Shigella

Tue, 02/21/2017 - 17:00
Milica Milivojevic, Anne-Sophie Dangeard, Christoph Alexander Kasper, Therese Tschon, Mario Emmenlauer, Claudine Pique, Pamela Schnupf, Julie Guignot, Cécile Arrieumerlou During infection invasive bacteria, epithelial cells contribute to innate immunity via the local secretion of inflammatory cytokines. These are directly produced infected cells or uninfected bystanders via connexin-dependent...
Mon, 02/13/2017 - 17:00
Francesca Necchi, Allan Saul, Simona Rondini Serum Bactericidal Activity (SBA) assay is the method of choice to evaluate the complement-mediated functional activity of both infection- and vaccine-induced antibodies. To perform a typical SBA assay, serial dilutions of sera are incubated with target bacterial strains and complement. The conventional SBA assay is based on plating on agar the SBA...
Wed, 01/25/2017 - 11:00
Escherichia coli and Shigella species are closely related and genetically constitute the same species. Differentiating between these two pathogens and accurately identifying the four species of Shigella are therefore challenging. The organism-specific bioinformatics whole-genome sequencing (WGS) typing pipelines at Public Health England are dependent on the initial identification of the bacterial...
Wed, 01/18/2017 - 06:50
Abstract The behavior of thirteen foodborne bacteria onto whole Hass avocado and the antibacterial effect of Hibiscus sabdariffa calyx extracts, sodium hypochlorite, acetic acid, and colloidal silver against foodborne bacteria on contaminated avocado were investigated. Avocados were inoculated with foodborne bacteria: Listeria monocytogenes, Shigella flexneri, Staphylococcus aureus, Salmonella...
Thu, 12/22/2016 - 02:45
Abstract AimsExplorations of freshwater cyanobacteria as antimicrobial (bacteria, fungi and MRSA strains) drug resource using bioassay, NRPS (non-ribosomal polypeptide synthetase) and PKS (polyketide synthase) genes, as well as in silico approach. Methods and ResultsWe have bioassayed the extracts of Phormidium CCC727, Geitlerinema CCC728, Arthrospira CCC729, Leptolyngbya CCC732, Phormidium...
Thu, 11/24/2016 - 04:25
Summary Although ionising radiation has been shown to kill human pathogens Shigella spp. and Aeromonas spp. on various food products, there is lack of information regarding the relative efficacy of gamma radiation against their free-living planktonic and biofilm-associated cells. The radiation sensitivity (D10 values) of planktonic, glass- and carrot-associated biofilm cells of Shigella spp. and...
Tue, 11/22/2016 - 01:19
Mohammad Uzzal Hossain, Md. Arif Khan, Abu Hashem, Md. Monirul Islam, Mohammad Neaz Morshed, Chaman Ara Keya, Md. Salimullah
Thu, 11/17/2016 - 07:07
LWT
Publication date: April 2017 Source:LWT - Food Science and Technology, Volume 77 Author(s): M.E. Sotelo-Boyás, Z.N. Correa-Pacheco, S. Bautista-Baños, M.L. Corona-Rangel Lime oil has recognized fungicidal and antibacterial properties. Nanoparticles and nanocapsules are structures that have been developed to overcome the high volatility of essential oils. Chitosan nanoparticles and...
Thu, 11/17/2016 - 02:33
Abstract AimsTo evaluate the comparative immunogenic potential of food grade Lactococcus lactis (L. lactis) expressing Outer membrane protein A (OmpA) of Shigella dysenteriae type-1 (SD-1) when administered either orally or intranasally. Methods and ResultsOmpA of SD-1 was cloned and expressed first in Escherichia coli (E. coli) and then in L. lactis. Presence of recombinant gene was confirmed by...
Thu, 11/03/2016 - 17:00
Huajun Zheng, Hong Liang, Yuezhu Wang, Maohua Miao, Tao Shi, Fen Yang, Enuo Liu, Wei Yuan, Zai-Si Ji, De-Kun Li Eczema is frequently the first manifestation of an atopic diathesis and alteration in the diversity of gut microbiota has been reported in infants with eczema. To identify specific bacterial communities associated with eczema, we conducted a case-control study of 50 infants with eczema...
