Listeria monocytogenes

Sat, 02/15/2020 - 03:36
Author(s): María Jesús Gracia, Regina Lázaro, Consuelo Pérez-Arquillué, Rafael Pagán, Sergio Ramos, João Luis Garcia, Susana BayarriAbstractRaw and dry-cured meats have been identified as a potential source of Toxoplasma gondii infection for humans. The present study evaluated the efficacy of an alternative non-thermal food-processing treatment, high hydrostatic pressure, on the viability of T....
Sat, 02/15/2020 - 03:25
Invited review: Advances in nisin use for preservation of dairy products. By Ibarra-Sánchez et al., page 2041. Dairy products, in particular nonfermented products, are prone to contamination with foodborne pathogens, such as Listeria monocytogenes, Staphylococcus aureus, and Salmonella spp. Nisin, a widely used antimicrobial, is effective in certain dairy products. In this review, we discuss the...
Wed, 02/12/2020 - 10:50
Author(s): Aidin Shafipour Yordshahi, Mehran Moradi, Hossein Tajik, Rahim MolaeiAbstractLyophilized postbiotics of Lactobacillus plantarum was prepared and impregnated in bacterial nanocellulose (BNC) by ex-situ method to develop an antimicrobial ground meat wrapping nanopaper. The postbiotics incorporated BNC (P-BNC) films were optimized by response surface methodology and their antimicrobial...
Wed, 02/12/2020 - 03:27
Study objective was to determine efficacy of a bacteriophage suspension against Listeria spp. when applied to three common types of materials used in food manufacturing facilities. Materials included two food contact materials (stainless steel and polyurethane thermoplastic belting) and one noncontact material (epoxy flooring). Coupons of each material were inoculated with a cocktail containing L...
Tue, 02/11/2020 - 03:39
by Chidozie Declan Iwu, Anthony Ifeanyi Okoh Listeria monocytogenes (L. monocytogenes) is a foodborne pathogen and the etiologic agent of listeriosis, which can be disseminated within the agricultural environment particularly soil and irrigation water, contaminate farm produce and cause high mortality and morbidity among vulnerable individuals. This study assessed the incidence and antibiogram of...
Sat, 02/08/2020 - 03:35
Author(s): Rafaela de Melo Tavares, Danilo Augusto Lopes da Silva, Anderson Carlos Camargo, Ricardo Seiti Yamatogi, Luís Augusto NeroAbstractListeria monocytogenes harbor different virulence factors, with a highly heterogeneous distribution between distinct lineages and serotypes. The Listeria Pathogenicity Island 3 (LIPI-3), mainly described in lineage I, encodes for Listeriolysin S (LLS), a...
Fri, 02/07/2020 - 03:53
Aims The purpose of this study was to isolate Lactobacillus from gastrointestinal tract of healthy post‐weaning piglets and investigate its synergistic antimicrobial and probiotic effects with ZnO nanoparticles (nZnO). Methods and Results Out of the 128 isolates, L. plantarum BLPL03 was selected based on its excellent acid and bile salt tolerance properties. L. plantarum BLPL03 was sensitive to β...
Fri, 02/07/2020 - 03:53
Aims Exposure of Listeria monocytogenes to osmotic stress can induce increased resistance to subsequent lethal exposure to cell envelope stressors, such as nisin and bile salts. We wanted to determine if similar cross‐protection phenotypes could occur when L. monocytogenes strains were treated with osmotic stress and exposed to sub‐lethal levels of the cell envelope stressor, bile. Method and...
Thu, 02/06/2020 - 03:37
Author(s): KaWang Li, Xiaoli Etienne, Yu-Chun Chiu, Lisa Jones, Hanna Khouryieh, Wentao Jiang, Cangliang ShenAbstractThis study aims to i) evaluate two triple-wash procedures with an H2O2-peroxyacetic-acid mixer (SaniDate-5.0) to improve microbial safety and quality of butternut squashes and ii) determine the feasibility of the triple-wash application in a processing plant. In study I, fresh...
Thu, 02/06/2020 - 03:27
The current study made an attempt to develop a novel surface plasmon resonance (SPR) sensor using wheat germ agglutinin as a biomolecule for the rapid detection of Listeria monocytogenes. A selective biomolecular interaction of wheat germ agglutinin (WGA) lectin with surface carbohydrate components of L. monocytogenes was used as a biorecognition principle. From the previous studies based on...
