Staphylococcus aureus

Fri, 10/11/2019 - 03:30
EchAMP, the tenth most abundant transcript expressed in the mammary gland of echidna, has in vitro broad-spectrum antibacterial effects. However, the effects of EchAMP on mastitis, a condition where inflammation is triggered following mammary gland infection, has not been investigated. To investigate the impact of EchAMP against mastitis, EchAMP transgenic mice were generated. In antibacterial...
Thu, 10/10/2019 - 03:41
ABSTRACT Nonmenstrual toxic shock syndrome (nmTSS), linked to TSST-1-producing CC30 Staphylococcus aureus, is the leading manifestation of toxic shock syndrome (TSS). Due to case rarity and a lack of tractable animal models, TSS pathogenesis is poorly understood. We developed an S. aureus abscess model in HLA class II transgenic mice to investigate pathogenesis and treatment. TSST-1 sensitivity...
Wed, 10/09/2019 - 03:29
During infection, bacterial pathogens successfully sense, respond and adapt to a myriad of harsh environments presented by the mammalian host. This exquisite level of adaptation requires a robust modulation of their physiological and metabolic features. Additionally, virulence determinants, which include host invasion, colonization and survival despite the host's immune responses and...
Sun, 10/06/2019 - 03:29
The chronically inflamed mucosa in patients with chronic rhinosinusitis (CRS) can additionally be infected by bacteria, which results in an acute exacerbation of the disease (AECRS). Currently, AECRS is universally treated with antibiotics following the guidelines for acute bacterial rhinosinusitis (ABRS), as our understanding of its microbiology is insufficient to establish specific treatment...
Sat, 10/05/2019 - 03:28
Treated wastewater from reclaimed facilities (WWTP) has become a reusable source for a variety of applications, such as agricultural irrigation. However, it is also a potential reservoir of clinically-relevant multidrug resistant (MDR) pathogens, including ESKAPE (Enterococcus faecium and Streptococcus surrogates, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas...
Fri, 10/04/2019 - 03:36
LWT
Author(s): Darla Silveira Volcan Maia, Louise Haubert, Isabela Schneid Kroning, Kauana dos Santos Soares, Thaís Larré Oliveira, Wladimir Padilha da SilvaAbstractThe aims of this study were to verify the ability of S. aureus isolates from food poisoning outbreaks to form biofilm, and evaluate the susceptibility of these isolates to Butia odorata extract (BOE) and its effect on S. aureus biofilm....
Fri, 10/04/2019 - 03:29
The aim of this study was to determinate whether coagulase-negative staphylococci (CNS) from buffalo milk or the milking environment possess virulence factors that are associated with intramammary infections or antimicrobial resistance. Milk samples (n = 320) from 80 lactating buffalo were evaluated for clinical and subclinical mastitis by physical examination, the strip cup test, California...
Wed, 10/02/2019 - 03:31
The diffusion of Panton–Valentine leukocidin (PVL)–positive methicillin-resistant S. aureus (MRSA) is a health problem in Algeria. The objectives of the study were to investigate the global distribution of methicillin-susceptible S. aureus (MSSA) and MRSA isolates in different ecological niches in this country. In total, 2246 samples were collected from humans, livestock, wild...
Mon, 09/30/2019 - 17:10
The diffusion of antimicrobial resistance is a significant concern for public health worldwide. Staphylococcus aureus represents a paradigm microorganism for antibiotic resistance in that resistant strains appear within a decade after the introduction of new antibiotics. Methods: Fourteen S. aureus isolates from human specimens and twenty-one from samples of animal origin, were compared for their...
Mon, 09/30/2019 - 17:10
During a six-month period (October 2017–March 2018), the prevalence and susceptibility of important pathogenic bacteria isolated from 12 hospital raw sewage samples in North Western Greece was investigated. The samples were analyzed for methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant enterococci (VRE), extended-spectrum beta-lactamase (ESBL) producing...
