Salmonella

Sun, 02/16/2020 - 03:42
Food safety problems pose a great threat to the health of consumers with the greatest burden in developing countries. Street-vended foods play a key role in providing many urban dwellers with cheap, nutritious, and accessible food, but when prepared in an unhygienic and unregulated environment, they could contribute to increased food safety burden. The study investigated the microbiological...
Sat, 02/15/2020 - 03:35
Author(s): Dan Hai, Xingpeng Yin, Zhaoxin Lu, Fengxia Lv, Haizhen Zhao, Xiaomei BieAbstractSalmonella is considered one of the most important foodborne pathogens and is commonly associated with the consumption of eggs, chicken meat, chicken intestines and other animal products. Multidrug resistant (MDR) Salmonella strains are highly adaptive and have been responsible for several foodborne disease...
Sat, 02/15/2020 - 03:32
Author(s): Lucie Collineau, Brennan Chapman, Xu Bao, Branavan Sivapathasundaram, Carolee A. Carson, Aamir Fazil, Richard J. Reid-Smith, Ben A. SmithAbstractSalmonella Heidelberg resistant to ceftiofur (a third-generation cephalosporin antimicrobial agent) in broiler chicken products pose a risk to public health in Canada. The objective of this study was to assess the extent of that risk and to...
Fri, 02/14/2020 - 03:35
Author(s): Samir Subedi, Lihui Du, Amritha Prasad, Barun Yadav, M.S. RoopeshAbstractSeveral low water activity (aw) foods have been recently associated with Salmonella related outbreaks and recalls. The use of high intensity light pulses generated by light-emitting diodes (LEDs) is a novel approach to achieve microbial inactivation. This study compares the ability of light emitted by LEDs at...
Fri, 02/14/2020 - 03:27
Aims Salmonella cells desiccated in an environment with low‐water activity (aw) show longer survival times and enhanced resistance to heat. However, little is known about the cellular ultrastructure of Salmonella in low‐aw environment in relation to the survival and persistence during desiccation. Meterials and Results In this study, Salmonella Enteritidis strain PT30 was dehydrated by exposure...
Thu, 02/13/2020 - 04:06
Intensive poultry production due to public demand raises the risk of contamination, creating potential foodborne hazards to consumers. The prevalence and microbial load of the pathogens Campylobacter, Salmonella, Staphylococcus aureus, and Escherichia coli was determined by standard methods at the farm level. After disinfection, swab samples collected from wall crevices, drinkers, and vents were...
Thu, 02/13/2020 - 03:50
Chicken breast meat is considered as the main source of Salmonella infection in humans. The aim of this study was to isolate lytic bacteriophages specific for Salmonella enterica serovars Enteritidis and examine their efficacy in a cocktail for the biocontrol of Salmonella spp. in raw chicken breast meat. Four lytic phages belonging to the Myoviridae and Siphoviridae families were isolated from a...
Wed, 02/12/2020 - 10:52
Author(s): Yuqiao Jin, Juming Tang, Mei-Jun ZhuAbstractSalmonella is a leading cause of foodborne illness associated with low-moisture foods. In addition to being able to survive in low-moisture environments during long storage, Salmonella has shown sharply increased thermal tolerance making it difficult to control in low-moisture foods. This research utilized soy protein powder as a food career...
Wed, 02/12/2020 - 10:50
Author(s): Shengqi Rao, Guangwei Xu, Xiangning Lu, Ruyi Zhang, Lu Gao, Qingyan Wang, Zhenquan Yang, Xinan JiaoAbstractCarvacrol is an effective natural antimicrobial and antioxidant agent; however, its poor aqueous solubility and high volatility limit its application in food systems. Ovalbumin can encapsulate hydrophobic molecules, thereby improving aqueous solubility and reducing volatility. The...
