Staphylococcus aureus

Thu, 01/21/2021 - 02:02
Abstract Fresh-cut fruits are susceptible for microbial contamination during handling and storage. Hence, there is a need for minimal processing of such foods using non-thermal technology that can inactivate both bacterial pathogens and undesirable enzymes while retaining the quality. In this regard, synergistic effect of light emitting diode (LED) based blue light (BL) and natural exogenous...
Thu, 01/21/2021 - 00:00
Methicillin-resistant Staphylococcus aureus (MRSA) strains have veterinary and public health importance as they are responsible for a wide range of difficult to treat infections and food poisoning. Two hundred samples (50 samples each of minced meat, beef luncheon, Karish cheese, and human samples (pus swab from open wounds)) were cultured, and MRSA strains were identified using disk diffusion...
Thu, 01/21/2021 - 00:00
Daptomycin is an important antibiotic for the treatment of infections caused by Staphylococcus aureus. The emergence of daptomycin resistance in S. aureus is associated with treatment failure and persistent infections with poor clinical outcomes. Here, we investigated host innate immune responses against clinically derived, daptomycin-resistant (DAP-R) and -susceptible S. aureus paired isolates...
Wed, 01/20/2021 - 22:13
The medicinal plants, like Alhagi maurorum, has received great attentions, especially by food scientists and manufacturers as natural food preservatives due to their antimicrobial and antioxidant activities. In this study, the effects of extracting solvents, namely, hexane, water and acetone, individually or in combination to each other according to especial cubic mixture design, as extracting...
Wed, 01/20/2021 - 16:00
Staphylococcus aureus sequence type 72 (ST72) is a major community-associated (CA) methicillin-resistant Staphylococcus aureus (MRSA) that has rapidly entered the hospital setting in Korea, causing mild superficial skin wounds to severe bloodstream infections. In this study, we sequenced and analyzed the genomes of one methicillin-resistant human isolate and one methicillin-sensitive human...
Wed, 01/20/2021 - 00:00
The article describes activities of an antibiotic center at a university hospital in the Czech Republic and presents the results of antibiotic stewardship program implementation over a period of 10 years. It provides data on the development of resistance of Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus to selected antibiotic agents as well as...
Tue, 01/19/2021 - 04:02
Nowadays, films with antibacterial activity and applied for freshness monitoring by colorimetric response have been drawing growing attention in food packaging. However, the development of versatile antibacterial and colorimetric agents is still highly desirable. Herein, WO3 nanorods are incorporated in a polyvinyl alcohol (PVA) matrix to develop a novel composite film with photothermal...
Tue, 01/19/2021 - 00:00
Multidrug-resistant (MDR) bacteria are increasing due to the abuse and misuse of antibiotics, and nosocomial infections by MDR bacteria are also increasing. The aim of this study was to identify new substances that can target MDR bacteria among 12 plant extracts that are known to have antibacterial effects. The experiments were performed by the disk diffusion test and microdilution minimum...
Thu, 01/14/2021 - 16:00
BackgroundStaphylococcus aureus is a leading cause of bacterial bloodstream infections. The heterogeneity in patient outcomes in S. aureus bacteremia (SAB) can be attributed in part to strain characteristics, which may influence host response to infection. We specifically examined the relationship between lipoteichoic acid (LTA) release from S. aureus and disease phenotype, strain background, and...
Wed, 01/13/2021 - 06:09
Author(s): Wen-Li Seow, Nor Ainy Mahyudin, Syafinaz Amin-Nordin, Son Radu, Noor Azira Abdul-Mutalib The purpose of the study was to identify the prevalence of Staphylococcus aureus and its antimicrobial resistance profile among food handlers and in cooked food in Klang Valley, Malaysia. The correlation between the prevalence of S. aureus and the occupational information of the food handlers was...
Wed, 01/13/2021 - 02:02
Deoxynivalenol (DON) is a widely distributed mycotoxin that frequently occurs in various foodstuffs, and poses a health risk to human and animals. Biodegradation of DON to less- or non-toxic substances using naturally existing microorganisms is considered the best approach for DON detoxification. Although various microorganisms capable of detoxifying DON have been reported; however, such studies...