Thu, 02/06/2020 - 03:26
Agricultural water is an important source of foodborne pathogens on produce farms. Managing water-associated risks does not lend itself to one-size-fits-all approaches due to the heterogeneous nature of freshwater environments. To improve our ability to develop location-specific risk management practices, a study was conducted in two produce-growing regions to (i) characterize the relationship...
Thu, 02/06/2020 - 03:26
Listeria monocytogenes is an important foodborne pathogenic bacterium that is explicitly threatening public health and food safety. Rapid, simple, and sensitive detection methods for this pathogen are of urgent need for the increasing on-site testing demands. Application of the isothermal recombinase polymerase amplification (RPA) and the lateral flow strip (LFS) in the detection is promising for...
Thu, 02/06/2020 - 03:26
Intestinal microbiota exerts protective effects against the infection of various bacterial pathogens, including Listeria monocytogenes, a major foodborne pathogen whose infection can lead to a disease (listeriosis) with a high fatality rate. As a strategy to mitigate the action of the intestinal microbiota, pathogens often produce antimicrobial proteinaceous compounds such as bacteriocins. In...
Wed, 02/05/2020 - 03:36
Author(s): Wanxia Sun, Tianmei Sun, Xiang Wang, Qing Liu, Qingli DongAbstractA quantitative probabilistic model was developed to estimate the concentration of Listeria monocytogenes in cooked meat products based on presence/absence data and an assumed zero-inflated distribution, i.e. zero-inflated Poisson (ZIP) or zero-inflated Poisson lognormal (ZIPL) distribution. The performance of these two...
Wed, 02/05/2020 - 03:27
The objective of this study was to determine whether seeds of Brassica oleracea var. italica (i.e. broccoli, an edible plant) produce defensins that inhibit phytopathogenic fungi and pathogenic bacteria of clinical significance. Crude extracts obtained from broccoli seeds were fractioned by molecular exclusion techniques and analyzed by liquid chromatography-high-resolution mass spectrometry. Two...
Wed, 02/05/2020 - 03:26
Fresh foods are vulnerable to foodborne pathogens which cause foodborne illness and endanger people’s life and safety. The rapid detection of foodborne pathogens is crucial for food safety surveillance. An in situ-synthesized gene chip for the detection of foodborne pathogens on fresh-cut fruits and vegetables was developed. The target genes were identified and screened by comparing the specific...
Wed, 02/05/2020 - 03:26
Loranthus europaeus is a well-known and important medicinal plant, with a long history of traditional medicine use. Several studies showed that it contains many bioactive compounds with a wide range of pharmacological effects. In light of these past researches, L. europaeus were chosen to consider its potential antimicrobial action. To this aim, different protocols were performed to selectively...
Mon, 02/03/2020 - 03:33
Author(s): Alonzo A. Gabriel, Cecile Leah T. Bayaga, Eiric A. Magallanes, Richard Paolo M. Aba, Karen May N. TanguiligAbstractThis study was conducted to address the dearth in works that simultaneously compare the growth and inactivation behaviors of selected pathogens in different milk products. In worst-case scenarios where hygienic practices are absent and heavy microbiological contaminations...
Sun, 02/02/2020 - 03:35
Author(s): Duleeka P. Kuruwita, Xiuping Jiang, Duncan Darby, Julia L. Sharp, Angela M. FraserAbstractAn estimated 179 million cases of acute gastroenteritis (AGE) occur each year in the United States. Escherichia coli O157:H7 and Listeria monocytogenes, common etiologic agents of AGE, both have low-infectious doses and high environmental resistance. Surfaces contaminated with either pathogen,...
Sat, 02/01/2020 - 04:00
Author(s): El-Hajjaji Soundous, Gérard Amaury, De Laubier Juliette, Di Tanna Sybille, Lainé Aurélie, Patz Viviane, Sindic MarianneAbstractButter is a complex matrix characterized by a high fat content. Existing publications on the behavior of Listeria monocytogenes in this type of food reported contrasted results. This study was performed to provide further information and data about raw milk...
