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Enterohemorrhagic Escherichia coli

Thu, 05/16/2019 - 11:01
Microcin PDI (MccPDI), a class IIa microcin that is produced by Escherichia coli strains 25 and 284, is known to inhibit foodborne pathogenic enterohemorrhagic E. coli serotypes O157:H7 and O26. Here we demonstrate that MccPDI can inhibit Shigella strains and E. coli isolates that are multidrug resistant, the latter including strains known to cause urinary tract infections in people and companion...
Tue, 05/14/2019 - 15:38
Abstract The present study was undertaken to detect the occurrence of beta‐lactamase and biofilm producing Enterobacteriaceae in healthy ducks. A total 202 cloacal swabs were collected from ducks kept in organized (n=92) and backyard (n=110) farms in West Bengal (India). The ducks had no history of antibiotic intake. Among the 87 phenotypically beta‐lactamase producing E. coli, 19 (17·43%), six (...
Tue, 04/16/2019 - 01:10
Available online 13 April 2019 Author(s): Minerva Rivera-Reyes, Jonathan A. Campbell, Catherine N. CutterAbstractShiga toxin-producing Escherichia coli (STEC) are known to adapt and survive acidic conditions. Acid-adaptation also may result in cross-protection against other stressors present in foods, such as reduced water activity (aw), refrigeration conditions, or the presence of antimicrobials...
Wed, 04/10/2019 - 01:14
Available online 9 April 2019 Author(s): M. Pesciaroli, J.E. Chardon, E.H.M. Delfgou, A.F.A. Kuijpers, L.M. Wijnands, E.G. EversAbstractMicrobial survival of heating and cross-contamination are the two transmission routes during food preparation in the consumers' kitchen that are relevant for QMRA (Quantitative Microbial Risk Assessment). The aim of the present study was to extend the limited...
Tue, 04/09/2019 - 20:00
O-Antigen Gene Clusters of Plesiomonas shigelloides Serogroups and Its Application in Development of a Molecular Serotyping Scheme Daoyi Xi1,2,3,4†, Xiaochen Wang1,2,3,4†, Kexin Ning1,2,3,4, Qian Liu1,2,3,4, Fuyi Jing1,2,3,4, Xi Guo1,2,3,4 and Boyang Cao1,2,3,4* 1Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, TEDA College, Nankai University...
Mon, 04/08/2019 - 20:00
A Comparative Analysis of the Metabolomic Response of Electron Beam Inactivated E. coli O26:H11 and Salmonella Typhimurium ATCC 13311 Sohini S. Bhatia and Suresh D. Pillai* National Center for Electron Beam Research, International Atomic Energy Agency (IAEA), Collaborating Centre for Electron Beam Technology, Texas A&M University, College Station, TX, United States Ionizing radiation...
Fri, 03/29/2019 - 01:11
Publication date: Available online 26 March 2019Source: LWTAuthor(s): Edson A. Rios, Jesús Santos, Isidro García-Meniño, Saskia C. Flament-Simon, Jorge Blanco, María-Luisa García-López, Andrés Otero, Jose M. Rodríguez-CallejaAbstractThis study was carried out to determine the occurrence and characteristics of enteropathogenic Escherichia coli (EPEC) and Shiga toxin-producing E. coli (STEC)...
Mon, 03/25/2019 - 16:31
Publication date: May 2019 Environmental Research, Volume 172Author(s): Joseph Haymaker, Manan Sharma, Salina Parveen, Fawzy Hashem, Eric B. May, Eric T. Handy, Chanelle White, Cheryl East, Rhodel Bradshaw, Shirley A. Micallef, Mary Theresa Callahan, Sarah Allard, Brienna Anderson, Shani Craighead, Samantha Gartley, Adam Vanore, Kalmia E. Kniel, Sultana Solaiman, Anthony Bui, Rianna...
Sun, 03/24/2019 - 01:12
Available online 22 March 2019 Author(s): Alexander Gill, Catherine Carrillo, Micaela Hadley, Robyn Kenwell, Linda ChuiAbstractA 2016/2017 outbreak of Shiga toxin-producing Escherichia coli (STEC) O121 in Canada, was linked to wheat flour, milled at a single facility on three consecutive days in October 2016. Most Probable Number (MPN) estimates of the concentration of STEC O121 in the recalled...
Wed, 03/06/2019 - 11:00
We developed a multiplex PCR method based on multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) that was designed for the rapid typing of Escherichia coli and Shigella isolates. The method amplifies seven VNTRs and does not require a sequencing capillary or fluorescent dyes. The amplification products are simply loaded on a standard agarose gel for electrophoresis, and the...
