Shigella

Wed, 10/23/2019 - 03:35
Little is known regarding differences in the gut microbiomes of rheumatoid arthritis (RA) patients and healthy cohorts in China. This study aimed to identify differences in the fecal microbiomes of 66 Chinese patients with RA and 60 healthy Chinese controls. The V3-V4 variable regions of bacterial 16S rRNA genes were sequenced with the Illumina system to define the bacterial composition. The...
Tue, 10/15/2019 - 03:47
Author(s): Katesaraporn Nuankeaw, Boonyanoot Chaiyosang, Thanapat Suebrasri, Somdej Kanokmedhakul, Saisaamorn Lumyong, Sophon BoonlueAbstractBioactive compounds of endophytic fungus Trichoderma polyalthiae were extracted from culture broth media. The crude extracts showed strong antimicrobial activity against human pathogens. Biologically active compounds were isolated and purified by...
Tue, 10/15/2019 - 03:34
Background In urban Australia, the burden of shigellosis is either in returning travelers from shigellosis-endemic regions or in men who have sex with men (MSM). Here, we combine genomic data with comprehensive epidemiological data on sexual exposure and travel to describe the spread of multidrug-resistant Shigella lineages. Methods A population-level study of all cultured Shigella isolates in...
Thu, 10/10/2019 - 03:29
Microcins are low-molecular-weight, ribosomally produced, highly stable, bacterial-inhibitory molecules involved in competitive, and amensalistic interactions between Enterobacteriaceae in the intestine. These interactions take place in a highly complex chemical landscape, the intestinal eco-active chemosphere, composed of chemical substances that positively or negatively influence bacterial...
Wed, 10/09/2019 - 03:40
by Jin Ling, Hong Wang, Gaomin Li, Zhen Feng, Yufei Song, Peng Wang, Hong Shao, Hu Zhou, Gang Chen Background Escherichia coli is currently unable to be reliably differentiated from Shigella species by routine matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis. In the present study, a reliable and rapid identification method was established for...
Tue, 10/08/2019 - 03:28
Dysentery is a major health threat that dramatically impacts childhood morbidity and mortality in developing countries. Various pathogenic agents cause dysentery, such as Shigella spp. and Escherichia coli, which are very closely related if not identical species. Sensitive and precise detection and identification of the infectious agent is important to target the best therapeutic strategy, but...
Sat, 09/28/2019 - 03:22
The structure and diversity of microbial communities in the leaves of Cinnamomum camphora at different growth stages were studied by high-throughput sequencing. Moreover, the relationships between microbial communities and borneol content were analyzed in this paper. The results indicated that the community structure of endophytic bacteria in C. camphora exhibited temporal variations, with the...
Tue, 09/24/2019 - 03:41
Colicin U is a protein produced by the bacterium Shigella boydii (serovars 1 and 8). It exerts antibacterial activity against strains of the enterobacterial genera Shigella and Escherichia. Here we report that colicin U forms voltage-dependent pores in planar lipid membranes; its single-pore conductance was found to be about 22 pS in 1M KCl, at pH 6 under 80 mV in asolectin bilayers. In agreement...
Sun, 09/22/2019 - 03:22
Background The ability to identify bacterial pathogens that necessitate specific clinical management or public health action in children with acute gastroenteritis is crucial to patient care and public health. However, existing stool-testing guidelines offer inconsistent recommendations, and their performance characteristics are unknown. We evaluated 6 leading gastroenteritis guidelines (eg,...
Sat, 09/21/2019 - 03:36
The gut microbiome is critical in providing resistance against colonization by exogenous microorganisms. The mechanisms via which the gut microbiota provide colonization resistance (CR) have not been fully elucidated, but they include secretion of antimicrobial products, nutrient competition, support of gut barrier integrity, and bacteriophage deployment. However, bacterial enteric infections are...
Thu, 09/19/2019 - 03:40
Author(s): Zhenghua Huang, Hailong Peng, Yong Sun, Xuemei Zhu, Hua Zhang, Li Jiang, Qiang Zhao, Hua XiongAbstractThis study aimed to evaluate the beneficial effects of novel hydrolysates produced by limited enzymatic broken rice (HEBR) on the growth performance, intestinal morphology, and gut microbiota of weaned piglets. During the enzymatic hydrolysis, the primary component of HEBR was...
