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Escherichia coli O157:H7

Thu, 07/18/2019 - 01:05
Available online 16 July 2019 Author(s): Anne-laure Moyne, Tyann Blessington, Thomas R. Williams, Steven T. Koike, Michael D. Cahn, Maria L. Marco, Linda J. HarrisAbstractThe impact of plant development, environmental conditions at the time of inoculation, and inoculum concentration on survival of attenuated BSL1 Escherichia coli O157:H7 strain ATCC 700728 on field-grown romaine lettuce was...
Wed, 07/17/2019 - 00:00
The inhibitory effect of lactose oxidase on the growth of foodborne pathogens and spoilage microorganisms associated with dairy products was evaluated through an overlay inhibition assay. Lactose oxidase generates hydrogen peroxide via lactose oxidation into lactobionic acid. Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enterica ser. Typhimurium, Staphylococcus aureus, Pseudomonas...
Tue, 07/16/2019 - 03:00
Journal of Food Protection, Volume 82, Issue 8, Page 1300-1307, August 2019.
Fri, 07/12/2019 - 01:04
Publication date: December 2019 , Volume 106Author(s): Yulin Zhu, Changzhu Li, Haiying Cui, Lin LinAbstractAs a non-thermal sterilization method, pulsed light (PL) has been widely applied in food industries to control the microbial contamination, however little is known about the antimicrobial mechanism of PL. This study deeply investigated the antimicrobial mechanism of PL against Escherichia...
Thu, 07/04/2019 - 00:00
Toxins, Vol. 11, Pages 392: A Short Peptide Derived from the ZorO Toxin Functions as an Effective Antimicrobial Toxins doi: 10.3390/toxins11070392 Authors: Otsuka Ishikawa Takahashi Masuda Antimicrobial peptides are potential molecules for the development of novel antibiotic agents. The ZorO toxin of a type I toxin–antitoxin system in Escherichia coli O157:H7 is...
Wed, 07/03/2019 - 13:08
Abstract A real‐time loop‐mediated isothermal amplification (Rti‐LAMP) DNA assay was developed for rapid and sensitive detection of viable Escherichia coli O157:H7 in milk by combining with ethidium monoazide bromide (EMA) and bentonite coated activated carbon (BCAC) treatment without enrichment. The assay involved inoculating 25 mL portions of milk with various numbers of E. coli O157:H7. Then...
Wed, 07/03/2019 - 13:08
Abstract A real‐time loop‐mediated isothermal amplification (Rti‐LAMP) DNA assay was developed for rapid and sensitive detection of viable Escherichia coli O157:H7 in milk by combining with ethidium monoazide bromide (EMA) and bentonite coated activated carbon (BCAC) treatment without enrichment. The assay involved inoculating 25 mL portions of milk with various numbers of E. coli O157:H7. Then...
Mon, 07/01/2019 - 11:32
In open environments such as water, enterohemorrhagic Escherichia coli O157:H7 responds to inorganic phosphate (Pi) starvation by inducing the Pho regulon controlled by PhoB. This activates the phosphate-specific transport (Pst) system that contains a high-affinity Pi transporter. In the pst mutant, PhoB is constitutively activated and regulates the expression of genes in the Pho regulon. Here we...
Mon, 07/01/2019 - 11:00
Essential oils (EOs) have demonstrated wide-spectrum antimicrobial activities and have been actively studied for their application in foods as alternative natural preservatives. However, information regarding microbial adaptive responses and changes in virulence properties following sublethal EO exposure is still scarce. The present study investigated the effect of sublethal thymol (Thy),...
Mon, 07/01/2019 - 01:05
Available online 29 June 2019 Author(s): Qisen Xiang, Wenjie Wang, Dianbo Zhao, Liyuan Niu, Ke Li, Yanhong BaiAbstractThis study was designed to evaluate the synergistic antimicrobial effect of plasma-activated water (PAW) combined with mild heat (40, 50, and 60 °C) against Escherichia coli O157:H7. Populations of E. coli O157:H7 were reduced from about 8.28 log CFU/mL to undetectable levels...
Mon, 07/01/2019 - 01:05
Available online 30 June 2019 Author(s): Linshu Lan, Ru Zhang, Xianglian Zhang, Hui ShiAbstractFoodborne pathogens can survive as the state of sublethal injury in adverse environments and recover physiological function and virulence under suitable conditions. Slightly acidic electrolyzed water (SAEW) is a non-thermal treatment to decontaminate in food industry and can induce sublethal injury....
