Salmonella

Wed, 10/21/2020 - 10:02
Enteric fever, caused by Salmonella enterica serovar Typhi (S. Typhi) and S. enterica serovar Paratyphi (S. Paratyphi), is a common travel-related illness. Limited data are available on the antimicrobial resistance (AMR) patterns of these serovars among travelers. Records of travelers with a culture-confirmed diagnosis seen during or after travel from January 2007 to December 2018 were obtained...
Wed, 10/21/2020 - 10:02
The spread of antibiotic-resistant bacteria presents a global health challenge. Efficient surveillance of bacteria harboring antibiotic resistance genes (ARGs) is a critical aspect to controlling the spread. Increased access to microbial genomic data from many diverse populations informs this surveillance but only when functional ARGs are identifiable within the data set. Current, homology-based...
Wed, 10/21/2020 - 00:00
Ocellatins are peptides produced in the skins of frogs belonging to the genus Leptodactylus that generally display weak antimicrobial activity against Gram-negative bacteria only. Peptidomic analysis of norepinephrine-stimulated skin secretions from Leptodactylus insularum Barbour 1906 and Leptodactylus nesiotus Heyer 1994, collected in the Icacos Peninsula, Trinidad, led to the purification and...
Tue, 10/20/2020 - 10:03
Upon biofilm formation, production of extracellular matrix components and alteration in physiology and metabolism allows bacteria to build up multicellular communities which can facilitate nutrient acquisition during unfavorable conditions and provide protection toward various forms of environmental stresses to individual cells. Thus, bacterial cells within biofilms become tolerant against...
Sat, 10/17/2020 - 12:01
ABSTRACT This study was designed to evaluate the synergistic effect of phage (P22) and antibiotic on the inhibition of Salmonella Typhimurium exposed to ceftriaxone (CEF) and ciprofloxacin (CIP). The effect of phage and antibiotic treatments was evaluated by plaque size, disk diffusion, antibiotic susceptibility and phage multiplication assays. The sequential treatment effect of phage and...
Fri, 10/16/2020 - 22:16
Aims This study aimed to evaluate the effects of three Bacillus probiotics on Salmonella Typhimurium, and interleukin‐8 (IL‐8) gene expression in the co‐culture of the Bacillus and the pathogen in vitro. Methods and Results Bacillus subtilis, Bacillus indicus, and Bacillus coagulans were initially turned to spore and heat‐inactivated forms. The cellular damages of the probiotics on the HT‐29...
Fri, 10/16/2020 - 22:15
BACKGROUND Animal manure frequently harbors pathogenic microorganisms such as Salmonella spp and diarrheagenic Escherichia coli. A defined microbial consortium such as Effective Microorganisms (EM) can potentially be used as a biocontrol against manure‐borne human pathogens such as Salmonella and pathogenic E. coli. The objective of the study was to investigate the efficacy of EM to decontaminate...
Fri, 10/16/2020 - 10:03
Salmonella enterica serovar Typhimurium is a prevalent incitant of enteritis in human beings and nonhuman animals. It has been proposed that host defense responses incited by Salmonella allow the bacterium to overcome colonization resistance. Piglets (n = 24) were orally inoculated with S. enterica serovar Typhimurium DT104 or buffer alone, and the host and microbial responses were temporally...
Fri, 10/16/2020 - 04:02
Raw pet food, comprised of raw meat and vegetables, has increased in popularity in recent years. Multiple surveys and frequent recalls indicate that this commodity has a high risk of contamination with Salmonella and other foodborne pathogens. Improved screening methods are needed to meet the growing demand for testing. This matrix verification study aimed to apply a Salmonella loop-mediated...
Fri, 10/16/2020 - 04:02
Red meat is associated with Salmonella outbreaks resulting in negative impacts for the processing industry. Little work has been reported on the use of dry heat as opposed to moist heat against Salmonella on red meat. We determined the effect of drying at 25°C and dry heat at 70°C with ~10% relative humidity (RH) for 1 h against eleven Salmonella strains of multiple serovars on beef, lamb, goat,...
