Enterohemorrhagic Escherichia coli

Fri, 11/08/2019 - 03:34
Author(s): Minto Michael, Jennifer Acuff, Keyla Lopez, Daniel Vega, Randall Phebus, Harshavardhan Thippareddi, Lakshmikantha H. ChannaiahAbstractThis study was conducted to validate a simulated commercial baking process for plain muffins against E. coli O121 (isolated from the recent illness outbreak associated with flour), and compare the thermal inactivation parameters (D- and z-values) of...
Thu, 11/07/2019 - 03:29
This study investigated the occurrence of shiga toxin‐producing Escherichia coli (STEC), thermotolerant Campylobacter spp. and Salmonella spp. in Swedish dairy milk. A total of 302 inline milk filters were analyzed. Salmonella was not isolated from any filters. Polymerase chain reaction screening detected thermotolerant Campylobacter in 30.5% of the milk filters analyzed and it was isolated from...
Wed, 10/30/2019 - 03:34
Author(s): E. Frank, R. Bonke, N. Drees, M. Heurich, E. Märtlbauer, M. GareisAbstractWorldwide infections by Shiga toxin-producing Escherichia coli (STEC) in humans have been reported after consumption of mainly beef, but also deer meat. Not only the consumption of contaminated deer meat represents a risk, but also the transmission of STEC between deer and domestic animals should be considered....
Sat, 10/26/2019 - 03:36
Shiga toxin‐producing Escherichia coli (STEC) is a zoonotic pathotype associated with human gastrointestinal disease that may progress to severe complications. Ruminants, especially cattle, are the main reservoirs of STEC from which they can contaminate the environment and foods of animal or vegetable origin. Besides Shiga toxin, other virulence factors are involved in STEC virulence. O157:H7...
Thu, 10/24/2019 - 03:43
Author(s): Anna Szczerba-Turek, Jan Siemionek, Piotr Socha, Agata Bancerz-Kisiel, Aleksandra Platt-Samoraj, Karolina Lipczynska-Ilczuk, Wojciech SzwedaABSTRACTShiga toxin-producing Escherichia (E.) coli (STEC) pathogens are responsible for the outbreaks of serious diseases in humans, including haemolytic uraemic syndrome (HUS), bloody diarrhoea (BD) and diarrhoea (D), and they pose a significant...
Fri, 10/11/2019 - 03:41
We report high-quality closed reference genomes for 1 bovine strain and 10 human Shiga toxin (Stx)-producing Escherichia coli (STEC) strains from serogroups O26, O45, O91, O103, O104, O111, O113, O121, O145, and O157. We also report draft assemblies, with standardized metadata, for 360 STEC strains isolated from watersheds, animals, farms, food, and human infections.
Fri, 10/04/2019 - 03:42
We present the complete genome sequence of an enterohemorrhagic Escherichia coli O111:H8 strain. This strain was isolated from a hemolytic-uremic syndrome patient and was responsible for a large outbreak associated with the consumption of raw beef in 2011.
Wed, 10/02/2019 - 03:41
Certain foodborne Shiga toxin-producing Escherichia coli (STEC) strains carry genes encoding the subtilase cytotoxin (SubAB). Although the mode of action of SubAB is under intensive investigation, information about the regulation of subAB gene expression is currently not available. In this study, we investigated the regulation of the chromosomal subAB1 gene in laboratory E. coli strain DH5...
Mon, 09/30/2019 - 17:10
Shiga toxin-producing Escherichia coli (STEC) O145 is one of the most prevalent non-O157 serogroups associated with foodborne outbreaks. Lytic phages are a potential alternative to antibiotics in combatting bacterial pathogens. In this study, we characterized a Siphoviridae phage lytic against STEC O145 strains as a novel antimicrobial agent. Escherichia phage vB_EcoS-Ro145clw (Ro145clw) was...
