Listeria monocytogenes

Sun, 10/18/2020 - 22:14
This study was aimed at predicting the inactivation of Listeria monocytogenes in cooked beef during unsteady heating. A one‐step approach was firstly applied to determine the thermodynamic coefficient of the beef sample. Then inactivation kinetics were estimated by constructing the empirical heat‐transfer model and the log‐linear dynamic inactivation model. Validations showed that the integrated...
Fri, 10/16/2020 - 22:18
Listeria monocytogenes is an opportunistic pathogen with the ability to adapt to different environmental conditions, resulting in safety issues for food producers. Foods contaminated by L. monocytogenes can represent a risk if consumed by susceptible individuals such as elderly, pregnant women and the immunocompromised. The aim of this study was to evaluate the genetic diversity of a collection...
Tue, 10/13/2020 - 08:05
by Caroline Peron-Cane, José-Carlos Fernandez, Julien Leblanc, Laure Wingertsmann, Arnaud Gautier, Nicolas Desprat, Alice Lebreton Real-time imaging of bacterial virulence factor dynamics is hampered by the limited number of fluorescent tools suitable for tagging secreted effectors. Here, we demonstrated that the fluorogenic reporter FAST could be used to tag secreted proteins, and we implemented...
Tue, 10/13/2020 - 02:04
This study aimed to explore the antimicrobial effects of activated lactoferrin (ALF) and rosemary extract (RE) on Escherichia coli O157:H7, Salmonella Enteritidis and Listeria monocytogenes, and to investigate their application potential in the meat storage. Minimum inhibitory concentrations (MICs) of ALF, RE and ALF–RE combinations were determined via microtiter plate assay. MICs of ALF were 1%...
Sun, 10/11/2020 - 06:16
Author(s): Patrick McKernan, Benjamin Cassidy, Alexis Woodward, James Battiste, Douglas Drevets, Roger Harrison This study validates bacterial anionic phospholipid as a putative molecular target in a novel antibiotic treatment against Gram-positive bacterium Listeria monocytogenes and Gram-negative bacterium Escherichia coli. Bacterial anionic phospholipid expression was targeted with its...
Sun, 10/11/2020 - 06:15
Author(s): David Lynch, Colin Hill, Des Field, Máire Begley Natural methods to control food pathogens are required and bacteriocins have received much interest in this regard. The aim of this study was to investigate the ability of the novel bacteriocin capidermicin to inhibit Listeria monocytogenes. Agar-based deferred antagonism assays were carried out with the capidermicin producer against 17...
Sun, 10/11/2020 - 04:02
Persistent contamination of food manufacturing environments by Listeria monocytogenes is an important public health risk because such contamination events defy standard sanitization protocols, for example, the application of quaternary ammonium compounds such as benzalkonium chloride (BC), providing a source for prolonged dissemination of the bacteria in food products. We performed whole-genome...
Sat, 10/10/2020 - 06:33
Author(s): John Jorgensen, Rebecca Bland, Joy Waite-Cusic, Jovana Kovacevic The widespread nature of Listeria monocytogenes and its presence in soil and agricultural environments, make it a challenging pathogen to control in the produce industry. The objective of this study was to assess diversity of Listeria spp. isolates (n = 113) previously recovered from five produce handling and processing...
Sat, 10/10/2020 - 06:27
Author(s): Even Heir, Lars Erik Solberg, Mats Carlehög, Birgitte Moen, Merete Rusås Jensen, Askild Lorentz Holck Fresh Atlantic salmon (Salmo salar) represents a healthy, nutritious food with global distribution and increasing consumption and economic value. Contaminating Listeria monocytogenes in fresh salmon represents a health hazard to consumers, is linked to extensive product recalls and is...
Sat, 10/10/2020 - 06:26
Author(s): Rugna Gianluca, Carra Elena, Bergamini Federica, Franzini Giuliana, Faccini Silvia, Gattuso Antonietta, Morganti Marina, Baldi Deborah, Naldi Simona, Serraino Andrea, Piva Silvia, Merialdi Giuseppe, Giacometti Federica Listeria monocytogenes contamination in raw pork and ready to eat foods is an important food safety concern, also for the increasing detection of antimicrobial-resistant...
