Staphylococcus aureus

Fri, 04/03/2020 - 03:34
Author(s): Isabela Schneid Kroning, Louise Haubert, Natalie Rauber Kleinubing, Itiane Barcellos Jaskulski, Letícia Klein Scheik, Tassiana Ramires, Wladimir Padilha da Silva The genetic diversity by spa typing, the susceptibility to the sanitisers benzalkonium chloride (BC) and chlorhexidine (CHL) and the presence of efflux pump genes to resistance to sanitisers in 31 Staphylococcus aureus...
Fri, 04/03/2020 - 03:27
Biofilm formation in S. aureus may reduce the rate of penetration of antibiotics, thereby complicating treatment of infections caused by these bacteria. The aim of this study was to correlate biofilm-forming potentials, antimicrobial resistance, and genes in S. aureus isolates. Methods: A total of 64 milk samples were analysed, and 77 S. aureus were isolated. Results: Seventy (90.9%) isolates...
Fri, 04/03/2020 - 03:27
The global spread of antibiotic resistance is forcing the scientific community to find new molecular strategies to counteract it. Deep functional profiling of microbiomes provides an alternative source for the discovery of novel antibiotic producers and probiotics. Recently, we implemented this ultrahigh-throughput screening approach for the isolation of Bacillus pumilus strains efficiently...
Thu, 04/02/2020 - 03:34
In dairy plants, clean-in-place (CIP) apparatus cannot be disassembled and, therefore, it is not easy to clean the inner surface of their pipes. In this study, the inhibitory effects of chemical agents on biofilms formed by three foodborne pathogens, Bacillus cereus , Escherichia coli , and Staphylococcus aureus , were evaluated in a dairy CIP system. First, the experiment was conducted on a...
Thu, 04/02/2020 - 03:33
This study examined the microbicidal activity of 222-nm UV radiation (UV222), which is potentially a safer alternative to the 254-nm UV radiation (UV254) that is often used for surface decontamination. Spores and/or growing and stationary-phase cells of Bacillus cereus, Bacillus subtilis, Bacillus thuringiensis, Staphylococcus aureus, and Clostridioides difficile and a herpesvirus were all killed...
Thu, 04/02/2020 - 03:33
Nisin A is a bacteriocin produced by Lactococcus lactis and is widely used as a food preservative. Staphylococcus aureus has the BraRS-VraDE system that provides resistance against low concentrations of nisin A. BraRS is a two-component system that induces the expression of the ABC transporter VraDE. Previously, we isolated a highly nisin A-resistant strain with increased VraDE expression due to...
Thu, 04/02/2020 - 03:33
The wastewater of livestock slaughterhouses is considered a source of antimicrobial-resistant bacteria with clinical relevance and may thus be important for their dissemination into the environment. To get an overview of their occurrence and characteristics, we investigated process water (n = 50) from delivery and unclean areas as well as wastewater (n = 32) from the in-house wastewater treatment...
Thu, 04/02/2020 - 03:29
Edible packaging films have been widely studied because of its safety, green, and effective characteristics. In this paper, chitosan (CH) edible films containing hexahydro‐β‐acids (HBA) were prepared, and its physical and mechanical properties, bioactivity, and their impact on the shelf life of pork were investigated. The infrared spectra indicated that the molecular interaction between CH and...
Wed, 04/01/2020 - 03:27
Anti‐digestive enzyme, antioxidant, anti‐proliferative and antibacterial activities of the extract from Moringa oleifera at different extraction temperatures. Summary In an ultrasonic bath and for 30 min in the presence of water as extraction solvent in a ratio of 1:10 (g/mL), effect of extraction temperature on extraction of caffeoylquinic acids (CQAs) from Moringa oleifera leaves (MOL) was...
Tue, 03/31/2020 - 03:26
Both the ability of bacteria to form biofilms and communicate through quorum sensing allows them to develop different survival or virulence traits that lead to increased bacterial resistance against conventional antibiotic therapy. Here, seventeen essential oils (EOs) were investigated for the antimicrobial, antibiofilm, and anti-quorum sensing activities on Escherichia. coli O157:H7, Escherichia...
