Listeria monocytogenes

Fri, 10/11/2019 - 03:41
Bacteriophages that infect the foodborne pathogen Listeria monocytogenes were previously isolated from New York dairy farms. The complete genome sequences for three of these Listeria phages, with genome sizes of 64.6 to 65.7 kb, are presented here. Listeria phages LP-010, LP-013, and LP-031-2 are siphoviruses that belong to the genus Homburgvirus.
Fri, 10/11/2019 - 03:36
LWT
Author(s): Yuanyuan Pan, Yan Zhang, Jun-Hu Cheng, Da-Wen SunAbstractEffects of ultrasound pretreatments and dielectric barrier discharge (DBD) cold plasma on the inactivation of Listeria monocytogenes (L. monocytogenes) under various growth temperatures were investigated, and membrane fatty acid profiles of L. monocytogenes were also determined. Fatty acids anteiso-C15:0, anteiso-C17:0 and C15:0...
Wed, 10/09/2019 - 03:29
A laboratory-based study testing 9 Listeria innocua strains independently and a cocktail of 11 Listeria monocytogenes strains was carried out. The aim was to identify suitable L. innocua strain(s) to model L. monocytogenes in inactivation experiments. Three separate inactivation procedures and a hurdle combination of the three were employed: thermal inactivation (55°C), UV-C irradiation (245 nm...
Wed, 10/09/2019 - 03:29
During infection, bacterial pathogens successfully sense, respond and adapt to a myriad of harsh environments presented by the mammalian host. This exquisite level of adaptation requires a robust modulation of their physiological and metabolic features. Additionally, virulence determinants, which include host invasion, colonization and survival despite the host's immune responses and...
Tue, 10/08/2019 - 03:39
by Eric T. Sumrall, Yang Shen, Anja P. Keller, Jeanine Rismondo, Maria Pavlou, Marcel R. Eugster, Samy Boulos, Olivier Disson, Pierre Thouvenot, Samuel Kilcher, Bernd Wollscheid, Didier Cabanes, Marc Lecuit, Angelika Gründling, Martin J. Loessner The intracellular pathogen Listeria monocytogenes is distinguished by its ability to invade and replicate within mammalian cells. Remarkably, of the 15...
Tue, 10/08/2019 - 03:36
Author(s): Beatrix Stessl, Kati Szakmary-Brändle, Ulrike Vorberg, Dagmar Schoder, Martin WagnerAbstractDue to a higher probability for violation of hygiene measures, reconstruction work is a substantial food safety challenge for food business operators (FBOs). Here, we monitored a Listeria monocytogenes contamination scenario during a timely enduring reconstruction period that aimed at an...
Tue, 10/08/2019 - 03:36
Author(s): Yangtai Liu, Wanxia Sun, Tianmei Sun, Leon G.M. Gorris, Xiang Wang, Baolin Liu, Qingli DongAbstractMeat products are commonly regarded as one of the main sources of human listeriosis caused by Listeria monocytogenes. The objective of this study was to estimate the prevalence of L. monocytogenes in a range of meat products from 24 different Chinese regions by using meta-analysis of...
Tue, 10/08/2019 - 03:32
The current study investigates the effect of Lactobacillus reuteri and Lactobacillus plantarum combined with water extract of garlic on microbial growth, chemical changes, and sensory attributes in ground beef samples at refrigeration condition (+4°C) up to 12 days of storage. in vitro study revealed that garlic extract combined with L. reuteri or L. plantarum caused 2.13 and 2.57 log reduction...
Sun, 10/06/2019 - 03:36
LWT
Author(s): Gökhan Kürşad İncili, Pınar Karatepe, Osman İrfan İlhakAbstractThe aim of this study was to determine the single and combined effect of chitosan and Pediococcus acidilactici (B-LC-20) on Escherichia coli O157:H7, Salmonella Typhimurium and Listeria monocytogenes in meatballs. After spiking the samples with pathogens, meatball mixture was divided into 4 groups as control, chitosan, B-LC...
Sat, 10/05/2019 - 03:36
Author(s): Hossein Haghighi, Serge Kameni Leugoue, Frank Pfeifer, Heinz Wilhelm Siesler, Fabio Licciardello, Patrizia Fava, Andrea PulvirentiAbstractThe main aim of this study was to characterize microstructural, physical, optical, mechanical, water barrier and antimicrobial properties of chitosan-polyvinyl alcohol blend films (CS-PVA) enriched with ethyl lauroyl arginate (LAE) (1–10% w/v) for...
