Salmonella

Sun, 07/05/2020 - 06:12
Author(s): Ling Jin, Ting Li, Tan Yang, Xuehua Liang, Bin Wu, Dengchao Zou, Liwen Hu, Ganhui Huang, Jinsheng Zhang The monitoring and control of foodborne pathogenic microorganisms to protect food safety is of great significance. A biosensor based on superparamagnetic ultra-small iron oxide nanoparticles (USIO NPs) combined with membrane filtration and low field nuclear magnetic resonance (LF-NMR...
Sat, 07/04/2020 - 06:15
Author(s): Shuangfang Hu, YigangYu, Ziquan Lv, Jianzhong Shen, Yuebin Ke, Xinglong Xiao Salmonella Enteritidis is a major cause of foodborne gastroenteritis and is thus a persistent threat to global public health. The acid adaptation response helps Salmonella survive exposure to gastric environment during ingestion. In a previous study we highlighted the damage caused to cell membrane and the...
Fri, 07/03/2020 - 16:00
The objective of this study was to explore the genetic and biological features of the tet(M)-harboring plasmid pTS14 in Salmonella enterica strain S14 isolated from a chicken fecal sample. Plasmid pTS14 was identified by conjugation, S1-pulsed-field gel electrophoresis (PFGE), Southern hybridization, and plasmid sequencing. The biological characteristics of pTS14 were assessed via stability,...
Fri, 07/03/2020 - 10:02
The aim of this research was to investigate the efficacy of the duty ratio and applied voltage in the inactivation of pathogens in soybean curd by pulsed ohmic heating (POH). The heating rate of soybean curd increased rapidly as the applied voltage increased, although the duty ratio did not affect the temperature profile. We supported this result by verifying that electrical conductivity...
Fri, 07/03/2020 - 08:02
by Zhao Chen, Dai Kuang, Xuebin Xu, Narjol González-Escalona, David L. Erickson, Eric Brown, Jianghong Meng We sequenced 25 isolates of phenotypically multidrug-resistant Salmonella Indiana (n = 11), Typhimurium (n = 8), and Enteritidis (n = 6) using both MinION long-read [SQK-LSK109 and flow cell (R9.4.1)] and MiSeq short-read (Nextera XT and MiSeq Reagent Kit v2) sequencing technologies to...
Thu, 07/02/2020 - 08:02
by Csaba Varga, Patience John, Martin Cooke, Shannon E. Majowicz Nontyphoidal Salmonella enterica (NTS) causes a substantial health burden to human populations in Canada and worldwide. Exposure sources and demographic factors vary by location and can therefore have a major impact on salmonellosis clustering. We evaluated major NTS serotypes: S. Enteritidis (n = 620), S. Typhimurium (n = 150), S....
Thu, 07/02/2020 - 06:16
Author(s): Xiao Fei, Qiuchun Li, John Elmerdahl Olsen, Xinan Jiao S. Pullorum and S. Enteritidis are closely related in genetic terms, but they show very different pathogenicity and host range. S. Enteritidis infects many different hosts, usually causing acute gastroenteritis, while S. Pullorum is restricted to avian, where it causes systemic disease in young animals. The reason why they differ...
Thu, 07/02/2020 - 06:13
Author(s): John A. Porter, Amit Morey, Emefa A. Monu Essential oils in combination with other antimicrobials can be added to food products to reduce the levels of target microbes below the infectious dose required to cause human illness. The purpose of this study was to investigate the antimicrobial efficacy of white mustard essential oil (WMEO) and carvacrol against Salmonella in ground chicken...
Thu, 07/02/2020 - 04:02
Pet food has been identified as a source of pathogenic bacteria, including Salmonella and Escherichia coli. A recent outbreak linked to Salmonella -contaminated pet treats infected over 150 people in the United States. The mechanism by which contaminated pet food leads to human illness has not been explicated. Pet owners’ food safety knowledge and their pet food handling practices have not been...
Thu, 07/02/2020 - 02:02
Food poisoning from consumption of food contaminated with non-typhoidal Salmonella spp. is a global problem. A modified high resolution DNA melting curve analysis (m-HRMa) was introduced to provide effective discrimination among closely related HRM curves of amplicons generated from selected Salmonella genome sequences enabled Salmonella spp. to be classified into discrete clusters. Combination...