Thu, 09/12/2019 - 03:39
Author(s): Ronald Lulijwa, Andrea C. Alfaro, Fabrice Merien, Mark Burdass, Leonie Venter, Tim YoungAbstractWe investigated cellular functional and targeted immune cytokine responses of farmed Chinook salmon (Oncorhynchus tshawytscha) peripheral blood mononuclear cells (PBMCs) in vitro to LPS from Escherichia coli (E. coli) serotypes O111: B4 and O55: B5, and a phorbol ester phorbol 12-myristate...
Mon, 08/26/2019 - 01:05
Available online 25 August 2019 Author(s): Guerrino Macori, Siobhán C. McCarthy, Catherine M. Burgess, Séamus Fanning, Geraldine DuffyAbstractA quantitative PCR method is described for the detection and quantification of E. coli O157 and O26 in sheep recto-anal junction swabs. The method incorporated a short enrichment step (5 h) and the use of a developed standard calibration curve relating the...
Fri, 08/09/2019 - 15:58
Abstract Aims First, two inactivation models were compared for different phenotypic profiles of Escherichia coli O26 using ultraviolet‐C light (UV‐C) and thermal treatment (T), by means of Central Composite Rotatable Design of Experiment (CCRD). Second, we aimed to evaluate the subsequent survival and persistence of cells in simulated gastric fluid (SGF). Methods and Results Two strains of E....
Tue, 07/30/2019 - 17:00
Narjol González-Escalona, Marc A. Allard, Eric W. Brown, Shashi Sharma, Maria Hoffmann Whole genome sequencing can provide essential public health information. However, it is now known that widely used short-read methods have the potential to miss some randomly-distributed segments of genomes. This can prevent phages, plasmids, and virulence factors from being detected or properly identified....
Mon, 07/22/2019 - 05:03
Abstract Aims First, two inactivation models were compared for different phenotypic profiles of Escherichia coli O26 using UV‐C light (UV‐C) and thermal treatment (T), by means of Central Composite Rotatable Design of Experiment (CCRD). Second, we aimed to evaluate subsequent survival and persistence of cells in simulated gastric fluid (SGF). Methods and Results Two strains of E. coli O26, a...
Fri, 07/19/2019 - 00:00
Toxins, Vol. 11, Pages 424: Virulence Characteristics and Antimicrobial Resistance Profiles of Shiga Toxin-Producing Escherichia coli Isolates from Humans in South Africa: 2006–2013 Toxins doi: 10.3390/toxins11070424 Authors: Karama Cenci-Goga Malahlela Smith Keddy El-Ashram Kabiru Kalake Shiga toxin-producing Escherichia coli (STEC) isolates (N = 38) that were...
Thu, 07/18/2019 - 01:05
Available online 16 July 2019 Author(s): Magdalena Costa, Adriana Sucari, Sergio Epszteyn, Juan Oteiza, Jimena Gentiluomo, Celia Melamed, Yamila Figueroa, Santiago Mingorance, Agustina Grisaro, Silvia Spioussas, Mariana Buffoni Almeida, Mailen Caruso, Andrés Pontoni, Marcelo Signorini, Gerardo LeottaAbstractShiga toxin-producing Escherichia coli (STEC) are important pathogens transmitted by food...
Tue, 07/16/2019 - 03:00
Journal of Food Protection, Volume 82, Issue 8, Page 1308-1313, August 2019.
Mon, 07/15/2019 - 01:05
Available online 14 July 2019 Author(s): Carlota Cedillo Martin, Cecilie Smith Svanevik, Bjørn Tore Lunestad, Camilla Sekse, Gro S. JohannessenAbstractOnly a few studies concerning Shiga toxin-producing E. coli (STEC) detection in bivalves and their harvesting areas have been reported, and to the best of our knowledge there are no outbreaks associated with STEC from bivalves described. The aim of...