Fri, 10/09/2020 - 08:03
Listeria monocytogenes contamination is of great concern in queso fresco (QF), and listeriosis outbreaks linked to consumption of QF continue to happen. Hurdle approaches such as combining antimicrobials provide an alternative to improve QF safety. In this work, the efficacy of antimicrobial combinations of nisin (NIS), lauric arginate (LAE), and ε-polylysine (EPL) to inhibit L. monocytogenes...
Fri, 10/09/2020 - 06:15
Author(s): Joo-Won Kim, Bhupendra Mistry, Han-Seung Shin, Seok-Seong Kang In the food processing environment, Listeria monocytogenes has the ability to persist on the surfaces, resulting in serious safety concerns. This study aimed to determine the anti-biofilm activity of N-Mannich bases of berberine linking piperazine (MBP) against L. monocytogenes. Among the MBPs used in this study, MBP5 and...
Fri, 10/09/2020 - 06:15
Author(s): Yeon Jin Kim, Hwan Hee Yu, Ye Ji Song, Yeong Jin Park, Na-Kyoung Lee, Hyun-Dong Paik Biofilm-forming Listeria monocytogenes can be highly infectious and difficult to eliminate in the food industry. This study evaluated the anti-biofilm effects of the cell-free supernatant (CFS) of Saccharomyces cerevisiae against L. monocytogenes. To investigate the anti-biofilm mechanism, cell surface...
Thu, 10/08/2020 - 22:20
Co‐microencapsulation of lactobacilli cell free and propolis extract inhibited bacterial growth considerably. Abstract Cell free extracts (CFE) obtained from Lactobacillus plantarum FI 8595 and Lactobacillus reuteri ATCC 55730 alone or in combination with propolis ethanolic or water extracts (1%) were microencapsulated with maltodextrin (25%) before the subsequent spray drying process. They were...
Thu, 10/08/2020 - 02:04
Plasma-functionalized liquids (PFL) emerge as an effective sanitizer with great potential to be against a variety of microorganisms but their applications on seafood products are limited. In the current study, the physicochemical properties of plasma-functionalized water (PFW) and plasma-functionalized buffer (PFB), and their antimicrobial activities on grass carp, were investigated under...
Wed, 10/07/2020 - 06:15
Author(s): Hiroaki Baba, Hajime Kanamori, Risako Kakuta, Hiroki Sakurai, Kengo Oshima, Tetsuji Aoyagi, Mitsuo Kaku We reviewed 18 listeriosis cases in Japan and performed molecular analysis of causative Listeria monocytogenes (LM) isolates. Strains genetically related to those from other countries caused various types of listeriosis, including vascular listeriosis in immunocompetent elderly...
Wed, 10/07/2020 - 02:04
There are several reports on the detection of Listeria monocytogenes in Iran, which has been identified as a major public health problem. In addition, due to drug resistance and strong biofilm formation of this pathogen, new approaches such as using probiotics are needed to combat this bacterium. The aim of this study was to investigate the effect of spore-forming probiotics on the expression of...
Tue, 10/06/2020 - 16:00
Nisin is a commonly used bacteriocin for controlling spoilage and pathogenic bacteria in food products. Strains possessing high natural nisin resistance that reduce or increase the potency of this bacteriocin against Listeria monocytogenes have been described. Our study sought to gather more insights into nisin resistance mechanisms in natural L. monocytogenes populations by examining a...
Tue, 10/06/2020 - 10:03
Cyclic-di-AMP (c-di-AMP) is an essential and ubiquitous second messenger among bacteria. C-di-AMP regulates many cellular pathways through direct binding to several molecular targets in bacterial cells. C-di-AMP depletion is well known to destabilize the bacterial cell wall, resulting in increased bacteriolysis and enhanced susceptibility to cell wall targeting antibiotics. Using the human...