Sat, 02/01/2020 - 04:00
Author(s): Laura Settier-Ramírez, Gracia López-Carballo, Rafael Gavara, Pilar Hernández-MuñozAbstractPVOH-based polymer matrices in the form of films were evaluated as carriers of living Lactococcus lactis subsp. Lactis. These lactic acid bacteria are capable of producing nisin, which is an effective antilisterial peptide. A low percentage (1:0.125 w/w) of yeast extract, gelatin, sodium caseinate...
Fri, 01/31/2020 - 03:29
Salmonella enterica, Listeria monocytogenes, Shigella flexneri, Escherichia coli O157:H7, Vibrio parahaemolyticus, Staphylococcus aureus, Vibrio cholerae, Clostridium botulinum type A, Bacillus cereus, Clostridium perfringens Alpha toxin, and Yersinia enterocolitica are 11 common foodborne pathogens. Traditional bacterial culture methods for detecting pathogens are time‐consuming and labor‐...
Thu, 01/30/2020 - 03:25
General hygienic parameters and selected foodborne pathogens in raw milk cheeses at the retail level were evaluated. A total of 245 raw milk cheese samples were analysed for total bacterial count, Enterobacteriaceae, E. coli, Salmonella spp., Listeria monocytogenes, coagulase-positive Staphylococci, and staphylococcal enterotoxin. Results showed only 3 samples that were not compliant with...
Wed, 01/29/2020 - 04:07
Author(s): Zhen Jia, Weijuan Bai, Xiaoting Li, Ting Fang, Changcheng LiAbstractThis study was aimed at investigating the growth of Listeria monocytogenes (L. monocytogenes) in irradiated and raw salmon. Mathematical models were developed to describe the growth of L. monocytogenes with or without the presence of the native microbiota in salmon. At temperatures between 4 and 35 °C, the growth data...
Wed, 01/29/2020 - 04:01
Author(s): Nguyen Nhat Minh Phuong, Thien Trung Le, John Van Camp, Katleen RaesAbstractMicrobial safety in food products is not always adequately controlled. Chemical antimicrobials which are recognized as hazards to human health are gradually replaced by natural antimicrobial compounds. In the current study, the antimicrobial activity against some Gram-positive and Gram- negative bacteria by the...
Wed, 01/29/2020 - 03:58
LWT
Author(s): Katy A. Bermudez-Beltrán, Juliet K. Marzal-Bolaño, Aura B. Olivera-Martínez, Paula J.P. EspitiaAbstractThis study aimed to prepare a Petit Suisse cheese added with cape gooseberry and supplemented with moringa leaf powder and gelatin. Formulations were: control formulation (F1); a product with 3% gelatin (F2); and product with 3% gelatin and 2% moringa (F3). Microbiological analyses...
Tue, 01/28/2020 - 03:38
Exoelectrogens are able to transfer electrons extracellularly, enabling them to respire on insoluble terminal electron acceptors. Extensively studied exoelectrogens like Geobacter sulfurreducens and Shewanella oneindensis are Gram-negative, but more recently it has been reported that Gram-positive bacteria like Listeria monocytogenes and Enterococcus faecalis also exhibit the ability to transfer...
Tue, 01/28/2020 - 03:38
The physiological relevance of bacterial iron efflux has only recently been appreciated. The Bacillus subtilis P1B4-type ATPase PfeT (peroxide-induced ferrous efflux transporter) was one of the first iron efflux pumps to be characterized, and cells lacking pfeT accumulate high levels of intracellular iron. The pfeT promoter region has binding sites for both PerR, a peroxide sensing Fur-family...
Tue, 01/28/2020 - 03:37
Author(s): David Buckley, Bassam A. Annous, Bryan VinyardAbstractThe diverse food safety issues facing developed countries require innovations to current food safety intervention technologies and chemistries. Several innovations have been made to highly efficacious treatments, such as chlorine dioxide (ClO2). However, many of the innovations stop short of evaluating actual yield, practical...
Tue, 01/28/2020 - 03:29
Industrial aerobic fermentation of natural black olives. Summary The fermentation of natural black olives is currently carried out under anaerobic conditions. This work investigates the aerobic fermentation of this product in tanks containing 16 000 kg of Empeltre olives during two seasons. The microorganisms in brines were yeasts in all cases, whereas lactic acid bacteria growth was only...
