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Shigella

Thu, 05/16/2019 - 11:01
Microcin PDI (MccPDI), a class IIa microcin that is produced by Escherichia coli strains 25 and 284, is known to inhibit foodborne pathogenic enterohemorrhagic E. coli serotypes O157:H7 and O26. Here we demonstrate that MccPDI can inhibit Shigella strains and E. coli isolates that are multidrug resistant, the latter including strains known to cause urinary tract infections in people and companion...
Thu, 05/16/2019 - 01:08
Publication date: Available online 13 May 2019Source: LWTAuthor(s): Seyed Mohammad Bagher Hashemi, Reza Roohi, Mohammad Reza Mahmoudi, Daniel GranatoAbstractInactivation of Listeria monocytogenes (serotype 4 ab), Shigella sonnei, Byssochlamys fulva and Saccharomyces cerevisiae and the degradation of β-carotene and ascorbic acid in tangerine juice subjected to pulsed-thermosonication were modeled...
Thu, 05/16/2019 - 01:07
Available online 15 May 2019 Author(s): Narges Elahi, Mohammad Hadi Baghersad, Mehdi KamaliAbstractEarly detection of infectious bacteria is a necessity for combating infectious diseases. Due to low infectious dose of Shigella, rapid and sensitive detection is needed. Compared to the presented genes, Spa gene can be introduced as a novel sequence for all species of Shigella detection. Herein, the...
Sat, 05/11/2019 - 10:14
Abstract Aim Intrauterine growth retardation (IUGR) is a prevalent problem in mammals. The present study was conducted to unveil the alterations in intestinal microbiota in IUGR piglets. Methods and Results We identified the alterations of small intestinal microbiota in IUGR piglets on 7, 21, and 28 days of age using 16S rRNA sequencing. The results showed that IUGR piglets had a decreased...
Sun, 05/05/2019 - 01:14
Available online 2 May 2019 Author(s): Hiroki Maruoka, Atsushi Hinenoya, Noritomo Yasuda, Atsuyoshi Takeda, Shin Inoue, Tomoko Sumi, Kazuaki Koitabashi, Hiroshi Yasue, Kazuhiko Kogou, Shinji YamasakiAbstractWe developed a new GeneFields® EHEC/SS PCR dipstick DNA chromatography kit for the simultaneously detection of invA, ipaH, and stx genes in Salmonella enterica (56 strains), Shigella spp. (44...
Fri, 05/03/2019 - 01:13
Available online 30 April 2019 Author(s): R.M. Subasinghe, A.D. Samarajeewa, R. Scroggins, L.A. BeaudetteAbstractIdentification of bacteria in new or existing commercial microbial-based products (MBPs) is important for compliance with government regulations and for human and environmental risk assessment. Research was performed to develop effective methods to identify bacteria present in a MBP...
Sun, 04/28/2019 - 20:00
Shigella flexneri has an extremely complex genome with a significant number of virulence traits acquired by mobile genetic elements including bacteriophages and plasmids. S. flexneri serotype 1c is an emerging et...
Thu, 04/25/2019 - 01:12
Available online 20 April 2019 Author(s): Hang Wang, Xiaojie Qin, Si Mi, Xia Li, Xiaoling Wang, Weijiang Yan, Chunhui ZhangAbstractThe processing environment of broiler processing plants is a potential major source of bacterial contamination of broiler carcasses. This study investigated the effect of processing water and processing time on the microbial diversity of yellow-feathered broiler...
Wed, 04/24/2019 - 06:33
Scope Understanding biological functions of different free human milk oligosaccharides (HMOs) in shaping gastrointestinal tract microbiota during infancy is of great interest. We examined a link between HMOs in maternal milk and infant faecal microbiota composition and investigated the role of microbiota in degrading HMOs within the GI tract of healthy, breastfed, one‐month old infants. Methods...
