Escherichia coli O157:H7

Wed, 10/21/2020 - 16:00
In December 2015, six cases of Shiga toxin (Stx)-producing Escherichia coli (STEC) O157:H7 stx2a/stx2c phage type (PT) 24 were identified by the national gastrointestinal disease surveillance system at Public Health England (PHE). Frozen grated coconut imported from India was implicated as the vehicle of infection. Short and long read sequencing data were interrogated for genomic markers to...
Fri, 09/11/2020 - 22:17
Aims To identify and evaluate the application of two novel monoclonal antibody (mAb) 2G12 against outer membrane protein (Omp) C and mAb 12B1 targeting the O chain of the lipopolysaccharides (LPS) of Escherichia coli O157:H7 (ECO157). Methods and Results The sensitivity and specificity of these two antibodies were evaluated with eight ECO157 strains and 68 untargeted strains. mAb 2G12 and 12B1...
Thu, 09/10/2020 - 08:04
L. Byrne et al. Signs and symptoms of Shiga toxin–producing Escherichia coli (STEC) serogroup O157:H7 infection range from mild gastrointestinal to bloody diarrhea and hemolytic uremic syndrome (HUS). We assessed the association between Shiga toxin gene (stx) subtype and disease severity for »3,000 patients with STEC O157:H7 in England during 2009–2019. Odds of bloody diarrhea, HUS, or both, were...
Wed, 09/09/2020 - 08:02
The objective of this study was to evaluate the effectiveness of a direct-fed microbial (DFM) product in reducing fecal shedding of Escherichia coli O157:H7 in finishing commercial feedlot cattle in Kansas (KS) and Nebraska (NE). Utilizing a randomized complete block design within the feedlot (KS, n = 1; NE, n = 1), cattle were randomly allocated to 20 pens, grouped in blocks of two based on...
Sun, 09/06/2020 - 22:17
Aim To study the impact of incorporating micro‐nano‐bubbles (MNBs) in commonly used food antimicrobials (AMs) against Escherichia coli O157:H7 (EC) and Listeria monocytogenes (LM). Methods and Results Air, carbon dioxide (CO2) and nitrogen (N2) were used to incorporate MNBs in city water. Nine‐milliliter AM solutions (with or without MNBs) were individually taken into sterile test‐tubes and mixed...
Tue, 08/18/2020 - 22:19
Escherichia coli O157:H7 entered into the Viable but Non Culturable (VBNC) state under salinity stress of 10 and 30% during production of salted silver carp. VBNC E. coli O157:H7 induced by NaCl stress during salted fish production continued to express its pathogenic genes of stx1, stx2, eae and hly. Abstract Many bacteria including Escherichia coli can enter into the viable but non‐culturable (...
Tue, 08/18/2020 - 22:19
A microfluidic device was applied to enumerate Escherichia coli O157:H7 cells in lettuce and beef samples. E. coli O157:H7 inoculated in lettuce and beef were collected with high efficiency by using filtration or two‐step centrifugation. This method could quantify target microbial cells within 1 hr. Abstract Quality assurance is one of the fundamental ways of preventing infections from foodborne...
Wed, 08/12/2020 - 22:20
Escherichia coli O157:H7, a causative agent of haemolytic uremic syndrome, can enter into a viable but non‐culturable (VBNC) state in response to harsh stress. Bacteria in this state can retain membrane integrity, metabolic activity and virulence expression, which may present health risks. However, virulence expression and resuscitation ability of the VBNC state are not well understood. Here, we...
Thu, 08/06/2020 - 22:14
Applications of whey protein concentrate (WPC) based films have been limited in the food industry due to their poor mechanical properties. This research aims to evaluate the effect of silver nanoparticles (AgNPs) synthesized by Aspergillus niger on the mechanical and antimicrobial properties of WPC based films. The biosynthesized AgNPs solution was added into the WPC film formula at the...
Fri, 07/31/2020 - 16:00
Agricultural soil contaminated by manure is becoming an important source for the transmission of foodborne pathogens. There is an urgent need for a rapid and accurate method for viable pathogen detection in agricultural soil samples. Propidium monoazide (PMA) is a DNA-binding dye that can inhibit the amplification of DNA from dead cells through subsequent quantitative polymerase chain reaction (...