Mon, 01/27/2020 - 03:34
Author(s): Tooraj Mehdizadeh, Hossein Tajik, Ali Mojaddar Langroodi, Rahim Molaei, Alireza MahmoudianAbstractThe aim of the current study was to investigate the effects of pomegranate peel extract (PPE) and chitosan-starch (CH-S) composite film incorporated with Thymus kotschyanus essential oil (TEO) on the shelf-life of beef during storage period of 21 days at 4 °C. The physico-mechanical...
Sun, 01/26/2020 - 03:34
Author(s): Leonardo Acuña, Natalia Corbalan, Marcos Quintela-Baluja, Jorge Barros-Velázquez, Augusto BellomioAbstractBacteriocins are antimicrobial peptides synthesised ribosomally. One of the main drawbacks of bacteriocins as food biopreservative agents is their restricted action spectrum. To obtain a broad-spectrum bacteriocin-producing lactic acid bacterium, we engineered a fusion protein...
Sun, 01/26/2020 - 03:32
Author(s): Kyle Ganz, Etsuko Yamamoto, Kate Hardie, Christine Hum, Hussein Hussein, Annie Locas, Marina SteeleAbstractA profile of the microbial safety of cheese in Canada was established based on the analysis of 2955 pasteurized and raw-milk cheeses tested under Canada's National Microbiological Monitoring Program (NMMP) and 2009 raw-milk cheeses tested under the Targeted Survey Program. 97.8%...
Sat, 01/25/2020 - 03:33
Author(s): Ganyu Gu, Samantha Bolten, Joseph Mowery, Yaguang Luo, Connor Gulbronson, Xiangwu NouAbstractChemical sanitizers are commonly used for fresh produce washing to reduce potential presence of foodborne pathogens and spoilage bacteria, and to prevent cross-contamination. In this study, we evaluated the susceptibility of foodborne pathogens, including Shiga-toxigenic Escherichia coli,...
Sat, 01/25/2020 - 03:26
Many pathogenic bacteria use different types of thermosensors to sense increased temperature once inside the host. In this review, we are describing the identification of protein, DNA and RNA thermosensors that took place around 20 years ago. In particular, we describe the events leading to the discovery of the prfA thermosensor in Listeria monocytogenes (depicted). Abstract An outstanding...
Sat, 01/25/2020 - 03:26
While exposure to high levels of toxic metals such as cadmium and arsenic is only experienced in a handful of special habitats and ecosystems, Listeria and other Gram‐positive bacteria have evolved an impressively diverse array of genetic tools for acquiring enhanced tolerance to such metals. Here we summarize this fascinating collection of resistance determinants in Listeria monocytogenes and...
Fri, 01/24/2020 - 03:33
Author(s): Gülden Göksen, Maria José Fabra, H. Ibrahim Ekiz, Amparo López-RubioAbstractThe aim of this work was to develop novel active edible electrospun coatings with antimicrobial properties through the encapsulation of essential oils (EOs) from two widely used spices (Laurus nobilis and Rosmarinus officinalis) for inactivating or reducing spoilage and pathogenic microorganisms. Firstly, EOs...
Thu, 01/23/2020 - 03:35
Isoprenoids are an essential and diverse class of molecules, present in all forms of life, that are synthesized from an essential common precursor derived from either the mevalonate pathway or the nonmevalonate pathway. Most bacteria have one pathway or the other, but the Gram-positive, facultative intracellular pathogen Listeria monocytogenes is unusual because it carries all the genes for both...
Thu, 01/23/2020 - 03:35
Listeria monocytogenes is a foodborne bacterium that causes gastroenteritis, meningitis, or abortion. Listeria induces its internalization (entry) into some human cells through interaction of the bacterial surface protein InlB with its host receptor, the Met tyrosine kinase. InlB and Met promote entry through stimulation of localized actin polymerization and exocytosis. How actin cytoskeletal...
Thu, 01/23/2020 - 03:25
The aim of this study was the assessment of the effect of time exposure, temperature, distance, and organic contaminants on radiant catalytic ionization (RCI) microbicidal effectiveness. The number of all examined bacteria decreased together with time exposure of RCI. The lowest recovery was obtained, both from the rubber surface (6.36 log CFU × cm–2) and steel (6.04 log CFU × cm–2) in the case...