Tue, 04/16/2019 - 20:00
Structural and Proteomic Changes in Viable but Non-culturable Vibrio cholerae Susanne Brenzinger1*, Lizah T. van der Aart1, Gilles P. van Wezel1, Jean-Marie Lacroix2, Timo Glatter3 and Ariane Briegel1* 1Department of Microbial Biotechnology & Health, Institute of Biology Leiden, Leiden University, Leiden, Netherlands 2Unité de Glycobiologie Structurale et Fonctionnelle, UMR CNRS 8576...
Wed, 04/10/2019 - 20:00
Campylobacter jejuni Demonstrates Conserved Proteomic and Transcriptomic Responses When Co-cultured With Human INT 407 and Caco-2 Epithelial Cells Nicholas M. Negretti1, Geremy Clair2, Prabhat K. Talukdar1, Christopher R. Gourley1, Steven Huynh3, Joshua N. Adkins2, Craig T. Parker3, Colby M. Corneau1 and Michael E. Konkel1* 1School of Molecular Biosciences, College of Veterinary Medicine,...
Tue, 04/09/2019 - 20:00
O-Antigen Gene Clusters of Plesiomonas shigelloides Serogroups and Its Application in Development of a Molecular Serotyping Scheme Daoyi Xi1,2,3,4†, Xiaochen Wang1,2,3,4†, Kexin Ning1,2,3,4, Qian Liu1,2,3,4, Fuyi Jing1,2,3,4, Xi Guo1,2,3,4 and Boyang Cao1,2,3,4* 1Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, TEDA College, Nankai University...
Tue, 04/09/2019 - 00:00
Toxins, Vol. 11, Pages 212: Roles of Shiga Toxins in Immunopathology Toxins doi: 10.3390/toxins11040212 Authors: Moo-Seung Lee Vernon L. Tesh Shigella species and Shiga toxin-producing Escherichia coli (STEC) are agents of bloody diarrhea that may progress to potentially lethal complications such as diarrhea-associated hemolytic uremic syndrome (D+HUS) and neurological disorders. The...
Mon, 04/08/2019 - 20:00
Gamma Irradiation Influences the Survival and Regrowth of Antibiotic-Resistant Bacteria and Antibiotic-Resistance Genes on Romaine Lettuce Vaishali Dharmarha1, Giselle Guron2, Renee R. Boyer1, Brendan A. Niemira3, Amy Pruden2, Laura K. Strawn1 and Monica A. Ponder1* 1Department of Food Science and Technology, Virginia Tech, Blacksburg, VA, United States 2Department of Civil and Environmental...
Mon, 04/01/2019 - 12:01
Summary Early gut microbial colonization is important for postnatal metabolic and immune development. However, little is known about the effects of different feeding modes (suckling versus bottle‐feeding) or microbial sources on this process in farm animals. We found that suckled and bottle‐fed newborn lambs had their own distinct gut microbiota. Results from 16S rRNA gene sequencing and qPCR...
Mon, 04/01/2019 - 12:01
Summary Early gut microbial colonization is important for postnatal metabolic and immune development. However, little is known about the effects of different feeding modes (suckling versus bottle‐feeding) or microbial sources on this process in farm animals. We found that suckled and bottle‐fed newborn lambs had their own distinct gut microbiota. Results from 16S rRNA gene sequencing and qPCR...
Fri, 03/29/2019 - 09:54
The purpose of this study is to establish a rapid and accurate method based on multiplex polymerase chain reaction combined with suspension array xTAG (Flexible Sequence‐tagged, xTAG) technology to simultaneously detect Salmonella Typhimurium, Brucella spp., Bacillus cereus and Shigella spp. in raw milk. Summary The purpose of this study is to establish a rapid and accurate method based on...
Thu, 03/28/2019 - 11:00
Shigella spp. are a leading cause of human diarrheal disease worldwide, with Shigella flexneri being the most frequently isolated species in developing countries. This serogroup is presently classified into 19 serotypes worldwide. We report here a multicenter validation of a multiplex-PCR-based strategy previously developed by Q. Sun, R. Lan, Y. Wang, A. Zhao, et al. (J Clin Microbiol 49:3766...
