Escherichia coli O157:H7

Wed, 10/02/2019 - 03:36
Controlling the free chlorine (FC) availability in wash water during sanitization of fresh produce enhances our ability to reduce microbial levels and prevent cross‐contamination. However, maintaining an ideal concentration of FC that could prevent the risk of contamination within the wash system is still a technical challenge in the industry, indicating the need to better understand wash water...
Wed, 10/02/2019 - 03:35
In Turkey, legislation reduced the salt content in fresh cheeses from 10% to 6.5% in dry matter (DM). This study investigated the effect of salt reduction on the fate of acid‐ and salt‐adapted Escherichia coli O157:H7 and Listeria monocytogenes, as well as the textural quality of recombined nonfat cheese. The cheese was manufactured using milk protein concentrate (85% protein) and skim milk...
Mon, 09/30/2019 - 16:54
ABSTRACT Here we exploit the natural properties of a synthetic nanoparticle (NP) scaffold as a subunit vaccine against enterohemorrhagic Escherichia coli (EHEC). Two EHEC-specific immunogenic antigens, namely, LomW and EscC, either alone or in combination, were covalently linked on the surface of gold nanoparticles (AuNPs) and used to immunize mice prior to challenge with EHEC O157:H7 strain 86-...
Sun, 09/22/2019 - 03:24
Aims The aim of this study was to evaluate the microbiological quality of commercially prepared RTE sushi by enumerating aerobic mesophilic bacteria (AMB) and thermotolerant coliforms (TC) and detecting Escherichia coli and Salmonella spp. An isolate was identified as E. coli O157:H7 which was evaluated for its virulence and antimicrobial resistance profiling as well as its ability to form...
Sat, 09/21/2019 - 03:29
The present study tested the antibacterial activity, expressed as minimum bactericidal concentration (MBC), of zinc oxide nanoparticles (ZnO‐NPs), copper oxide nanoparticles (CuO‐NPs) and their combination with or without rosemary, clove or cinnamon extract against Escherichia coli O157:H7 and Listeria monocytogenes. The NPs were characterized by scanning electron microscopy. The sizes of ZnO‐NPs...
Sat, 09/21/2019 - 03:27
Aims To investigate the effects of ohmic heating (OH) and water bath heating (WB) on the membrane permeability, membrane structure, intracellular organization and leakage of intracellular substances of Escherichia coli O157:H7 at the same inactivation level and at a heating temperature of 72°C. Methods and Results Flow cytometry analysis indicated that membrane permeability of E. coli O157:H7 by...
Thu, 09/19/2019 - 14:29
Publication date: March 2020 , Volume 109Author(s): Quan Wang, MengYao Long, CaiYun Lv, SiPei Xin, XianGan Han, Wei JiangAbstractA simple, rapid, highly sensitive, and quantitative fluorescence-based immunochromatographic test (ICT) was successfully developed for the determination of Escherichia coli O157:H7 in food samples. Europium (III) chelated nanoparticles (EuNPs) conjugated to monoclonal...
Wed, 09/11/2019 - 20:00
Cattle have been identified as a major reservoir of E. coli O157:H7 for human infection; the ecology of the organism in sheep and goats is less understood. This study was carried out to determine prevalence, sour...
Wed, 09/11/2019 - 01:05
Available online 9 September 2019 Author(s): Meera Surendran Nair, Fulin Ma, Patrick Lau, Indu Upadhyaya, Kumar VenkitanarayananAbstractThe study investigated the efficacy of two GRAS-status phytochemicals, mega-resveratrol (RV) and naringenin (NG) to inactivate Escherichia coli O157:H7 (EHEC) in apple cider. A five-strain mixture of EHEC (∼7 log CFU/ml) was inoculated into cider, followed by the...
Wed, 09/11/2019 - 01:05
LWT
Publication date: Available online 8 September 2019Source: LWTAuthor(s): Jin-Hee Kim, Sun Jung, Se-Wook OhAbstractMicrobial enrichment of bacteria is essential for detection because generally foodborne pathogens exist on food at very low concentrations. This study described a rapid and simple pretreatment method, combination of bacteria concentration and DNA concentration for detection E. coli...
