Enterohemorrhagic Escherichia coli

Fri, 03/27/2020 - 04:04
by Mariana Cap, Celeste Cingolani, Carla Lires, Marina Mozgovoj, Trinidad Soteras, Adriana Sucari, Jimena Gentiluomo, Adriana Descalzo, Gabriela Grigioni, Marcelo Signorini, Celina Horak, Sergio Vaudagna, Gerardo Leotta The aim of this study was to assess the efficacy of lactic acid (LA), caprylic acid (CA), high- (HDI) and low- (LDI) dose gamma irradiation and LDI combined with LA or CA on the...
Wed, 03/18/2020 - 03:33
This study examined the biofilm-forming ability of six non-O157 Shiga-toxin-producing Escherichia coli (STEC) strains: O116:H21, wzx-Onovel5:H19, O129:H21, O129:H23, O26:H11, and O154:H10 on stainless steel coupons after 24, 48, and 72 h of incubation at 22 °C and after 168 h at 10 °C. The results of crystal violet staining revealed that strains O129:H23 and O154:H10 were able to form biofilms on...
Fri, 03/06/2020 - 04:07
Shiga toxin-producing Escherichia coli (STEC) is a foodborne disease with worldwide outbreaks. STEC serotypes O157, O26, O45, O103, O111, O121, and O145 cause the most outbreaks. There is little published information regarding the other serotypes. We report the draft genome sequences for 11 uncommon STEC serotypes from Nebraska.
Wed, 03/04/2020 - 01:18
Recent outbreaks traced to contaminated flour have created a need in the milling industry for a process that reduces pathogens in wheat while maintaining its functional properties. Vacuum steam treatment is a promising technology for treatment of low moisture foods. Traditional thermal treatment methods can compromise wheat functionality due to high temperatures, thus maintaining the functional...
Tue, 02/25/2020 - 03:41
Escherichia coli serogroups O157, O26, O45, O103, O111, O121, and O145, when carrying major virulence genes, the Shiga toxin genes stx1 and stx2 and the intimin gene eae, are important foodborne pathogens. They are referred to as the "top 7" Shiga toxin-producing E. coli (STEC) serogroups and were declared by the USDA as adulterants to human health. Since top 7 serogroup-positive cattle feces and...
Fri, 02/21/2020 - 03:52
Extended-spectrum β-lactamase (ESBL)-producing bacteria constitute an emerging global health issue with food products being vehicles of transmission and the aquatic environments serving as potential reservoirs. This study aimed to characterize ESBL-producing Escherichia coli in Nile perch and water from Lake Victoria in Tanzania. A total of 180 samples of Nile perch and 60 water samples were...
Wed, 02/12/2020 - 10:50
Author(s): Marina C. Lamparter, Annica Seemann, Carolin Hobe, Elisabeth SchuhAbstractShiga toxin-producing Escherichia coli (STEC) in sprouts have caused large scale outbreaks in the past involving severe illness. The combination of this very diverse pathogen and a food matrix with high numbers of background microbiota poses a particular challenge for detection and isolation. An acid treatment of...
Wed, 01/22/2020 - 03:35
Current California agricultural practices strive to comanage food safety and habitat conservation on farmland. However, the ecology of foodborne pathogens in wild bird populations, especially those avian species residing in proximity to fresh produce production fields, is not fully understood. In this repeated cross-sectional study, avifauna within agricultural lands in California were sampled...
Tue, 01/21/2020 - 03:23
aEPEC are associated with persistent diarrhea, and diarrheal outbreaks in both humans and animals worldwide. They are differentiated from typical EPEC by the lack of bundle-forming pili, and from EHEC by the lack of phage-mediated stx toxins. However, phylogenetic analyses often associate aEPEC with EHEC, promoting the hypothesis that aEPEC are the progenitors of EHEC, which is supported by aEPEC...
Wed, 01/15/2020 - 03:23
Recent outbreaks traced to contaminated flour have created a need in the milling industry for a process that reduces pathogens in wheat while maintaining its functional properties. Vacuum steam treatment is a promising technology for treatment of low moisture foods. Traditional thermal treatment methods can compromise wheat functionality due to high temperatures, thus maintaining the functional...
Wed, 01/15/2020 - 03:23
One intrinsic characteristic of low-moisture foods that is frequently overlooked is pH. Although pH affects the survival of microorganisms in high-moisture foods, its influence in low-moisture foods with less available moisture has not been examined. Escherichia coli O157:H7, E. coli O121, Salmonella enterica Anatum, and S. enterica Agona were grown on solid media with and without added glucose,...
