Salmonella

Fri, 03/27/2020 - 04:03
A large outbreak of extensively drug-resistant (XDR) Salmonella enterica serotype Typhi infections is ongoing in Pakistan, predominantly in Sindh Province. Here, we report the sequencing and characterization of five XDR Salmonella Typhi isolates from the Punjab province of Pakistan that are closely related to the outbreak strain and carry the same IncY plasmid.
Fri, 03/27/2020 - 04:03
Salmonella enterica subsp. enterica isolates are the leading cause of foodborne illness worldwide. Here, we report the draft genomes of 26 Salmonella isolates of food and clinical origin, belonging to four serovars, associated with outbreaks from 1999 to 2006 in the south of Brazil.
Fri, 03/27/2020 - 04:03
This is an announcement for the genome sequence of a clinical isolate of Salmonella enterica subsp. arizonae isolated from the urine and prostate of a 6-year-old male Labrador retriever. This is one of the few reports of a Salmonella enterica subsp. arizonae isolate cultured from canine urine.
Fri, 03/27/2020 - 04:03
Five strains of Salmonella enterica subsp. enterica serovar Infantis and two strains of S. enterica subsp. enterica serovar Kentucky isolated in 2017 from Ecuadorian layer poultry farms were sequenced using Illumina MiSeq technology. These isolates were collected on layer farms in central Ecuador, one of the most important areas of egg production in the country. The genome sequences of these...
Fri, 03/27/2020 - 04:03
We present here the complete genome sequences of three Salmonella enterica subsp. enterica serovar Muenchen strains, LG24, LG25, and LG26. All three strains were isolated from almond drupes grown in an orchard in San Joaquin County, California, in 2016. These genomic sequences are nonidentical and will contribute to our understanding of S. enterica genomics.
Fri, 03/27/2020 - 03:51
The combination of nisin, EDTA, and sulfite at a low dose of 0.625% exhibited higher antimicrobial activity than did a high dose of sulfite (1.25%) for shrimp preservation. Based on aerobic bacteria counts, psychrotrophic bacteria, and TVB‐N, shrimp treatment with combination of nisin, EDTA, and low‐dose sulfite were still acceptable within 10 days of storage. Abstract This study aims to...
Thu, 03/26/2020 - 03:34
Fosfomycin is gaining renewed interest for treating urinary tract infections. Monitoring fosfomycin resistance is therefore important in order to detect the emergence of novel resistance mechanisms. Here, we used the Rapid Fosfomycin NP test to screen a collection of extended-spectrum-β-lactamase-producing Escherichia coli isolates from Switzerland and found a fosfomycin-resistant isolate in...
Thu, 03/26/2020 - 03:33
Author(s): Hongduo Bao, Yan Zhou, Khashayar Shahin, Hui Zhang, Fuliang Cao, Maoda Pang, Xuhui Zhang, Shujiao Zhu, Ademola Olaniran, Stefan Schmidt, Ran Wang S. Enteritidis continues to be the most common pathogen of farm animals and a major public health burden worldwide. Using bacteriophages is a potential alternative to antibiotics against S. Enteritidis infection. In this study, the genome...
Thu, 03/26/2020 - 03:32
Author(s): A.G. Ríos-Castillo, C. Ripolles-Avila, J.J. Rodríguez-Jerez The capacity of real-time PCR (RT-PCR), the VIDAS immunoassay system, and the conventional count method for detecting Salmonella enterica serovar Typhimurium and Listeria monocytogenes biofilm cells was evaluated in this study. After biofilm formation, tests were performed under different drying times (0, 6, 12, 24, and 72 h)...
Thu, 03/26/2020 - 03:32
Author(s): H.W. Kim, M.S. Rhee This study aimed to develop a pasteurization method against Salmonella enterica serovar Typhimurium in orange juice using low concentrations of naturally derived antimicrobials, β-resorcylic acid and capric acid, under mild temperature conditions based on their synergistic bactericidal interactions. Response surface methodology was used to construct a model based on...