Tue, 09/10/2019 - 06:55
Abstract A method combining surface‐enhanced Raman scattering (SERS) with a lateral flow strip (LFS) was developed for the quantitative and sensitive analysis of Escherichia coli O157:H7. AuMBA@Ag nanoparticles were prepared as SERS probes, and 4‐methylthiobenzoic acid (MBA) as a Raman reporter was inserted into the interior gap of the Au@Ag core‐shell nanoparticles, which replaced the Au...
Tue, 08/27/2019 - 11:13
Abstract This study was aimed to investigate the effects of lactic acid stress with acid adaptation on the survival and expression of virulence‐related genes in Escherichia coli O157:H7 (strains CICC21530, 89, 95, 112, 109, and J42). E. coli were subjected to acid adaption (pH 5.0) for 1–4 hr and then exposed to acid stress (pH 3.5) for 30–120 min. Results showed that exposure to pH 3.5...
Mon, 08/26/2019 - 20:00
Natural microcin J25 (MccJ25) represent promising alternatives to traditional antibiotics for the treatment of drug-resistant infections. However, little is known about the antibacterial activity of recombinant MccJ25 against foodborne pathogens. Here, the activity of recombinant MccJ25 was examined using a matrix of conditions in order to assess the efficacy of recombinant MccJ25 as a mitigation...
Fri, 08/23/2019 - 01:03
Available online 21 August 2019 Author(s): Gökhan Kürşad Incili, Ahmet Koluman, Ayşegül Aktüre, Ahmet AtaşalanAbstractEscherichia coli O157:H7 is one of the most important food-borne pathogens to threaten public health. Cultural methods are used as a gold standard while they are laborious and time-consuming. Loop-mediated isothermal amplification (LAMP) method is an alternative method that became...
Tue, 08/20/2019 - 01:05
Available online 17 August 2019 Author(s): Alejandra Orihuel, Lucrecia Terán, Jenny Renaut, Sébastien Planchon, María Pía Valacco, Emilse Masias, Carlos Minahk, Graciela Vignolo, Silvia Moreno, André M. De Almeida, Lucila Saavedra, Silvina FaddaAbstractThe enterohemorrhagic Escherichia (E.) coli (EHEC) is a pathogen of great concern for public health and the meat industry all over the world. The...
Sat, 08/17/2019 - 01:04
Available online 16 August 2019 Author(s): Jitendra Patel, Hsin-Bai Yin, Gary Bauchan, Joseph MoweryAbstractEscherichia coli O157:H7 and Salmonella enterica are foodborne pathogens with major public health concern in the U.S. These pathogens utilize several virulence factors to initiate infections in humans. The antimicrobial effect of seven glucosinolate hydrolysis compounds against Salmonella...
Thu, 08/15/2019 - 03:00
Journal of Food Protection, Volume 82, Issue 9, Page 1532-1538, September 2019.
Wed, 08/14/2019 - 11:00
This study investigated the microbial dynamics in multispecies biofilms of Escherichia coli O157:H7 strain 1934 (O157) or Salmonella enterica serovar Typhimurium ATCC 14028 (ST) and 40 strains of meat processing surface bacteria (MPB). Biofilms of O157 or ST with/without MPB were developed on stainless steel coupons at 15°C for up to 6 days. Bacteria in suspensions (inoculum, days 2 and 6)...
Tue, 08/13/2019 - 14:54
Abstract Aims To investigate the effects of ohmic heating (OH) and water bath heating (WB) on the membrane permeability, membrane structure, intracellular organization, and leakage of intracellular substances of E. coli O157:H7 at the same inactivation level and heating temperature of 72 °C. Methods and Results Flow cytometry (FCM) analysis indicated that membrane permeability of E. coli O157:...
Wed, 07/31/2019 - 01:03
LWT
Publication date: Available online 29 July 2019Source: LWTAuthor(s): Alex Tsun Yin Lau, Shai Barbut, Kelly Ross, Moussa Sory Diarra, S. BalamuruganAbstractThe effect of cranberry pomace extract (CE), on the growth of commonly used meat fermentation starter cultures, and selected foodborne pathogens were studied. Ten meat starter culture strains, belonging to genus Lactobacillus, Pediococcus, and...