Fri, 05/31/2019 - 01:12
Available online 29 May 2019 Author(s): Saida Essendoubi, Natisha Stashko, Iyla So, Gary Gensler, Deana Rolheiser, Chunu MainaliAbstractFrom January to December 2016, Alberta Agriculture and Forestry (AAF) conducted a provincial survey of selected pathogens and indicator organisms on beef carcasses processed at Provincially Licensed Abattoirs (PLAs) in Alberta. The survey was conducted in seven...
Thu, 05/16/2019 - 11:01
Microcin PDI (MccPDI), a class IIa microcin that is produced by Escherichia coli strains 25 and 284, is known to inhibit foodborne pathogenic enterohemorrhagic E. coli serotypes O157:H7 and O26. Here we demonstrate that MccPDI can inhibit Shigella strains and E. coli isolates that are multidrug resistant, the latter including strains known to cause urinary tract infections in people and companion...
Tue, 05/14/2019 - 15:38
Abstract The present study was undertaken to detect the occurrence of beta‐lactamase and biofilm producing Enterobacteriaceae in healthy ducks. A total 202 cloacal swabs were collected from ducks kept in organized (n=92) and backyard (n=110) farms in West Bengal (India). The ducks had no history of antibiotic intake. Among the 87 phenotypically beta‐lactamase producing E. coli, 19 (17·43%), six (...
Tue, 04/16/2019 - 01:10
Available online 13 April 2019 Author(s): Minerva Rivera-Reyes, Jonathan A. Campbell, Catherine N. CutterAbstractShiga toxin-producing Escherichia coli (STEC) are known to adapt and survive acidic conditions. Acid-adaptation also may result in cross-protection against other stressors present in foods, such as reduced water activity (aw), refrigeration conditions, or the presence of antimicrobials...
Wed, 04/10/2019 - 01:14
Available online 9 April 2019 Author(s): M. Pesciaroli, J.E. Chardon, E.H.M. Delfgou, A.F.A. Kuijpers, L.M. Wijnands, E.G. EversAbstractMicrobial survival of heating and cross-contamination are the two transmission routes during food preparation in the consumers' kitchen that are relevant for QMRA (Quantitative Microbial Risk Assessment). The aim of the present study was to extend the limited...
Tue, 04/09/2019 - 20:00
O-Antigen Gene Clusters of Plesiomonas shigelloides Serogroups and Its Application in Development of a Molecular Serotyping Scheme Daoyi Xi1,2,3,4†, Xiaochen Wang1,2,3,4†, Kexin Ning1,2,3,4, Qian Liu1,2,3,4, Fuyi Jing1,2,3,4, Xi Guo1,2,3,4 and Boyang Cao1,2,3,4* 1Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, TEDA College, Nankai University...
Mon, 04/08/2019 - 20:00
A Comparative Analysis of the Metabolomic Response of Electron Beam Inactivated E. coli O26:H11 and Salmonella Typhimurium ATCC 13311 Sohini S. Bhatia and Suresh D. Pillai* National Center for Electron Beam Research, International Atomic Energy Agency (IAEA), Collaborating Centre for Electron Beam Technology, Texas A&M University, College Station, TX, United States Ionizing radiation...
Fri, 03/29/2019 - 01:11
LWT
Publication date: Available online 26 March 2019Source: LWTAuthor(s): Edson A. Rios, Jesús Santos, Isidro García-Meniño, Saskia C. Flament-Simon, Jorge Blanco, María-Luisa García-López, Andrés Otero, Jose M. Rodríguez-CallejaAbstractThis study was carried out to determine the occurrence and characteristics of enteropathogenic Escherichia coli (EPEC) and Shiga toxin-producing E. coli (STEC)...
Mon, 03/25/2019 - 16:31
Publication date: May 2019 Environmental Research, Volume 172Author(s): Joseph Haymaker, Manan Sharma, Salina Parveen, Fawzy Hashem, Eric B. May, Eric T. Handy, Chanelle White, Cheryl East, Rhodel Bradshaw, Shirley A. Micallef, Mary Theresa Callahan, Sarah Allard, Brienna Anderson, Shani Craighead, Samantha Gartley, Adam Vanore, Kalmia E. Kniel, Sultana Solaiman, Anthony Bui, Rianna...