Tue, 12/31/2019 - 04:00
Author(s): Ayanna Glaize, Eduardo Gutierrez-Rodriguez, Irene Hanning, Sandra Díaz-Sánchez, Chris Gunter, Arnoud H.M. van Vliet, Wes Watson, Siddhartha ThakurAbstractThe interaction of typical host adapted enteric bacterial pathogens with fresh produce grown in fields is complex. These interactions can be more pronounced in co-managed or sustainable farms where animal operations are, by design,...
Tue, 12/24/2019 - 03:28
Shiga toxin‐producing Escherichia coli (STEC) can cause diarrhoea and severe diseases in humans, such as haemolytic uraemic syndrome. STEC virulence is considered to correlate with the amount of Shiga toxins (Stx) produced, especially Stx2, whose subtype Stx2a is most frequently associated with high virulence. Stx are encoded in prophages, which play an important role in STEC pathogenesis. The...
Thu, 12/12/2019 - 03:38
Author(s): Lin Chen, Xue Zhao, Ji'en Wu, Qin Liu, Xinyi Pang, Hongshun YangAbstractThe metabolic diversity of Escherichia coli strains (non-pathogenic E. coli ATCC 25922, and pathogenic E. coli O157:H7, O26:H11, O45:H2, O103:H11, O111, O121:H19, and O145) was tested using nuclear magnetic resonance. Based on two representative two-dimensional 1H-13C spectra, 38 metabolites were identified in E....
Wed, 11/20/2019 - 04:13
by Hiroaki Baba, Hajime Kanamori, Hayami Kudo, Yasutoshi Kuroki, Seiya Higashi, Kentaro Oka, Motomichi Takahashi, Makiko Yoshida, Kengo Oshima, Tetsuji Aoyagi, Koichi Tokuda, Mitsuo Kaku Shiga toxin-producing Escherichia coli (STEC) can cause severe gastrointestinal disease and colonization among food handlers. In Japan, STEC infection is a notifiable disease, and food handlers are required to...
Wed, 11/20/2019 - 04:05
Author(s): Alexander Gill, Tanis McMahon, Forest Dussault, Nicholas PetronellaAbstractWheat flour has recently been recognised as an exposure vehicle for the foodborne pathogen Shiga toxin-producing Escherichia coli (STEC). Wheat flour milled on two sequential production days in October 2016, and implicated in a Canada wide outbreak of STEC O121:H19, was analysed for the presence of STEC in...
Fri, 11/08/2019 - 03:34
Author(s): Minto Michael, Jennifer Acuff, Keyla Lopez, Daniel Vega, Randall Phebus, Harshavardhan Thippareddi, Lakshmikantha H. ChannaiahAbstractThis study was conducted to validate a simulated commercial baking process for plain muffins against E. coli O121 (isolated from the recent illness outbreak associated with flour), and compare the thermal inactivation parameters (D- and z-values) of...
Thu, 11/07/2019 - 03:29
This study investigated the occurrence of shiga toxin‐producing Escherichia coli (STEC), thermotolerant Campylobacter spp. and Salmonella spp. in Swedish dairy milk. A total of 302 inline milk filters were analyzed. Salmonella was not isolated from any filters. Polymerase chain reaction screening detected thermotolerant Campylobacter in 30.5% of the milk filters analyzed and it was isolated from...
Wed, 10/30/2019 - 03:34
Author(s): E. Frank, R. Bonke, N. Drees, M. Heurich, E. Märtlbauer, M. GareisAbstractWorldwide infections by Shiga toxin-producing Escherichia coli (STEC) in humans have been reported after consumption of mainly beef, but also deer meat. Not only the consumption of contaminated deer meat represents a risk, but also the transmission of STEC between deer and domestic animals should be considered....
Sat, 10/26/2019 - 03:36
Shiga toxin‐producing Escherichia coli (STEC) is a zoonotic pathotype associated with human gastrointestinal disease that may progress to severe complications. Ruminants, especially cattle, are the main reservoirs of STEC from which they can contaminate the environment and foods of animal or vegetable origin. Besides Shiga toxin, other virulence factors are involved in STEC virulence. O157:H7...