Wed, 07/01/2020 - 22:16
Eudragit RS100 nanoparticles containing Baccharis dracunculifolia DC essential oil (N‐EO) was developed by the nanoprecipitation method. N‐EO presented an average diameter of 151.6 nm, zeta potential (ζ) of +51.7 mV, and encapsulation efficiency of 99.4%. The antibacterial activity of N‐EO and free essential oil (EO) was evaluated in BHI broth against Staphylococcus aureus, Bacillus cereus,...
Wed, 07/01/2020 - 06:10
LWT
Author(s): Marianna M. Furtado, Beatriz S. Silva, César Faviero, Verônica O. Alvarenga, Anderson S. Sant’Ana In this study, dry inocula were developed using four different carriers [talc, fine sand (0.05–0.2 mm), regular sand (0.2–0.6 mm), and calcium carbonate] for validation of inactivation steps of low moisture foods against Salmonella enterica. Besides, the changes in aw and the survival of S...
Wed, 07/01/2020 - 00:00
Antimicrobial resistance is one of today’s major public health challenges. Infections caused by multidrug-resistant bacteria have been responsible for an increasing number of deaths in recent decades. These resistant bacteria are also a concern in the food chain, as bacteria can resist common biocides used in the food industry and reach consumers. As a consequence, the search for...
Tue, 06/30/2020 - 16:00
One complication of malaria is increased susceptibility to invasive bacterial infections. Plasmodium infections impair host immunity to non-Typhoid Salmonella (NTS) through heme-oxygenase I (HO-I)-induced release of immature granulocytes and myeloid cell-derived IL-10. Yet, it is not known if these mechanisms are specific to NTS. We show here, that Plasmodium yoelii 17XNL (Py) infected mice had...
Tue, 06/30/2020 - 10:03
ABSTRACT Nontyphoidal salmonellae (NTS) are exposed to reactive oxygen species (ROS) during their residency in the gut. To survive oxidative stress encountered during infection, salmonellae employ several mechanisms. One of these mechanisms involves the multidrug efflux pump MacAB, although the natural substrate of this pump has not been identified. MacAB homologs in pseudomonads secrete products...
Tue, 06/30/2020 - 10:03
ABSTRACT Efflux is an important mechanism in Gram-negative bacteria conferring multidrug resistance. Inhibition of efflux is an encouraging strategy to restore the antibacterial activity of antibiotics. Chlorpromazine and amitriptyline have been shown to behave as efflux inhibitors. However, their mode of action is poorly understood. Exposure of Salmonella enterica serovar Typhimurium and...
Tue, 06/30/2020 - 10:03
ABSTRACT Many bacteria use flagellum-driven motility to swarm or move collectively over a surface terrain. Bacterial adaptations for swarming can include cell elongation, hyperflagellation, recruitment of special stator proteins, and surfactant secretion, among others. We recently demonstrated another swarming adaptation in Escherichia coli, wherein the chemotaxis pathway is remodeled to decrease...
Tue, 06/30/2020 - 10:02
Biofilms exist in complex environments including the intestinal tract as a part of the gastrointestinal microbiota. The interaction of planktonic bacteria with biofilms can be influenced by material properties of the biofilm. During previous confocal studies, we observed amyloid curli-containing Salmonella Typhimurium and Escherichia coli biofilms appeared rigid. In these studies, Enterococcus...
Tue, 06/30/2020 - 02:04
Hydrogen sulfide (H2S) detection is a screening method for distinguishing and identifying Salmonella strains from other bacteria in the intestine. Incidences of H2S-negative Salmonella have recently been reported in different countries. Although a high resistance rate against antimicrobial agents has been reported for H2S-positive Salmonella in many regions of the world, there is increasing...
Tue, 06/30/2020 - 00:00
The continuous collection and analysis of updated data on the antimicrobic resistance among bacterial strains represent the essential core for the surveillance of this problem. The present work aimed to investigate the occurrence of antimicrobial resistance among Salmonella serovars isolated in foods in 2015–2019. A total of 178 Salmonella strains belonging to 39 serovars were tested...