Mon, 10/31/2016 - 00:00
<span class="paragraphSection"><div class="boxTitle">Abstract</div><span style="font-style:italic;">Shigella sonnei</span> and <span style="font-style:italic;">Salmonella</span> Typhi cause significant morbidity and mortality. We exploited the safety record of the oral, attenuated <span style="font-style:italic;">S.</span> Typhi vaccine (Ty21a...
Thu, 10/27/2016 - 06:21
Summary This study aimed to study the antimicrobial effect of propolis and determine the essential compounds which give it the antimicrobial activity. The antimicrobial effect was investigated for six ethyl acetate extracts of propolis (EAPs) collected from different regions of Algeria on four pathogenic strains (Shigella dysenteriae, Shigella sonnei, Staphylococcus aureus and Escherichia coli)...
Wed, 10/26/2016 - 09:00
Abstract Shigellosis represents a major burden of disease in developing countries. A low infectious dose allows the disease to be spread effectively. Although shigellosis is mostly a self-limiting disease, antibiotics are recommended to reduce deaths, disease symptoms, and organism-shedding time. However, In India, antimicrobial resistance among the genus Shigella is more common than among any...
Sat, 10/15/2016 - 05:05
Publication date: December 2016 , Volume 131 Author(s): Maaike J.C. van den Beld, Alexander W. Friedrich, Evert van Zanten, Frans A.G. Reubsaet, Mirjam A.M.D. Kooistra-Smid, John W.A. Rossen An inter-laboratory collaborative trial for the evaluation of diagnostics for detection and identification of Shigella species and Entero-invasive Escherichia coli (EIEC) was performed....
Mon, 10/10/2016 - 00:00
We developed a multiplex PCR-based procedure followed by high-performance liquid chromatography (mPCR-HPLC) assay for high-throughput screening foodborne pathogens, including Salmonella spp., Listeria monocytogenes, Enterobacter sakazakii, Staphylococcus aureus, Shigella spp., Escherichia coli O157:H7, Vibrio parahaemolyticus, Vibrio cholerae, and Vibrio vulnificus. Vibrio...
Thu, 10/06/2016 - 01:18
Li Wang, Lei Liu, Dong Liu, Zhe Yin, Jiao Feng, Defu Zhang, Haihong Fang, Yefeng Qiu, Weijun Chen, Ruisheng Yang, Jinglin Wang, Yunzhi Fa, Dongsheng Zhou
Tue, 09/27/2016 - 17:00
Madushini N. Dharmasena, Catherine M. Feuille, Carly Elizabeth C. Starke, Arvind A. Bhagwat, Scott Stibitz, Dennis J. Kopecko The licensed oral, live-attenuated bacterial vaccine for typhoid fever, Salmonella enterica serovar Typhi strain Ty21a, has also been utilized as a vaccine delivery platform for expression of diverse foreign antigens that stimulate protection against shigellosis, anthrax...
Thu, 09/08/2016 - 17:00
Petra Zadravec, Lucie Marečková, Hana Petroková, Vesna Hodnik, Milica Perišić Nanut, Gregor Anderluh, Borut Štrukelj, Petr Malý, Aleš Berlec Infections with shiga toxin-producing bacteria, like enterohemorrhagic Escherichia coli and Shigella dysenteriae, represent a serious medical problem. No specific and effective treatment is available for patients with these infections, creating a need for...
Wed, 09/07/2016 - 17:00
Xuehan Zhang, Meng Li, Bicheng Zhang, Kangming Chen, Kongwang He Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a zoonotic pathogen of worldwide importance that causes foodborne infections in humans. Intimin gamma 1 (intimin γ1) is one of the most important outer membrane proteins required for EHEC’s intimate adhesion to epithelial cells. Here, we generated a polyclonal antibody (pAb) and...
