Staphylococcus aureus

Fri, 07/03/2020 - 16:00
Bacterial microorganisms which are latent in in vitro cultures can limit the efficiency of in vitro methods for the conservation of genetic resources. In this study we screened 2,373 accessions from the in vitro sweetpotato germplasm collection of the International Potato Center in Lima, Peru for bacteria associated with plantlets in tissue culture through a combination of morphological methods...
Thu, 07/02/2020 - 10:03
ABSTRACT Since the late 1990s, changes in the epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) were recognized with the emergence of community-associated MRSA (CA-MRSA). CA-MRSA belonging to clonal complex 152 (CC152), carrying the small staphylococcal cassette chromosome mec (SCCmec) type V and encoding the Panton-Valentine leukocidin (PVL), has been observed in Europe. The aim...
Thu, 07/02/2020 - 08:02
Staphylococcus aureus is an important cause of bovine mastitis, and intramammary infections caused by this pathogen are often characterized as mild, chronic, or persistent. The strains of Staph. aureus associated with mastitis belong to several distinct bovine-adapted bacterial lineages. Studies of host-pathogen interactions have demonstrated that significant differences exist between Staph....
Wed, 07/01/2020 - 22:16
Eudragit RS100 nanoparticles containing Baccharis dracunculifolia DC essential oil (N‐EO) was developed by the nanoprecipitation method. N‐EO presented an average diameter of 151.6 nm, zeta potential (ζ) of +51.7 mV, and encapsulation efficiency of 99.4%. The antibacterial activity of N‐EO and free essential oil (EO) was evaluated in BHI broth against Staphylococcus aureus, Bacillus cereus,...
Wed, 07/01/2020 - 08:02
by Yemane Weldu, Mulugeta Naizgi, Amanuel Hadgu, Abraham Aregay Desta, Amlsha Kahsay, Letemichael Negash, Genet Gebrehiwet Hailu, Araya Gebreyesus Wasihun Background Neonatal septicemia is a life threatening medical emergency that requires timely detection of pathogens with urgent rational antibiotics therapy. Methods A cross-sectional study was conducted between March 2017 to September 2018...
Tue, 06/30/2020 - 16:00
Recent in vitro studies reported the exceptional ability of some bacterial species to form biofilm-like aggregates in human and animal synovial fluids (SF), but evidences from infected clinical samples are still lacking. In this study, we investigated whether this bacterial phenotype was present in infected SFs collected from joint infections and if it was maintained in in vitro settings. SFs...
Tue, 06/30/2020 - 10:03
ABSTRACT Efflux is an important mechanism in Gram-negative bacteria conferring multidrug resistance. Inhibition of efflux is an encouraging strategy to restore the antibacterial activity of antibiotics. Chlorpromazine and amitriptyline have been shown to behave as efflux inhibitors. However, their mode of action is poorly understood. Exposure of Salmonella enterica serovar Typhimurium and...
Tue, 06/30/2020 - 10:03
ABSTRACT Staphylococcus aureus infection is a major public health threat in part due to the spread of antibiotic resistance and repeated failures to develop a protective vaccine. Infection is associated with production of virulence factors that include exotoxins that attack host barriers and cellular defenses, such as the leukocidin (Luk) family of bicomponent pore-forming toxins. To investigate...
Tue, 06/30/2020 - 10:03
ABSTRACT Staphylococcus aureus has recently overtaken Pseudomonas aeruginosa as the most commonly recognized bacterial pathogen that infects the respiratory tracts of individuals with the genetic disease cystic fibrosis (CF) in the United States. Most studies of S. aureus in CF patient lung infections have focused on a few isolates, often exclusively laboratory-adapted strains, and how they are...
Tue, 06/30/2020 - 10:03
ABSTRACT Resistance or tolerance to traditional antibiotics is a challenging issue in antimicrobial chemotherapy. Moreover, traditional bactericidal antibiotics kill only actively growing bacterial cells, whereas nongrowing metabolically inactive cells are tolerant to and therefore "persist" in the presence of legacy antibiotics. Here, we report that the diarylurea derivative PQ401, previously...