Wed, 01/13/2021 - 02:02
Salted duck eggs as a convenient food are very popular in China and Southeast Asia. Generally, they are produced by traditional curing methods. Here we used traditional methods to profile the bacterial community of salted duck eggs purchased from markets to systematically investigate their microbiological safety. 77 bacteria belonging to 14 genera were isolated. Bacillus related to flavor...
Tue, 01/12/2021 - 22:13
The antibacterial effect of volatile compounds in millets and cereal by‐products was investigated. Hexane extracts of rice bran (RB), corn cob (CC), green (GM), and yellow millets (YM) showed antibacterial activities against Escherichia coli ATCC 35150, Bacillus cereus ATCC 14579, Helicobacter pylori MH179991, and Staphylococcus aureus ATCC 13150 with minimum inhibitory concentration (MIC)...
Mon, 01/11/2021 - 22:13
Disk diffusion method to determine antimicrobial activity of plant essential oils was used. While the essential oil extracted from oregano has a high effect on 9 of our test microorganisms (75%), one of them had a medium effect and two of them had a weak effect in the test results. While the essential oil obtained from thyme was effective on VRE, MSSA, Escherichia feacalis and Staphylococcus...
Mon, 01/11/2021 - 16:00
The lesser-known traditionally processed meat products such as beef kargyong, pork kargyong, satchu, and khyopeh are popular food items in the Himalayan state of Sikkim in India. The present study aimed to assess the microbiological safety of traditional meat products by identifying the potential spoilage or pathogenic bacteria, detecting the enterotoxins, and screening the antibiotic...
Sun, 01/10/2021 - 22:12
Exudative epidermatitis (EE) or greasy pig disease (GPD) is a contagious disease of pig and endemic worldwide caused by toxigenic strains under genus Staphylococcus. The present study reported an outbreak of GPD in Champhai district of Mizoram adjoining to the southern border of Myanmar. A total of 60 samples were collected from 22 clinically affected animals and processed for isolation and...
Sun, 01/10/2021 - 22:09
BACKGROUND Fermentation, a traditional food preserving technique and an effective process, widely used to enrich the nutrients diversity and bioactivity of the fermented foods since ancient times. This study aimed at investigating the effects of various fermentation starters on the physicochemical, antioxidant, antimicrobial, and antidiabetic properties of the blueberry juices. The blueberry...
Fri, 01/08/2021 - 16:00
Methicillin-resistant Staphylococcus aureus (MRSA) have been found on German dairy farms and may be the cause of difficult-to-treat bovine mastitis. Considering the one health approach, MRSA might be transmitted from animals to humans raising the risk for severe infections. On 17 German dairy farms with a history of MRSA detection, MRSA strains were isolated from quarter milk, bulk tank milk, and...
Thu, 01/07/2021 - 22:14
Animal petting zoos and farm fairs provide the opportunity for children and adults to interact with animals, but contact with animals carries a risk of exposure to zoonotic pathogens and antimicrobial‐resistant bacteria. The aim of this study was to assess the occurrence of Shiga toxin‐producing Escherichia coli (STEC), Salmonella, extended‐spectrum β‐lactamase (ESBL)‐producing Enterobacteriaceae...
Thu, 01/07/2021 - 16:00
Formation of viable but non-culturable (VBNC) status in methicillin-resistant Staphylococcus aureus (MRSA) has never been reported, and it poses a significant concern for food safety. Thus, this study aimed to firstly develop a rapid, cost-effective, and efficient testing method to detect and differentiate MRSA strains in the VBNC state and further apply this in real food samples. Two targets...
Thu, 01/07/2021 - 06:15
Author(s): Yu-gang Shi, Run-run Zhang, Chen-min Zhu, Ming-feng Xu, Qing Gu, Rammile Ettelaie, Shan Lin, Yi-fan Wang, Xin-yi Leng The objective of this study was to investigate the antibacterial activity and potential mechanism of alkyl gallates against Escherichia coli and Staphylococcus aureus. Results show that the length of the alkyl chain plays a pivotal role in eliciting the activity and...