Thu, 03/28/2019 - 11:00
Azithromycin (AZM) has been recommended by the American Academy of Pediatrics for the treatment of shigellosis in children. In this study, 502 Shigella species isolated between 2004 and 2014 were tested for AZM epidemiological cutoff values (ECV) by disk diffusion. AZM MICs and the presence of the macrolide resistance genes [erm(A), erm(B), erm(C), ere(A), ere(B), mph(A), mph(B), mph(D), mef(A),...
Mon, 03/25/2019 - 11:00
The enteric pathogen Shigella is one of the leading causes of moderate-to-severe diarrhea and death in young children in developing countries. Transformed cell lines and animal models have been widely used to study Shigella pathogenesis. In addition to altered physiology, transformed cell lines are composed of a single cell type that does not sufficiently represent the complex multicellular...
Mon, 03/25/2019 - 11:00
The enteric bacterium and intracellular human pathogen Shigella causes hundreds of millions of cases of the diarrheal disease shigellosis per year worldwide. Shigella is acquired by ingestion of contaminated food or water; upon reaching the colon, the bacteria invade colonic epithelial cells, replicate intracellularly, spread to adjacent cells, and provoke an intense inflammatory response. There...
Fri, 03/22/2019 - 11:00
Bacteria of the genus Shigella, consisting of 4 species and >50 serotypes, cause shigellosis, a foodborne disease of significant morbidity, mortality, and economic loss worldwide. Classical Shigella identification based on selective media and serology is tedious, time-consuming, expensive, and not always accurate. A molecular diagnostic assay does not distinguish Shigella at the species level...
Sun, 03/10/2019 - 01:13
Available online 9 March 2019 Author(s): Reinhard Sting, Tobias Eisenberg, Maja HrubenjaAbstractAnalyses of short subunit gene sequences have been established for taxonomic classification and identification of bacteria and fungi. To produce partial bacterial ribosomal 16S rRNA and rpoB and fungal ribosomal ITS/LSU gene sequences for DNA sequencing, real-time PCR assays supplemented with the...
Wed, 03/06/2019 - 11:00
We developed a multiplex PCR method based on multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) that was designed for the rapid typing of Escherichia coli and Shigella isolates. The method amplifies seven VNTRs and does not require a sequencing capillary or fluorescent dyes. The amplification products are simply loaded on a standard agarose gel for electrophoresis, and the...
Thu, 02/28/2019 - 11:11
Abstract Scope : The intake of resistant starch (RS) may attenuate the risks of chronic illness, including colorectal cancer. However, whether the attenuated functionality of RS is maintained in cooked meat products needs to be clarified. Methods and Results : The effects of a cooked mixture of lean pork meat and RS (MS) on the growth performance, lipid accumulation and intestinal health of...
Tue, 02/19/2019 - 11:27
Prophage mediated horizontal gene transfer (HGT) plays a key role in the evolution of bacteria, enabling access to new environmental niches, including pathogenicity. Citrobacter rodentium is a host-adapted intestinal mouse pathogen and important model organism for attaching and effacing (A/E) pathogens including the clinically significant enterohaemorrhagic (EHEC) and enteropathogenic (EPEC)...
Sat, 02/09/2019 - 00:00
Abstract A gastroenteritis outbreak occurred in a university in May, 2017, Wuhan, China. The epidemiological survey and pathogen analysis were conducted to identify the pathogen and control this outbreak. Feces or anal swabs from individuals, water, and swabs taken from tap surfaces of the secondary water supply system (SWSS) and foods were collected for the detection of viruses...
Fri, 02/08/2019 - 17:00
James B. Thissen, Nicholas A. Be, Kevin McLoughlin, Shea Gardner, Paul G. Rack, Michael H. Shapero, Raymond R. R. Rowland, Tom Slezak, Crystal J. Jaing Microarrays have proven to be useful in rapid detection of many viruses and bacteria. Pathogen detection microarrays have been used to diagnose viral and bacterial infections in clinical samples and to evaluate the safety of biological drug...
