Shigella

Tue, 01/05/2016 - 09:35
Publication date: June 2016 , Volume 64 Author(s): Yi-Gang Xu, Zhong-Mei Liu, Bai-Qi Zhang, Min Qu, Chun-Sheng Mo, Jia Luo, Su-Long Li In the present study, a novel target-enriched multiplex PCR (Tem-PCR) assay was developed for simultaneous detection of Salmonella spp., Listeria (L.) monocytogenes, Staphylococcus (S.) aureus, Escherichia (E.) coli O157:H7 and Shigella spp.. DNA...
Mon, 01/04/2016 - 11:53
The periplasmic chaperone SurA is critical for the biogenesis of outer membrane proteins (OMPs) and thus the maintenance of membrane integrity in Escherichia coli. The activity of this modular chaperone has been attributed to a core chaperone module, with only minor importance assigned to the two SurA peptidyl-prolyl isomerase (PPIase) domains. In this work, we used synthetic phenotypes and...
Fri, 01/01/2016 - 21:18
Publication date: Available online 23 December 2015 Author(s): Luana Maggiore, Lu Yu, Ulrich Omasits, Omar Rossi, Gordon Dougan, Nicholas R. Thomson, Allan Saul, Jyoti S. Choudhary, Christiane Gerke Outer membrane blebs are naturally shed by Gram-negative bacteria and are candidates of interest for vaccines development. Genetic modification of bacteria to induce...
Thu, 12/03/2015 - 17:00
Sara M. Pires, Christa L. Fischer-Walker, Claudio F. Lanata, Brecht Devleesschauwer, Aron J. Hall, Martyn D. Kirk, Ana S. R. Duarte, Robert E. Black, Frederick J. Angulo Background Diarrhoeal diseases are major contributors to the global burden of disease, particularly in children. However, comprehensive estimates of the incidence and mortality due to specific aetiologies of diarrhoeal diseases...
Thu, 11/05/2015 - 02:52
Summary Flagellar Type III secretion systems (T3SS) contain an essential Cytoplasmic-ring (C-ring) largely composed of two proteins FliM and FliN, whilst an analogous substructure for the closely related non-flagellar (NF) T3SS has not been observed in situ. We show that the spa33 gene encoding the putative NF-T3SS C-ring component in Shigella flexneri is alternatively translated to produce both...
Sat, 10/31/2015 - 00:57
Abstract The aims of this investigation were to; a) study the effect of X-ray treatments in reducing E. coli O157:H7, L. monocytogenes, S. enterica and S. flexneri on whole mangoes, and b) study the effect of X-ray treatments on microflora counts (mesophilic counts, psychrotrophic counts and yeast and mold counts) of whole mangoes during storage at ambient temperature (22 °C) for 30 days. A...
Tue, 10/20/2015 - 06:59
Summary Shigella sonnei is a bacterial pathogen and causative agent of bacillary dysentery. It deploys a type III secretion system to inject effector proteins into host epithelial cells and macrophages, an essential step for tissue invasion and immune evasion. Although the arsenal of bacterial effectors and their cellular targets have been studied extensively, little is known about the...
Mon, 10/19/2015 - 22:36
Abstract AimsProbiotics have been proved to be the most preferred and effective alternative to antibiotics as growth promoter and pathogens inhibitor in poultry industry. In this study Bacillus subtilis CGMCC 1.1086 as a probiotic bacterium was administered in diet and its effects on both the growth performance and the cecal microbiota of broilers were evaluated. Methods and ResultsA total of 240...
Fri, 10/16/2015 - 17:00
Richard Lu, BobBrooke Herrera, Heather D. Eshleman, Yang Fu, Alexander Bloom, Zhigang Li, David B. Sacks, Marcia B. Goldberg The intracellular bacterial pathogen Shigella infects and spreads through the human intestinal epithelium. Effector proteins delivered Shigella into cells promote infection modulating diverse host functions. We demonstrate that the effector protein OspB interacts directly...
Fri, 09/04/2015 - 01:23
Publication date: Available online 3 September 2015 Author(s): Chen Zhang, Peihua Niu, Yanying Hong, Ji Wang, Jingyun Zhang, Xuejun Ma Objectives We aim to develop a multiplex real-time PCR assay to detect the most common pathogens causing community outbreaks of diarrhea. Methods Four reaction systems of fluorescence dye-based real-time PCR assay were performed to amplify genes...
Sun, 08/23/2015 - 06:20
LWT
Publication date: January 2016 Source:LWT - Food Science and Technology, Volume 65 Author(s): Ji-Yeon Lee, Dong-Hyun Kang We developed an improved selective medium (HEX medium, modified Hektoen Enteric agar medium) for the detection of Shigella spp. from foods. Conventional culture media for Shigella spp. are neither specific nor sensitive. HEX medium contains lactose, sucrose, d -xylose...