Wed, 02/12/2020 - 10:50
Author(s): B. Tenderis, B. Kılıç, H. Yalçın, A. ŞimşekAbstractFoodborne illnesses affect the health of consumers worldwide, and thus searching for potential antimicrobial agents against foodborne pathogens is given an increased focus. This research evaluated the influence of sodium lactate (SL), encapsulated (e) and unencapsulated (u) polyphosphates (PP; sodium tripolyphosphate, STP; sodium acid...
Wed, 02/12/2020 - 10:49
Author(s): Nasim Hatamzade Isfahani, Shaban Rahimi, Mohammad Javad Rasaee, Mohammad Amir Karimi Torshizi, Taghi Zahraei Salehi, Jesse L. GrimesAbstractThe antibacterial properties of egg yolk antibodies have been known for many years. Enhanced antibiotic resistance has resulted in increased need for using these antibodies as an alternative. In the present study, generation, capsulation, and...
Wed, 02/12/2020 - 10:49
Author(s): Shaban Rahimi, Sophia Kathariou, Oscar Fletcher, Jesse L. GrimesAbstractSalmonella and Campylobacter are considered major public health burdens worldwide, and poultry are known to be one of the main reservoirs for these zoonotic pathogens. This study was conducted to evaluate the effect of a commercial probiotic or direct-fed microbial (DFM) Calsporin (CSP), and prebiotic or mannan...
Wed, 02/12/2020 - 10:49
Author(s): Graham A.J. Redweik, Zachary R. Stromberg, Angelica Van Goor, Melha MellataAbstractCommercial poultry farms are increasingly threatened by bacterial infections from avian pathogenic Escherichia coli (APEC) and broad-host Salmonella serovars. Recombinant attenuated Salmonella vaccines (RASV) elicit cross-reactive immune responses against APEC in chickens; however, assessment of broad...
Wed, 02/12/2020 - 10:49
Author(s): Muhammad Saeed, Muhammad Naveed, Jakob Leskovec, Asgahr Ali kamboh, Ihsanullah Kakar, Kifayat Ullah, Fawwad Ahmad, Muhammad Sharif, Asif Javaid, Mubasher Rauf, Mohamed E. Abd El-Hack, Mervat A. Abdel-Latif, Sun ChaoAbstractGuduchi (Tinospora cordifolia) is a well-recognized and widely distributed traditional plant that is used successfully in Indian Ayurveda medicine. T. cordifolia has...
Wed, 02/12/2020 - 10:49
Author(s): Michał Patrzałek, Maja Kosecka-Strojek, Klaudia Lisowska-Łysiak, Magdalena Trela, Mariusz Kot, Magdalena Gawlak, Dawid Liszka, Mieczysław Sajewicz, Barbara Tombarkiewicz, Krzysztof Pawlak, Jacek Międzobrodzki, Marcin W. LisAbstractThe spatial network structure of Dergall is based on substances nontoxic to humans and the environment which, when applied on solid surfaces, creates a...
Wed, 02/12/2020 - 03:35
ABSTRACT Flagellin, the agent of prokaryotic flagellar motion, is very widely distributed and is the H antigen of serology. Flagellin molecules have a variable region that confers serotype specificity, encoded by the middle of the gene, and also conserved regions encoded by the two ends of the gene. We collected all available prokaryotic flagellin protein sequences and found the variable region...
Wed, 02/12/2020 - 03:25
Probiotics are important bacteria due to their benefit on human health. In this study, four strains of lactic acid bacteria from chicken bile were isolated and the strain with the best antimicrobial activity was selected for further identification and evaluation on its probiotic traits and safety. The strain was identified as Enterococcus faecium by biochemical characterization and 16S rDNA gene...
Tue, 02/11/2020 - 03:38
Salmonella enterica serovar Typhimurium colonizes and invades host intestinal epithelial cells using the Type Three Secretion System (T3SS) encoded on Salmonella Pathogenicity Island 1 (SPI1). The level of SPI1 T3SS gene expression is controlled by the transcriptional activator HilA, encoded on SPI1. Expression of hilA is positively regulated by three homologous transcriptional regulators, HilD,...