Sat, 06/27/2020 - 22:15
The antimicrobial calcium‐alginate‐based films containing the self‐microemulsifying (SME) thyme essential oil (TEO) formulations were fabricated successfully. A Ca‐alginate film containing nano‐emulsified TEO as well as a neat Ca‐alginate film were considered as the controls. The SME films released the TEO completely within 155 min and inhibited the growth of S. aureus and E. coli in in vitro...
Fri, 06/26/2020 - 22:12
Aims Biological techniques can manage plant diseases safely and in environmentally friendly ways, but their efficacy needs improvement. It is of the utmost importance to search for powerful microbes for the effective control of plant diseases. Methods and Results Unheated self‐digestive solutions (SDS) that were heated at 100°C for 30 minutes (H‐SDS) or stored for 12 months at room temperature (S...
Fri, 06/26/2020 - 16:00
Staphylococcus aureus is a major cause of hospital- and community-acquired infections placing a significant burden on the healthcare system. With the widespread of multidrug-resistant bacteria and the lack of effective antibacterial drugs, fosfomycin has gradually attracted attention as an “old drug.” Thus, investigating the resistance mechanisms and epidemiology of fosfomycin-resistant S. aureus...
Wed, 06/24/2020 - 16:00
BackgroundThe prevalence of colonization with multidrug-resistant organisms (MDROs) among healthy adults in the community is largely unknown. This study investigated the colonization rate of multidrug-resistant Enterobacteriaceae, methicillin-resistant Staphylococcus aureus (MRSA), and vancomycin-resistant enterococci (VRE) in the community in Taiwan, and compared the gut microbiota between MDRO...
Wed, 06/24/2020 - 16:00
Staphylococcus aureus is a leading cause for clinical infections and food intoxications, causing over 100,000 yearly cases of bacteremia in the United States and 434 food-borne outbreaks in the European Union. Approximately 30% of the population permanently carry S. aureus asymptomatically in their nasal cavity. The risk of infection and transmission to food items or the environment is higher in...
Wed, 06/24/2020 - 10:02
Methicillin-resistant Staphylococcus aureus (MRSA) has grown to become a major burden on health care systems. The cumulation of limited therapeutic options and worsened patient outcomes with persistent MRSA bacteremia has driven research in optimizing its initial management. The guidelines published by the Infectious Diseases Society of America currently recommend combination therapy for...
Wed, 06/24/2020 - 10:02
Two nonamidated host defense peptides named Pin2[G] and FA1 were evaluated against three types of pathogenic bacteria: two (Staphylococcus aureus UPD13 and Pseudomonas aeruginosa UPD3) isolated from diabetic foot ulcer patients, and another (Salmonella enterica serovar Typhimurium [ATCC 14028]) from a commercial collection. In vitro experiments showed that the antimicrobial performance of the...
Wed, 06/24/2020 - 06:11
Author(s): Jin Hwa Park, Mi Seon Kang, Kyung Min Park, Hee Young Lee, Gyeong Sik Ok, Min Seon Koo, Seok In Hong, Hyun Jung Kim The present study aimed to develop and validate a predictive model of Salmonella spp. and Staphylococcus aureus growth in fresh egg under isothermal and non-isothermal (fluctuating temperature) conditions and also estimate infection probability based on Monte Carlo...
Tue, 06/23/2020 - 22:14
The objective of this study was to evaluate the possible synergistic interactions on antibacterial and antioxidant efficacy of two plant essential oils (EOs) Eucalyptus caesia Benth and Dracocephalum multicaule Montbr & Auch in vitro and in pear slices. Antibacterial combination effect was evaluated against seven important pathogenic bacteria (Bacillus cereus, Listeria monocytogenes,...
Tue, 06/23/2020 - 08:02
by Catherine E. Foster, Melissa Kok, Anthony R. Flores, Charles G. Minard, Ruth A. Luna, Linda B. Lamberth, Sheldon L. Kaplan, Kristina G. Hulten Background Microbial surface component recognizing adhesive matrix molecules (MSCRAMMs) facilitate Staphylococcus aureus adherence to host tissue. We hypothesized that S. aureus isolates from implant-associated infections (IAIs) would differ in MSCRAMM...
