Shigella

Mon, 03/25/2019 - 11:00
The enteric pathogen Shigella is one of the leading causes of moderate-to-severe diarrhea and death in young children in developing countries. Transformed cell lines and animal models have been widely used to study Shigella pathogenesis. In addition to altered physiology, transformed cell lines are composed of a single cell type that does not sufficiently represent the complex multicellular...
Mon, 03/25/2019 - 11:00
The enteric bacterium and intracellular human pathogen Shigella causes hundreds of millions of cases of the diarrheal disease shigellosis per year worldwide. Shigella is acquired by ingestion of contaminated food or water; upon reaching the colon, the bacteria invade colonic epithelial cells, replicate intracellularly, spread to adjacent cells, and provoke an intense inflammatory response. There...
Fri, 03/22/2019 - 11:00
Bacteria of the genus Shigella, consisting of 4 species and >50 serotypes, cause shigellosis, a foodborne disease of significant morbidity, mortality, and economic loss worldwide. Classical Shigella identification based on selective media and serology is tedious, time-consuming, expensive, and not always accurate. A molecular diagnostic assay does not distinguish Shigella at the species level...
Sun, 03/10/2019 - 01:13
Available online 9 March 2019 Author(s): Reinhard Sting, Tobias Eisenberg, Maja HrubenjaAbstractAnalyses of short subunit gene sequences have been established for taxonomic classification and identification of bacteria and fungi. To produce partial bacterial ribosomal 16S rRNA and rpoB and fungal ribosomal ITS/LSU gene sequences for DNA sequencing, real-time PCR assays supplemented with the...
Wed, 03/06/2019 - 11:00
We developed a multiplex PCR method based on multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) that was designed for the rapid typing of Escherichia coli and Shigella isolates. The method amplifies seven VNTRs and does not require a sequencing capillary or fluorescent dyes. The amplification products are simply loaded on a standard agarose gel for electrophoresis, and the...
Thu, 02/28/2019 - 11:11
Abstract Scope : The intake of resistant starch (RS) may attenuate the risks of chronic illness, including colorectal cancer. However, whether the attenuated functionality of RS is maintained in cooked meat products needs to be clarified. Methods and Results : The effects of a cooked mixture of lean pork meat and RS (MS) on the growth performance, lipid accumulation and intestinal health of...
Tue, 02/19/2019 - 11:27
Prophage mediated horizontal gene transfer (HGT) plays a key role in the evolution of bacteria, enabling access to new environmental niches, including pathogenicity. Citrobacter rodentium is a host-adapted intestinal mouse pathogen and important model organism for attaching and effacing (A/E) pathogens including the clinically significant enterohaemorrhagic (EHEC) and enteropathogenic (EPEC)...
Sat, 02/09/2019 - 00:00
Abstract A gastroenteritis outbreak occurred in a university in May, 2017, Wuhan, China. The epidemiological survey and pathogen analysis were conducted to identify the pathogen and control this outbreak. Feces or anal swabs from individuals, water, and swabs taken from tap surfaces of the secondary water supply system (SWSS) and foods were collected for the detection of viruses...
Fri, 02/08/2019 - 17:00
James B. Thissen, Nicholas A. Be, Kevin McLoughlin, Shea Gardner, Paul G. Rack, Michael H. Shapero, Raymond R. R. Rowland, Tom Slezak, Crystal J. Jaing Microarrays have proven to be useful in rapid detection of many viruses and bacteria. Pathogen detection microarrays have been used to diagnose viral and bacterial infections in clinical samples and to evaluate the safety of biological drug...
Thu, 02/07/2019 - 01:13
Available online 6 February 2019 Author(s): Grażyna Budryn, Elżbieta Klewicka, Joanna Grzelczyk, Ilona Gałązka-Czarnecka, Radosław MostowskiAbstractLegume seeds and sprouts are a rich source of phytoestrogens in the form of isoflavonoids. For the first time, lactic acid fermentation of four types of legume sprouts was used to increase the content of isoflavonoids and microbiological safety. After...