Sun, 03/24/2019 - 01:12
Available online 22 March 2019 Author(s): Alexander Gill, Catherine Carrillo, Micaela Hadley, Robyn Kenwell, Linda ChuiAbstractA 2016/2017 outbreak of Shiga toxin-producing Escherichia coli (STEC) O121 in Canada, was linked to wheat flour, milled at a single facility on three consecutive days in October 2016. Most Probable Number (MPN) estimates of the concentration of STEC O121 in the recalled...
Wed, 03/06/2019 - 11:00
We developed a multiplex PCR method based on multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) that was designed for the rapid typing of Escherichia coli and Shigella isolates. The method amplifies seven VNTRs and does not require a sequencing capillary or fluorescent dyes. The amplification products are simply loaded on a standard agarose gel for electrophoresis, and the...
Thu, 02/28/2019 - 01:10
Available online 26 February 2019 Author(s): Zeynal Topalcengiz, Michelle D. DanylukAbstractAgricultural water is considered as one of the main contamination source for produce prior to harvest. The purpose of study was to evaluate the fate of Shiga toxin-producing Escherichia coli (STEC), and generic E. coli in Central Florida agricultural surface water at different temperatures and the...
Fri, 02/22/2019 - 01:06
Fereidoun Forghani, Meghan den Bakker, Jye-Yin Liao, Alison S. Payton, Alexandra N. Futral, Francisco Diez-Gonzalez
Sat, 12/29/2018 - 01:13
LWT
Publication date: March 2019Source: LWT, Volume 102Author(s): Sailaja Chintagari, Raviraj Jadeja, Yen-Con HungAbstractThe efficacy of Infra-red radiation (IR) and Pulsed UV (PUV) for the inactivation of parent (Par) and Nalidixic acid adapted (Nal) strains of Escherichia coli O157:H7, top six shiga-toxin producing E. coli (E. coli O26, E. coli O45, E. coli O103, E. coli O111, E. coli O121, and E...
Sun, 12/23/2018 - 01:10
LWT
Publication date: March 2019Source: LWT, Volume 102Author(s): María Goretti Llamas-Arriba, Ángela Peirotén, Ana Isabel Puertas, Alicia Prieto, Paloma López, Miguel Á. Pardo, Eva Rodríguez, María Teresa DueñasAbstractBifidobacterium longum INIA P132 and Bifidobacterium infantis INIA P731, isolated from infant-faeces, were investigated in this work. Regarding the probiotic and technological...
Thu, 12/06/2018 - 01:10
Publication date: December 2018 , Volume 308, Issue 8Author(s): Inga Eichhorn, Torsten Semmler, Alexander Mellmann, Derek Pickard, Muna F. Anjum, Angelika Fruth, Helge Karch, Lothar H. WielerAbstractEnterohemorrhagic Escherichia coli (EHEC) are a cause of bloody diarrhea, hemorrhagic colitis (HC) and the potentially fatal hemolytic uremic syndrome (HUS). While O157:H7 is the dominant EHEC...
Sun, 09/23/2018 - 01:10
Available online 22 September 2018 Author(s): Prashant Singh, Yuejiao Liu, Joseph M. Bosilevac, Azlin MustaphaAbstractIn the United States, Shiga toxin-producing Escherichia coli (STEC) O157 and six non-O157 serogroups O26, O45, O103, O111, O121 and O145 are considered adulterants in non-intact beef. Further, Salmonella is responsible for one of the highest numbers of foodborne infections...
Mon, 07/02/2018 - 11:05
New Zealand has a relatively high incidence of human cases of Shiga toxin-producing Escherichia coli (STEC), with 8.9 STEC cases per 100,000 people reported in 2016. Previous research showed living near cattle and contact with cattle feces as significant risk factors for STEC infections in humans in New Zealand, but infection was not linked to food-associated factors. During the 2014 spring...