Wed, 07/24/2019 - 00:00
This study determined the prevalence, pulsed-field gel electrophoresis profiles, and antimicrobial resistance profile of Listeria monocytogenes, Salmonella enterica, and Escherichia coli O157:H7 isolates from dairy cattle farms in Jordan. Samples from bulk tank milk (n = 305), cattle feces (n = 610), and rectoanal mucosal swabs (n = 610) were collected from 61 dairy cattle farms. We confirmed 32...
Thu, 07/18/2019 - 01:05
Available online 16 July 2019 Author(s): Anne-laure Moyne, Tyann Blessington, Thomas R. Williams, Steven T. Koike, Michael D. Cahn, Maria L. Marco, Linda J. HarrisAbstractThe impact of plant development, environmental conditions at the time of inoculation, and inoculum concentration on survival of attenuated BSL1 Escherichia coli O157:H7 strain ATCC 700728 on field-grown romaine lettuce was...
Wed, 07/17/2019 - 00:00
The inhibitory effect of lactose oxidase on the growth of foodborne pathogens and spoilage microorganisms associated with dairy products was evaluated through an overlay inhibition assay. Lactose oxidase generates hydrogen peroxide via lactose oxidation into lactobionic acid. Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enterica ser. Typhimurium, Staphylococcus aureus, Pseudomonas...
Tue, 07/16/2019 - 03:00
Journal of Food Protection, Volume 82, Issue 8, Page 1300-1307, August 2019.
Fri, 07/12/2019 - 01:04
Publication date: December 2019 , Volume 106Author(s): Yulin Zhu, Changzhu Li, Haiying Cui, Lin LinAbstractAs a non-thermal sterilization method, pulsed light (PL) has been widely applied in food industries to control the microbial contamination, however little is known about the antimicrobial mechanism of PL. This study deeply investigated the antimicrobial mechanism of PL against Escherichia...
Thu, 07/04/2019 - 00:00
Toxins, Vol. 11, Pages 392: A Short Peptide Derived from the ZorO Toxin Functions as an Effective Antimicrobial Toxins doi: 10.3390/toxins11070392 Authors: Otsuka Ishikawa Takahashi Masuda Antimicrobial peptides are potential molecules for the development of novel antibiotic agents. The ZorO toxin of a type I toxin–antitoxin system in Escherichia coli O157:H7 is...
Wed, 07/03/2019 - 13:08
Abstract A real‐time loop‐mediated isothermal amplification (Rti‐LAMP) DNA assay was developed for rapid and sensitive detection of viable Escherichia coli O157:H7 in milk by combining with ethidium monoazide bromide (EMA) and bentonite coated activated carbon (BCAC) treatment without enrichment. The assay involved inoculating 25 mL portions of milk with various numbers of E. coli O157:H7. Then...
Mon, 07/01/2019 - 11:32
In open environments such as water, enterohemorrhagic Escherichia coli O157:H7 responds to inorganic phosphate (Pi) starvation by inducing the Pho regulon controlled by PhoB. This activates the phosphate-specific transport (Pst) system that contains a high-affinity Pi transporter. In the pst mutant, PhoB is constitutively activated and regulates the expression of genes in the Pho regulon. Here we...
Mon, 07/01/2019 - 11:00
Essential oils (EOs) have demonstrated wide-spectrum antimicrobial activities and have been actively studied for their application in foods as alternative natural preservatives. However, information regarding microbial adaptive responses and changes in virulence properties following sublethal EO exposure is still scarce. The present study investigated the effect of sublethal thymol (Thy),...
Mon, 07/01/2019 - 01:05
Available online 29 June 2019 Author(s): Qisen Xiang, Wenjie Wang, Dianbo Zhao, Liyuan Niu, Ke Li, Yanhong BaiAbstractThis study was designed to evaluate the synergistic antimicrobial effect of plasma-activated water (PAW) combined with mild heat (40, 50, and 60 °C) against Escherichia coli O157:H7. Populations of E. coli O157:H7 were reduced from about 8.28 log CFU/mL to undetectable levels...
