Escherichia coli O157:H7

Thu, 02/13/2020 - 03:40
The Ib-M6 peptide has antibacterial activity against non-pathogenic Escherichia coli K-12 strain. The first part of this study determines the antibacterial activity of Ib-M6 against fourteen pathogenic strains of E. coli O157:H7. Susceptibility assay showed that Ib-M6 had values of Minimum Inhibitory Concentration (MIC) lower than streptomycin, used as a reference antibiotic. Moreover, to predict...
Fri, 02/07/2020 - 03:53
Aims The purpose of this study was to isolate Lactobacillus from gastrointestinal tract of healthy post‐weaning piglets and investigate its synergistic antimicrobial and probiotic effects with ZnO nanoparticles (nZnO). Methods and Results Out of the 128 isolates, L. plantarum BLPL03 was selected based on its excellent acid and bile salt tolerance properties. L. plantarum BLPL03 was sensitive to β...
Thu, 02/06/2020 - 03:33
LWT
Author(s): Atefeh Sadat Kashfi, Yousef Ramezan, Mohammad Reza KhaniAbstractThis study purpose was to investigate the effect of radiofrequency low pressure cold plasma (LPCP) process by using dried peppermint (Mentha piperita) on the reduction in microbial load, antioxidant activity, total phenolic content, and color. The microbial load of the sample was investigated by infecting it with 106 CFU/g...
Thu, 02/06/2020 - 03:30
The effects associated with fat content (0% to 40%) of pork batter and the positions (center and surface) on both ohmic heating (OH) and water bath heating (WB) treated samples, were investigated. The OH achieved a more uniform temperature distribution in pork batter as compared to WB‐ and OH‐treated samples showed lower cooking loss values for all samples tested. Besides, the thiobarbituric acid...
Fri, 01/31/2020 - 03:29
Salmonella enterica, Listeria monocytogenes, Shigella flexneri, Escherichia coli O157:H7, Vibrio parahaemolyticus, Staphylococcus aureus, Vibrio cholerae, Clostridium botulinum type A, Bacillus cereus, Clostridium perfringens Alpha toxin, and Yersinia enterocolitica are 11 common foodborne pathogens. Traditional bacterial culture methods for detecting pathogens are time‐consuming and labor‐...
Thu, 01/30/2020 - 03:25
Escherichia coli O157:H7 is an enteric foodborne pathogen associated with life threatening disease conditions. The enterobacteria are frequently found in cattle gastrointestinal tract with high potential of contaminating animal products such as meat, milk, and cheese. A cross-sectional study was conducted to investigate the presence of Shiga toxin-producing Escherichia coli O157:H7 in milk...
Thu, 01/23/2020 - 03:24
Cheeses made with unpasteurized milk are a safety concern due to possible contamination with foodborne pathogens. Listeria monocytogenes and Escherichia coli O157:H7 have been implicated in several outbreaks and recalls linked to Gouda cheese made with unpasteurized milk. The U.S. Food and Drug Administration Code of Federal Regulations requires cheeses made with unpasteurized milk to be aged at...
Fri, 01/17/2020 - 03:26
Process lethality during a 5 min resting period for E. coli O157 C37 and J3, and E. coli AW1.7 in patties cooked to 71°C. F2, single‐sided cooking with flipping twice; F3, single‐sided cooking with flipping three times; D, double‐sided cooking. Abstract This study assessed the adequacy of the current cooking recommendations in relation to heat resistant Escherichia coli by evaluating eight...
Wed, 01/15/2020 - 03:24
Enterohemorrhagic Escherichia coli (EHEC) strains are food-borne pathogens that can cause different clinical conditions. Shiga toxin 2a and/or 2c (Stx2)-producing E. coli O157:H7 is the serotype most frequently associated with severe human disease. In this work we analyzed the hypothesis that host cells participate in Stx2 production, cell damage, and inflammation during EHEC infection. With this...