Fri, 10/02/2020 - 04:02
Growing consumer desires for clean label, “natural” products drives more meat processors to cure meat products with natural sources of nitrate or nitrite such as celery juice powder (CJP). One particular challenge for these producers is to identify safe cooling rates in CJP-cured products where extended cooling could allow growth of pathogens. USDA FSIS recently added guidelines for stabilization...
Fri, 10/02/2020 - 04:02
Grocery stores handle fresh produce in large quantities daily. According to the Food and Drug Administration Food Code, food is to be stored at least 15 cm above the floor, and all foods shall be protected from any source of contamination or otherwise discarded. It is reported in the literature that dropped produce could be a potential source of microbial contamination. Both consumers and...
Thu, 10/01/2020 - 06:30
Author(s): Araceli Bolívar, Jean Carlos Correia Peres Costa, Guiomar D. Posada-Izquierdo, Sara Bover-Cid, Gonzalo Zurera, Fernando Pérez-Rodríguez In this study, the bioprotective potential of Lactobacillus sakei CTC494 against Listeria monocytogenes CTC1034 was evaluated on vacuum-packed hot-smoked sea bream at 5 °C and dynamic temperatures ranging from 3 to 12 °C. The capacity of three...
Mon, 09/28/2020 - 02:05
L. monocytogenes continues to be a major health issue in Europe, as well as worldwide. Faster methods, not only for detection, but also for sample preparation are of great interest particularly for this slow-growing pathogen. Immunomagnetic separation has been previously reported to be an effective way to concentrate bacteria, and remove inhibitors. In the present study, different commercial...
Sat, 09/26/2020 - 04:02
Listeria monocytogenes (Lm) is one of the leading causes of death due to foodborne illness, affecting the elderly, pregnant women, neonates and the immune compromised. Serologically, Lm can be classified into 13 serotypes, though only four are typically linked with food contamination and illness. Since 2000, a shift in serotypes involved in listeriosis outbreaks was observed, suggesting that...
Fri, 09/25/2020 - 22:16
SUMMARY Microbiological contamination of chicken meat depends on the conditions under which the animals are reared, slaughtered and processed. The aim of this study was to determine the influence of farm origin and processing stages at slaughterhouse on the microbial safety and quality of chicken. Samples of chicken carcasses from three different farms were taken from a slaughterhouse. Mesophiles...
Fri, 09/25/2020 - 16:00
Macrophages participate to the first line of defense against infectious agents. Microbial pathogens evolved sophisticated mechanisms to escape macrophage killing. Here, we review recent discoveries and emerging concepts on bacterial molecular strategies to subvert macrophage immune responses. We focus on the expanding number of fascinating subversive tools developed by Listeria monocytogenes,...
Fri, 09/25/2020 - 08:02
The emergence of antimicrobial resistance in the food chain and the consumer's demand for safe food without chemical preservatives have generated much interest in natural antimicrobials. Thus, our main goal was to study the mode of action of the crude extract, the enterocins, and the organic acid produced by a bacteriocinogenic Enterococcus faecium strain S6 previously isolated from raw camel...
Thu, 09/24/2020 - 06:20
LWT
Author(s): Gökhan Kürşad İncili, Müzeyyen Akgöl, Mehmet Emin Aydemir, Selçuk Alan, Muhsin Mutlu, Osman İrfan İlhak, Gülsüm Öksüztepe In recent years, the increase in consumer demands for the products that are processed with natural additives has highlighted the use of natural substances in food processing and safety. For this purpose, the aim of this study is to determine the effect of homemade...
Wed, 09/23/2020 - 08:03
Intracellular pathogens are responsible for an enormous amount of worldwide morbidity and mortality, and each has evolved specialized strategies to establish and maintain their replicative niche. Listeria monocytogenes is a facultative intracellular pathogen that secretes a pore-forming cytolysin called listeriolysin O (LLO), which disrupts the phagosomal membrane and, thereby, allows...
Tue, 09/22/2020 - 08:04
Listeria monocytogenes is an important foodborne pathogen that is a serious threat to public health security, and new strategies to control this bacterium in food are needed. HolGH15, derived from Staphylococcus aureus phage GH15, has shown antibacterial activity against several bacterial species. In this work, the antilisterial behavior and effectiveness of HolGH15 are further studied. To...