Thu, 01/23/2020 - 03:24
In situ generation of chlorine dioxide to reduce microbial populations on produce surfaces has been shown to be effective on produce models. This study examined the treatment for decontamination of bacterial pathogens on whole cantaloupes and sprout seeds. Whole cantaloupes, mung beans, and alfalfa seeds were inoculated with Salmonella, Listeria monocytogenes, and Shiga toxin–producing...
Thu, 01/23/2020 - 03:24
Listeria monocytogenes has emerged as a food safety concern for several produce commodities. Although L. monocytogenes contamination can occur throughout the supply chain, contamination from the packinghouse environment represents a particular challenge and has been linked to outbreaks and recalls. This study aimed to investigate the prevalence, persistence, and diversity of L. monocytogenes and...
Thu, 01/23/2020 - 03:24
Cheeses made with unpasteurized milk are a safety concern due to possible contamination with foodborne pathogens. Listeria monocytogenes and Escherichia coli O157:H7 have been implicated in several outbreaks and recalls linked to Gouda cheese made with unpasteurized milk. The U.S. Food and Drug Administration Code of Federal Regulations requires cheeses made with unpasteurized milk to be aged at...
Wed, 01/22/2020 - 03:35
Listeria monocytogenes is a Gram-positive Firmicute that causes food-borne infections, in part due to its ability to use multiple strategies, including biofilm formation, to survive adverse growth conditions. As a potential way to screen for genes required for biofilm formation we harnessed the ability of bacteria to accumulate mutations in the genome over time, diverging the properties of...
Wed, 01/22/2020 - 03:35
Transition metals are essential for life, but are toxic when in excess. Metal ion intoxication may result from the mismetallation of essential metal-dependent enzymes with a non-cognate metal. To begin to identify enzymes and processes that are susceptible to mismetallation, we have selected for strains with increased resistance to Mn(II) and Co(II). In Bacillus subtilis, cells lacking the MntR...
Wed, 01/22/2020 - 03:25
Previous studies on the influence of food matrix fat content on thermal inactivation kinetics of food pathogens have shown contradictory results due to the combined influence of fat content and other factors such as composition. Therefore, thermal inactivation of Listeria monocytogenes at 59, 64, and 69°C was systematically investigated in emulsion and gelled emulsion food model systems with...
Wed, 01/22/2020 - 03:25
The foodborne pathogen Listeria monocytogenes survives exposure to a variety of stresses including desiccation in the food industry. Strand-specific RNA sequencing was applied to analyze changes in the transcriptomes of two strains of L. monocytogenes (Lm 568 and Lm 08-5578) during desiccation [15°C, 43% relative humidity (RH)] on food grade stainless steel surfaces over 48 h to simulate a...
Wed, 01/22/2020 - 03:25
Long non-coding RNAs (lncRNAs) have emerged as key regulators of gene expression in several biological systems. The long intergenic RNA-erythroid pro-survival (lincRNA-EPS) has been shown to play a critical role in restraining inflammatory gene expression. However, the function of lincRNA-EPS during bacterial infections remains unknown. Here, we demonstrate that following infection with the...
Tue, 01/21/2020 - 03:31
Author(s): Virginia Filipello, Lapo Mughini-Gras, Silvia Gallina, Nicoletta Vitale, Alessandro Mannelli, Mirella Pontello, Lucia Decastelli, Marc W. Allard, Eric W. Brown, Sara LomonacoAbstractListeriosis is a foodborne illness characterized by a relatively low morbidity, but a large disease burden due to the severity of clinical manifestations and the high case fatality rate. Increased...
Tue, 01/21/2020 - 03:29
LWT
Author(s): Moussa S. Diarra, Yousef I. Hassan, Glenn S. Block, John C.G. Drover, Pascal Delaquis, B. Dave OomahAbstractCranberry (Vaccinium macrocarpon) fruits are known for their high polyphenolics content making them a rich source of antioxidants. These polyphenolics have been reported to promote human health and are gaining attention for their antimicrobial activities against foodborne...

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