Thu, 02/07/2019 - 01:13
Available online 31 January 2019 Author(s): S. Deshaware, S. Gupta, R. Singhal, P.S. VariyarAbstractDebittered bitter gourd juice was subjected to gamma irradiation doses of 0, 0.25, 0.5, 0.75, 1, 1.5 and 2.5 kGy and thermal pasteurization at temperatures of 65, 75, 85 and 95°C. Shigella boydii was the most heat resistant pathogen tested (D10 of 42.8 s at 65 ⁰C) while Bacillus cereus was the most...
Mon, 02/04/2019 - 01:10
Available online 31 January 2019 Author(s): Mariana Bona, Pedro Henrique Medeiros, Ana Karolina Santos, Thiago Freitas, Mara Prata, Herlice Veras, Marília Amaral, Daniel Oliveira, Alexandre Havt, Aldo Ângelo LimaABSTRACTShigella/Enteroinvasive Escherichia coli (EIEC) pathotype is a major enteropathogen associated with diarrhea and malnutrition in children from developing countries. This study...
Sun, 01/27/2019 - 01:12
Available online 25 January 2019 Author(s): Hoa Van Ba, Hyun-Woo Seo, Pil-Nam Seong, Sun-Moon Kang, Soo-Huyn Cho, Yoon-Seok Kim, Beom-Young Park, Sung-Sil Moon, Se-Ju Kang, Yong-Min Choi, Jin-Hyoung KimAbstractThis study was conducted to detect and identify microbial populations on pig carcasses at different slaughtering stages and on retail pork cuts at 24 h after slaughter as well as to...
Sat, 01/26/2019 - 01:08
Available online 22 January 2019 Author(s): Shaohua Lin, Yingyan Han, Chunzi, Yunbo Luo, Wentao Xu, Hongxia Luo, Guofang PangAbstractThe dissipation kinetics of a combined use of procymidone and thiamethoxam, and their impact on the biodiversity and pathogen on surface of tomatoes were studied. The half-lives of procymidone and thiamethoxam, used either on their own or in combination with each...
Fri, 01/25/2019 - 01:13
Publication date: Available online 23 January 2019 Innovative Food Science & Emerging TechnologiesAuthor(s): Seyed Mohammad Bagher Hashemi, Reza RoohiAbstractA comprehensive 3D, transient-free convection, and multiphase model was developed to determine the performance of ohmic heating (150, 200 and 250 V; 120 s; 99.4 °C) and conventional heating (90 °C; 15 min) for the inactivation of...
Mon, 01/14/2019 - 17:00
Jessica Guzman-Otazo, Lucia Gonzales-Siles, Violeta Poma, Johan Bengtsson-Palme, Kaisa Thorell, Carl-Fredrik Flach, Volga Iñiguez, Åsa Sjöling Water borne diarrheal pathogens might accumulate in river water and cause contamination of drinking and irrigation water. The La Paz River basin, including the Choqueyapu River, flows through La Paz city in Bolivia where it is receiving sewage, and...
Fri, 12/14/2018 - 01:11
Chaojie Yang, Haiying Wang, Hui Ma, Renlong Bao, Hongbo Liu, Lang Yang, Beibei Liang, Leili Jia, Jing Xie, Ying Xiang, Nian Dong, Shaofu Qiu, Hongbin Song
Sat, 12/08/2018 - 01:11
Available online 6 December 2018 Author(s): Sinosh Skariyachan, Sanjana GovindarajanAbstractThe present study elucidates biopreservation potential of an antimicrobial protein; bacteriocin, producing Pediococcus spp. isolated from dairy sample and enhancement of their shelf life in comparison with two chemical preservatives. The antimicrobial protein producing Pediococcus spp. was isolated from...
Fri, 12/07/2018 - 17:00
Valeria Ciancarella, Luigi Lembo-Fazio, Ida Paciello, Anna-Karin Bruno, Sébastien Jaillon, Sara Berardi, Marialuisa Barbagallo, Shiri Meron-Sudai, Dani Cohen, Antonio Molinaro, Giacomo Rossi, Cecilia Garlanda, Maria Lina Bernardini Shigella spp. are pathogenic bacteria that cause bacillary dysentery in humans invading the colonic and rectal mucosa where they induce dramatic inflammation. Here,...