Wed, 01/08/2020 - 03:40
Escherichia coli O157:H7 (EcO157) infections have been recurrently associated with produce. The physiological state of EcO157 cells surviving the many stresses encountered on plants is poorly understood. EcO157 populations on plants in the field generally follow a biphasic decay in which small subpopulations survive over longer periods of time. We hypothesized that these subpopulations include...
Wed, 01/08/2020 - 03:32
The apple‐, carrot‐, and hibiscus‐based edible films containing carvacrol and cinnamaldehyde showed antimicrobial effects against Escherichia coli O157:H7 on organic leafy greens in sealed plastic bags. All three types of 3% carvacrol‐containing films reduced E. coli O157:H7 population by about 5 log CFU/g at day 0. The 1.5% carvacrol‐containing films reduced E. coli O157:H7 population by 1–4...
Thu, 01/02/2020 - 03:29
The combined effect of the 222‐nm krypton‐chlorine (KrCl) excilamp and ohmic heating for the inactivation of Listeria monocytogenes, Salmonella enterica serovar Typhimurium, and Escherichia coli O157:H7 in apple juice was investigated in this study. When ohmic heating and a KrCl excilamp were applied to apple juice simultaneously, the reduction level of E. coli O157:H7 following 70 s (target...
Wed, 12/25/2019 - 03:23
Escherichia coli O157:H7 (STEC) acid resistance may aid the pathogen’s ability to cross the human gastric barrier and makes it an organism of concern in acidic foods. Our objective was to determine how STEC acid resistance may correlate with survival during vegetable fermentations. Seven E. coli O157:H7 strains were screened to assess acid resistance in simulated stomach-acid at pH 2. The...
Sat, 12/14/2019 - 03:30
Sand filter‐UV‐C treatment for irrigation water. Abstract Surface irrigation water is a food safety risk due to susceptibility to contamination and varying turbidity that affects chlorination efficacy. Effectiveness of a combined high‐throughput, commercially available sand filter and UV‐C system to inactivate Escherichia coli (K12, and attenuated O157:H7) in simulated irrigation water of varying...
Wed, 12/11/2019 - 04:00
Bacteriophages are a potential agent for the biocontrol of Shiga toxin‐producing Escherichia coli in milk. Stability and activity of bacteriophages may increase the safety of dairy products. Additionally, phages can successfully reduce bacterial biofilm on the food processing surface. Abstract Shiga toxin‐producing Escherichia coli is a common foodborne pathogen which transmission includes dairy...
Tue, 12/10/2019 - 03:28
Escherichia coli O157:H7 is an important food-borne pathogen and has ability to contaminate food, such as water, meat products, and milk. Moreover, E. coli O157:H7 is the main toxin-producing serotype which can produce Shiga toxin type 1 and type 2 and cause intestinal disease. The strong pathogenicity and lethality of Escherichia coli O157:H7 pose a serious threat to human. This study aims to...
Tue, 12/10/2019 - 03:28
The viable but non-culturable (VBNC) state, in which bacteria fail to grow on routine culture media but are actually alive, has been widely recognized as a strategy adopted by bacteria to cope with stressful environments. However, little is known regarding the molecular mechanism of VBNC formation. Here, we aimed to elucidate the specific roles of cell division regulatory proteins and the cell...
Sun, 12/08/2019 - 03:41
Two experiments were conducted to evaluate the feeding of direct fed microbials (DFMs) on fecal shedding of Escherichia coli O157:H7 in naturally infected cattle (experiment I) and on Salmonella in the feces and peripheral lymph nodes (PLNs) of experimentally infected cattle (experiment II). Thirty cattle, 10 per treatment, were used in each experiment. Treatments in experiment I consisted of a...
Fri, 12/06/2019 - 03:27
BACKGROUND The consumption of pesticides‐contaminated sour cherry as fruit or juice has become a major health concern, and thus, intensified the search for alternative processing technologies such as pulsed electric fields (PEF), ozone (O), and ultrasonication (US). The novelties of this experimental study for sour cherry juice were four‐fold: (1) to quantify the removal efficiency of the new...