Tue, 02/11/2020 - 03:26
Group A streptococcus (GAS) is a versatile pathogen that causes a wide spectrum of diseases in humans. Invading host cells is a known strategy for GAS to avoid antibiotic killing and immune recognition. However, the underlying mechanisms of GAS resistance to intracellular killing need to be explored. Endothelial HMEC-1 cells were infected with GAS, methicillin-resistant Staphylococcus aureus (...
Tue, 02/11/2020 - 03:25
The aim of the present study was to estimate the prevalence of Salmonella and investigate the dominant serovars distribution in raw beef and to screen the isolated serovars for the prescense of beta-lactamases and virulence genes. A total of 150 samples of raw beef sold at butcher shops (n = 75) and supermarkets (n = 75) in Karachi city were collected (50 samples each from muscles, lymph nodes,...
Sat, 02/08/2020 - 03:36
Author(s): Corrella S DetweilerBacterial pathogens must resist host innate immunity to cause disease. While Gram-negative bacteria have a protective outer membrane, this membrane is subject to host-induced damage that makes these pathogens vulnerable. We developed a high content screening platform that identifies compounds that cause the killing of the bacterial pathogen Salmonella enterica in...
Sat, 02/08/2020 - 03:35
Author(s): Silvia Guillén, María Marcén, Pilar Mañas, Guillermo CebriánAbstractIn this work the resistance of 15 strains belonging to 11 serovars of Salmonella enterica subsp. enterica to several different environmental stresses (acid, hydrogen peroxide, NaCl and heat) and non-thermal food preservation technologies (HHP, PEF, UV) was determined and compared. Results obtained showed that...
Sat, 02/08/2020 - 03:35
Author(s): Siyuan Zhou, Tony Jin, Shiowshuh Sheen, Guohua Zhao, LinShu Liu, Vijay Juneja, Kit YamAbstractChlorine dioxide (ClO2) is an effective disinfectant used in the sanitization of fresh produce. Glucono delta-lactone (GDL), widely used as an acidifier during food processing, can be partially hydrolyzed to become a weak acid—gluconic acid under chemical equilibrium upon dissolution...
Sat, 02/08/2020 - 03:27
Aims The purpose of this study was to isolate Lactobacillus from gastrointestinal tract of healthy post‐weaning piglets and investigate its synergistic antimicrobial and probiotic effects with ZnO nanoparticles (nZnO). Methods and Results Out of the 128 isolates, L. plantarum BLPL03 was selected based on its excellent acid and bile salt tolerance properties. L. plantarum BLPL03 was sensitive to β...
Fri, 02/07/2020 - 04:19
Here, we present the annotated genome of Shemara, a siphophage of Salmonella enterica. The Shemara genome is 44 kb with 83 predicted protein-coding genes. At the nucleotide and amino acid levels, Shemara is most similar to phages in the Guernseyvirinae subfamily.
Fri, 02/07/2020 - 04:10
Author(s): Feng Xu, Chongtao Ge, Hao Luo, Shaoting Li, Martin Wiedmann, Xiangyu Deng, Guangtao Zhang, Abigail Stevenson, Robert C.s Baker, Silin TangAbstractThe use of whole genome sequencing (WGS) data generated by short-read sequencing technologies such as the Illumina sequencing platforms has been shown to provide reliable results for Salmonella serotype prediction. Emerging long-read...
Fri, 02/07/2020 - 04:07
Author(s): Danielle M. Schultze, Ricardo Couto, Feral Temelli, Lynn M. McMullen, Michael GänzleAbstractLow water activity (aW) foods permit the survival of low-infectious dose pathogens including Escherichia coli and Salmonella. Desiccation of non-heat resistant E. coli and Salmonella enterica increases their heat resistance; therefore, alternative methods are necessary to ensure the safety of...