Fri, 10/16/2020 - 00:01
The emergence of multidrug resistance (MDR), including colistin resistance, among Enterobacteriaceae recovered from food animals poses a serious public health threat because of the potential transmission of these resistant variants to humans along the food chain. Village chickens or Ayam Kampung are free-range birds and are preferred by a growing number of consumers who consider these chickens to...
Thu, 10/15/2020 - 16:00
The classical bordetellae possess several partially characterized virulence mechanisms that are studied in the context of a complete extracellular life cycle in their mammalian hosts. Yet, classical bordetellae have repeatedly been reported within dendritic cells (DCs) and alveolar macrophages in clinical samples, and in vitro experiments convincingly demonstrate that the bacteria can survive...
Thu, 10/15/2020 - 08:03
The aim of this study was to generate knowledge on the most important milk quality and safety attributes, including somatic cell count (SCC), total bacterial count (TBC), Escherichia coli, Salmonella, and Brucella spp. antibodies and antibiotic residues in milk in the chain from farm to milk collection center (MCC) in Rwanda. In addition, we investigated farm and management factors associated...
Wed, 10/14/2020 - 16:00
When a new activity evolves by changes in a pre-existing enzyme this is likely to reduce the original activity, generating a functional trade-off. The properties of this trade-off will affect the continued evolution of both functions. If the trade-off is strong, gene duplication and subsequent divergence would be favored whereas if the trade-off is weak a bi-functional enzyme could evolve that...
Wed, 10/14/2020 - 16:00
Post-transcriptional regulation mediated by regulatory small RNAs (sRNAs) has risen as a key player in fine-tuning gene expression in response to environmental stimuli. Here, we show that, in Salmonella enterica, the central metabolic regulator CRP-cAMP differentially regulates the sRNAs CsrB and CsrC in a growth phase-dependent manner. While CsrB expression remains unchanged during growth, CsrC...
Wed, 10/14/2020 - 08:03
M. Maes et al. Salmonella enterica serovar Typhi H58, an antimicrobial-resistant lineage, is globally disseminated but has not been reported in Latin America. Genomic analysis revealed 3 independent introductions of Salmonella Typhi H58 with reduced fluoroquinolone susceptibility into Chile. Our findings highlight the utility of enhanced genomic surveillance for typhoid fever in this region.
Wed, 10/14/2020 - 06:13
Author(s): D.V.T. Nair, T.J. Johnson, S.L. Noll, A. Kollanoor Johny A dairy-originated probiotic bacterium, Propionibacterium freudenreichii subsp. freudenreichii B3523 (PF) was found to be effective in reducing multidrug-resistant Salmonella Heidelberg (MDR SH) colonization in turkey poults (2-wk-old) and growing (7-wk-old) and finishing (12-wk-old) turkeys. In this study, we explored the...
Wed, 10/14/2020 - 06:13
Author(s): Bruno S. Mendonça, Willian R. de Oliveira, Rhaquel S. Pereira, Luciana R. Santos, Laura B. Rodrigues, Elci L. Dickel, Luciane Daroit, Fernando Pilotto A new poultry litter disinfection methodology against pathogenic Salmonella spp. serovars using gaseous ammonia is proposed in this study. In laboratory, the poultry litter was disposed into plastic containers and experimentally...
Tue, 10/13/2020 - 22:19
P. hydropiper ethanolic extract: Chlorogenic acid, ferulic acid, rutin, myricetin andquercetin. P. hydropiper essential oil: dodecanal, caryophyllene, caryophyllene oxide, decanal, α‐caryophyllene, citronellol, heptadecanal, linalool and phytol. Ethanolic extract and essential oil of P. hydropiper exhibited antimicrobial activity against three bacteria Staphylococcus aureus 6538P, Escherichia...
Tue, 10/13/2020 - 06:23
Author(s): Lijun Hu, Guojie Cao, Eric W. Brown, Marc W. Allard, Li M. Ma, Ashraf A. Khan, Guodong Zhang Whole genome sequencing (WGS) is a valuable tool in research on foodborne pathogens. In this study, a total of 143 isolates of Salmonella serotypes Enteritidis, Typhimurium, and Heidelberg sourced from eggs and chickens were analyzed for their antimicrobial resistance profiles using WGS data....