Thu, 09/19/2019 - 03:37
Author(s): Yu-Lin Lee, Min-Chi Lu, Pei-Lan Shao, Po-Liang Lu, Yen-Hsu Chen, Shu-Hsing Cheng, Wen-Chien Ko, Chi-Ying Lin, Ting-Shu Wu, Muh-Yong Yen, Lih-Shinn Wang, Chang-Pan Liu, Wen-Sen Lee, Zhi-Yuan Shi, Yao-Shen Chen, Fu-Der Wang, Shu-Hui Tseng, Chao-Nan Lin, Yu-Hui Chen, Wang-Huei ShengABSTRACTMulticentre surveillance of antimicrobial susceptibility of clinically important Gram-negative...
Thu, 09/19/2019 - 03:36
Author(s): Byron Michael Berenger, Christina Ferrato, Linda ChuiAbstractTransport media are recommended to improve the sensitivity of fecal culture, but there are limited published data comparing bacterial viability in feces stored with or without transport media. In this study, recovery of bacteria from culture-positive feces after 7 days of storage was assessed under the following conditions:...
Thu, 09/19/2019 - 03:36
Author(s): Atsushi Hinenoya, Hidetoshi Ichimura, Noritomo Yasuda, Seiya Harada, Kazuhiro Yamada, Masahiro Suzuki, Yoshio Iijima, Akira Nagita, M. John Albert, Noritoshi Hatanaka, Sharda Prasad Awasthi, Shinji YamasakiAbstractMany Escherichia albertii isolates, an emerging pathogen of human and birds, might have been misidentified due to the difficulty of differentiating this bacterium from...
Thu, 09/05/2019 - 01:05
LWT
Publication date: December 2019Source: LWT, Volume 116Author(s): Saerom Lee, Misook KimAbstractHere, the multi-functional properties of Leuconostoc mesenteroides MKSR (MKSR), newly isolated from the Korean fermented vegetable product, kimchi, were evaluated. The survival rate of MKSR was 98% in 0.1% pepsin solution (pH 2.0) after 3 h, and 93% in 0.1% bile salt solution after 4 h. MKSR possessed...
Wed, 08/21/2019 - 20:00
Shigellosis is a public health threat in developed as well as developing countries like India. While antibiotic therapy is the mainstay of treatment for shigellosis, current emergence of multidrug-resistant strains of Shigella spp. has posed the problem more challenging. Lytic bacteriophages which destroy antibiotic resistant Shigella spp. have great potential in this context. In this study we...
Fri, 08/02/2019 - 17:00
Hatim F. Sati, Nienke Bruinsma, Marcelo Galas, Jenny Hsieh, Antonio Sanhueza, Pilar Ramon Pardo, Marcos A. Espinal Background Shigellosis is the second leading cause of diarrheal death globally. The global burden has been complicated the emergence of Shigella strains resistant to first line antibiotic treatments such as ciprofloxacin. This study aims to describe the epidemiologic distribution of...
Thu, 08/01/2019 - 11:00
Despite the advent of new techniques for genetic engineering of bacteria, allelic exchange through homologous recombination remains an important tool for genetic analysis. Currently, sacB-based vector systems are often used for allelic exchange, but counterselection escape, which prevents isolation of cells with the desired mutation, occasionally limits their utility. To circumvent this, we...
Tue, 07/02/2019 - 12:23
Abstract In this study, it was aimed to determinate the prevalence of extended spectrum β‐lactamases (ESBL) producing Enterobacteriaceae and genes responsible for resistance formation in cheeses that were sold in local markets in Samsun. A total of 150 cheese samples were collected from the local markets in Samsun center and were used as material. One hundred and forty‐eight ESBL positive...
Tue, 07/02/2019 - 12:16
Intracellular trafficking pathways in eukaryotic cells are essential to maintain organelle identity and structure, and to regulate cell communication with its environment. Shigella flexneri invades and subverts the human colonic epithelium by the injection of virulence factors through a type 3 secretion system (T3SS). In this work, we report the...
Thu, 06/27/2019 - 01:07
Publication date: Available online 26 June 2019 Innovative Food Science & Emerging TechnologiesAuthor(s): Hee-Jeong Hwang, Ji-Hyun Seo, Chanmin Jeong, Chan-Ick Cheigh, Myong-Soo ChungAbstractThe objective of this study was to measure the inactivation efficiencies of intense pulsed light (IPL) on six types of bacteria and determine how the efficiency values are related to the spectral...