Thu, 02/06/2020 - 03:37
Author(s): KaWang Li, Xiaoli Etienne, Yu-Chun Chiu, Lisa Jones, Hanna Khouryieh, Wentao Jiang, Cangliang ShenAbstractThis study aims to i) evaluate two triple-wash procedures with an H2O2-peroxyacetic-acid mixer (SaniDate-5.0) to improve microbial safety and quality of butternut squashes and ii) determine the feasibility of the triple-wash application in a processing plant. In study I, fresh...
Thu, 02/06/2020 - 03:26
Agricultural water is an important source of foodborne pathogens on produce farms. Managing water-associated risks does not lend itself to one-size-fits-all approaches due to the heterogeneous nature of freshwater environments. To improve our ability to develop location-specific risk management practices, a study was conducted in two produce-growing regions to (i) characterize the relationship...
Wed, 02/05/2020 - 03:38
by Franziska Nienhaus, Diana Meemken, Clara Schoneberg, Maria Hartmann, Thomas Kornhoff, Thomas May, Sabrina Heß, Lothar Kreienbrock, Anna Wendt There are growing demands to ensure animal health and, from a broader perspective, animal welfare, especially for farmed animals. In addition to the newly developed welfare assessment protocols, which provide a harmonised method to measure animal health...
Wed, 02/05/2020 - 03:27
The objective of this study was to determine whether seeds of Brassica oleracea var. italica (i.e. broccoli, an edible plant) produce defensins that inhibit phytopathogenic fungi and pathogenic bacteria of clinical significance. Crude extracts obtained from broccoli seeds were fractioned by molecular exclusion techniques and analyzed by liquid chromatography-high-resolution mass spectrometry. Two...
Wed, 02/05/2020 - 03:26
Fresh foods are vulnerable to foodborne pathogens which cause foodborne illness and endanger people’s life and safety. The rapid detection of foodborne pathogens is crucial for food safety surveillance. An in situ-synthesized gene chip for the detection of foodborne pathogens on fresh-cut fruits and vegetables was developed. The target genes were identified and screened by comparing the specific...
Wed, 02/05/2020 - 03:26
: Bacterial genotoxins (BTGX) induce DNA damage, which results in senescence or apoptosis of the target cells if not properly repaired. Three BTGXs have been identified: the cytolethal distending toxin (CDT) family produced by several Gram-negative bacteria, the typhoid toxin produced by several Salmonella enterica serovars, and colibactin, a peptide-polyketide, produced mainly by the...
Wed, 02/05/2020 - 03:26
Loranthus europaeus is a well-known and important medicinal plant, with a long history of traditional medicine use. Several studies showed that it contains many bioactive compounds with a wide range of pharmacological effects. In light of these past researches, L. europaeus were chosen to consider its potential antimicrobial action. To this aim, different protocols were performed to selectively...
Tue, 02/04/2020 - 04:06
Author(s): Tatiane Milkievicz, Vinicius Badia, Vanessa Barreira Souza, Daniel Angelo Longhi, Alessandro Cazonatto Galvão, Weber da Silva RobazzaAbstractThe objective of this study was to develop and validate a model to predict the growth of Salmonella in chicken meat under different isothermal and dynamic temperature conditions. Three different primary and secondary models and combinations were...
Tue, 02/04/2020 - 03:43
CTX-MSHVplasmid transferconjugationenteric pathogensantibiotic resistance gastroenteritis
Tue, 02/04/2020 - 03:42
The polymyxin antibiotic colistin has been used in decades for treatment and prevention of infectious diseases in livestock. Nowadays, it is even considered as last-line treatment option for severe human infections caused by multidrug- and carbapenem-resistant Gram-negative bacteria. Therefore, the discovery of plasmid-mediated mobile colistin resistance (mcr) genes raised major public health...
Mon, 02/03/2020 - 03:33
Author(s): Alonzo A. Gabriel, Cecile Leah T. Bayaga, Eiric A. Magallanes, Richard Paolo M. Aba, Karen May N. TanguiligAbstractThis study was conducted to address the dearth in works that simultaneously compare the growth and inactivation behaviors of selected pathogens in different milk products. In worst-case scenarios where hygienic practices are absent and heavy microbiological contaminations...