Mon, 09/30/2019 - 17:10
Pseudomonas aeruginosa and Staphylococcus aureus are opportunistic pathogens and are commonly found in polymicrobial biofilm-associated diseases, namely chronic wounds. Their co-existence in a biofilm contributes to an increased tolerance of the biofilm to antibiotics. Combined treatments of bacteriophages and antibiotics have shown a promising antibiofilm activity, due to the profound...
Mon, 09/30/2019 - 17:10
Goat milk has been frequently implicated in staphylococcal food poisoning. The potential risk of raw goat milk contaminated by Staphylococcus aureus (S. aureus) in Shaanxi province of China is still not well documented. This study investigated the prevalence, antibiotic resistance, as well as virulence-related genes of S. aureus from raw goat milk samples in Shaanxi, China. A total of 68 S....
Mon, 09/30/2019 - 17:10
For the past few decades, there has been limited progress in the development of novel antibacterials. Previously, we postulated that the gut microbiota of animals residing in polluted environments are a forthcoming supply of antibacterials. Among various species, the water monitor lizard is an interesting species that feeds on organic waste and the carcass of wild animals. Gut microbiota of the...
Mon, 09/30/2019 - 17:03
LWT
Author(s): Alonzo A. Gabriel, Katrina Moira D. Melo, Juan Carlos D. MichelenaAbstractThis study was conducted to determine the efficacy of UV-C irradiation for safety and quality of dried bay leaves. Cocktails of microorganisms of the same species were prepared and inoculated onto the leaves, which were thereafter subjected to UV-C irradiation. Results showed that all tested organisms, which...
Mon, 09/30/2019 - 16:54
ABSTRACT To visualize the personalized distributions of pathogens and chemical environments, including microbial metabolites, pharmaceuticals, and their metabolic products, within and between human lungs afflicted with cystic fibrosis (CF), we generated three-dimensional (3D) microbiome and metabolome maps of six explanted lungs from three cystic fibrosis patients. These 3D spatial maps revealed...
Mon, 09/30/2019 - 16:54
ABSTRACT Mannitol-1-phosphate dehydrogenase (M1PDH) is a key enzyme in Staphylococcus aureus mannitol metabolism, but its roles in pathophysiological settings have not been established. We performed comprehensive structure-function analysis of M1PDH from S. aureus USA300, a strain of community-associated methicillin-resistant S. aureus, to evaluate its roles in cell viability and virulence under...
Sun, 09/29/2019 - 03:31
Author(s): Yu-Xuan Zhang, Jin-Xing Wang, Xian-Wei WangAbstractWhey acidic protein domain (WAPD) is a usual motif in crustaceans, and is found mainly in the immune-related proteins. In the present study, a protein containing three tandem WAPDs was identified in red swamp crayfish Procambarus clarkii and designated as PcTWD. This is the first report of a protein of such domain architecture in...
Sun, 09/29/2019 - 03:31
Author(s): Hengwei Deng, Wenfeng Zhang, Jingjing Li, Jinling Li, Lei Hu, Wenyan Yan, Shanshan Liu, Jianguo He, Shaoping WengAbstractA STAT gene from Scylla paramamosain, named SpSTAT, was cloned and characterized. The full length of SpSTAT mRNA contains a 5′untranslated region (UTR) of 238 bp, an open reading frame (ORF) of 2388 bp and a 3′ UTR of 326 bp. The SpSTAT protein contains four...
Sat, 09/28/2019 - 03:21
Bovine mastitis is an ongoing significant concern in the dairy and agricultural industry resulting in substantial losses in milk production and revenue. Among the predominant etiological agents of bovine mastitis are Staphylococcus aureus, Streptococcus uberis, Streptococcus dysgalactiae, and Escherichia coli. Currently, the treatment of choice for bovine mastitis involves the use of commercial...
Sat, 09/28/2019 - 03:21
Staphylococcus aureus is a commensal colonizer of both humans and animals, but also an opportunistic pathogen responsible for a multitude of diseases. In recent years, colonization of pigs by methicillin resistant S. aureus has become a problem with increasing numbers of humans being infected by livestock strains. In S. aureus colonization and virulence factor expression is controlled by the agr...