Thu, 02/28/2019 - 01:10
Available online 26 February 2019 Author(s): Zeynal Topalcengiz, Michelle D. DanylukAbstractAgricultural water is considered as one of the main contamination source for produce prior to harvest. The purpose of study was to evaluate the fate of Shiga toxin-producing Escherichia coli (STEC), and generic E. coli in Central Florida agricultural surface water at different temperatures and the...
Fri, 02/22/2019 - 01:06
Fereidoun Forghani, Meghan den Bakker, Jye-Yin Liao, Alison S. Payton, Alexandra N. Futral, Francisco Diez-Gonzalez
Sat, 12/29/2018 - 01:13
Publication date: March 2019Source: LWT, Volume 102Author(s): Sailaja Chintagari, Raviraj Jadeja, Yen-Con HungAbstractThe efficacy of Infra-red radiation (IR) and Pulsed UV (PUV) for the inactivation of parent (Par) and Nalidixic acid adapted (Nal) strains of Escherichia coli O157:H7, top six shiga-toxin producing E. coli (E. coli O26, E. coli O45, E. coli O103, E. coli O111, E. coli O121, and E...
Sun, 12/23/2018 - 01:10
Publication date: March 2019Source: LWT, Volume 102Author(s): María Goretti Llamas-Arriba, Ángela Peirotén, Ana Isabel Puertas, Alicia Prieto, Paloma López, Miguel Á. Pardo, Eva Rodríguez, María Teresa DueñasAbstractBifidobacterium longum INIA P132 and Bifidobacterium infantis INIA P731, isolated from infant-faeces, were investigated in this work. Regarding the probiotic and technological...
Thu, 12/06/2018 - 01:10
Publication date: December 2018 , Volume 308, Issue 8Author(s): Inga Eichhorn, Torsten Semmler, Alexander Mellmann, Derek Pickard, Muna F. Anjum, Angelika Fruth, Helge Karch, Lothar H. WielerAbstractEnterohemorrhagic Escherichia coli (EHEC) are a cause of bloody diarrhea, hemorrhagic colitis (HC) and the potentially fatal hemolytic uremic syndrome (HUS). While O157:H7 is the dominant EHEC...
Sun, 09/23/2018 - 01:10
Available online 22 September 2018 Author(s): Prashant Singh, Yuejiao Liu, Joseph M. Bosilevac, Azlin MustaphaAbstractIn the United States, Shiga toxin-producing Escherichia coli (STEC) O157 and six non-O157 serogroups O26, O45, O103, O111, O121 and O145 are considered adulterants in non-intact beef. Further, Salmonella is responsible for one of the highest numbers of foodborne infections...
Mon, 07/02/2018 - 11:05
New Zealand has a relatively high incidence of human cases of Shiga toxin-producing Escherichia coli (STEC), with 8.9 STEC cases per 100,000 people reported in 2016. Previous research showed living near cattle and contact with cattle feces as significant risk factors for STEC infections in humans in New Zealand, but infection was not linked to food-associated factors. During the 2014 spring...
Wed, 06/20/2018 - 01:13
Publication date: Available online 19 June 2018 Author(s): Inga Eichhorn, Katrin Heidemanns, Rainer G. Ulrich, Herbert Schmidt, Torsten Semmler, Angelika Fruth, Astrid Bethe, David Goulding, Derek Pickard, Helge Karch, Lothar H. Wieler Bacteriophages play an important role in the evolution of bacterial pathogens. A phage-mediated transfer of stx-genes to atypical...
Mon, 06/18/2018 - 11:05
Wheat flour has been associated with outbreaks of enterohemorrhagic Escherichia coli (EHEC), but little is known on EHEC's survival during storage and thermal processing. The objective of this study was to determine long-term viability and thermal inactivation kinetics of EHEC serogroups O26, O103, O111, and O157. Wheat flour samples were inoculated with a cocktail of five strains of a single...
Thu, 06/14/2018 - 00:00
Toxins, Vol. 10, Pages 241: Toxins of Locus of Enterocyte Effacement-Negative Shiga Toxin-Producing Escherichia coli Toxins doi: 10.3390/toxins10060241 Authors: Maike Krause Holger Barth Herbert Schmidt Studies on Shiga toxin-producing Escherichia coli (STEC) typically examine and classify the virulence gene profiles based on genomic analyses. Among the screened strains, a subgroup of...
Tue, 05/29/2018 - 19:31
Publication date: Available online 29 May 2018 Author(s): Maren Mylius, Johannes Dreesman, Matthias Pulz, Gerhard Pallasch, Konrad Beyrer, Katja Claußen, Franz Allerberger, Angelika Fruth, Christina Lang, Rita Prager, Antje Flieger, Sabine Schlager, Daniela Kalhöfer, Elke Mertens Following a school ski-trip to Austria from 10-18/02/2017, nine of 25 participants of...
Mon, 05/07/2018 - 20:00
The formation of biofilms and subsequent encasement of bacterial cells in a complex matrix can enhance resistance to antimicrobials and sterilizing agents making these organisms difficult to eradicate and cont...
