Enterohemorrhagic Escherichia coli

Wed, 07/01/2020 - 06:10
LWT
Author(s): Prashant Singh, Gabriel Cubillos, Gabrielle Kirshteyn, Joseph M. Bosilevac Strains of Shiga toxin-producing Escherichia coli (STEC) are major foodborne pathogens. These strains are frequently involved in foodborne outbreaks. The aim of this study was to develop high-resolution melting (HRM) real-time PCR assays for the specific detection of O26 and O111. Then apply HRM analysis for...
Mon, 06/22/2020 - 06:10
Author(s): Changbao Wang, Hua Hang, Shoubiao Zhou, Yan D. Niu, Hechao Du, Kim Stanford, Tim A. McAllister Shiga toxigenic Escherichia coli (STEC) can form biofilms and frequently cause serious foodborne illnesses. A strain of STEC O145:H25 (EC19990166) known to be a strong biofilm former was used to evaluate the efficacy of bacteriophage AZO145A against biofilms formed on stainless steel (SS)...
Thu, 06/18/2020 - 10:02
Decontamination practices, which often involve thermal treatments, are routinely performed in beef packing plants and have generally improved the safety of meat in North America. We investigated whether Escherichia coli in the beef production chain is becoming more heat resistant due to those treatments. Cattle isolates (n = 750) included seven serogroups (O157, O103, O111, O121, O145, O26, and...
Thu, 06/11/2020 - 00:00
Calf diarrhea is one of the considerable infectious diseases in calves, which results in tremendous economic losses globally. To determine the prevalence of Shiga-toxigenic E. coli (STEC) and Enterotoxigenic E. coli (ETEC) incriminated in calf diarrhea, with special reference to Shiga- toxins genes (stx1 and stx2) and enterotoxins genes (lt and sta) that govern their pathogenesis, as well as the...
Sun, 06/07/2020 - 22:13
Aims The goal was to explore the effects of subinhibitory concentration (SIC) (0.5 MIC=20 µg L‐1) of ciprofloxacin on the transcriptome of enterohemorrhagic Escherichia coli O26:H11 (EHEC) isolate by 60 minutes of exposure. Materials and Results We used a combination of comparative genomic and transcriptomic (RNAseq) analyses. The whole genome of the E. coli O26:H11 #30934 strain of bovine origin...
Sat, 06/06/2020 - 04:03
There is increasing evidence that diversity changes in bacterial communities of beef cattle correlates to the presence of Shiga toxin-producing Escherichia coli (STEC). However, studies that found an association between STEC and bacterial diversity have been focused on pre-slaughter stages in the beef supply chain. This study was designed to test a hypothesis that there is no differences in...
Sat, 05/16/2020 - 03:36
Bacteriophages, natural killers of bacteria, and plant secondary metabolites, such as condensed tannins, are potential agents for the control of foodborne pathogens. The first objective of this study evaluated the efficacy of a bacteriophage SA21RB in reducing pre-formed biofilms on stainless-steel produced by two Shiga toxin-producing Escherichia coli (STEC) strains, one from South Africa and...
Sun, 05/03/2020 - 03:36
In this work, we present a magnetic bead–based immunosensorss for the determination of Escherichia coli O26 in milk. The assay uses commercial Dynabeads® for the capture of the target bacteria from 6-h enrichment broth and horseradish peroxidase–labelled polyclonal antibodies and detection of the peroxidase activity by chronoamperometry. Bovine seroalbumin was used as blocking reagent for...
Wed, 04/22/2020 - 03:44
This research study was based on ultraviolet (UVC) light and a combination of UVC-ozone treatments that have recently received attention from the beef processing industry as antimicrobial interventions that leave no chemical residues on products. The objectives were to evaluate effectiveness of UVC and UVC in combination with gaseous ozone treatments for inactivation of pathogenic bacteria on...
Sun, 04/19/2020 - 03:46
Shiga toxin-producing Escherichia coli (STEC) strains are important zoonotic foodborne pathogens, causing diarrhea, hemorrhagic colitis, and life-threatening hemolytic uremic syndrome (HUS) in humans. However, antibiotic treatment of STEC infection is associated with an increased risk of HUS. Therefore, there is an urgent need for early and effective therapeutic strategies. Here, we isolated...
