Listeria monocytogenes

Thu, 01/21/2021 - 02:02
The essential oil (EO) from the roots of Plectranthus barbatus Andr. (Syn. Coleus forskohlii Briq.) was evaluated for quorum sensing (QS) inhibitory activity. P. barbatus EO was screened for inhibition of QS regulated violacein production in Chromobacterium violaceum (ATCC 12472) wild-type strain. At inhibitory (6.25% v/v) and sub-inhibitory concentrations (3.125% v/v) of the EO, dose-dependent...
Wed, 01/20/2021 - 22:13
Dates are a low moisture ready‐to‐eat fruit which are popular both globally and in the United States. The harvesting and post‐harvest handling of dates could result in the contamination of common produce‐associated foodborne pathogens including E. coli O157:H7, Listeria monocytogenes, and Salmonella spp. In this study, the efficacy of antimicrobial washes for the reduction of foodborne pathogen...
Wed, 01/20/2021 - 22:13
The objective of this work was to optimize a suitable high‐pressure (HP) condition to inactivate artificially inoculated Listeria monocytogenes in silver pomfret (Pampus argenteus) fish medium by employing a wide range of pressure levels (225‐550 MPa) and holding times (5‐30 min). The optimized HP conditions (375 MPa/20 min) were subsequently employed in pomfret fish. Glycine and glutamic acid...
Wed, 01/20/2021 - 10:02
Listeria monocytogenes is a facultative Gram-positive intracellular bacterium that is capable of causing serious invasive infections in pregnant women, resulting in abortion, still-birth, and disseminated fetal infection. Previously, a clinical L. monocytogenes isolate, 07PF0776, was identified as having an enhanced ability to target cardiac tissue. This tissue tropism appeared to correlate with...
Wed, 01/20/2021 - 06:08
Author(s): Andréia Miho Morishita Harada, Maristela S. Nascimento
Wed, 01/20/2021 - 04:02
Wine and alcoholic apple cider are commonly back-sweetened with unpasteurized juice to produce fresh, natural, and palatable sweetened alcoholic beverages. Foodborne pathogens may be introduced from unpasteurized juice into alcoholic beverages through this back-sweetening process. Although pathogens generally do not survive under low pH conditions or high alcohol environment, the die-off of these...
Mon, 01/18/2021 - 22:13
Schematic representation of test system for transfer of L. monocytogenes associated with soil particles to spinach leaves in the presence of antimicrobial agents. Abstract Sporadic contamination of produce with Listeria monocytogenes (Lm) is a recurrent problem. Ways for contamination to occur include transfer of the pathogen to produce from processing surfaces or soil particles to which it has...
Mon, 01/18/2021 - 02:02
Spheroids of intestinal cells (Caco-2) were used to evaluate the adhesion/invasion ability of Listeria monocytogenes (pathogen) and Lactobacillus sakei 1 (potential probiotic). Besides, transcriptomic analyses of Caco-2 cells in three dimensional cultures were done, with the aim of revealing possible host-foodborne bacteria interactions. Result of adhesion assay for L. monocytogenes in Caco-2...
Sun, 01/17/2021 - 06:08
Author(s): Maria A. Gkerekou, Konstantina G. Athanaseli, Anastasia E. Kapetanakou, Eleftherios H. Drosinos, Panagiotis N. Skandamis
Thu, 01/14/2021 - 16:00
The aim of this study was to assess the efficacy of four natural antimicrobial compounds (cinnamaldehyde, eugenol, resveratrol and thymoquinone) plus a control chemical disinfectant (sodium hypochlorite) in inhibiting biofilm formation by Listeria monocytogenes CMCC54004 (Lm 54004) at a minimum inhibitory concentration (MIC) and sub-MICs. Crystal violet staining assay and microscopic examination...
Thu, 01/14/2021 - 16:00
Environmental adaptation of Listeria monocytogenes is a complex process involving various mechanisms that can contribute to their survival in the environment, further spreading throughout the food chain and the development of listeriosis. The aim of this study was to analyze whole-genome sequencing data in a set of 270 strains of L. monocytogenes derived from human listeriosis cases and food and...