Thu, 06/20/2019 - 11:00
Shigella flexneri is an intracellular bacterial pathogen that invades epithelial cells in the colonic mucosa, leading to bloody diarrhea. A previous study showed that S. flexneri forms biofilms in the presence of bile salts, through an unknown mechanism. Here, we investigated the potential role of adhesin-like autotransporter proteins in S. flexneri biofilm formation. BLAST search analysis...
Thu, 06/20/2019 - 01:06
LWT
Publication date: Available online 10 June 2019Source: LWTAuthor(s): Sujogya Kumar Panda, Yugal Kishore Mohanta, Laxmipriya Padhi, Walter LuytenAbstractThe anti-microbial activity of crude extracts (hexane, acetone, ethanol, and aqueous) of 46 edible plants from Odisha, India, was studied using a broth microdilution assay against 8 common food-borne pathogens (Gram-positive: Bacillus cereus...
Wed, 06/19/2019 - 17:00
Chenggong Ji, Shuo Du, Peng Li, Qinyu Zhu, Xiaoke Yang, Chunhong Long, Jin Yu, Feng Shao, Junyu Xiao The guanylate-binding proteins (GBPs) belong to the dynamin superfamily of GTPases and function in cell-autonomous defense against intracellular pathogens. IpaH9.8, an E3 ligase from the pathogenic bacterium Shigella flexneri, ubiquitinates a subset of GBPs and leads to their proteasomal...
Sun, 06/16/2019 - 01:12
Available online 14 June 2019 Author(s): Yu-Lin Lee, Min-Chi Lu, Pei-Lan Shao, Po-Liang Lu, Yen-Hsu Chen, Shu-Hsing Cheng, Wen-Chien Ko, Chi-Ying Lin, Ting-Shu Wu, Muh-Yong Yen, Lih-Shinn Wang, Chang-Pan Liu, Wen-Sen Lee, Zhi-Yuan Shi, Yao-Shen Chen, Fu-Der Wang, Shu-Hui Tseng, Chao-Nan Lin, Yu-Hui Chen, Wang-Huei ShengAbstractWe performed multicentre surveillance of the antimicrobial...
Fri, 06/14/2019 - 01:12
Available online 13 June 2019 Author(s): Khashayar Shahin, Majid Bouzari, Ran Wang, Mahsa YazdiAbstractShigella spp. can be isolated from various food sources and is responsible for many outbreaks and sporadic cases of foodborne diseases worldwide. Although Shigella species are known as one of the major foodborne pathogens, a few studies have characterized the prevalence and molecular basis of...
Thu, 06/13/2019 - 20:00
Shigella has evolved as a result of acquiring extragenetic material through horizontal gene exchange. These aid in the rapid emergence of bacterial inter-strain diversity in virulence factors and serotype variant...
Wed, 06/12/2019 - 00:00
The present study investigated whether delaying the first feeding of colostrum affected ileum and colon mucosa-associated microbiota in calves. Twenty-seven male Holstein calves were randomly assigned to 1 of 3 groups, fed colostrum at 45 min, 6 h, and 12 h after birth, respectively. Ileum and colon mucosa were collected at 51 h after birth, and their associated microbial profiles were assessed...
Thu, 06/06/2019 - 01:11
Available online 4 June 2019 Author(s): Erwin Bohn, Michael Sonnabend, Kristina Klein, Ingo B. AutenriethAbstractType III secretion systems (T3SS) play a crucial role for virulence in many Gram-negative bacteria. After tight bacterial contact to host cells, the T3SS injects effector proteins into the host cells, which leads to cell invasion, tissue destruction and/or immune evasion. Over the last...
Thu, 05/30/2019 - 11:00
An increase of Escherichia-Shigella was previously reported in acute necrotizing pancreatitis (ANP). We investigated whether Escherichia coli MG1655, an Escherichia commensal organism, increased intestinal injury and aggravated ANP in rats. ANP was induced by retrograde injection of 3.5% sodium taurocholate into the biliopancreatic duct. Using gut microbiota-depleted rats, we demonstrated that...