Thu, 02/07/2019 - 01:13
Available online 6 February 2019 Author(s): Grażyna Budryn, Elżbieta Klewicka, Joanna Grzelczyk, Ilona Gałązka-Czarnecka, Radosław MostowskiAbstractLegume seeds and sprouts are a rich source of phytoestrogens in the form of isoflavonoids. For the first time, lactic acid fermentation of four types of legume sprouts was used to increase the content of isoflavonoids and microbiological safety. After...
Thu, 02/07/2019 - 01:13
Available online 31 January 2019 Author(s): S. Deshaware, S. Gupta, R. Singhal, P.S. VariyarAbstractDebittered bitter gourd juice was subjected to gamma irradiation doses of 0, 0.25, 0.5, 0.75, 1, 1.5 and 2.5 kGy and thermal pasteurization at temperatures of 65, 75, 85 and 95°C. Shigella boydii was the most heat resistant pathogen tested (D10 of 42.8 s at 65 ⁰C) while Bacillus cereus was the most...
Mon, 02/04/2019 - 01:10
Available online 31 January 2019 Author(s): Mariana Bona, Pedro Henrique Medeiros, Ana Karolina Santos, Thiago Freitas, Mara Prata, Herlice Veras, Marília Amaral, Daniel Oliveira, Alexandre Havt, Aldo Ângelo LimaABSTRACTShigella/Enteroinvasive Escherichia coli (EIEC) pathotype is a major enteropathogen associated with diarrhea and malnutrition in children from developing countries. This study...
Sun, 01/27/2019 - 01:12
Available online 25 January 2019 Author(s): Hoa Van Ba, Hyun-Woo Seo, Pil-Nam Seong, Sun-Moon Kang, Soo-Huyn Cho, Yoon-Seok Kim, Beom-Young Park, Sung-Sil Moon, Se-Ju Kang, Yong-Min Choi, Jin-Hyoung KimAbstractThis study was conducted to detect and identify microbial populations on pig carcasses at different slaughtering stages and on retail pork cuts at 24 h after slaughter as well as to...
Sat, 01/26/2019 - 01:08
Available online 22 January 2019 Author(s): Shaohua Lin, Yingyan Han, Chunzi, Yunbo Luo, Wentao Xu, Hongxia Luo, Guofang PangAbstractThe dissipation kinetics of a combined use of procymidone and thiamethoxam, and their impact on the biodiversity and pathogen on surface of tomatoes were studied. The half-lives of procymidone and thiamethoxam, used either on their own or in combination with each...
Fri, 01/25/2019 - 01:13
Publication date: Available online 23 January 2019 Innovative Food Science & Emerging TechnologiesAuthor(s): Seyed Mohammad Bagher Hashemi, Reza RoohiAbstractA comprehensive 3D, transient-free convection, and multiphase model was developed to determine the performance of ohmic heating (150, 200 and 250 V; 120 s; 99.4 °C) and conventional heating (90 °C; 15 min) for the inactivation of...
Mon, 01/14/2019 - 17:00
Jessica Guzman-Otazo, Lucia Gonzales-Siles, Violeta Poma, Johan Bengtsson-Palme, Kaisa Thorell, Carl-Fredrik Flach, Volga Iñiguez, Åsa Sjöling Water borne diarrheal pathogens might accumulate in river water and cause contamination of drinking and irrigation water. The La Paz River basin, including the Choqueyapu River, flows through La Paz city in Bolivia where it is receiving sewage, and...
Fri, 12/14/2018 - 01:11
Chaojie Yang, Haiying Wang, Hui Ma, Renlong Bao, Hongbo Liu, Lang Yang, Beibei Liang, Leili Jia, Jing Xie, Ying Xiang, Nian Dong, Shaofu Qiu, Hongbin Song
Sat, 12/08/2018 - 01:11
Available online 6 December 2018 Author(s): Sinosh Skariyachan, Sanjana GovindarajanAbstractThe present study elucidates biopreservation potential of an antimicrobial protein; bacteriocin, producing Pediococcus spp. isolated from dairy sample and enhancement of their shelf life in comparison with two chemical preservatives. The antimicrobial protein producing Pediococcus spp. was isolated from...