Fri, 09/18/2020 - 06:12
LWT
Author(s): Chenghui Zhang, Changzhu Li, Mohamed A. Abdel-Samie, Haiying Cui, Lin Lin Listeria monocytogenes (L. monocytogenes) can adhere to biological or non-biological surfaces, including food processing equipment and storage containers. Subsequently, they can contaminate food by forming a biofilm, thereby causing serious food safety problems. In this project, the natural antibacterial agent...
Thu, 09/17/2020 - 10:04
ABSTRACT Listeria monocytogenes is thought to colonize the brain using one of three mechanisms: direct invasion of the blood-brain barrier, transportation across the barrier by infected monocytes, and axonal migration to the brain stem. The first two pathways seem to occur following unrestricted bacterial growth in the blood and thus have been linked to immunocompromise. In contrast, cell-to-cell...
Thu, 09/17/2020 - 10:04
This publication reports the availability of draft genome sequences of 171 Listeria monocytogenes strains isolated from various food-related sources from California between 2007 and 2017. All isolates contain at least two antimicrobial resistance genes.
Sun, 09/13/2020 - 06:12
LWT
Author(s): Haiying Cui, Hong Li, Changzhu Li, Mohamed A. Abdel-Samie, Lin Lin As a natural antibacterial agent, moringa oil (MO, containing palmitic acid, stearic acid and oleic acid as the dominant components) performs promising potential to ensure microbiological safety in food industry. Nevertheless, the antibacterial mechanism of MO is still unclear. In this study, we explored the...
Sat, 09/12/2020 - 04:02
Bacterial foodborne diseases are one of the most important public health issues worldwide but in Ecuador reports on the microbiological quality of foods are scarce. In this cross-sectional study, a total of 450 samples of high-demand Ecuadorian food, including bolon, encebollado, sauces, ceviche, fruits, fruit juices, fruit salads, cheese, raw chicken and ground beef were collected from popular...
Sat, 09/12/2020 - 04:02
A comprehensive understanding of foodborne pathogen diversity in pre-harvest environments is necessary to effectively track pathogens on farms and identify sources of produce contamination. As such, this study aimed to characterize Listeria diversity in wildlife feces and agricultural water collected from a New York State produce farm over a growing season. Water samples were collected from a...
Fri, 09/11/2020 - 16:00
We compared the consistency, accuracy and reproducibility of next-generation short read sequencing between ten laboratories involved in food safety (research institutes, state laboratories, universities and companies) from Germany and Austria. Participants were asked to sequence six DNA samples of three bacterial species (Campylobacter jejuni, Listeria monocytogenes and Salmonella enterica) in...
Fri, 09/11/2020 - 06:15
Author(s): Xu Zhang, Li Ling, Zhiyong Li, Jufang Wang Worldwide, Listeria monocytogenes is a common foodborne pathogen and serotyping is necessary for traceability and surveillance. Due to high accuracy and speed, PCR is commonly used for serotyping by targeting specific genes, such as lmo1118 and ORF2110 for 1/2c-3c and 4b-4d-4e, respectively. Single nucleotide polymorphisms (SNPs) are single...
Sun, 09/06/2020 - 22:17
Aim To study the impact of incorporating micro‐nano‐bubbles (MNBs) in commonly used food antimicrobials (AMs) against Escherichia coli O157:H7 (EC) and Listeria monocytogenes (LM). Methods and Results Air, carbon dioxide (CO2) and nitrogen (N2) were used to incorporate MNBs in city water. Nine‐milliliter AM solutions (with or without MNBs) were individually taken into sterile test‐tubes and mixed...
Sun, 09/06/2020 - 06:16
Author(s): Belén Giménez, Natalia Graiver, Leda Giannuzzi, Noemí Zaritzky The treatment of beef with gaseous ozone (GO) was analyzed and the physicochemical characteristics (texture, lipid oxidation, surface color) and food safety (microbial growth of heterotrophic microflora and inoculated Listeria monocytogenes) were evaluated.