Fri, 04/17/2020 - 03:35
Domestic and wild animal intrusions are identified as a food safety risk during fresh produce production. The purpose of this study was to evaluate the survival of Shiga toxin-producing Escherichia coli (STEC) in cattle, feral pig, waterfowl, deer, and raccoon feces from sources in California, Delaware, Florida, and Ohio. Fecal samples were inoculated with a cocktail of rifampicin resistant STEC...
Sat, 04/11/2020 - 03:29
Typical hemolytic uremic syndrome (STEC‐HUS), caused by Shiga toxin (Stx)‐producing Escherichia coli (STEC), is a serious, life‐threating disease that mainly affects children. Bacteriological and genetic tests are commonly used in the routine laboratory diagnosis of STEC‐HUS; however, serological methods have emerged as useful and reliable diagnostic tools, especially when bacterial isolation...
Sat, 04/04/2020 - 03:43
It is well established that intravenous administration of lipopolysaccharides (LPS)—cell wall components from gram-negative bacteria—induce acute inflammatory responses in dairy calves, but the effect of oral administration of LPS to dairy calves is currently unknown. To evaluate the effects of oral administration of LPS derived from Escherichia coli (serotype O111:B4) on innate immune responses...
Thu, 04/02/2020 - 03:32
Author(s): Marie Frétin, Christophe Chassard, Céline Delbès, René Lavigne, Etienne Rifa, Sébastien Theil, Benoit Fernandez, Patrice Laforce, Cécile Callon Safety in raw milk cheeses being a major public health issue, the aim of this study was to validate a new bio preservation strategy by evaluating the efficacy of an inhibitory bacterial consortium (Hafnia alvei, Lactobacillus plantarum and...
Tue, 03/31/2020 - 03:26
Shiga toxin-producing Escherichia coli (STEC) are important foodborne pathogens and non-O157 serotypes have been gradually increasing in frequency. The non-O157 STEC population is diverse and is often characterized using serotyping and/or multilocus sequence typing (MLST). Although spacers within clustered regularly interspaced repeat (CRISPR) regions were shown to comprise horizontally acquired...
Fri, 03/27/2020 - 04:04
by Mariana Cap, Celeste Cingolani, Carla Lires, Marina Mozgovoj, Trinidad Soteras, Adriana Sucari, Jimena Gentiluomo, Adriana Descalzo, Gabriela Grigioni, Marcelo Signorini, Celina Horak, Sergio Vaudagna, Gerardo Leotta The aim of this study was to assess the efficacy of lactic acid (LA), caprylic acid (CA), high- (HDI) and low- (LDI) dose gamma irradiation and LDI combined with LA or CA on the...
Wed, 03/18/2020 - 03:33
This study examined the biofilm-forming ability of six non-O157 Shiga-toxin-producing Escherichia coli (STEC) strains: O116:H21, wzx-Onovel5:H19, O129:H21, O129:H23, O26:H11, and O154:H10 on stainless steel coupons after 24, 48, and 72 h of incubation at 22 °C and after 168 h at 10 °C. The results of crystal violet staining revealed that strains O129:H23 and O154:H10 were able to form biofilms on...
Fri, 03/06/2020 - 04:07
Shiga toxin-producing Escherichia coli (STEC) is a foodborne disease with worldwide outbreaks. STEC serotypes O157, O26, O45, O103, O111, O121, and O145 cause the most outbreaks. There is little published information regarding the other serotypes. We report the draft genome sequences for 11 uncommon STEC serotypes from Nebraska.
Wed, 03/04/2020 - 01:18
Recent outbreaks traced to contaminated flour have created a need in the milling industry for a process that reduces pathogens in wheat while maintaining its functional properties. Vacuum steam treatment is a promising technology for treatment of low moisture foods. Traditional thermal treatment methods can compromise wheat functionality due to high temperatures, thus maintaining the functional...
Tue, 02/25/2020 - 03:41
Escherichia coli serogroups O157, O26, O45, O103, O111, O121, and O145, when carrying major virulence genes, the Shiga toxin genes stx1 and stx2 and the intimin gene eae, are important foodborne pathogens. They are referred to as the "top 7" Shiga toxin-producing E. coli (STEC) serogroups and were declared by the USDA as adulterants to human health. Since top 7 serogroup-positive cattle feces and...