Thu, 03/26/2020 - 03:27
The aim of the study was to assess the antibacterial properties of bioactive compounds released during the fermentation of goat’s milk by selected bacterial strains that are part of the kefir grain microflora. The material used in the experiments was kefir grain microflora (Lactobacillus kefiranofaciens subsp. kefirgranum DSM 10550, Lactobacillus kefiri PCM 2501, Lactobacillus parakefiri DSM...
Wed, 03/25/2020 - 03:33
While alternating between insects and mammals during its lifecycle, Yersinia pestis, the flea transmitted bacterium that causes plague, regulates its gene expression appropriately to adapt to these two physiologically disparate host environments. In fleas competent to transmit Y. pestis, low GC content genes y3555, y3551 and y3550 are highly transcribed, suggesting that these genes have a highly...
Wed, 03/25/2020 - 03:33
Nontyphoidal Salmonella species are globally disseminated pathogens and are the predominant cause of gastroenteritis. The pathogenesis of salmonellosis has been extensively studied using in vivo murine models and cell lines, typically challenged with Salmonella enterica serovar Typhimurium. Although S. enterica serovars Enteritidis and Typhimurium are responsible for most of the human infections...
Wed, 03/25/2020 - 03:33
In Enterobacteriales, the AcrAB-TolC efflux pump exports substrates, including antimicrobials, from the cell. Overexpression of AcrAB-TolC can occur after exposure to fluoroquinolones, leading to multidrug resistance. The expression of AcrAB-TolC in Salmonella is primarily regulated by the transcriptional activator RamA. However, other transcriptional activators, such as MarA, SoxRS, and Rob, can...
Thu, 03/19/2020 - 03:34
Contaminated vegetable seeds have been identified as a potential source of foodborne bacterial pathogens.  This study was undertaken to observe the behavior of Salmonella and enterohemorrhagic Escherichia coli (EHEC) on vegetable seeds, contaminated by direct contact with artificially inoculated soil, during germination.  Sterile sandy soil inoculated with lyophilized cells of four individual...
Thu, 03/19/2020 - 03:33
Escherichia coli O157:H7 and Salmonella enterica are leading causes of foodborne outbreaks linked to fresh produce. Both species can enter the "viable but nonculturable" (VBNC) state that precludes detection using conventional culture-based or molecular methods. In this study, we assessed propidium monoazide-quantitative PCR (PMA-qPCR) assays and novel methods combining PMA and loop-mediated...
Wed, 03/18/2020 - 03:26
Background Typhoid fever remains a major cause of morbidity and mortality in low- and middle-income settings. In the last 10 years, several reports have described the reemergence of typhoid fever in southern and eastern Africa, associated with multidrug-resistant H58 Salmonella Typhi. Here, we identify risk factors for pediatric typhoid fever in a large epidemic in Blantyre, Malawi. Methods A...
Tue, 03/17/2020 - 03:33
Author(s): Lina Sheng, Zheng Zhang, Gang Sun, Luxin Wang Due to the lack of thermal inactivation steps, the fresh produce industry is actively looking for new, environmentally friendly and effective non-thermal control strategies to eliminate foodborne pathogens on produce surfaces and to prevent cross-contamination. Photodynamic inactivation of bacteria has emerged as a novel alternative...
Mon, 03/16/2020 - 03:28
Salmonella typhimurium is able to form biofilms as a resistance mechanism against antimicrobials; therefore, it represents a problem for assuring food safety and highlights the importance of research on anti‐biofilm technologies. In this study, S. typhimurium biofilms were inactivated with the combination of clove essential oil (CEO) and ultraviolet light (UV‐C). The volatile composition of the...
Sun, 03/15/2020 - 04:01
Author(s): Taekeon Jung, Yugyung Jung, Jaehyeon Ahn, Sung Yang Most biosensors use small amounts (a few tens of microliters) of a sample to detect bacteria. However, it is necessary to detect bacteria with at least a few milliliters of the sample to obtain statistically significant results. Therefore, the bacteria concentration process is critical for the rapid and accurate detection of bacteria...