Tue, 01/12/2021 - 06:18
Author(s): Dipon Sarkar, David A. Ratkowsky, Bing Wang, John P. Bowman, Mark L. Tamplin Paneer is a fresh, soft ready-to-eat cheese that is susceptible to Listeria monocytogenes contamination, exemplified by product recalls in Australia, Canada, and the USA. Previous research demonstrates that L. monocytogenes grows in paneer, however there are no paneer-specific predictive models that quantify...
Tue, 01/12/2021 - 06:15
Author(s): J.R. Lucas, A. Alía, R. Velasco, M.D. Selgas, M.C. Cabeza Various adverse conditions can trigger defensive mechanisms in Listeria monocytogenes that can increase the virulence of surviving cells. The objective of this study was to evaluate the expression of one stress-response (sigB) and three virulence (plcA, hly, and iap) genes in L. monocytogenes exposed to a sub lethal dose of E-...
Sun, 01/10/2021 - 22:09
Background In recent years high‐pressure processing (HPP) of fruit products has steadily increased due to antimicrobial effectiveness and the retention of nutritional and quality attributes compared to conventional thermal technologies. Edible coatings are already being used to enhance the quality of minimally processed fruits. Thus, apple cubes (AC) and alginate‐vanillin coated apple cubes (AVAC...
Fri, 01/08/2021 - 04:02
The foodborne pathogen Listeria monocytogenes lives as a saprophyte in nature and can adhere to and grows on surfaces as diverse as leaves, sediment, and stainless steel. To discern the mechanisms used by L. monocytogenes for attachment and growth on various surfaces, we studied interactions between the pathogen on lettuce and stainless steel. A panel of 24 strains (23 of Listeria monocytogenes...
Thu, 01/07/2021 - 10:05
Bacteriophages can be used as a biocontrol for the foodborne pathogen Listeria monocytogenes. Propagation of phages is a necessary step for their use in experimental studies and biocontrol applications. Here, we present the complete genomes of three Listeria monocytogenes strains commonly used as propagation hosts for Listeria phages.
Thu, 01/07/2021 - 10:05
Listeria monocytogenes serotype 7 lacks glycosidic constituents in wall teichoic acids. Here, we present the complete genome sequence of L. monocytogenes serotype 7 strain FSL R9-0915 and an analysis of genes known to affect L. monocytogenes antigenicity. This strain is used as a control strain in Listeria phage host range analyses.
Thu, 01/07/2021 - 02:02
Active films have gained increasing interest because of their potential to reduce the amount of additives into foods. The aim of this work was to produce and characterize bi-layer films based on gelatin in both layers, in which the nisin and/or “pitanga” leaf hydroethanolic extract was loaded in the second layer. The bi-layer gelatin-based films were characterized regarding humidity and...
Wed, 01/06/2021 - 16:00
The presence of Listeria monocytogenes in Mozzarella di Bufala Campana Protected Designation of Origin cheeses may depend on curd stretching conditions and post contaminations before packaging. To avoid cross-contamination, thermal treatment of water, brines and covering liquid may become necessary. The present study aimed to improve knowledge about L. monocytogenes thermal resistance focusing on...
Wed, 01/06/2021 - 14:04
The purpose of this research was to characterize Listeria monocytogenes from several environmental and clinical sources and assess the efficacy of single and combined physico-chemical treatments in reducing biofilm on lettuce leaves. PCR analysis of L. monocytogenes isolates collected from different clinical (10 strains) and environmental sources (12 strains) was used to look for the presence of...
Wed, 01/06/2021 - 06:09
Author(s): Mario González-Angulo, Vinicio Serment-Moreno, Laura Clemente-García, Carole Tonello, Isabel Jaime, Jordi Rovira Despite the commercial success of high pressure processing (HPP) in the juice industry and the fact that no foodborne outbreaks have been reported so far, some regulatory agencies still require process validation. However, there is a lack of consensus on various aspects...