Thu, 07/16/2020 - 10:02
Several outbreaks of Escherichia coli O157:H7 associated with contaminated leafy green vegetables have been documented. Here, we report the draft genome sequences of 14 strains isolated from human patients in the state of Wisconsin during a multistate outbreak in early 2018 that was linked to consumption of romaine lettuce.
Wed, 07/08/2020 - 22:16
Efficacy of N‐Halamine on Foodborne Pathogens Abstract A major concern of the cattle industry is cross‐contamination of meat with pathogens. Cattle are exposed to fecal material, mud, and other contaminants which harbor pathogens that can be shed onto meat and meat processing equipment. Due to increased chances of meat contamination during processing, new antimicrobial formulations for carcass...
Wed, 07/08/2020 - 00:00
Antimicrobial peptides constitute an excellent alternative against conventional antibiotics because of their potent antimicrobial spectrum, unspecific action mechanism and low capacity to produce antibiotic resistance. However, a potential use of these biological molecules as therapeutic agents is threatened by their low stability and susceptibility to proteases. In order to overcome these...
Sat, 06/27/2020 - 08:02
Escherichia coli O157:H7 is an extremely serious foodborne pathogen accounting for a vast number of hospitalizations. In this system, a simple, rapid, and safe compound method was developed based on carbonyl iron powder (CIP) and multiwalled carbon nanotubes (MWCNT). Then, the CIP@MWCNT-based aptasensor was constructed by strong π-stacking between nanocomposite and aptamer, single-strand DNA,...
Fri, 06/26/2020 - 04:02
ABSTRACT The objective of this study was to determine the prevalence of Shiga toxin-producing E. coli (STEC) O157:H7 in colon content and on carcasses from pigs slaughtered at provincially licensed abattoirs (PLAs) in Alberta. In 2017, carcass sponge samples and colon content samples were collected from 504 healthy market hogs at thirty-nine PLAs and analyzed for E. coli O157:H7. Carcass samples...
Mon, 06/22/2020 - 22:17
In August 2018, Public Health England (PHE) was made aware of five probable cases of Shiga toxin‐producing Escherichia coli (STEC) O157:H7 among individuals reporting participation in a mud‐based obstacle race. An additional four cases, identified via routine whole‐genome sequencing, were subsequently linked to the same event. Two of the nine cases were due to secondary household transmission....
Fri, 06/19/2020 - 02:02
Sodium hypochlorite has been used in the food industry to reduce microbial contamination in food; however, the use of sodium hypochlorite is insufficient considering the human health hazards and environmental pollution. To overcome this issue, plant-derived antimicrobial agents have been developed. In our previous study, Cudrania tricuspidata leaf extract (0.4%) was used to wash fresh vegetables...
Tue, 06/16/2020 - 16:00
As a common foodborne pathogen, Escherichia coli O157:H7 produces toxins causing serious diseases. However, traditional methods failed in detecting E. coli O157:H7 cells in the viable but non-culturable (VBNC) state, which poses a threat to food safety. This study aimed at investigating the formation, control, and detection of the VBNC state of E. coli O157:H7. Three factors including medium,...
Fri, 06/05/2020 - 03:38
Shiga toxin (Stx)-producing Escherichia coli (STEC) O157:H7 is an enteric pathogen that causes life-threatening disease in humans, with cattle being major natural reservoirs. A group of STEC O157:H7 with a dramatic combination of high virulence potentials and super-shedder bovine origin have been isolated. Here, an STEC O157:H7 isolate, JEONG-1266, was analyzed by comparative genomics, stx...
Fri, 05/29/2020 - 03:51
This study aimed to identify a suitable non-pathogenic surrogate for industrial validation of the irradiation process by high energy electron beam (5 MeV) of dried seeds. Pumpkin seeds and flax seeds were contaminated with a five-strain Salmonella cocktail comprising five different serovars or a two-strain Escherichia coli cocktail comprising pathogenic strains, including E. coli O157:H7....
Fri, 05/29/2020 - 03:38
Escherichia coli O157:H7 is a common harmful foodborne pathogen that can cause severe diseases at low infectious doses. Traditional lateral flow immunoassay (LFIA) for the rapid screening of E. coli O157:H7 in food suffers from low sensitivity due to its dependence on 20- to 40-nm gold nanoparticles (AuNP) with insufficient brightness as labels. To address this issue, we reported for the first...