Thu, 09/26/2019 - 03:28
LWT
Author(s): Chantelle Human, Dalene de Beer, Marique Aucamp, Isa J. Marx, Christiaan J. Malherbe, Marinda Viljoen-Bloom, Marieta van der Rijst, Elizabeth JoubertAbstractGreen rooibos extract containing high levels of aspalathin, a dihydrochalcone antioxidant, and the carrier chitosan, both an antioxidant and antimicrobial agent, was used to produce a dual-purpose food ingredient powder by spray-...
Tue, 09/24/2019 - 03:40
Glutamate amidation, a secondary modification of the peptidoglycan, was first identified in Staphylococcus aureus. It is catalyzed by the protein products of the murT and gatD genes, which are conserved and colocalized in the genomes of most sequenced Gram-positive bacterial species. The MurT-GatD complex is required for cell viability, full resistance to β-lactam antibiotics, and resistance...
Mon, 09/23/2019 - 03:29
Author(s): Kotková Hana, Cabrnochová Marie, Lichá Irena, Tkadlec Jan, Fila Libor, Bartošová Jana, Melter OtoAbstractBesides natural and acquired mechanisms of resistance, bacteria can cope with presence of antibiotics by using complex mechanisms such as persistence or tolerance.The main purpose of this study was to evaluate the suitability of newly developed Tolerance Disk Test (TDtest) (Gefen et...
Mon, 09/23/2019 - 03:22
The detection of borderline oxacillin-resistant Staphylococcus aureus (BORSA) represents a challenge to both, veterinary and human laboratories. Between 2015 and 2017, 19 equine S. aureus with elevated minimal inhibitory concentrations for oxacillin were detected in routine diagnostics. The aim of this study was to characterize these isolates to identify factors possibly associated with the BORSA...
Sun, 09/22/2019 - 03:31
Staphylococcus aureus has evolved a complex regulatory network that controls a multitude of defense mechanisms against the deleterious effects of oxidative stress stimuli subsequently leading to the pathogen's survival and persistence in the hosts. Previously, we characterized the msaABCR operon as a regulator of virulence, antibiotic resistance, and the formation of persister cells in S. aureus...
Sun, 09/22/2019 - 03:29
Author(s): Yangqun Liu, Changlin Han, Zhiyao Chen, Dan Guo, Xiaohua YeAbstractBackgroundAlthough previous studies have suggested an association between livestock exposure and the risk of methicillin-resistant Staphylococcus aureus (MRSA) carriage in humans, it remains unclear whether there is a dose-response relationship.ObjectiveThe aim of this study was to evaluate the relationship between...
Sun, 09/22/2019 - 03:28
LWT
Author(s): H. Staroszczyk, B. Kusznierewicz, E. Malinowska-Pańczyk, I. Sinkiewicz, K. Gottfried, I. KołodziejskaAbstractThe aim of the work was to study the feasibility of using aqueous extracts from rowanberry, blue-berried honeysuckle, and chokeberry pomace for the formulation of fish gelatin films with antioxidant and antimicrobial activity as well as improved mechanical and water barrier...
Sat, 09/21/2019 - 03:34
Author(s): Ming Li, Chunxia Ma, Peng Zhu, Yanhao Yang, Aiyingi Lei, Xiaohan Chen, Wanwen Liang, Ming Chen, Jianha Xiong, Chaozheng LiAbstractCrustin is an antimicrobial peptide (AMP) that plays a key role in the innate immunity of crustaceans. This study cloned a new crustin from Pacific white shrimp Litopenaeus vannamei, which we designated as LvCrustinB, using rapid amplification of cDNA ends (...
Sat, 09/21/2019 - 03:34
Author(s): Shuang Zhang, Chaozheng Li, Wei Wang, Chenggui Wang, Chengbo Sun, Siuming Chan, Lili ShiAbstractProtein inhibitor of activated STAT (PIAS) plays a critical role in the feedback modulation of the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway as a negative regulator in mammals and Drosophila, but the function of PIAS in crustaceans is still...