Fri, 02/21/2020 - 03:52
Extended-spectrum β-lactamase (ESBL)-producing bacteria constitute an emerging global health issue with food products being vehicles of transmission and the aquatic environments serving as potential reservoirs. This study aimed to characterize ESBL-producing Escherichia coli in Nile perch and water from Lake Victoria in Tanzania. A total of 180 samples of Nile perch and 60 water samples were...
Wed, 02/12/2020 - 10:50
Author(s): Marina C. Lamparter, Annica Seemann, Carolin Hobe, Elisabeth SchuhAbstractShiga toxin-producing Escherichia coli (STEC) in sprouts have caused large scale outbreaks in the past involving severe illness. The combination of this very diverse pathogen and a food matrix with high numbers of background microbiota poses a particular challenge for detection and isolation. An acid treatment of...
Wed, 01/22/2020 - 03:35
Current California agricultural practices strive to comanage food safety and habitat conservation on farmland. However, the ecology of foodborne pathogens in wild bird populations, especially those avian species residing in proximity to fresh produce production fields, is not fully understood. In this repeated cross-sectional study, avifauna within agricultural lands in California were sampled...
Tue, 01/21/2020 - 03:23
aEPEC are associated with persistent diarrhea, and diarrheal outbreaks in both humans and animals worldwide. They are differentiated from typical EPEC by the lack of bundle-forming pili, and from EHEC by the lack of phage-mediated stx toxins. However, phylogenetic analyses often associate aEPEC with EHEC, promoting the hypothesis that aEPEC are the progenitors of EHEC, which is supported by aEPEC...
Wed, 01/15/2020 - 03:23
Recent outbreaks traced to contaminated flour have created a need in the milling industry for a process that reduces pathogens in wheat while maintaining its functional properties. Vacuum steam treatment is a promising technology for treatment of low moisture foods. Traditional thermal treatment methods can compromise wheat functionality due to high temperatures, thus maintaining the functional...
Wed, 01/15/2020 - 03:23
One intrinsic characteristic of low-moisture foods that is frequently overlooked is pH. Although pH affects the survival of microorganisms in high-moisture foods, its influence in low-moisture foods with less available moisture has not been examined. Escherichia coli O157:H7, E. coli O121, Salmonella enterica Anatum, and S. enterica Agona were grown on solid media with and without added glucose,...
Tue, 12/31/2019 - 04:00
Author(s): Ayanna Glaize, Eduardo Gutierrez-Rodriguez, Irene Hanning, Sandra Díaz-Sánchez, Chris Gunter, Arnoud H.M. van Vliet, Wes Watson, Siddhartha ThakurAbstractThe interaction of typical host adapted enteric bacterial pathogens with fresh produce grown in fields is complex. These interactions can be more pronounced in co-managed or sustainable farms where animal operations are, by design,...
Tue, 12/24/2019 - 03:28
Shiga toxin‐producing Escherichia coli (STEC) can cause diarrhoea and severe diseases in humans, such as haemolytic uraemic syndrome. STEC virulence is considered to correlate with the amount of Shiga toxins (Stx) produced, especially Stx2, whose subtype Stx2a is most frequently associated with high virulence. Stx are encoded in prophages, which play an important role in STEC pathogenesis. The...
Thu, 12/12/2019 - 03:38
Author(s): Lin Chen, Xue Zhao, Ji'en Wu, Qin Liu, Xinyi Pang, Hongshun YangAbstractThe metabolic diversity of Escherichia coli strains (non-pathogenic E. coli ATCC 25922, and pathogenic E. coli O157:H7, O26:H11, O45:H2, O103:H11, O111, O121:H19, and O145) was tested using nuclear magnetic resonance. Based on two representative two-dimensional 1H-13C spectra, 38 metabolites were identified in E....
Wed, 11/20/2019 - 04:13
by Hiroaki Baba, Hajime Kanamori, Hayami Kudo, Yasutoshi Kuroki, Seiya Higashi, Kentaro Oka, Motomichi Takahashi, Makiko Yoshida, Kengo Oshima, Tetsuji Aoyagi, Koichi Tokuda, Mitsuo Kaku Shiga toxin-producing Escherichia coli (STEC) can cause severe gastrointestinal disease and colonization among food handlers. In Japan, STEC infection is a notifiable disease, and food handlers are required to...