Thu, 05/28/2020 - 03:49
Bacteriophage therapy is acknowledged as a potential tool to prevent or treat multidrug resistant bacterial infections. In this study, our major focus was on the bacteriolytic activity of phage EcSw (ΦEcSw) against the emergence of the clinically important Escherichia coli Sw1 and E. coli O157:H7. The amount of the antibiotics were changed in a concentration dependent manner and the ΦEcSw...
Tue, 05/19/2020 - 03:49
More efficient sampling and detection methods of pathogens on fresh produce are needed. The purpose of this study was to compare a novel rinse/membrane filtration method (RMFM) to a more traditional sponge rubbing or stomaching method in processing jalapeño peppers and cantaloupe samples for detection of Escherichia coli , Salmonella enterica , and Listeria monocytogenes . For jalapeño peppers...
Tue, 05/19/2020 - 03:40
Soil microbial communities are often not resistant to the impact caused by microbial invasions, both in terms of structure and functionality, but it remains unclear whether these changes persist over time. Here we used 3 strains of Escherichia coli O157:H7 (E. coli O157:H7), a species used for modelling bacterial invasions, to evaluate the resilience of the bacterial communities from four Chinese...
Sat, 05/16/2020 - 03:36
Treatments of wastewater and fresh produce commonly employ chlorine as an antimicrobial. However, there are increasing levels of concerns regarding the safety and antimicrobial efficacy of chlorine treatments. Numerous studies have reported the antimicrobial properties of chlorine dioxide (ClO2) treatment in a variety of applications but information regarding how ClO2 affects bacteria is limited...
Thu, 05/07/2020 - 03:41
PDT is an emerging new antimicrobial technology, which uses a combination of photosensitizers and light. This study, for the first time, demonstrated that ERY (Red No. 3)‐mediated PDT can be an alternative antimicrobial treatment strategy to the conventional treatments used in tomato juice. Abstract The objectives of this study were to evaluate the efficacy of erythrosine B (ERY, Red No. 3)‐...
Fri, 05/01/2020 - 03:38
Aims The objective of the study was to determine levels of Escherichia coli O157:H7 colonization in the gastrointestinal tract (GIT) of naturally‐shedding cattle shedding the pathogen at low‐ or super‐shedder levels. Methods and Results Over two years, feedlot cattle were sampled multiple times for faecal shedding of E. coli O157:H7. Just prior to harvest (1 to 2 days), animals that were super‐...
Thu, 04/30/2020 - 03:43
Some biological activities of dihydroquercetin (DHQ), including antioxidative, antibacterial and anticancer activities, and the oxidative stability of butter oil supplemented with DHQ were evaluated. The DPPH* radical‐scavenging activity was high for DHQ, with an IC50 of 63.83 ± 3.11 μg/ml, compared to that of Trolox, which had an IC50 of 117.02 ± 5.19 μg/ml, over the concentrations studied (10–...
Fri, 04/24/2020 - 03:36
Aims To evaluate tomato epiphyte lactic acid bacteria (LAB) hydrophobicity and auto‐aggregation as an indicator of bacteria adhesion to tomato. Likewise use LAB adhesion and co‐aggregation as mechanisms to antagonize pathogen attachment. Methods and Results Fifty‐four LAB were screened to evaluate their hydrophobic, auto‐ and co‐aggregative properties against Salmonella Typhimurium, Saintpaul,...
Thu, 04/23/2020 - 03:38
Prolonged exposure of foodborne pathogens (Salmonella Enteritidis [SE], Escherichia coli O157:H7 [EC], and Listeria monocytogenes [LM]) to acid, salt, and cold stress alters their antibiotic resistance. However, the effect of acid, salt, and cold stress on bacterial antibiotic resistance depend on both the bacterial species and the specific antibiotic. Abstract Bacteria with antibiotic‐resistant...
Wed, 04/22/2020 - 03:44
This research study was based on ultraviolet (UVC) light and a combination of UVC-ozone treatments that have recently received attention from the beef processing industry as antimicrobial interventions that leave no chemical residues on products. The objectives were to evaluate effectiveness of UVC and UVC in combination with gaseous ozone treatments for inactivation of pathogenic bacteria on...