Thu, 06/27/2019 - 01:07
Publication date: Available online 25 June 2019Source: LWTAuthor(s): Jingjing Ma, Huhu Wang, Lanlin Yu, Wenjing Yuan, Wenjing Fu, Feng Gao, Yun JiangAbstractThis study was aimed to investigate the dynamic recovery process of high pressure processing (HPP)-injured Escherichia coli O157:H7. E. coli O157:H7 was treated with 100–500 MPa for 5 min, and the pressure with highest injury rate was...
Thu, 06/27/2019 - 01:07
Available online 25 June 2019 Author(s): Hongchao Zhang, Heather Leigh Dolan, Qiao Ding, Siyuan Wang, Rohan V. TikekarAbstractWe investigated the antimicrobial efficacy of octanoic acid (OA) against Escherichia coli O157:H7 inoculated on the surface of baby spinach and grape tomatoes during simulated washing processes.3 mM OA at 45 °C achieved >6 log CFU/g reduction from the surface of...
Thu, 06/27/2019 - 01:07
Available online 25 June 2019 Author(s): Zijin Zhou, Sophie Zuber, Matteo Campagnoli, Thierry Putallaz, Frank Devlieghere, Mieke UyttendaeleAbstractThe microbial safety of black peppercorns (Piper nigrum L.) has raised concerns due to increasing numbers of reported foodborne outbreaks and food safety alerts. To avoid quality degradation caused by excessive thermal processing, mild steaming...
Mon, 06/24/2019 - 07:45
Abstract Rolling circle amplification (RCA) has been widely used to enhance detection signals as its long single‐stranded RCA products can provide multiple binding sites for signal probes for sensitive detections. In the current study, we employ atomic force microscopy (AFM) to monitor the RCA products during the course of the RCA process over time. Subsequently, the results of the RCA obtained...
Mon, 06/24/2019 - 07:45
Abstract Rolling circle amplification (RCA) has been widely used to enhance detection signals as its long single‐stranded RCA products can provide multiple binding sites for signal probes for sensitive detections. In the current study, we employ atomic force microscopy (AFM) to monitor the RCA products during the course of the RCA process over time. Subsequently, the results of the RCA obtained...
Sun, 06/23/2019 - 01:07
Available online 21 June 2019 Author(s): Emalie Thomas-Popo, Aubrey Mendonca, James Dickson, Angela Shaw, Shannon Coleman, Aura Daraba, Armitra Jackson-Davis, Floyd WoodsAbstractThe antimicrobial effectiveness of isoeugenol against Escherichia coli O157:H7, Salmonella enterica, and Listeria monocytogenes in refrigerated (4 °C) pineapple juice (PJ) with added 0.5% (w/v) yucca extract (YEX) was...
Fri, 06/21/2019 - 01:06
Available online 19 June 2019 Author(s): Qingyang Wang, Robert L. Buchanan, Rohan V. TikekarAbstractAbility of bacteria to develop cross-protection to various stresses is well-known. However, exploration into cross-protection between conventional physiological stresses and emerging antimicrobial treatments is limited. Previously, we developed two new antimicrobial treatments based on a combined...
Tue, 06/18/2019 - 13:35
Publication date: Available online 18 June 2019 Food BioscienceAuthor(s): Seoung Hyuk Kim, Wang June Kim, Seok-Seong KangAbstractThe inhibitory effect of Lactobacillus brevis DF01 and Pediococcus acidilactici K10, which had been isolated from kimchi, on enteropathogenic bacterial adhesion to intestinal epithelial cells as well as their probiotic potential was studied. After the incubation of Lb....
Sat, 06/15/2019 - 01:07
Available online 13 June 2019 Author(s): Qin Liu, Xiaoxuan Jin, Xiao Feng, Hongshun Yang, Caili FuAbstractIn the present study, the synergistic disinfection efficacy of low concentration electrolyzed water (LcEW) (free available chlorine, 4 mg/L) combined with brief heat enhancement was evaluated and the bactericidal mechanism was investigated by atomic force microscopy (AFM). The inactivation...
Thu, 06/13/2019 - 01:17
Available online 12 June 2019 Author(s): Lin Lin, Xinlei Wang, Haiying CuiAbstractPulsed magnetic fields (PMF) as a non-thermal sterilization has broad application prospects, but at this stage its bactericidal effect is not satisfactory. The essential oils have been proved with good antimicrobial effects. However, they will cover the original flavor of the food products at high dosages. In order...
