Research Publications (Food Safety)

Developing sensitive assay for pathogen detection is a sustained demand for food scientists, industries, and government. In this study, an ultrasensitive colorimetric lateral flow assay (LFA) was successfully developed for the detection of Salmonella typhimurium (S. typhimurium). Based on cascade enzyme reaction induced by horseradish peroxidase (HRP)-encapsulated starch-based liposome, the sensitivity of the conventional LFA can be dramatically improved.

Zoonoses and Public Health, EarlyView. Objective We describe the epidemiology of live poultry-associated salmonellosis (LPAS) and investigate potential risk factors associated with hospitalization among adults aged ≥65 years in the United States during 2008–2017.

This study aimed to develop a combination of immunomagnetic separation (IMS) and real-time PCR (qPCR) techniques to detect and analyze Salmonella spp. (SAL), Listeria monocytogenes (LM), and Escherichia coli O157:H7 (O157: H7) in meat samples quickly and accurately. Bacteria-specific immunomagnetic beads (IMBs) were prepared from carboxyl magnetic beads and affinity-purified polyclonal antibodies using a novel magnetic bead activator.

Translational fidelity is maintained by multiple quality control steps in all three domains of life. Increased translational errors (mistranslation) occur due to genetic mutations and external stresses. Severe mistranslation is generally harmful, but moderate levels of mistranslation may be favored under certain conditions. To date, little is known about the link between translational fidelity and host–pathogen interactions.

Journal of Applied Microbiology, Volume 132, Issue 2, Page 872-889, February 2022. This systematic review focuses on obtaining the most relevant information from multiple studies that detected a mobilized colistin resistance mcr gene in Salmonella for a better comprehension of its global distribution.

Journal of Applied Microbiology, Volume 132, Issue 2, Page 1503-1517, February 2022. This study evaluated detection methods for Salmonella Typhi (S.

Journal of Applied Microbiology, Volume 132, Issue 2, Page 1449-1456, February 2022. Aims We investigated the fate of Salmonella in lettuce seeds grown in a hydroponic system and the potentials of applying photodynamic inactivation (PDI) to enhance microbial safety of hydroponic farming systems. Methods and Results Lettuce was grown from Salmonella-contaminated seeds, and rose bengal-mediated PDI was applied.

Our previous survey revealed a poor microbial quality of, and the presence of Salmonella on leafy green vegetables grown/sold in Accra, Ghana.  This study validated the efficacy of some cleaning/sanitation methods commonly used by Ghanaian households (tap water, salt solution, lemon juice, and vinegar), by comparing them with the performance of several sanitation approaches used by the U.S.

Foods contaminated by foodborne pathogens have always been a great threat to human life. Herein, we constructed an electrochemical immunosensor for Salmonella detection by using a Fe3O4@graphene modified electrode.

Dairy cows can be reservoirs of foodborne pathogens such as Salmonella that pose serious public health risks to humans.

Background A wide variety of pathogens can cause disease in humans via consumption of contaminated food.

Foodborne diseases affect an estimated 600 million people worldwide annually, with the majority of these illnesses caused by Norovirus, Vibrio, Listeria, Campylobacter, Salmonella, and Escherichia coli. To elicit infections in humans, bacterial pathogens express a combination of virulence factors and toxins.

Animal and food sources are seen as a potential transmission pathway of antimicrobial resistance (AMR) to humans. The aim of this study is to describe Campylobacter, Salmonella, and commensal Escherichia coli multi-drug resistance (MDR) in the food chain between 2014 and 2019 in Portugal. AMR surveillance data from food-producing animals and food were assessed. MDR relative frequencies were estimated by bacterial genus and year.

A collection of human-epidemiologically unrelated S. enterica strains collected over a 3-year period (2016 to 2018) in Italy by the national surveillance Enter-Net Italia was analysed. Methods: Antimicrobial susceptibility tests, including the determination of minimal inhibitory concentrations (MICs) for colistin, were performed. Colistin resistant strains were analysed by PCR to detect mobile colistin resistance (mcr) genes. In mcr-negative S.

Intestinal epithelium provides the largest barrier protecting mammalian species from harmful external factors; however, it can be severely compromised by the presence of bacteria in the gastrointestinal (GI) tract. Antibiotics have been widely used for the prevention and treatment of GI bacterial infections, leading to antimicrobial resistance in human and veterinary medicine alike.

Antibiotics are widely used in intensive fish farming, which in turn increases the emergence of antimicrobial-resistant (AMR) bacteria in the aquatic environment. The current study investigates the prevalence and determines the antimicrobial susceptibility of E. coli, Salmonella, and Vibrio in farmed fishes on the west coast of Peninsular Malaysia. Over a period of 12 months, 32 aquaculture farms from the Malaysian states of Selangor, Negeri Sembilan, Melaka, and Perak were sampled. Both E.

Zoonoses and Public Health, EarlyView. In December 2018, PulseNet, the national laboratory network for enteric disease surveillance, identified an increase in Salmonella Typhimurium isolates with an uncommon pulsed-field gel electrophoresis pattern which was previously isolated from hedgehogs.

Pullorum disease caused by Salmonella Pullorum remains an important disease for the poultry industry due to high morbidity and mortality in many countries. Phage therapy is becoming an alternative strategy to control multidrug-resistant Salmonella infections in young chicks. However, how bacteriophages affect the growth performance of chicks infected with S. Pullorum remains poorly understood.

Salmonella type III secretion system (T3SS) effector SseK3 is a glycosyltransferase delivered directly into the host cells to modify host protein substrates, thus manipulating host cellular signal transduction. Here, we identify and characterize the Arg-GlcNAcylation activity of SseK3 inside bacterial cells.

As part of the National Antimicrobial Resistance Monitoring System (NARMS) activities, the United States Department of Agriculture (USDA) Food Safety Inspection Service (FSIS) collected cecal samples from food animal slaughter facilities throughout the country between 2014 and 2018. Of the 26,780 cecal samples from cattle, swine, chicken and turkey , 6,350 (23.71%) tested positive for Salmonella .

Introduction The objectives of this study were to evaluate the synergistic antimicrobial effect of ultraviolet A (UVA) and malic acid (MA) combination treatment against Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes on spinach and to elucidate the mechanisms underlying the synergistic antimicrobial effects. Methods The cocktail of three pathogens was inoculated on the spinach leaves.

The antibacterial and antibiofilm activity of essential oil of clove against Listeria monocytogenes and Salmonella Enteritidis were investigated. The chemical composition of the oil was characterized by gas chromatography–mass spectrometry. Stock solution of the essential oil of clove was prepared in 95% (v/v) ethanol (EOC). The antibacterial assays were performed by disk diffusion assay and minimal inhibitory concentration (MIC).

Zingiber officinale essential oil (ZEO) was encapsulated in chitosan nanoparticles at different concentrations using the emulsion-ionic gelation technique and its antioxidant and antibacterial effects were investigated. The results indicated that ZEO level had a significant effect on encapsulation efficiency (EE), loading capacity (LC), particle size and zeta potential.

The aim of the present study was to investigate the detrimental effect of ultrasound application, as an alternative to pasteurization, on raw goat milk microorganisms and some food pathogens including Brucella melitensis. For this purpose, six different ultrasound applications with a power of 20 kHz at 100%, 50% and 10% amplitudes with or without pulsation were practiced.

Journal of Food Science, EarlyView. Hummus is a popular dip in the Middle East region prepared by mixing the boiled chickpeas with tahini and other ingredients, and because its consumption has increased world-wide some notoriety has developed following an increase in the incidence of hummus-related illness outbreaks and recalls.