Tue, 05/28/2019 - 20:00
Shigella spp., facultative anaerobic bacilli of the family Enterobacteriaceae, are one of the most common causes of diarrheal diseases in human worldwide which have become a significant public health burden. So, ...
Fri, 05/24/2019 - 11:00
Although enteric multianalyte syndromic panels are increasingly employed, direct comparisons with traditional methods and the inclusion of host phenotype correlations are limited. Luminex xTAG gastrointestinal pathogen panel (GPP) and culture results are highly concordant. However, phenotypic and microbiological confirmatory testing raises concerns regarding the accuracy of the GPP, especially...
Thu, 05/16/2019 - 11:01
Microcin PDI (MccPDI), a class IIa microcin that is produced by Escherichia coli strains 25 and 284, is known to inhibit foodborne pathogenic enterohemorrhagic E. coli serotypes O157:H7 and O26. Here we demonstrate that MccPDI can inhibit Shigella strains and E. coli isolates that are multidrug resistant, the latter including strains known to cause urinary tract infections in people and companion...
Thu, 05/16/2019 - 01:08
LWT
Publication date: Available online 13 May 2019Source: LWTAuthor(s): Seyed Mohammad Bagher Hashemi, Reza Roohi, Mohammad Reza Mahmoudi, Daniel GranatoAbstractInactivation of Listeria monocytogenes (serotype 4 ab), Shigella sonnei, Byssochlamys fulva and Saccharomyces cerevisiae and the degradation of β-carotene and ascorbic acid in tangerine juice subjected to pulsed-thermosonication were modeled...
Thu, 05/16/2019 - 01:07
Available online 15 May 2019 Author(s): Narges Elahi, Mohammad Hadi Baghersad, Mehdi KamaliAbstractEarly detection of infectious bacteria is a necessity for combating infectious diseases. Due to low infectious dose of Shigella, rapid and sensitive detection is needed. Compared to the presented genes, Spa gene can be introduced as a novel sequence for all species of Shigella detection. Herein, the...
Sat, 05/11/2019 - 10:14
Abstract Aim Intrauterine growth retardation (IUGR) is a prevalent problem in mammals. The present study was conducted to unveil the alterations in intestinal microbiota in IUGR piglets. Methods and Results We identified the alterations of small intestinal microbiota in IUGR piglets on 7, 21, and 28 days of age using 16S rRNA sequencing. The results showed that IUGR piglets had a decreased...
Sun, 05/05/2019 - 01:14
Available online 2 May 2019 Author(s): Hiroki Maruoka, Atsushi Hinenoya, Noritomo Yasuda, Atsuyoshi Takeda, Shin Inoue, Tomoko Sumi, Kazuaki Koitabashi, Hiroshi Yasue, Kazuhiko Kogou, Shinji YamasakiAbstractWe developed a new GeneFields® EHEC/SS PCR dipstick DNA chromatography kit for the simultaneously detection of invA, ipaH, and stx genes in Salmonella enterica (56 strains), Shigella spp. (44...
Fri, 05/03/2019 - 01:13
Available online 30 April 2019 Author(s): R.M. Subasinghe, A.D. Samarajeewa, R. Scroggins, L.A. BeaudetteAbstractIdentification of bacteria in new or existing commercial microbial-based products (MBPs) is important for compliance with government regulations and for human and environmental risk assessment. Research was performed to develop effective methods to identify bacteria present in a MBP...
Sun, 04/28/2019 - 20:00
Shigella flexneri has an extremely complex genome with a significant number of virulence traits acquired by mobile genetic elements including bacteriophages and plasmids. S. flexneri serotype 1c is an emerging et...
Thu, 04/25/2019 - 01:12
Available online 20 April 2019 Author(s): Hang Wang, Xiaojie Qin, Si Mi, Xia Li, Xiaoling Wang, Weijiang Yan, Chunhui ZhangAbstractThe processing environment of broiler processing plants is a potential major source of bacterial contamination of broiler carcasses. This study investigated the effect of processing water and processing time on the microbial diversity of yellow-feathered broiler...
Wed, 04/24/2019 - 06:33
Scope Understanding biological functions of different free human milk oligosaccharides (HMOs) in shaping gastrointestinal tract microbiota during infancy is of great interest. We examined a link between HMOs in maternal milk and infant faecal microbiota composition and investigated the role of microbiota in degrading HMOs within the GI tract of healthy, breastfed, one‐month old infants. Methods...