Sat, 04/14/2018 - 05:16
Publication date: August 2018 , Volume 142 Author(s): Marcelo Signorini, Magdalena Costa, David Teitelbaum, Viviana Restovich, Hebe Brasesco, Diego García, Valeria Superno, Sandra Petroli, Mariana Bruzzone, Victor Arduini, Mónica Vanzini, Adriana Sucari, Germán Suberbie, Turina Maricel, Ricardo Rodríguez, Gerardo A. Leotta In Argentina, Shiga toxin producing...
Wed, 12/27/2017 - 06:06
Abstract AimsThe purpose of this survey was to estimate the respective prevalence of the “gang of seven” and “non-gang of seven” serotypes of Shigatoxigenic and enteropathogenic Escherichia coli and to identify the O80:H2 serotype in 245 intestinal contents collected at two slaughterhouses in Belgium in 2014. Methods and ResultsAfter overnight enrichment growth the 69 intestinal contents testing...
Sun, 12/17/2017 - 21:15
Abstract The importance of wild birds as potential vectors of disease has received recent renewed empirical interest, especially regarding human health although information regarding the enteropathogenic bacteria in birds of prey continue to be scant. This study was performed with the aim to evaluate the occurrence of enteropathogenic bacteria (i.e. Campylobacter spp. Escherichia coli, Salmonella...
Fri, 12/15/2017 - 14:00
Publication date: Available online 9 December 2017 Author(s): Cecilia Cundon, Claudia Carolina Carbonari, Gisela Zolezzi, Marta Rivas, Adriana Bentancor Shiga toxin-producing Escherichia coli (STEC) is the etiological agent of gastrointestinal diseases as haemorrhagic colitis and haemolytic-uraemic syndrome (HUS). Shiga toxin (Stx) is the main virulence factor, there are two types...
Sun, 11/12/2017 - 18:52
Publication date: 2 January 2018 , Volume 264 Author(s): Wonhee Cha, Pina M. Fratamico, Leah E. Ruth, Andrew S. Bowman, Jacqueline M. Nolting, Shannon D. Manning, Julie A. Funk Shiga toxin-producing Escherichia coli (STEC) are important food-borne pathogens, which can cause serious illnesses, including hemorrhagic colitis and hemolytic uremic syndrome. To study the epidemiology...
Wed, 10/11/2017 - 01:30
Publication date: Available online 10 October 2017 Author(s): Wyatt Byrd, Fernando Ruiz-Perez, Prashanth Setty, Chengru Zhu, Edgar C. Boedeker We previously developed attenuated rabbit enteropathogenic E. coli (rEPEC) strains which are effective oral vaccines against their parent pathogens by deleting ler, a global regulator of virulence genes. To use these strains as orally...
Mon, 10/09/2017 - 15:39
Publication date: 16 November 2017 , Volume 261 Author(s): Christina Böhnlein, Jan Kabisch, Stefanie Müller-Herbst, Gregor Fiedler, Charles M.A.P. Franz, Rohtraud Pichner Fermented sausages have been identified as source of several outbreaks of Shiga toxin-producing Escherichia coli (STEC). Illnesses linked to non-O157 STEC serotypes appear to be on the rise worldwide, and...
Wed, 09/27/2017 - 04:10
Abstract The aim of this research was to optimize two multiplex polymerase chain reaction (PCR) assays that could simultaneously detect six non–O157 Shiga toxin–producing Escherichia coli (STEC) as well as the three virulence genes. We also investigated the potential of combining the FTA™ card-based DNA extraction with the multiplex PCR assays. Two multiplex PCR assays were optimized using six...
Tue, 09/26/2017 - 02:30
Abstract AimsTo determine the fate of E. coli on vegetables that were processed through commercial wash treatments and stored under simulated retail conditions at 4°C or wholesale at fluctuating ambient temperatures (0-25°C, dependent on season). Methods and ResultsBovine slurry that was naturally contaminated with Escherichia coli O145 was applied without dilution or diluted 1/10 using borehole...
Mon, 09/18/2017 - 00:26
Summary Shiga toxin-producing Escherichia coli (STEC) is a zoonotic pathogen of public health concern whose sources and transmission routes are difficult to trace. Using a combined source attribution and case–control analysis, we determined the relative contributions of four putative livestock sources (cattle, small ruminants, pigs, poultry) to human STEC infections and their associated dietary,...
Wed, 09/06/2017 - 01:10
Sabine Delannoy, Patricia Mariani-Kurkdjian, Hattie E. Webb, Stephane Bonacorsi, Patrick Fach
Thu, 08/31/2017 - 11:04
Shiga toxin-producing Escherichia coli (STEC) strains of the O91:H21 serotype have caused severe infections, including hemolytic-uremic syndrome. Strains of the O91 serogroup have been isolated from food, animals, and the environment worldwide but are not well characterized. We used a microarray and other molecular assays to examine 49 serogroup O91 strains (environmental, food, and clinical...