Sun, 04/19/2020 - 03:35
Cattle are the asymptomatic reservoirs of Escherichia coli O157:H7 (O157) that preferentially colonizes the bovine recto-anal junction (RAJ). Understanding the influence of O157 on the diversity of the RAJ microbiota could give insights into its persistence at the RAJ in cattle. Hence, we compared changes in bovine RAJ and fecal microbiota following O157 challenge under experimental conditions....
Fri, 04/17/2020 - 03:35
Foodborne diseases are a major cause of illness in Canada. One of the main pathogens causing foodborne illnesses and outbreaks in Canada is Escherichia coli O157:H7.In Canada, from 2008 to 2018, 11 outbreaks of E. coli O157:H7 linked to leafy greens were identified, including 7 linked to romaine lettuce (63.6%), 2 linked to iceberg lettuce (18.2%) and 2 linked to other or unspecified types of...
Thu, 04/16/2020 - 03:30
Escherichia coli O157:H7 (E. coli O157:H7) is an important pathogenic Bacterium that threatens human health. A convenient, sensitive and specific method for the E. coli O157:H7 detection is necessary. We developed two pairs of monoclonal antibodies through traditional hybridoma technology, one specifically against E. coli O157 antigen and the other specifically against E. coli H7 antigen. Using...
Wed, 04/08/2020 - 03:32
Author(s): Seoung Hyuk Kim, Wang June Kim, Seok-Seong Kang The inhibitory effect of Lactobacillus brevis DF01 and Pediococcus acidilactici K10, which had been isolated from kimchi, on enteropathogenic bacterial adhesion to intestinal epithelial cells as well as their probiotic potential was studied. After the incubation of Lb. brevis DF01 and P. acidilactici K10 in simulated gastrointestinal...
Tue, 04/07/2020 - 03:53
Fresh‐cut apples were dip‐coated in antimicrobial/antibrowning solutions. The developed solutions had the dual effectiveness of both controlling browning and reducing pathogenic bacterial populations, which maintained the fresh‐like appearance and texture of apple slices for 35 days and reduced populations of Salmonella to undetectable levels (<1 log) without growth throughout the storage at 4...
Thu, 03/19/2020 - 03:33
Escherichia coli O157:H7 and Salmonella enterica are leading causes of foodborne outbreaks linked to fresh produce. Both species can enter the "viable but nonculturable" (VBNC) state that precludes detection using conventional culture-based or molecular methods. In this study, we assessed propidium monoazide-quantitative PCR (PMA-qPCR) assays and novel methods combining PMA and loop-mediated...
Wed, 03/04/2020 - 01:19
Antimicrobial seed treatments recommended by Canadian guidance for sprouted vegetable production (2,000 ppm hypochlorite for 15-20 minutes or 6-10% hydrogen peroxide for 10 minutes at room temperature) are not fully compliant with organic production principles. We investigated the effect of a sequential treatment consisting of a 10 min soak at 50 0 C in water followed by exposure to a 2.0% H 2 O...
Wed, 03/04/2020 - 01:19
Canadian First Nations communities rely on traditional preservation methods such as  the smoking, drying, and canning of fish and game meats, to ensure long-term food security. Unlike commercial food production, there are no recognized official standards for these methods, thus rendering their efficacy in producing microbiologically safe foods relatively unknown. In this study, 81 fresh or...
Wed, 03/04/2020 - 01:18
Escherichia coli O157:H7 (STEC) acid resistance may aid the pathogen’s ability to cross the human gastric barrier and makes it an organism of concern in acidic foods. Our objective was to determine how STEC acid resistance may correlate with survival during vegetable fermentations. Seven E. coli O157:H7 strains were screened to assess acid resistance in simulated stomach-acid at pH 2. The...
Tue, 03/03/2020 - 10:04
Shane, L. E., A. C. S. Porto-Fett, B. A. Shoyer, R. K. Phebus, H. Thippareddi, A. Hallowell, K. Miller, L. Foster-Bey, S. G. Campano, P. J. Taormina, D. L. Glowski, R. B. Tompkin, and J. B. Luchansky. Coarse ground meat was mixed with non-meat ingredients and starter culture (Pediococcus acidilactici) and then inoculated with an 8-strain cocktail of Shiga toxin-producing Escherichia coli (ca. 7.0...