Fri, 10/04/2019 - 03:36
LWT
Author(s): Veronica Rodriguez-Martinez, Gonzalo Velázquez, Rosario de Jesús Rodríguez Altaif, Fabian Fagotti, Jorge Welti-Chanes, J. Antonio TorresAbstractFollowing deterministic and probabilistic predictive microbiology procedures, cumulative 48 h exponential growth estimates were obtained for Listeria monocytogenes (Lm) and Pseudomonas putida (Pp) as preservation effectiveness indicators for...
Fri, 10/04/2019 - 03:32
Listeria monocytogenes, in fresh and ready‐to‐eat produce such as whole fresh apples, is of concern as there is no “kill step” in their packing process that would eliminate the pathogenic bacteria. Recent listeriosis outbreaks revealed that insufficient cleaning and sanitation practices in fresh apple packing houses may lead to contamination of fruit with L. monocytogenes. This article discusses...
Thu, 10/03/2019 - 03:30
Dairy manure, a by-product in the dairy industry, is also a potential source of nutrients for crops. However, improper application of biological soil amendments of animal origin can be a source of contamination with enteric foodborne pathogens. A 2-year field study was conducted to evaluate impacts of dairy manure fertilizer application on the microbial safety of red raspberry (Rubus idaeus L)...
Wed, 10/02/2019 - 03:41
In Listeria monocytogenes, it has been proposed that the VirSR two-component signal transduction systems (TCSs) and two ATP-binding cassette (ABC) transporters, VirAB and AnrAB, constitute a complex TCS/ABC transporter system which has been recognized as a unique resistance mode. The role of the putative VirAB-VirSR-AnrAB system in antimicrobial resistance and the respective contributions of the...
Wed, 10/02/2019 - 03:41
Contamination of food during processing is recognized as a main transmission route of Listeria monocytogenes. To prevent microbial contamination, biocides are widely applied as disinfectants in food processing plants. However, there are concerns about the development of antimicrobial resistance in foodborne pathogens due to widespread biocide usage. In our study, 93 L. monocytogenes isolates from...
Wed, 10/02/2019 - 03:35
In Turkey, legislation reduced the salt content in fresh cheeses from 10% to 6.5% in dry matter (DM). This study investigated the effect of salt reduction on the fate of acid‐ and salt‐adapted Escherichia coli O157:H7 and Listeria monocytogenes, as well as the textural quality of recombined nonfat cheese. The cheese was manufactured using milk protein concentrate (85% protein) and skim milk...
Wed, 10/02/2019 - 03:34
The current study evaluated the immunomodulatory effects of Carica papaya pulp and seeds methanol (MeOH) extracts on mice infected with Listeria monocytogenes. Gas chromatography–mass spectrometry analysis identified 10 active constituents in C. papaya seed MeOH extract and 10 compounds in C. papaya pulp MeOH extract. The experimental animals were divided into negative control (G1) group,...
Wed, 10/02/2019 - 03:30
Listeria monocytogenes is an intracellular facultative pathogen that causes listeriosis, a foodborne zoonotic infection. There are differences in the pathogenic potential of L. monocytogenes subtypes and strains. Comparison of the genome sequences among L. monocytogenes pathogenic strains EGD-e and F2365 with nonpathogenic L. innocua CLIP1182 and L. monocytogenes strain HCC23 revealed a set of...
Tue, 10/01/2019 - 03:38
Author(s): Ibukunoluwa Fola Olawuyi, Soo Rin Kim, Dongyup Hahn, Won Young LeeAbstractThis study obtained two novel polysaccharides from okra by enzyme-assisted and combined enzyme-ultrasonic methods while comparing with previously established hot-water, ultrasonic methods, for their food application potentials. Structural characteristics and physicochemical properties were evaluated and their...
Tue, 10/01/2019 - 03:30
Listeria monocytogenes (L. monocytogenes) is a ubiquitous organism that can easily enter the food chain. Infection with L. monocytogenes can cause invasive listeriosis. Since 2014, in Austria, L. monocytogenes isolates from human and food/food-associated samples have been provided on a mandatory basis by food producers and laboratories to the National Reference Laboratory. Since 2017, isolates...