Sat, 03/28/2020 - 03:24
Automatic flushing of milking clusters between milking events is a control measure aimed at reducing transmission of mastitis pathogens from infectious milk to a subsequently milked cow. We evaluated the effect of flushing with cold water and flushing with water containing peracetic acid (PAA) on the concentration of Staphylococcus aureus in teat cup liners. Thirty-two clusters in a swing-over...
Fri, 03/27/2020 - 03:42
In countries with intensive pig husbandry in stables, the prevalence of livestock-associated (LA) methicillin-resistant Staphylococcus aureus (MRSA) on such farms has remained high in the last few years or has also further increased. Simple measures to reduce the LA-MRSA among pigs have not yet been successfully implemented. Earlier publications showed a decontamination of LA-MRSA was only...
Thu, 03/26/2020 - 03:34
Rapid identification from positive blood cultures is standard of care (SOC) in many clinical microbiology laboratories. The GenMark Dx ePlex Blood Culture Identification Gram-Positive (BCID-GP) Panel is a multiplex nucleic acid amplification assay based on competitive DNA hybridization and electrochemical detection using eSensor technology. This multicenter study compared the investigational-use-...
Thu, 03/26/2020 - 03:34
Rapid diagnostic tests (RDTs) for bloodstream infections (BSIs) decrease the time to organism identification and resistance detection. RDTs are associated with early deescalation of therapy for Gram-positive BSIs. However, it is less clear how RDTs influence antibiotic management for Gram-negative BSIs and whether RDT results are acted on during off-hours. We performed a single-center,...
Sat, 03/21/2020 - 03:26
Staphylococcus aureus is one of the main causative agents of food poisoning. This bacterium is an important component of cheese microbiota and plays an important role in foodborne diseases. Another important component of the microbiota is the lactic acid bacterium, which actively participates in processes that define the physicochemical, sensorial, and microbiological features of cheese. Of the...
Thu, 03/19/2020 - 03:26
The anodized titanium nanotubes (TiO2-NTs) are considered to be a potential material in clinical therapy. To enhance the antibacterial property of TiO2-NTs, cefuroxime is introduced into TiO2-NTs, and then, different chitosan layers are coated to control the release of cefuroxime. SEM and FTIR are adapted for the characterization of prepared TiO2-NTs. The effects of chitosan coating thickness on...
Thu, 03/19/2020 - 03:25
Eugenia brejoensis L. (Myrtaceae) is an endemic plant from caatinga ecosystem (brazilian semi-arid) which have an E. brejoensis essential oil (EbEO) with reported antimicrobial activity. In this work, in vitro and in vivo models were used to characterize the inhibitory effects of EbEO in relation to Staphylococcus aureus. EbEO inhibited the growth of all tested S. aureus strains (including...
Wed, 03/18/2020 - 03:25
Photodynamic antimicrobial chemotherapy (PAC) is an efficient tool for inactivating microorganisms. This technique is a good approach to inactivate the foodborne microorganisms, which are responsible for one of the major public health concerns worldwide—the foodborne diseases. In this work, response surface methodology (RSM) was used to evaluate the interaction of Eosin Y (EOS)...
Tue, 03/17/2020 - 03:26
Sequence type 59 (ST59) is a predominant clonal lineage of community-acquired, methicillin-resistant Staphylococcus aureus (CA-MRSA) in Asia. Despite its increasing clinical relevance in China, the evolution and geographic expansion of ST59 has been relatively uncared for. Previous study has shown that ST59 was the predominant clone in food-related MRSA in China. This study compared the genomes...
Sun, 03/15/2020 - 04:01
Author(s): Taekeon Jung, Yugyung Jung, Jaehyeon Ahn, Sung Yang Most biosensors use small amounts (a few tens of microliters) of a sample to detect bacteria. However, it is necessary to detect bacteria with at least a few milliliters of the sample to obtain statistically significant results. Therefore, the bacteria concentration process is critical for the rapid and accurate detection of bacteria...