Wed, 01/06/2021 - 06:08
Author(s): Adrián Alvarez-Molina, José F. Cobo-Díaz, Mercedes López, Miguel Prieto, María de Toro, Avelino Alvarez-Ordóñez The food processing environments of a newly opened meat processing facility were sampled in ten visits carried out during its first 1.5 years of activity and analyzed for the presence of Listeria monocytogenes. A total of 18 L. monocytogenes isolates were obtained from 229...
Tue, 01/05/2021 - 10:03
ABSTRACT Although all isolates of the foodborne pathogen Listeria monocytogenes are considered to be pathogenic, epidemiological evidence indicates that certain serovar 4b lineages are more likely to cause severe invasive (neuromeningeal, maternal-fetal) listeriosis. Recently described as L. monocytogenes "hypervirulent" clones, no distinctive bacterial trait has been identified so far that could...
Mon, 01/04/2021 - 22:13
The effects of different concentrations of defatted by supercritical CO2 chokeberry pomace extracts isolated by consecutive pressurized ethanol and water on the growth of L. monocytogenes, B. thermosphacta, P. putida, lactic acid bacteria (LAB) and aerobic mesophilic bacteria (AMB) were tested. The extracts at >3.3% concentration effectively inhibited tested bacteria in their cultures. Heating...
Mon, 01/04/2021 - 22:13
This present work shows the potential of dimethyl dicarbonate (DMDC) as a promising approach to ensure quality and safety of coconut water in term of microbial inactivation and minimal changes in quality parameters during cold storage (4 °C). Pathogenic strains including Escherichia coli, Staphylococcus aureus, Salmonella Enteritidis and Listeria monocytogenes were selected for evaluation of...
Sun, 01/03/2021 - 06:09
Author(s): Shogo Yamaki, Yuji Kawai, Koji Yamazaki Bacterial cell filamentation is induced in response to sublethal stress in Listeria monocytogenes. The formation of filamentous cells, which have plural nucleoids and are connected in rows, is thought to result in the underestimation of viable cell counting using the agar plating method. Filamentous cells are able to return to their initial...
Sun, 01/03/2021 - 02:02
Lactic acid bacteria can be considered as natural biopreservative and good biotechnological alternative to food safety. In this study, the antilisterial compounds produced by Enterococcus isolates from the Patagonian environment and their effectiveness for the control of Listeria monocytogenes in a food model were studied. Enterococcus isolates whose cell-free supernatant presented activity...
Sat, 01/02/2021 - 06:15
Author(s): J. Ortiz-Solà, I. Abadias, P. Colàs-Medà, M. Anguera, I. Viñas The efficacy of the water-assisted ultraviolet-C light (WUVC) strategy was evaluated as an alternative to chlorine sanitization and compared to ‘conventional’ dry technology (DUVC) for the inactivation of Salmonella enterica, Listeria monocytogenes and murine norovirus (MNV-1) on strawberries. Strawberries were washed in a...
Sat, 01/02/2021 - 06:12
Author(s): Jessie Usaga, Óscar Acosta, John J. Churey, Olga I. Padilla-Zakour, Randy W. Worobo Increasing consumer demand for high-quality foods has driven adoption by the food industry of non-thermal technologies such as high pressure processing (HPP). The technology is employed as a post-packaging treatment step for inactivation of vegetative microorganisms. In order to evaluate HPP...
Wed, 12/30/2020 - 00:00
Avocado Hass (Persea americana Mill) peel extract (APE) has the potential as a natural ingredient to substitute for chemical preservatives. The objectives of this study were to assess the phytochemical composition by high-performance liquid chromatography–quadrupole time-of-flight mass/mass spectrometry (HPLC-qTOF-MS/MS), total phenolic content (TPC), proanthocyanidin (PAC) content, and...
Fri, 12/25/2020 - 06:10
Author(s): Cristina Serra-Castelló, Anna Jofré, Nicoletta Belletti, Margarita Garriga, Sara Bover-Cid Food safety is often based on the application of several preservative (hurdle) factors whose combination must be smartly selected. The aim of the present study was to evaluate the effect of lactate and diacetate on the high pressure processing (HPP) inactivation of three L. monocytogenes strains...