Mon, 06/10/2019 - 01:13
Available online 8 June 2019 Author(s): Abdelrahim Hussein Abdelazeem Hassan, José Francisco Bergua, Eden morales-Narváez, Arben MekoçiAbstractConsidering the health risks of E. coli O157:H7 presence in food and water, an affordable and highly sensitive detection method is crucial. Herein, we report the first use of a single antibody-based fluorescent lateral flow immunoassay (FLFIA) depending on...
Thu, 05/30/2019 - 01:13
Publication date: September 2019 , Volume 123Author(s): Myrsini Kakagianni, Konstantinos P. KoutsoumanisAbstractA predictive mathematical model of the effect of temperature (10–47 °C) on the growth of Escherichia coli O157:H7 in natural contaminated ground beef was developed. The estimated values for the cardinal parameters Tmin, Tmax, Topt and the optimum maximum specific growth rate (μopt) of E...
Mon, 05/27/2019 - 01:11
Available online 25 May 2019 Author(s): Susana de Oliveira Elias, Tiago Baptista Noronha, Eduardo Cesar TondoAbstractLettuce (Lactuca sativa), one of the most consumed leafy vegetables in the world, is frequently implicated with foodborne disease (FBD) outbreaks, with Salmonella spp. and Escherichia coli O157:H7 being the most common bacteria to cause this illness. Estimates of prevalence and...
Sun, 05/26/2019 - 11:56
Abstract The bacteriological quality and safety of ready‐to‐eat (RTE) foods in Barbados were assessed in two separate studies conducted between 2014 and 2016. In Study 1, 206 food samples were processed for Salmonella spp. by conventional bacteriological methods. Polymerase chain reaction (PCR) and serotyping were used for confirmation. In Study 2, 120 food samples were processed for the total...
Sun, 05/26/2019 - 11:56
Abstract The bacteriological quality and safety of ready‐to‐eat (RTE) foods in Barbados were assessed in two separate studies conducted between 2014 and 2016. In Study 1, 206 food samples were processed for Salmonella spp. by conventional bacteriological methods. Polymerase chain reaction (PCR) and serotyping were used for confirmation. In Study 2, 120 food samples were processed for the total...
Sun, 05/26/2019 - 01:12
Available online 25 May 2019 Author(s): Berna Ozturk, Ilkin Yucel SengunAbstractThe aim of this study was to determine the effects of marination liquids prepared with koruk juice and dried koruk pomace in terms of meat safety. In the study, marination liquids containing two different concentrations of koruk juice (25% and 50%) or dried koruk pomace (1% and 2%) were prepared separately with or...
Fri, 05/24/2019 - 01:13
Available online 22 May 2019 Author(s): Xiaojing Tian, Lele Shao, Qianqian Yu, Huan Yang, Xingmin Li, Ruitong DaiAbstractIn this study, the effects of ohmic cooking (OH) and water bath cooking (WB) on the reduction of Escherichia coli O157:H7 inoculated in pork batter with addition of sodium chloride (NaCl) were studied, and the recovery and growth of OH and WB treated E. coli O157:H7 were also...
Tue, 05/21/2019 - 10:18
Abstract Escherichia coli O157:H7 (E coli O157:H7) is a highly virulent bacterial strain that is present in food, and as few as 10 cells can cause severe diseases. Rapid and sensitive bacterial detection is urgently required. In this study, we report a flow cytometry (FCM)‐based method for detecting E coli O157:H7 at the single‐cell level. Single E coli O157:H7 cells were obtained by a flow...
Tue, 05/21/2019 - 10:18
Abstract Escherichia coli O157:H7 (E coli O157:H7) is a highly virulent bacterial strain that is present in food, and as few as 10 cells can cause severe diseases. Rapid and sensitive bacterial detection is urgently required. In this study, we report a flow cytometry (FCM)‐based method for detecting E coli O157:H7 at the single‐cell level. Single E coli O157:H7 cells were obtained by a flow...
Thu, 05/16/2019 - 20:00
A total of forty-four lactic acid bacteria (LAB) strains originally isolated from cattle feces and different food sources were screened for their potential probiotic features. The antimicrobial activity of all isolates was tested by well-diffusion assay and competitive exclusion on broth against Salmonella Montevideo, Escherichia coli O157:H7 and Listeria monocytogenes strain N1-002. Thirty-eight...
Wed, 05/08/2019 - 01:15
Publication date: Available online 7 May 2019Source: LWTAuthor(s): Hang Qi, Yen-Con HungAbstractThis study was aimed to elucidate the mechanism of ferrous and alkaline activated persulfate in inactivating Escherichia coli O157:H7 and Listeria monocytogenes. A five-strain cocktail of each pathogen was treated with activated persulfate for 5 min. The treated pathogens were plated on both selective...