Tue, 04/16/2019 - 20:00
Structural and Proteomic Changes in Viable but Non-culturable Vibrio cholerae Susanne Brenzinger1*, Lizah T. van der Aart1, Gilles P. van Wezel1, Jean-Marie Lacroix2, Timo Glatter3 and Ariane Briegel1* 1Department of Microbial Biotechnology & Health, Institute of Biology Leiden, Leiden University, Leiden, Netherlands 2Unité de Glycobiologie Structurale et Fonctionnelle, UMR CNRS 8576...
Wed, 04/10/2019 - 20:00
Campylobacter jejuni Demonstrates Conserved Proteomic and Transcriptomic Responses When Co-cultured With Human INT 407 and Caco-2 Epithelial Cells Nicholas M. Negretti1, Geremy Clair2, Prabhat K. Talukdar1, Christopher R. Gourley1, Steven Huynh3, Joshua N. Adkins2, Craig T. Parker3, Colby M. Corneau1 and Michael E. Konkel1* 1School of Molecular Biosciences, College of Veterinary Medicine,...
Tue, 04/09/2019 - 20:00
O-Antigen Gene Clusters of Plesiomonas shigelloides Serogroups and Its Application in Development of a Molecular Serotyping Scheme Daoyi Xi1,2,3,4†, Xiaochen Wang1,2,3,4†, Kexin Ning1,2,3,4, Qian Liu1,2,3,4, Fuyi Jing1,2,3,4, Xi Guo1,2,3,4 and Boyang Cao1,2,3,4* 1Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, TEDA College, Nankai University...
Tue, 04/09/2019 - 00:00
Toxins, Vol. 11, Pages 212: Roles of Shiga Toxins in Immunopathology Toxins doi: 10.3390/toxins11040212 Authors: Moo-Seung Lee Vernon L. Tesh Shigella species and Shiga toxin-producing Escherichia coli (STEC) are agents of bloody diarrhea that may progress to potentially lethal complications such as diarrhea-associated hemolytic uremic syndrome (D+HUS) and neurological disorders. The...
Mon, 04/08/2019 - 20:00
Gamma Irradiation Influences the Survival and Regrowth of Antibiotic-Resistant Bacteria and Antibiotic-Resistance Genes on Romaine Lettuce Vaishali Dharmarha1, Giselle Guron2, Renee R. Boyer1, Brendan A. Niemira3, Amy Pruden2, Laura K. Strawn1 and Monica A. Ponder1* 1Department of Food Science and Technology, Virginia Tech, Blacksburg, VA, United States 2Department of Civil and Environmental...
Mon, 04/01/2019 - 12:01
Summary Early gut microbial colonization is important for postnatal metabolic and immune development. However, little is known about the effects of different feeding modes (suckling versus bottle‐feeding) or microbial sources on this process in farm animals. We found that suckled and bottle‐fed newborn lambs had their own distinct gut microbiota. Results from 16S rRNA gene sequencing and qPCR...
Fri, 03/29/2019 - 09:54
The purpose of this study is to establish a rapid and accurate method based on multiplex polymerase chain reaction combined with suspension array xTAG (Flexible Sequence‐tagged, xTAG) technology to simultaneously detect Salmonella Typhimurium, Brucella spp., Bacillus cereus and Shigella spp. in raw milk. Summary The purpose of this study is to establish a rapid and accurate method based on...
Thu, 03/28/2019 - 11:00
Shigella spp. are a leading cause of human diarrheal disease worldwide, with Shigella flexneri being the most frequently isolated species in developing countries. This serogroup is presently classified into 19 serotypes worldwide. We report here a multicenter validation of a multiplex-PCR-based strategy previously developed by Q. Sun, R. Lan, Y. Wang, A. Zhao, et al. (J Clin Microbiol 49:3766...
Thu, 03/28/2019 - 11:00
Azithromycin (AZM) has been recommended by the American Academy of Pediatrics for the treatment of shigellosis in children. In this study, 502 Shigella species isolated between 2004 and 2014 were tested for AZM epidemiological cutoff values (ECV) by disk diffusion. AZM MICs and the presence of the macrolide resistance genes [erm(A), erm(B), erm(C), ere(A), ere(B), mph(A), mph(B), mph(D), mef(A),...

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