Tue, 12/03/2019 - 03:45
Bactericidal effects of various concentrations of phenyllactic acid on Shiga toxin–producing Escherichia coli (STEC), including E. coli O157:H7, O26:H11, O103:H2, and O121:H19, and on Salmonella Typhimurium DT104 in pure culture and microplates assays were studied. Beef cuts were surface sprayed with phenyllactic acid or lactic acid for inactivation of E. coli O157:H7 and Salmonella Typhimurium....
Thu, 11/21/2019 - 03:25
Shiga toxin-producing Escherichia coli (STEC) strains are responsible for multiple clinical syndromes, including hemolytic uremic syndrome (HUS). E. coli O157:H7 is the most prevalent serotype associated with HUS and produces a variety of virulence factors being Stx2 the responsible of the most HUS severe cases. After intestinal colonization by STEC, Stx2 is released into the intestinal lumen,...
Tue, 11/19/2019 - 03:29
This research aims to evaluate the effect of a previously optimized high‐pressure processing (HPP) treatment on the vegetable smoothie stability during 28 days at 5 °C and on the inactivation of a contamination with Listeria monocytogenes and Escherichia coli O157:H7 surrogates during 12 days at 15 °C. HPP (630 MPa/6 min) reduced native microbiota to values below detection limit (DL = 1 log CFU/g...
Fri, 11/15/2019 - 03:36
Escherichia coli O157:H7 is a common food pathogen which has a serious effect on human health. We report here the complete genome sequence of Escherichia coli O157:H7 strain Al Ain, isolated from camel feces in the United Arab Emirates.
Thu, 11/14/2019 - 03:25
The objective of this study was to evaluate the efficacy of simultaneous UV-A and UV-B irradiation (UV-A+B) for inactivating Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes in both phosphate-buffered saline (PBS) and apple juice. A cocktail of the three pathogens was inoculated into PBS and apple juice, and then the suspensions were irradiated with UV lamps of 356 nm...
Wed, 11/13/2019 - 03:25
MraW is a 16S rRNA methyltransferase and plays a role in the fine-tuning of the ribosomal decoding center. It was recently found to contribute to the virulence of Staphylococcus aureus. In this study, we examined the function of MraW in Escherichia coli O157:H7 and found that the deletion of mraW led to decreased motility, flagellar production and DNA methylation. Whole-genome bisulfite...
Sun, 11/10/2019 - 03:26
Alginate‐based edible coating acts as a carrier enabling the sustained release of thyme oil to the surface of the fresh‐cut apples, inhibiting the growth of Listeria monocytogenes, Salmonella Typhimurium, Staphylococcus aureus and Escherichia coli O157:H7 inoculated onto fresh‐cut apples. Summary In this study, the inhibition of an alginate‐based edible coating (EC) containing thyme oil (0.05%, 0...
Sun, 11/03/2019 - 02:25
Enterohemorrhagic Escherichia coli O157:H7 (EHEC O157) causes bloody diarrhea and hemolytic-uremic syndrome. EHEC O157 encounters varied microenvironments during infection, and can efficiently adapt to these usin...
Thu, 10/24/2019 - 03:43
Author(s): Anna Szczerba-Turek, Jan Siemionek, Piotr Socha, Agata Bancerz-Kisiel, Aleksandra Platt-Samoraj, Karolina Lipczynska-Ilczuk, Wojciech SzwedaABSTRACTShiga toxin-producing Escherichia (E.) coli (STEC) pathogens are responsible for the outbreaks of serious diseases in humans, including haemolytic uraemic syndrome (HUS), bloody diarrhoea (BD) and diarrhoea (D), and they pose a significant...
Sat, 10/19/2019 - 03:30
Escherichia coli O157:H7 is the predominant cause of diarrhea-associated hemolytic uremic syndrome (HUS) worldwide. Its cardinal virulence traits are Shiga toxins, which are encoded by stx genes, the most common of which are stx1a, stx2a, and stx2c. The toxins these genes encode differ in their in vitro and experimental phenotypes, but the human population-level impact of these differences is...