Sun, 03/15/2020 - 03:57
Author(s): S. Kumar, M. Singh, D.E. Cosby, N.A. Cox, H. Thippareddi Poultry processors use antimicrobials to reduce the risk of pathogens on poultry and poultry products. Efficacy of selective and nonselective plating media to enumerate injured Salmonella (selective media—brilliant green sulfa agar and Petrifilm Enterobacteriaceae Plate Count; nonselective media—tryptic soy agar and Petrifilm...
Sat, 03/14/2020 - 03:30
Author(s): Pratima Adhikari, Sudhir Yadav, Douglas E. Cosby, Nelson A. Cox, Joshua A. Jendza, Woo Kyun Kim Feed additives can be alternatives to antibiotics for routinely encountered pathogens in the poultry production. The objective of this study was to understand effects of organic acid mixture on growth parameters and Salmonella Typhimurium (ST) colonization in broilers. Organic acid mixture...
Fri, 03/13/2020 - 03:33
Salmonella enterica subsp. enterica serovar Enteritidis has been the prevalent serovar isolated from gastroenteritis cases in Brazil since the 1990s. Here, we report the draft genomes of 30 S. Enteritidis isolates originating from a variety of patients and implicated foods during outbreaks between 1999 and 2006 in Brazil.
Fri, 03/13/2020 - 03:24
Chagas disease, caused by the hemoflagellate protist Trypanosoma cruzi, affects nearly 6 million people worldwide, mainly in Latin America. Hematophagous triatomine insects (“kissing bugs”) are the primary vectors of T. cruzi throughout the Americas and feed on a variety of animals, including humans. Control of triatomines is central to the control of T. cruzi infection. Recent advances in...
Fri, 03/13/2020 - 03:24
The enteric pathogen Salmonella enterica can interact with parts of the plant immune system despite not being a phytopathogen. Previous transcriptomic profiling of S. enterica associating with tomato suggested that Salmonella was responding to oxidative and nitrosative stress in the plant niche. We aimed to investigate whether Salmonella was eliciting generation of reactive oxygen species (ROS)...
Thu, 03/12/2020 - 03:36
by Lene Bungenstock, Amir Abdulmawjood, Felix Reich With regards to the frequently reported findings of spoilage bacteria and pathogens in various foods there is a need to explore new ways to control hazards in food production and to improve consumer safety. Fermented sausages from traditional and industrial production in Germany were screened for lactic acid bacteria with antibacterial effects...
Thu, 03/12/2020 - 03:32
Author(s): Beibei Zhang, Guang Li, Muhammad Suhaib Shahid, Liping Gan, Hao Fan, Zengpeng Lv, Shaojia Yan, Yuming Guo This study was conducted to investigate the effects of dietary arginine (Arg) supplementation on the inflammatory response and gut microbiota of broiler chickens subjected to Salmonella enterica serovar Typhimurium. One hundred and forty 1-day-old Arbor Acres male birds were...
Thu, 03/12/2020 - 03:32
Author(s): G.K. Walker, M.M. Suyemoto, L.B. Borst, J. Brake The genetic relatedness and antimicrobial susceptibility profiles of Salmonella isolated from poultry and their environment were determined. One broiler breeder flock (BBF1) and 2 broiler flocks (BF1 and BF2) were reared over a 1.75-year period on the same poultry research farm. Hatching eggs were obtained from BBF1 to produce BF1 chicks...
Wed, 03/11/2020 - 03:35
ABSTRACT The reactive intermediate deaminase RidA (EC 3.5.99.10) is conserved across all domains of life and deaminates reactive enamine species. When Salmonella enterica ridA mutants are grown in minimal medium, 2-aminoacrylate (2AA) accumulates, damages several pyridoxal 5'-phosphate (PLP)-dependent enzymes, and elicits an observable growth defect. Genetic studies suggested that damage to...
Wed, 03/11/2020 - 03:29
In this study, a novel dual priming oligonucleotide (DPO) system‐based polymerase chain reaction (PCR; DPO system‐based PCR) assay, which detected the fimY gene of Salmonella, was developed for the fast food testing. The DPO system‐based PCR assay allowed a wide range of annealing temperatures at 48–68°C to efficiently amplify fimY gene with an analytical detection limit of 1.2 × 102 CFU/ml for...