Tue, 10/13/2020 - 02:04
This study aimed to explore the antimicrobial effects of activated lactoferrin (ALF) and rosemary extract (RE) on Escherichia coli O157:H7, Salmonella Enteritidis and Listeria monocytogenes, and to investigate their application potential in the meat storage. Minimum inhibitory concentrations (MICs) of ALF, RE and ALF–RE combinations were determined via microtiter plate assay. MICs of ALF were 1%...
Sun, 10/11/2020 - 22:22
In this review, we explore the plasmidome of A. salmonicida. Knowledge has accumulated about this diverse plasmidome, which can be separated into three categories (R‐plasmids, virulence plasmids, and cryptic plasmids). The advance of DNA sequencing technology has played an important role in the elucidation of complete sequences, giving greater clarity to study the roles and importance of plasmids...
Sat, 10/10/2020 - 22:17
Carvacrol has been recognized as an efficient growth inhibitor of food pathogens. However, carvacrol oil is poorly water‐soluble and can be oxidized, decomposed or evaporated when exposed to the air, light, or heat. To overcome these limitations, a carvacrol nanoemulsion was developed and its antimicrobial activity against food pathogens evaluated in this study. The nanoemulsion containing 3%...
Sat, 10/10/2020 - 06:27
Author(s): Shamsun Nahar, Angela Jie-won Ha, Kye-Hwan Byun, Md. Iqbal Hossain, Md. Furkanur Rahaman Mizan, Sang-Do Ha Food contamination is a major public health concern, with Salmonella Typhimurium, Escherichia coli, and Pseudomonas aeruginosa being the prominent causal agents. They often produce resistant shields in food through biofilm formation and are difficult to remove from food-contact...
Sat, 10/10/2020 - 06:22
Author(s): Xiaonan Zhao, Ming Hu, Qing Zhang, Cui Zhao, Yin Zhang, Lulu Li, Jing Qi, Yanbo Luo, Dong Zhou, Yuqing Liu Salmonella spp. are one of the most important foodborne bacterial pathogens in human beings and animals. This study aimed to analyze the prevalence and characterization of Salmonella from broilers in Shandong, China. A total of 67 Salmonella were recovered from 600 rectal swabs...
Fri, 10/09/2020 - 22:18
The loop‐mediated isothermal amplification (LAMP) technique was used to investigate six salmonella‐specific sequences for their suitability to serve as targets for the pathogen identification. Sequences selected for designing LAMP primers were genes invA, bcfD, phoP, siiA, gene62181533 and a region within the ttrRSBCA locus. Primers including single nucleotide polymorphisms were configured as...
Fri, 10/09/2020 - 22:18
HMP‐P synthase (Thi5) is a critical enzyme in the biosynthesis of thiamine pyrophosphate in yeast. A Thi5 homolog from Legionella pneumophila, which contributes to HMP synthesis in its native host, is an enzyme that binds pyridoxal‐5’‐phosphate and releases HMP when the purified protein is incubated with iron. In a heterologous system Thi5 from Saccharomyces cerevisiae and L. pneumophila have...
Fri, 10/09/2020 - 22:16
Concerning potential food safety and/or public health risks raised by viable but non‐culturable (VBNC) state bacteria, factors affecting its occurrence during thermosonication were summarised. The relative ratios of Salmonella and Escherichia coli in the VBNC state were higher than that of Staphylococcus aureus, suggesting that the tested gram‐negative bacteria would be more likely to survive in...
Fri, 10/09/2020 - 16:00
Molecular methods, particularly those based on real-time PCR (qPCR), have become a popular approach to detect pathogens in food samples. This technique may take advantage of hydrolysis fluorescent probes for increased specificity. Even though suitable, this approach loses the capacity of performing result confirmation by melt curve analysis. In the current study, we developed an alternative...
Thu, 10/08/2020 - 22:20
Co‐microencapsulation of lactobacilli cell free and propolis extract inhibited bacterial growth considerably. Abstract Cell free extracts (CFE) obtained from Lactobacillus plantarum FI 8595 and Lactobacillus reuteri ATCC 55730 alone or in combination with propolis ethanolic or water extracts (1%) were microencapsulated with maltodextrin (25%) before the subsequent spray drying process. They were...