Wed, 12/23/2020 - 16:00
Flagellar motility is considered an important virulence factor in different pathogenic bacteria. In Listeria monocytogenes the transcriptional repressor MogR regulates motility in a temperature-dependent manner, directly repressing flagellar- and chemotaxis genes. The only other bacteria known to carry a mogR homolog are members of the Bacillus cereus group, which includes motile species such as...
Wed, 12/23/2020 - 16:00
PGLYRP1/Tag-7/PGRP-S is one of mammalian peptidoglycan recognition proteins (PGRPs). Here, we demonstrate that human recombinant PGLYRP1/Tag-7/PGRP-S potentiates the response of murine macrophage-like ANA-1 cells and human macrophages to facultative intracellular pathogen Listeria monocytogenes. PGLYRP1/Tag-7/PGRP-S binds to the surface of L. monocytogenes and other bacterial cells but has no...
Wed, 12/23/2020 - 08:02
by Diana Ibañez-Peinado, Maria Ubeda-Manzanaro, Antonio Martínez, Dolores Rodrigo The antimicrobial capability of chitosan from Tenebrio molitor as compared with chitosan from crustacean (Penaeus monodon) on different pathogenic microorganisms of concern in food safety was studied. The antimicrobial effect was tested at pH 5 and pH 6.2 and at two different initial concentrations (103 or 106 CFU/...
Sun, 12/20/2020 - 22:10
Aims To investigate the effects of the lectin from Punica granatum sarcotesta (PgTeL) on growth, viability, cell structure, biofilm formation and chitinase activity of Listeria monocytogenes. In addition, the effect of PgTeL on the adhesion and invasion of human cells (HeLa) was determined. Methods and Results PgTeL showed bacteriostatic and bactericidal effects on the strains Listeria...
Sat, 12/19/2020 - 22:09
Background The replacement of synthetic preservatives with their natural alternatives is a growing demand in the food industry, which has led to the development of novel methods such as encapsulation of plants essential oil with appropriate physicochemical stability, and antibacterial and organoleptic properties. This study aimed to prepare an optimal nanoemulsion of Thymus daenensis L. essential...
Sat, 12/19/2020 - 08:02
M. Nüesch-Inderbinen et al. A nationwide outbreak of human listeriosis in Switzerland was traced to persisting environmental contamination of a cheese dairy with Listeria monocytogenes serotype 4b, sequence type 6, cluster type 7488. Whole-genome sequencing was used to match clinical isolates to a cheese sample and to samples from numerous sites within the production environment.
Sat, 12/19/2020 - 08:02
Listeria monocytogenes is a foodborne pathogen of global relevance that causes outbreaks and sporadic cases of listeriosis, acquired through the consumption of contaminated products, including milk or meat products and ready-to-eat meat products subjected to intensive handling. The objective of the present study was to classify L. monocytogenes isolated from various food-related sources in the...
Fri, 12/18/2020 - 10:02
A reliable and standardized classification of Listeria monocytogenes is important for accurate strain identification during outbreak investigations. Current whole-genome sequencing (WGS)-based approaches for strain characterization are either difficult to standardize, rendering them less suitable for data exchange, or are not freely available. Thus, we developed a portable and open-source tool,...
Fri, 12/18/2020 - 10:02
Listeria monocytogenes is a foodborne pathogen that causes high rates of hospitalization and mortality in people infected. Contamination of fresh, ready to eat produce by this pathogen is especially troubling because of the ability of this bacterium to grow on produce under refrigeration temperatures. In this study, we created a library of over 8,000 plant phyllosphere-associated bacteria and...
Fri, 12/18/2020 - 10:02
Environmental sanitation in food manufacturing plants promotes food safety and product microbial quality. However, the development of experimental models remains a challenge due to the complex nature of commercial cleaning processes, which include spraying water and sanitizer on equipment and structural surfaces within manufacturing space. Although simple in execution, the physical driving forces...