Wed, 11/20/2019 - 04:05
Author(s): Alexander Gill, Tanis McMahon, Forest Dussault, Nicholas PetronellaAbstractWheat flour has recently been recognised as an exposure vehicle for the foodborne pathogen Shiga toxin-producing Escherichia coli (STEC). Wheat flour milled on two sequential production days in October 2016, and implicated in a Canada wide outbreak of STEC O121:H19, was analysed for the presence of STEC in...
Fri, 11/08/2019 - 03:34
Author(s): Minto Michael, Jennifer Acuff, Keyla Lopez, Daniel Vega, Randall Phebus, Harshavardhan Thippareddi, Lakshmikantha H. ChannaiahAbstractThis study was conducted to validate a simulated commercial baking process for plain muffins against E. coli O121 (isolated from the recent illness outbreak associated with flour), and compare the thermal inactivation parameters (D- and z-values) of...
Thu, 11/07/2019 - 03:29
This study investigated the occurrence of shiga toxin‐producing Escherichia coli (STEC), thermotolerant Campylobacter spp. and Salmonella spp. in Swedish dairy milk. A total of 302 inline milk filters were analyzed. Salmonella was not isolated from any filters. Polymerase chain reaction screening detected thermotolerant Campylobacter in 30.5% of the milk filters analyzed and it was isolated from...
Wed, 10/30/2019 - 03:34
Author(s): E. Frank, R. Bonke, N. Drees, M. Heurich, E. Märtlbauer, M. GareisAbstractWorldwide infections by Shiga toxin-producing Escherichia coli (STEC) in humans have been reported after consumption of mainly beef, but also deer meat. Not only the consumption of contaminated deer meat represents a risk, but also the transmission of STEC between deer and domestic animals should be considered....
Sat, 10/26/2019 - 03:36
Shiga toxin‐producing Escherichia coli (STEC) is a zoonotic pathotype associated with human gastrointestinal disease that may progress to severe complications. Ruminants, especially cattle, are the main reservoirs of STEC from which they can contaminate the environment and foods of animal or vegetable origin. Besides Shiga toxin, other virulence factors are involved in STEC virulence. O157:H7...
Thu, 10/24/2019 - 03:43
Author(s): Anna Szczerba-Turek, Jan Siemionek, Piotr Socha, Agata Bancerz-Kisiel, Aleksandra Platt-Samoraj, Karolina Lipczynska-Ilczuk, Wojciech SzwedaABSTRACTShiga toxin-producing Escherichia (E.) coli (STEC) pathogens are responsible for the outbreaks of serious diseases in humans, including haemolytic uraemic syndrome (HUS), bloody diarrhoea (BD) and diarrhoea (D), and they pose a significant...
Fri, 10/11/2019 - 03:41
We report high-quality closed reference genomes for 1 bovine strain and 10 human Shiga toxin (Stx)-producing Escherichia coli (STEC) strains from serogroups O26, O45, O91, O103, O104, O111, O113, O121, O145, and O157. We also report draft assemblies, with standardized metadata, for 360 STEC strains isolated from watersheds, animals, farms, food, and human infections.
Fri, 10/04/2019 - 03:42
We present the complete genome sequence of an enterohemorrhagic Escherichia coli O111:H8 strain. This strain was isolated from a hemolytic-uremic syndrome patient and was responsible for a large outbreak associated with the consumption of raw beef in 2011.
Wed, 10/02/2019 - 03:41
Certain foodborne Shiga toxin-producing Escherichia coli (STEC) strains carry genes encoding the subtilase cytotoxin (SubAB). Although the mode of action of SubAB is under intensive investigation, information about the regulation of subAB gene expression is currently not available. In this study, we investigated the regulation of the chromosomal subAB1 gene in laboratory E. coli strain DH5...
Mon, 09/30/2019 - 17:10
Shiga toxin-producing Escherichia coli (STEC) O145 is one of the most prevalent non-O157 serogroups associated with foodborne outbreaks. Lytic phages are a potential alternative to antibiotics in combatting bacterial pathogens. In this study, we characterized a Siphoviridae phage lytic against STEC O145 strains as a novel antimicrobial agent. Escherichia phage vB_EcoS-Ro145clw (Ro145clw) was...