Fri, 12/07/2018 - 01:10
Publication date: February 2019 , Volume 148Author(s): Daphney Shiningeni, Percy Chimwamurombe, Renatus Shilangale, Jane MisihairabgwiAbstractTo determine the prevalence of pathogenic bacteria in street vended ready-to-eat meats in Windhoek, Namibia, a total of 96 street vended ready to eat meat samples were evaluated. Prevalences of 42%, 52%, 15%, 6% and 83% were observed for Escherichia coli,...
Thu, 12/06/2018 - 01:10
Publication date: October 2018 , Volume 308, Issue 7Author(s): Michael Berger, Petya Berger, Erick Denamur, Alexander Mellmann, Ulrich DobrindtAbstractThe highly virulent Escherichia coli O104:H4 isolate that caused a large outbreak in 2011 carries three plasmids. Out of these, only one, the IncI plasmid pO104_90 that encodes two extended spectrum beta-lactamases, can transfer itself by...
Wed, 11/28/2018 - 01:10
Available online 27 November 2018 Author(s): Junli Feng, Qing Shen, Jiajia Wu, Zhiyuan Dai, Yi WangAbstractShigella flexneri is one of the major food-borne pathogens in China. Herein, we reported the development of a colorimetric aptasensor based on aptamer and gold nanoparticles (AuNPs) for detection of Sh. flexneri. Aptamer with high binding affinity to Sh. flexneri was firstly immobilized on...
Thu, 11/01/2018 - 01:09
Min Jung Kim, Young-hye Moon, Heejoo Kim, Semi Rho, Young Kee Shin, Manki Song, Richard Walker, Cecil Czerkinsky, Dong Wook Kim, Jae-Ouk Kim
Fri, 10/26/2018 - 17:00
Cedric N. Berger, Valerie F. Crepin, Theodoros I. Roumeliotis, James C. Wright, Nicolas Serafini, Meirav Pevsner-Fischer, Lu Yu, Eran Elinav, James P. Di Santo, Jyoti S. Choudhary, Gad Frankel Infection with Citrobacter rodentium triggers robust tissue damage repair responses, manifested secretion of IL-22, in the absence of which mice succumbed to the infection. Of the main hallmarks of C....
Tue, 10/23/2018 - 20:00
Shigella spp. are Gram-negative intracellular pathogenic bacteria belonging to the family Enterobacteriaceae and can cause bacterial dysentery, a severe diarrheal disease. The pathophysiological impact of the Gra...
Fri, 10/05/2018 - 01:12
Available online 2 October 2018 Author(s): Shuaiyin Chen, Huiying Liu, Wenjuan Liang, Lijuan Hong, Bing Zhang, Lu Huang, Xiangjiao Guo, Guangcai DuanAbstractMultidrug resistances Shigella is an enormous threat of public health. The resistance genes always located in plasmids, phages and integrons, which get into bacteria cells by horizontal gene transfer (HGT). CRISPR-Cas systems are adaptive...
Tue, 09/25/2018 - 11:00
Identification of Shigella spp., Escherichia coli, and enteroinvasive E. coli (EIEC) is challenging because of their close relatedness. Distinction is vital, as infections with Shigella spp. are under surveillance of health authorities, in contrast to EIEC infections. In this study, a culture-dependent identification algorithm and a molecular identification algorithm were evaluated. Discrepancies...
Sat, 09/01/2018 - 01:11
Buyu Zhang, Longhao Ran, Mei Wu, Zezhou Li, Jiezhang Jiang, Zhen Wang, Sen Cheng, Jiaqi Fu, Xiaoyun Liu
Thu, 07/26/2018 - 00:00
This study was carried out to assess the sanitary quality of processed (fried) freshwater clam (Galatea paradoxa), sold in Yenagoa metropolis, Bayelsa State, Nigeria. “Water snail” as it is popularly called is vended by women and children. This delicacy is widely consumed in Yenagoa and its environs. Sixteen (16) Samples were collected randomly from four different hawkers within the Swali market...