Thu, 10/08/2020 - 22:17
Recent studies have shown that Salmonella shedding status affects sows’ microbiota during gestation and that these modifications are reflected in the faecal microbiota of their piglets at weaning. The aims of this study were: 1) to evaluate the persistence, up to the fattening period, of the previously measured link between the microbiota of piglets and their mothers’ Salmonella shedding status;...
Thu, 10/08/2020 - 16:00
Salmonella enterica serovar Enteritidis is noted for its ability to survive the harsh antibacterial activity of egg white which is presumed to explain its occurrence as the major food-borne pathogen associated with the consumption of eggs and egg products. Liquid egg white is a major ingredient for the food industry but, because of its thermal fragility, pasteurization is performed at the modest...
Thu, 10/08/2020 - 16:00
Salmonella enterica is a common foodborne illness in the United States and globally. An increasing number of Salmonella infections are resistant to antibiotics, and many of the genes responsible for those resistances are carried by plasmids. Plasmids are important mediators of horizontal gene exchange, which could potentially increase the spread of antibiotic resistance (AR) genes. Twenty-eight...
Thu, 10/08/2020 - 06:23
Author(s): S. Vaddu, J. Kataria, E.N. Rama, A.E. Moller, A. Gouru, M. Singh, H. Thippareddi Peroxy acetic acid (PAA) is widely used as an antimicrobial in poultry processing, specifically in the chiller. While the natural pH of PAA at the concentrations used is between 4.5 and 6.0, poultry processors adjust the pH to ≥8.0 to maintain product yield. The objective of this study was to evaluate (i)...
Thu, 10/08/2020 - 06:23
Author(s): Yang He, Yanyan Yang, Yuanyang Dong, Koichi Ito, Bingkun Zhang Salmonella Typhimurium (S. Typhimurium) infection in broiler chickens threatens public health and livestock production. In this study, we explored the effects of highly nutritious (crude protein 21.8%, metabolizable energy 3.16 Mcal/kg) and lowly nutritious (crude protein 18.1%, metabolizable energy 2.98 Mcal/kg) diets on S...
Thu, 10/08/2020 - 02:03
Salmonella is a well-known food-borne pathogen causing disease in humans and animals worldwide. Peptidyl-prolyl isomerases (PPIases) catalyse the cis–trans isomerisation of prolyl bound, which is a slow and rate-limiting step of protein folding. Here, we present the biochemical and molecular characterisation of a novel multi-domain parvulin-type, PPIases-C from the pathogenic bacteria Salmonella...
Wed, 10/07/2020 - 16:00
S. Pullorum (Salmonella enterica serovar Gallinarum biovars Pullorum) is an infectious pathogen that causes the acute systemic disease called Pullorum disease in poultry. This disease causes huge losses to the poultry industry and seriously affects the yield and quality of the chicken product. It is not easily distinguishable with fowl typhoid caused by S. Gallinarum (Salmonella enterica serovar...
Wed, 10/07/2020 - 06:17
Author(s): Zahra Dehghani, Trieu Nguyen, Mohsen Golabi, Morteza Hosseini, Ali H. Rezayan, Javad Mohammadnejad, Anders Wolff, Aaydha C. Vinayaka Concentration of pathogens directly from food samples by using magnetic beads is a potential strategy in the on-site food sample analysis. In this study, magnetic beads, double modified with platinum/palladium nanoparticle (Pt/Pd NP) and DNA aptamer, is...
Wed, 10/07/2020 - 06:16
Author(s): Long Chen, Xinyao Wei, Byron D. Chaves, David Jones, Monica A. Ponder, Jeyamkondan Subbiah The objectives of this study were to investigate the effects of processing parameters (relative humidity (RH), temperature, and exposure time) on the ethylene oxide (EtO) microbial inactivation of Salmonella spp. and to evaluate Enterococcus faecium NRRL B2354 as a suitable surrogate for...
Sun, 10/04/2020 - 02:04
Abstract Salmonella spp. can cause animal and human salmonellosis. In this study, we established a simple method to detect all Salmonella species by amplifying a specific region within the flgE gene encoding the flagellar hook protein. Our preliminary sequence analysis among flagella-associated genes of Salmonella revealed that although Salmonella Gallinarum and Salmonella Pullorum are lacking...