Sun, 06/28/2020 - 06:10
Author(s): Ghazaleh Salimi, Zeinab E. Mousavi, Hossein Kiani Salmonella contamination has been always regarded as one of the main issues in different food products especially fresh meat, while its detection time is normally beyond the shelf life of fresh products. In this paper, a 16S RNA based FISH method was developed for the detection of Salmonella in minced lamb meat. The parameters affecting...
Sun, 06/28/2020 - 06:10
Author(s): Ki-Hoon Lee, Ji-Young Lee, Pantu Kumar Roy, Furkanur Rahaman Mizan, Iqbal Hossain, Si Hong Park, Sang-Do Ha Salmonella is one of the main foodborne pathogens that affect humans and farm animals. The Salmonella genus comprises a group of food-transmitted pathogens which cause highly prevalent foodborne diseases throughout the world. The aim of this study was to appraise the viability of...
Sun, 06/28/2020 - 06:10
Author(s): Tomi Obe, Rama Nannapaneni, Wes Schilling, Li Zhang, Chris McDaniel, Aaron Kiess Salmonella is a poultry-borne pathogen that causes illness throughout the world. Consequently, it is critical to control Salmonella during the process of converting broilers to poultry meat. Sanitization of a poultry processing facility, including processing equipment, is a crucial control measure that is...
Sat, 06/27/2020 - 06:15
Author(s): Angela Parry-Hanson Kunadu, Richard Yaw Otwey, Lydia Mosi Fresh chicken meat is retailed mostly in informal live bird markets (LBM), cottage farms and registered supermarkets. LBM are street food operations that slaughter, dress and trade live or dressed poultry on demand. Here, we provide data on microbiological hazards from LBM, supermarkets and cottage farms in Accra, and evidence...
Sat, 06/27/2020 - 06:14
Author(s): Anna Townsend, Shaoting Li, David A. Mann, Xiangyu Deng Quasimetagenomics refers to the sequencing of a modified food microbiome to facilitate combined detection and subtyping of targeted pathogens in a single workflow. Through quasimetagenomic sequencing, pathogens are detected and subtyped in a shortened time frame compared to traditional culture enrichment and whole genome...
Fri, 06/26/2020 - 22:16
Rat‐associated zoonoses transmitted through faeces or urine are of particular concern for public health because environmental exposure in homes and businesses may be frequent and undetected. To identify times and locations with greater public health risks from rats, we investigated whether rat characteristics, environmental features, socioeconomic factors, or season could predict rat infection...
Fri, 06/26/2020 - 06:11
Author(s): Line Nielsen, Susanne Knøchel Raw egg-based dishes present a safety risk when eggs of uncertain Salmonella status are used. Here, the inactivation of four Salmonella serovars at different combinations of acid, pH and temperature were investigated. Strains of egg or broiler-associated Salmonella serovars Enteritidis, Infantis, Typhimurium, and a heat resistant Senftenberg 775W were...
Thu, 06/25/2020 - 10:02
Recovery from enteric bacterial illness often includes a phase of organismal shedding over a period of days to months. The monitoring of this process through laboratory testing forms the foundation of public health action to prevent further transmission. Regulations in most jurisdictions in the United States exclude individuals who continue to shed certain organisms from sensitive occupations and...
Wed, 06/24/2020 - 10:02
Two nonamidated host defense peptides named Pin2[G] and FA1 were evaluated against three types of pathogenic bacteria: two (Staphylococcus aureus UPD13 and Pseudomonas aeruginosa UPD3) isolated from diabetic foot ulcer patients, and another (Salmonella enterica serovar Typhimurium [ATCC 14028]) from a commercial collection. In vitro experiments showed that the antimicrobial performance of the...
Wed, 06/24/2020 - 10:02
A total of 2,283 Salmonella isolates were recovered from 18,334 samples, including samples from patients with diarrhea, food of animal origin, and pets, across 5 provinces of China. The highest prevalence of Salmonella spp. was detected in chicken meats (39.3%, 486/1,237). Fifteen serogroups and 66 serovars were identified, with Salmonella enterica serovars Typhimurium and Enteritidis being the...