Mon, 07/23/2018 - 11:00
Shigella flexneri disseminates within the colonic mucosa by displaying actin-based motility in the cytosol of epithelial cells. Motile bacteria form membrane protrusions that project into adjacent cells and resolve into double-membrane vacuoles (DMVs) from which the bacteria escape, thereby achieving cell-to-cell spread. During dissemination, S. flexneri is targeted by LC3-dependent autophagy, a...
Tue, 07/10/2018 - 01:12
Available online 9 July 2018 Author(s): Jiamu Kang, Liu Liu, Meihui Liu, Xiaoxia Wu, Jianke LiAbstractPersistent bacterial infections caused by biofilm- associated Shigella spp. are a global public health concern. The following study aims to determine the antibacterial activity and possible mechanism that gallic acid (GA) displays against Shigella flexneri (Sh. flexneri) and its effect on the...
Fri, 07/06/2018 - 01:14
Publication date: July 2018 , Volume 52, Issue 1Author(s): Qiuxia Ma, Yong Huang, Jian Wang, Xuebin Xu, Jane Hawkey, Chaojie Yang, Beibei Liang, Xiaofeng Hu, Fuli Wu, Xiaoxia Yang, Jinyan Wang, Rongrui Li, Peng Li, Jing Xie, Leili Jia, Ligui Wang, Rongzhang Hao, Yigang Tong, Kathryn E. Holt, Shaofu QiuAbstractSince the plasmid-mediated polymyxin resistance gene mcr-1 was first reported in...
Fri, 07/06/2018 - 01:14
Publication date: June 2018 , Volume 51, Issue 6Author(s): Delfino Vubil, Clara Balleste-Delpierre, Rita Mabunda, Sozinho Acácio, Marcelino Garrine, Tacilta Nhampossa, Pedro Alonso, Inacio Mandomando, Jordi VilaAbstractThe objective of this study was to assess antibiotic resistance and the molecular epidemiology of shigella isolates from a case–control study of diarrhoea, conducted from 2007 to...
Mon, 07/02/2018 - 01:08
Xiaopan Gao, Zhixia Mu, Xia Yu, Bo Qin, Justyna Wojdyla, Meitian Wang, Sheng Cui
Tue, 06/19/2018 - 12:04
Publication date: July 2018 , Volume 101, Issue 7 Author(s): Su Chen, Lin Chen, Lie Chen, Xueliang Ren, Hongjuan Ge, Baolei Li, Guanghui Ma, Xueqin Ke, Jun Zhu, Li Li, Yuhong Feng, Yanjun Li The aim of this study was to select probiotic strains that could be used in drinkable yogurt to yield viable cells following storage at room temperature (RT). The uniquely high...
Tue, 06/05/2018 - 17:00
Tryntsje Cuperus, Marina D. Kraaij, Aldert L. Zomer, Albert van Dijk, Henk P. Haagsman Host Defense Peptides (HDPs) such as cathelicidins are multifunctional effectors of the innate immune system with both antimicrobial and pleiotropic immunomodulatory functions. Chicken cathelicidin-2 (CATH-2) has multiple immunomodulatory effects in vitro and the D-amino acid analog of this peptide has been...
Tue, 05/29/2018 - 20:27
Publication date: October 2018 , Volume 92 Author(s): Chak-Lun Chan, Ren-You Gan, Nagendra P. Shah, Harold Corke Phenolic rich extracts from edible plants exhibit antimicrobial ability against various pathogens, however, their effects on probiotic bacteria have been much less investigated. This study, therefore, investigated and compared the effects of phenolic rich extracts from six...
Sun, 05/20/2018 - 03:43
Publication date: July 2018 , Volume 109 Author(s): Madeshwari Ezhilan, Noel Nesakumar, K. Jayanth Babu, C.S. Srinandan, John Bosco Balaguru Rayappan Foodborne pathogens cause serious health issues and have a strong impact on the economy of the country. In this context, quality testing of royal delicious apple by detecting pathogen contamination using an electronic nose, which...