Mon, 06/22/2020 - 06:10
Author(s): Apostolos S. Angelidis, Dimitrios Komodromos, Rebecca Giannakou, Georgios Arsenos, Athanasios I. Gelasakis, Maria Kyritsi, George Filioussis, Christos Hadjichristodoulou, Pavlina Torounidou, Anna Papa, Daniel Sergelidis The presence of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in raw milk is a challenge for veterinarians and public health professionals. In this...
Fri, 06/19/2020 - 08:02
Farmers prefer fast, sensitive, and on-site tests for treatment decisions on mastitis. Due to the time to results of the currently available diagnostic tools, these are rarely used for that purpose. Genotypic tests that do not require a growth step may be suitable for on-site testing, for example loop-mediated isothermal amplification (LAMP), which has been described as a sensitive test that can...
Thu, 06/18/2020 - 22:14
The aims of the present study were to examine the occurrence of Staphylococcus spp. in the tonsils of slaughtered pigs in a regional slaughterhouse in Greece, the antibiotic resistance of the Staphylococcus spp. isolates, and the enteroxigenicity of the S. aureus isolates. Staphylococcus spp. were isolated in 70 (48.61%) out of the total 144 tonsil samples. The predominant species was S. aureus...
Thu, 06/18/2020 - 10:02
Livestock-associated methicillin-resistant Staphylococcus aureus sequence type (ST) 398 (LA-MRSA ST398) is a genetic lineage for which pigs are regarded as the main reservoir. An increasing prevalence of LA-MRSA ST398 has been reported in areas with high livestock density throughout Europe. In this study, we investigated the drivers contributing to the introduction and spread of LA-MRSA ST398...
Thu, 06/18/2020 - 02:02
Sapota (Manilkara Zapota) belongs to the Sapotaceae family and is one of the sweetest, delicious, and popular tropical fruits with high nutritional benefits. Due to its high water content (78%), it is easily damaged, which in turn reduces its shelf life. Also, significant post-harvest losses occur due to insufficient storage, poor handling, and transportation conditions, which also increase the...
Tue, 06/16/2020 - 00:00
Thioridazine hydrochloride (HCl) has been suggested as a promising antimicrobial helper compound for the treatment of infections with antimicrobial-resistant bacteria. Unfortunately, the therapeutic concentration of thioridazine HCl is generally higher than what can be tolerated clinically, in part due to its toxic side effects on the central nervous system. Therefore, we aimed to synthesize a...
Fri, 06/12/2020 - 22:15
Bacterial toxins are food safety hazards causing about 10% of all reported foodborne outbreaks in Europe. Pertinent to Gram‐positive pathogens, the most relevant toxins are emetic toxin and diarrheal enterotoxins of Bacillus cereus, neurotoxins of Clostridium botulinum, enterotoxin of Clostridium perfringens, and a family of enterotoxins produced by Staphylococcus aureus and some other...
Wed, 06/10/2020 - 08:02
Diabetic foot infection is among the most common complications of diabetes mellitus which significantly causes hospitalization and is the most prevalent etiology of nontraumatic amputation worldwide. The current study aimed at assessing the frequency and antimicrobial susceptibility patterns of diabetic foot infection of patients from the Bandar Abbas area, in the south of Iran. In this study, a...
Tue, 06/09/2020 - 22:17
Chitosan (CHI) and whey protein are usually used to prepare edible films for food preservation. However, the composite film composed of the two components does not yield satisfactory properties for chestnut preservation. In this study, nano‐cellulose and cinnamaldehyde (CMA) were added to CHI and whey protein, creating a new composite film with strong water retention, bacteriostatic, and...
Tue, 06/09/2020 - 06:13
Author(s): Ligia E. Prezzi, Sarah H.I. Lee, Valéria M.R. Nunes, Carlos H. Corassin, Tatiana C. Pimentel, Ramon S. Rocha, Gustavo L.P. Ramos, Jonas T. Guimarães, Celso F. Balthazar, Maria Carmela K.H. Duarte, Mônica Q. Freitas, Erick A. Esmerino, Marcia C. Silva, Adriano G. Cruz, Carlos A.F. Oliveira This study aimed to evaluate the effect of Lactobacillus rhamnosus GG on growth of Staphylococcus...