Wed, 06/24/2020 - 08:02
Phosphorothioate (PT) DNA modifications—in which a nonbonding phosphate oxygen is replaced with sulfur—represent a widespread, horizontally transferred epigenetic system in prokaryotes and have a highly unusual property of occupying only a small fraction of available consensus sequences in a genome. Using Salmonella enterica as a model, we asked a question...
Wed, 06/24/2020 - 06:11
Author(s): Jin Hwa Park, Mi Seon Kang, Kyung Min Park, Hee Young Lee, Gyeong Sik Ok, Min Seon Koo, Seok In Hong, Hyun Jung Kim The present study aimed to develop and validate a predictive model of Salmonella spp. and Staphylococcus aureus growth in fresh egg under isothermal and non-isothermal (fluctuating temperature) conditions and also estimate infection probability based on Monte Carlo...
Wed, 06/24/2020 - 04:03
We aimed to perform serotyping and the antimicrobial resistance profile of Salmonella spp. and Listeria monocytogenes strains isolated from raw meats imported illegally into Brazil along the borders of Argentina and Uruguay. Distinct isolates of Salmonella spp. (n=6) and L. monocytogenes (n=25) obtained from 270 of these food products of earlier work were serotyped and tested for antimicrobial...
Tue, 06/23/2020 - 22:14
The objective of this study was to evaluate the possible synergistic interactions on antibacterial and antioxidant efficacy of two plant essential oils (EOs) Eucalyptus caesia Benth and Dracocephalum multicaule Montbr & Auch in vitro and in pear slices. Antibacterial combination effect was evaluated against seven important pathogenic bacteria (Bacillus cereus, Listeria monocytogenes,...
Tue, 06/23/2020 - 16:00
On-site detection demands are quickly increasing to control foodborne pathogenic bacteria along with the long food supply chains. Combining the isothermal recombinase polymerase amplification (RPA) with lateral flow strips (LFSs) is a promising molecular detection approach for the short reaction time, low isothermal condition, and simple and “instrument-free” procedure. However, the method comes...
Tue, 06/23/2020 - 10:02
Salmonella Typhimurium uses a type three secretion system (T3SS) encoded on the Salmonella pathogenicity island 1 (SPI1) to invade intestinal epithelial cells and induce inflammatory diarrhea. The SPI1 T3SS is regulated by numerous environmental and physiological signals, integrated to either activate or repress invasion. Transcription of hilA, encoding the transcriptional activator of the SPI1...
Tue, 06/23/2020 - 10:02
Recent studies have determined that inflammasome signaling plays an important role in driving intestinal epithelial cell (IEC) responses to bacterial infections, such as Salmonella enterica serovar Typhimurium. There are two primary inflammasome pathways, canonical (involving caspase-1) and noncanonical (involving caspase-4 and -5 in humans and caspase-11 in mice). Prior studies identified the...
Mon, 06/22/2020 - 06:11
Author(s): Akshatha Kotian, Vankadari Aditya, Kadeeja Jazeela, Iddya Karunasagar, Indrani Karunasagar, Vijaya Kumar Deekshit Bacterial cells adopt various strategies to adapt themselves in diverse environmental conditions. Salmonella is one such bacteria with diverse mechanisms to survive, replicate and infect in wide host range. This study aims at investigating the biofilm-forming ability of...
Mon, 06/22/2020 - 06:10
Author(s): Kapudeep Karmakar, Sindhu Krishna, Sabyasachi Majumdar, Utpal Nath, Karaba N. Nataraj, N.B. Prakash, Dipshikha Chakravortty Soil-borne Salmonella is associated with a large number of food-related disease outbreaks linked to pre-harvest contamination of plants (like tomato) in agricultural fields. Controlling the spread of Salmonella at field is very important in order to prevent...
Sun, 06/21/2020 - 22:11
BACKGROUND The development of brewer's spent grain protein films with potential active packaging properties was investigated. Films were prepared by casting protein dispersions at different pHs (2, 8, 11), plasticizers (polyethylene glycol –PEG– or glycerol), and levels (0–0.25 g g−1) of PEG. Mechanical, water‐barrier and solubility, optical, antioxidant (reducing power, ABTS•+ and lipidic...