Thu, 10/24/2019 - 03:43
Author(s): Anna Szczerba-Turek, Jan Siemionek, Piotr Socha, Agata Bancerz-Kisiel, Aleksandra Platt-Samoraj, Karolina Lipczynska-Ilczuk, Wojciech SzwedaABSTRACTShiga toxin-producing Escherichia (E.) coli (STEC) pathogens are responsible for the outbreaks of serious diseases in humans, including haemolytic uraemic syndrome (HUS), bloody diarrhoea (BD) and diarrhoea (D), and they pose a significant...
Fri, 10/11/2019 - 03:41
We report high-quality closed reference genomes for 1 bovine strain and 10 human Shiga toxin (Stx)-producing Escherichia coli (STEC) strains from serogroups O26, O45, O91, O103, O104, O111, O113, O121, O145, and O157. We also report draft assemblies, with standardized metadata, for 360 STEC strains isolated from watersheds, animals, farms, food, and human infections.
Fri, 10/04/2019 - 03:42
We present the complete genome sequence of an enterohemorrhagic Escherichia coli O111:H8 strain. This strain was isolated from a hemolytic-uremic syndrome patient and was responsible for a large outbreak associated with the consumption of raw beef in 2011.
Wed, 10/02/2019 - 03:41
Certain foodborne Shiga toxin-producing Escherichia coli (STEC) strains carry genes encoding the subtilase cytotoxin (SubAB). Although the mode of action of SubAB is under intensive investigation, information about the regulation of subAB gene expression is currently not available. In this study, we investigated the regulation of the chromosomal subAB1 gene in laboratory E. coli strain DH5...
Mon, 09/30/2019 - 17:10
Shiga toxin-producing Escherichia coli (STEC) O145 is one of the most prevalent non-O157 serogroups associated with foodborne outbreaks. Lytic phages are a potential alternative to antibiotics in combatting bacterial pathogens. In this study, we characterized a Siphoviridae phage lytic against STEC O145 strains as a novel antimicrobial agent. Escherichia phage vB_EcoS-Ro145clw (Ro145clw) was...
Thu, 09/12/2019 - 03:39
Author(s): Ronald Lulijwa, Andrea C. Alfaro, Fabrice Merien, Mark Burdass, Leonie Venter, Tim YoungAbstractWe investigated cellular functional and targeted immune cytokine responses of farmed Chinook salmon (Oncorhynchus tshawytscha) peripheral blood mononuclear cells (PBMCs) in vitro to LPS from Escherichia coli (E. coli) serotypes O111: B4 and O55: B5, and a phorbol ester phorbol 12-myristate...
Mon, 08/26/2019 - 01:05
Available online 25 August 2019 Author(s): Guerrino Macori, Siobhán C. McCarthy, Catherine M. Burgess, Séamus Fanning, Geraldine DuffyAbstractA quantitative PCR method is described for the detection and quantification of E. coli O157 and O26 in sheep recto-anal junction swabs. The method incorporated a short enrichment step (5 h) and the use of a developed standard calibration curve relating the...
Fri, 08/09/2019 - 15:58
Abstract Aims First, two inactivation models were compared for different phenotypic profiles of Escherichia coli O26 using ultraviolet‐C light (UV‐C) and thermal treatment (T), by means of Central Composite Rotatable Design of Experiment (CCRD). Second, we aimed to evaluate the subsequent survival and persistence of cells in simulated gastric fluid (SGF). Methods and Results Two strains of E....
Tue, 07/30/2019 - 17:00
Narjol González-Escalona, Marc A. Allard, Eric W. Brown, Shashi Sharma, Maria Hoffmann Whole genome sequencing can provide essential public health information. However, it is now known that widely used short-read methods have the potential to miss some randomly-distributed segments of genomes. This can prevent phages, plasmids, and virulence factors from being detected or properly identified....
Mon, 07/22/2019 - 05:03
Abstract Aims First, two inactivation models were compared for different phenotypic profiles of Escherichia coli O26 using UV‐C light (UV‐C) and thermal treatment (T), by means of Central Composite Rotatable Design of Experiment (CCRD). Second, we aimed to evaluate subsequent survival and persistence of cells in simulated gastric fluid (SGF). Methods and Results Two strains of E. coli O26, a...