Sat, 08/08/2015 - 01:19
Diarrhea causes significant morbidity and mortality in Indian children under 5 years of age. Flies carry enteric pathogens and may mediate foodborne infections. In this study, we characterized fly densities as a determinant of infectious diarrhea in a longitudinal cohort of 160 urban and 80 rural households with 1,274 individuals (27% under 5 years of age) in Vellore, India. Household...
Thu, 08/06/2015 - 01:22
Publication date: Available online 5 August 2015 Author(s): Kan Deng, Tingtao Chen, Qinglong Wu, Hongbo Xin, Qiang Wei, Ping Hu, Xiaolei Wang, Xin Wang, Hua Wei, Nagendra P. Shah Very limited information exists on the exclusion of pathogens by probiotics in the gut of the host challenged with pathogens. In this study, we tested probiotic characteristics in vitro and...
Tue, 08/04/2015 - 01:26
Publication date: 2 December 2015 , Volume 214 Author(s): Sigrun J. Hauge, Truls Nesbakken, Birgitte Moen, Ole-Johan Røtterud, Sissel Dommersnes, Ole Nesteng, Øyvin Østensvik, Ole Alvseike This study investigated the bacterial dynamics along the beef chain for clean and dirty cattle in the slaughter and processing lines, using classic quantitative methods and molecular...
Fri, 07/17/2015 - 03:20
Publication date: June–August 2015 , Volume 305, Issues 4–5 Author(s): Angela Lluque, Susan Mosquito, Cláudia Gomes, Maribel Riveros, David Durand, Drake H. Tilley, María Bernal, Ana Prada, Theresa J. Ochoa, Joaquim Ruiz The study was aimed to describe the serotype, mechanisms of antimicrobial resistance, and virulence determinants in Shigella spp. isolated from Peruvian...
Fri, 07/17/2015 - 03:20
Publication date: June–August 2015 , Volume 305, Issues 4–5 Author(s): Armand Paauw, Debby Jonker, Guus Roeselers, Jonathan M.E. Heng, Roos H. Mars-Groenendijk, Hein Trip, E. Margo Molhoek, Hugo-Jan Jansen, Jan van der Plas, Ad L. de Jong, Joanna A. Majchrzykiewicz-Koehorst, Arjen G.C.L. Speksnijder E. coli – Shigella species are a cryptic group of bacteria in which...
Fri, 07/17/2015 - 01:19
Publication date: January 2016 , Volume 59 Author(s): Chunmei Song , Cheng Liu , Shuyan Wu , Haolin Li , Huiqin Guo , Biao Yang , Shi Qiu , Jianwu Li , Lan Liu , Haijuan Zeng , Xuzhao Zhai , Qing Liu A colloidal gold immunochromatographic strip (ICS) was proposed with double monoclonal antibodies (McAbs) for rapid detections of multiple foodborne pathogens, and we successfully...
Fri, 07/17/2015 - 01:19
Publication date: January 2016 , Volume 59 Author(s): Min-Jeong Kim , Marta Mikš-Krajnik , Amit Kumar , Hyun-Gyun Yuk The objective of this study was to evaluate the antibacterial effect of 405 ± 5 nm light emitting diode (LED) on Escherichia coli O157:H7, Salmonella Typhimurium and Shigella sonnei . Its antibacterial mechanism was also investigated by determining the permeability of...
Fri, 07/03/2015 - 01:23
Publication date: Available online 2 July 2015 Author(s): Renhui Huang , Xueying Tao , Cuixiang Wan , Shengjie Li , Hengyi Xu , Feng Xu , Nagendra P. Shah , Hua Wei Lactobacillus plantarum ZDY 2013, a novel strain isolated from Chinese traditional fermented acid beans, was systematically evaluated for its survival capacity under stress conditions (pH, bile salt, simulated...
Tue, 06/30/2015 - 01:19
Street vended foods are the vehicle of pathogens transmission resulting in foodborne diseases. The purpose of this paper is to propose a conceptual public health risks framework of consuming road vended foods based on detailed review of existing literature in developing countries. Although street food is enjoying a wide range of acceptability, microorganisms from the genus Campylobacter,...
Mon, 06/29/2015 - 01:25
Aims The presence of viable but nonculturable (VBNC) bacterial pathogens which often fail to be detected by cultivation and can regain the cultivability if the living conditions improve were reported. The objective of this study is to determine the occurrence of VBNC Salmonella spp. and Shigella spp. in the biosolids during anaerobic digestion and its reactivation during the cake storage....
