu, Tao Lei, Haoming Wu, Yingwang Ye, Yu Ding, Juan Wang, Xianhu Wei, Youxiong Zhang, Qingping Wu Cronobacter spp. are foodborne pathogens that can infect and cause life-threatening diseases in all age groups, particularly in infants and immunocompromised elderly. This study aimed to investigate the prevalence,...
Thu, 02/21/2019 - 00:00
Escherichia coli O157:H7, Cronobacter spp., and Salmonella spp. are common food-borne pathogens in milk that may cause serious diseases. In the present study, we established a simple, rapid, and specific method to simultaneously detect viable E. coli O157:H7, Cronobacter spp., and Salmonella spp. in milk. Three specific genes, fliC from E. coli O157:H7, cgcA from Cronobacter spp., and invA from...
Wed, 02/20/2019 - 01:13
Yichen Bai, Haibo Yu, Du Guo, Shengyi Fei, Chao Shi The aim of this study was to evaluate the survival of Cronobacter sakazakii exposed to vacuum or air packaging, then stored at 4, 10, or 25°C, and the environmental stress resistance of vacuum-packaged or air-packaged bacterial cells were determined by subjecting the cells to reconstituted infant formula at 50°C, in acid (simulated gastric fluid...
Fri, 02/08/2019 - 01:12
Available online 6 February 2019 Author(s): Xinjie Song, Shruti Shukla, Myunghee KimAbstractCronobacter species are foodborne pathogens that pose a high risk in infant formula and can cause fatality rates of 40–80% in infected infants. To develop a rapid and easy detection method for Cronobacter species, especially in powdered infant formula (PIF), an immunoliposome-based immunochromatographic...
Thu, 02/07/2019 - 00:00
S. Lepuschitz et al. Cronobacter sakazakii has been documented as a cause of life-threating infections, predominantly in neonates. We conducted a multicenter study to assess the occurrence of C. sakazakii across Europe and the extent of clonality for outbreak detection. National coordinators representing 24 countries in Europe were requested to submit all human C. sakazakii isolates collected...
Thu, 01/24/2019 - 01:03
Available online 22 January 2019 Author(s): Qi Wang, Stephen James Forsythe, Xiao-Juan Zhao, Zi-Wei Wang, Dan Li, Dan Ma, Jia-Yue Cao, Jing ZengAbstractCronobacter spp. are associated with serious infections in neonates with the clinical presentations of necrotizing enterocolitis, bacteraemia and meningitis. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF...
Wed, 01/23/2019 - 11:00
Cronobacter sakazakii is a xerotolerant neonatal pathogen epidemiologically linked to powdered infant food formula, often resulting in high mortality rates. Here, we used transcriptome sequencing (RNA-seq) to provide transcriptional insights into the survival of C. sakazakii in desiccated conditions. Our RNA-seq data show that about 22% of the total C. sakazakii genes were significantly...
Thu, 01/10/2019 - 00:00
Cronobacter species are a group of opportunistic food-borne pathogens that cause rare but severe infections in neonates. Tolerance to environmental stress in Cronobacter is known; however, factors involved in oxidative stress are undefined. In this study, Cronobacter sakazakii survival, cellular morphology, and biofilm formation in response to oxidative stress were evaluated between the wild type...
Fri, 12/28/2018 - 00:00
Abstract Essential oils (EOs) are liquid preparations produced from plant materials. Their use as inhibitors of the growth of spoilage and pathogenic microorganisms is a good alternative to the chemical additives in foods. The disc-diffusion method was used to screen the EOs from thyme, cinnamon, clove, peppermint, marjoram, cumin, rosemary, fennel, basil, lime, bergamot orange...
Wed, 12/26/2018 - 03:00
Journal of Food Protection, Volume 82, Issue 1, Page 65-77, January 2019. Cronobacter spp. are opportunistic human pathogens that cause serious diseases in neonates and immunocompromised people. Owing to their biofilm formation on various surfaces, both their detection and their removal from production plants constitute a major challenge. In this study, food samples were randomly collected in...
Tue, 12/18/2018 - 14:50
Journal of Food Safety, EarlyView. The aim of this study was to perform a molecular identification and design a polymerase chain reaction (PCR) method based on a specific gene (gyrase subunit B [gyrB]) for rapid detection of Cronobacter sakazakii. In this study, from February 2017 to January 2018, 100 powdered infant formula milks (PIF1‐6) and 100 baby food items (BF1‐8) (total number = 200...

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