Fri, 07/19/2019 - 00:00
Toxins, Vol. 11, Pages 424: Virulence Characteristics and Antimicrobial Resistance Profiles of Shiga Toxin-Producing Escherichia coli Isolates from Humans in South Africa: 2006–2013 Toxins doi: 10.3390/toxins11070424 Authors: Karama Cenci-Goga Malahlela Smith Keddy El-Ashram Kabiru Kalake Shiga toxin-producing Escherichia coli (STEC) isolates (N = 38) that were...
Thu, 07/18/2019 - 01:05
Available online 16 July 2019 Author(s): Magdalena Costa, Adriana Sucari, Sergio Epszteyn, Juan Oteiza, Jimena Gentiluomo, Celia Melamed, Yamila Figueroa, Santiago Mingorance, Agustina Grisaro, Silvia Spioussas, Mariana Buffoni Almeida, Mailen Caruso, Andrés Pontoni, Marcelo Signorini, Gerardo LeottaAbstractShiga toxin-producing Escherichia coli (STEC) are important pathogens transmitted by food...
Tue, 07/16/2019 - 03:00
Journal of Food Protection, Volume 82, Issue 8, Page 1308-1313, August 2019. Non-O157 Shiga toxin–producing Escherichia coli infections have recently been associated with wheat flour on two separate accounts in the United States and Canada. However, there is little information regarding the thermal resistance and longevity of non-O157 Shiga toxin–producing Escherichia coli during storage in low-...
Mon, 07/15/2019 - 01:05
Available online 14 July 2019 Author(s): Carlota Cedillo Martin, Cecilie Smith Svanevik, Bjørn Tore Lunestad, Camilla Sekse, Gro S. JohannessenAbstractOnly a few studies concerning Shiga toxin-producing E. coli (STEC) detection in bivalves and their harvesting areas have been reported, and to the best of our knowledge there are no outbreaks associated with STEC from bivalves described. The aim of...
Fri, 05/31/2019 - 01:12
Available online 29 May 2019 Author(s): Saida Essendoubi, Natisha Stashko, Iyla So, Gary Gensler, Deana Rolheiser, Chunu MainaliAbstractFrom January to December 2016, Alberta Agriculture and Forestry (AAF) conducted a provincial survey of selected pathogens and indicator organisms on beef carcasses processed at Provincially Licensed Abattoirs (PLAs) in Alberta. The survey was conducted in seven...
Thu, 05/16/2019 - 11:01
Microcin PDI (MccPDI), a class IIa microcin that is produced by Escherichia coli strains 25 and 284, is known to inhibit foodborne pathogenic enterohemorrhagic E. coli serotypes O157:H7 and O26. Here we demonstrate that MccPDI can inhibit Shigella strains and E. coli isolates that are multidrug resistant, the latter including strains known to cause urinary tract infections in people and companion...
Tue, 05/14/2019 - 15:38
Abstract The present study was undertaken to detect the occurrence of beta‐lactamase and biofilm producing Enterobacteriaceae in healthy ducks. A total 202 cloacal swabs were collected from ducks kept in organized (n=92) and backyard (n=110) farms in West Bengal (India). The ducks had no history of antibiotic intake. Among the 87 phenotypically beta‐lactamase producing E. coli, 19 (17·43%), six (...
Tue, 04/16/2019 - 01:10
Available online 13 April 2019 Author(s): Minerva Rivera-Reyes, Jonathan A. Campbell, Catherine N. CutterAbstractShiga toxin-producing Escherichia coli (STEC) are known to adapt and survive acidic conditions. Acid-adaptation also may result in cross-protection against other stressors present in foods, such as reduced water activity (aw), refrigeration conditions, or the presence of antimicrobials...
Wed, 04/10/2019 - 01:14
Available online 9 April 2019 Author(s): M. Pesciaroli, J.E. Chardon, E.H.M. Delfgou, A.F.A. Kuijpers, L.M. Wijnands, E.G. EversAbstractMicrobial survival of heating and cross-contamination are the two transmission routes during food preparation in the consumers' kitchen that are relevant for QMRA (Quantitative Microbial Risk Assessment). The aim of the present study was to extend the limited...
Tue, 04/09/2019 - 20:00
O-Antigen Gene Clusters of Plesiomonas shigelloides Serogroups and Its Application in Development of a Molecular Serotyping Scheme Daoyi Xi1,2,3,4†, Xiaochen Wang1,2,3,4†, Kexin Ning1,2,3,4, Qian Liu1,2,3,4, Fuyi Jing1,2,3,4, Xi Guo1,2,3,4 and Boyang Cao1,2,3,4* 1Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, TEDA College, Nankai University...