Mon, 07/01/2019 - 01:05
Available online 30 June 2019 Author(s): Linshu Lan, Ru Zhang, Xianglian Zhang, Hui ShiAbstractFoodborne pathogens can survive as the state of sublethal injury in adverse environments and recover physiological function and virulence under suitable conditions. Slightly acidic electrolyzed water (SAEW) is a non-thermal treatment to decontaminate in food industry and can induce sublethal injury....
Thu, 06/27/2019 - 01:07
LWT
Publication date: Available online 25 June 2019Source: LWTAuthor(s): Jingjing Ma, Huhu Wang, Lanlin Yu, Wenjing Yuan, Wenjing Fu, Feng Gao, Yun JiangAbstractThis study was aimed to investigate the dynamic recovery process of high pressure processing (HPP)-injured Escherichia coli O157:H7. E. coli O157:H7 was treated with 100–500 MPa for 5 min, and the pressure with highest injury rate was...
Thu, 06/27/2019 - 01:07
Available online 25 June 2019 Author(s): Hongchao Zhang, Heather Leigh Dolan, Qiao Ding, Siyuan Wang, Rohan V. TikekarAbstractWe investigated the antimicrobial efficacy of octanoic acid (OA) against Escherichia coli O157:H7 inoculated on the surface of baby spinach and grape tomatoes during simulated washing processes.3 mM OA at 45 °C achieved >6 log CFU/g reduction from the surface of...
Thu, 06/27/2019 - 01:07
Available online 25 June 2019 Author(s): Zijin Zhou, Sophie Zuber, Matteo Campagnoli, Thierry Putallaz, Frank Devlieghere, Mieke UyttendaeleAbstractThe microbial safety of black peppercorns (Piper nigrum L.) has raised concerns due to increasing numbers of reported foodborne outbreaks and food safety alerts. To avoid quality degradation caused by excessive thermal processing, mild steaming...
Mon, 06/24/2019 - 07:45
Abstract Rolling circle amplification (RCA) has been widely used to enhance detection signals as its long single‐stranded RCA products can provide multiple binding sites for signal probes for sensitive detections. In the current study, we employ atomic force microscopy (AFM) to monitor the RCA products during the course of the RCA process over time. Subsequently, the results of the RCA obtained...
Sun, 06/23/2019 - 01:07
Available online 21 June 2019 Author(s): Emalie Thomas-Popo, Aubrey Mendonca, James Dickson, Angela Shaw, Shannon Coleman, Aura Daraba, Armitra Jackson-Davis, Floyd WoodsAbstractThe antimicrobial effectiveness of isoeugenol against Escherichia coli O157:H7, Salmonella enterica, and Listeria monocytogenes in refrigerated (4 °C) pineapple juice (PJ) with added 0.5% (w/v) yucca extract (YEX) was...
Fri, 06/21/2019 - 01:06
Available online 19 June 2019 Author(s): Qingyang Wang, Robert L. Buchanan, Rohan V. TikekarAbstractAbility of bacteria to develop cross-protection to various stresses is well-known. However, exploration into cross-protection between conventional physiological stresses and emerging antimicrobial treatments is limited. Previously, we developed two new antimicrobial treatments based on a combined...
Tue, 06/18/2019 - 13:35
Publication date: Available online 18 June 2019 Food BioscienceAuthor(s): Seoung Hyuk Kim, Wang June Kim, Seok-Seong KangAbstractThe inhibitory effect of Lactobacillus brevis DF01 and Pediococcus acidilactici K10, which had been isolated from kimchi, on enteropathogenic bacterial adhesion to intestinal epithelial cells as well as their probiotic potential was studied. After the incubation of Lb....
Sat, 06/15/2019 - 01:07
Available online 13 June 2019 Author(s): Qin Liu, Xiaoxuan Jin, Xiao Feng, Hongshun Yang, Caili FuAbstractIn the present study, the synergistic disinfection efficacy of low concentration electrolyzed water (LcEW) (free available chlorine, 4 mg/L) combined with brief heat enhancement was evaluated and the bactericidal mechanism was investigated by atomic force microscopy (AFM). The inactivation...