Sat, 05/04/2019 - 15:44
Abstract Aims Greenhouse trials were conducted with different cultivars of baby leaf spinach, rocket, and Swiss chard and inoculation of Escherichia coli O157:H7 gfp+, to determine whether plant species and cultivar has an impact on establishment of this strain. Methods and results Three cultivars each of spinach, rocket and Swiss chard were spray inoculated with E. coli O157:H7 gfp+ at doses...
Mon, 04/29/2019 - 01:12
Publication date: Available online 27 April 2019Source: LWTAuthor(s): George Kwabena Afari, Haijie Liu, Yen-Con HungAbstractThe effects of pathogen inoculum concentration, pH and organic load of electrolyzed oxidizing (EO) water treatment on the culturability and viability of Listeria monocytogenes and Escherichia coli O157:H7 was examined. Inoculum concentrations (8, 6, and 4 log CFU/mL), EO...
Fri, 04/26/2019 - 01:13
Publication date: August 2019Source: LWT, Volume 110Author(s): Lele Shao, Xiaojing Tian, Qianqian Yu, Lei Xu, Xingmin Li, Ruitong DaiAbstractIn order to explore the inactivation effect of ohmic heating on bacteria, inactivation and recovery kinetics of Escherichia coli O157:H7 treated with ohmic heating in broth were studied and compared with conventional water bath heating. During the heating...
Wed, 04/17/2019 - 20:00
The serious human pathogen, E. coli serotype O157:H7, continues to gain antibiotic resistance, posing a public health threat. While this serotype’s genome has been sequenced, the role of 25% of its genes remains ...
Mon, 04/08/2019 - 20:00
Gamma Irradiation Influences the Survival and Regrowth of Antibiotic-Resistant Bacteria and Antibiotic-Resistance Genes on Romaine Lettuce Vaishali Dharmarha1, Giselle Guron2, Renee R. Boyer1, Brendan A. Niemira3, Amy Pruden2, Laura K. Strawn1 and Monica A. Ponder1* 1Department of Food Science and Technology, Virginia Tech, Blacksburg, VA, United States 2Department of Civil and Environmental...
Fri, 04/05/2019 - 01:14
Available online 2 April 2019 Author(s): S. Ryu, S. Oh, M.R. Park, W.J. Lee, B. Yun, H.J. Choi, M.H. Oh, N.S. Oh, M.H. Song, Y. KimAbstractFoodborne pathogens, including enterohemorrhagic Escherichia coli (EHEC) O157:H7, may enter from the farm environment and foods via several different vectors and influence human health. Here, we employed Caenorhabditis elegans as a host model system and...
Wed, 04/03/2019 - 01:15
Available online 1 April 2019 Author(s): Sang-Soon Kim, Jae-Ik Lee, Dong-Hyun KangAbstractSalsa is a liquid-solid food containing jalapeño and serrano peppers, which result in multistate outbreak in 2008. Storage temperature and sodium chloride (NaCl) concentration of salsa vary depending on the climate, season, and type of product. In this regard, effect of growth conditions, namely, low...
Wed, 03/27/2019 - 01:14
Publication date: Available online 24 March 2019Source: LWTAuthor(s): Daizy Hwang, Elizabeth White, Anuj Purohit, Anand Mohan, Abhinav MishraAbstractSurvival of pathogenic bacteria under acidic conditions may pose a significant food safety challenge and should be assessed when developing new food products. The objective of this study was to determine the antimicrobial effect of two acidulants...
Tue, 03/26/2019 - 14:32
Abstract Aims The purpose of this research was to determine optimum conditions for broth recovery of heat‐injured Salmonella Typhimurium and Escherichia coli O157:H7. Methods and Results Exposure to 55°C for 15 and 25 min, respectively, induced cellular injury to those pathogens. Comparison was made with the commonly used overlay method using selective medium for recovering sub‐lethally...
Thu, 03/21/2019 - 01:18
Available online 19 March 2019 Author(s): Serajus Salaheen, Seon Woo Kim, Jeffrey S. Karns, Ernest Hovingh, Bradd J. Haley, Jo Ann S. Van KesselAbstractEscherichia coli O157:H7 (O157:H7) is a major zoonotic pathogen responsible for severe and sometimes fatal infections in humans. Cattle are the primary reservoir of O157:H7, although other animal species are also known carriers. Fecal shedding of...

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