Sun, 09/06/2020 - 06:13
Author(s): Wannes Van Beeck, Cédric Verschueren, Sander Wuyts, Marianne F.L. van den Broek, Mieke Uyttendaele, Sarah Lebeer Artisanal vegetable fermentations are regaining popularity in industrialized countries, but they could be prone to contamination with foodborne pathogens. By simulating home or small-scale restaurant fermentations, we evaluated the microbiological safety of spontaneous...
Wed, 09/02/2020 - 06:11
LWT
Author(s): Ilkin Yucel Sengun, Gulden Kilic, Berna Ozturk In this study, the effects of marination liquids (MLs) prepared using koruk juice (25% and 50%) and dried koruk pomace (1% and 2%) with or without additives [salt (1%) and thyme (0.1%)] on meat safety were determined. In the first stage, the minimum inhibition concentration (MIC) values of MLs were observed in the range of 6.50% - >50...
Sun, 08/30/2020 - 06:14
Author(s): Salvatore Antoci, Luigi Iannetti, Gabriella Centorotola, Vicdalia Aniela Acciari, Francesco Pomilio, Paolo Daminelli, Costanza Romanelli, Anna Beatrice Ciorba, Nicola Santini, Marina Torresi, Anna Ruolo, Filippo Castoldi, Marco Pierantoni, Pietro Noè, Giacomo Migliorati Trade of food of animal origin between countries is carried out according to specific bilateral agreements based on...
Sun, 08/30/2020 - 06:12
Author(s): Saša Vatić, Nemanja Mirković, Jelica R. Milošević, Branko Jovčić, Natalija Đ. Polović Numerous applications of proteolytic enzymes include dissociation of fermented meat products for the enumeration of `foodborne pathogenic bacteria. The use of trypsin for this cause is abandoned due to the high concentration of the enzyme affecting released bacteria. Papain, as a suggested replacement...
Sat, 08/29/2020 - 06:17
LWT
Author(s): Jiacheng Zhou, Eirini Velliou, Seok Hoon Hong The FDA-approved antimicrobial peptide nisin has been used to prevent contamination of food products from Gram-positive pathogens including Listeria monocytogenes (L. monocytogenes). However, some L. monocytogenes strains are tolerant or resistant to nisin treatment at a dose that would normally be lethal. Therefore, alternative or combined...
Fri, 08/28/2020 - 06:15
Author(s): Adam R. Quinn, Ruo Fen Liao, Frost M. Steele, Laura K. Jefferies, Bradley J. Taylor Pathogens in low water activity foods are an important emerging food safety concern due to notable survival and thermotolerance. Limited but growing data are publicly available to compare the thermotolerance of Listeria monocytogenes, Salmonella spp., and Enterococcus faecium NRRL B-2354 (a Salmonella...
Fri, 08/28/2020 - 04:02
The ability of the enterocin-A-B-P-producing Enterococcus faecium KE82 adjunct strain to inactivate Listeria monocytogenes during Galotyri PDO cheese processing was evaluated. Three artisan cheese trials from traditionally ‘boiled’ (85oC) ewe’s milk were processed. The milk cooled at 42oC was divided in two parts: A1 was inoculated with Streptococcus thermophilus ST1 and Lactococcus lactis subsp...
Wed, 08/26/2020 - 10:04
ABSTRACT The facultative intracellular pathogen Listeria monocytogenes, like many related Firmicutes, uses the nucleotide second messenger cyclic di-AMP (c-di-AMP) to adapt to changes in nutrient availability, osmotic stress, and the presence of cell wall-acting antibiotics. In rich medium, c-di-AMP is essential; however, mutations in cbpB, the gene encoding c-di-AMP binding protein B, suppress...
Tue, 08/25/2020 - 22:22
A method based on vapor‐phase advanced oxidation process (AOP) for decontaminating red or green grapes was validated for inactivating Listeria monocytogenes and spoilage molds. A Central Composite Design (CCD) and Response Surface Methodology (RSM) were applied to determine the contribution of UV‐C (254 nm) dose, hydrogen peroxide, and ozone concentration on the lethality toward Aspergillus niger...

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