Sat, 09/21/2019 - 03:32
Author(s): Maria Luisa Menandro, Giorgia Dotto, Alessandra Mondin, Marco Martini, Letizia Ceglie, Daniela PasottoAbstractThe aim of this study was to assess the prevalence, the genotypic diversity, the antimicrobial resistance traits of canine and feline clinical methicillin-resistant Staphylococcus pseudintermedius (MRSP) isolates in a diagnostic laboratory in Italy during 2015-2016.All isolates...
Sat, 09/21/2019 - 03:32
Author(s): Gamal A Elmowalid, Marwa I Abd El-Hamid, Ashraf M Abd El-Wahab, Mohammad Atta, Gamal Abd El-Naser, Adel M AttiaAbstractAdministration of antibiotics as feed additives in broilers resulted in prompting of some undesirable effects such as the rising emergence of multi-drug resistant (MDR) bacteria, so scrutinizing for new alternatives like herbs is the up to date task for global health....
Sat, 09/21/2019 - 03:32
Author(s): J. Filipe, V. Bronzo, G. Curone, B. Castiglioni, D. Vigo, B. Smith, V. Herrera, P. Roccabianca, P. Moroni, F. RivaAbstractIL-1R8 is a member of Interleukin-1 receptor family acting as a negative regulator of inflammation reliant on ILRs and TLRs activation. IL-1R8 role has never been evaluated in acute bacterial mastitis. We first investigated IL-1R8 sequence conservation among...
Sat, 09/21/2019 - 03:32
Author(s): Juliana Marzari Rossato, Terimar Ruoso Moresco, Juliano Uczay, João Batista Teixeira da RochaAbstractInvertebrates have been instrumental in understanding the mechanisms involved in infectious diseases, considering the idea to replace, reduce and refine the use of mammals as well as to understand the basic principles of immune response in insect. We evaluated the consequences of...
Sat, 09/21/2019 - 03:30
The occurrence of multidrug‐resistant zoonotic bacteria in animals has been increasing worldwide. Working in close contact with livestock increases the risk of carriage of these bacteria. We investigated the occurrence of extended‐spectrum beta‐lactamase (ESBL) and plasmidic AmpC beta‐lactamase producing Enterobacteriaceae (ESBL/pAmpC‐PE) and livestock‐associated methicillin‐resistant...
Sat, 09/21/2019 - 03:29
An 8‐week feeding trial with juvenile hybrid tilapia (Oreochromis niloticus × Oreochromis aureus) was conducted to assess the effects of dietary phenylalanine on growth, body composition, and biochemical parameters in plasma. Six diets were formulated with graded levels of l‐phenylalanine (0.43, 0.74, 1.01, 1.30, 1.60, and 1.91% of the diet). Each diet was randomly assigned to triplicate groups...
Sat, 09/21/2019 - 03:27
Aims To assess the bacterial diversity in the French egg processing industry and to explore the adhesion and spoilage potential of selected bacteria. Methods and Results Sterile stainless steel chips were suspended for 2 months inside the pipelines of seven egg processing companies, before and after the pasteurizer, at warm and cold seasons. After exposure, the bacterial diversity was assessed by...
Sat, 09/21/2019 - 03:26
BACKGROUND The scarcity of irrigation water is severely affecting global crop production. In this context, biostimulants are increasingly used as alternatives means against abiotic stress conditions. In this study, phenolic compounds composition and bioactive properties of common bean (Phaseolus vulgaris L.) plants grown under water stress conditions and biostimulants application were...
Sat, 09/21/2019 - 03:25
Staphylococcus aureus is part of the normal flora of animals, and represents one of the leading causes of contagious mastitis in dairy herds worldwide. Sixty-seven epidemiologically unrelated S. aureus isolates from nasal and mastitis milk samples of dairy-producing animals (32 cows, 25 sheep, and 10 goats) were characterized by antimicrobial susceptibility testing and spa typing followed by...