Thu, 06/13/2019 - 01:17
Available online 12 June 2019 Author(s): Lin Lin, Xinlei Wang, Haiying CuiAbstractPulsed magnetic fields (PMF) as a non-thermal sterilization has broad application prospects, but at this stage its bactericidal effect is not satisfactory. The essential oils have been proved with good antimicrobial effects. However, they will cover the original flavor of the food products at high dosages. In order...
Mon, 06/10/2019 - 01:13
Available online 8 June 2019 Author(s): Abdelrahim Hussein Abdelazeem Hassan, José Francisco Bergua, Eden morales-Narváez, Arben MekoçiAbstractConsidering the health risks of E. coli O157:H7 presence in food and water, an affordable and highly sensitive detection method is crucial. Herein, we report the first use of a single antibody-based fluorescent lateral flow immunoassay (FLFIA) depending on...
Thu, 05/30/2019 - 01:13
Publication date: September 2019 , Volume 123Author(s): Myrsini Kakagianni, Konstantinos P. KoutsoumanisAbstractA predictive mathematical model of the effect of temperature (10–47 °C) on the growth of Escherichia coli O157:H7 in natural contaminated ground beef was developed. The estimated values for the cardinal parameters Tmin, Tmax, Topt and the optimum maximum specific growth rate (μopt) of E...
Mon, 05/27/2019 - 01:11
Available online 25 May 2019 Author(s): Susana de Oliveira Elias, Tiago Baptista Noronha, Eduardo Cesar TondoAbstractLettuce (Lactuca sativa), one of the most consumed leafy vegetables in the world, is frequently implicated with foodborne disease (FBD) outbreaks, with Salmonella spp. and Escherichia coli O157:H7 being the most common bacteria to cause this illness. Estimates of prevalence and...
Sun, 05/26/2019 - 11:56
Abstract The bacteriological quality and safety of ready‐to‐eat (RTE) foods in Barbados were assessed in two separate studies conducted between 2014 and 2016. In Study 1, 206 food samples were processed for Salmonella spp. by conventional bacteriological methods. Polymerase chain reaction (PCR) and serotyping were used for confirmation. In Study 2, 120 food samples were processed for the total...
Sun, 05/26/2019 - 01:12
Available online 25 May 2019 Author(s): Berna Ozturk, Ilkin Yucel SengunAbstractThe aim of this study was to determine the effects of marination liquids prepared with koruk juice and dried koruk pomace in terms of meat safety. In the study, marination liquids containing two different concentrations of koruk juice (25% and 50%) or dried koruk pomace (1% and 2%) were prepared separately with or...
Fri, 05/24/2019 - 01:13
Available online 22 May 2019 Author(s): Xiaojing Tian, Lele Shao, Qianqian Yu, Huan Yang, Xingmin Li, Ruitong DaiAbstractIn this study, the effects of ohmic cooking (OH) and water bath cooking (WB) on the reduction of Escherichia coli O157:H7 inoculated in pork batter with addition of sodium chloride (NaCl) were studied, and the recovery and growth of OH and WB treated E. coli O157:H7 were also...
Tue, 05/21/2019 - 10:18
Abstract Escherichia coli O157:H7 (E coli O157:H7) is a highly virulent bacterial strain that is present in food, and as few as 10 cells can cause severe diseases. Rapid and sensitive bacterial detection is urgently required. In this study, we report a flow cytometry (FCM)‐based method for detecting E coli O157:H7 at the single‐cell level. Single E coli O157:H7 cells were obtained by a flow...
Thu, 05/16/2019 - 20:00
A total of forty-four lactic acid bacteria (LAB) strains originally isolated from cattle feces and different food sources were screened for their potential probiotic features. The antimicrobial activity of all isolates was tested by well-diffusion assay and competitive exclusion on broth against Salmonella Montevideo, Escherichia coli O157:H7 and Listeria monocytogenes strain N1-002. Thirty-eight...

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