Mon, 09/30/2019 - 17:03
LWT
Author(s): Alonzo A. Gabriel, Katrina Moira D. Melo, Juan Carlos D. MichelenaAbstractThis study was conducted to determine the efficacy of UV-C irradiation for safety and quality of dried bay leaves. Cocktails of microorganisms of the same species were prepared and inoculated onto the leaves, which were thereafter subjected to UV-C irradiation. Results showed that all tested organisms, which...
Mon, 09/30/2019 - 16:54
ABSTRACT Rod-shaped bacteria have two modes of peptidoglycan synthesis: lateral synthesis and synthesis at the cell division site. These two processes are controlled by two macromolecular protein complexes, the elongasome and divisome. Recently, it has been shown that the Bacillus subtilis RodA protein, which forms part of the elongasome, has peptidoglycan glycosyltransferase activity. The cell...
Sun, 09/29/2019 - 03:30
Author(s): Sulaiman F. Aljasir, Catherine Gensler, Lang Sun, Dennis J. D'AmicoAbstractCheeses made from unpasteurized milk are preferred by many consumers for their complex flavors and aromas among other attributes. However, the potential presence and survival of foodborne pathogens remain a concern. There is, therefore, a need for developing practical and science-based preventive strategies to...
Sun, 09/29/2019 - 03:25
Four cases of listeriosis in a hospital (A) in New Zealand were identified in 2012. Pulsed‐field gel electrophoresis used at the time identified four pulsotypes amongst the clinical isolates. Two of the pulsotypes matched to L. monocytogenes isolates obtained from ready‐to‐eat (RTE) meat samples from a RTE producer tested during a nationwide microbiological survey the month prior. The outbreak...
Sat, 09/28/2019 - 03:23
Aims To study the antimicrobial agents of the Bacillus velezensis strain HC6 and assess the application potential of Bacillus velezensis HC6 in maize. Methods and Results We applied a dual culture technique to test the antimicrobial activity of B. velezensis HC6 against bacteria and fungi of common contaminated crops. B. velezensis HC6 showed antagonistic action on pathogenic fungi, including...
Thu, 09/26/2019 - 03:28
LWT
Author(s): Chantelle Human, Dalene de Beer, Marique Aucamp, Isa J. Marx, Christiaan J. Malherbe, Marinda Viljoen-Bloom, Marieta van der Rijst, Elizabeth JoubertAbstractGreen rooibos extract containing high levels of aspalathin, a dihydrochalcone antioxidant, and the carrier chitosan, both an antioxidant and antimicrobial agent, was used to produce a dual-purpose food ingredient powder by spray-...
Thu, 09/26/2019 - 03:22
Considering the growing consumption of artisanal foods worldwide, we aimed to evaluate the microbial safety of Serro artisanal cheese (SAC), produced in Minas Gerais State, Brazil. This cheese is produced with raw milk using 1 of 2 natural starter cultures: “pingo” and “rala.” A total of 53 SAC samples (pingo = 8; rala = 45) were obtained from different farmers and subjected to conventional and...
Sun, 09/22/2019 - 03:31
The foodborne pathogen Listeria monocytogenes can modulate its transcriptome and proteome to ensure its survival during transmission through vastly differing environmental conditions. While L. monocytogenes utilizes a large array of regulators to achieve survival and growth in different intra- and extrahost environments, the alternative sigma factor B and the transcriptional activator of...
Sun, 09/22/2019 - 03:30
Author(s): Sophie Tongyu Wu, John Burnett, Jingjin Wang, Susan R. Hammons, Deklin R. Veenhuizen, Haley F. OliverAbstractWe designed and implemented a comprehensive survey of facilities, management practices, and cleaning and sanitizing frequencies in 30 United States grocery retail produce departments to evaluate Listeria monocytogenes control strategies. Produce department managers completed the...
Sun, 09/22/2019 - 03:28
LWT
Author(s): A. García, L.M. Pérez, G.N. Piccirilli, R.A. VerdiniAbstractSoy sauce is a traditional seasoning for foods available at the global market that is consumed all over the world. This sauce has a unique flavor and also antimicrobial and antioxidant properties. There is a wide range of soy sauces and their differences are mainly based on the manufacturing methods. Variation in chemical...