Sat, 09/21/2019 - 03:25
Municipal wastewater treatment plants (WWTPs) may serve as a reservoir for potentially pathogenic and antibiotic resistant bacteria. The discharge of improperly treated wastewater effluent may lead to the spread of these bacteria, including methicillin-resistant Staphylococcus aureus (MRSA) which is responsible for causing pneumonia, septicaemia and skin and soft tissue infections, into the...
Sat, 09/21/2019 - 03:25
Bacterial biofilms are multicellular aggregates enclosed in a self-created biopolymer matrix. Biofilm-producing bacteria have become a great public health problem worldwide because biofilms enable these microorganisms to evade several clearance mechanisms produced by host and synthetic sources. Over the past years, different flavonoids including quercetin have engrossed considerable interest...
Sat, 09/21/2019 - 03:24
This study aimed to optimize the extraction conditions for Sorbus umbellata (Desf.) Fritsch var. umbellata leaves to maximize the phenolic content and their antioxidant activity and to investigate β-glucuronidase (GUS) enzyme inhibitory, antimicrobial and cytotoxic potentials of the extracts obtained under optimum conditions. The optimum extraction conditions were found to be 78.2 and 79.7%...
Sat, 09/21/2019 - 03:24
Coagulase-negative staphylococci (CNS) are one of the most common bovine mastitis pathogens found worldwide. In this study, we investigated the prevalence and distribution of CNS species in mastitis milk samples and further characterized the methicillin-resistant (MR) CNS. A total of 311 CNS were isolated from 3,692 quarter milk samples from 1,373 dairy cattle at 81 farms between 2013 and 2017....
Fri, 09/20/2019 - 03:42
Author(s): Farina E. Lennartz, Bianca Schwartbeck, Angelika Dübbers, Jörg Große-Onnebrink, Christina Kessler, Peter Küster, Holger Schültingkemper, Georg Peters, Barbara C. KahlAbstractBackground: Staphylococcus aureus is one of the most frequently isolated pathogens in the respiratory tract of CF patients. Recently, we characterized peculiar mucoid S. aureus isolates, which are excessive biofilm...
Fri, 09/20/2019 - 03:42
Author(s): Dinglong Yang, Yijing Han, Lizhu Chen, Ruiwen Cao, Qing Wang, Zhijun Dong, Hui Liu, Xiaoli Zhang, Qianqian Zhang, Jianmin ZhaoAbstractBactericidal permeability-increasing protein (BPI) is an antimicrobial protein with potent endotoxin-neutralising activity and plays a crucial role in innate immunity against bacterial infection. In the present study, a bpi (designed as rpbpi) was...
Fri, 09/20/2019 - 03:41
Author(s): Angeliki Angelopoulou, Ross Holohan, Mary C. Rea, Alicja K. Warda, Colin Hill, R. Paul RossAbstractBovine mastitis, an inflammation of the udder, is associated with increases in milk somatic cell count usually resulting from bacterial infection. We analysed 50 mastitic milk samples via cultivation, 16S rRNA sequencing and a combination of the two (culturomics) to define the complete...
Fri, 09/20/2019 - 03:40
Author(s): Tanya Clements, Thando Ndlovu, Wesaal KhanAbstractThe genus Serratia is a predominantly unexplored source of antimicrobial secondary metabolites. The aim of the current study was thus to isolate and evaluate the antimicrobial properties of biosurfactants produced by Serratia species. Forty-nine (n = 34 pigmented; n = 15 non-pigmented) biosurfactant producing Serratia strains were...
Fri, 09/20/2019 - 03:39
Author(s): Sirlei G. Marques, Maria Rosa Q. Bomfim, Conceição de Maria P.S. Azevedo, Cleide Viviane B. Martins, Ana Claudia G. Marques, Azizedite G. Gonçalves, Vania A. Vicente, Alcione M. dos Santos, Marliete C. Costa, Gustavo J.C. Freitas, Daniel A. Santos, Maria A. de Resende-StoianoffAbstractChromoblastomycosis (CBM) is a chronic subcutaneous infection caused by melanotic fungi, affecting...

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