Sat, 10/19/2019 - 03:30
This experiment determined the efficacy of using a novel aqueous ozone treatment as a spray chill intervention to reduce Escherichia coli O157:H7 on surfaces of fresh beef compared with traditional water spray chill. Cutaneous trunci muscles were obtained from a local beef cattle processing plant. Muscles were divided into sixteen 25-cm2 sections, and each section was individually inoculated with...
Thu, 10/17/2019 - 03:32
The cover image is based on the Original Article Rolling circle amplification and its application in microfluidic systems for E. coli O157:H7 detections by Xudong Cao, Yuqian Jiang, Zhenyu Qiu et al., https://doi.org/10.1111/jfs.12671
Thu, 10/17/2019 - 03:31
Food contact surfaces (FCS) in food processing facilities may become contaminated with a number of unwanted microorganisms, such as Listeria monocytogenes, Escherichia coli O157:H7, and Staphylococcus aureus. To reduce contamination and the spread of disease, these surfaces may be treated with sanitizers or have active antimicrobial components adhered to them. Although significant efforts have...
Tue, 10/15/2019 - 03:49
Escherichia coli O157:H7 is a foodborne pathogen, implicated in various multi-state outbreaks. It encodes Shiga toxin on a prophage, and Shiga toxin production is linked to phage induction. An E. coli strain, designated 0.1229, was identified that amplified Stx2a production when co-cultured with E. coli O157:H7 strain PA2. Growth of PA2 in 0.1229 cell-free supernatants had a similar effect, even...
Sat, 10/12/2019 - 03:34
Escherichia coli O157:H7 is an important foodborne pathogen and has been implicated in numerous food poisoning outbreaks worldwide. Although several microbiological and molecular methods have been developed to detect E. coli O157:H7, the difficulty to rapidly detect low levels of the foodborne bacteria persists. Here, the optimization of a filtration technique to concentrate and rapidly detect E...
Wed, 10/02/2019 - 03:36
Controlling the free chlorine (FC) availability in wash water during sanitization of fresh produce enhances our ability to reduce microbial levels and prevent cross‐contamination. However, maintaining an ideal concentration of FC that could prevent the risk of contamination within the wash system is still a technical challenge in the industry, indicating the need to better understand wash water...
Wed, 10/02/2019 - 03:35
In Turkey, legislation reduced the salt content in fresh cheeses from 10% to 6.5% in dry matter (DM). This study investigated the effect of salt reduction on the fate of acid‐ and salt‐adapted Escherichia coli O157:H7 and Listeria monocytogenes, as well as the textural quality of recombined nonfat cheese. The cheese was manufactured using milk protein concentrate (85% protein) and skim milk...
Mon, 09/30/2019 - 16:54
ABSTRACT Here we exploit the natural properties of a synthetic nanoparticle (NP) scaffold as a subunit vaccine against enterohemorrhagic Escherichia coli (EHEC). Two EHEC-specific immunogenic antigens, namely, LomW and EscC, either alone or in combination, were covalently linked on the surface of gold nanoparticles (AuNPs) and used to immunize mice prior to challenge with EHEC O157:H7 strain 86-...
Wed, 09/25/2019 - 03:26
Contaminated beef is a known vehicle of Escherichia coli O157:H7 infection, although more attention is given to the control of E. coli O157:H7 in ground, rather than whole-cut, beef products. In September 2012, an investigation was initiated at an Alberta, Canada, beef plant after the detection of E. coli O157:H7 in two samples of trim cut from beef originating from this plant. Later in September...
Sun, 09/22/2019 - 03:24
Aims The aim of this study was to evaluate the microbiological quality of commercially prepared RTE sushi by enumerating aerobic mesophilic bacteria (AMB) and thermotolerant coliforms (TC) and detecting Escherichia coli and Salmonella spp. An isolate was identified as E. coli O157:H7 which was evaluated for its virulence and antimicrobial resistance profiling as well as its ability to form...