Thu, 01/07/2021 - 02:02
Active films have gained increasing interest because of their potential to reduce the amount of additives into foods. The aim of this work was to produce and characterize bi-layer films based on gelatin in both layers, in which the nisin and/or “pitanga” leaf hydroethanolic extract was loaded in the second layer. The bi-layer gelatin-based films were characterized regarding humidity and...
Wed, 01/06/2021 - 02:03
The study evaluates the effect of Artemisia dracunculus essential oil (EO) on two pathogenic bacteria Salmonella enterica serovar Typhimurium and Staphylococcus aureus and Vero cell line. To evaluating the anti-biofilm potential of the EO, a microtiter-plate test (MtP) and scanning electron microscopy (SEM) were performed. The quorum-sensing inhibitory properties were examined by QS-related gene...
Wed, 01/06/2021 - 02:02
In recent years, there has been a serious need to develop and test new biodegradable packaging. Therefore, furcellaran (FUR) and whey protein isolate (WPI) films containing pu-erh (PE) or green tea (GT) extracts were prepared in this study. The effect of the extracts on the structure, rheological and mechanical properties, and antioxidant and antimicrobial activity of FUR/WPI films was tested....
Tue, 01/05/2021 - 22:10
(‐)‐Epigallocatechin gallate (EGCG) exhibits multiple biological activities, but unfortunately with poor stability. Poly(butyl‐2‐cyanoacrylate) (PBCA) nanoparticles have been widely elaborated for nearly 50 years. However, PBCA‐based nanowires (PNWs) were reported scarcely. Here, EGCG‐encapsulated PNWs (EGCG@PNWs) were successfully constructed. Employing PNWs as a delivery material, 2.0 mg EGCG...
Tue, 01/05/2021 - 10:02
The occurrence of viable-but-nonculturable (VBNC) bacteria poses a potential risk to food safety due to failure in conventional colony detection. In this study, induction of VBNC Staphylococcus aureus was conducted by exposure to an atmospheric-pressure air dielectric barrier discharge-nonthermal-plasma (DBD-NTP) treatment with an applied energy of 8.1 kJ. The stress resistance profiles and...
Mon, 01/04/2021 - 22:13
This present work shows the potential of dimethyl dicarbonate (DMDC) as a promising approach to ensure quality and safety of coconut water in term of microbial inactivation and minimal changes in quality parameters during cold storage (4 °C). Pathogenic strains including Escherichia coli, Staphylococcus aureus, Salmonella Enteritidis and Listeria monocytogenes were selected for evaluation of...
Mon, 01/04/2021 - 02:02
Gluten-free biscuits were developed with the addition of chia seeds (Salvia hispanica L.) and turmeric powder. An experimental design 22 were employed in the formulation optimization that promotes better sensory acceptance through acceptance test with a hedonic scale of 9 points. For characterization purposes, the centesimal composition from chia seeds and the turmeric powder were determined. The...
Sat, 01/02/2021 - 22:10
Aims To perform the molecular characterization of 23 Staphylococcus aureus isolates from pigs with signs of infections recovered in Spanish farms during 2018‐2019. Methods and Results The antimicrobial resistance pattern and virulence profile were determined. The molecular typing was performed by different molecular techniques. The transferability of the cfr gene was assessed by conjugation and...
Fri, 01/01/2021 - 22:10
Staphylococcus aureus, an opportunistic pathogen, causes diverse community and nosocomial acquired human infections, including folliculitis, impetigo, sepsis, septic arthritis, endocarditis, osteomyelitis, implant associated biofilm infections and contagious mastitis in cattle. In recent days both methicillin‐sensitive and methicillin resistant S. aureus infections have increased. Highly...