Sun, 10/04/2020 - 00:00
Salmonella enterica serovar Typhimurium (ST) remains a major infectious agent in the USA, with an increasing antibiotic resistance pattern, which requires the development of novel antimicrobials capable of controlling ST. Polyphenolic compounds found in plant extracts are strong candidates as alternative antimicrobials, particularly phenolic acids such as gallic acid (GA), protocatechuic acid (PA...
Fri, 10/02/2020 - 10:03
Biofilms formed by Salmonella enterica are a frequent source of food supply contamination. Since biofilms are inherently resistant to disinfection, new agents capable of preventing biofilm formation are needed. Synthetic analogs of 4-oxazolidinone containing natural products have shown promise as antibiofilm compounds against Gram-positive bacteria. The purpose of our study was 2-fold: to...
Fri, 10/02/2020 - 04:02
Salmonella is a foodborne pathogen commonly associated with poultry products. The aims of this work were to 1) estimate the impact of critical steps of the slaughter process on Salmonella detection from broiler chicken carcasses in two commercial poultry slaughter plants in Quebec, Canada, 2) investigate the presence of the pathogen in the slaughter plant environment, 3) describe, using a high...
Thu, 10/01/2020 - 10:09
The use of bacteriophages as antimicrobial agents represents a promising alternative for the control of pathogenic bacteria. Here, we present the complete genome sequences of two novel Salmonella enterica lytic bacteriophages, NBSal006 and NBSal007, candidates for Salmonella biocontrol.
Thu, 10/01/2020 - 06:35
Author(s): Stefani Machado Lopes, Danielle Carmo da Silva, Eduardo César Tondo Cured egg yolk is a novel gastronomic preparation, which a salt and sugar mixture gradually diffuses into the egg yolk, promoting its solidification from the exterior to the inner parts and greatly concentrates fat and flavor. This study was undertaken to analyze the effect of curing and its association with heat...
Thu, 10/01/2020 - 00:00
Salmonella spp. are recognized as important foodborne pathogens globally. Salmonella enterica serovar Rissen is one of the important Salmonella serovars linked with swine products in numerous countries and can transmit to humans by food chain contamination. Worldwide emerging S. Rissen is considered as one of the most common pathogens to cause human salmonellosis. The objective of this study was...
Tue, 09/29/2020 - 16:00
Salmonellosis is a common foodborne disease. We previously reported the protection of Caenorhabditis elegans from Salmonella Typhimurium DT104 infection by Lactobacillus zeae LB1. However, the mechanism is not fully understood. C. elegans exhibits behavior plasticity when presented with diverse pathogenic or commensal bacteria. Whether it can exert approach avoidance to S. Typhimurium through...
Sun, 09/27/2020 - 06:17
Author(s): Honghong Xian, Yu Yuan, Chao Yin, Zhenyu Wang, Ruoyun Ji, Chao Chu, Xinan Jiao, Qiuchun Li Salmonella Pathogenicity Island 19 (SPI-19) encoded type VI secretion system (T6SS) is a virulence factor present in few serotypes of S. enterica, including S. Dublin, S. Gallinarum and S. Pullorum. Comparative genomic sequence analysis revealed that the gene clusters of SPI-19 showed high...
Sun, 09/27/2020 - 06:15
Author(s): L.K. Froebel, L.E. Froebel, T. Duong The development of interventions to reduce human foodborne pathogens in the gastrointestinal (GI) tract of chickens will be important for improving the microbial food safety of poultry. Saccharomyces-derived prebiotic Refined Functional Carbohydrates (RFC), composed primarily of β-glucans, mannanoligosaccharides (MOS), and D-mannose, have been...
Sat, 09/26/2020 - 08:03
by Amanda Aparecida Seribelli, Marcelo Ferreira Cruz, Felipe Pinheiro Vilela, Miliane Rodrigues Frazão, Mario H. Paziani, Fernanda Almeida, Marta Inês Cazentini Medeiros, Dália dos Prazeres Rodrigues, Marcia R. von Zeska Kress, Marc W. Allard, Juliana Pfrimer Falcão

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