Sun, 02/02/2020 - 03:31
LWT
Author(s): Stefani Machado Lopes, Eduardo César TondoAbstractSpaghetti alla carbonara is a traditional Italian dish, which the sauce made of raw egg yolks is heated using only the heat of cooked pasta. Concerns about the safety of this preparation have been raised due the possibility of egg yolks be contaminated by Salmonella and the heat treatment may not be sufficient for total Salmonella...
Sat, 02/01/2020 - 03:56
Although disinfection procedures are widely implemented in food environments, bacteria can survive and present increased virulence/resistance. Since little is known about these phenomena regarding biofilms, this study aimed to investigate the effect of chemical disinfection on biofilm-derived cells of Salmonella Enteritidis. Using a reference strain (NCTC 13349) and a food isolate (350), biofilm...
Sat, 02/01/2020 - 03:42
Salmonella, a highly virulent food-borne pathogen transmitted through food, can cause severe infectious diseases in a large number of people through a single outbreak, due to its low infective doses. In this study, a flow cytometry (FCM)-based method was developed for the rapid detection of single viable Salmonella cells with dual staining of fluorescein isothiocyanate (FITC)-labeled anti-...
Fri, 01/31/2020 - 03:37
Sixty-six Salmonella enterica serovar Typhimurium isolates carrying incompatibility group FIB (IncFIB) plasmids were sequenced to further characterize the IncFIB plasmid-encoded factors associated with virulence and antimicrobial resistance genes. In addition to the IncFIB plasmid, many of these isolates harbored additional plasmids encoding virulence and antimicrobial resistance genes.
Fri, 01/31/2020 - 03:29
Salmonella enterica, Listeria monocytogenes, Shigella flexneri, Escherichia coli O157:H7, Vibrio parahaemolyticus, Staphylococcus aureus, Vibrio cholerae, Clostridium botulinum type A, Bacillus cereus, Clostridium perfringens Alpha toxin, and Yersinia enterocolitica are 11 common foodborne pathogens. Traditional bacterial culture methods for detecting pathogens are time‐consuming and labor‐...
Fri, 01/31/2020 - 03:28
Seaweed is a source of protein that can help overcome the anticipated challenges of a growing world population and the current challenges for finding alternatives for animal proteins in the Western diet. Thus far, data on the safety of seaweed for feed and food purposes in the Western world are scattered. This study aimed to review the available knowledge on the presence of food safety hazards in...
Thu, 01/30/2020 - 03:37
ABSTRACT Dissemination of antibiotic resistance (AR) genes, often on plasmids, leads to antibiotic-resistant bacterial infections, which is a major problem for animal and public health. Bacterial conjugation is the primary route of AR gene transfer in the mammalian gastrointestinal tract. Significant gaps in knowledge about which gastrointestinal communities and host factors promote plasmid...
Thu, 01/30/2020 - 03:36
Pathogenic microorganisms use various mechanisms to conserve energy in host tissues and environmental reservoirs. One widespread but often overlooked means of energy conservation is through the consumption or production of molecular hydrogen (H2). Here, we comprehensively review the distribution, biochemistry, and physiology of H2 metabolism in pathogens. Over 200 pathogens and pathobionts carry...
Thu, 01/30/2020 - 03:31
LWT
Author(s): Alonzo A. Gabriel, Israel N. NepomucenoAbstractVirgin Coconut Oil (VCO) consumption as functional food has recently become popular together with increasing evidence of its health benefits. However, cold-pressed VCO types are highly manually produced without the application of kill step to ensure safety from possible pathogenic contaminants. The study therefore determined the...
Thu, 01/30/2020 - 03:29
Many laboratories sampling foods for Salmonella are interested only in presence or absence of Salmonella, so only one colony may be selected. The objectives of this study were to use two selective enrichment broths and two selective agar plating media for Salmonella recovery from naturally contaminated broiler carcass rinsates and evaluate these media combinations on Salmonella serotypes...

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