Fri, 03/13/2020 - 03:24
Staphylococcus aureus superantigens (SAgs) are among the most potent T cell mitogens known. They stimulate large fractions of T cells by cross-linking their T cell receptor with major histocompatibility complex class-II molecules on antigen presenting cells, resulting in T cell proliferation and massive cytokine release. To date, 26 different SAgs have been described in the species S. aureus;...
Thu, 03/12/2020 - 03:36
by Lene Bungenstock, Amir Abdulmawjood, Felix Reich With regards to the frequently reported findings of spoilage bacteria and pathogens in various foods there is a need to explore new ways to control hazards in food production and to improve consumer safety. Fermented sausages from traditional and industrial production in Germany were screened for lactic acid bacteria with antibacterial effects...
Thu, 03/12/2020 - 03:28
Emerging technologies in the meat industry. Summary This study investigated the effect of the combination of basic electrolysed water (BEW) and slightly acid electrolysed water (SAEW) with ultrasound (US) for cleaning and sanitation of the knives used in slaughtering processes. The knives were sonicated in a US bath using two modes of operation (normal and sweep) in two steps: (i) 5 min with BEW...
Wed, 03/11/2020 - 03:33
Author(s): Hwan Hee Yu, Ye Ji Song, Yeon Jin Kim, Hee Young Lee, Yun-Sang Choi, Na-Kyoung Lee, Hyun-Dong Paik This study describes a model of the growth kinetics for S. aureus in raw beef under wrapped packaging (WP), modified atmosphere packaging (MAP), vacuum packaging (VP), and vacuum skin packaging (VSP). Beef samples were inoculated with S. aureus and stored at 10, 15, 20, and 25 °C. VP and...
Wed, 03/11/2020 - 03:27
Activity-guided fractionation based on in vitro assays was used to identify antibacterial components in the residue wasted in the manufacturing process of aged garlic extract (AGE). Antimicrobial activity of the hexane extract of aged garlic-derived residue (AGR) was evaluated against clinically isolated Gram-positive and -negative bacteria including levofloxacin (LVX)-resistant strains. Among...
Wed, 03/11/2020 - 03:26
Sea turtles can be considered a sentinel species for monitoring the health of marine ecosystems, acting, at the same time, as a carrier of microorganisms. Indeed, sea turtles can acquire the microbiota from their reproductive sites and feeding, contributing to the diffusion of antibiotic-resistant strains to uncontaminated environments. This study aims to unveil the presence of antibiotic-...
Tue, 03/10/2020 - 03:34
by Saori Oku, Toru Takeshita, Toshiko Futatsuki, Shinya Kageyama, Mikari Asakawa, Yasuo Mori, Toshihiro Miyamoto, Jun Hata, Toshiharu Ninomiya, Haruhiko Kashiwazaki, Yoshihisa Yamashita Disruption of the intestinal microbiota caused by intensive chemotherapy, irradiation and antibiotics can result in development of severe gut graft-versus-host disease and infectious complications, leading to...
Sun, 03/08/2020 - 04:35
Antibiotic resistance is a worldwide threat due to the decreasing supply of new antimicrobials. Novel targets and innovative strategies are urgently needed to generate pathbreaking drug compounds. NAD kinase (NADK) is essential for growth in most bacteria, as it supports critical metabolic pathways. Here, we report the discovery of a new class of antibacterials that targets bacterial NADK. We...
Sun, 03/08/2020 - 04:32
Author(s): Daniele Chieffi, Francesca Fanelli, Gyu-Sung Cho, Justyna Schubert, Giuseppe Blaiotta, Charles M.A.P. Franz, Jacek Bania, Vincenzina Fusco In this study, 53 Staphylococcus (S.) aureus strains were typed by 16S–23S rDNA intergenic spacer region (ISR) typing and staphylococcal enterotoxin gene (SEg) typing for all the staphylococcal enterotoxin (se) and staphylococcal enterotoxin-like...