Fri, 12/18/2020 - 06:07
LWT
Author(s): Fan Li, Qinghua Ye, Moutong Chen, Yuting Shang, Jumei Zhang, Yu Ding, Liang Xue, Shi Wu, Juan Wang, Rui Pang, Tao Lei, Haiyan Zeng, Qingping Wu Detection of the foodborne pathogen Listeria monocytogenes serotype 4c, one of the predominant serotypes in aquatic products, is difficult and time-consuming. Our objective was to identify novel target genes for real-time PCR detection of L....
Thu, 12/17/2020 - 06:17
Author(s): Mohit Bansal, Nitin Dhowlaghar, Ramakrishna Nannapaneni, Divya Kode, Sam Chang, Chander S. Sharma, Christopher McDaniel, Aaron Kiess The objective of this study was to determine if the adaptation at planktonic stage to subinhibitory concentrations (SIC) of sodium hypochlorite (NaOCl) could modulate the biofilm forming ability of five Listeria monocytogenes strains V7, Scott A, FSL-N1-...
Thu, 12/17/2020 - 06:14
Author(s): Elżbieta Maćkiw, Dorota Korsak, Joanna Kowalska, Benjamin Felix, Monika Stasiak, Katarzyna Kucharek, Jacek Postupolski The aim of the present study is to investigate the prevalence and genetic diversity of Listeria monocytogenes in various fresh and frozen vegetable products available in Poland. The samples were collected at retail market within the framework of national official...
Thu, 12/17/2020 - 06:14
Author(s): Fan Li, Qinghua Ye, Moutong Chen, Baoqing Zhou, Xinran Xiang, Chufang Wang, Yuting Shang, Jumei Zhang, Rui Pang, Juan Wang, Liang Xue, Shuzhen Cai, Yu Ding, Qingping Wu The abundant information provided by the pan-genome analysis approach reveals the diversity among Listeria monocytogenes serotypes. The objective of this study was to mine novel target genes using pan-genome analysis...
Tue, 12/15/2020 - 16:00
Listeria monocytogenes is an etiologic agent of listeriosis, and has emerged as an important foodborne pathogen worldwide. In this study, the molecular characteristics of 155 L. monocytogenes isolates from seven food groups in Shanghai, the biggest city in China, were identified using whole-genome sequencing (WGS). Most L. monocytogenes isolates (79.3%) were obtained between May and October from...
Mon, 12/14/2020 - 00:00
Native egg albumin (NEA) was isolated from hen eggs and hydrolyzed by pepsin to produce hydrolyzed egg albumin (HEA). HEA was chemically characterized and screened for its antibacterial activity against 10 pathogenic bacteria (6 Gram (+) and 4 Gram (−)). The SDS-PAGE pattern of NEA showed molecular weights of hen egg albumin subunits ranging from 30 to 180 kDa. The highest intensive...
Fri, 12/11/2020 - 22:09
Contaminated fresh produce has increasingly been implicated in foodborne disease outbreaks. As microbiological safety surveillance in South Africa is limited, a total of 545 vegetable samples (spinach, tomato, lettuce, cucumber, and green beans) were purchased from retailers, street traders, trolley vendors and farmers’ markets. Escherichia coli, coliforms and Enterobacteriaceae were enumerated...
Fri, 12/11/2020 - 16:00
Listeria monocytogenes (Lm) is a foodborne zoonotic pathogen that causes listeriosis with a mortality rate of 20–30%. Serovar 4b and 1/2b isolates account for most of listeriosis outbreaks, however, no listeriosis vaccine is available for either prophylactic or therapeutic use. Here, we developed a triple-virulence-genes deletion vaccine strain, and evaluated its safety, immunogenicity, and cross...
Fri, 12/11/2020 - 06:07
Author(s): Rui Liu, Yunlian Zhang, Shujat Ali, Suleiman A. Haruna, Peihuan He, Huanhuan Li, Qin Ouyang, Quansheng Chen In this study, a simple fluorescence aptasensor was developed for rapid and sensitive detection of Listeria monocytogenes in food. The aptasensor was comprised of two parts. Aptamer-functionalized upconversion nanoparticles (UCNPs) recognized and adhered to the L. monocytogenes...

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