Wed, 03/11/2020 - 03:26
Reactive oxygen species (ROS) are small oxygen-derived molecules that are used to control infections by phagocytic cells. In macrophages, the oxidative burst produced by the NOX2 NADPH-oxidase is essential to eradicate engulfed pathogens by both oxidative and non-oxidative killing. Indeed, while the superoxide anion (O2-) produced by NOX2, and the other ROS derived from its transformation, can...
Tue, 03/10/2020 - 03:25
M. Jiang et al. In July 2018, an outbreak of 10 cases of Salmonella enterica serovar Enteritidis infection occurred in Shenzhen, China. Outbreak investigation complemented by whole-genome sequencing traced the source to food ordered online. Our investigation highlights the role of online food delivery platforms as a new mode of foodborne disease transmission.
Tue, 03/10/2020 - 03:25
Bacterial resistance to antibiotics is a major concern worldwide, leading to an extensive search for alternative drugs. Promising candidates are antimicrobial peptides (AMPs), innate immunity molecules, shown to be highly efficient against multidrug resistant bacteria. Therefore, it is essential to study bacterial resistance mechanisms against them. For that purpose, we used experimental...
Tue, 03/10/2020 - 03:25
Dairy cows are known reservoirs of Salmonella enterica and human salmonellosis has been attributed to the consumption of contaminated dairy and beef products as well as poultry meat and eggs. Although many S. enterica serovars are known to colonize the gastrointestinal tract of cattle, the interactions between dairy commensal (or persistent) and transient Salmonella serovars with bovine...
Mon, 03/09/2020 - 03:59
LWT
Author(s): G. Bhuvana Priya, Ravi Kant Agrawal, A. Arun Prince Milton, Madhu Mishra, S.K. Mendiratta, Ashish Luke, Sofia Inbaraj, Bhoj Raj Singh, Deepak Kumar, Ravi Kumar GVPPS, Swaraj Rajkhowa Salmonella, recognized as one of the most important foodborne bacteria has worldwide health and socioeconomic impact. To prevent the occurrence of outbreaks and recall of food, a simple, rapid and robust...
Mon, 03/09/2020 - 03:46
In this study, a real-time loop-mediated isothermal amplification (Rti-LAMP) combined with immunomagnetic beads separation (IMS) assay and SDS rinse was developed for the rapid and sensitive detection of Salmonella in ready-to-eat (RTE) meat without prior enrichment. Polyclonal antibody and specific immunomagnetic beads (IMBs) for Salmonella flagellum were prepared. The capture efficiency was...
Sun, 03/08/2020 - 04:26
ABSTRACT Current study aims to characterize the influence of sub-minimum inhibitory concentration (sub-MIC) of ciprofloxacin on Salmonella intracellular survival and host virulence. Herein, Salmonella resistance patterns to various antibiotics were in agreement with those reported in previous studies. Moreover, intracellular survival of both ciprofloxacin-sensitive and -resistant Salmonella was...
Sat, 03/07/2020 - 03:28
ABSTRACT Nondairy fermented products, such as fruit and vegetable juices, are gaining popularity among consumers worldwide for health‐related and economic reasons. The purpose of this study was to determine the changes in microbiological quality; antimicrobial and antioxidant activity; and phenolic, vitamin C, minerals, and cadmium content occurring during spontaneous fermentation of curly kale...
Sat, 03/07/2020 - 03:24
We previously described how four deleterious synonymous mutations in the Salmonella enterica rpsT gene (encoding ribosomal protein S20) result in low S20 levels that can be compensated by mutations that restore [S20]. Here, we have further studied the cause for the deleterious effects of S20 deficiency and found that the S20 mutants were also deficient in four other 30S proteins (S1, S2, S12, and...