Mon, 06/08/2020 - 04:03
Viridicatumtoxins are a rare class of tetracycline-like antibiotics that strongly inhibit drug-resistant Gram-positive bacteria. Although reported to exhibit in vitro inhibition activity to undecaprenyl pyrophosphate synthase (UPPS), an essential enzyme in bacterial cell wall synthesis, the biological targets and mechanism of action of viridicatumtoxins, especially the drug–target interactions,...
Sun, 06/07/2020 - 04:03
Staphylococcus aureus is notoriously known for its rapid development of resistance to conventional antibiotics. S. aureus can alter its membrane composition to reduce the killing effect of antibiotics and antimicrobial peptides (AMPs). To obtain a more complete picture, this study identified the resistance genes of S. aureus in response to human cathelicidin LL-37 peptides by screening the...
Sat, 06/06/2020 - 08:02
by Awwad Radwan, Gamal M. Mahrous Bacterial histidine kinases (HKs) are considered attractive drug targets because of their ability to govern adaptive responses coupled with their ubiquity. There are several classes of HK inhibitors; however, they suffer from drug resistance, poor bioavailability, and a lack of selectivity. The 3D structure of Staphylococcus aureus HK was not isolated in high-...
Sat, 06/06/2020 - 04:03
One of the most effective methods for increasing the antimicrobial activity of a substance is to combine it with one or more other antimicrobial agents. The aim of the present study was to evaluate the antimicrobial effect of buforin I and nisin alone and investigate the synergistic action of these compounds against the most important food spoilage microorganisms in clouding B. subtilis, S....
Sat, 06/06/2020 - 04:03
High moisture slurries used in production of table spreads may permit growth of  Staphylococcus aureus and  Bacillus cereus  and subsequent production of heat stable enterotoxin. Compliance with the Food Safety Modernization Act (FSMA), specifically 21 CFR Part 117, Subpart B and Section 117.8 (c)(2) and (c)(3) requires a hazard analysis to determine if preventive controls are needed. In this...
Sat, 06/06/2020 - 04:03
To reduce the number of potential S. aureus contaminated cheese reaching consumers, European legislation stipulates that all cheese must be tested for coagulase-positive staphylococci (CPS) at the point in production when numbers are expected to be highest. If CPS counts exceed 105 CFU/mL, enterotoxin tests must be conducted. In the case the enterotoxin test shows positive results the cheese must...
Thu, 06/04/2020 - 03:38
Staphylococcus aureus is a major foodborne pathogen that causes severe disease in humans. It is commonly found in milk and dairy products, particularly in fresh brined cheese. Our aim was to investigate the behavior of Staph. aureus and enterotoxin production during the storage of white-brined cheese prepared with or without a starter culture and stored in a 10 or 15% NaCl brine at 10°C and 25°C...
Wed, 06/03/2020 - 03:39
Nisin is applied as a food preservative in processed foods and has the potential to be used synergistically with antibiotics for treatment of patients infected by antibiotic-resistant bacteria, such as methicillin-resistant Staphylococcus aureus. The present study explores the antimicrobial effect of nisin on S. aureus viability and membrane integrity and, for the first time, used super-...
Sun, 05/31/2020 - 03:38
Staphylococcus aureus is one of the most common pathogens that infects the airways of patients with cystic fibrosis (CF) and contributes to respiratory failure. Recently, livestock-associated methicillin-resistant S. aureus (LA-MRSA), usually cultured in farm animals, were detected in CF airways. Although some of these strains are able to establish severe infections in humans, there is limited...
Sat, 05/30/2020 - 03:54
by Rohana P. Dassanayake, Shollie M. Falkenberg, Judith A. Stasko, Adrienne L. Shircliff, John D. Lippolis, Robert E. Briggs Bacterial biofilms are organized sessile communities of bacteria enclosed in extracellular polymeric substances (EPS). To analyze organization of bacteria and EPS in high resolution and high magnification by scanning electron microscopy (SEM), it is important to preserve...