Thu, 06/16/2016 - 14:01
Publication date: August 2016 , Volume 127 Author(s): Nadim Ahamad, Dhirendra S. Katti Bacterial lipopolysaccharide (LPS) has been widely used as an antigen and adjuvant in immunological applications. Amongst the methods developed for extraction of LPS, hot phenol extraction (HPE) method is the gold standard. However, the HPE method provides poor yield of LPS (~4.5% by weight), is...
Tue, 06/07/2016 - 05:52
Shigella dysenteriae and Shigella flexneri are the causative agents of gastric infections in human. These bacteria have been known as a causative agent of food poisoning in human. The present study was carried out in order to isolate Shigella species and detect S. dysenteriae and S. flexneri in meat and meat product samples presented in Isfahan province, as well as to study the antibiotic...
Tue, 06/07/2016 - 01:40
Abstract This study was aimed at developing a one-tube multiplex real-time PCR system for simultaneous detection of five major foodborne pathogens (Staphylococcus aureus, Listeria monocytogenes, Salmonella enterica, Vibrio parahaemolyticus and Shigella spp.) in food samples. The newly developed multiplex real-time PCR was performed using fluorescent dye EvaGreen and melting curve analysis for...
Mon, 05/23/2016 - 11:01
Shigella flexneri can be phenotypically serotyped using antisera raised to type-specific somatic antigens and group factor antigens and genotypically serotyped using PCR targeting O-antigen synthesis or modification genes. The aim of this study was to evaluate a real-time PCR for serotyping S. flexneri and to use whole-genome sequencing (WGS) to investigate the phenotypic and genotypic serotype...
Thu, 05/19/2016 - 17:00
Shijun Li, Jianping Wang, Xiaoyu Wei, Ying Liu, Lu You, Xia Luo, Guangpeng Tang, Qiangzheng Sun, Changyun Ye, Jianguo Xu, Dingming Wang Background Shigellosis is a serious problem in Guizhou and Shigella sonnei is an increasingly prevalent etiologic agent of local shigellosis cases. No data, however, are available about the molecular characterization of the local isolates of S. sonnei. We have...
Mon, 05/16/2016 - 17:00
Allon Weiner, Nora Mellouk, Noelia Lopez-Montero, Yuen-Yan Chang, Célia Souque, Christine Schmitt, Jost Enninga Intracellular pathogens include all viruses, many bacteria and parasites capable of invading and surviving within host cells. Key to survival is the subversion of host cell pathways the pathogen for the purpose of propagation and evading the immune system. The intracellular bacterium...
Mon, 05/16/2016 - 11:00
Most Escherichia coli strains are nonpathogenic. However, for clinical diagnosis and food safety analysis, current identification methods for pathogenic E. coli either are time-consuming and/or provide limited information. Here, we utilized a custom DNA microarray with informative genetic features extracted from 368 sequence sets for rapid and high-throughput pathogen identification. The FDA...
Tue, 04/26/2016 - 13:02
Upon entry into host cells, intracellular bacterial pathogens establish a variety of replicative niches. Although some remodel phagosomes, others rapidly escape into the cytosol of infected cells. Little is currently known regarding how professional intracytoplasmic pathogens, including Shigella, mediate phagosomal escape. Shigella, like many other Gram-negative bacterial pathogens, uses a...
Mon, 04/18/2016 - 11:01
Bacterial type III secretion system (T3SS) effector proteins are critical determinants of infection for many animal and plant pathogens. However, monitoring of the translocation and delivery of these important virulence determinants has proved to be technically challenging. Here, we used a genetically engineered LOV (light-oxygen-voltage) sensing domain derivative to monitor the expression,...
Sat, 04/02/2016 - 14:39
Summary AraC Negative Regulators (ANR) suppress virulence genes by directly down-regulating AraC/XylS members in Gram-negative bacteria. In this study we sought to investigate the distribution and molecular mechanisms of regulatory function for ANRs among different bacterial pathogens. We identified more than 200 ANRs distributed in diverse clinically important gram negative pathogens, including...