Thu, 03/05/2020 - 03:37
ABSTRACT Integrative mobilizable elements belonging to the SGI1-H, -K, and -L Salmonella genomic island 1 (SGI1) variant groups are distinguished by the presence of an alteration in the backbone (IS1359 replaces 2.8 kb of the backbone extending from within traN [S005] to within S009). Members of this SGI1-HKL group have been found in Salmonella enterica serovars and in Proteus mirabilis. Two...
Thu, 03/05/2020 - 03:34
Author(s): Gabriella Mendes-Oliveira, Tony Z. Jin, Osvaldo H. Campanella This study groups together pulse electric filed variables such as electric field intensity, pulse repetition rate, treatment time, and electrical conductivity of food in a single integrated energy density parameter and correlate it with the inactivation rate of Escherichia coli O157:H7 and Salmonella Typhimurium. A...
Thu, 03/05/2020 - 03:27
This study was designed to investigate the prevalence and characteristics of Salmonella in three Chinese pig abattoirs (A, B, and C) in Wuhan city in 2016. Four types of pig samples were collected and cultured for Salmonella. Salmonella was detected from 329 samples among the 1440 tested (22.9%). There was no significant difference in the overall prevalence between the first visit and the second...
Wed, 03/04/2020 - 04:01
Author(s): Amal Senevirathne, Chamith Hewawaduge, John Hwa Lee Salmonella Enteritidis (SE) is one of the most common culprits of foodborne disease in humans due to its horizontal transmission from infected animals to humans. The development of a safe vaccine against Salmonella would be important for both farm animals and humans concerning disease containment. The SE ghosts carrying FliC were...
Wed, 03/04/2020 - 03:58
Worldwide emergence of Salmonella enterica serovar Newport (S. Newport) infection in humans, in parallel with a significant increasing prevalence of antimicrobial resistance (AR), is a serious public health concern. However, the prevalence of S. Newport resistance in China remains largely unknown. A retrospective study of 287 S. Newport clinical isolates collected during 1997–2018 was undertaken...
Wed, 03/04/2020 - 03:51
Fresh fruits and vegetables have numerous benefits to human health. Unfortunately, their consumption is increasingly associated with food‐borne diseases, Salmonella enterica being their most frequent cause in Europe. Agricultural soils were postulated as reservoir of human pathogens, contributing to the contamination of crops during the growing period. Since the competition with the indigenous...
Wed, 03/04/2020 - 01:19
Restaurant inspections seek to identify and correct risk factors for foodborne illness, but restaurant inspection data are not typically used more broadly as a food safety surveillance tool. In 2015, there was an outbreak of Salmonella serotype Newport infections associated with multiple restaurants in a chain (Chain A), primarily in Minnesota. The outbreak was associated with tomatoes that were...
Wed, 03/04/2020 - 01:19
The microbiological quality of ready-to-eat (RTE) foods from school cafeterias in Chongqing, China, was evaluated in comparison to a guideline published by a provincial health commission. Based on their preparation processes and potential risks, the RTE foods were divided into 5 types: food type 1, general cooked and hot-held foods; food type 2, cooked meats; food type 3, heated aquatic products...
Wed, 03/04/2020 - 01:19
Environmental testing for  Salmonella  Enteritidis is required for U.S. shell egg producers with > 3,000 hens on a farm. The egg producer assumes all costs for the mandatory testing. According to the FDA Egg Rule, either manure scraper or drag swabs can be collected according to published guidelines and requirements. The current study was undertaken to determine the efficacy of  Salmonella  ...
Wed, 03/04/2020 - 01:19
A study was undertaken to examine hygienic control of the slaughter and dressing process for beef cattle at Australian export processing establishments. Samples were collected from two points during the process: immediately after hide removal and at the completion of dressing before the commencement of chilling. Hindquarter (HQ) and forequarter (FQ) samples were collected from 24 establishments,...
Wed, 03/04/2020 - 01:19
The economic burden of foodborne illness has been estimated to be as high as $90 billion annually.  For policy purposes, it is often important to understand not just the overall cost of illness, but also the costs associated with individual products or groups of products. In this study, I estimate the cost of foodborne illnesses from 29 pathogens associated with non-game meat and poultry products...

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