Thu, 08/31/2017 - 08:00
Publication date: February 2018 , Volume 84 Author(s): Alexandros Ch Stratakos, Filip Sima, Patrick Ward, Mark Linton, Carmel Kelly, Laurette Pinkerton, Lavinia Stef, Ioan Pet, Nicolae Corcionivoschi Shiga toxin producing Escherichia coli (STEC) are important pathogens implicated in foodborne outbreaks and severe human infections. E. coli O157:H7 is the most common strain...
Mon, 08/21/2017 - 20:00
Enterohemorrhagic Escherichia coli (EHEC) O145 are among the top non-O157 serogroups associated with severe human disease worldwide. Two serotypes, O145:H25 and O145:H28 have been isolated from human patients but...
Wed, 08/02/2017 - 12:15
Publication date: 16 October 2017 , Volume 259 Author(s): S. Balamurugan, Rafath Ahmed, Anli Gao, Phil Strange The study examined the relative fate of the top six non-O157 shiga-toxin producing Escherichia coli (STEC) and E. coli O157:H7 during the manufacture of dry fermented sausages (DFS). Three separate batches of sausages containing a five-strain cocktail for each serogroup and...
Fri, 07/28/2017 - 23:40
Summary Feeding high levels (≥40% dry matter) of distillers grains may increase the risk for cattle to carry enterohemorrhagic Escherichia coli (EHEC) O157. The mechanism for the increased risk is not known nor whether non-O157 EHEC are similarly affected. Our objective was to test whether the fibre content or other components of modified distillers grains plus solubles (MDGS) affects the...
Fri, 07/21/2017 - 11:38
Publication date: September 2017 , Volume 140 Author(s): Waldemar Rastawicki, Anna Chróst, Kornelia Gielarowiec Latex agglutination tests (LAT) were developed and evaluated for the rapid detection of LPS antibodies against E. coli serogroup O157, O26, O104, O111 and O145. The latex tests have been proved to be sensitive, fast, easy-to-perform and cost-efficient tools for the screening...
Sat, 07/01/2017 - 10:45
Publication date: 18 September 2017 , Volume 257 Author(s): Gaia Nobili, Ilenia Franconieri, Gianfranco La Bella, Maria Grazia Basanisi, Giovanna La Salandra Verocytotoxin (VT)-producing Escherichia coli (VTEC) are a significant foodborne public health hazard, where most human infections are associated with six serogroups (O157, O26, O103, O145, O111 and O104). VTEC was the fourth...
Thu, 06/29/2017 - 00:09
Publication date: Available online 29 June 2017 Author(s): L. Martorelli, A. Albanese, D. Vilte, R. Cantet, A. Bentancor, G. Zolezzi, I. Chinen, C. Ibarra, M. Rivas, E.C. Mercado, A. Cataldi Problem addressed Shiga toxin-producing Escherichia coli (STEC) are a group of bacteria responsible for food-associated diseases. Clinical features include a wide range of symptoms...
Thu, 06/01/2017 - 14:57
Publication date: Available online 1 June 2017 Author(s): Carla Andrea Alonso, Azucena Mora, Dafne Díaz, Miguel Blanco, David González-Barrio, Francisco Ruiz-Fons, Carmen Simón, Jorge Blanco, Carmen Torres Shiga toxin-producing E. coli (STEC) and enteropathogenic E. coli (EPEC) strains are food-borne pathogens associated with acute diarrhea. Haemolytic-uremic syndrome (HUS...
Fri, 04/21/2017 - 07:18
Publication date: Available online 21 April 2017 Author(s): T. Douëllou, S. Delannoy, S. Ganet, P. Fach, E. Loukiadis, M.-C. Montel, D. Sergentet-Thevenot Pathogenic Shiga toxin-producing E. coli (STEC) are recognized worldwide as environment and foodborne pathogens which can be transmitted by ingestion of ready-to-eat food such as raw milk-derived products. STEC show a...
Thu, 04/20/2017 - 01:12
Ken-ichi Lee, Tomoko Morita-Ishihara, Sunao Iyoda, Yoshitoshi Ogura, Tetsuya Hayashi, Tsuyoshi Sekizuka, Makoto Kuroda, Makoto Ohnishi, EHEC Working Group in Japan , Hiroko Takenuma, Junji Seto, Yu Suzuki, Kyoko Mashiko, Shigenori Matsui, Shinichiro Hirai, Eiji Yokoyama, Noriko Konishi, Hiromi Obata, Akemi Kai, Atsuko Ogawa, Yuko Matsumoto, Ayako Kikuchi, Emiko Kitagawa, Hitomi Kasahara, Maki...

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