Wed, 12/30/2020 - 00:00
Avocado Hass (Persea americana Mill) peel extract (APE) has the potential as a natural ingredient to substitute for chemical preservatives. The objectives of this study were to assess the phytochemical composition by high-performance liquid chromatography–quadrupole time-of-flight mass/mass spectrometry (HPLC-qTOF-MS/MS), total phenolic content (TPC), proanthocyanidin (PAC) content, and...
Sun, 12/27/2020 - 04:02
Rhamnolipid is the main group of biosurfactants predominantly produced by Pseudomonas aeruginosa, a ubiquitous and opportunistic pathogen, which limits its large-scale exploitation. Thus, cost-effective rhamnolipid production from a newly isolated nonpathogenic Enterobacter sp. UJS-RC was investigated. The highest rhamnolipid production (4.4 ± 0.2 g/L) was achieved in a medium constituting...
Fri, 12/25/2020 - 06:10
Author(s): Xiaonan Zhao, Xiaomeng Yuan, Ming Hu, Yin Zhang, Lulu Li, Qing Zhang, Xuexia Yuan, Wenbo Wang, Yuqing Liu Staphylococcus aureus (S. aureus) is one of the most common pathogens that cause mastitis in dairy cows. The emergence of methicillin-resistant S. aureus (MRSA) constitutes a serious public health concern due to its ability to colonize and infect humans and animals. In this study,...
Thu, 12/24/2020 - 12:00
More than 3 decades have passed since infection control was implemented nationwide in China in 1986. A comprehensive set of regulations and guidelines has been developed, and almost all hospitals have established infection control teams. However, compliance is variable and is usually suboptimal. The incidence of certain multidrug-resistant organisms (MDROs), including carbapenem-resistant...
Thu, 12/24/2020 - 08:04
Staphylococcus aureus is one of the most common mastitis-causing bacteria in dairy cows. It is associated with reduced production performance in animals and with huge financial losses for the dairy industry worldwide. An accurate and sensitive method for the early diagnosis and identification of Staph. aureus in milk samples is essential. The present study aimed to establish a closed-tube...
Thu, 12/24/2020 - 08:04
Staphylococcus aureus is one of the most frequent pathogens causing intramammary infections in dairy herds. Consequently, virulence factors, pathobiology, and epidemiology of Staphylococcus aureus strains have been widely assessed through the years. Nevertheless, not much has been described about the epidemiology of Staph. aureus strains from bulk tank milk (BTM) and adherences on milking...
Thu, 12/24/2020 - 08:04
Staphylococcus aureus can elicit mild to more severe degrees of mastitis in cattle, depending on the response of the host's immune system and the virulence factors of the specific isolate. Several virulence factors are controlled by a global regulatory system, designated accessory gene regulator (agr). Thus, the objective was to examine associations between different capsular and agr types and...
Thu, 12/24/2020 - 08:04
Staphylococcus aureus is a widespread foodborne pathogen that threatens human health. In particular, multidrug-resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA) are emerging problems in modern health care, food safety, and animal health, which require the development of new antimicrobials to replace overused conventional antibiotics. Dairy products can potentially act...
Wed, 12/23/2020 - 12:00
ABSTRACT The aim of this study was to illustrate the relative pervasiveness of Borderline Oxacillin Resistant Staphylococcus aureus (BORSA) and Methicillin-Resistant Staphylococcus aureus (MRSA) in community and food of animal origin and their relationship with other genetic determinants. Staphylococcus aureus isolates were subjected to E-test using the antibiotics: oxacillin, ceftriaxone,...
Sun, 12/20/2020 - 22:10
Aims To evaluate the applicability of the Mimosa tenuiflora and Eucalyptus urograndis pyroligneous acids (PAs) as alternative antiseptics in dairy goats. Methods and Results Cytotoxicity was evaluated in vitro using bacteria, as well as in vivo using goats, and the influence of PAs on the physicochemical parameters of fresh milk were examined. The cytotoxicity of PAs was evaluated in terms of...