Wed, 03/04/2020 - 03:54
The use of nutmeg essential oil‐loaded sage seed mucilage‐based coating with oxygen barrier effect along with antioxidant and antimicrobial activity enhanced the beef shelf‐life by lowering the moisture losses, microbial growth rate, and oxidation reaction progression, as well as by improving the sensory properties of the product. Therefore, the nutmeg essential loaded edible coating with...
Wed, 03/04/2020 - 01:19
The microbiological quality of ready-to-eat (RTE) foods from school cafeterias in Chongqing, China, was evaluated in comparison to a guideline published by a provincial health commission. Based on their preparation processes and potential risks, the RTE foods were divided into 5 types: food type 1, general cooked and hot-held foods; food type 2, cooked meats; food type 3, heated aquatic products...
Wed, 03/04/2020 - 01:19
Canadian First Nations communities rely on traditional preservation methods such as  the smoking, drying, and canning of fish and game meats, to ensure long-term food security. Unlike commercial food production, there are no recognized official standards for these methods, thus rendering their efficacy in producing microbiologically safe foods relatively unknown. In this study, 81 fresh or...
Wed, 03/04/2020 - 01:18
Foods that are extensively handled during preparation and stored without refrigeration are often associated with staphylococcal food poisoning. This problem is more confounding when contaminating strains belong to the methicillin-resistant Staphylococcus aureus (MRSA) group. In this study, we investigated the survivability of methicillin-resistant S. aureus (MRSA) in two different seafood...
Sun, 03/01/2020 - 03:35
Author(s): J. Rocha-Pimienta, S. Martillanes, R. Ramírez, J. Garcia-Parra, J. Delgado-Adamez The effect of high hydrostatic pressure (HHP) on pathogen microorganisms is influenced by an important number of variables like treatment conditions, microorganism type and food matrix, among others. This technology could be an alternative to pasteurization in Human Milk Banks. In this study, a response...
Sat, 02/29/2020 - 03:28
Antibiotic resistance/tolerance has become a severe threat to human and animal health. To combat antibiotic-resistant/tolerant bacteria, it is of significance to improve the efficacy of traditional antibiotics. Here we show that indole potentiates tobramycin to kill stationary-phase Staphylococcus aureus cells after a short, combined treatment, with its derivative 5-methylindole being the most...
Fri, 02/28/2020 - 03:31
This research examined the effects of microwave and hot air oven drying on the nutritional, microbiological load, and color parameters of crickets’ powder. Both drying methods resulted in a slight variation in proximate composition. Microwave drying was considered the most suitable method for producing crickets’ powder because of the high levels of mineral elements, improvement in color...
Thu, 02/27/2020 - 03:46
Staphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for controlling mastitis are needed that depend on a better knowledge of cow-to-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies...
Thu, 02/27/2020 - 03:46
In this study, we established a rapid, simple, and sensitive method for visual and point-of-care detection of Salmonella spp., Cronobacter spp., and Staphylococcus aureus in powdered infant formula (PIF) based on multiplex loop-mediated isothermal amplification (mLAMP) combined with lateral flow dipstick (LFD). Three different species-specific target genes, siiA of Salmonella spp., internal...
Thu, 02/27/2020 - 03:46
Staphylococcus aureus is an important intramammary pathogen for dairy cows that also is remarkably important for public health. Multiple virulence factors can be involved simultaneously during the pathogenesis of a staphylococcal disease, including adhesion proteins, extracellular enzymes, and toxins. The main objective of this study was to assess virulence factors that are associated with cow...
Wed, 02/26/2020 - 03:36
ABSTRACT Staphylococcus epidermidis is a leading cause of nosocomial infections in patients with a compromised immune system and/or an implanted medical device. Seventy to 90% of S. epidermidis clinical isolates are methicillin resistant and carry the mecA gene, present in a mobile genetic element (MGE) called the staphylococcal cassette chromosome mec (SCCmec) element. Along with the presence of...