Sat, 09/21/2019 - 03:29
The present study tested the antibacterial activity, expressed as minimum bactericidal concentration (MBC), of zinc oxide nanoparticles (ZnO‐NPs), copper oxide nanoparticles (CuO‐NPs) and their combination with or without rosemary, clove or cinnamon extract against Escherichia coli O157:H7 and Listeria monocytogenes. The NPs were characterized by scanning electron microscopy. The sizes of ZnO‐NPs...
Sat, 09/21/2019 - 03:27
Aims To investigate the effects of ohmic heating (OH) and water bath heating (WB) on the membrane permeability, membrane structure, intracellular organization and leakage of intracellular substances of Escherichia coli O157:H7 at the same inactivation level and at a heating temperature of 72°C. Methods and Results Flow cytometry analysis indicated that membrane permeability of E. coli O157:H7 by...
Thu, 09/19/2019 - 14:29
Publication date: March 2020 , Volume 109Author(s): Quan Wang, MengYao Long, CaiYun Lv, SiPei Xin, XianGan Han, Wei JiangAbstractA simple, rapid, highly sensitive, and quantitative fluorescence-based immunochromatographic test (ICT) was successfully developed for the determination of Escherichia coli O157:H7 in food samples. Europium (III) chelated nanoparticles (EuNPs) conjugated to monoclonal...
Wed, 09/11/2019 - 20:00
Cattle have been identified as a major reservoir of E. coli O157:H7 for human infection; the ecology of the organism in sheep and goats is less understood. This study was carried out to determine prevalence, sour...
Wed, 09/11/2019 - 01:05
Available online 9 September 2019 Author(s): Meera Surendran Nair, Fulin Ma, Patrick Lau, Indu Upadhyaya, Kumar VenkitanarayananAbstractThe study investigated the efficacy of two GRAS-status phytochemicals, mega-resveratrol (RV) and naringenin (NG) to inactivate Escherichia coli O157:H7 (EHEC) in apple cider. A five-strain mixture of EHEC (∼7 log CFU/ml) was inoculated into cider, followed by the...
Wed, 09/11/2019 - 01:05
LWT
Publication date: Available online 8 September 2019Source: LWTAuthor(s): Jin-Hee Kim, Sun Jung, Se-Wook OhAbstractMicrobial enrichment of bacteria is essential for detection because generally foodborne pathogens exist on food at very low concentrations. This study described a rapid and simple pretreatment method, combination of bacteria concentration and DNA concentration for detection E. coli...
Tue, 09/10/2019 - 06:55
Abstract A method combining surface‐enhanced Raman scattering (SERS) with a lateral flow strip (LFS) was developed for the quantitative and sensitive analysis of Escherichia coli O157:H7. AuMBA@Ag nanoparticles were prepared as SERS probes, and 4‐methylthiobenzoic acid (MBA) as a Raman reporter was inserted into the interior gap of the Au@Ag core‐shell nanoparticles, which replaced the Au...
Tue, 08/27/2019 - 11:13
Abstract This study was aimed to investigate the effects of lactic acid stress with acid adaptation on the survival and expression of virulence‐related genes in Escherichia coli O157:H7 (strains CICC21530, 89, 95, 112, 109, and J42). E. coli were subjected to acid adaption (pH 5.0) for 1–4 hr and then exposed to acid stress (pH 3.5) for 30–120 min. Results showed that exposure to pH 3.5...
Mon, 08/26/2019 - 20:00
Natural microcin J25 (MccJ25) represent promising alternatives to traditional antibiotics for the treatment of drug-resistant infections. However, little is known about the antibacterial activity of recombinant MccJ25 against foodborne pathogens. Here, the activity of recombinant MccJ25 was examined using a matrix of conditions in order to assess the efficacy of recombinant MccJ25 as a mitigation...
Fri, 08/23/2019 - 01:03
Available online 21 August 2019 Author(s): Gökhan Kürşad Incili, Ahmet Koluman, Ayşegül Aktüre, Ahmet AtaşalanAbstractEscherichia coli O157:H7 is one of the most important food-borne pathogens to threaten public health. Cultural methods are used as a gold standard while they are laborious and time-consuming. Loop-mediated isothermal amplification (LAMP) method is an alternative method that became...

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