Sat, 12/19/2020 - 00:00
The present research aimed to evaluate the antibacterial activity of volatile organic compounds (VOCs) produced by octocoral-associated bacteria Bacillus sp. BO53 and Pseudoalteromonas sp. GA327. The volatilome bioactivity of both bacteria species was evaluated against human pathogenic antibiotic-resistant bacteria, methicillin-resistant Staphylococcus aureus, Acinetobacter baumanni, and...
Fri, 12/18/2020 - 04:03
This study presents a method for modifying pectin with phenolic acids catalyzed by lipase in a two-phase system of water/tetrahydrofuran. Salicylic acid (SA) and its isomers, including m-hydroxybenzoic acid (MHBA) and p-hydroxybenzoic acid (PHBA), were grafted onto pectin, and the products were characterized via UV–vis, Fourier transform infrared spectroscopy (FTIR), and 1H NMR analyses to...
Thu, 12/17/2020 - 10:02
Bacterial pathogens are rapidly evolving resistance to all clinically available antibiotics. One part of the solution to this complex issue is to better understand the resistance mechanisms to new and existing antibiotics. Here, we focus on two antibiotics. Teixobactin is a recently discovered promising antibiotic that is claimed to "kill pathogens without detectable resistance" (L. L. Ling, T....
Thu, 12/17/2020 - 10:02
Current guidelines recommend vancomycin and linezolid as first-line agents against methicillin-resistant Staphylococcus aureus (MRSA) nosocomial pneumonia. Telavancin is a potential new therapeutic alternative, specifically in monomicrobial MRSA pneumonia. This study compared the efficacies of telavancin versus linezolid in a porcine model of severe MRSA pneumonia. In 18 mechanically ventilated...
Tue, 12/15/2020 - 00:00
Bacteriophage endolysins have attracted attention as promising alternatives to antibiotics, and their modular structure facilitates endolysin engineering to develop novel endolysins with enhanced versatility. Here, we constructed hybrid proteins consisting of two different endolysins for simultaneous control of two critical foodborne pathogens, Staphylococcus aureus and Bacillus cereus. The full-...
Mon, 12/14/2020 - 00:00
Native egg albumin (NEA) was isolated from hen eggs and hydrolyzed by pepsin to produce hydrolyzed egg albumin (HEA). HEA was chemically characterized and screened for its antibacterial activity against 10 pathogenic bacteria (6 Gram (+) and 4 Gram (−)). The SDS-PAGE pattern of NEA showed molecular weights of hen egg albumin subunits ranging from 30 to 180 kDa. The highest intensive...
Sat, 12/12/2020 - 00:00
The emergence of drug-resistant pathogens leads to a gradual decline in the efficacy of many antibacterial agents, which poses a serious problem for proper therapy. Multidrug resistance (MDR) mechanisms allow resistant bacteria to have limited uptake of drugs, modification of their target molecules, drug inactivation, or release of the drug into the extracellular space by efflux pumps (EPs). In...
Fri, 12/11/2020 - 22:09
The development of natural antimicrobial agents has attracted long‐term attention due to the increasing demand for food preservation. Papaya, a widely cultivated nutritious tropical fruit, has benzyl isothiocyanate (BITC) as one of the most important secondary metabolites in its seeds. And the antibacterial activity of BITC toward different strains and the main antibacterial pathway remain...
Fri, 12/11/2020 - 22:08
This study aimed to characterize yerba‐maté kombucha by showing its oxidative stress inhibitory property with Saccharomyces cerevisiae yeast as the model and to determine its antibacterial activity against Staphylococcus aureus and Escherichia coli. Different yerba‐maté concentrations and fermentation temperatures were analyzed by an experimental design. Sucrose was used as the carbon source and...
Wed, 12/09/2020 - 10:01
ABSTRACT Genotyping of 2,882 Pseudomonas aeruginosa isolates that had been collected during the last 40 years identified the ExoU-positive lineages PA14 (ST253) and ST235 as the second and third most frequent clones in the P. aeruginosa population. Both clones were approximately 2-fold more frequently detected in animate habitats than in soil or aquatic habitats. While ST253 clone isolates were...

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