Fri, 03/25/2016 - 11:01
Enterotoxigenic Escherichia coli (ETEC) and Shiga toxin-producing E. coli (STEC) are important causes of diarrhea and edema disease in swine. The majority of swine-pathogenic E. coli strains belong to a limited range of O serogroups, including O8, O138, O139, O141, O147, O149, and O157, which are the most frequently reported strains worldwide. However, the circumstances of ETEC and STEC...
Fri, 03/25/2016 - 11:01
Diarrheal disease is a complex syndrome that remains a leading cause of global childhood morbidity and mortality. The diagnosis of enteric pathogens in a timely and precise manner is important for making treatment decisions and informing public health policy, but accurate diagnosis is a major challenge in industrializing countries. Multiplex molecular diagnostic techniques may represent a...
Thu, 03/24/2016 - 07:07
LWT
Publication date: September 2016 Source:LWT - Food Science and Technology, Volume 71 Author(s): Roya Moghimi, Atousa Aliahmadi, David Julian McClements, Hasan Rafati There is growing interest in the development of natural antimicrobials to preserve foods. In this research, Sage (Salvia officinalis) essential oil was converted into a nanoemulsion using nonionic surfactants (Tween 80,...
Thu, 03/24/2016 - 00:57
Publication date: September 2016 , Volume 67 Author(s): Nelly Denis, Helen Zhang, Alexandre Leroux, Roger Trudel, Henri Bietlot In recent years, fresh fruits and vegetables have been linked to numerous foodborne illness outbreaks in different regions of the world, including in Canada. In light of rising concerns over the microbial safety of these commodities, the Canadian Food...
Thu, 03/17/2016 - 00:00
Shiga toxins (Stxs) produced by Shiga toxin-producing bacteria Shigella dysenteriae serotype 1 and select serotypes of Escherichia coli are primary virulence factors in the pathogenesis of hemorrhagic colitis progressing to potentially fatal systemic complications, such as hemolytic uremic syndrome and central nervous system abnormalities. Current therapeutic options to treat patients infected...
Sun, 03/06/2016 - 06:48
Abstract In this study, we comprehensively investigated the effect of dietary protein sources on the gut microbiome of weaned piglets with diets comprising different protein source using High-throughput 16SrRNA gene-based Illumina Miseq. A total of 48 healthy weaned piglets were allocated randomly to four treatments with 12 piglets in each group. The weaned piglets were fed with diets containing...
Thu, 02/25/2016 - 17:00
Yong Xue, Jon G. Wilkes, Ted J. Moskal, Anna J. Williams, Willie M. Cooper, Rajesh Nayak, Fatemeh Rafii, Dan A. Buzatu Standard methods to detect Escherichia coli contamination in food use the polymerase chain reaction (PCR) and agar culture plates. These methods require multiple incubation steps and take a long time to results. An improved rapid flow-cytometry based detection method was...
Fri, 02/19/2016 - 11:01
Pathogen identification and microbial source tracking (MST) to identify sources of fecal pollution improve evaluation of water quality. They contribute to improved assessment of human health risks and remediation of pollution sources. An MST microarray was used to simultaneously detect genes for multiple pathogens and indicators of fecal pollution in freshwater, marine water, sewage-contaminated...
Fri, 01/22/2016 - 03:19
Publication date: Available online 21 January 2016 Author(s): Meiling Jiang, Fen Zhang, Cuixiang Wan, Yonghua Xiong, Nagendra P. Shah, Hua Wei, Xueying Tao Lactobacillus plantarum WLPL04, a specific strain isolated from human breast milk, was investigated for its survival capacity (acid and bile salt tolerance, survival in simulated gastrointestinal tract, inhibition of...
Wed, 01/20/2016 - 23:47
Abstract This study aimed to investigate the antimicrobial activities of organic acids, carvacrol and their combination against Shigella sonnei. Shigella sonnei at a density of approximately 7.4 log cfu/mL in tryptic soy broth (TSB) was barely inactivated by 0.5% w/v acetic or citric acid; however, the number of S. sonnei were reduced to 4.53 and 3.25 log cfu/mL by 0.5% w/v malic and lactic acid...

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