Sat, 05/19/2018 - 10:53
Publication date: 2 August 2018 , Volume 278 Author(s): Mohammad Aminul Islam, Subarna Roy, Ashikun Nabi, Sultana Solaiman, Mahdia Rahman, Mohsina Huq, Nurul Amin Siddiquee, Niyaz Ahmed The main objective of the study was to assess the microbiological quality of milk at different stages of the dairy value chain from farm to the factory in Bangladesh. A total of 438 raw milk...
Sun, 05/13/2018 - 20:00
ABSTRACTThe present study was conducted to estimate the apparent prevalence of Salmonella spp. in free-ranging waterfowl that inhabitant Entre Ríos, Argentina, determine the antimicrobial resistance of the isolated, and compare the performance of two selective plating media used for Salmonella isolation. Five hundred ninety nine free-living waterfowl were sampled one time by cloacal swab from...
Thu, 05/03/2018 - 10:24
Publication date: Available online 2 May 2018 Author(s): Adriana Calderaro, Monica Martinelli, Mirko Buttrini, Sara Montecchini, Silvia Covan, Sabina Rossi, Francesca Ferraglia, Paolo Montagna, Federica Pinardi, Sandra Larini, Maria Cristina Arcangeletti, Maria Cristina Medici, Carlo Chezzi, Flora De Conto This study represents a 2-year picture of the epidemiology...
Mon, 04/16/2018 - 11:00
Shigella flexneri and Shigella sonnei bacteria cause the majority of all shigellosis cases worldwide. However, their distributions differ, with S. sonnei predominating in middle- and high-income countries and S. flexneri predominating in low-income countries. One proposed explanation for the continued range expansion of S. sonnei is that it can survive in amoebae, which could provide a protective...
Tue, 04/03/2018 - 17:00
Hongbo Liu, Binghua Zhu, Shaofu Qiu, Yidan Xia, Beibei Liang, Chaojie Yang, Nian Dong, Yongrui Li, Ying Xiang, Shan Wang, Jing Xie, Muti Mahe, Yansong Sun, Hongbin Song Shigella represents one of the major diarrhea-inducing pathogens threatening public health, but its prevalence and antimicrobial resistance profile in Xinjiang Uygur Autonomous region, China, remains unclear. We conducted...
Mon, 04/02/2018 - 11:00
Since the initial discovery of mcr-1 in an Escherichia coli isolate from China, the gene has also been detected in Klebsiella pneumoniae and Salmonella enterica but is rarely reported in other Enterobacteriaceae. Here, we report the isolation and identification of a Shigella flexneri strain harboring mcr-1 from stool samples in a pig farm in China from 2009. The MIC of colistin for the isolate is...
Mon, 04/02/2018 - 11:00
Symbiotic microorganisms have been found in the hemolymph (blood) of many aquatic invertebrates, such as crabs, shrimp, and oysters. Hemolymph is a critical site in the host immune response. Currently, studies on hemolymph microorganisms are mostly performed with culture-dependent strategies using selective media (e.g., thiosulfate-citrate-bile salts-sucrose [TCBS], 2216E, and LB) for enumerating...
Thu, 03/22/2018 - 11:01
The enteric attaching and effacing (A/E) pathogens enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC) and the invasive pathogens enteroinvasive E. coli (EIEC) and Shigella encode type III secretion systems (T3SS) used to inject effector proteins into human host cells during infection. Among these are a group of effectors required for NF-B-mediated host immune evasion....
Sun, 12/17/2017 - 13:41
Publication date: Available online 17 December 2017 Author(s): Xi-Jun Tang, Ze Yang, Xin-Bin Chen, Wen-Fang Tian, Cheng-Ning Tu, Hai-Bo Wang Salmonella spp./Shigella spp. are often associated with food poisoning and fecal-oral transmission of acute gastroenteritis that requires strict monitoring, especially among people who would handle food and water. In 2014, the National...

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