Wed, 06/24/2015 - 06:20
Few studies within the pathogenic field have used advanced imaging and analytical tools to quantitatively measure pathogenicity in vivo. In this work, we present a novel approach for the investigation of host–pathogen processes based on medium-throughput 3D fluorescence imaging. The guinea pig model for Shigella flexneri invasion of the colonic...
Fri, 06/19/2015 - 01:27
Publication date: July 2015 , Volume 98, Issue 7 Author(s): Xin Wang , Qinglong Wu , Kan Deng , Qiang Wei , Ping Hu , Jingjing He , Huan Liu , Yong Zheng , Hua Wei , Nagendra P. Shah , Tingtao Chen To screen for potential probiotics with high adhesion capability, a chemostat model-based cultured human feces and denaturing gradient gel electrophoresis methods were applied, and the...
Fri, 06/12/2015 - 03:20
Enteroaggregative and uropathogenic Escherichia coli, Shigella flexneri 2a, and the hybrid enteroaggregative/Shiga toxin–producing E. coli strain (O104:H4) are important pathogens responsible for intestinal and urinary tract infections, as well as sepsis and hemolytic uremic syndrome. They have in common the production of a serine protease called Pic. Several biological roles for Pic have...
Fri, 05/22/2015 - 01:19
Publication date: Available online 21 May 2015 Author(s): G.N. Na , S.A. Kim , O.C. Kwon , M.S. Rhee The aim of this study was to develop a new selective and differential medium for isolating Shigella sonnei (designated 3SD medium). The new medium was based on three carbohydrates (lactose, sorbitol, and xylose) and a chromogenic substrate (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside...
Fri, 04/17/2015 - 03:20
Diarrhea due to enteric bacterial pathogens causes significant morbidity and mortality in the United States and worldwide. However, bacterial pathogens may be infrequently identified. Currently, culture and enzyme immunoassays (EIAs) are the primary methods used by clinical laboratories to detect enteric bacterial pathogens. We conducted a multicenter evaluation of the BD Max enteric bacterial...
Tue, 04/14/2015 - 01:18
Autotransporters are a superfamily of virulence factors secreted by Gram negative bacteria. They are comprised of an N-terminal passenger domain that is translocated across the outer membrane, and a C-terminal domain that inserts into the outer membrane forming a β-barrel anchor. It is still poorly understood how the passenger is efficiently translocated in the absence of external energy inputs....
Fri, 01/30/2015 - 01:18
Sergio Sánchez, María Teresa Llorente, María Aurora Echeita, Silvia Herrera-León Escherichia coli serogroups O5, O15, O26, O45, O55, O76, O91, O103, O104, O111, O113, O118, O121, O123, O128, O145, O146, O157, O165, O172, and O177 are the O-antigen forms of the most clinically relevant Shiga toxin-producing E. coli (STEC) serotypes. In this study, three multiplex PCR assays able to specifically...
Wed, 01/21/2015 - 01:16
Publication date: 2 February 2015 , Volume 194 Author(s): Mohamed K. Omer , Sigrun J. Hauge , Øyvin Østensvik , Birgitte Moen , Ole Alvseike , Ole-Johan Røtterud , Miguel Prieto , Sissel Dommersnes , Ole H. Nesteng , Truls Nesbakken The aims of this study were to investigate bacterial dynamics in the sheep meat chain, from fleece to meat trimmings, using both quantitative and...
Wed, 01/21/2015 - 01:16
Publication date: January 2015 , Volume 305, Issue 1 Author(s): Cecilia Ambrosi , Monica Pompili , Daniela Scribano , Dolores Limongi , Andrea Petrucca , Sonia Cannavacciuolo , Serena Schippa , Carlo Zagaglia , Milena Grossi , Mauro Nicoletti Through the action of the type three secretion system (T3SS) Shigella flexneri delivers several effectors into host cells to promote cellular...
Tue, 01/20/2015 - 14:53
Publication date: February 2015 , Volume 109 Author(s): Noriko Goji , Amit Mathews , George Huszczynski , Chad R. Laing , Victor P.J. Gannon , Morag R. Graham , Kingsley K. Amoako Shiga toxin (stx)-producing Escherichia coli (STEC) contamination in food and water is one of the most recognized concerns and a major financial burden in human hygiene control worldwide. Rapid and highly...
Tue, 01/20/2015 - 14:53
Paediatric acute gastroenteritis is a global public health problem.Comprehensive laboratory investigation for viral, bacterial and parasitic agents is helpful for improving management of acute gastroenteritis in health care settings and for monitoring and controlling the spread of these infections.Our study aimed to investigate the role of various pathogens in infantile diarrhoea in Bulgaria...

Pages