Fri, 12/07/2018 - 17:00
Valeria Ciancarella, Luigi Lembo-Fazio, Ida Paciello, Anna-Karin Bruno, Sébastien Jaillon, Sara Berardi, Marialuisa Barbagallo, Shiri Meron-Sudai, Dani Cohen, Antonio Molinaro, Giacomo Rossi, Cecilia Garlanda, Maria Lina Bernardini Shigella spp. are pathogenic bacteria that cause bacillary dysentery in humans invading the colonic and rectal mucosa where they induce dramatic inflammation. Here,...
Fri, 12/07/2018 - 01:10
Publication date: February 2019 , Volume 148Author(s): Daphney Shiningeni, Percy Chimwamurombe, Renatus Shilangale, Jane MisihairabgwiAbstractTo determine the prevalence of pathogenic bacteria in street vended ready-to-eat meats in Windhoek, Namibia, a total of 96 street vended ready to eat meat samples were evaluated. Prevalences of 42%, 52%, 15%, 6% and 83% were observed for Escherichia coli,...
Thu, 12/06/2018 - 01:10
Publication date: October 2018 , Volume 308, Issue 7Author(s): Michael Berger, Petya Berger, Erick Denamur, Alexander Mellmann, Ulrich DobrindtAbstractThe highly virulent Escherichia coli O104:H4 isolate that caused a large outbreak in 2011 carries three plasmids. Out of these, only one, the IncI plasmid pO104_90 that encodes two extended spectrum beta-lactamases, can transfer itself by...
Wed, 11/28/2018 - 01:10
Available online 27 November 2018 Author(s): Junli Feng, Qing Shen, Jiajia Wu, Zhiyuan Dai, Yi WangAbstractShigella flexneri is one of the major food-borne pathogens in China. Herein, we reported the development of a colorimetric aptasensor based on aptamer and gold nanoparticles (AuNPs) for detection of Sh. flexneri. Aptamer with high binding affinity to Sh. flexneri was firstly immobilized on...
Thu, 11/01/2018 - 01:09
Min Jung Kim, Young-hye Moon, Heejoo Kim, Semi Rho, Young Kee Shin, Manki Song, Richard Walker, Cecil Czerkinsky, Dong Wook Kim, Jae-Ouk Kim
Fri, 10/26/2018 - 17:00
Cedric N. Berger, Valerie F. Crepin, Theodoros I. Roumeliotis, James C. Wright, Nicolas Serafini, Meirav Pevsner-Fischer, Lu Yu, Eran Elinav, James P. Di Santo, Jyoti S. Choudhary, Gad Frankel Infection with Citrobacter rodentium triggers robust tissue damage repair responses, manifested secretion of IL-22, in the absence of which mice succumbed to the infection. Of the main hallmarks of C....
Tue, 10/23/2018 - 20:00
Shigella spp. are Gram-negative intracellular pathogenic bacteria belonging to the family Enterobacteriaceae and can cause bacterial dysentery, a severe diarrheal disease. The pathophysiological impact of the Gra...
Fri, 10/05/2018 - 01:12
Available online 2 October 2018 Author(s): Shuaiyin Chen, Huiying Liu, Wenjuan Liang, Lijuan Hong, Bing Zhang, Lu Huang, Xiangjiao Guo, Guangcai DuanAbstractMultidrug resistances Shigella is an enormous threat of public health. The resistance genes always located in plasmids, phages and integrons, which get into bacteria cells by horizontal gene transfer (HGT). CRISPR-Cas systems are adaptive...
Tue, 09/25/2018 - 11:00
Identification of Shigella spp., Escherichia coli, and enteroinvasive E. coli (EIEC) is challenging because of their close relatedness. Distinction is vital, as infections with Shigella spp. are under surveillance of health authorities, in contrast to EIEC infections. In this study, a culture-dependent identification algorithm and a molecular identification algorithm were evaluated. Discrepancies...
Sat, 09/01/2018 - 01:11
Buyu Zhang, Longhao Ran, Mei Wu, Zezhou Li, Jiezhang Jiang, Zhen Wang, Sen Cheng, Jiaqi Fu, Xiaoyun Liu

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