Wed, 06/20/2018 - 01:13
Publication date: Available online 19 June 2018 Author(s): Inga Eichhorn, Katrin Heidemanns, Rainer G. Ulrich, Herbert Schmidt, Torsten Semmler, Angelika Fruth, Astrid Bethe, David Goulding, Derek Pickard, Helge Karch, Lothar H. Wieler Bacteriophages play an important role in the evolution of bacterial pathogens. A phage-mediated transfer of stx-genes to atypical...
Mon, 06/18/2018 - 11:05
Wheat flour has been associated with outbreaks of enterohemorrhagic Escherichia coli (EHEC), but little is known on EHEC's survival during storage and thermal processing. The objective of this study was to determine long-term viability and thermal inactivation kinetics of EHEC serogroups O26, O103, O111, and O157. Wheat flour samples were inoculated with a cocktail of five strains of a single...
Thu, 06/14/2018 - 00:00
Toxins, Vol. 10, Pages 241: Toxins of Locus of Enterocyte Effacement-Negative Shiga Toxin-Producing Escherichia coli Toxins doi: 10.3390/toxins10060241 Authors: Maike Krause Holger Barth Herbert Schmidt Studies on Shiga toxin-producing Escherichia coli (STEC) typically examine and classify the virulence gene profiles based on genomic analyses. Among the screened strains, a subgroup of...
Tue, 05/29/2018 - 19:31
Publication date: Available online 29 May 2018 Author(s): Maren Mylius, Johannes Dreesman, Matthias Pulz, Gerhard Pallasch, Konrad Beyrer, Katja Claußen, Franz Allerberger, Angelika Fruth, Christina Lang, Rita Prager, Antje Flieger, Sabine Schlager, Daniela Kalhöfer, Elke Mertens Following a school ski-trip to Austria from 10-18/02/2017, nine of 25 participants of...
Mon, 05/07/2018 - 20:00
The formation of biofilms and subsequent encasement of bacterial cells in a complex matrix can enhance resistance to antimicrobials and sterilizing agents making these organisms difficult to eradicate and cont...
Sat, 04/14/2018 - 05:16
Publication date: August 2018 , Volume 142 Author(s): Marcelo Signorini, Magdalena Costa, David Teitelbaum, Viviana Restovich, Hebe Brasesco, Diego García, Valeria Superno, Sandra Petroli, Mariana Bruzzone, Victor Arduini, Mónica Vanzini, Adriana Sucari, Germán Suberbie, Turina Maricel, Ricardo Rodríguez, Gerardo A. Leotta In Argentina, Shiga toxin producing...
Wed, 12/27/2017 - 06:06
Abstract AimsThe purpose of this survey was to estimate the respective prevalence of the “gang of seven” and “non-gang of seven” serotypes of Shigatoxigenic and enteropathogenic Escherichia coli and to identify the O80:H2 serotype in 245 intestinal contents collected at two slaughterhouses in Belgium in 2014. Methods and ResultsAfter overnight enrichment growth the 69 intestinal contents testing...
Sun, 12/17/2017 - 21:15
Abstract The importance of wild birds as potential vectors of disease has received recent renewed empirical interest, especially regarding human health although information regarding the enteropathogenic bacteria in birds of prey continue to be scant. This study was performed with the aim to evaluate the occurrence of enteropathogenic bacteria (i.e. Campylobacter spp. Escherichia coli, Salmonella...
Fri, 12/15/2017 - 14:00
Publication date: Available online 9 December 2017 Author(s): Cecilia Cundon, Claudia Carolina Carbonari, Gisela Zolezzi, Marta Rivas, Adriana Bentancor Shiga toxin-producing Escherichia coli (STEC) is the etiological agent of gastrointestinal diseases as haemorrhagic colitis and haemolytic-uraemic syndrome (HUS). Shiga toxin (Stx) is the main virulence factor, there are two types...

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