Mon, 04/08/2019 - 20:00
A Comparative Analysis of the Metabolomic Response of Electron Beam Inactivated E. coli O26:H11 and Salmonella Typhimurium ATCC 13311 Sohini S. Bhatia and Suresh D. Pillai* National Center for Electron Beam Research, International Atomic Energy Agency (IAEA), Collaborating Centre for Electron Beam Technology, Texas A&M University, College Station, TX, United States Ionizing radiation...
Fri, 03/29/2019 - 01:11
LWT
Publication date: Available online 26 March 2019Source: LWTAuthor(s): Edson A. Rios, Jesús Santos, Isidro García-Meniño, Saskia C. Flament-Simon, Jorge Blanco, María-Luisa García-López, Andrés Otero, Jose M. Rodríguez-CallejaAbstractThis study was carried out to determine the occurrence and characteristics of enteropathogenic Escherichia coli (EPEC) and Shiga toxin-producing E. coli (STEC)...
Mon, 03/25/2019 - 16:31
Publication date: May 2019 Environmental Research, Volume 172Author(s): Joseph Haymaker, Manan Sharma, Salina Parveen, Fawzy Hashem, Eric B. May, Eric T. Handy, Chanelle White, Cheryl East, Rhodel Bradshaw, Shirley A. Micallef, Mary Theresa Callahan, Sarah Allard, Brienna Anderson, Shani Craighead, Samantha Gartley, Adam Vanore, Kalmia E. Kniel, Sultana Solaiman, Anthony Bui, Rianna...
Sun, 03/24/2019 - 01:12
Available online 22 March 2019 Author(s): Alexander Gill, Catherine Carrillo, Micaela Hadley, Robyn Kenwell, Linda ChuiAbstractA 2016/2017 outbreak of Shiga toxin-producing Escherichia coli (STEC) O121 in Canada, was linked to wheat flour, milled at a single facility on three consecutive days in October 2016. Most Probable Number (MPN) estimates of the concentration of STEC O121 in the recalled...
Thu, 03/07/2019 - 23:13
, Ahead of Print. Shiga toxin–producing Escherichia coli (STEC) serotype O121:H19 is one of the major non-O157:H7 serotypes associated with severe human disease. Here we examined population structure, virulence potential, and metabolic profile of environmental STEC O121 strains recovered from a major produce production region in California and performed comparative analyses with STEC O121...
Wed, 03/06/2019 - 11:00
We developed a multiplex PCR method based on multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) that was designed for the rapid typing of Escherichia coli and Shigella isolates. The method amplifies seven VNTRs and does not require a sequencing capillary or fluorescent dyes. The amplification products are simply loaded on a standard agarose gel for electrophoresis, and the...
Thu, 02/28/2019 - 01:10
Available online 26 February 2019 Author(s): Zeynal Topalcengiz, Michelle D. DanylukAbstractAgricultural water is considered as one of the main contamination source for produce prior to harvest. The purpose of study was to evaluate the fate of Shiga toxin-producing Escherichia coli (STEC), and generic E. coli in Central Florida agricultural surface water at different temperatures and the...
Fri, 02/22/2019 - 01:06
Fereidoun Forghani, Meghan den Bakker, Jye-Yin Liao, Alison S. Payton, Alexandra N. Futral, Francisco Diez-Gonzalez Salmonella and enterohemorrhagic Escherichia coli (EHEC) are of serious concern in wheat flour and its related products but little is known on their survival and thermal death kinetics. This study was undertaken to determine their long-term viability and thermal inactivation...
Thu, 02/21/2019 - 03:00
Journal of Food Protection, Volume 82, Issue 3, Page 395-404, March 2019. Shiga toxin–producing Escherichia coli (STEC) serogroups O26, O45, O103, O111, O121, and O145, referred to as the top six non-O157 serogroups, are responsible for more than 70% of human non-O157 STEC infections in North America. Cattle harbor non-O157 strains in the hindgut and shed them in the feces. The objective of this...
Thu, 01/31/2019 - 00:00
A. Browne et al. Shiga toxin–producing Escherichia coli serogroup O26 is an important public health pathogen. Phylogenetic bacterial lineages in a country can be associated with the level and timing of international imports of live cattle, the main reservoir. We sequenced the genomes of 152 E. coli O26 isolates from New Zealand and compared them with 252 E. coli O26 genomes from 14 other...

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