Wed, 02/26/2020 - 03:36
ABSTRACT Many patients with severe infections receive inappropriate empirical treatment, and rapid detection of bacterial antibiotic susceptibility can improve clinical outcome and reduce mortality. To this end, we have developed a multiplex fluidic chip for rapid phenotypic antibiotic susceptibility testing of bacteria. A total of 21 clinical isolates of Escherichia coli, Klebsiella pneumoniae,...
Tue, 02/25/2020 - 03:30
It is well-established that the spread of many multidrug-resistant (MDR) bacteria is predominantly clonal. Interestingly the international clones/sequence types (STs) of most pathogens emerged and disseminated during the last three decades. Strong experimental evidence from multiple laboratories indicate that diverse fitness cost associated with high-level resistance to fluoroquinolones...
Sat, 02/22/2020 - 03:39
Antibiotic tolerance is an underappreciated antibiotic escape strategy that is associated with recurrent and relapsing infections, as well as acting as a precursor to resistance. Tolerance describes the ability of a bacterial population to survive transient exposure to an otherwise lethal concentration of antibiotic without exhibiting an elevated MIC. It is detected in time-kill assays as a lower...
Sat, 02/22/2020 - 03:39
The threat of diminished antibiotic discovery has global health care in crisis. In the United States, it is estimated each year that over 2 million bacterial infections are resistant to first-line antibiotic treatments and cost in excess of 20 billion dollars. Many of these cases result from infection with the ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae,...
Sat, 02/22/2020 - 03:28
In Rhizobium-legume symbiosis, the bacteria are converted into nitrogen-fixing bacteroids. In many legume species, differentiation of the endosymbiotic bacteria is irreversible, culminating in definitive loss of their cell division ability. This terminal differentiation is mediated by plant peptides produced in the symbiotic cells. In Medicago truncatula more than ∼700 nodule-specific cysteine-...
Fri, 02/21/2020 - 04:14
Author(s): Inês Azevedo, Joana Barbosa, Helena Albano, Paula TeixeiraAbstractIn response to nutritional and health concerns, the food industry has begun to offer a wider variety of products that reflect changing consumer preferences. In addition to traditional alheiras, made with pork and/or poultry meats, other varieties of alheiras (“innovative”) made from codfish, mushrooms, tofu, soy and...
Fri, 02/21/2020 - 04:06
LWT
Author(s): Pochanart Kanjan, Phat SakpetchAbstractThe objective of this study was to examine the safety and functional properties of Staphylococcus simulans PMRS35 isolated from Budu. This strain possessed good lipase activity and moderate protease activity, tolerated 25% of salt, and did not exhibit decarboxylase activity. In addition, this strain displayed not only negative results towards...
Fri, 02/21/2020 - 04:00
A key unmet food safety need remains the objective assessment of human milk spoilage. Experiments were conducted using a simplified human milk spoilage model based on goat's milk as a human milk surrogate, spiked with a single bacterial strain (S. epidermidis), in which pH and carbon dioxide (CO2) concentration were measured along with bacteria count over 160 hr. Bacteria count correlated highly...
Fri, 02/21/2020 - 03:51
Staphylococcus aureus is one of the most common pathogens associated with bovine mastitis in Germany and Denmark. Successful therapy is strongly linked to the susceptibility of the pathogen to the administered antimicrobial. An increase in resistant pathogens in human and veterinary medicine has become a concern worldwide and hampers therapy due to reduced susceptibility. In the present study,...
Fri, 02/21/2020 - 03:51
Passion fruit (Passiflora cincinnata Mast.) is a native fruit from the Caatinga, a typical ecoregion in northeastern Brazil, and it has potential for use by the food and pharmaceutical industries. In this study, we characterized the antimicrobial activity of P. cincinnata and its application in the production of coalho cheese, a traditional Brazilian product. Aqueous extract of P. cincinnata...

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