Thu, 09/12/2019 - 03:39
Author(s): Ronald Lulijwa, Andrea C. Alfaro, Fabrice Merien, Mark Burdass, Leonie Venter, Tim YoungAbstractWe investigated cellular functional and targeted immune cytokine responses of farmed Chinook salmon (Oncorhynchus tshawytscha) peripheral blood mononuclear cells (PBMCs) in vitro to LPS from Escherichia coli (E. coli) serotypes O111: B4 and O55: B5, and a phorbol ester phorbol 12-myristate...
Mon, 08/26/2019 - 01:05
Available online 25 August 2019 Author(s): Guerrino Macori, Siobhán C. McCarthy, Catherine M. Burgess, Séamus Fanning, Geraldine DuffyAbstractA quantitative PCR method is described for the detection and quantification of E. coli O157 and O26 in sheep recto-anal junction swabs. The method incorporated a short enrichment step (5 h) and the use of a developed standard calibration curve relating the...
Fri, 08/09/2019 - 15:58
Abstract Aims First, two inactivation models were compared for different phenotypic profiles of Escherichia coli O26 using ultraviolet‐C light (UV‐C) and thermal treatment (T), by means of Central Composite Rotatable Design of Experiment (CCRD). Second, we aimed to evaluate the subsequent survival and persistence of cells in simulated gastric fluid (SGF). Methods and Results Two strains of E....
Tue, 07/30/2019 - 17:00
Narjol González-Escalona, Marc A. Allard, Eric W. Brown, Shashi Sharma, Maria Hoffmann Whole genome sequencing can provide essential public health information. However, it is now known that widely used short-read methods have the potential to miss some randomly-distributed segments of genomes. This can prevent phages, plasmids, and virulence factors from being detected or properly identified....
Mon, 07/22/2019 - 05:03
Abstract Aims First, two inactivation models were compared for different phenotypic profiles of Escherichia coli O26 using UV‐C light (UV‐C) and thermal treatment (T), by means of Central Composite Rotatable Design of Experiment (CCRD). Second, we aimed to evaluate subsequent survival and persistence of cells in simulated gastric fluid (SGF). Methods and Results Two strains of E. coli O26, a...
Fri, 07/19/2019 - 00:00
Toxins, Vol. 11, Pages 424: Virulence Characteristics and Antimicrobial Resistance Profiles of Shiga Toxin-Producing Escherichia coli Isolates from Humans in South Africa: 2006–2013 Toxins doi: 10.3390/toxins11070424 Authors: Karama Cenci-Goga Malahlela Smith Keddy El-Ashram Kabiru Kalake Shiga toxin-producing Escherichia coli (STEC) isolates (N = 38) that were...
Thu, 07/18/2019 - 01:05
Available online 16 July 2019 Author(s): Magdalena Costa, Adriana Sucari, Sergio Epszteyn, Juan Oteiza, Jimena Gentiluomo, Celia Melamed, Yamila Figueroa, Santiago Mingorance, Agustina Grisaro, Silvia Spioussas, Mariana Buffoni Almeida, Mailen Caruso, Andrés Pontoni, Marcelo Signorini, Gerardo LeottaAbstractShiga toxin-producing Escherichia coli (STEC) are important pathogens transmitted by food...
Tue, 07/16/2019 - 03:00
Journal of Food Protection, Volume 82, Issue 8, Page 1308-1313, August 2019. Non-O157 Shiga toxin–producing Escherichia coli infections have recently been associated with wheat flour on two separate accounts in the United States and Canada. However, there is little information regarding the thermal resistance and longevity of non-O157 Shiga toxin–producing Escherichia coli during storage in low-...
Mon, 07/15/2019 - 01:05
Available online 14 July 2019 Author(s): Carlota Cedillo Martin, Cecilie Smith Svanevik, Bjørn Tore Lunestad, Camilla Sekse, Gro S. JohannessenAbstractOnly a few studies concerning Shiga toxin-producing E. coli (STEC) detection in bivalves and their harvesting areas have been reported, and to the best of our knowledge there are no outbreaks associated with STEC from bivalves described. The aim of...
Fri, 05/31/2019 - 01:12
Available online 29 May 2019 Author(s): Saida Essendoubi, Natisha Stashko, Iyla So, Gary Gensler, Deana Rolheiser, Chunu MainaliAbstractFrom January to December 2016, Alberta Agriculture and Forestry (AAF) conducted a provincial survey of selected pathogens and indicator organisms on beef carcasses processed at Provincially Licensed Abattoirs (PLAs) in Alberta. The survey was conducted in seven...

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