Sun, 09/22/2019 - 03:25
Significance and Impact of the Study: Treated wastewater effluent contains Listeria monocytogenes which survives conventional wastewater treatment processes and can re‐enter human food chain, thus it is imperative to detect L.  monocytogenes using a rapid and an inexpensive method. To the best of our knowledge, this is the first report of a loop‐mediated isothermal amplification (LAMP) assay,...
Sat, 09/21/2019 - 03:36
The gut microbiome is critical in providing resistance against colonization by exogenous microorganisms. The mechanisms via which the gut microbiota provide colonization resistance (CR) have not been fully elucidated, but they include secretion of antimicrobial products, nutrient competition, support of gut barrier integrity, and bacteriophage deployment. However, bacterial enteric infections are...
Sat, 09/21/2019 - 03:34
Author(s): Hilary M. Tobin, Saurabh R. Lele, Catherine N. Cutter, Ramaswamy C. Anantheswaran, Luke F. LaBordeAbstractIndustrial food slicing equipment that is difficult to clean and sanitize can become a reservoir of Listeria monocytogenes. Some commercial mushroom slicing operations have attempted to manage cross-contamination risks by periodically immersing detachable slicer heads in heated...
Sat, 09/21/2019 - 03:33
Author(s): Marie-Christine Etty, Sabato D'Auria, Carole Fraschini, Stephane Salmieri, Monique LacroixAbstractThis paper presents the effects of the composition of different media (i.e., Tryptic soy broth (TSB), Brain heart infusion (BHI), Listeria enrichment broth (LEB), Fraser broth (FB) and University of Vermont medium (UVM)) on the detection of a short peptide fragment PepD specific to the p60...
Sat, 09/21/2019 - 03:29
In this study, the antibacterial properties of Pimpinella anisum essential oil (PAO) were evaluated against six food‐related bacteria. Afterward, the effects of different concentrations of PAO on microbial, chemical, and organoleptic properties of minced beef were evaluated during refrigerated storage. Based on the findings, PAO was effective against all of the tested strains, where minimum...
Mon, 09/16/2019 - 00:00
Abstract Spices are primarily used as flavor enhancers and have attracted attention as natural food preservatives since their antimicrobial effects were determined. In the present study, the antimicrobial effects, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values on 5 important food-borne pathogenic bacteria were investigated in 20...
Sun, 09/15/2019 - 01:03
Available online 14 September 2019 Author(s): Elpiniki Vandera, Maria Parapouli, Athanasia Kakouri, Anna-Irini Koukkou, Efstathios Hatziloukas, John SamelisABSTRACTThe presence of eight common structural enterocin genes, singly or in varying combinations, in the genome of 15 antagonistic Enterococcus spp. previously isolated from artisan Greek Graviera and Galotyri retail cheeses was tested and...
Sat, 09/14/2019 - 01:03
Available online 12 September 2019 Author(s): Selin Kalkan, Mustafa Remzi Otağ, Mehmet Soner EnginAbstractMethylcellulose (MC) polymer was used to prepare the edible films. Then, the Rheum ribes L. ethanol extract (RE) was added to the films in order to act as a natural antimicrobial agent. All the methylcellulose films were characterized for bioactivity and the physicochemical analysis were...
Wed, 09/11/2019 - 12:54
Abstract Aims The aim of this study was to develop a rapid detection and differentiation method for pathogenic Listeria species in stone fruits. Methods and Results We utilized activated charcoal enrichment media (ACM) to induce overexpression and hypersecretion of pathogenic Listeria virulence proteins which can subsequently be detected via immunoblot analysis. Plum and nectarine slices...
Wed, 09/11/2019 - 01:05
LWT
Publication date: Available online 8 September 2019Source: LWTAuthor(s): Jin-Hee Kim, Sun Jung, Se-Wook OhAbstractMicrobial enrichment of bacteria is essential for detection because generally foodborne pathogens exist on food at very low concentrations. This study described a rapid and simple pretreatment method, combination of bacteria concentration and DNA concentration for detection E. coli...
Wed, 09/11/2019 - 01:05
Available online 9 September 2019 Author(s): Jiaming Huang, Bowen Chen, Huihui Li, Qiao-Hui Zeng, Jing Jing Wang, Haiquan Liu, Yingjie Pan, Yong ZhaoAbstractPhotodynamic inactivation (PDI) is a promising and effective non-thermal sterilization technology. This study aimed to construct the enhanced antibacterial and anti-biofilm blue light-emitting diode PDI with edible photosensitizer curcumin...

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