Sat, 05/30/2020 - 03:54
by Stephanie J. Wan, Sophia Ma, David J. Evans, Suzanne M. J. Fleiszig The healthy cornea is remarkably resistant to infection, quickly clearing deliberately inoculated bacteria such as Pseudomonas aeruginosa and Staphylococcus aureus. Contrasting with the adjacent conjunctiva and other body surfaces, it also lacks a resident viable bacterial microbiome. Corneal resistance to microbes depends on...
Sat, 05/30/2020 - 03:46
This paper focused on the determination of the effects of curcumin on extension the shelf life of Oncorhynchus mykiss. The least values of the particle size (10.34 ± 3.8 nm), the total volatile basic nitrogen (11.18 mg/100 g), and the greatest polydispersity index (8.50 ± 0.13) were calculated in a group 5% nanoemulsion after 5 days. Similarly, the least values of total viable bacteria (6.34 ± 0....
Fri, 05/29/2020 - 03:48
Author(s): Jinqiang Hu, Yi Wang, Huimin Ding, Chunpeng Jiang, Yao Geng, Xincheng Sun, Jianzhou Jing, Hui Gao, Zhangcun Wang, Caiwen Dong Currently, rapid, sensitive, and convenient visual detection methods for Staphylococcus aureus (S. aureus) are scarce. In this study, a novel detection method based on recombinase polymerase amplification (RPA) and polymer flocculation sedimentation (PFS) was...
Wed, 05/27/2020 - 03:36
The emergence of bacterial strains resistant to different antibiotics has prompted the search for new sources of antimicrobial compounds. Studies have shown that jambolan [Syzygium cumini (L.) Skeels], a tropical fruit from the Mirtaceae family, contains a great variety of phytochemical compounds with high antioxidant and antimicrobial activity. This study aimed to determine the centesimal...
Sun, 05/24/2020 - 03:37
The occurrence of multidrug-resistant pathogenic bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), multidrug-resistant Acinetobacter baumannii (MDRAB), extended-spectrum β-lactamase (ESBL) Escherichia coli, and Pseudomonas aeruginosa, has become a serious problem in animals and public. The objective of this study was to identify and isolate lactic acid bacterial (LAB) strains...
Fri, 05/22/2020 - 03:36
Streptomyces is the most frequently described genus of Actinomycetes, a producer of biologically active secondary metabolites. Indeed, the Streptomyces species produces about 70% of antibiotics and 60% of antifungal molecules used in agriculture. Our study was carried out with the goal of isolating and identifying antimicrobial secondary metabolites from Streptomyces misionensisV16R3Y1 isolated...
Thu, 05/21/2020 - 03:49
Author(s): Said Kamal Abolghait, Abdelazeem Mohamed Algammal, Aml Gamal Fathi, Fatma Mohamed Youssef Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for several difficult-to-treat infections and staphylococcal food poisoning (SFP). This study was conducted to investigate the prevalence and enterotoxigenicity of MRSA in broiler chicken meat and giblets. A total of 5.5% (8/144) of...
Mon, 05/18/2020 - 03:39
Staphylococcus aureus is an important agent of contagious bovine intramammary infections in dairy cattle. Its ability to persist inside the udder is based on the presence of important mechanisms such as its ability to form biofilms, polysaccharide capsules, small colony variants (SCVs), and their ability to invade professional and non‐professional cells, which will protect S. aureus from the...
Sat, 05/16/2020 - 03:49
by Janine J. Wilden, Andre van Krüchten, Lutz Gieselmann, Eike R. Hrincius, Stefanie Deinhardt-Emmer, Karoline F. Haupt, Hannah F. Preugschas, Silke Niemann, Stephan Ludwig, Christina Ehrhardt Severe influenza virus (IV) infections still represent a major challenge to public health. To combat IV infections, vaccines and antiviral compounds are available. However, vaccine efficacies vary with very...
Fri, 05/15/2020 - 03:46
Staphylococcus aureus can cause mastitis in dairy cattle. We report the genome sequence of a Staphylococcus aureus strain isolated from a dairy cow with a chronic case of mastitis. The infection with this strain of Staphylococcus aureus was not cleared from the animal with antibiotic treatment.

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