Sun, 06/21/2020 - 02:02
Photothermal effect of nanomaterial has been applied to the establishment of rapid detection method such as test strip. Previously, the detector for the photothermal test strip method mainly was a bulky thermal imaging camera or an infrared-sensing temperature sensor of which the detection data obtained is unstable due to its susceptibility to environmental changes. Herein, a resistive...
Sat, 06/20/2020 - 22:16
Purpose Salmonellosis is one of several reportable diseases in Ontario (ON). Two or more cases of the same serotype that are linked to a common exposure or related to one another in time and/or space are considered a potential outbreak. While laboratory data can help to determine the molecular relatedness of cases, results may take up to several weeks. This study aimed to assess the utility of...
Sat, 06/20/2020 - 06:14
Author(s): Sonia Ktari, Boutheina Ksibi, Kais Ghedira, Laetitia Fabre, Sophie Bertrand, Sonda Maalej, Basma Mnif, Faouzia Rhimi, Simon Le Hello, Adnene Hammami Typhimurium is one of the main Salmonella serovar responsible for non-typhoidal gastro-enteritis in Tunisia. Here, we aimed to assess the genetic diversity of 88 clinical Salmonella Typhimurium strains recovered during 14 years from 2000...
Fri, 06/19/2020 - 04:03
The Florida Complaint and Outbreak Reporting System (FL-CORS) database is used by the Florida Department of Health’s Food and Waterborne Disease Program (FWDP) as one of the tools to detect foodborne disease outbreaks. We present a descriptive and spatial network analysis of the FL-CORS data collected during 2015-2018. We also quantified foodborne disease outbreaks (FBO) which were investigated...
Thu, 06/18/2020 - 10:02
Complete genome sequences of eight isolates of Salmonella enterica subsp. enterica from Canadian wild birds were determined by MinION and Illumina MiSeq sequencing. Assembled chromosomes had an average size of 4,833,662 bp. Salmonella enterica serovar Worthington obtained from partridge and quail carried 267-kb plasmids, which contained multiple antimicrobial resistance genes.
Thu, 06/18/2020 - 10:02
The microbiota plays a vital role in maintaining gut health and influences the overall performance of chickens. Most gut microbiota-related studies have been performed in broilers, which have different microbial communities compared to those of layers. The normal gut microbiota of laying chickens is dominated by Proteobacteria, Firmicutes, Bacteroidetes, Fusobacteria, and Actinobacteria at the...
Thu, 06/18/2020 - 10:02
Food safety is a new area for novel applications of metagenomics analysis, which not only can detect and subtype foodborne pathogens in a single workflow but may also produce additional information with in-depth analysis capabilities. In this study, we applied a quasimetagenomic approach by combining short-term enrichment, immunomagnetic separation (IMS), multiple-displacement amplification (MDA...
Thu, 06/18/2020 - 10:02
Dissemination of extended-spectrum-cephalosporin (ESC)-resistant Salmonella, especially extended-spectrum-β-lactamase (ESBL)-producing Salmonella, is a concern worldwide. Here, we assessed Salmonella carriage by food workers in Japan to clarify the prevalence of ESC-resistant Salmonella harboring blaCTX-M. We then characterized the genetic features, such as transposable elements, of blaCTX-M...
Thu, 06/18/2020 - 10:02
The "Moore swab" is a classic environmental surveillance tool whereby a gauze pad tied with string is suspended in flowing water or wastewater contaminated with human feces and harboring enteric pathogens that pose a human health threat. In contrast to single volume "grab" samples, Moore swabs act as continuous filters to "trap" microorganisms, which are subsequently isolated and confirmed using...
Wed, 06/17/2020 - 16:00
This study identifies a strain of Salmonella enterica subspecies enterica serovar Enteritidis that harbors a highly unusual virulence plasmid. During the characterisation of a group of S. Enteritidis isolates, 10 isolates recovered from Canadian duck production facilities, of which seven were phage type 9b and three were closely related atypical phage types, failed detection by a PCR targeting...

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