Tue, 03/03/2020 - 10:01
Jung, Y., A. C. S. Porto-Fett, B. A. Shoyer, L. E. Shane, E. Henry, M. Osoria, and J. B. Luchansky. A total of 514 raw pork samples (395 ground or nonintact and 119 intact samples) were purchased at retail stores in Pennsylvania, Delaware, and New Jersey between July and December 2017. All raw pork samples were screened for serogroup O26, O45, O103, O111, O121, O145, or O157:H7 cells of Shiga...
Tue, 03/03/2020 - 09:59
Jackson-Davis, A., M. Daniel, M. Oliver, J. B. Luchansky, A. C. S. Porto-Fett, and L. Kassama. Shiga toxin-producing Escherichia coli (STEC) has been recognized as a human pathogen since 1982. However, the non-O157 STEC strains have been implicated in many human illnesses and seem to cause disease as severe as the potent E. coli O157: H7. Hence, the objective of this study was to evaluate the...
Tue, 03/03/2020 - 09:56
Porto-Fett, A. C. S., A. Jackson-Davis, L. Kassama, M. Daniel, M. Oliver, Y. Jung, and J. B. Luchansky. High pressure processing (HPP) was evaluated to inactivate Shiga toxin-producing Escherichia coli (STEC) in raw meatballs. Ground meat (>90% lean) was inoculated (ca. 7.0 log CFU/g) with a rifampicin-resistant cocktail of eight STEC strains (O26:H11, O45:H2, O103:H2, O104:H4, O111:H-, O121:...
Sun, 03/01/2020 - 03:27
The resistance of Escherichia coli O157:H7 to disinfection is associated with its ability to form biofilms, mainly constituted by glucans produced by glucosyltransferases. Citral and geraniol, terpenes found in the essential oil of Cymbopogon citratus (EO), have proven antibacterial activity against planktonic E. coli; however, no information was found about their efficacy and mode of action...
Wed, 02/26/2020 - 03:27
In this study we compared nine Shiga toxin (Stx)-producing Escherichia coli O157:H7 patient isolates for Stx levels, stx-phage insertion site(s), and pathogenicity in a streptomycin (Str)-treated mouse model. The strains encoded stx2a, stx1a and stx2a, or stx2a and stx2c. All of the strains elaborated 105-106 cytotoxic doses 50% (CD50) into the supernatant after growth in vitro as measured on...
Thu, 02/13/2020 - 03:40
The Ib-M6 peptide has antibacterial activity against non-pathogenic Escherichia coli K-12 strain. The first part of this study determines the antibacterial activity of Ib-M6 against fourteen pathogenic strains of E. coli O157:H7. Susceptibility assay showed that Ib-M6 had values of Minimum Inhibitory Concentration (MIC) lower than streptomycin, used as a reference antibiotic. Moreover, to predict...
Fri, 02/07/2020 - 03:53
Aims The purpose of this study was to isolate Lactobacillus from gastrointestinal tract of healthy post‐weaning piglets and investigate its synergistic antimicrobial and probiotic effects with ZnO nanoparticles (nZnO). Methods and Results Out of the 128 isolates, L. plantarum BLPL03 was selected based on its excellent acid and bile salt tolerance properties. L. plantarum BLPL03 was sensitive to β...
Thu, 02/06/2020 - 03:33
LWT
Author(s): Atefeh Sadat Kashfi, Yousef Ramezan, Mohammad Reza KhaniAbstractThis study purpose was to investigate the effect of radiofrequency low pressure cold plasma (LPCP) process by using dried peppermint (Mentha piperita) on the reduction in microbial load, antioxidant activity, total phenolic content, and color. The microbial load of the sample was investigated by infecting it with 106 CFU/g...
Thu, 02/06/2020 - 03:30
The effects associated with fat content (0% to 40%) of pork batter and the positions (center and surface) on both ohmic heating (OH) and water bath heating (WB) treated samples, were investigated. The OH achieved a more uniform temperature distribution in pork batter as compared to WB‐ and OH‐treated samples showed lower cooking loss values for all samples tested. Besides, the thiobarbituric acid...

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